CRISPR in Creating Knockin Cell Lines and Animal Models - Functionalizing Genome Editing for a Broad Range of Targets

Genome Engineering allows the easy manipulation of genomes down to the nucleotide level. CRISPR is a revolutionizing tool for generating custom edited cell and pre-clinical animal models for research. Targeted deep sequencing enables the detection and quantification of low-frequency editing events. However, the large amounts of data generated by targeted deep sequencing can be difficult to interpret and quickly analyze. We have developed a Python-based computer program called CRIS.py that allows the easy analysis of multiple types of editing events. We will show examples of how rapid deep sequence analysis has guided experimental design leading to high-efficiency genome editing in a broad range of applications. Additionally, we will discuss best practices for creating knockin cell and animal models including large insertions using long ssDNA.

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CRISPR in Creating Knockin Cell Lines and Animal Models - Functionalizing Genome Editing for a Broad Range of Targets

Genome Engineering allows the easy manipulation of genomes down to the nucleotide level.

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