ORF » Species Summary » Leishmania major strain Friedlin » LMJF_05_0500 cDNA ORF clone

LMJF_05_0500 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_05_0500 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_05_0500 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb00170 XM_001687475.1
Latest version!		 Leishmania major strain Friedlin ATPase alpha subunit partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_05_0500 ( XM_001687475.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001687527.1 Leishmania major strain Friedlin ATPase alpha subunit partial mRNA.
    Price & Availability↑
    CloneID OLb00170
    Clone ID Related Accession (Same CDS sequence) XM_001687475.1
    Accession Version XM_001687475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product ATPase alpha subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007246). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCGCCGCT TCGTGGCCCA GTACGTGGCG CCCGCCATGG GACGCCTTGC GTCGACGGCT 
    GCTGCCGGCA AGTCTGCCGC GCCGGGCCAG AAGTCGTTCT TCAAGGCGAC GGAGATGATC 
    GGCTACGTGC ACTCGATCGA CGGCACGATC GCGACGCTGA TCCCCGCGCC GGGCAACCCC 
    GGCGTTGCGT ACAACACGAT CATCATGATC CAGGTGAGCC CGACGACGTT CGCGGCGGGG 
    CTTGTGTTCA ACCTGGAGAA GGACGGCCGG ATAGGCATCA TCCTGATGGA TAACATCACG 
    GAGGTGCAGT CCGGCCAGAA GGTGATGGCG ACGGGCAAGC TGCTGTACAT CCCCGTGGGT 
    GCGGGCGTGC TGGGCAAGGT GGTGAACCCG CTGGGCCACG AGGTGCCGGT GGGGCTGTTG 
    ACGCGGTCGC GCGCGCTGCT GGAGAGCGAA CAGACGCTGG GCAAGGTGGA CGCCGGCGCG 
    CCGAACATCG TGTCGCGCTC GCCAGTGAAC TACAACCTGC TGACCGGCTT CAAGGCAGTG 
    GACACGATGA TCCCGATCGG GCGCGGCCAG CGCGAGCTGA TCGTGGGTGA CCGCCAGACC 
    GGCAAGACGT CGATCGCGGT GTCGACGATC ATCAACCAGG TGCGCAGCAA CCAGCAGATC 
    CTATCGAAGA ACGCGGTCAT CTCGATCTAC GTGTCGATCG GGCAGCGCTG CTCCAACGTC 
    GCGCGCATCC ACCGCCTGCT GCGCTCGTAC GGCGCGCTGC GCTACACGAC GGTGATGGCT 
    GCGACGGCCG CGGAGCCGGC GGGGCTGCAG TACCTCGCGC CGTACTCGGG CGTGACAATG 
    GGCGAGTACT TCATGAACCG CGGCCGCCAC TGCCTGTGTG TGTACGACGA CCTGTCGAAG 
    CAAGCCGTTG CGTACCGCCA GATCTCGCTG CTGCTGCGCC GCCCGCCGGG CCGCGAGGCG 
    TACCCTGGTG ATGTGTTCTA CCTGCACTCG CGCCTGCTGG AGCGCGCCGC GATGCTGTCG 
    CCTGGCAAGG GCGGCGGCTC CGTGACGGCG CTGCCGATCG TGGAGACGCT GTCGAACGAT 
    GTGACGGCGT ACATCGTCAC GAACGTCATC TCCATCACGG ACGGCCAGAT CTACCTGGAC 
    ACGAAGCTGT TCACCGGCGG CCAGCGCCCG GCCGTGAACA TCGGCCTGTC CGTGTCGCGC 
    GTCGGCTCGT CCGCGCAGAA CGTGGCGATG AAGGCGGTGG CCGGCAAGCT GAAGGGCATC 
    CTCGCGGAGT ACCGCAAGCT GGCGGCGGAC TCGGTGGGCG GGAGCCAGGT GCAGACGGTG 
    CCGATGATCC GCGGCGCGCG CTTCGTCGCG CTGTTCAACC AGAAGAACCC GTCCTTCTTC 
    ATGAACGCGC TCGTGTCGCT GTACGCGTGC CTGAACGGGT ACCTGGACGA CGTGAAGGTG 
    AACTACGCGA AGCTCTACGA GTACCTGCTG GTGAACAAGG ACCTGAGCGT GATGTACGGG 
    ACAGCGACGA ACAAGTTCTT CTACATGTAC GTGCAGCAGC TGAACTACGT GATCCGCTTC 
    TTCACGCTGA ACCACCCGAT CCTGAACGCG GAGGTGGAGG AGATGCTGAA GCAGCACACG 
    CACCTGTTCC TGCAGCACTA CCAGTCGAAG ATGAACGCGA TCAAGACGGA GAAGGAGATC 
    AAGGCGCTCA AGAACCTGCT GTACTCATGC AAGCGCGCCG TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001687527.1
    CDS1..1725
    Translation

    Target ORF information:

    RefSeq Version XM_001687475.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin ATPase alpha subunit partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001687475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCGCCGCT TCGTGGCCCA GTACGTGGCG CCCGCCATGG GACGCCTTGC GTCGACGGCT 
    GCTGCCGGCA AGTCTGCCGC GCCGGGCCAG AAGTCGTTCT TCAAGGCGAC GGAGATGATC 
    GGCTACGTGC ACTCGATCGA CGGCACGATC GCGACGCTGA TCCCCGCGCC GGGCAACCCC 
    GGCGTTGCGT ACAACACGAT CATCATGATC CAGGTGAGCC CGACGACGTT CGCGGCGGGG 
    CTTGTGTTCA ACCTGGAGAA GGACGGCCGG ATAGGCATCA TCCTGATGGA TAACATCACG 
    GAGGTGCAGT CCGGCCAGAA GGTGATGGCG ACGGGCAAGC TGCTGTACAT CCCCGTGGGT 
    GCGGGCGTGC TGGGCAAGGT GGTGAACCCG CTGGGCCACG AGGTGCCGGT GGGGCTGTTG 
    ACGCGGTCGC GCGCGCTGCT GGAGAGCGAA CAGACGCTGG GCAAGGTGGA CGCCGGCGCG 
    CCGAACATCG TGTCGCGCTC GCCAGTGAAC TACAACCTGC TGACCGGCTT CAAGGCAGTG 
    GACACGATGA TCCCGATCGG GCGCGGCCAG CGCGAGCTGA TCGTGGGTGA CCGCCAGACC 
    GGCAAGACGT CGATCGCGGT GTCGACGATC ATCAACCAGG TGCGCAGCAA CCAGCAGATC 
    CTATCGAAGA ACGCGGTCAT CTCGATCTAC GTGTCGATCG GGCAGCGCTG CTCCAACGTC 
    GCGCGCATCC ACCGCCTGCT GCGCTCGTAC GGCGCGCTGC GCTACACGAC GGTGATGGCT 
    GCGACGGCCG CGGAGCCGGC GGGGCTGCAG TACCTCGCGC CGTACTCGGG CGTGACAATG 
    GGCGAGTACT TCATGAACCG CGGCCGCCAC TGCCTGTGTG TGTACGACGA CCTGTCGAAG 
    CAAGCCGTTG CGTACCGCCA GATCTCGCTG CTGCTGCGCC GCCCGCCGGG CCGCGAGGCG 
    TACCCTGGTG ATGTGTTCTA CCTGCACTCG CGCCTGCTGG AGCGCGCCGC GATGCTGTCG 
    CCTGGCAAGG GCGGCGGCTC CGTGACGGCG CTGCCGATCG TGGAGACGCT GTCGAACGAT 
    GTGACGGCGT ACATCGTCAC GAACGTCATC TCCATCACGG ACGGCCAGAT CTACCTGGAC 
    ACGAAGCTGT TCACCGGCGG CCAGCGCCCG GCCGTGAACA TCGGCCTGTC CGTGTCGCGC 
    GTCGGCTCGT CCGCGCAGAA CGTGGCGATG AAGGCGGTGG CCGGCAAGCT GAAGGGCATC 
    CTCGCGGAGT ACCGCAAGCT GGCGGCGGAC TCGGTGGGCG GGAGCCAGGT GCAGACGGTG 
    CCGATGATCC GCGGCGCGCG CTTCGTCGCG CTGTTCAACC AGAAGAACCC GTCCTTCTTC 
    ATGAACGCGC TCGTGTCGCT GTACGCGTGC CTGAACGGGT ACCTGGACGA CGTGAAGGTG 
    AACTACGCGA AGCTCTACGA GTACCTGCTG GTGAACAAGG ACCTGAGCGT GATGTACGGG 
    ACAGCGACGA ACAAGTTCTT CTACATGTAC GTGCAGCAGC TGAACTACGT GATCCGCTTC 
    TTCACGCTGA ACCACCCGAT CCTGAACGCG GAGGTGGAGG AGATGCTGAA GCAGCACACG 
    CACCTGTTCC TGCAGCACTA CCAGTCGAAG ATGAACGCGA TCAAGACGGA GAAGGAGATC 
    AAGGCGCTCA AGAACCTGCT GTACTCATGC AAGCGCGCCG TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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