tmc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmc1
Entrez Gene ID	100486683
Full Name	transmembrane channel like 1
Synonyms	dfna36,dfnb11,dfnb7
General protein information	Preferred Names transmembrane channel like 1 Names transmembrane channel-like protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935593.1	XP_002935639.1	transmembrane channel-like protein 1
XM_002935593.2	XP_002935639.1	transmembrane channel-like protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tmc1	NP_083229.1
Canis lupus familiaris (dog)	TMC1	XP_005616877.1
Bos taurus (cattle)	TMC1	XP_002689695.2
Danio rerio (zebrafish)	tmc1	XP_695621.4
Rattus norvegicus (Norway rat)	Tmc1	NP_001101991.1
Xenopus tropicalis (tropical clawed frog)	tmc1	XP_002935639.1
Homo sapiens (human)	TMC1	NP_619636.2
Pan troglodytes (chimpanzee)	TMC1	XP_528322.2
Macaca mulatta (Rhesus monkey)	TMC1	XP_001093188.2
Gallus gallus (chicken)	TMC1	NP_001006580.1

Gene Ontology

The following tmc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa05107	XM_002935593.2 Latest version!	Xenopus tropicalis transmembrane channel like 1 (tmc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa05107	XM_002935593.1	Xenopus tropicalis transmembrane channel like 1 (tmc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmc1 ( XM_002935593.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935639.1 Xenopus tropicalis transmembrane channel like 1 (tmc1), mRNA.

Price & Availability↑

CloneID	OXa05107
Clone ID Related Accession (Same CDS sequence)	XM_002935593.1 , XM_002935593.2
Accession Version	XM_002935593.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transmembrane channel-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 22% of CDS bases ##RefSeq-Attributes-END##

ATGGGCTGTT ATCTGATGGC CATTCAGAAC ATCGTCTCTG ACTGCAAGAC CTTGAATGGT 
CTCACACCAG CTATCGGAAG CAGAGCTGAG ATGCCTAAAC ACAAAAAGCT TGTGGCCTCT 
GAAAATGACG TTCAAATTGA CGTGGACAAT GAAGAGGACA ATGCAGATTG TGAGGAGGAA 
GAAGAAGAAC AGCCTCCAAA GCGAAGGGAG AAAAACTGGA GAGTACCAGA CATGAAACCA 
TGGCTGGCAG AAATGACAGA TGATGAAAAC AGAAATGCCA ATGCAGAGGA GCCCAATGAG 
AATGGGGAAG GCAGCCCTGG GGGAAGGACT GGCAGAAAAG GAAGGAAGGG ACCTGGAGCA 
GCCAAGAGAA AGCCAAGGGA TAATTCCAAG AAGAAGAAAA AGAAAGATGC AGATGGATCT 
GACTCTGATG AAGAAGCCAT CACAGAAGAG GAATTAGACA AACTAAAGGA AGCCGTAGAA 
GAAAAAAAGA AACTCATTGC CACTATAAGA AGCAAACCTT GGAAAATGAA GCAAAAACTA 
GAAGTCCTCA AGGATGCGCA AGAGTTTGTG GAAAAATTTG AAGGAGCTTT GGGGAAAGGA 
AAAGGGAAGA AGTTTTACGC ATACAAGGTT ATGATGATGA AAAAATGGAT GAAGTTTCAA 
AGGGATTTTG AAAATTTCAG AACTGCCTGT ATACCCTGGG AGATGAAAAT CAAGGAAATT 
GAAAGTCATT TTGGTTCTTC AGTTGCTTCC TATTTTATAT TCCTACGCTG GATGTATGGG 
ATTAATTTGA TACTGTTTGG ACTAACCTTT GGCCTCGTCA TGATACCAGA GGCATTAATG 
GGAAAGCCAT ATGGTTCAAT GCCAAGAAAA ACTGTTCCAC GGCCTGAGCA GCCGACTGCA 
ATGAATTTTG CCACGCTTTG GGATTTCAGT GGATTTGCAC AATATTCTGT TCTTTTCTAT 
GGATACTACA ACAGTCAGAG AACAATTGGA TGGCTACAGT TTAGGCTGCC ACTTTCTTAC 
TTACTTGTTG GAATTGGTAC CATTGCATAC AGTTTCATGG TTGTTATCCG AACCATGGCC 
AAAAATGCTA ATGATGATGG AGGTGGTGAT GACAACAGCT TTAATTTTAG CTGGAAAATG 
TACACGAGTT GGGACTATCT TATTGGCAAC CCAGAAACAG CTGACAATAA ATTTGCATCA 
ATTACCACAA GCTTCAAGGA ATCGATTTTG GAAGAACAGG AAAACAGGAA AGAGGAAAAT 
ATTCATTTAG TTAGATTCCT CAGAGTCTTT GCAAACTTTC TTGTGATGTG CACTCTTGCA 
GGCAGTGGAT ACTTAATTTA CTTTGTTGTA AGGAGATCAC AACAATTTGC ATTGGAAGGT 
CTAGAAAACT ATGGATGGTG GGAACGGAAT GAAGTGAACA CAGTCATGTC ACTGCTAGGA 
ATGTTCTGCC CAACAATGTT TGATGTAATT AGTAATTTGG AGAATTATCA CCCAAGAATA 
GCACTGAGGT GGCAACTAGG ACGCATATTT GCTTTATTTT TGGGTAACCT GTATACATTT 
CTTATTGCCC TTATGGATGA AATCAATCTT AAGAGGGAAG AAGAGAAGAT TGTTCAACAT 
AATATGACAT TTTGGGAGGC AAATATTTAT AACAAATCCT ACTCTGAAAA TTCAACAGTC 
CCTTTCCAGG TAGATCCATC AGATGTTCCT CGAGGACCAT GTTGGGAAAC CATGGTGGGA 
CAGGAATTTG TTCGGCTTAC AGTTTCTGAT ATTATGACAT CTTATATTAC TATTCTAACT 
GGCGACTTCC TACAAGCTGT ATTTGTACGC TTTTTTAATT ACTGCTGGTG CTGGGATCTA 
GAATATGGAT GGCCATCGTA TTGCGAGTTC GACATCAGTG GCAATGTGCT GGGCCTCATT 
TTCAATCAAG GGATGATCTG GATGGGGTCA TTTTACGCTC CATGCCTGCC TGCTATTAAT 
GTATTTCGAC TTCACACATC CATGTACTTG CAGTGCTGGG CAGTAATGTG CTGCAATGTT 
CCCCATGCAC GAGTGTTTAA GGCATCCCGC TCCAATAACT TCTATATGGC TATGCTTCTT 
TTTGTTCTAT TTCTGGCCAT GTTACCAGTC GTCTATACCA TTGTCTCCAT TCCACCATCC 
TTTGACTGTG GCCCATTCAG TGGAAAACAA AGAATGTTTG AAGTCATACA AGAGACTCTG 
GCTAATGACT TCCCAGCCTG GTTTGCAAAA GTATTTAGCT ATGCATCAAA TCCCGGCCTT 
ATTCTACCTT TCATCCTCCT TATGGTAATG AGTATCTATT ACCTGAATGC TACATCAAAA 
GCATACCAAG CCTCAAACCT GGAACTGAAG AAAAAGCTGC AAAGTCAAGC TGAAGAAAAC 
AAAAAACGAA GCAAAAAGGC AGCAGAAAAA GCAGCAGGGA TACAAGATGA ATCCGCTGTA 
CCATTCCCTA CACCAAATGA ATCAGATAAC AAGAAAGGTC CAAAGAATGA AGACAATTCT 
ACAACAAACA CCAGTGGAAA AAACCTTGAG GTTCCACCAC GTATTGCCGT GACACGTCCA 
CCAGGACCAA CAGGACCTCC ATCTCCAAGA CCACCAGCTA TTCAAAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935639.1
CDS	1..2634
Translation	MGCYLMAIQNIVSDCKTLNGLTPAIGSRAEMPKHKKLVASENDVQIDVDNEEDNADCEEEEEEQPPKRREKNWRVPDMKPWLAEMTDDENRNANAEEPNENGEGSPGGRTGRKGRKGPGAAKRKPRDNSKKKKKKDADGSDSDEEAITEEELDKLKEAVEEKKKLIATIRSKPWKMKQKLEVLKDAQEFVEKFEGALGKGKGKKFYAYKVMMMKKWMKFQRDFENFRTACIPWEMKIKEIESHFGSSVASYFIFLRWMYGINLILFGLTFGLVMIPEALMGKPYGSMPRKTVPRPEQPTAMNFATLWDFSGFAQYSVLFYGYYNSQRTIGWLQFRLPLSYLLVGIGTIAYSFMVVIRTMAKNANDDGGGDDNSFNFSWKMYTSWDYLIGNPETADNKFASITTSFKESILEEQENRKEENIHLVRFLRVFANFLVMCTLAGSGYLIYFVVRRSQQFALEGLENYGWWERNEVNTVMSLLGMFCPTMFDVISNLENYHPRIALRWQLGRIFALFLGNLYTFLIALMDEINLKREEEKIVQHNMTFWEANIYNKSYSENSTVPFQVDPSDVPRGPCWETMVGQEFVRLTVSDIMTSYITILTGDFLQAVFVRFFNYCWCWDLEYGWPSYCEFDISGNVLGLIFNQGMIWMGSFYAPCLPAINVFRLHTSMYLQCWAVMCCNVPHARVFKASRSNNFYMAMLLFVLFLAMLPVVYTIVSIPPSFDCGPFSGKQRMFEVIQETLANDFPAWFAKVFSYASNPGLILPFILLMVMSIYYLNATSKAYQASNLELKKKLQSQAEENKKRSKKAAEKAAGIQDESAVPFPTPNESDNKKGPKNEDNSTTNTSGKNLEVPPRIAVTRPPGPTGPPSPRPPAIQRG

Target ORF information:

RefSeq Version	XM_002935593.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane channel like 1 (tmc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935593.1

ORF Insert Sequence:

ATGGGCTGTT ATCTGATGGC CATTCAGAAC ATCGTCTCTG ACTGCAAGAC CTTGAATGGT 
CTCACACCAG CTATCGGAAG CAGAGCTGAG ATGCCTAAAC ACAAAAAGCT TGTGGCCTCT 
GAAAATGACG TTCAAATTGA CGTGGACAAT GAAGAGGACA ATGCAGATTG TGAGGAGGAA 
GAAGAAGAAC AGCCTCCAAA GCGAAGGGAG AAAAACTGGA GAGTACCAGA CATGAAACCA 
TGGCTGGCAG AAATGACAGA TGATGAAAAC AGAAATGCCA ATGCAGAGGA GCCCAATGAG 
AATGGGGAAG GCAGCCCTGG GGGAAGGACT GGCAGAAAAG GAAGGAAGGG ACCTGGAGCA 
GCCAAGAGAA AGCCAAGGGA TAATTCCAAG AAGAAGAAAA AGAAAGATGC AGATGGATCT 
GACTCTGATG AAGAAGCCAT CACAGAAGAG GAATTAGACA AACTAAAGGA AGCCGTAGAA 
GAAAAAAAGA AACTCATTGC CACTATAAGA AGCAAACCTT GGAAAATGAA GCAAAAACTA 
GAAGTCCTCA AGGATGCGCA AGAGTTTGTG GAAAAATTTG AAGGAGCTTT GGGGAAAGGA 
AAAGGGAAGA AGTTTTACGC ATACAAGGTT ATGATGATGA AAAAATGGAT GAAGTTTCAA 
AGGGATTTTG AAAATTTCAG AACTGCCTGT ATACCCTGGG AGATGAAAAT CAAGGAAATT 
GAAAGTCATT TTGGTTCTTC AGTTGCTTCC TATTTTATAT TCCTACGCTG GATGTATGGG 
ATTAATTTGA TACTGTTTGG ACTAACCTTT GGCCTCGTCA TGATACCAGA GGCATTAATG 
GGAAAGCCAT ATGGTTCAAT GCCAAGAAAA ACTGTTCCAC GGCCTGAGCA GCCGACTGCA 
ATGAATTTTG CCACGCTTTG GGATTTCAGT GGATTTGCAC AATATTCTGT TCTTTTCTAT 
GGATACTACA ACAGTCAGAG AACAATTGGA TGGCTACAGT TTAGGCTGCC ACTTTCTTAC 
TTACTTGTTG GAATTGGTAC CATTGCATAC AGTTTCATGG TTGTTATCCG AACCATGGCC 
AAAAATGCTA ATGATGATGG AGGTGGTGAT GACAACAGCT TTAATTTTAG CTGGAAAATG 
TACACGAGTT GGGACTATCT TATTGGCAAC CCAGAAACAG CTGACAATAA ATTTGCATCA 
ATTACCACAA GCTTCAAGGA ATCGATTTTG GAAGAACAGG AAAACAGGAA AGAGGAAAAT 
ATTCATTTAG TTAGATTCCT CAGAGTCTTT GCAAACTTTC TTGTGATGTG CACTCTTGCA 
GGCAGTGGAT ACTTAATTTA CTTTGTTGTA AGGAGATCAC AACAATTTGC ATTGGAAGGT 
CTAGAAAACT ATGGATGGTG GGAACGGAAT GAAGTGAACA CAGTCATGTC ACTGCTAGGA 
ATGTTCTGCC CAACAATGTT TGATGTAATT AGTAATTTGG AGAATTATCA CCCAAGAATA 
GCACTGAGGT GGCAACTAGG ACGCATATTT GCTTTATTTT TGGGTAACCT GTATACATTT 
CTTATTGCCC TTATGGATGA AATCAATCTT AAGAGGGAAG AAGAGAAGAT TGTTCAACAT 
AATATGACAT TTTGGGAGGC AAATATTTAT AACAAATCCT ACTCTGAAAA TTCAACAGTC 
CCTTTCCAGG TAGATCCATC AGATGTTCCT CGAGGACCAT GTTGGGAAAC CATGGTGGGA 
CAGGAATTTG TTCGGCTTAC AGTTTCTGAT ATTATGACAT CTTATATTAC TATTCTAACT 
GGCGACTTCC TACAAGCTGT ATTTGTACGC TTTTTTAATT ACTGCTGGTG CTGGGATCTA 
GAATATGGAT GGCCATCGTA TTGCGAGTTC GACATCAGTG GCAATGTGCT GGGCCTCATT 
TTCAATCAAG GGATGATCTG GATGGGGTCA TTTTACGCTC CATGCCTGCC TGCTATTAAT 
GTATTTCGAC TTCACACATC CATGTACTTG CAGTGCTGGG CAGTAATGTG CTGCAATGTT 
CCCCATGCAC GAGTGTTTAA GGCATCCCGC TCCAATAACT TCTATATGGC TATGCTTCTT 
TTTGTTCTAT TTCTGGCCAT GTTACCAGTC GTCTATACCA TTGTCTCCAT TCCACCATCC 
TTTGACTGTG GCCCATTCAG TGGAAAACAA AGAATGTTTG AAGTCATACA AGAGACTCTG 
GCTAATGACT TCCCAGCCTG GTTTGCAAAA GTATTTAGCT ATGCATCAAA TCCCGGCCTT 
ATTCTACCTT TCATCCTCCT TATGGTAATG AGTATCTATT ACCTGAATGC TACATCAAAA 
GCATACCAAG CCTCAAACCT GGAACTGAAG AAAAAGCTGC AAAGTCAAGC TGAAGAAAAC 
AAAAAACGAA GCAAAAAGGC AGCAGAAAAA GCAGCAGGGA TACAAGATGA ATCCGCTGTA 
CCATTCCCTA CACCAAATGA ATCAGATAAC AAGAAAGGTC CAAAGAATGA AGACAATTCT 
ACAACAAACA CCAGTGGAAA AAACCTTGAG GTTCCACCAC GTATTGCCGT GACACGTCCA 
CCAGGACCAA CAGGACCTCC ATCTCCAAGA CCACCAGCTA TTCAAAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmc1 ( XM_002935593.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935639.1 Xenopus tropicalis transmembrane channel like 1 (tmc1), mRNA.

Price & Availability↑

CloneID	OXa05107
Clone ID Related Accession (Same CDS sequence)	XM_002935593.1 , XM_002935593.2
Accession Version	XM_002935593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transmembrane channel-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 15% of CDS bases ##RefSeq-Attributes-END##

ATGGGCTGTT ATCTGATGGC CATTCAGAAC ATCGTCTCTG ACTGCAAGAC CTTGAATGGT 
CTCACACCAG CTATCGGAAG CAGAGCTGAG ATGCCTAAAC ACAAAAAGCT TGTGGCCTCT 
GAAAATGACG TTCAAATTGA CGTGGACAAT GAAGAGGACA ATGCAGATTG TGAGGAGGAA 
GAAGAAGAAC AGCCTCCAAA GCGAAGGGAG AAAAACTGGA GAGTACCAGA CATGAAACCA 
TGGCTGGCAG AAATGACAGA TGATGAAAAC AGAAATGCCA ATGCAGAGGA GCCCAATGAG 
AATGGGGAAG GCAGCCCTGG GGGAAGGACT GGCAGAAAAG GAAGGAAGGG ACCTGGAGCA 
GCCAAGAGAA AGCCAAGGGA TAATTCCAAG AAGAAGAAAA AGAAAGATGC AGATGGATCT 
GACTCTGATG AAGAAGCCAT CACAGAAGAG GAATTAGACA AACTAAAGGA AGCCGTAGAA 
GAAAAAAAGA AACTCATTGC CACTATAAGA AGCAAACCTT GGAAAATGAA GCAAAAACTA 
GAAGTCCTCA AGGATGCGCA AGAGTTTGTG GAAAAATTTG AAGGAGCTTT GGGGAAAGGA 
AAAGGGAAGA AGTTTTACGC ATACAAGGTT ATGATGATGA AAAAATGGAT GAAGTTTCAA 
AGGGATTTTG AAAATTTCAG AACTGCCTGT ATACCCTGGG AGATGAAAAT CAAGGAAATT 
GAAAGTCATT TTGGTTCTTC AGTTGCTTCC TATTTTATAT TCCTACGCTG GATGTATGGG 
ATTAATTTGA TACTGTTTGG ACTAACCTTT GGCCTCGTCA TGATACCAGA GGCATTAATG 
GGAAAGCCAT ATGGTTCAAT GCCAAGAAAA ACTGTTCCAC GGCCTGAGCA GCCGACTGCA 
ATGAATTTTG CCACGCTTTG GGATTTCAGT GGATTTGCAC AATATTCTGT TCTTTTCTAT 
GGATACTACA ACAGTCAGAG AACAATTGGA TGGCTACAGT TTAGGCTGCC ACTTTCTTAC 
TTACTTGTTG GAATTGGTAC CATTGCATAC AGTTTCATGG TTGTTATCCG AACCATGGCC 
AAAAATGCTA ATGATGATGG AGGTGGTGAT GACAACAGCT TTAATTTTAG CTGGAAAATG 
TACACGAGTT GGGACTATCT TATTGGCAAC CCAGAAACAG CTGACAATAA ATTTGCATCA 
ATTACCACAA GCTTCAAGGA ATCGATTTTG GAAGAACAGG AAAACAGGAA AGAGGAAAAT 
ATTCATTTAG TTAGATTCCT CAGAGTCTTT GCAAACTTTC TTGTGATGTG CACTCTTGCA 
GGCAGTGGAT ACTTAATTTA CTTTGTTGTA AGGAGATCAC AACAATTTGC ATTGGAAGGT 
CTAGAAAACT ATGGATGGTG GGAACGGAAT GAAGTGAACA CAGTCATGTC ACTGCTAGGA 
ATGTTCTGCC CAACAATGTT TGATGTAATT AGTAATTTGG AGAATTATCA CCCAAGAATA 
GCACTGAGGT GGCAACTAGG ACGCATATTT GCTTTATTTT TGGGTAACCT GTATACATTT 
CTTATTGCCC TTATGGATGA AATCAATCTT AAGAGGGAAG AAGAGAAGAT TGTTCAACAT 
AATATGACAT TTTGGGAGGC AAATATTTAT AACAAATCCT ACTCTGAAAA TTCAACAGTC 
CCTTTCCAGG TAGATCCATC AGATGTTCCT CGAGGACCAT GTTGGGAAAC CATGGTGGGA 
CAGGAATTTG TTCGGCTTAC AGTTTCTGAT ATTATGACAT CTTATATTAC TATTCTAACT 
GGCGACTTCC TACAAGCTGT ATTTGTACGC TTTTTTAATT ACTGCTGGTG CTGGGATCTA 
GAATATGGAT GGCCATCGTA TTGCGAGTTC GACATCAGTG GCAATGTGCT GGGCCTCATT 
TTCAATCAAG GGATGATCTG GATGGGGTCA TTTTACGCTC CATGCCTGCC TGCTATTAAT 
GTATTTCGAC TTCACACATC CATGTACTTG CAGTGCTGGG CAGTAATGTG CTGCAATGTT 
CCCCATGCAC GAGTGTTTAA GGCATCCCGC TCCAATAACT TCTATATGGC TATGCTTCTT 
TTTGTTCTAT TTCTGGCCAT GTTACCAGTC GTCTATACCA TTGTCTCCAT TCCACCATCC 
TTTGACTGTG GCCCATTCAG TGGAAAACAA AGAATGTTTG AAGTCATACA AGAGACTCTG 
GCTAATGACT TCCCAGCCTG GTTTGCAAAA GTATTTAGCT ATGCATCAAA TCCCGGCCTT 
ATTCTACCTT TCATCCTCCT TATGGTAATG AGTATCTATT ACCTGAATGC TACATCAAAA 
GCATACCAAG CCTCAAACCT GGAACTGAAG AAAAAGCTGC AAAGTCAAGC TGAAGAAAAC 
AAAAAACGAA GCAAAAAGGC AGCAGAAAAA GCAGCAGGGA TACAAGATGA ATCCGCTGTA 
CCATTCCCTA CACCAAATGA ATCAGATAAC AAGAAAGGTC CAAAGAATGA AGACAATTCT 
ACAACAAACA CCAGTGGAAA AAACCTTGAG GTTCCACCAC GTATTGCCGT GACACGTCCA 
CCAGGACCAA CAGGACCTCC ATCTCCAAGA CCACCAGCTA TTCAAAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935639.1
CDS	1..2634
Translation	MGCYLMAIQNIVSDCKTLNGLTPAIGSRAEMPKHKKLVASENDVQIDVDNEEDNADCEEEEEEQPPKRREKNWRVPDMKPWLAEMTDDENRNANAEEPNENGEGSPGGRTGRKGRKGPGAAKRKPRDNSKKKKKKDADGSDSDEEAITEEELDKLKEAVEEKKKLIATIRSKPWKMKQKLEVLKDAQEFVEKFEGALGKGKGKKFYAYKVMMMKKWMKFQRDFENFRTACIPWEMKIKEIESHFGSSVASYFIFLRWMYGINLILFGLTFGLVMIPEALMGKPYGSMPRKTVPRPEQPTAMNFATLWDFSGFAQYSVLFYGYYNSQRTIGWLQFRLPLSYLLVGIGTIAYSFMVVIRTMAKNANDDGGGDDNSFNFSWKMYTSWDYLIGNPETADNKFASITTSFKESILEEQENRKEENIHLVRFLRVFANFLVMCTLAGSGYLIYFVVRRSQQFALEGLENYGWWERNEVNTVMSLLGMFCPTMFDVISNLENYHPRIALRWQLGRIFALFLGNLYTFLIALMDEINLKREEEKIVQHNMTFWEANIYNKSYSENSTVPFQVDPSDVPRGPCWETMVGQEFVRLTVSDIMTSYITILTGDFLQAVFVRFFNYCWCWDLEYGWPSYCEFDISGNVLGLIFNQGMIWMGSFYAPCLPAINVFRLHTSMYLQCWAVMCCNVPHARVFKASRSNNFYMAMLLFVLFLAMLPVVYTIVSIPPSFDCGPFSGKQRMFEVIQETLANDFPAWFAKVFSYASNPGLILPFILLMVMSIYYLNATSKAYQASNLELKKKLQSQAEENKKRSKKAAEKAAGIQDESAVPFPTPNESDNKKGPKNEDNSTTNTSGKNLEVPPRIAVTRPPGPTGPPSPRPPAIQRG

Target ORF information:

RefSeq Version	XM_002935593.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane channel like 1 (tmc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935593.2

ORF Insert Sequence:

ATGGGCTGTT ATCTGATGGC CATTCAGAAC ATCGTCTCTG ACTGCAAGAC CTTGAATGGT 
CTCACACCAG CTATCGGAAG CAGAGCTGAG ATGCCTAAAC ACAAAAAGCT TGTGGCCTCT 
GAAAATGACG TTCAAATTGA CGTGGACAAT GAAGAGGACA ATGCAGATTG TGAGGAGGAA 
GAAGAAGAAC AGCCTCCAAA GCGAAGGGAG AAAAACTGGA GAGTACCAGA CATGAAACCA 
TGGCTGGCAG AAATGACAGA TGATGAAAAC AGAAATGCCA ATGCAGAGGA GCCCAATGAG 
AATGGGGAAG GCAGCCCTGG GGGAAGGACT GGCAGAAAAG GAAGGAAGGG ACCTGGAGCA 
GCCAAGAGAA AGCCAAGGGA TAATTCCAAG AAGAAGAAAA AGAAAGATGC AGATGGATCT 
GACTCTGATG AAGAAGCCAT CACAGAAGAG GAATTAGACA AACTAAAGGA AGCCGTAGAA 
GAAAAAAAGA AACTCATTGC CACTATAAGA AGCAAACCTT GGAAAATGAA GCAAAAACTA 
GAAGTCCTCA AGGATGCGCA AGAGTTTGTG GAAAAATTTG AAGGAGCTTT GGGGAAAGGA 
AAAGGGAAGA AGTTTTACGC ATACAAGGTT ATGATGATGA AAAAATGGAT GAAGTTTCAA 
AGGGATTTTG AAAATTTCAG AACTGCCTGT ATACCCTGGG AGATGAAAAT CAAGGAAATT 
GAAAGTCATT TTGGTTCTTC AGTTGCTTCC TATTTTATAT TCCTACGCTG GATGTATGGG 
ATTAATTTGA TACTGTTTGG ACTAACCTTT GGCCTCGTCA TGATACCAGA GGCATTAATG 
GGAAAGCCAT ATGGTTCAAT GCCAAGAAAA ACTGTTCCAC GGCCTGAGCA GCCGACTGCA 
ATGAATTTTG CCACGCTTTG GGATTTCAGT GGATTTGCAC AATATTCTGT TCTTTTCTAT 
GGATACTACA ACAGTCAGAG AACAATTGGA TGGCTACAGT TTAGGCTGCC ACTTTCTTAC 
TTACTTGTTG GAATTGGTAC CATTGCATAC AGTTTCATGG TTGTTATCCG AACCATGGCC 
AAAAATGCTA ATGATGATGG AGGTGGTGAT GACAACAGCT TTAATTTTAG CTGGAAAATG 
TACACGAGTT GGGACTATCT TATTGGCAAC CCAGAAACAG CTGACAATAA ATTTGCATCA 
ATTACCACAA GCTTCAAGGA ATCGATTTTG GAAGAACAGG AAAACAGGAA AGAGGAAAAT 
ATTCATTTAG TTAGATTCCT CAGAGTCTTT GCAAACTTTC TTGTGATGTG CACTCTTGCA 
GGCAGTGGAT ACTTAATTTA CTTTGTTGTA AGGAGATCAC AACAATTTGC ATTGGAAGGT 
CTAGAAAACT ATGGATGGTG GGAACGGAAT GAAGTGAACA CAGTCATGTC ACTGCTAGGA 
ATGTTCTGCC CAACAATGTT TGATGTAATT AGTAATTTGG AGAATTATCA CCCAAGAATA 
GCACTGAGGT GGCAACTAGG ACGCATATTT GCTTTATTTT TGGGTAACCT GTATACATTT 
CTTATTGCCC TTATGGATGA AATCAATCTT AAGAGGGAAG AAGAGAAGAT TGTTCAACAT 
AATATGACAT TTTGGGAGGC AAATATTTAT AACAAATCCT ACTCTGAAAA TTCAACAGTC 
CCTTTCCAGG TAGATCCATC AGATGTTCCT CGAGGACCAT GTTGGGAAAC CATGGTGGGA 
CAGGAATTTG TTCGGCTTAC AGTTTCTGAT ATTATGACAT CTTATATTAC TATTCTAACT 
GGCGACTTCC TACAAGCTGT ATTTGTACGC TTTTTTAATT ACTGCTGGTG CTGGGATCTA 
GAATATGGAT GGCCATCGTA TTGCGAGTTC GACATCAGTG GCAATGTGCT GGGCCTCATT 
TTCAATCAAG GGATGATCTG GATGGGGTCA TTTTACGCTC CATGCCTGCC TGCTATTAAT 
GTATTTCGAC TTCACACATC CATGTACTTG CAGTGCTGGG CAGTAATGTG CTGCAATGTT 
CCCCATGCAC GAGTGTTTAA GGCATCCCGC TCCAATAACT TCTATATGGC TATGCTTCTT 
TTTGTTCTAT TTCTGGCCAT GTTACCAGTC GTCTATACCA TTGTCTCCAT TCCACCATCC 
TTTGACTGTG GCCCATTCAG TGGAAAACAA AGAATGTTTG AAGTCATACA AGAGACTCTG 
GCTAATGACT TCCCAGCCTG GTTTGCAAAA GTATTTAGCT ATGCATCAAA TCCCGGCCTT 
ATTCTACCTT TCATCCTCCT TATGGTAATG AGTATCTATT ACCTGAATGC TACATCAAAA 
GCATACCAAG CCTCAAACCT GGAACTGAAG AAAAAGCTGC AAAGTCAAGC TGAAGAAAAC 
AAAAAACGAA GCAAAAAGGC AGCAGAAAAA GCAGCAGGGA TACAAGATGA ATCCGCTGTA 
CCATTCCCTA CACCAAATGA ATCAGATAAC AAGAAAGGTC CAAAGAATGA AGACAATTCT 
ACAACAAACA CCAGTGGAAA AAACCTTGAG GTTCCACCAC GTATTGCCGT GACACGTCCA 
CCAGGACCAA CAGGACCTCC ATCTCCAAGA CCACCAGCTA TTCAAAGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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tmc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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