kdm5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	kdm5b
Entrez Gene ID	100488170
Full Name	lysine demethylase 5B
Synonyms	jarid1b
General protein information	Preferred Names lysine demethylase 5B Names lysine-specific demethylase 5B lysine (K)-specific demethylase 5B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002939638.5	XP_002939684.2	lysine-specific demethylase 5B isoform X2
XM_018091505.2	XP_017946994.1	lysine-specific demethylase 5B isoform X1
XM_018091505.1	XP_017946994.1	lysine-specific demethylase 5B isoform X1
XM_002939638.4	XP_002939684.2	lysine-specific demethylase 5B isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KDM5B	XP_002694248.2
Rattus norvegicus (Norway rat)	Kdm5b	NP_001100647.1
Canis lupus familiaris (dog)	KDM5B	XP_005622266.1
Gallus gallus (chicken)	KDM5B	NP_001026200.1
Danio rerio (zebrafish)	kdm5bb	NP_001002166.1
Xenopus tropicalis (tropical clawed frog)	kdm5b	XP_004910700.1
Mus musculus (house mouse)	Kdm5b	NP_690855.2
Caenorhabditis elegans (roundworm)	rbr-2	NP_502032.2
Pan troglodytes (chimpanzee)	KDM5B	XP_514104.3
Homo sapiens (human)	KDM5B	NP_006609.3
Macaca mulatta (Rhesus monkey)	LOC706437	XP_002808303.1

Gene Ontology

The following kdm5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03747	XM_002939638.5 Latest version!	Xenopus tropicalis lysine demethylase 5B (kdm5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa42705	XM_018091505.2 Latest version!	Xenopus tropicalis lysine demethylase 5B (kdm5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa42705	XM_018091505.1	Xenopus tropicalis lysine (K)-specific demethylase 5B (kdm5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa03747	XM_002939638.4	Xenopus tropicalis lysine (K)-specific demethylase 5B (kdm5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kdm5b ( XM_002939638.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939684.2 Xenopus tropicalis lysine demethylase 5B (kdm5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03747
Clone ID Related Accession (Same CDS sequence)	XM_002939638.5 , XM_002939638.4
Accession Version	XM_002939638.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939638.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTCG TGCCCCCGCC GGAGTGCCCA GTTTTCGAGC CCACCTGGGA GGAATTCGCT 
GACCCCTTTG CGTTTATTAA TAAAATCCGA CCGATTGCTG AACGGAGTGG GATCTGCAAG 
GTCCGGCCGC CTCCGGACTG GCAGCCACCA TTTGCTTGTG ATGTGGATAA ACTGCACTTT 
ACTCCAAGGA TCCAAAGACT AAATGAGCTT GAGGCTCAGA CTAGAGTGAA GCTAAATTTC 
CTAGACCAGA TTGCTAAATT TTGGGAGTTG CAGGGATGTA CTCTGAAAAT TCCTCACGTG 
GAGCGAAAGA TTCTAGATTT ATTTCAGCTG AACAAGTTGG TGGCTGAAGA TGGCGGATTT 
GATTTAGTTT GTAGAGAAAG AAAATGGACC AAGATAGCTA CAAAAATGGG ATTTTCACTA 
GGAAAAGCCG TGGGTTCCCA TATTCGAGGC CATTATGAGC GAATTCTTCA CCCTTACAAC 
TTGTTCCAAT CTGGGACCAA CTTACTGGCT CCTGTGTCTT GCTCGAAGCA TCCAGGGACC 
ACAGACAATA ATTCTTATGA TAAGAAGCCA TTTCTTGCAC CTGATCTCAA GGACAAGGAG 
TACAAACCAC ATGACATCCC ACAGCGACAA TCTGTCCAGC CCCCAGAAAA CTGCACTCCT 
GCCAGAAGGG CCAAACGGAT GAAATCTGAG GTTGTTACCA TTAAAACTGA ACCAGTGGAA 
GTTCCAGAAC TAAGACCACG TGCCCTTCGT CGTAGAATGG GATACACCCC AACTAAGTGT 
GAAAAGGAGG ACAGTTTCCT TATAGTAAAG AAAGAGATAG TGGAACCAGT TATTGAGAAG 
CCCAAAGCAC GGAATAAAAA ATGTAGAAGC CAGGTGGATT TGTATGCTTG TCTTGTGTGT 
GGCAGTGGAA GTGATGAGGA TCGACTTCTG CTGTGTGATG GTTGTGATGA TAGTTACCAC 
ACTTTCTGCC TTATTCCTCC ACTCCAAGAT GTTCCCAAGG GAGACTGGAG ATGCCCTAAA 
TGCCTAGCAC AGGAGTGTAG TAAACCACAG GAAGCATTTG GGTTTGAACA GGCATCCAGA 
GATTACACCC TACGCATGTT TGGAGAGATG GCTGATAACT TCAAATCAGA TTACTTTAAC 
ATGCCAGTGC ATATGGTTCC CACAGAACTG GTGGAAAAAG AGTTCTGGCG CTTGGTGAGC 
ACCATAGAGG AGGATGTGAC AGTAGAATAT GGGGCTGACA TAGCTTCTAA GGAATTTGGA 
AGTGGCTTTC CTGTGCGTGA TGGTAAATTC CGTGTTAAAC CAGAAGATGA GGTATATCTG 
AATTGCGGGT GGAATCTAAA TAACATGCCT ATTATGCAGC CTTCTGTATT GGCACATGTC 
ACAGCAGATA TATGTGGCAT GAAATTACCC TGGCTTTATG TGGGCATGTG TTTCTCATCC 
TTTTGCTGGC ACATTGAGGA CCACTGGAGC TACTCTATTA ATTATCTGCA TTGGGGTGAG 
CCAAAAACTT GGTATGGAGC ACCTGGATAT GCTGCAGAAC AGCTGGAGGA GGTCATGAAA 
AAATTGGCGC CAGAATTATT CATTGCACAG CCTGACCTCT TGCATCAGCT AGTGACCATA 
ATGAATCCAA ATACACTAAT GGCTCATGGT GTTCCGATTT ATCGAACCAA TCAGTGTGCT 
GGAGAATTTG TGATCACTTT TCCACGTGCA TATCATAGTG GCTTTAATCA AGGCTTTAAC 
TTTGCAGAAG CCGTCAACTT CTGCACTGTT GATTGGCTTC CTCTTGGCCG TCAGTGCATT 
GAACATTACA GAGGGCTAAA CCGATACTGT GTATTTTCCC ATGATGAGAT GATCTGTAAA 
ATGGCCATTA AGGCAGATAA ACTTGATGTT GTACTAGCAT CTTCTGTACA GAAAGATATG 
GCTAGCATGA TAGACGAGGA GAGAGCCCTG CGTGAAGCTG TCCGACAGAT GGGGGTTTTG 
AAGTCAGAAA AGATGGACCT TGAACTCCTA GCAGATGATG ATCGGCAGTG TACAAAGTGT 
AAGACCACCT GTTTCATATC AGCAGTGTTC TGTTCGTGTA GCCCTGGGGC CCTTGTATGT 
CTTCATCATG TGGAGGATCT CTGTGATTGT CCAGTCTACA AGTACACATT GGGGTATCGT 
TACACTGTTG ATGATTTATA TCCCATGATG AACGCTGTTA AGCTGCGAGC AGAGTCATAT 
GACAGTTGGG CAACAGAAGT CATTGATGCT CTCAAAACTT CAGGCAATAA ACAAAGTCTC 
TCCTGCCTGA AGTTATTAAT TCAGGAATCC GAGGAAAAGA AATTTCCAGA AAATGATCTT 
TTACGCCACC TACGGGTTGT TGTACAGGAT GCTGAGAAAT GTGGAACATT GGCGCAACAG 
CTACTTAATG GGAAGCGTCA TACCAGGTTC AGGTCAGGTG GTGGGAAGTC GGCAAACCAG 
CTAACTGTTG CCGAACTCAG GTCTTTTGTA AAGCAGCTTC ACTCTCTGCC CTGTATTATC 
ACCCAGACAC CTCAGCTTAA GGATCTGCTT TGTCGTGTGG AAGAATTCCA GTTACAAAGC 
CAAAGACTTC TAACAGCAGA AATGCCACAA GCTTGTGAAC TTCAAGATCT GCTTGACTCT 
AGTTTTCAGT TTGACTTGGA CTTGCCACTG GTAGATATTC GGGAACGCCT TGAACAGGCT 
AGATGGCTAG AGGAGCAGCA TCAGGCATGC AAGGATCCTA GGGCACTTAC ATTGGATGTC 
ATGAGGAGAC TTATAGATTT GGGTGTGGGT CTCTCTCCTC ACCCTACTGT AGAAAAGGCA 
ATGGCCAAAC TTCAGGAGCT GCTTACTATG TCTGAACATT GGGATGATCG GTGTAGAAGC 
CTTCTCAAAT CCAGGCCCCG GCAACCACTT GATTCCTTAA CATCAGCTTT AAGAGAAGTG 
GAAAAAATCC CAGCTTTTTT GCCTATTGTG GAATCCTTAA GAGATGCTGT ACAAAGGGCT 
CATGAATGGT TGCAGGAAGT GGAGACTGTA CAGGCTGGGC CCAGGGTTCC TGTGTTGGAC 
ACATTGGTGG AACTTGTCGC CCATGGACGG GCAATTCCTG TTCATCTGGC ACCTCTGCCA 
AGGCTAGAGG CATTAGTGGG AGAAGTCCAT GCTTGGACTG AATGTGCAGC CAACACTTTT 
TTAACAGCCA ACTCCCCATA CACACTGCTT GAGGTCCTTT GCCCTCTTTG TGATGTTGGT 
ACCTCTGGCT TTAAAAGGAA AACAAAGAAA CTTAAAGAGC AGGTGTCCTT AAGTGGAAAG 
AAAAAGTATA TGAAGCCAGA TGGTCTGCAG GAACTGGAAA AAGCATTAAG TGAAAGCAGA 
GACACTGCCA CAGCGATGGC AGCCTTAGGA GAAGCCCGAC TACGTGAGAT GGAGGCCTTG 
CATGCTTTCA GGATTTCTAA TGAGAACAGA CTTCTATCCC ATGAGGAACA GAGTGACTGG 
AAGGTGTGCG TCTGCCAGGG AGAACCAATG GCTCCTATGA TCCAGTGTGA GCTGTGCAGA 
AGCTGCTTTC ACGCTGGTTG TATTACAGCT CCTCGACATG GAGCTCAGGT CTGGCTATGT 
CCTCAGTGCC ACCGCTCTGA AAAACCTCCT TTGGAGAAGA TCCTGCCTCT GTTAGCTTCC 
CTGCAACGTC TGCGTGTCCG TCTGCCAGAA GGAGATGCCT TACGTTACTT GATTGAAAGG 
ACAGTCAGTT GGCAACATCG CGCACAACAT ATGTTGTCTT CAAACGACCT GAAAATGGTG 
CAAGAGAAAA TTAGCTCGAG CCAGTTATAC AACAGGTGGC AAGCAACAGC CGGGCATTTG 
ACAGAGACCA ATGATGTATC GCAGAGTGTA GGGGCCACAT CATTCTCTCT ACCTCATGAG 
TGGGACAACA GAAGCATTTA TATCCGATCC CCATTTTCTC CTGGACGCAA CTGCATCCCA 
CTGAAAAGTC TGAATGCTGA ATTAGATGAA TTGTTAATGG AAGCTCAGCT TTTGCAAGTT 
TCACTTCCTG AGGTCCATGA GCTCTATCAT GCACTTCTGA CAGAGCCTTC TTCCACACAA 
GCCCCAGAAT GCAGCTTACC CAATGTTACC CATAGCAGGG AAAAAGGACC TGTGAGACAG 
CAGAGAGCAG ATCGAAGCCC AGTATTAACA GAGGCTGTAA ACTGCATACG GAAAGAAAGA 
TCAGATAGTA CAGACCAAAA GTTGAAGCGC CGTCGTGAAG GAAGAATGAG AAAGACTCCT 
ACTTTCCTCA AAAAGAGGGT TAAAGTAGCT AAACCAAAAG ACTTATGTGG CAGAGAGCTA 
CTTCCTGAGG AAAGCAGAGC AAGTGATTCC CAATCCTCCT TTACATCAGA TGAGTCTGAT 
GATGACGATG CATTTTGCCC TGCAGACAGA TGTATGGAAC CTGAAGGTGA TGAGGTTGAC 
TGGGTACAGT GTGATGGGAA TTGCAATCGT TGGTTTCATC AGGTTTGTGT TGGTCTTTCT 
GCGGAAACAG CTGAAAAGGA GGATTACATT TGTACACACT GTAAGGAGAT GACTTGTGCA 
CGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939684.2
CDS	375..4943
Translation	MEFVPPPECPVFEPTWEEFADPFAFINKIRPIAERSGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWELQGCTLKIPHVERKILDLFQLNKLVAEDGGFDLVCRERKWTKIATKMGFSLGKAVGSHIRGHYERILHPYNLFQSGTNLLAPVSCSKHPGTTDNNSYDKKPFLAPDLKDKEYKPHDIPQRQSVQPPENCTPARRAKRMKSEVVTIKTEPVEVPELRPRALRRRMGYTPTKCEKEDSFLIVKKEIVEPVIEKPKARNKKCRSQVDLYACLVCGSGSDEDRLLLCDGCDDSYHTFCLIPPLQDVPKGDWRCPKCLAQECSKPQEAFGFEQASRDYTLRMFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKFRVKPEDEVYLNCGWNLNNMPIMQPSVLAHVTADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGYAAEQLEEVMKKLAPELFIAQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCIEHYRGLNRYCVFSHDEMICKMAIKADKLDVVLASSVQKDMASMIDEERALREAVRQMGVLKSEKMDLELLADDDRQCTKCKTTCFISAVFCSCSPGALVCLHHVEDLCDCPVYKYTLGYRYTVDDLYPMMNAVKLRAESYDSWATEVIDALKTSGNKQSLSCLKLLIQESEEKKFPENDLLRHLRVVVQDAEKCGTLAQQLLNGKRHTRFRSGGGKSANQLTVAELRSFVKQLHSLPCIITQTPQLKDLLCRVEEFQLQSQRLLTAEMPQACELQDLLDSSFQFDLDLPLVDIRERLEQARWLEEQHQACKDPRALTLDVMRRLIDLGVGLSPHPTVEKAMAKLQELLTMSEHWDDRCRSLLKSRPRQPLDSLTSALREVEKIPAFLPIVESLRDAVQRAHEWLQEVETVQAGPRVPVLDTLVELVAHGRAIPVHLAPLPRLEALVGEVHAWTECAANTFLTANSPYTLLEVLCPLCDVGTSGFKRKTKKLKEQVSLSGKKKYMKPDGLQELEKALSESRDTATAMAALGEARLREMEALHAFRISNENRLLSHEEQSDWKVCVCQGEPMAPMIQCELCRSCFHAGCITAPRHGAQVWLCPQCHRSEKPPLEKILPLLASLQRLRVRLPEGDALRYLIERTVSWQHRAQHMLSSNDLKMVQEKISSSQLYNRWQATAGHLTETNDVSQSVGATSFSLPHEWDNRSIYIRSPFSPGRNCIPLKSLNAELDELLMEAQLLQVSLPEVHELYHALLTEPSSTQAPECSLPNVTHSREKGPVRQQRADRSPVLTEAVNCIRKERSDSTDQKLKRRREGRMRKTPTFLKKRVKVAKPKDLCGRELLPEESRASDSQSSFTSDESDDDDAFCPADRCMEPEGDEVDWVQCDGNCNRWFHQVCVGLSAETAEKEDYICTHCKEMTCARK

Target ORF information:

RefSeq Version	XM_002939638.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine demethylase 5B (kdm5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939638.5

ORF Insert Sequence:

ATGGAGTTCG TGCCCCCGCC GGAGTGCCCA GTTTTCGAGC CCACCTGGGA GGAATTCGCT 
GACCCCTTTG CGTTTATTAA TAAAATCCGA CCGATTGCTG AACGGAGTGG GATCTGCAAG 
GTCCGGCCGC CTCCGGACTG GCAGCCACCA TTTGCTTGTG ATGTGGATAA ACTGCACTTT 
ACTCCAAGGA TCCAAAGACT AAATGAGCTT GAGGCTCAGA CTAGAGTGAA GCTAAATTTC 
CTAGACCAGA TTGCTAAATT TTGGGAGTTG CAGGGATGTA CTCTGAAAAT TCCTCACGTG 
GAGCGAAAGA TTCTAGATTT ATTTCAGCTG AACAAGTTGG TGGCTGAAGA TGGCGGATTT 
GATTTAGTTT GTAGAGAAAG AAAATGGACC AAGATAGCTA CAAAAATGGG ATTTTCACTA 
GGAAAAGCCG TGGGTTCCCA TATTCGAGGC CATTATGAGC GAATTCTTCA CCCTTACAAC 
TTGTTCCAAT CTGGGACCAA CTTACTGGCT CCTGTGTCTT GCTCGAAGCA TCCAGGGACC 
ACAGACAATA ATTCTTATGA TAAGAAGCCA TTTCTTGCAC CTGATCTCAA GGACAAGGAG 
TACAAACCAC ATGACATCCC ACAGCGACAA TCTGTCCAGC CCCCAGAAAA CTGCACTCCT 
GCCAGAAGGG CCAAACGGAT GAAATCTGAG GTTGTTACCA TTAAAACTGA ACCAGTGGAA 
GTTCCAGAAC TAAGACCACG TGCCCTTCGT CGTAGAATGG GATACACCCC AACTAAGTGT 
GAAAAGGAGG ACAGTTTCCT TATAGTAAAG AAAGAGATAG TGGAACCAGT TATTGAGAAG 
CCCAAAGCAC GGAATAAAAA ATGTAGAAGC CAGGTGGATT TGTATGCTTG TCTTGTGTGT 
GGCAGTGGAA GTGATGAGGA TCGACTTCTG CTGTGTGATG GTTGTGATGA TAGTTACCAC 
ACTTTCTGCC TTATTCCTCC ACTCCAAGAT GTTCCCAAGG GAGACTGGAG ATGCCCTAAA 
TGCCTAGCAC AGGAGTGTAG TAAACCACAG GAAGCATTTG GGTTTGAACA GGCATCCAGA 
GATTACACCC TACGCATGTT TGGAGAGATG GCTGATAACT TCAAATCAGA TTACTTTAAC 
ATGCCAGTGC ATATGGTTCC CACAGAACTG GTGGAAAAAG AGTTCTGGCG CTTGGTGAGC 
ACCATAGAGG AGGATGTGAC AGTAGAATAT GGGGCTGACA TAGCTTCTAA GGAATTTGGA 
AGTGGCTTTC CTGTGCGTGA TGGTAAATTC CGTGTTAAAC CAGAAGATGA GGTATATCTG 
AATTGCGGGT GGAATCTAAA TAACATGCCT ATTATGCAGC CTTCTGTATT GGCACATGTC 
ACAGCAGATA TATGTGGCAT GAAATTACCC TGGCTTTATG TGGGCATGTG TTTCTCATCC 
TTTTGCTGGC ACATTGAGGA CCACTGGAGC TACTCTATTA ATTATCTGCA TTGGGGTGAG 
CCAAAAACTT GGTATGGAGC ACCTGGATAT GCTGCAGAAC AGCTGGAGGA GGTCATGAAA 
AAATTGGCGC CAGAATTATT CATTGCACAG CCTGACCTCT TGCATCAGCT AGTGACCATA 
ATGAATCCAA ATACACTAAT GGCTCATGGT GTTCCGATTT ATCGAACCAA TCAGTGTGCT 
GGAGAATTTG TGATCACTTT TCCACGTGCA TATCATAGTG GCTTTAATCA AGGCTTTAAC 
TTTGCAGAAG CCGTCAACTT CTGCACTGTT GATTGGCTTC CTCTTGGCCG TCAGTGCATT 
GAACATTACA GAGGGCTAAA CCGATACTGT GTATTTTCCC ATGATGAGAT GATCTGTAAA 
ATGGCCATTA AGGCAGATAA ACTTGATGTT GTACTAGCAT CTTCTGTACA GAAAGATATG 
GCTAGCATGA TAGACGAGGA GAGAGCCCTG CGTGAAGCTG TCCGACAGAT GGGGGTTTTG 
AAGTCAGAAA AGATGGACCT TGAACTCCTA GCAGATGATG ATCGGCAGTG TACAAAGTGT 
AAGACCACCT GTTTCATATC AGCAGTGTTC TGTTCGTGTA GCCCTGGGGC CCTTGTATGT 
CTTCATCATG TGGAGGATCT CTGTGATTGT CCAGTCTACA AGTACACATT GGGGTATCGT 
TACACTGTTG ATGATTTATA TCCCATGATG AACGCTGTTA AGCTGCGAGC AGAGTCATAT 
GACAGTTGGG CAACAGAAGT CATTGATGCT CTCAAAACTT CAGGCAATAA ACAAAGTCTC 
TCCTGCCTGA AGTTATTAAT TCAGGAATCC GAGGAAAAGA AATTTCCAGA AAATGATCTT 
TTACGCCACC TACGGGTTGT TGTACAGGAT GCTGAGAAAT GTGGAACATT GGCGCAACAG 
CTACTTAATG GGAAGCGTCA TACCAGGTTC AGGTCAGGTG GTGGGAAGTC GGCAAACCAG 
CTAACTGTTG CCGAACTCAG GTCTTTTGTA AAGCAGCTTC ACTCTCTGCC CTGTATTATC 
ACCCAGACAC CTCAGCTTAA GGATCTGCTT TGTCGTGTGG AAGAATTCCA GTTACAAAGC 
CAAAGACTTC TAACAGCAGA AATGCCACAA GCTTGTGAAC TTCAAGATCT GCTTGACTCT 
AGTTTTCAGT TTGACTTGGA CTTGCCACTG GTAGATATTC GGGAACGCCT TGAACAGGCT 
AGATGGCTAG AGGAGCAGCA TCAGGCATGC AAGGATCCTA GGGCACTTAC ATTGGATGTC 
ATGAGGAGAC TTATAGATTT GGGTGTGGGT CTCTCTCCTC ACCCTACTGT AGAAAAGGCA 
ATGGCCAAAC TTCAGGAGCT GCTTACTATG TCTGAACATT GGGATGATCG GTGTAGAAGC 
CTTCTCAAAT CCAGGCCCCG GCAACCACTT GATTCCTTAA CATCAGCTTT AAGAGAAGTG 
GAAAAAATCC CAGCTTTTTT GCCTATTGTG GAATCCTTAA GAGATGCTGT ACAAAGGGCT 
CATGAATGGT TGCAGGAAGT GGAGACTGTA CAGGCTGGGC CCAGGGTTCC TGTGTTGGAC 
ACATTGGTGG AACTTGTCGC CCATGGACGG GCAATTCCTG TTCATCTGGC ACCTCTGCCA 
AGGCTAGAGG CATTAGTGGG AGAAGTCCAT GCTTGGACTG AATGTGCAGC CAACACTTTT 
TTAACAGCCA ACTCCCCATA CACACTGCTT GAGGTCCTTT GCCCTCTTTG TGATGTTGGT 
ACCTCTGGCT TTAAAAGGAA AACAAAGAAA CTTAAAGAGC AGGTGTCCTT AAGTGGAAAG 
AAAAAGTATA TGAAGCCAGA TGGTCTGCAG GAACTGGAAA AAGCATTAAG TGAAAGCAGA 
GACACTGCCA CAGCGATGGC AGCCTTAGGA GAAGCCCGAC TACGTGAGAT GGAGGCCTTG 
CATGCTTTCA GGATTTCTAA TGAGAACAGA CTTCTATCCC ATGAGGAACA GAGTGACTGG 
AAGGTGTGCG TCTGCCAGGG AGAACCAATG GCTCCTATGA TCCAGTGTGA GCTGTGCAGA 
AGCTGCTTTC ACGCTGGTTG TATTACAGCT CCTCGACATG GAGCTCAGGT CTGGCTATGT 
CCTCAGTGCC ACCGCTCTGA AAAACCTCCT TTGGAGAAGA TCCTGCCTCT GTTAGCTTCC 
CTGCAACGTC TGCGTGTCCG TCTGCCAGAA GGAGATGCCT TACGTTACTT GATTGAAAGG 
ACAGTCAGTT GGCAACATCG CGCACAACAT ATGTTGTCTT CAAACGACCT GAAAATGGTG 
CAAGAGAAAA TTAGCTCGAG CCAGTTATAC AACAGGTGGC AAGCAACAGC CGGGCATTTG 
ACAGAGACCA ATGATGTATC GCAGAGTGTA GGGGCCACAT CATTCTCTCT ACCTCATGAG 
TGGGACAACA GAAGCATTTA TATCCGATCC CCATTTTCTC CTGGACGCAA CTGCATCCCA 
CTGAAAAGTC TGAATGCTGA ATTAGATGAA TTGTTAATGG AAGCTCAGCT TTTGCAAGTT 
TCACTTCCTG AGGTCCATGA GCTCTATCAT GCACTTCTGA CAGAGCCTTC TTCCACACAA 
GCCCCAGAAT GCAGCTTACC CAATGTTACC CATAGCAGGG AAAAAGGACC TGTGAGACAG 
CAGAGAGCAG ATCGAAGCCC AGTATTAACA GAGGCTGTAA ACTGCATACG GAAAGAAAGA 
TCAGATAGTA CAGACCAAAA GTTGAAGCGC CGTCGTGAAG GAAGAATGAG AAAGACTCCT 
ACTTTCCTCA AAAAGAGGGT TAAAGTAGCT AAACCAAAAG ACTTATGTGG CAGAGAGCTA 
CTTCCTGAGG AAAGCAGAGC AAGTGATTCC CAATCCTCCT TTACATCAGA TGAGTCTGAT 
GATGACGATG CATTTTGCCC TGCAGACAGA TGTATGGAAC CTGAAGGTGA TGAGGTTGAC 
TGGGTACAGT GTGATGGGAA TTGCAATCGT TGGTTTCATC AGGTTTGTGT TGGTCTTTCT 
GCGGAAACAG CTGAAAAGGA GGATTACATT TGTACACACT GTAAGGAGAT GACTTGTGCA 
CGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5b ( XM_002939638.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939684.2 Xenopus tropicalis lysine (K)-specific demethylase 5B (kdm5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03747
Clone ID Related Accession (Same CDS sequence)	XM_002939638.5 , XM_002939638.4
Accession Version	XM_002939638.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939638.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTCG TGCCCCCGCC GGAGTGCCCA GTTTTCGAGC CCACCTGGGA GGAATTCGCT 
GACCCCTTTG CGTTTATTAA TAAAATCCGA CCGATTGCTG AACGGAGTGG GATCTGCAAG 
GTCCGGCCGC CTCCGGACTG GCAGCCACCA TTTGCTTGTG ATGTGGATAA ACTGCACTTT 
ACTCCAAGGA TCCAAAGACT AAATGAGCTT GAGGCTCAGA CTAGAGTGAA GCTAAATTTC 
CTAGACCAGA TTGCTAAATT TTGGGAGTTG CAGGGATGTA CTCTGAAAAT TCCTCACGTG 
GAGCGAAAGA TTCTAGATTT ATTTCAGCTG AACAAGTTGG TGGCTGAAGA TGGCGGATTT 
GATTTAGTTT GTAGAGAAAG AAAATGGACC AAGATAGCTA CAAAAATGGG ATTTTCACTA 
GGAAAAGCCG TGGGTTCCCA TATTCGAGGC CATTATGAGC GAATTCTTCA CCCTTACAAC 
TTGTTCCAAT CTGGGACCAA CTTACTGGCT CCTGTGTCTT GCTCGAAGCA TCCAGGGACC 
ACAGACAATA ATTCTTATGA TAAGAAGCCA TTTCTTGCAC CTGATCTCAA GGACAAGGAG 
TACAAACCAC ATGACATCCC ACAGCGACAA TCTGTCCAGC CCCCAGAAAA CTGCACTCCT 
GCCAGAAGGG CCAAACGGAT GAAATCTGAG GTTGTTACCA TTAAAACTGA ACCAGTGGAA 
GTTCCAGAAC TAAGACCACG TGCCCTTCGT CGTAGAATGG GATACACCCC AACTAAGTGT 
GAAAAGGAGG ACAGTTTCCT TATAGTAAAG AAAGAGATAG TGGAACCAGT TATTGAGAAG 
CCCAAAGCAC GGAATAAAAA ATGTAGAAGC CAGGTGGATT TGTATGCTTG TCTTGTGTGT 
GGCAGTGGAA GTGATGAGGA TCGACTTCTG CTGTGTGATG GTTGTGATGA TAGTTACCAC 
ACTTTCTGCC TTATTCCTCC ACTCCAAGAT GTTCCCAAGG GAGACTGGAG ATGCCCTAAA 
TGCCTAGCAC AGGAGTGTAG TAAACCACAG GAAGCATTTG GGTTTGAACA GGCATCCAGA 
GATTACACCC TACGCATGTT TGGAGAGATG GCTGATAACT TCAAATCAGA TTACTTTAAC 
ATGCCAGTGC ATATGGTTCC CACAGAACTG GTGGAAAAAG AGTTCTGGCG CTTGGTGAGC 
ACCATAGAGG AGGATGTGAC AGTAGAATAT GGGGCTGACA TAGCTTCTAA GGAATTTGGA 
AGTGGCTTTC CTGTGCGTGA TGGTAAATTC CGTGTTAAAC CAGAAGATGA GGTATATCTG 
AATTGCGGGT GGAATCTAAA TAACATGCCT ATTATGCAGC CTTCTGTATT GGCACATGTC 
ACAGCAGATA TATGTGGCAT GAAATTACCC TGGCTTTATG TGGGCATGTG TTTCTCATCC 
TTTTGCTGGC ACATTGAGGA CCACTGGAGC TACTCTATTA ATTATCTGCA TTGGGGTGAG 
CCAAAAACTT GGTATGGAGC ACCTGGATAT GCTGCAGAAC AGCTGGAGGA GGTCATGAAA 
AAATTGGCGC CAGAATTATT CATTGCACAG CCTGACCTCT TGCATCAGCT AGTGACCATA 
ATGAATCCAA ATACACTAAT GGCTCATGGT GTTCCGATTT ATCGAACCAA TCAGTGTGCT 
GGAGAATTTG TGATCACTTT TCCACGTGCA TATCATAGTG GCTTTAATCA AGGCTTTAAC 
TTTGCAGAAG CCGTCAACTT CTGCACTGTT GATTGGCTTC CTCTTGGCCG TCAGTGCATT 
GAACATTACA GAGGGCTAAA CCGATACTGT GTATTTTCCC ATGATGAGAT GATCTGTAAA 
ATGGCCATTA AGGCAGATAA ACTTGATGTT GTACTAGCAT CTTCTGTACA GAAAGATATG 
GCTAGCATGA TAGACGAGGA GAGAGCCCTG CGTGAAGCTG TCCGACAGAT GGGGGTTTTG 
AAGTCAGAAA AGATGGACCT TGAACTCCTA GCAGATGATG ATCGGCAGTG TACAAAGTGT 
AAGACCACCT GTTTCATATC AGCAGTGTTC TGTTCGTGTA GCCCTGGGGC CCTTGTATGT 
CTTCATCATG TGGAGGATCT CTGTGATTGT CCAGTCTACA AGTACACATT GGGGTATCGT 
TACACTGTTG ATGATTTATA TCCCATGATG AACGCTGTTA AGCTGCGAGC AGAGTCATAT 
GACAGTTGGG CAACAGAAGT CATTGATGCT CTCAAAACTT CAGGCAATAA ACAAAGTCTC 
TCCTGCCTGA AGTTATTAAT TCAGGAATCC GAGGAAAAGA AATTTCCAGA AAATGATCTT 
TTACGCCACC TACGGGTTGT TGTACAGGAT GCTGAGAAAT GTGGAACATT GGCGCAACAG 
CTACTTAATG GGAAGCGTCA TACCAGGTTC AGGTCAGGTG GTGGGAAGTC GGCAAACCAG 
CTAACTGTTG CCGAACTCAG GTCTTTTGTA AAGCAGCTTC ACTCTCTGCC CTGTATTATC 
ACCCAGACAC CTCAGCTTAA GGATCTGCTT TGTCGTGTGG AAGAATTCCA GTTACAAAGC 
CAAAGACTTC TAACAGCAGA AATGCCACAA GCTTGTGAAC TTCAAGATCT GCTTGACTCT 
AGTTTTCAGT TTGACTTGGA CTTGCCACTG GTAGATATTC GGGAACGCCT TGAACAGGCT 
AGATGGCTAG AGGAGCAGCA TCAGGCATGC AAGGATCCTA GGGCACTTAC ATTGGATGTC 
ATGAGGAGAC TTATAGATTT GGGTGTGGGT CTCTCTCCTC ACCCTACTGT AGAAAAGGCA 
ATGGCCAAAC TTCAGGAGCT GCTTACTATG TCTGAACATT GGGATGATCG GTGTAGAAGC 
CTTCTCAAAT CCAGGCCCCG GCAACCACTT GATTCCTTAA CATCAGCTTT AAGAGAAGTG 
GAAAAAATCC CAGCTTTTTT GCCTATTGTG GAATCCTTAA GAGATGCTGT ACAAAGGGCT 
CATGAATGGT TGCAGGAAGT GGAGACTGTA CAGGCTGGGC CCAGGGTTCC TGTGTTGGAC 
ACATTGGTGG AACTTGTCGC CCATGGACGG GCAATTCCTG TTCATCTGGC ACCTCTGCCA 
AGGCTAGAGG CATTAGTGGG AGAAGTCCAT GCTTGGACTG AATGTGCAGC CAACACTTTT 
TTAACAGCCA ACTCCCCATA CACACTGCTT GAGGTCCTTT GCCCTCTTTG TGATGTTGGT 
ACCTCTGGCT TTAAAAGGAA AACAAAGAAA CTTAAAGAGC AGGTGTCCTT AAGTGGAAAG 
AAAAAGTATA TGAAGCCAGA TGGTCTGCAG GAACTGGAAA AAGCATTAAG TGAAAGCAGA 
GACACTGCCA CAGCGATGGC AGCCTTAGGA GAAGCCCGAC TACGTGAGAT GGAGGCCTTG 
CATGCTTTCA GGATTTCTAA TGAGAACAGA CTTCTATCCC ATGAGGAACA GAGTGACTGG 
AAGGTGTGCG TCTGCCAGGG AGAACCAATG GCTCCTATGA TCCAGTGTGA GCTGTGCAGA 
AGCTGCTTTC ACGCTGGTTG TATTACAGCT CCTCGACATG GAGCTCAGGT CTGGCTATGT 
CCTCAGTGCC ACCGCTCTGA AAAACCTCCT TTGGAGAAGA TCCTGCCTCT GTTAGCTTCC 
CTGCAACGTC TGCGTGTCCG TCTGCCAGAA GGAGATGCCT TACGTTACTT GATTGAAAGG 
ACAGTCAGTT GGCAACATCG CGCACAACAT ATGTTGTCTT CAAACGACCT GAAAATGGTG 
CAAGAGAAAA TTAGCTCGAG CCAGTTATAC AACAGGTGGC AAGCAACAGC CGGGCATTTG 
ACAGAGACCA ATGATGTATC GCAGAGTGTA GGGGCCACAT CATTCTCTCT ACCTCATGAG 
TGGGACAACA GAAGCATTTA TATCCGATCC CCATTTTCTC CTGGACGCAA CTGCATCCCA 
CTGAAAAGTC TGAATGCTGA ATTAGATGAA TTGTTAATGG AAGCTCAGCT TTTGCAAGTT 
TCACTTCCTG AGGTCCATGA GCTCTATCAT GCACTTCTGA CAGAGCCTTC TTCCACACAA 
GCCCCAGAAT GCAGCTTACC CAATGTTACC CATAGCAGGG AAAAAGGACC TGTGAGACAG 
CAGAGAGCAG ATCGAAGCCC AGTATTAACA GAGGCTGTAA ACTGCATACG GAAAGAAAGA 
TCAGATAGTA CAGACCAAAA GTTGAAGCGC CGTCGTGAAG GAAGAATGAG AAAGACTCCT 
ACTTTCCTCA AAAAGAGGGT TAAAGTAGCT AAACCAAAAG ACTTATGTGG CAGAGAGCTA 
CTTCCTGAGG AAAGCAGAGC AAGTGATTCC CAATCCTCCT TTACATCAGA TGAGTCTGAT 
GATGACGATG CATTTTGCCC TGCAGACAGA TGTATGGAAC CTGAAGGTGA TGAGGTTGAC 
TGGGTACAGT GTGATGGGAA TTGCAATCGT TGGTTTCATC AGGTTTGTGT TGGTCTTTCT 
GCGGAAACAG CTGAAAAGGA GGATTACATT TGTACACACT GTAAGGAGAT GACTTGTGCA 
CGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939684.2
CDS	715..5283
Translation	MEFVPPPECPVFEPTWEEFADPFAFINKIRPIAERSGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWELQGCTLKIPHVERKILDLFQLNKLVAEDGGFDLVCRERKWTKIATKMGFSLGKAVGSHIRGHYERILHPYNLFQSGTNLLAPVSCSKHPGTTDNNSYDKKPFLAPDLKDKEYKPHDIPQRQSVQPPENCTPARRAKRMKSEVVTIKTEPVEVPELRPRALRRRMGYTPTKCEKEDSFLIVKKEIVEPVIEKPKARNKKCRSQVDLYACLVCGSGSDEDRLLLCDGCDDSYHTFCLIPPLQDVPKGDWRCPKCLAQECSKPQEAFGFEQASRDYTLRMFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKFRVKPEDEVYLNCGWNLNNMPIMQPSVLAHVTADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGYAAEQLEEVMKKLAPELFIAQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCIEHYRGLNRYCVFSHDEMICKMAIKADKLDVVLASSVQKDMASMIDEERALREAVRQMGVLKSEKMDLELLADDDRQCTKCKTTCFISAVFCSCSPGALVCLHHVEDLCDCPVYKYTLGYRYTVDDLYPMMNAVKLRAESYDSWATEVIDALKTSGNKQSLSCLKLLIQESEEKKFPENDLLRHLRVVVQDAEKCGTLAQQLLNGKRHTRFRSGGGKSANQLTVAELRSFVKQLHSLPCIITQTPQLKDLLCRVEEFQLQSQRLLTAEMPQACELQDLLDSSFQFDLDLPLVDIRERLEQARWLEEQHQACKDPRALTLDVMRRLIDLGVGLSPHPTVEKAMAKLQELLTMSEHWDDRCRSLLKSRPRQPLDSLTSALREVEKIPAFLPIVESLRDAVQRAHEWLQEVETVQAGPRVPVLDTLVELVAHGRAIPVHLAPLPRLEALVGEVHAWTECAANTFLTANSPYTLLEVLCPLCDVGTSGFKRKTKKLKEQVSLSGKKKYMKPDGLQELEKALSESRDTATAMAALGEARLREMEALHAFRISNENRLLSHEEQSDWKVCVCQGEPMAPMIQCELCRSCFHAGCITAPRHGAQVWLCPQCHRSEKPPLEKILPLLASLQRLRVRLPEGDALRYLIERTVSWQHRAQHMLSSNDLKMVQEKISSSQLYNRWQATAGHLTETNDVSQSVGATSFSLPHEWDNRSIYIRSPFSPGRNCIPLKSLNAELDELLMEAQLLQVSLPEVHELYHALLTEPSSTQAPECSLPNVTHSREKGPVRQQRADRSPVLTEAVNCIRKERSDSTDQKLKRRREGRMRKTPTFLKKRVKVAKPKDLCGRELLPEESRASDSQSSFTSDESDDDDAFCPADRCMEPEGDEVDWVQCDGNCNRWFHQVCVGLSAETAEKEDYICTHCKEMTCARK

Target ORF information:

RefSeq Version	XM_002939638.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 5B (kdm5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939638.4

ORF Insert Sequence:

ATGGAGTTCG TGCCCCCGCC GGAGTGCCCA GTTTTCGAGC CCACCTGGGA GGAATTCGCT 
GACCCCTTTG CGTTTATTAA TAAAATCCGA CCGATTGCTG AACGGAGTGG GATCTGCAAG 
GTCCGGCCGC CTCCGGACTG GCAGCCACCA TTTGCTTGTG ATGTGGATAA ACTGCACTTT 
ACTCCAAGGA TCCAAAGACT AAATGAGCTT GAGGCTCAGA CTAGAGTGAA GCTAAATTTC 
CTAGACCAGA TTGCTAAATT TTGGGAGTTG CAGGGATGTA CTCTGAAAAT TCCTCACGTG 
GAGCGAAAGA TTCTAGATTT ATTTCAGCTG AACAAGTTGG TGGCTGAAGA TGGCGGATTT 
GATTTAGTTT GTAGAGAAAG AAAATGGACC AAGATAGCTA CAAAAATGGG ATTTTCACTA 
GGAAAAGCCG TGGGTTCCCA TATTCGAGGC CATTATGAGC GAATTCTTCA CCCTTACAAC 
TTGTTCCAAT CTGGGACCAA CTTACTGGCT CCTGTGTCTT GCTCGAAGCA TCCAGGGACC 
ACAGACAATA ATTCTTATGA TAAGAAGCCA TTTCTTGCAC CTGATCTCAA GGACAAGGAG 
TACAAACCAC ATGACATCCC ACAGCGACAA TCTGTCCAGC CCCCAGAAAA CTGCACTCCT 
GCCAGAAGGG CCAAACGGAT GAAATCTGAG GTTGTTACCA TTAAAACTGA ACCAGTGGAA 
GTTCCAGAAC TAAGACCACG TGCCCTTCGT CGTAGAATGG GATACACCCC AACTAAGTGT 
GAAAAGGAGG ACAGTTTCCT TATAGTAAAG AAAGAGATAG TGGAACCAGT TATTGAGAAG 
CCCAAAGCAC GGAATAAAAA ATGTAGAAGC CAGGTGGATT TGTATGCTTG TCTTGTGTGT 
GGCAGTGGAA GTGATGAGGA TCGACTTCTG CTGTGTGATG GTTGTGATGA TAGTTACCAC 
ACTTTCTGCC TTATTCCTCC ACTCCAAGAT GTTCCCAAGG GAGACTGGAG ATGCCCTAAA 
TGCCTAGCAC AGGAGTGTAG TAAACCACAG GAAGCATTTG GGTTTGAACA GGCATCCAGA 
GATTACACCC TACGCATGTT TGGAGAGATG GCTGATAACT TCAAATCAGA TTACTTTAAC 
ATGCCAGTGC ATATGGTTCC CACAGAACTG GTGGAAAAAG AGTTCTGGCG CTTGGTGAGC 
ACCATAGAGG AGGATGTGAC AGTAGAATAT GGGGCTGACA TAGCTTCTAA GGAATTTGGA 
AGTGGCTTTC CTGTGCGTGA TGGTAAATTC CGTGTTAAAC CAGAAGATGA GGTATATCTG 
AATTGCGGGT GGAATCTAAA TAACATGCCT ATTATGCAGC CTTCTGTATT GGCACATGTC 
ACAGCAGATA TATGTGGCAT GAAATTACCC TGGCTTTATG TGGGCATGTG TTTCTCATCC 
TTTTGCTGGC ACATTGAGGA CCACTGGAGC TACTCTATTA ATTATCTGCA TTGGGGTGAG 
CCAAAAACTT GGTATGGAGC ACCTGGATAT GCTGCAGAAC AGCTGGAGGA GGTCATGAAA 
AAATTGGCGC CAGAATTATT CATTGCACAG CCTGACCTCT TGCATCAGCT AGTGACCATA 
ATGAATCCAA ATACACTAAT GGCTCATGGT GTTCCGATTT ATCGAACCAA TCAGTGTGCT 
GGAGAATTTG TGATCACTTT TCCACGTGCA TATCATAGTG GCTTTAATCA AGGCTTTAAC 
TTTGCAGAAG CCGTCAACTT CTGCACTGTT GATTGGCTTC CTCTTGGCCG TCAGTGCATT 
GAACATTACA GAGGGCTAAA CCGATACTGT GTATTTTCCC ATGATGAGAT GATCTGTAAA 
ATGGCCATTA AGGCAGATAA ACTTGATGTT GTACTAGCAT CTTCTGTACA GAAAGATATG 
GCTAGCATGA TAGACGAGGA GAGAGCCCTG CGTGAAGCTG TCCGACAGAT GGGGGTTTTG 
AAGTCAGAAA AGATGGACCT TGAACTCCTA GCAGATGATG ATCGGCAGTG TACAAAGTGT 
AAGACCACCT GTTTCATATC AGCAGTGTTC TGTTCGTGTA GCCCTGGGGC CCTTGTATGT 
CTTCATCATG TGGAGGATCT CTGTGATTGT CCAGTCTACA AGTACACATT GGGGTATCGT 
TACACTGTTG ATGATTTATA TCCCATGATG AACGCTGTTA AGCTGCGAGC AGAGTCATAT 
GACAGTTGGG CAACAGAAGT CATTGATGCT CTCAAAACTT CAGGCAATAA ACAAAGTCTC 
TCCTGCCTGA AGTTATTAAT TCAGGAATCC GAGGAAAAGA AATTTCCAGA AAATGATCTT 
TTACGCCACC TACGGGTTGT TGTACAGGAT GCTGAGAAAT GTGGAACATT GGCGCAACAG 
CTACTTAATG GGAAGCGTCA TACCAGGTTC AGGTCAGGTG GTGGGAAGTC GGCAAACCAG 
CTAACTGTTG CCGAACTCAG GTCTTTTGTA AAGCAGCTTC ACTCTCTGCC CTGTATTATC 
ACCCAGACAC CTCAGCTTAA GGATCTGCTT TGTCGTGTGG AAGAATTCCA GTTACAAAGC 
CAAAGACTTC TAACAGCAGA AATGCCACAA GCTTGTGAAC TTCAAGATCT GCTTGACTCT 
AGTTTTCAGT TTGACTTGGA CTTGCCACTG GTAGATATTC GGGAACGCCT TGAACAGGCT 
AGATGGCTAG AGGAGCAGCA TCAGGCATGC AAGGATCCTA GGGCACTTAC ATTGGATGTC 
ATGAGGAGAC TTATAGATTT GGGTGTGGGT CTCTCTCCTC ACCCTACTGT AGAAAAGGCA 
ATGGCCAAAC TTCAGGAGCT GCTTACTATG TCTGAACATT GGGATGATCG GTGTAGAAGC 
CTTCTCAAAT CCAGGCCCCG GCAACCACTT GATTCCTTAA CATCAGCTTT AAGAGAAGTG 
GAAAAAATCC CAGCTTTTTT GCCTATTGTG GAATCCTTAA GAGATGCTGT ACAAAGGGCT 
CATGAATGGT TGCAGGAAGT GGAGACTGTA CAGGCTGGGC CCAGGGTTCC TGTGTTGGAC 
ACATTGGTGG AACTTGTCGC CCATGGACGG GCAATTCCTG TTCATCTGGC ACCTCTGCCA 
AGGCTAGAGG CATTAGTGGG AGAAGTCCAT GCTTGGACTG AATGTGCAGC CAACACTTTT 
TTAACAGCCA ACTCCCCATA CACACTGCTT GAGGTCCTTT GCCCTCTTTG TGATGTTGGT 
ACCTCTGGCT TTAAAAGGAA AACAAAGAAA CTTAAAGAGC AGGTGTCCTT AAGTGGAAAG 
AAAAAGTATA TGAAGCCAGA TGGTCTGCAG GAACTGGAAA AAGCATTAAG TGAAAGCAGA 
GACACTGCCA CAGCGATGGC AGCCTTAGGA GAAGCCCGAC TACGTGAGAT GGAGGCCTTG 
CATGCTTTCA GGATTTCTAA TGAGAACAGA CTTCTATCCC ATGAGGAACA GAGTGACTGG 
AAGGTGTGCG TCTGCCAGGG AGAACCAATG GCTCCTATGA TCCAGTGTGA GCTGTGCAGA 
AGCTGCTTTC ACGCTGGTTG TATTACAGCT CCTCGACATG GAGCTCAGGT CTGGCTATGT 
CCTCAGTGCC ACCGCTCTGA AAAACCTCCT TTGGAGAAGA TCCTGCCTCT GTTAGCTTCC 
CTGCAACGTC TGCGTGTCCG TCTGCCAGAA GGAGATGCCT TACGTTACTT GATTGAAAGG 
ACAGTCAGTT GGCAACATCG CGCACAACAT ATGTTGTCTT CAAACGACCT GAAAATGGTG 
CAAGAGAAAA TTAGCTCGAG CCAGTTATAC AACAGGTGGC AAGCAACAGC CGGGCATTTG 
ACAGAGACCA ATGATGTATC GCAGAGTGTA GGGGCCACAT CATTCTCTCT ACCTCATGAG 
TGGGACAACA GAAGCATTTA TATCCGATCC CCATTTTCTC CTGGACGCAA CTGCATCCCA 
CTGAAAAGTC TGAATGCTGA ATTAGATGAA TTGTTAATGG AAGCTCAGCT TTTGCAAGTT 
TCACTTCCTG AGGTCCATGA GCTCTATCAT GCACTTCTGA CAGAGCCTTC TTCCACACAA 
GCCCCAGAAT GCAGCTTACC CAATGTTACC CATAGCAGGG AAAAAGGACC TGTGAGACAG 
CAGAGAGCAG ATCGAAGCCC AGTATTAACA GAGGCTGTAA ACTGCATACG GAAAGAAAGA 
TCAGATAGTA CAGACCAAAA GTTGAAGCGC CGTCGTGAAG GAAGAATGAG AAAGACTCCT 
ACTTTCCTCA AAAAGAGGGT TAAAGTAGCT AAACCAAAAG ACTTATGTGG CAGAGAGCTA 
CTTCCTGAGG AAAGCAGAGC AAGTGATTCC CAATCCTCCT TTACATCAGA TGAGTCTGAT 
GATGACGATG CATTTTGCCC TGCAGACAGA TGTATGGAAC CTGAAGGTGA TGAGGTTGAC 
TGGGTACAGT GTGATGGGAA TTGCAATCGT TGGTTTCATC AGGTTTGTGT TGGTCTTTCT 
GCGGAAACAG CTGAAAAGGA GGATTACATT TGTACACACT GTAAGGAGAT GACTTGTGCA 
CGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5b ( XM_018091505.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946994.1 Xenopus tropicalis lysine demethylase 5B (kdm5b), transcript variant X1, mRNA.

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CloneID	OXa42705
Clone ID Related Accession (Same CDS sequence)	XM_018091505.2 , XM_018091505.1
Accession Version	XM_018091505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT CCGCTTCCCC TCTGTGCGTT TCATTATTCA GAATCCTACC TGCGGACCTC 
CAGTTGTTGT TAAAGTCCCA GCATCCCCTA ATTGTGTTGG GAATAGTAGG AGCTGGAGGC 
CCGCAGGTTG CCCTGTATTC ATGCTCCATC CATGTTAAGG ACTGGCAGCC ACCATTTGCT 
TGTGATGTGG ATAAACTGCA CTTTACTCCA AGGATCCAAA GACTAAATGA GCTTGAGGCT 
CAGACTAGAG TGAAGCTAAA TTTCCTAGAC CAGATTGCTA AATTTTGGGA GTTGCAGGGA 
TGTACTCTGA AAATTCCTCA CGTGGAGCGA AAGATTCTAG ATTTATTTCA GCTGAACAAG 
TTGGTGGCTG AAGATGGCGG ATTTGATTTA GTTTGTAGAG AAAGAAAATG GACCAAGATA 
GCTACAAAAA TGGGATTTTC ACTAGGAAAA GCCGTGGGTT CCCATATTCG AGGCCATTAT 
GAGCGAATTC TTCACCCTTA CAACTTGTTC CAATCTGGGA CCAACTTACT GGCTCCTGTG 
TCTTGCTCGA AGCATCCAGG GACCACAGAC AATAATTCTT ATGATAAGAA GCCATTTCTT 
GCACCTGATC TCAAGGACAA GGAGTACAAA CCACATGACA TCCCACAGCG ACAATCTGTC 
CAGCCCCCAG AAAACTGCAC TCCTGCCAGA AGGGCCAAAC GGATGAAATC TGAGGTTGTT 
ACCATTAAAA CTGAACCAGT GGAAGTTCCA GAACTAAGAC CACGTGCCCT TCGTCGTAGA 
ATGGGATACA CCCCAACTAA GTGTGAAAAG GAGGACAGTT TCCTTATAGT AAAGAAAGAG 
ATAGTGGAAC CAGTTATTGA GAAGCCCAAA GCACGGAATA AAAAATGTAG AAGCCAGGTG 
GATTTGTATG CTTGTCTTGT GTGTGGCAGT GGAAGTGATG AGGATCGACT TCTGCTGTGT 
GATGGTTGTG ATGATAGTTA CCACACTTTC TGCCTTATTC CTCCACTCCA AGATGTTCCC 
AAGGGAGACT GGAGATGCCC TAAATGCCTA GCACAGGAGT GTAGTAAACC ACAGGAAGCA 
TTTGGGTTTG AACAGGCATC CAGAGATTAC ACCCTACGCA TGTTTGGAGA GATGGCTGAT 
AACTTCAAAT CAGATTACTT TAACATGCCA GTGCATATGG TTCCCACAGA ACTGGTGGAA 
AAAGAGTTCT GGCGCTTGGT GAGCACCATA GAGGAGGATG TGACAGTAGA ATATGGGGCT 
GACATAGCTT CTAAGGAATT TGGAAGTGGC TTTCCTGTGC GTGATGGTAA ATTCCGTGTT 
AAACCAGAAG ATGAGGTATA TCTGAATTGC GGGTGGAATC TAAATAACAT GCCTATTATG 
CAGCCTTCTG TATTGGCACA TGTCACAGCA GATATATGTG GCATGAAATT ACCCTGGCTT 
TATGTGGGCA TGTGTTTCTC ATCCTTTTGC TGGCACATTG AGGACCACTG GAGCTACTCT 
ATTAATTATC TGCATTGGGG TGAGCCAAAA ACTTGGTATG GAGCACCTGG ATATGCTGCA 
GAACAGCTGG AGGAGGTCAT GAAAAAATTG GCGCCAGAAT TATTCATTGC ACAGCCTGAC 
CTCTTGCATC AGCTAGTGAC CATAATGAAT CCAAATACAC TAATGGCTCA TGGTGTTCCG 
ATTTATCGAA CCAATCAGTG TGCTGGAGAA TTTGTGATCA CTTTTCCACG TGCATATCAT 
AGTGGCTTTA ATCAAGGCTT TAACTTTGCA GAAGCCGTCA ACTTCTGCAC TGTTGATTGG 
CTTCCTCTTG GCCGTCAGTG CATTGAACAT TACAGAGGGC TAAACCGATA CTGTGTATTT 
TCCCATGATG AGATGATCTG TAAAATGGCC ATTAAGGCAG ATAAACTTGA TGTTGTACTA 
GCATCTTCTG TACAGAAAGA TATGGCTAGC ATGATAGACG AGGAGAGAGC CCTGCGTGAA 
GCTGTCCGAC AGATGGGGGT TTTGAAGTCA GAAAAGATGG ACCTTGAACT CCTAGCAGAT 
GATGATCGGC AGTGTACAAA GTGTAAGACC ACCTGTTTCA TATCAGCAGT GTTCTGTTCG 
TGTAGCCCTG GGGCCCTTGT ATGTCTTCAT CATGTGGAGG ATCTCTGTGA TTGTCCAGTC 
TACAAGTACA CATTGGGGTA TCGTTACACT GTTGATGATT TATATCCCAT GATGAACGCT 
GTTAAGCTGC GAGCAGAGTC ATATGACAGT TGGGCAACAG AAGTCATTGA TGCTCTCAAA 
ACTTCAGGCA ATAAACAAAG TCTCTCCTGC CTGAAGTTAT TAATTCAGGA ATCCGAGGAA 
AAGAAATTTC CAGAAAATGA TCTTTTACGC CACCTACGGG TTGTTGTACA GGATGCTGAG 
AAATGTGGAA CATTGGCGCA ACAGCTACTT AATGGGAAGC GTCATACCAG GTTCAGGTCA 
GGTGGTGGGA AGTCGGCAAA CCAGCTAACT GTTGCCGAAC TCAGGTCTTT TGTAAAGCAG 
CTTCACTCTC TGCCCTGTAT TATCACCCAG ACACCTCAGC TTAAGGATCT GCTTTGTCGT 
GTGGAAGAAT TCCAGTTACA AAGCCAAAGA CTTCTAACAG CAGAAATGCC ACAAGCTTGT 
GAACTTCAAG ATCTGCTTGA CTCTAGTTTT CAGTTTGACT TGGACTTGCC ACTGGTAGAT 
ATTCGGGAAC GCCTTGAACA GGCTAGATGG CTAGAGGAGC AGCATCAGGC ATGCAAGGAT 
CCTAGGGCAC TTACATTGGA TGTCATGAGG AGACTTATAG ATTTGGGTGT GGGTCTCTCT 
CCTCACCCTA CTGTAGAAAA GGCAATGGCC AAACTTCAGG AGCTGCTTAC TATGTCTGAA 
CATTGGGATG ATCGGTGTAG AAGCCTTCTC AAATCCAGGC CCCGGCAACC ACTTGATTCC 
TTAACATCAG CTTTAAGAGA AGTGGAAAAA ATCCCAGCTT TTTTGCCTAT TGTGGAATCC 
TTAAGAGATG CTGTACAAAG GGCTCATGAA TGGTTGCAGG AAGTGGAGAC TGTACAGGCT 
GGGCCCAGGG TTCCTGTGTT GGACACATTG GTGGAACTTG TCGCCCATGG ACGGGCAATT 
CCTGTTCATC TGGCACCTCT GCCAAGGCTA GAGGCATTAG TGGGAGAAGT CCATGCTTGG 
ACTGAATGTG CAGCCAACAC TTTTTTAACA GCCAACTCCC CATACACACT GCTTGAGGTC 
CTTTGCCCTC TTTGTGATGT TGGTACCTCT GGCTTTAAAA GGAAAACAAA GAAACTTAAA 
GAGCAGGTGT CCTTAAGTGG AAAGAAAAAG TATATGAAGC CAGATGGTCT GCAGGAACTG 
GAAAAAGCAT TAAGTGAAAG CAGAGACACT GCCACAGCGA TGGCAGCCTT AGGAGAAGCC 
CGACTACGTG AGATGGAGGC CTTGCATGCT TTCAGGATTT CTAATGAGAA CAGACTTCTA 
TCCCATGAGG AACAGAGTGA CTGGAAGGTG TGCGTCTGCC AGGGAGAACC AATGGCTCCT 
ATGATCCAGT GTGAGCTGTG CAGAAGCTGC TTTCACGCTG GTTGTATTAC AGCTCCTCGA 
CATGGAGCTC AGGTCTGGCT ATGTCCTCAG TGCCACCGCT CTGAAAAACC TCCTTTGGAG 
AAGATCCTGC CTCTGTTAGC TTCCCTGCAA CGTCTGCGTG TCCGTCTGCC AGAAGGAGAT 
GCCTTACGTT ACTTGATTGA AAGGACAGTC AGTTGGCAAC ATCGCGCACA ACATATGTTG 
TCTTCAAACG ACCTGAAAAT GGTGCAAGAG AAAATTAGCT CGAGCCAGTT ATACAACAGG 
TGGCAAGCAA CAGCCGGGCA TTTGACAGAG ACCAATGATG TATCGCAGAG TGTAGGGGCC 
ACATCATTCT CTCTACCTCA TGAGTGGGAC AACAGAAGCA TTTATATCCG ATCCCCATTT 
TCTCCTGGAC GCAACTGCAT CCCACTGAAA AGTCTGAATG CTGAATTAGA TGAATTGTTA 
ATGGAAGCTC AGCTTTTGCA AGTTTCACTT CCTGAGGTCC ATGAGCTCTA TCATGCACTT 
CTGACAGAGC CTTCTTCCAC ACAAGCCCCA GAATGCAGCT TACCCAATGT TACCCATAGC 
AGGGAAAAAG GACCTGTGAG ACAGCAGAGA GCAGATCGAA GCCCAGTATT AACAGAGGCT 
GTAAACTGCA TACGGAAAGA AAGATCAGAT AGTACAGACC AAAAGTTGAA GCGCCGTCGT 
GAAGGAAGAA TGAGAAAGAC TCCTACTTTC CTCAAAAAGA GGGTTAAAGT AGCTAAACCA 
AAAGACTTAT GTGGCAGAGA GCTACTTCCT GAGGAAAGCA GAGCAAGTGA TTCCCAATCC 
TCCTTTACAT CAGATGAGTC TGATGATGAC GATGCATTTT GCCCTGCAGA CAGATGTATG 
GAACCTGAAG GTGATGAGGT TGACTGGGTA CAGTGTGATG GGAATTGCAA TCGTTGGTTT 
CATCAGGTTT GTGTTGGTCT TTCTGCGGAA ACAGCTGAAA AGGAGGATTA CATTTGTACA 
CACTGTAAGG AGATGACTTG TGCACGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946994.1
CDS	18..4610
Translation	MDRSASPLCVSLFRILPADLQLLLKSQHPLIVLGIVGAGGPQVALYSCSIHVKDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWELQGCTLKIPHVERKILDLFQLNKLVAEDGGFDLVCRERKWTKIATKMGFSLGKAVGSHIRGHYERILHPYNLFQSGTNLLAPVSCSKHPGTTDNNSYDKKPFLAPDLKDKEYKPHDIPQRQSVQPPENCTPARRAKRMKSEVVTIKTEPVEVPELRPRALRRRMGYTPTKCEKEDSFLIVKKEIVEPVIEKPKARNKKCRSQVDLYACLVCGSGSDEDRLLLCDGCDDSYHTFCLIPPLQDVPKGDWRCPKCLAQECSKPQEAFGFEQASRDYTLRMFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKFRVKPEDEVYLNCGWNLNNMPIMQPSVLAHVTADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGYAAEQLEEVMKKLAPELFIAQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCIEHYRGLNRYCVFSHDEMICKMAIKADKLDVVLASSVQKDMASMIDEERALREAVRQMGVLKSEKMDLELLADDDRQCTKCKTTCFISAVFCSCSPGALVCLHHVEDLCDCPVYKYTLGYRYTVDDLYPMMNAVKLRAESYDSWATEVIDALKTSGNKQSLSCLKLLIQESEEKKFPENDLLRHLRVVVQDAEKCGTLAQQLLNGKRHTRFRSGGGKSANQLTVAELRSFVKQLHSLPCIITQTPQLKDLLCRVEEFQLQSQRLLTAEMPQACELQDLLDSSFQFDLDLPLVDIRERLEQARWLEEQHQACKDPRALTLDVMRRLIDLGVGLSPHPTVEKAMAKLQELLTMSEHWDDRCRSLLKSRPRQPLDSLTSALREVEKIPAFLPIVESLRDAVQRAHEWLQEVETVQAGPRVPVLDTLVELVAHGRAIPVHLAPLPRLEALVGEVHAWTECAANTFLTANSPYTLLEVLCPLCDVGTSGFKRKTKKLKEQVSLSGKKKYMKPDGLQELEKALSESRDTATAMAALGEARLREMEALHAFRISNENRLLSHEEQSDWKVCVCQGEPMAPMIQCELCRSCFHAGCITAPRHGAQVWLCPQCHRSEKPPLEKILPLLASLQRLRVRLPEGDALRYLIERTVSWQHRAQHMLSSNDLKMVQEKISSSQLYNRWQATAGHLTETNDVSQSVGATSFSLPHEWDNRSIYIRSPFSPGRNCIPLKSLNAELDELLMEAQLLQVSLPEVHELYHALLTEPSSTQAPECSLPNVTHSREKGPVRQQRADRSPVLTEAVNCIRKERSDSTDQKLKRRREGRMRKTPTFLKKRVKVAKPKDLCGRELLPEESRASDSQSSFTSDESDDDDAFCPADRCMEPEGDEVDWVQCDGNCNRWFHQVCVGLSAETAEKEDYICTHCKEMTCARK

Target ORF information:

RefSeq Version	XM_018091505.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine demethylase 5B (kdm5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091505.2

ORF Insert Sequence:

ATGGACAGGT CCGCTTCCCC TCTGTGCGTT TCATTATTCA GAATCCTACC TGCGGACCTC 
CAGTTGTTGT TAAAGTCCCA GCATCCCCTA ATTGTGTTGG GAATAGTAGG AGCTGGAGGC 
CCGCAGGTTG CCCTGTATTC ATGCTCCATC CATGTTAAGG ACTGGCAGCC ACCATTTGCT 
TGTGATGTGG ATAAACTGCA CTTTACTCCA AGGATCCAAA GACTAAATGA GCTTGAGGCT 
CAGACTAGAG TGAAGCTAAA TTTCCTAGAC CAGATTGCTA AATTTTGGGA GTTGCAGGGA 
TGTACTCTGA AAATTCCTCA CGTGGAGCGA AAGATTCTAG ATTTATTTCA GCTGAACAAG 
TTGGTGGCTG AAGATGGCGG ATTTGATTTA GTTTGTAGAG AAAGAAAATG GACCAAGATA 
GCTACAAAAA TGGGATTTTC ACTAGGAAAA GCCGTGGGTT CCCATATTCG AGGCCATTAT 
GAGCGAATTC TTCACCCTTA CAACTTGTTC CAATCTGGGA CCAACTTACT GGCTCCTGTG 
TCTTGCTCGA AGCATCCAGG GACCACAGAC AATAATTCTT ATGATAAGAA GCCATTTCTT 
GCACCTGATC TCAAGGACAA GGAGTACAAA CCACATGACA TCCCACAGCG ACAATCTGTC 
CAGCCCCCAG AAAACTGCAC TCCTGCCAGA AGGGCCAAAC GGATGAAATC TGAGGTTGTT 
ACCATTAAAA CTGAACCAGT GGAAGTTCCA GAACTAAGAC CACGTGCCCT TCGTCGTAGA 
ATGGGATACA CCCCAACTAA GTGTGAAAAG GAGGACAGTT TCCTTATAGT AAAGAAAGAG 
ATAGTGGAAC CAGTTATTGA GAAGCCCAAA GCACGGAATA AAAAATGTAG AAGCCAGGTG 
GATTTGTATG CTTGTCTTGT GTGTGGCAGT GGAAGTGATG AGGATCGACT TCTGCTGTGT 
GATGGTTGTG ATGATAGTTA CCACACTTTC TGCCTTATTC CTCCACTCCA AGATGTTCCC 
AAGGGAGACT GGAGATGCCC TAAATGCCTA GCACAGGAGT GTAGTAAACC ACAGGAAGCA 
TTTGGGTTTG AACAGGCATC CAGAGATTAC ACCCTACGCA TGTTTGGAGA GATGGCTGAT 
AACTTCAAAT CAGATTACTT TAACATGCCA GTGCATATGG TTCCCACAGA ACTGGTGGAA 
AAAGAGTTCT GGCGCTTGGT GAGCACCATA GAGGAGGATG TGACAGTAGA ATATGGGGCT 
GACATAGCTT CTAAGGAATT TGGAAGTGGC TTTCCTGTGC GTGATGGTAA ATTCCGTGTT 
AAACCAGAAG ATGAGGTATA TCTGAATTGC GGGTGGAATC TAAATAACAT GCCTATTATG 
CAGCCTTCTG TATTGGCACA TGTCACAGCA GATATATGTG GCATGAAATT ACCCTGGCTT 
TATGTGGGCA TGTGTTTCTC ATCCTTTTGC TGGCACATTG AGGACCACTG GAGCTACTCT 
ATTAATTATC TGCATTGGGG TGAGCCAAAA ACTTGGTATG GAGCACCTGG ATATGCTGCA 
GAACAGCTGG AGGAGGTCAT GAAAAAATTG GCGCCAGAAT TATTCATTGC ACAGCCTGAC 
CTCTTGCATC AGCTAGTGAC CATAATGAAT CCAAATACAC TAATGGCTCA TGGTGTTCCG 
ATTTATCGAA CCAATCAGTG TGCTGGAGAA TTTGTGATCA CTTTTCCACG TGCATATCAT 
AGTGGCTTTA ATCAAGGCTT TAACTTTGCA GAAGCCGTCA ACTTCTGCAC TGTTGATTGG 
CTTCCTCTTG GCCGTCAGTG CATTGAACAT TACAGAGGGC TAAACCGATA CTGTGTATTT 
TCCCATGATG AGATGATCTG TAAAATGGCC ATTAAGGCAG ATAAACTTGA TGTTGTACTA 
GCATCTTCTG TACAGAAAGA TATGGCTAGC ATGATAGACG AGGAGAGAGC CCTGCGTGAA 
GCTGTCCGAC AGATGGGGGT TTTGAAGTCA GAAAAGATGG ACCTTGAACT CCTAGCAGAT 
GATGATCGGC AGTGTACAAA GTGTAAGACC ACCTGTTTCA TATCAGCAGT GTTCTGTTCG 
TGTAGCCCTG GGGCCCTTGT ATGTCTTCAT CATGTGGAGG ATCTCTGTGA TTGTCCAGTC 
TACAAGTACA CATTGGGGTA TCGTTACACT GTTGATGATT TATATCCCAT GATGAACGCT 
GTTAAGCTGC GAGCAGAGTC ATATGACAGT TGGGCAACAG AAGTCATTGA TGCTCTCAAA 
ACTTCAGGCA ATAAACAAAG TCTCTCCTGC CTGAAGTTAT TAATTCAGGA ATCCGAGGAA 
AAGAAATTTC CAGAAAATGA TCTTTTACGC CACCTACGGG TTGTTGTACA GGATGCTGAG 
AAATGTGGAA CATTGGCGCA ACAGCTACTT AATGGGAAGC GTCATACCAG GTTCAGGTCA 
GGTGGTGGGA AGTCGGCAAA CCAGCTAACT GTTGCCGAAC TCAGGTCTTT TGTAAAGCAG 
CTTCACTCTC TGCCCTGTAT TATCACCCAG ACACCTCAGC TTAAGGATCT GCTTTGTCGT 
GTGGAAGAAT TCCAGTTACA AAGCCAAAGA CTTCTAACAG CAGAAATGCC ACAAGCTTGT 
GAACTTCAAG ATCTGCTTGA CTCTAGTTTT CAGTTTGACT TGGACTTGCC ACTGGTAGAT 
ATTCGGGAAC GCCTTGAACA GGCTAGATGG CTAGAGGAGC AGCATCAGGC ATGCAAGGAT 
CCTAGGGCAC TTACATTGGA TGTCATGAGG AGACTTATAG ATTTGGGTGT GGGTCTCTCT 
CCTCACCCTA CTGTAGAAAA GGCAATGGCC AAACTTCAGG AGCTGCTTAC TATGTCTGAA 
CATTGGGATG ATCGGTGTAG AAGCCTTCTC AAATCCAGGC CCCGGCAACC ACTTGATTCC 
TTAACATCAG CTTTAAGAGA AGTGGAAAAA ATCCCAGCTT TTTTGCCTAT TGTGGAATCC 
TTAAGAGATG CTGTACAAAG GGCTCATGAA TGGTTGCAGG AAGTGGAGAC TGTACAGGCT 
GGGCCCAGGG TTCCTGTGTT GGACACATTG GTGGAACTTG TCGCCCATGG ACGGGCAATT 
CCTGTTCATC TGGCACCTCT GCCAAGGCTA GAGGCATTAG TGGGAGAAGT CCATGCTTGG 
ACTGAATGTG CAGCCAACAC TTTTTTAACA GCCAACTCCC CATACACACT GCTTGAGGTC 
CTTTGCCCTC TTTGTGATGT TGGTACCTCT GGCTTTAAAA GGAAAACAAA GAAACTTAAA 
GAGCAGGTGT CCTTAAGTGG AAAGAAAAAG TATATGAAGC CAGATGGTCT GCAGGAACTG 
GAAAAAGCAT TAAGTGAAAG CAGAGACACT GCCACAGCGA TGGCAGCCTT AGGAGAAGCC 
CGACTACGTG AGATGGAGGC CTTGCATGCT TTCAGGATTT CTAATGAGAA CAGACTTCTA 
TCCCATGAGG AACAGAGTGA CTGGAAGGTG TGCGTCTGCC AGGGAGAACC AATGGCTCCT 
ATGATCCAGT GTGAGCTGTG CAGAAGCTGC TTTCACGCTG GTTGTATTAC AGCTCCTCGA 
CATGGAGCTC AGGTCTGGCT ATGTCCTCAG TGCCACCGCT CTGAAAAACC TCCTTTGGAG 
AAGATCCTGC CTCTGTTAGC TTCCCTGCAA CGTCTGCGTG TCCGTCTGCC AGAAGGAGAT 
GCCTTACGTT ACTTGATTGA AAGGACAGTC AGTTGGCAAC ATCGCGCACA ACATATGTTG 
TCTTCAAACG ACCTGAAAAT GGTGCAAGAG AAAATTAGCT CGAGCCAGTT ATACAACAGG 
TGGCAAGCAA CAGCCGGGCA TTTGACAGAG ACCAATGATG TATCGCAGAG TGTAGGGGCC 
ACATCATTCT CTCTACCTCA TGAGTGGGAC AACAGAAGCA TTTATATCCG ATCCCCATTT 
TCTCCTGGAC GCAACTGCAT CCCACTGAAA AGTCTGAATG CTGAATTAGA TGAATTGTTA 
ATGGAAGCTC AGCTTTTGCA AGTTTCACTT CCTGAGGTCC ATGAGCTCTA TCATGCACTT 
CTGACAGAGC CTTCTTCCAC ACAAGCCCCA GAATGCAGCT TACCCAATGT TACCCATAGC 
AGGGAAAAAG GACCTGTGAG ACAGCAGAGA GCAGATCGAA GCCCAGTATT AACAGAGGCT 
GTAAACTGCA TACGGAAAGA AAGATCAGAT AGTACAGACC AAAAGTTGAA GCGCCGTCGT 
GAAGGAAGAA TGAGAAAGAC TCCTACTTTC CTCAAAAAGA GGGTTAAAGT AGCTAAACCA 
AAAGACTTAT GTGGCAGAGA GCTACTTCCT GAGGAAAGCA GAGCAAGTGA TTCCCAATCC 
TCCTTTACAT CAGATGAGTC TGATGATGAC GATGCATTTT GCCCTGCAGA CAGATGTATG 
GAACCTGAAG GTGATGAGGT TGACTGGGTA CAGTGTGATG GGAATTGCAA TCGTTGGTTT 
CATCAGGTTT GTGTTGGTCT TTCTGCGGAA ACAGCTGAAA AGGAGGATTA CATTTGTACA 
CACTGTAAGG AGATGACTTG TGCACGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5b ( XM_018091505.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946994.1 Xenopus tropicalis lysine (K)-specific demethylase 5B (kdm5b), transcript variant X1, mRNA.

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CloneID	OXa42705
Clone ID Related Accession (Same CDS sequence)	XM_018091505.2 , XM_018091505.1
Accession Version	XM_018091505.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGT CCGCTTCCCC TCTGTGCGTT TCATTATTCA GAATCCTACC TGCGGACCTC 
CAGTTGTTGT TAAAGTCCCA GCATCCCCTA ATTGTGTTGG GAATAGTAGG AGCTGGAGGC 
CCGCAGGTTG CCCTGTATTC ATGCTCCATC CATGTTAAGG ACTGGCAGCC ACCATTTGCT 
TGTGATGTGG ATAAACTGCA CTTTACTCCA AGGATCCAAA GACTAAATGA GCTTGAGGCT 
CAGACTAGAG TGAAGCTAAA TTTCCTAGAC CAGATTGCTA AATTTTGGGA GTTGCAGGGA 
TGTACTCTGA AAATTCCTCA CGTGGAGCGA AAGATTCTAG ATTTATTTCA GCTGAACAAG 
TTGGTGGCTG AAGATGGCGG ATTTGATTTA GTTTGTAGAG AAAGAAAATG GACCAAGATA 
GCTACAAAAA TGGGATTTTC ACTAGGAAAA GCCGTGGGTT CCCATATTCG AGGCCATTAT 
GAGCGAATTC TTCACCCTTA CAACTTGTTC CAATCTGGGA CCAACTTACT GGCTCCTGTG 
TCTTGCTCGA AGCATCCAGG GACCACAGAC AATAATTCTT ATGATAAGAA GCCATTTCTT 
GCACCTGATC TCAAGGACAA GGAGTACAAA CCACATGACA TCCCACAGCG ACAATCTGTC 
CAGCCCCCAG AAAACTGCAC TCCTGCCAGA AGGGCCAAAC GGATGAAATC TGAGGTTGTT 
ACCATTAAAA CTGAACCAGT GGAAGTTCCA GAACTAAGAC CACGTGCCCT TCGTCGTAGA 
ATGGGATACA CCCCAACTAA GTGTGAAAAG GAGGACAGTT TCCTTATAGT AAAGAAAGAG 
ATAGTGGAAC CAGTTATTGA GAAGCCCAAA GCACGGAATA AAAAATGTAG AAGCCAGGTG 
GATTTGTATG CTTGTCTTGT GTGTGGCAGT GGAAGTGATG AGGATCGACT TCTGCTGTGT 
GATGGTTGTG ATGATAGTTA CCACACTTTC TGCCTTATTC CTCCACTCCA AGATGTTCCC 
AAGGGAGACT GGAGATGCCC TAAATGCCTA GCACAGGAGT GTAGTAAACC ACAGGAAGCA 
TTTGGGTTTG AACAGGCATC CAGAGATTAC ACCCTACGCA TGTTTGGAGA GATGGCTGAT 
AACTTCAAAT CAGATTACTT TAACATGCCA GTGCATATGG TTCCCACAGA ACTGGTGGAA 
AAAGAGTTCT GGCGCTTGGT GAGCACCATA GAGGAGGATG TGACAGTAGA ATATGGGGCT 
GACATAGCTT CTAAGGAATT TGGAAGTGGC TTTCCTGTGC GTGATGGTAA ATTCCGTGTT 
AAACCAGAAG ATGAGGTATA TCTGAATTGC GGGTGGAATC TAAATAACAT GCCTATTATG 
CAGCCTTCTG TATTGGCACA TGTCACAGCA GATATATGTG GCATGAAATT ACCCTGGCTT 
TATGTGGGCA TGTGTTTCTC ATCCTTTTGC TGGCACATTG AGGACCACTG GAGCTACTCT 
ATTAATTATC TGCATTGGGG TGAGCCAAAA ACTTGGTATG GAGCACCTGG ATATGCTGCA 
GAACAGCTGG AGGAGGTCAT GAAAAAATTG GCGCCAGAAT TATTCATTGC ACAGCCTGAC 
CTCTTGCATC AGCTAGTGAC CATAATGAAT CCAAATACAC TAATGGCTCA TGGTGTTCCG 
ATTTATCGAA CCAATCAGTG TGCTGGAGAA TTTGTGATCA CTTTTCCACG TGCATATCAT 
AGTGGCTTTA ATCAAGGCTT TAACTTTGCA GAAGCCGTCA ACTTCTGCAC TGTTGATTGG 
CTTCCTCTTG GCCGTCAGTG CATTGAACAT TACAGAGGGC TAAACCGATA CTGTGTATTT 
TCCCATGATG AGATGATCTG TAAAATGGCC ATTAAGGCAG ATAAACTTGA TGTTGTACTA 
GCATCTTCTG TACAGAAAGA TATGGCTAGC ATGATAGACG AGGAGAGAGC CCTGCGTGAA 
GCTGTCCGAC AGATGGGGGT TTTGAAGTCA GAAAAGATGG ACCTTGAACT CCTAGCAGAT 
GATGATCGGC AGTGTACAAA GTGTAAGACC ACCTGTTTCA TATCAGCAGT GTTCTGTTCG 
TGTAGCCCTG GGGCCCTTGT ATGTCTTCAT CATGTGGAGG ATCTCTGTGA TTGTCCAGTC 
TACAAGTACA CATTGGGGTA TCGTTACACT GTTGATGATT TATATCCCAT GATGAACGCT 
GTTAAGCTGC GAGCAGAGTC ATATGACAGT TGGGCAACAG AAGTCATTGA TGCTCTCAAA 
ACTTCAGGCA ATAAACAAAG TCTCTCCTGC CTGAAGTTAT TAATTCAGGA ATCCGAGGAA 
AAGAAATTTC CAGAAAATGA TCTTTTACGC CACCTACGGG TTGTTGTACA GGATGCTGAG 
AAATGTGGAA CATTGGCGCA ACAGCTACTT AATGGGAAGC GTCATACCAG GTTCAGGTCA 
GGTGGTGGGA AGTCGGCAAA CCAGCTAACT GTTGCCGAAC TCAGGTCTTT TGTAAAGCAG 
CTTCACTCTC TGCCCTGTAT TATCACCCAG ACACCTCAGC TTAAGGATCT GCTTTGTCGT 
GTGGAAGAAT TCCAGTTACA AAGCCAAAGA CTTCTAACAG CAGAAATGCC ACAAGCTTGT 
GAACTTCAAG ATCTGCTTGA CTCTAGTTTT CAGTTTGACT TGGACTTGCC ACTGGTAGAT 
ATTCGGGAAC GCCTTGAACA GGCTAGATGG CTAGAGGAGC AGCATCAGGC ATGCAAGGAT 
CCTAGGGCAC TTACATTGGA TGTCATGAGG AGACTTATAG ATTTGGGTGT GGGTCTCTCT 
CCTCACCCTA CTGTAGAAAA GGCAATGGCC AAACTTCAGG AGCTGCTTAC TATGTCTGAA 
CATTGGGATG ATCGGTGTAG AAGCCTTCTC AAATCCAGGC CCCGGCAACC ACTTGATTCC 
TTAACATCAG CTTTAAGAGA AGTGGAAAAA ATCCCAGCTT TTTTGCCTAT TGTGGAATCC 
TTAAGAGATG CTGTACAAAG GGCTCATGAA TGGTTGCAGG AAGTGGAGAC TGTACAGGCT 
GGGCCCAGGG TTCCTGTGTT GGACACATTG GTGGAACTTG TCGCCCATGG ACGGGCAATT 
CCTGTTCATC TGGCACCTCT GCCAAGGCTA GAGGCATTAG TGGGAGAAGT CCATGCTTGG 
ACTGAATGTG CAGCCAACAC TTTTTTAACA GCCAACTCCC CATACACACT GCTTGAGGTC 
CTTTGCCCTC TTTGTGATGT TGGTACCTCT GGCTTTAAAA GGAAAACAAA GAAACTTAAA 
GAGCAGGTGT CCTTAAGTGG AAAGAAAAAG TATATGAAGC CAGATGGTCT GCAGGAACTG 
GAAAAAGCAT TAAGTGAAAG CAGAGACACT GCCACAGCGA TGGCAGCCTT AGGAGAAGCC 
CGACTACGTG AGATGGAGGC CTTGCATGCT TTCAGGATTT CTAATGAGAA CAGACTTCTA 
TCCCATGAGG AACAGAGTGA CTGGAAGGTG TGCGTCTGCC AGGGAGAACC AATGGCTCCT 
ATGATCCAGT GTGAGCTGTG CAGAAGCTGC TTTCACGCTG GTTGTATTAC AGCTCCTCGA 
CATGGAGCTC AGGTCTGGCT ATGTCCTCAG TGCCACCGCT CTGAAAAACC TCCTTTGGAG 
AAGATCCTGC CTCTGTTAGC TTCCCTGCAA CGTCTGCGTG TCCGTCTGCC AGAAGGAGAT 
GCCTTACGTT ACTTGATTGA AAGGACAGTC AGTTGGCAAC ATCGCGCACA ACATATGTTG 
TCTTCAAACG ACCTGAAAAT GGTGCAAGAG AAAATTAGCT CGAGCCAGTT ATACAACAGG 
TGGCAAGCAA CAGCCGGGCA TTTGACAGAG ACCAATGATG TATCGCAGAG TGTAGGGGCC 
ACATCATTCT CTCTACCTCA TGAGTGGGAC AACAGAAGCA TTTATATCCG ATCCCCATTT 
TCTCCTGGAC GCAACTGCAT CCCACTGAAA AGTCTGAATG CTGAATTAGA TGAATTGTTA 
ATGGAAGCTC AGCTTTTGCA AGTTTCACTT CCTGAGGTCC ATGAGCTCTA TCATGCACTT 
CTGACAGAGC CTTCTTCCAC ACAAGCCCCA GAATGCAGCT TACCCAATGT TACCCATAGC 
AGGGAAAAAG GACCTGTGAG ACAGCAGAGA GCAGATCGAA GCCCAGTATT AACAGAGGCT 
GTAAACTGCA TACGGAAAGA AAGATCAGAT AGTACAGACC AAAAGTTGAA GCGCCGTCGT 
GAAGGAAGAA TGAGAAAGAC TCCTACTTTC CTCAAAAAGA GGGTTAAAGT AGCTAAACCA 
AAAGACTTAT GTGGCAGAGA GCTACTTCCT GAGGAAAGCA GAGCAAGTGA TTCCCAATCC 
TCCTTTACAT CAGATGAGTC TGATGATGAC GATGCATTTT GCCCTGCAGA CAGATGTATG 
GAACCTGAAG GTGATGAGGT TGACTGGGTA CAGTGTGATG GGAATTGCAA TCGTTGGTTT 
CATCAGGTTT GTGTTGGTCT TTCTGCGGAA ACAGCTGAAA AGGAGGATTA CATTTGTACA 
CACTGTAAGG AGATGACTTG TGCACGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946994.1
CDS	18..4610
Translation	MDRSASPLCVSLFRILPADLQLLLKSQHPLIVLGIVGAGGPQVALYSCSIHVKDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWELQGCTLKIPHVERKILDLFQLNKLVAEDGGFDLVCRERKWTKIATKMGFSLGKAVGSHIRGHYERILHPYNLFQSGTNLLAPVSCSKHPGTTDNNSYDKKPFLAPDLKDKEYKPHDIPQRQSVQPPENCTPARRAKRMKSEVVTIKTEPVEVPELRPRALRRRMGYTPTKCEKEDSFLIVKKEIVEPVIEKPKARNKKCRSQVDLYACLVCGSGSDEDRLLLCDGCDDSYHTFCLIPPLQDVPKGDWRCPKCLAQECSKPQEAFGFEQASRDYTLRMFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKFRVKPEDEVYLNCGWNLNNMPIMQPSVLAHVTADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGYAAEQLEEVMKKLAPELFIAQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCIEHYRGLNRYCVFSHDEMICKMAIKADKLDVVLASSVQKDMASMIDEERALREAVRQMGVLKSEKMDLELLADDDRQCTKCKTTCFISAVFCSCSPGALVCLHHVEDLCDCPVYKYTLGYRYTVDDLYPMMNAVKLRAESYDSWATEVIDALKTSGNKQSLSCLKLLIQESEEKKFPENDLLRHLRVVVQDAEKCGTLAQQLLNGKRHTRFRSGGGKSANQLTVAELRSFVKQLHSLPCIITQTPQLKDLLCRVEEFQLQSQRLLTAEMPQACELQDLLDSSFQFDLDLPLVDIRERLEQARWLEEQHQACKDPRALTLDVMRRLIDLGVGLSPHPTVEKAMAKLQELLTMSEHWDDRCRSLLKSRPRQPLDSLTSALREVEKIPAFLPIVESLRDAVQRAHEWLQEVETVQAGPRVPVLDTLVELVAHGRAIPVHLAPLPRLEALVGEVHAWTECAANTFLTANSPYTLLEVLCPLCDVGTSGFKRKTKKLKEQVSLSGKKKYMKPDGLQELEKALSESRDTATAMAALGEARLREMEALHAFRISNENRLLSHEEQSDWKVCVCQGEPMAPMIQCELCRSCFHAGCITAPRHGAQVWLCPQCHRSEKPPLEKILPLLASLQRLRVRLPEGDALRYLIERTVSWQHRAQHMLSSNDLKMVQEKISSSQLYNRWQATAGHLTETNDVSQSVGATSFSLPHEWDNRSIYIRSPFSPGRNCIPLKSLNAELDELLMEAQLLQVSLPEVHELYHALLTEPSSTQAPECSLPNVTHSREKGPVRQQRADRSPVLTEAVNCIRKERSDSTDQKLKRRREGRMRKTPTFLKKRVKVAKPKDLCGRELLPEESRASDSQSSFTSDESDDDDAFCPADRCMEPEGDEVDWVQCDGNCNRWFHQVCVGLSAETAEKEDYICTHCKEMTCARK

Target ORF information:

RefSeq Version	XM_018091505.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 5B (kdm5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091505.1

ORF Insert Sequence:

ATGGACAGGT CCGCTTCCCC TCTGTGCGTT TCATTATTCA GAATCCTACC TGCGGACCTC 
CAGTTGTTGT TAAAGTCCCA GCATCCCCTA ATTGTGTTGG GAATAGTAGG AGCTGGAGGC 
CCGCAGGTTG CCCTGTATTC ATGCTCCATC CATGTTAAGG ACTGGCAGCC ACCATTTGCT 
TGTGATGTGG ATAAACTGCA CTTTACTCCA AGGATCCAAA GACTAAATGA GCTTGAGGCT 
CAGACTAGAG TGAAGCTAAA TTTCCTAGAC CAGATTGCTA AATTTTGGGA GTTGCAGGGA 
TGTACTCTGA AAATTCCTCA CGTGGAGCGA AAGATTCTAG ATTTATTTCA GCTGAACAAG 
TTGGTGGCTG AAGATGGCGG ATTTGATTTA GTTTGTAGAG AAAGAAAATG GACCAAGATA 
GCTACAAAAA TGGGATTTTC ACTAGGAAAA GCCGTGGGTT CCCATATTCG AGGCCATTAT 
GAGCGAATTC TTCACCCTTA CAACTTGTTC CAATCTGGGA CCAACTTACT GGCTCCTGTG 
TCTTGCTCGA AGCATCCAGG GACCACAGAC AATAATTCTT ATGATAAGAA GCCATTTCTT 
GCACCTGATC TCAAGGACAA GGAGTACAAA CCACATGACA TCCCACAGCG ACAATCTGTC 
CAGCCCCCAG AAAACTGCAC TCCTGCCAGA AGGGCCAAAC GGATGAAATC TGAGGTTGTT 
ACCATTAAAA CTGAACCAGT GGAAGTTCCA GAACTAAGAC CACGTGCCCT TCGTCGTAGA 
ATGGGATACA CCCCAACTAA GTGTGAAAAG GAGGACAGTT TCCTTATAGT AAAGAAAGAG 
ATAGTGGAAC CAGTTATTGA GAAGCCCAAA GCACGGAATA AAAAATGTAG AAGCCAGGTG 
GATTTGTATG CTTGTCTTGT GTGTGGCAGT GGAAGTGATG AGGATCGACT TCTGCTGTGT 
GATGGTTGTG ATGATAGTTA CCACACTTTC TGCCTTATTC CTCCACTCCA AGATGTTCCC 
AAGGGAGACT GGAGATGCCC TAAATGCCTA GCACAGGAGT GTAGTAAACC ACAGGAAGCA 
TTTGGGTTTG AACAGGCATC CAGAGATTAC ACCCTACGCA TGTTTGGAGA GATGGCTGAT 
AACTTCAAAT CAGATTACTT TAACATGCCA GTGCATATGG TTCCCACAGA ACTGGTGGAA 
AAAGAGTTCT GGCGCTTGGT GAGCACCATA GAGGAGGATG TGACAGTAGA ATATGGGGCT 
GACATAGCTT CTAAGGAATT TGGAAGTGGC TTTCCTGTGC GTGATGGTAA ATTCCGTGTT 
AAACCAGAAG ATGAGGTATA TCTGAATTGC GGGTGGAATC TAAATAACAT GCCTATTATG 
CAGCCTTCTG TATTGGCACA TGTCACAGCA GATATATGTG GCATGAAATT ACCCTGGCTT 
TATGTGGGCA TGTGTTTCTC ATCCTTTTGC TGGCACATTG AGGACCACTG GAGCTACTCT 
ATTAATTATC TGCATTGGGG TGAGCCAAAA ACTTGGTATG GAGCACCTGG ATATGCTGCA 
GAACAGCTGG AGGAGGTCAT GAAAAAATTG GCGCCAGAAT TATTCATTGC ACAGCCTGAC 
CTCTTGCATC AGCTAGTGAC CATAATGAAT CCAAATACAC TAATGGCTCA TGGTGTTCCG 
ATTTATCGAA CCAATCAGTG TGCTGGAGAA TTTGTGATCA CTTTTCCACG TGCATATCAT 
AGTGGCTTTA ATCAAGGCTT TAACTTTGCA GAAGCCGTCA ACTTCTGCAC TGTTGATTGG 
CTTCCTCTTG GCCGTCAGTG CATTGAACAT TACAGAGGGC TAAACCGATA CTGTGTATTT 
TCCCATGATG AGATGATCTG TAAAATGGCC ATTAAGGCAG ATAAACTTGA TGTTGTACTA 
GCATCTTCTG TACAGAAAGA TATGGCTAGC ATGATAGACG AGGAGAGAGC CCTGCGTGAA 
GCTGTCCGAC AGATGGGGGT TTTGAAGTCA GAAAAGATGG ACCTTGAACT CCTAGCAGAT 
GATGATCGGC AGTGTACAAA GTGTAAGACC ACCTGTTTCA TATCAGCAGT GTTCTGTTCG 
TGTAGCCCTG GGGCCCTTGT ATGTCTTCAT CATGTGGAGG ATCTCTGTGA TTGTCCAGTC 
TACAAGTACA CATTGGGGTA TCGTTACACT GTTGATGATT TATATCCCAT GATGAACGCT 
GTTAAGCTGC GAGCAGAGTC ATATGACAGT TGGGCAACAG AAGTCATTGA TGCTCTCAAA 
ACTTCAGGCA ATAAACAAAG TCTCTCCTGC CTGAAGTTAT TAATTCAGGA ATCCGAGGAA 
AAGAAATTTC CAGAAAATGA TCTTTTACGC CACCTACGGG TTGTTGTACA GGATGCTGAG 
AAATGTGGAA CATTGGCGCA ACAGCTACTT AATGGGAAGC GTCATACCAG GTTCAGGTCA 
GGTGGTGGGA AGTCGGCAAA CCAGCTAACT GTTGCCGAAC TCAGGTCTTT TGTAAAGCAG 
CTTCACTCTC TGCCCTGTAT TATCACCCAG ACACCTCAGC TTAAGGATCT GCTTTGTCGT 
GTGGAAGAAT TCCAGTTACA AAGCCAAAGA CTTCTAACAG CAGAAATGCC ACAAGCTTGT 
GAACTTCAAG ATCTGCTTGA CTCTAGTTTT CAGTTTGACT TGGACTTGCC ACTGGTAGAT 
ATTCGGGAAC GCCTTGAACA GGCTAGATGG CTAGAGGAGC AGCATCAGGC ATGCAAGGAT 
CCTAGGGCAC TTACATTGGA TGTCATGAGG AGACTTATAG ATTTGGGTGT GGGTCTCTCT 
CCTCACCCTA CTGTAGAAAA GGCAATGGCC AAACTTCAGG AGCTGCTTAC TATGTCTGAA 
CATTGGGATG ATCGGTGTAG AAGCCTTCTC AAATCCAGGC CCCGGCAACC ACTTGATTCC 
TTAACATCAG CTTTAAGAGA AGTGGAAAAA ATCCCAGCTT TTTTGCCTAT TGTGGAATCC 
TTAAGAGATG CTGTACAAAG GGCTCATGAA TGGTTGCAGG AAGTGGAGAC TGTACAGGCT 
GGGCCCAGGG TTCCTGTGTT GGACACATTG GTGGAACTTG TCGCCCATGG ACGGGCAATT 
CCTGTTCATC TGGCACCTCT GCCAAGGCTA GAGGCATTAG TGGGAGAAGT CCATGCTTGG 
ACTGAATGTG CAGCCAACAC TTTTTTAACA GCCAACTCCC CATACACACT GCTTGAGGTC 
CTTTGCCCTC TTTGTGATGT TGGTACCTCT GGCTTTAAAA GGAAAACAAA GAAACTTAAA 
GAGCAGGTGT CCTTAAGTGG AAAGAAAAAG TATATGAAGC CAGATGGTCT GCAGGAACTG 
GAAAAAGCAT TAAGTGAAAG CAGAGACACT GCCACAGCGA TGGCAGCCTT AGGAGAAGCC 
CGACTACGTG AGATGGAGGC CTTGCATGCT TTCAGGATTT CTAATGAGAA CAGACTTCTA 
TCCCATGAGG AACAGAGTGA CTGGAAGGTG TGCGTCTGCC AGGGAGAACC AATGGCTCCT 
ATGATCCAGT GTGAGCTGTG CAGAAGCTGC TTTCACGCTG GTTGTATTAC AGCTCCTCGA 
CATGGAGCTC AGGTCTGGCT ATGTCCTCAG TGCCACCGCT CTGAAAAACC TCCTTTGGAG 
AAGATCCTGC CTCTGTTAGC TTCCCTGCAA CGTCTGCGTG TCCGTCTGCC AGAAGGAGAT 
GCCTTACGTT ACTTGATTGA AAGGACAGTC AGTTGGCAAC ATCGCGCACA ACATATGTTG 
TCTTCAAACG ACCTGAAAAT GGTGCAAGAG AAAATTAGCT CGAGCCAGTT ATACAACAGG 
TGGCAAGCAA CAGCCGGGCA TTTGACAGAG ACCAATGATG TATCGCAGAG TGTAGGGGCC 
ACATCATTCT CTCTACCTCA TGAGTGGGAC AACAGAAGCA TTTATATCCG ATCCCCATTT 
TCTCCTGGAC GCAACTGCAT CCCACTGAAA AGTCTGAATG CTGAATTAGA TGAATTGTTA 
ATGGAAGCTC AGCTTTTGCA AGTTTCACTT CCTGAGGTCC ATGAGCTCTA TCATGCACTT 
CTGACAGAGC CTTCTTCCAC ACAAGCCCCA GAATGCAGCT TACCCAATGT TACCCATAGC 
AGGGAAAAAG GACCTGTGAG ACAGCAGAGA GCAGATCGAA GCCCAGTATT AACAGAGGCT 
GTAAACTGCA TACGGAAAGA AAGATCAGAT AGTACAGACC AAAAGTTGAA GCGCCGTCGT 
GAAGGAAGAA TGAGAAAGAC TCCTACTTTC CTCAAAAAGA GGGTTAAAGT AGCTAAACCA 
AAAGACTTAT GTGGCAGAGA GCTACTTCCT GAGGAAAGCA GAGCAAGTGA TTCCCAATCC 
TCCTTTACAT CAGATGAGTC TGATGATGAC GATGCATTTT GCCCTGCAGA CAGATGTATG 
GAACCTGAAG GTGATGAGGT TGACTGGGTA CAGTGTGATG GGAATTGCAA TCGTTGGTTT 
CATCAGGTTT GTGTTGGTCT TTCTGCGGAA ACAGCTGAAA AGGAGGATTA CATTTGTACA 
CACTGTAAGG AGATGACTTG TGCACGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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kdm5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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