LOC100241404 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100241404
Entrez Gene ID	100241404
Full Name	TMV resistance protein N
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019216195.1	XP_019071740.1	disease resistance protein TAO1 isoform X2
XM_019216194.1	XP_019071739.1	TMV resistance protein N isoform X1
XM_010645725.2	XP_010644027.1	TMV resistance protein N isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100241404 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100241404 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OVa23285	XM_019216194.1 Latest version!	Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OVa23285	XM_010645725.2 Latest version!	Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OVa43230	XM_019216195.1 Latest version!	Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100241404 ( XM_019216194.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019071739.1 Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OVa23285
Clone ID Related Accession (Same CDS sequence)	XM_019216194.1 , XM_010645725.2
Accession Version	XM_019216194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	TMV resistance protein N isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012024.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA CAAGCGTTCA AATGGCGTCT GATTATTCTT CTTCCACCCC TAGATGCACG 
TATGACGTGT TCTTGAGTTT TAGAGGGGAA GACACCCGCA ACAATTTCAC CGATCACCTG 
TATACTGCTT TGGTTCAAAG GGGCATCAAT ACTTTTAGAG ATGATAAACT TAGAAGAGGA 
GAAGAGATTG CACCAGAGCT TCTAAAAGCT ATTGAAGAAT CAAGATCTTC CATTGTTGTT 
TTCTCAAAAA CTTATGCTCA TTCAAGGTGG TGTTTAGACG AGCTTGCAAA GATCATGGAG 
TGTAGACGTG AATATAGGCA AATAGTATTG CCAATTTTCT ACCATGTGGA TCCAGCGGAT 
GTGCGTAAAC AAACAGGGAG TTTTGGAGAA GCATTCACCA GCTATGAAGA AAATTGGAAG 
AACAAGGCAC AAAGGTGGAG GGAGGCTTTG ACCGAAGCAG GCTATATTGC TGGATGGCCT 
ATAAACAAGG GGTACGAGTC AAGGCCTATT GAGGAAATTA TTAACCACAT CCTCAAAAGA 
TTGAATCCTA AGTTTTTGCC TATTAAGGAG CACATGGTTG GAATGTATGT TCACTTGGAA 
GAACTAAAAT CATTGTTAAA AATGCAGTTG GATGATGTTC GCATGGTTGG GATCTATGGA 
ATTGGTGGAA TTGGCAAAAC TACTATTGCC AAGATGGTTT ATAATGACAT CCTATGTCAA 
TTCAATGGTG CTAGCTTTCT TGAAGGTGTC AAAAATAGAT CCAAATGTTA CAATGATCAA 
CTTCAATTAC TGCAAGAACT TCTTCATGGC ATTATGGAGG GTGGACATTT AAAATTAGAA 
AGTATTAATG ATGGAATGAA TATGATCAAG GGCAGACTTG GCTCAAAAAA GGTTCTTGTT 
GTTTTTGATG ATGTCGATGA TTTGGACCAA GTACGAGGAA TAGTTGCAAA CTATAAATGG 
TTTGGTGGAG GAAGCAGAAT CATCATAACT ACCAGAGACA AACATTTGCT AGATCAATAT 
GAGGTGCATG CATCATATGA GGCTAAGGTG TTATGTTATG AAGATGCTAT TGAACTCTTT 
AGTTGGCATG CCTTCAAAGT ACAAAACATA CGTGAAGATT ATGTAGAGAT GTCAAATAGT 
ATGATAAAAT ATGCGCAAGG CCTTCCTTTA GCCCTTGAAG TTTTGGGCTC TTCTCTTTAT 
AACAAGACAA AAGATGAATG GAAAAGTGCA ATAGAGAAAT TGAAAAAAAA CCCTAACAAG 
AAAATTAATG ATGTGCTTAA AATAAGCCTT GATGGGCTTG ATCGTACTCA AAGGGAGATT 
TTCCTTCATA TCGCATGCTT TTTCAAGGGA GAAGCTAAAG ATTTTATATT GAGAATATTA 
GATGATCATG CAGAATATGA CATAGGAGTT CTTTGTGATA GATGCCTAAT AACTATTTCC 
TACAACAAGG TAGAAATGCA TGACTTGATA CAACAAATGG GTTGGACAAT TGATCGTGAA 
AAACATCTTA AAGACCCAAG CAAATGGATC AGATTGTGGG ATCCAGATGA TATTTCTAAA 
GCATTTTCTG CACAAGAGGG AATGGAACAA GTTGAAGTCA TATCATATGA CTTGTCTCGA 
TCAAAAGAAA TGCAGGTGAA TAAAAAGGTT TATGAAAATA TGAAGGAACT AAGATTTCTC 
AAACTCCATT GGGGGGATCA TCATGGTTTT ACGACGAAGA AATATAAAGT GTTCCTTCCC 
AAAGACTTTG AGTTTCCTTC TCAAAAGTTA AGATATCTTT ATTGGGATGG ATACCCTTTG 
CAAACCTTGC CATCAAAGTT TAATGGAGAG AATCTTGTTG AGCTCCACAT GAAAAATAGC 
ACCATAAAAC AACTTTGGAA TGGGCGTAAG ATTCTAGGAA ATTTAAAGAT CATTGACTTA 
AGTAGATCAC GATTGCTCAC CAAAATGCCA GAATTATCAA GCATGCCAAA TCTTGAGGAG 
CTTAACCTCG TGTGTTGTGA AAGGTTGAAG AAATTTCCTG AGATCCGTGA AAATATGGGA 
CGTTTGGAGA GAGTTCATTT AGATTGCAGT GGAATTCAGG AAATACCGAG CAGTATTGAG 
TACTTGCCAG CTCTTGAATT TCTTACACTT CATTATTGCA GAAATTTTGA CAAATTTCCG 
GATAATTTTG GGAATTTGAG ACATCTAAGG GTCATCAATG CAAACAGGAC TGATATTAAA 
GAACTTCCTG AGATCCATAA TATGGGAAGT CTAACGAAAC TTTTTCTAAT CGAGACTGCT 
ATTAAAGAAT TACCACGCTC AATAGGTCAT CTCACCGAAC TTGAGGAGTT AAATCTAGAA 
AATTGCAAAA ATTTGAGGAG TCTTCCTAAT AGCATTTGTG GGTTGAAATC ACTTGGAGTC 
CTCAATCTCA ATGGCTGTTC AAATCTGGTG GCTTTTCCAG AAATCATGGA GGATATGGAA 
GACTTAAGAG AACTTCTTTT AAGTAAAACG CCTATAACAG AGCTGCCACC ATCAATTGAA 
CATCTAAAAG GTCTTGAACA CTTGGAATTG AAGAATTGTG AGAACCTTGT GACTCTTCCA 
GATAGCATTG GTAATTTGAC ACATCTTCGT AGTCTTTGTG TTCGTAACTG TTCGAAGCTC 
CACAATTTGC CCGACAATCT GAGAAGCTTG CAATGGTGCT TAAGAAGACT GGATCTAGCT 
GGTTGCAATC TGATGAAAGG AGCAATTCCT AGTGATTTAT GGTGCCTATC CTTACTGAGA 
TTTTTAGATG TGAGCGAAAT CCCTATTCCT TGCATACCTA CTAACATAAT CCAACTCTCT 
AACCTGAGAA CGCTTAGGAT GAATCACTGC CAGATGCTTG AAGAAATTCC AGAGCTTCCA 
TCAAGGCTAG AAATCTTAGA AGCACAAGGT TGTCCACATC TGGGAACTTT ATCAACTCCT 
TCGAGCCCAC TCTGGTCTTA TCTCCTCAAC CTCTTCAAAT CACGTACTCA GAGTTGTGAA 
TACGAAATAG ACTCAGACTC CTTATGGTAC TTCCATGTAC CTAAAGTTGT TATTCCAGGA 
AGTGGTGGAA TACCAAAGTG GATAAGTCAT CCGAGTATGG GACGCCAAGC AATAATAGAG 
CTCCCAAAGA ATCGGTATGA AGATAACAAT TTCTTGGGAT TTGCTGTTTT CTTCCATCAT 
GTTCCACTTG ATGATTTTTG GTCGCATTGG CATAGGCGTT TTCTACAATT TGAATTGAGG 
ATATCCCATG ATGATCAATC TGAACGGGTA ATTAAAATAA TGAGTTGTTT CCATTTTGAA 
ACATGCCGTA GTAGTAGTTC ATCATCTGAG GGTATATGCC TCGACAATGT TAGCACATCG 
GATCCACCAT TGTGCGTGAC CTATCTCCCT AAGTTTGCCA TTCCAAGTGA GCATCGATAC 
GCTAAGCGTA GTCAACGTGA TACTATCCCA GTCGTTGAAG TGGAAATTTG TGGGATACAT 
CTTATATACT CCAAAAATGA TCACCAACAG AATCAGAGCC ATTGGCGTGA AGCCATGATA 
AAAGATCAAG ACATCTCCTG TGATTTCCAA GCTTCTCCCT ACAGGTCCTT TCGGATGAAA 
GAAATCCCCA AGTGGATCAA CGCGTGGAGG TCTAGTGAAA GCGACAGCTC TAATACAGAA 
GGCTCAGAAT CAGAGAACTC TGATTACTAT TCTGCTGACG AGGGGGACCC CACTGTTGAC 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019071739.1
CDS	230..3955
Translation	MASTSVQMASDYSSSTPRCTYDVFLSFRGEDTRNNFTDHLYTALVQRGINTFRDDKLRRGEEIAPELLKAIEESRSSIVVFSKTYAHSRWCLDELAKIMECRREYRQIVLPIFYHVDPADVRKQTGSFGEAFTSYEENWKNKAQRWREALTEAGYIAGWPINKGYESRPIEEIINHILKRLNPKFLPIKEHMVGMYVHLEELKSLLKMQLDDVRMVGIYGIGGIGKTTIAKMVYNDILCQFNGASFLEGVKNRSKCYNDQLQLLQELLHGIMEGGHLKLESINDGMNMIKGRLGSKKVLVVFDDVDDLDQVRGIVANYKWFGGGSRIIITTRDKHLLDQYEVHASYEAKVLCYEDAIELFSWHAFKVQNIREDYVEMSNSMIKYAQGLPLALEVLGSSLYNKTKDEWKSAIEKLKKNPNKKINDVLKISLDGLDRTQREIFLHIACFFKGEAKDFILRILDDHAEYDIGVLCDRCLITISYNKVEMHDLIQQMGWTIDREKHLKDPSKWIRLWDPDDISKAFSAQEGMEQVEVISYDLSRSKEMQVNKKVYENMKELRFLKLHWGDHHGFTTKKYKVFLPKDFEFPSQKLRYLYWDGYPLQTLPSKFNGENLVELHMKNSTIKQLWNGRKILGNLKIIDLSRSRLLTKMPELSSMPNLEELNLVCCERLKKFPEIRENMGRLERVHLDCSGIQEIPSSIEYLPALEFLTLHYCRNFDKFPDNFGNLRHLRVINANRTDIKELPEIHNMGSLTKLFLIETAIKELPRSIGHLTELEELNLENCKNLRSLPNSICGLKSLGVLNLNGCSNLVAFPEIMEDMEDLRELLLSKTPITELPPSIEHLKGLEHLELKNCENLVTLPDSIGNLTHLRSLCVRNCSKLHNLPDNLRSLQWCLRRLDLAGCNLMKGAIPSDLWCLSLLRFLDVSEIPIPCIPTNIIQLSNLRTLRMNHCQMLEEIPELPSRLEILEAQGCPHLGTLSTPSSPLWSYLLNLFKSRTQSCEYEIDSDSLWYFHVPKVVIPGSGGIPKWISHPSMGRQAIIELPKNRYEDNNFLGFAVFFHHVPLDDFWSHWHRRFLQFELRISHDDQSERVIKIMSCFHFETCRSSSSSSEGICLDNVSTSDPPLCVTYLPKFAIPSEHRYAKRSQRDTIPVVEVEICGIHLIYSKNDHQQNQSHWREAMIKDQDISCDFQASPYRSFRMKEIPKWINAWRSSESDSSNTEGSESENSDYYSADEGDPTVDC

Target ORF information:

RefSeq Version	XM_019216194.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019216194.1

ORF Insert Sequence:

ATGGCTTCCA CAAGCGTTCA AATGGCGTCT GATTATTCTT CTTCCACCCC TAGATGCACG 
TATGACGTGT TCTTGAGTTT TAGAGGGGAA GACACCCGCA ACAATTTCAC CGATCACCTG 
TATACTGCTT TGGTTCAAAG GGGCATCAAT ACTTTTAGAG ATGATAAACT TAGAAGAGGA 
GAAGAGATTG CACCAGAGCT TCTAAAAGCT ATTGAAGAAT CAAGATCTTC CATTGTTGTT 
TTCTCAAAAA CTTATGCTCA TTCAAGGTGG TGTTTAGACG AGCTTGCAAA GATCATGGAG 
TGTAGACGTG AATATAGGCA AATAGTATTG CCAATTTTCT ACCATGTGGA TCCAGCGGAT 
GTGCGTAAAC AAACAGGGAG TTTTGGAGAA GCATTCACCA GCTATGAAGA AAATTGGAAG 
AACAAGGCAC AAAGGTGGAG GGAGGCTTTG ACCGAAGCAG GCTATATTGC TGGATGGCCT 
ATAAACAAGG GGTACGAGTC AAGGCCTATT GAGGAAATTA TTAACCACAT CCTCAAAAGA 
TTGAATCCTA AGTTTTTGCC TATTAAGGAG CACATGGTTG GAATGTATGT TCACTTGGAA 
GAACTAAAAT CATTGTTAAA AATGCAGTTG GATGATGTTC GCATGGTTGG GATCTATGGA 
ATTGGTGGAA TTGGCAAAAC TACTATTGCC AAGATGGTTT ATAATGACAT CCTATGTCAA 
TTCAATGGTG CTAGCTTTCT TGAAGGTGTC AAAAATAGAT CCAAATGTTA CAATGATCAA 
CTTCAATTAC TGCAAGAACT TCTTCATGGC ATTATGGAGG GTGGACATTT AAAATTAGAA 
AGTATTAATG ATGGAATGAA TATGATCAAG GGCAGACTTG GCTCAAAAAA GGTTCTTGTT 
GTTTTTGATG ATGTCGATGA TTTGGACCAA GTACGAGGAA TAGTTGCAAA CTATAAATGG 
TTTGGTGGAG GAAGCAGAAT CATCATAACT ACCAGAGACA AACATTTGCT AGATCAATAT 
GAGGTGCATG CATCATATGA GGCTAAGGTG TTATGTTATG AAGATGCTAT TGAACTCTTT 
AGTTGGCATG CCTTCAAAGT ACAAAACATA CGTGAAGATT ATGTAGAGAT GTCAAATAGT 
ATGATAAAAT ATGCGCAAGG CCTTCCTTTA GCCCTTGAAG TTTTGGGCTC TTCTCTTTAT 
AACAAGACAA AAGATGAATG GAAAAGTGCA ATAGAGAAAT TGAAAAAAAA CCCTAACAAG 
AAAATTAATG ATGTGCTTAA AATAAGCCTT GATGGGCTTG ATCGTACTCA AAGGGAGATT 
TTCCTTCATA TCGCATGCTT TTTCAAGGGA GAAGCTAAAG ATTTTATATT GAGAATATTA 
GATGATCATG CAGAATATGA CATAGGAGTT CTTTGTGATA GATGCCTAAT AACTATTTCC 
TACAACAAGG TAGAAATGCA TGACTTGATA CAACAAATGG GTTGGACAAT TGATCGTGAA 
AAACATCTTA AAGACCCAAG CAAATGGATC AGATTGTGGG ATCCAGATGA TATTTCTAAA 
GCATTTTCTG CACAAGAGGG AATGGAACAA GTTGAAGTCA TATCATATGA CTTGTCTCGA 
TCAAAAGAAA TGCAGGTGAA TAAAAAGGTT TATGAAAATA TGAAGGAACT AAGATTTCTC 
AAACTCCATT GGGGGGATCA TCATGGTTTT ACGACGAAGA AATATAAAGT GTTCCTTCCC 
AAAGACTTTG AGTTTCCTTC TCAAAAGTTA AGATATCTTT ATTGGGATGG ATACCCTTTG 
CAAACCTTGC CATCAAAGTT TAATGGAGAG AATCTTGTTG AGCTCCACAT GAAAAATAGC 
ACCATAAAAC AACTTTGGAA TGGGCGTAAG ATTCTAGGAA ATTTAAAGAT CATTGACTTA 
AGTAGATCAC GATTGCTCAC CAAAATGCCA GAATTATCAA GCATGCCAAA TCTTGAGGAG 
CTTAACCTCG TGTGTTGTGA AAGGTTGAAG AAATTTCCTG AGATCCGTGA AAATATGGGA 
CGTTTGGAGA GAGTTCATTT AGATTGCAGT GGAATTCAGG AAATACCGAG CAGTATTGAG 
TACTTGCCAG CTCTTGAATT TCTTACACTT CATTATTGCA GAAATTTTGA CAAATTTCCG 
GATAATTTTG GGAATTTGAG ACATCTAAGG GTCATCAATG CAAACAGGAC TGATATTAAA 
GAACTTCCTG AGATCCATAA TATGGGAAGT CTAACGAAAC TTTTTCTAAT CGAGACTGCT 
ATTAAAGAAT TACCACGCTC AATAGGTCAT CTCACCGAAC TTGAGGAGTT AAATCTAGAA 
AATTGCAAAA ATTTGAGGAG TCTTCCTAAT AGCATTTGTG GGTTGAAATC ACTTGGAGTC 
CTCAATCTCA ATGGCTGTTC AAATCTGGTG GCTTTTCCAG AAATCATGGA GGATATGGAA 
GACTTAAGAG AACTTCTTTT AAGTAAAACG CCTATAACAG AGCTGCCACC ATCAATTGAA 
CATCTAAAAG GTCTTGAACA CTTGGAATTG AAGAATTGTG AGAACCTTGT GACTCTTCCA 
GATAGCATTG GTAATTTGAC ACATCTTCGT AGTCTTTGTG TTCGTAACTG TTCGAAGCTC 
CACAATTTGC CCGACAATCT GAGAAGCTTG CAATGGTGCT TAAGAAGACT GGATCTAGCT 
GGTTGCAATC TGATGAAAGG AGCAATTCCT AGTGATTTAT GGTGCCTATC CTTACTGAGA 
TTTTTAGATG TGAGCGAAAT CCCTATTCCT TGCATACCTA CTAACATAAT CCAACTCTCT 
AACCTGAGAA CGCTTAGGAT GAATCACTGC CAGATGCTTG AAGAAATTCC AGAGCTTCCA 
TCAAGGCTAG AAATCTTAGA AGCACAAGGT TGTCCACATC TGGGAACTTT ATCAACTCCT 
TCGAGCCCAC TCTGGTCTTA TCTCCTCAAC CTCTTCAAAT CACGTACTCA GAGTTGTGAA 
TACGAAATAG ACTCAGACTC CTTATGGTAC TTCCATGTAC CTAAAGTTGT TATTCCAGGA 
AGTGGTGGAA TACCAAAGTG GATAAGTCAT CCGAGTATGG GACGCCAAGC AATAATAGAG 
CTCCCAAAGA ATCGGTATGA AGATAACAAT TTCTTGGGAT TTGCTGTTTT CTTCCATCAT 
GTTCCACTTG ATGATTTTTG GTCGCATTGG CATAGGCGTT TTCTACAATT TGAATTGAGG 
ATATCCCATG ATGATCAATC TGAACGGGTA ATTAAAATAA TGAGTTGTTT CCATTTTGAA 
ACATGCCGTA GTAGTAGTTC ATCATCTGAG GGTATATGCC TCGACAATGT TAGCACATCG 
GATCCACCAT TGTGCGTGAC CTATCTCCCT AAGTTTGCCA TTCCAAGTGA GCATCGATAC 
GCTAAGCGTA GTCAACGTGA TACTATCCCA GTCGTTGAAG TGGAAATTTG TGGGATACAT 
CTTATATACT CCAAAAATGA TCACCAACAG AATCAGAGCC ATTGGCGTGA AGCCATGATA 
AAAGATCAAG ACATCTCCTG TGATTTCCAA GCTTCTCCCT ACAGGTCCTT TCGGATGAAA 
GAAATCCCCA AGTGGATCAA CGCGTGGAGG TCTAGTGAAA GCGACAGCTC TAATACAGAA 
GGCTCAGAAT CAGAGAACTC TGATTACTAT TCTGCTGACG AGGGGGACCC CACTGTTGAC 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100241404 ( XM_010645725.2 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_010644027.1 Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OVa23285
Clone ID Related Accession (Same CDS sequence)	XM_019216194.1 , XM_010645725.2
Accession Version	XM_010645725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	TMV resistance protein N isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012024.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2016 this sequence version replaced XM_010645725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA CAAGCGTTCA AATGGCGTCT GATTATTCTT CTTCCACCCC TAGATGCACG 
TATGACGTGT TCTTGAGTTT TAGAGGGGAA GACACCCGCA ACAATTTCAC CGATCACCTG 
TATACTGCTT TGGTTCAAAG GGGCATCAAT ACTTTTAGAG ATGATAAACT TAGAAGAGGA 
GAAGAGATTG CACCAGAGCT TCTAAAAGCT ATTGAAGAAT CAAGATCTTC CATTGTTGTT 
TTCTCAAAAA CTTATGCTCA TTCAAGGTGG TGTTTAGACG AGCTTGCAAA GATCATGGAG 
TGTAGACGTG AATATAGGCA AATAGTATTG CCAATTTTCT ACCATGTGGA TCCAGCGGAT 
GTGCGTAAAC AAACAGGGAG TTTTGGAGAA GCATTCACCA GCTATGAAGA AAATTGGAAG 
AACAAGGCAC AAAGGTGGAG GGAGGCTTTG ACCGAAGCAG GCTATATTGC TGGATGGCCT 
ATAAACAAGG GGTACGAGTC AAGGCCTATT GAGGAAATTA TTAACCACAT CCTCAAAAGA 
TTGAATCCTA AGTTTTTGCC TATTAAGGAG CACATGGTTG GAATGTATGT TCACTTGGAA 
GAACTAAAAT CATTGTTAAA AATGCAGTTG GATGATGTTC GCATGGTTGG GATCTATGGA 
ATTGGTGGAA TTGGCAAAAC TACTATTGCC AAGATGGTTT ATAATGACAT CCTATGTCAA 
TTCAATGGTG CTAGCTTTCT TGAAGGTGTC AAAAATAGAT CCAAATGTTA CAATGATCAA 
CTTCAATTAC TGCAAGAACT TCTTCATGGC ATTATGGAGG GTGGACATTT AAAATTAGAA 
AGTATTAATG ATGGAATGAA TATGATCAAG GGCAGACTTG GCTCAAAAAA GGTTCTTGTT 
GTTTTTGATG ATGTCGATGA TTTGGACCAA GTACGAGGAA TAGTTGCAAA CTATAAATGG 
TTTGGTGGAG GAAGCAGAAT CATCATAACT ACCAGAGACA AACATTTGCT AGATCAATAT 
GAGGTGCATG CATCATATGA GGCTAAGGTG TTATGTTATG AAGATGCTAT TGAACTCTTT 
AGTTGGCATG CCTTCAAAGT ACAAAACATA CGTGAAGATT ATGTAGAGAT GTCAAATAGT 
ATGATAAAAT ATGCGCAAGG CCTTCCTTTA GCCCTTGAAG TTTTGGGCTC TTCTCTTTAT 
AACAAGACAA AAGATGAATG GAAAAGTGCA ATAGAGAAAT TGAAAAAAAA CCCTAACAAG 
AAAATTAATG ATGTGCTTAA AATAAGCCTT GATGGGCTTG ATCGTACTCA AAGGGAGATT 
TTCCTTCATA TCGCATGCTT TTTCAAGGGA GAAGCTAAAG ATTTTATATT GAGAATATTA 
GATGATCATG CAGAATATGA CATAGGAGTT CTTTGTGATA GATGCCTAAT AACTATTTCC 
TACAACAAGG TAGAAATGCA TGACTTGATA CAACAAATGG GTTGGACAAT TGATCGTGAA 
AAACATCTTA AAGACCCAAG CAAATGGATC AGATTGTGGG ATCCAGATGA TATTTCTAAA 
GCATTTTCTG CACAAGAGGG AATGGAACAA GTTGAAGTCA TATCATATGA CTTGTCTCGA 
TCAAAAGAAA TGCAGGTGAA TAAAAAGGTT TATGAAAATA TGAAGGAACT AAGATTTCTC 
AAACTCCATT GGGGGGATCA TCATGGTTTT ACGACGAAGA AATATAAAGT GTTCCTTCCC 
AAAGACTTTG AGTTTCCTTC TCAAAAGTTA AGATATCTTT ATTGGGATGG ATACCCTTTG 
CAAACCTTGC CATCAAAGTT TAATGGAGAG AATCTTGTTG AGCTCCACAT GAAAAATAGC 
ACCATAAAAC AACTTTGGAA TGGGCGTAAG ATTCTAGGAA ATTTAAAGAT CATTGACTTA 
AGTAGATCAC GATTGCTCAC CAAAATGCCA GAATTATCAA GCATGCCAAA TCTTGAGGAG 
CTTAACCTCG TGTGTTGTGA AAGGTTGAAG AAATTTCCTG AGATCCGTGA AAATATGGGA 
CGTTTGGAGA GAGTTCATTT AGATTGCAGT GGAATTCAGG AAATACCGAG CAGTATTGAG 
TACTTGCCAG CTCTTGAATT TCTTACACTT CATTATTGCA GAAATTTTGA CAAATTTCCG 
GATAATTTTG GGAATTTGAG ACATCTAAGG GTCATCAATG CAAACAGGAC TGATATTAAA 
GAACTTCCTG AGATCCATAA TATGGGAAGT CTAACGAAAC TTTTTCTAAT CGAGACTGCT 
ATTAAAGAAT TACCACGCTC AATAGGTCAT CTCACCGAAC TTGAGGAGTT AAATCTAGAA 
AATTGCAAAA ATTTGAGGAG TCTTCCTAAT AGCATTTGTG GGTTGAAATC ACTTGGAGTC 
CTCAATCTCA ATGGCTGTTC AAATCTGGTG GCTTTTCCAG AAATCATGGA GGATATGGAA 
GACTTAAGAG AACTTCTTTT AAGTAAAACG CCTATAACAG AGCTGCCACC ATCAATTGAA 
CATCTAAAAG GTCTTGAACA CTTGGAATTG AAGAATTGTG AGAACCTTGT GACTCTTCCA 
GATAGCATTG GTAATTTGAC ACATCTTCGT AGTCTTTGTG TTCGTAACTG TTCGAAGCTC 
CACAATTTGC CCGACAATCT GAGAAGCTTG CAATGGTGCT TAAGAAGACT GGATCTAGCT 
GGTTGCAATC TGATGAAAGG AGCAATTCCT AGTGATTTAT GGTGCCTATC CTTACTGAGA 
TTTTTAGATG TGAGCGAAAT CCCTATTCCT TGCATACCTA CTAACATAAT CCAACTCTCT 
AACCTGAGAA CGCTTAGGAT GAATCACTGC CAGATGCTTG AAGAAATTCC AGAGCTTCCA 
TCAAGGCTAG AAATCTTAGA AGCACAAGGT TGTCCACATC TGGGAACTTT ATCAACTCCT 
TCGAGCCCAC TCTGGTCTTA TCTCCTCAAC CTCTTCAAAT CACGTACTCA GAGTTGTGAA 
TACGAAATAG ACTCAGACTC CTTATGGTAC TTCCATGTAC CTAAAGTTGT TATTCCAGGA 
AGTGGTGGAA TACCAAAGTG GATAAGTCAT CCGAGTATGG GACGCCAAGC AATAATAGAG 
CTCCCAAAGA ATCGGTATGA AGATAACAAT TTCTTGGGAT TTGCTGTTTT CTTCCATCAT 
GTTCCACTTG ATGATTTTTG GTCGCATTGG CATAGGCGTT TTCTACAATT TGAATTGAGG 
ATATCCCATG ATGATCAATC TGAACGGGTA ATTAAAATAA TGAGTTGTTT CCATTTTGAA 
ACATGCCGTA GTAGTAGTTC ATCATCTGAG GGTATATGCC TCGACAATGT TAGCACATCG 
GATCCACCAT TGTGCGTGAC CTATCTCCCT AAGTTTGCCA TTCCAAGTGA GCATCGATAC 
GCTAAGCGTA GTCAACGTGA TACTATCCCA GTCGTTGAAG TGGAAATTTG TGGGATACAT 
CTTATATACT CCAAAAATGA TCACCAACAG AATCAGAGCC ATTGGCGTGA AGCCATGATA 
AAAGATCAAG ACATCTCCTG TGATTTCCAA GCTTCTCCCT ACAGGTCCTT TCGGATGAAA 
GAAATCCCCA AGTGGATCAA CGCGTGGAGG TCTAGTGAAA GCGACAGCTC TAATACAGAA 
GGCTCAGAAT CAGAGAACTC TGATTACTAT TCTGCTGACG AGGGGGACCC CACTGTTGAC 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010644027.1
CDS	230..3955
Translation	MASTSVQMASDYSSSTPRCTYDVFLSFRGEDTRNNFTDHLYTALVQRGINTFRDDKLRRGEEIAPELLKAIEESRSSIVVFSKTYAHSRWCLDELAKIMECRREYRQIVLPIFYHVDPADVRKQTGSFGEAFTSYEENWKNKAQRWREALTEAGYIAGWPINKGYESRPIEEIINHILKRLNPKFLPIKEHMVGMYVHLEELKSLLKMQLDDVRMVGIYGIGGIGKTTIAKMVYNDILCQFNGASFLEGVKNRSKCYNDQLQLLQELLHGIMEGGHLKLESINDGMNMIKGRLGSKKVLVVFDDVDDLDQVRGIVANYKWFGGGSRIIITTRDKHLLDQYEVHASYEAKVLCYEDAIELFSWHAFKVQNIREDYVEMSNSMIKYAQGLPLALEVLGSSLYNKTKDEWKSAIEKLKKNPNKKINDVLKISLDGLDRTQREIFLHIACFFKGEAKDFILRILDDHAEYDIGVLCDRCLITISYNKVEMHDLIQQMGWTIDREKHLKDPSKWIRLWDPDDISKAFSAQEGMEQVEVISYDLSRSKEMQVNKKVYENMKELRFLKLHWGDHHGFTTKKYKVFLPKDFEFPSQKLRYLYWDGYPLQTLPSKFNGENLVELHMKNSTIKQLWNGRKILGNLKIIDLSRSRLLTKMPELSSMPNLEELNLVCCERLKKFPEIRENMGRLERVHLDCSGIQEIPSSIEYLPALEFLTLHYCRNFDKFPDNFGNLRHLRVINANRTDIKELPEIHNMGSLTKLFLIETAIKELPRSIGHLTELEELNLENCKNLRSLPNSICGLKSLGVLNLNGCSNLVAFPEIMEDMEDLRELLLSKTPITELPPSIEHLKGLEHLELKNCENLVTLPDSIGNLTHLRSLCVRNCSKLHNLPDNLRSLQWCLRRLDLAGCNLMKGAIPSDLWCLSLLRFLDVSEIPIPCIPTNIIQLSNLRTLRMNHCQMLEEIPELPSRLEILEAQGCPHLGTLSTPSSPLWSYLLNLFKSRTQSCEYEIDSDSLWYFHVPKVVIPGSGGIPKWISHPSMGRQAIIELPKNRYEDNNFLGFAVFFHHVPLDDFWSHWHRRFLQFELRISHDDQSERVIKIMSCFHFETCRSSSSSSEGICLDNVSTSDPPLCVTYLPKFAIPSEHRYAKRSQRDTIPVVEVEICGIHLIYSKNDHQQNQSHWREAMIKDQDISCDFQASPYRSFRMKEIPKWINAWRSSESDSSNTEGSESENSDYYSADEGDPTVDC

Target ORF information:

RefSeq Version	XM_010645725.2
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010645725.2

ORF Insert Sequence:

ATGGCTTCCA CAAGCGTTCA AATGGCGTCT GATTATTCTT CTTCCACCCC TAGATGCACG 
TATGACGTGT TCTTGAGTTT TAGAGGGGAA GACACCCGCA ACAATTTCAC CGATCACCTG 
TATACTGCTT TGGTTCAAAG GGGCATCAAT ACTTTTAGAG ATGATAAACT TAGAAGAGGA 
GAAGAGATTG CACCAGAGCT TCTAAAAGCT ATTGAAGAAT CAAGATCTTC CATTGTTGTT 
TTCTCAAAAA CTTATGCTCA TTCAAGGTGG TGTTTAGACG AGCTTGCAAA GATCATGGAG 
TGTAGACGTG AATATAGGCA AATAGTATTG CCAATTTTCT ACCATGTGGA TCCAGCGGAT 
GTGCGTAAAC AAACAGGGAG TTTTGGAGAA GCATTCACCA GCTATGAAGA AAATTGGAAG 
AACAAGGCAC AAAGGTGGAG GGAGGCTTTG ACCGAAGCAG GCTATATTGC TGGATGGCCT 
ATAAACAAGG GGTACGAGTC AAGGCCTATT GAGGAAATTA TTAACCACAT CCTCAAAAGA 
TTGAATCCTA AGTTTTTGCC TATTAAGGAG CACATGGTTG GAATGTATGT TCACTTGGAA 
GAACTAAAAT CATTGTTAAA AATGCAGTTG GATGATGTTC GCATGGTTGG GATCTATGGA 
ATTGGTGGAA TTGGCAAAAC TACTATTGCC AAGATGGTTT ATAATGACAT CCTATGTCAA 
TTCAATGGTG CTAGCTTTCT TGAAGGTGTC AAAAATAGAT CCAAATGTTA CAATGATCAA 
CTTCAATTAC TGCAAGAACT TCTTCATGGC ATTATGGAGG GTGGACATTT AAAATTAGAA 
AGTATTAATG ATGGAATGAA TATGATCAAG GGCAGACTTG GCTCAAAAAA GGTTCTTGTT 
GTTTTTGATG ATGTCGATGA TTTGGACCAA GTACGAGGAA TAGTTGCAAA CTATAAATGG 
TTTGGTGGAG GAAGCAGAAT CATCATAACT ACCAGAGACA AACATTTGCT AGATCAATAT 
GAGGTGCATG CATCATATGA GGCTAAGGTG TTATGTTATG AAGATGCTAT TGAACTCTTT 
AGTTGGCATG CCTTCAAAGT ACAAAACATA CGTGAAGATT ATGTAGAGAT GTCAAATAGT 
ATGATAAAAT ATGCGCAAGG CCTTCCTTTA GCCCTTGAAG TTTTGGGCTC TTCTCTTTAT 
AACAAGACAA AAGATGAATG GAAAAGTGCA ATAGAGAAAT TGAAAAAAAA CCCTAACAAG 
AAAATTAATG ATGTGCTTAA AATAAGCCTT GATGGGCTTG ATCGTACTCA AAGGGAGATT 
TTCCTTCATA TCGCATGCTT TTTCAAGGGA GAAGCTAAAG ATTTTATATT GAGAATATTA 
GATGATCATG CAGAATATGA CATAGGAGTT CTTTGTGATA GATGCCTAAT AACTATTTCC 
TACAACAAGG TAGAAATGCA TGACTTGATA CAACAAATGG GTTGGACAAT TGATCGTGAA 
AAACATCTTA AAGACCCAAG CAAATGGATC AGATTGTGGG ATCCAGATGA TATTTCTAAA 
GCATTTTCTG CACAAGAGGG AATGGAACAA GTTGAAGTCA TATCATATGA CTTGTCTCGA 
TCAAAAGAAA TGCAGGTGAA TAAAAAGGTT TATGAAAATA TGAAGGAACT AAGATTTCTC 
AAACTCCATT GGGGGGATCA TCATGGTTTT ACGACGAAGA AATATAAAGT GTTCCTTCCC 
AAAGACTTTG AGTTTCCTTC TCAAAAGTTA AGATATCTTT ATTGGGATGG ATACCCTTTG 
CAAACCTTGC CATCAAAGTT TAATGGAGAG AATCTTGTTG AGCTCCACAT GAAAAATAGC 
ACCATAAAAC AACTTTGGAA TGGGCGTAAG ATTCTAGGAA ATTTAAAGAT CATTGACTTA 
AGTAGATCAC GATTGCTCAC CAAAATGCCA GAATTATCAA GCATGCCAAA TCTTGAGGAG 
CTTAACCTCG TGTGTTGTGA AAGGTTGAAG AAATTTCCTG AGATCCGTGA AAATATGGGA 
CGTTTGGAGA GAGTTCATTT AGATTGCAGT GGAATTCAGG AAATACCGAG CAGTATTGAG 
TACTTGCCAG CTCTTGAATT TCTTACACTT CATTATTGCA GAAATTTTGA CAAATTTCCG 
GATAATTTTG GGAATTTGAG ACATCTAAGG GTCATCAATG CAAACAGGAC TGATATTAAA 
GAACTTCCTG AGATCCATAA TATGGGAAGT CTAACGAAAC TTTTTCTAAT CGAGACTGCT 
ATTAAAGAAT TACCACGCTC AATAGGTCAT CTCACCGAAC TTGAGGAGTT AAATCTAGAA 
AATTGCAAAA ATTTGAGGAG TCTTCCTAAT AGCATTTGTG GGTTGAAATC ACTTGGAGTC 
CTCAATCTCA ATGGCTGTTC AAATCTGGTG GCTTTTCCAG AAATCATGGA GGATATGGAA 
GACTTAAGAG AACTTCTTTT AAGTAAAACG CCTATAACAG AGCTGCCACC ATCAATTGAA 
CATCTAAAAG GTCTTGAACA CTTGGAATTG AAGAATTGTG AGAACCTTGT GACTCTTCCA 
GATAGCATTG GTAATTTGAC ACATCTTCGT AGTCTTTGTG TTCGTAACTG TTCGAAGCTC 
CACAATTTGC CCGACAATCT GAGAAGCTTG CAATGGTGCT TAAGAAGACT GGATCTAGCT 
GGTTGCAATC TGATGAAAGG AGCAATTCCT AGTGATTTAT GGTGCCTATC CTTACTGAGA 
TTTTTAGATG TGAGCGAAAT CCCTATTCCT TGCATACCTA CTAACATAAT CCAACTCTCT 
AACCTGAGAA CGCTTAGGAT GAATCACTGC CAGATGCTTG AAGAAATTCC AGAGCTTCCA 
TCAAGGCTAG AAATCTTAGA AGCACAAGGT TGTCCACATC TGGGAACTTT ATCAACTCCT 
TCGAGCCCAC TCTGGTCTTA TCTCCTCAAC CTCTTCAAAT CACGTACTCA GAGTTGTGAA 
TACGAAATAG ACTCAGACTC CTTATGGTAC TTCCATGTAC CTAAAGTTGT TATTCCAGGA 
AGTGGTGGAA TACCAAAGTG GATAAGTCAT CCGAGTATGG GACGCCAAGC AATAATAGAG 
CTCCCAAAGA ATCGGTATGA AGATAACAAT TTCTTGGGAT TTGCTGTTTT CTTCCATCAT 
GTTCCACTTG ATGATTTTTG GTCGCATTGG CATAGGCGTT TTCTACAATT TGAATTGAGG 
ATATCCCATG ATGATCAATC TGAACGGGTA ATTAAAATAA TGAGTTGTTT CCATTTTGAA 
ACATGCCGTA GTAGTAGTTC ATCATCTGAG GGTATATGCC TCGACAATGT TAGCACATCG 
GATCCACCAT TGTGCGTGAC CTATCTCCCT AAGTTTGCCA TTCCAAGTGA GCATCGATAC 
GCTAAGCGTA GTCAACGTGA TACTATCCCA GTCGTTGAAG TGGAAATTTG TGGGATACAT 
CTTATATACT CCAAAAATGA TCACCAACAG AATCAGAGCC ATTGGCGTGA AGCCATGATA 
AAAGATCAAG ACATCTCCTG TGATTTCCAA GCTTCTCCCT ACAGGTCCTT TCGGATGAAA 
GAAATCCCCA AGTGGATCAA CGCGTGGAGG TCTAGTGAAA GCGACAGCTC TAATACAGAA 
GGCTCAGAAT CAGAGAACTC TGATTACTAT TCTGCTGACG AGGGGGACCC CACTGTTGAC 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100241404 ( XM_019216195.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019071740.1 Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X3, mRNA.

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CloneID	OVa43230
Clone ID Related Accession (Same CDS sequence)	XM_019216195.1
Accession Version	XM_019216195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	disease resistance protein TAO1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012024.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAGG ATCATGAGTA CGAGTCAAGG CCTATTGAGG AAATTATTAA CCACATCCTC 
AAAAGATTGA ATCCTAAGTT TTTGCCTATT AAGGAGCACA TGGTTGGAAT GTATGTTCAC 
TTGGAAGAAC TAAAATCATT GTTAAAAATG CAGTTGGATG ATGTTCGCAT GGTTGGGATC 
TATGGAATTG GTGGAATTGG CAAAACTACT ATTGCCAAGA TGGTTTATAA TGACATCCTA 
TGTCAATTCA ATGGTGCTAG CTTTCTTGAA GGTGTCAAAA ATAGATCCAA ATGTTACAAT 
GATCAACTTC AATTACTGCA AGAACTTCTT CATGGCATTA TGGAGGGTGG ACATTTAAAA 
TTAGAAAGTA TTAATGATGG AATGAATATG ATCAAGGGCA GACTTGGCTC AAAAAAGGTT 
CTTGTTGTTT TTGATGATGT CGATGATTTG GACCAAGTAC GAGGAATAGT TGCAAACTAT 
AAATGGTTTG GTGGAGGAAG CAGAATCATC ATAACTACCA GAGACAAACA TTTGCTAGAT 
CAATATGAGG TGCATGCATC ATATGAGGCT AAGGTGTTAT GTTATGAAGA TGCTATTGAA 
CTCTTTAGTT GGCATGCCTT CAAAGTACAA AACATACGTG AAGATTATGT AGAGATGTCA 
AATAGTATGA TAAAATATGC GCAAGGCCTT CCTTTAGCCC TTGAAGTTTT GGGCTCTTCT 
CTTTATAACA AGACAAAAGA TGAATGGAAA AGTGCAATAG AGAAATTGAA AAAAAACCCT 
AACAAGAAAA TTAATGATGT GCTTAAAATA AGCCTTGATG GGCTTGATCG TACTCAAAGG 
GAGATTTTCC TTCATATCGC ATGCTTTTTC AAGGGAGAAG CTAAAGATTT TATATTGAGA 
ATATTAGATG ATCATGCAGA ATATGACATA GGAGTTCTTT GTGATAGATG CCTAATAACT 
ATTTCCTACA ACAAGGTAGA AATGCATGAC TTGATACAAC AAATGGGTTG GACAATTGAT 
CGTGAAAAAC ATCTTAAAGA CCCAAGCAAA TGGATCAGAT TGTGGGATCC AGATGATATT 
TCTAAAGCAT TTTCTGCACA AGAGGGAATG GAACAAGTTG AAGTCATATC ATATGACTTG 
TCTCGATCAA AAGAAATGCA GGTGAATAAA AAGGTTTATG AAAATATGAA GGAACTAAGA 
TTTCTCAAAC TCCATTGGGG GGATCATCAT GGTTTTACGA CGAAGAAATA TAAAGTGTTC 
CTTCCCAAAG ACTTTGAGTT TCCTTCTCAA AAGTTAAGAT ATCTTTATTG GGATGGATAC 
CCTTTGCAAA CCTTGCCATC AAAGTTTAAT GGAGAGAATC TTGTTGAGCT CCACATGAAA 
AATAGCACCA TAAAACAACT TTGGAATGGG CGTAAGATTC TAGGAAATTT AAAGATCATT 
GACTTAAGTA GATCACGATT GCTCACCAAA ATGCCAGAAT TATCAAGCAT GCCAAATCTT 
GAGGAGCTTA ACCTCGTGTG TTGTGAAAGG TTGAAGAAAT TTCCTGAGAT CCGTGAAAAT 
ATGGGACGTT TGGAGAGAGT TCATTTAGAT TGCAGTGGAA TTCAGGAAAT ACCGAGCAGT 
ATTGAGTACT TGCCAGCTCT TGAATTTCTT ACACTTCATT ATTGCAGAAA TTTTGACAAA 
TTTCCGGATA ATTTTGGGAA TTTGAGACAT CTAAGGGTCA TCAATGCAAA CAGGACTGAT 
ATTAAAGAAC TTCCTGAGAT CCATAATATG GGAAGTCTAA CGAAACTTTT TCTAATCGAG 
ACTGCTATTA AAGAATTACC ACGCTCAATA GGTCATCTCA CCGAACTTGA GGAGTTAAAT 
CTAGAAAATT GCAAAAATTT GAGGAGTCTT CCTAATAGCA TTTGTGGGTT GAAATCACTT 
GGAGTCCTCA ATCTCAATGG CTGTTCAAAT CTGGTGGCTT TTCCAGAAAT CATGGAGGAT 
ATGGAAGACT TAAGAGAACT TCTTTTAAGT AAAACGCCTA TAACAGAGCT GCCACCATCA 
ATTGAACATC TAAAAGGTCT TGAACACTTG GAATTGAAGA ATTGTGAGAA CCTTGTGACT 
CTTCCAGATA GCATTGGTAA TTTGACACAT CTTCGTAGTC TTTGTGTTCG TAACTGTTCG 
AAGCTCCACA ATTTGCCCGA CAATCTGAGA AGCTTGCAAT GGTGCTTAAG AAGACTGGAT 
CTAGCTGGTT GCAATCTGAT GAAAGGAGCA ATTCCTAGTG ATTTATGGTG CCTATCCTTA 
CTGAGATTTT TAGATGTGAG CGAAATCCCT ATTCCTTGCA TACCTACTAA CATAATCCAA 
CTCTCTAACC TGAGAACGCT TAGGATGAAT CACTGCCAGA TGCTTGAAGA AATTCCAGAG 
CTTCCATCAA GGCTAGAAAT CTTAGAAGCA CAAGGTTGTC CACATCTGGG AACTTTATCA 
ACTCCTTCGA GCCCACTCTG GTCTTATCTC CTCAACCTCT TCAAATCACG TACTCAGAGT 
TGTGAATACG AAATAGACTC AGACTCCTTA TGGTACTTCC ATGTACCTAA AGTTGTTATT 
CCAGGAAGTG GTGGAATACC AAAGTGGATA AGTCATCCGA GTATGGGACG CCAAGCAATA 
ATAGAGCTCC CAAAGAATCG GTATGAAGAT AACAATTTCT TGGGATTTGC TGTTTTCTTC 
CATCATGTTC CACTTGATGA TTTTTGGTCG CATTGGCATA GGCGTTTTCT ACAATTTGAA 
TTGAGGATAT CCCATGATGA TCAATCTGAA CGGGTAATTA AAATAATGAG TTGTTTCCAT 
TTTGAAACAT GCCGTAGTAG TAGTTCATCA TCTGAGGGTA TATGCCTCGA CAATGTTAGC 
ACATCGGATC CACCATTGTG CGTGACCTAT CTCCCTAAGT TTGCCATTCC AAGTGAGCAT 
CGATACGCTA AGCGTAGTCA ACGTGATACT ATCCCAGTCG TTGAAGTGGA AATTTGTGGG 
ATACATCTTA TATACTCCAA AAATGATCAC CAACAGAATC AGAGCCATTG GCGTGAAGCC 
ATGATAAAAG ATCAAGACAT CTCCTGTGAT TTCCAAGCTT CTCCCTACAG GTCCTTTCGG 
ATGAAAGAAA TCCCCAAGTG GATCAACGCG TGGAGGTCTA GTGAAAGCGA CAGCTCTAAT 
ACAGAAGGCT CAGAATCAGA GAACTCTGAT TACTATTCTG CTGACGAGGG GGACCCCACT 
GTTGACTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019071740.1
CDS	785..4036
Translation	MIKDHEYESRPIEEIINHILKRLNPKFLPIKEHMVGMYVHLEELKSLLKMQLDDVRMVGIYGIGGIGKTTIAKMVYNDILCQFNGASFLEGVKNRSKCYNDQLQLLQELLHGIMEGGHLKLESINDGMNMIKGRLGSKKVLVVFDDVDDLDQVRGIVANYKWFGGGSRIIITTRDKHLLDQYEVHASYEAKVLCYEDAIELFSWHAFKVQNIREDYVEMSNSMIKYAQGLPLALEVLGSSLYNKTKDEWKSAIEKLKKNPNKKINDVLKISLDGLDRTQREIFLHIACFFKGEAKDFILRILDDHAEYDIGVLCDRCLITISYNKVEMHDLIQQMGWTIDREKHLKDPSKWIRLWDPDDISKAFSAQEGMEQVEVISYDLSRSKEMQVNKKVYENMKELRFLKLHWGDHHGFTTKKYKVFLPKDFEFPSQKLRYLYWDGYPLQTLPSKFNGENLVELHMKNSTIKQLWNGRKILGNLKIIDLSRSRLLTKMPELSSMPNLEELNLVCCERLKKFPEIRENMGRLERVHLDCSGIQEIPSSIEYLPALEFLTLHYCRNFDKFPDNFGNLRHLRVINANRTDIKELPEIHNMGSLTKLFLIETAIKELPRSIGHLTELEELNLENCKNLRSLPNSICGLKSLGVLNLNGCSNLVAFPEIMEDMEDLRELLLSKTPITELPPSIEHLKGLEHLELKNCENLVTLPDSIGNLTHLRSLCVRNCSKLHNLPDNLRSLQWCLRRLDLAGCNLMKGAIPSDLWCLSLLRFLDVSEIPIPCIPTNIIQLSNLRTLRMNHCQMLEEIPELPSRLEILEAQGCPHLGTLSTPSSPLWSYLLNLFKSRTQSCEYEIDSDSLWYFHVPKVVIPGSGGIPKWISHPSMGRQAIIELPKNRYEDNNFLGFAVFFHHVPLDDFWSHWHRRFLQFELRISHDDQSERVIKIMSCFHFETCRSSSSSSEGICLDNVSTSDPPLCVTYLPKFAIPSEHRYAKRSQRDTIPVVEVEICGIHLIYSKNDHQQNQSHWREAMIKDQDISCDFQASPYRSFRMKEIPKWINAWRSSESDSSNTEGSESENSDYYSADEGDPTVDC

Target ORF information:

RefSeq Version	XM_019216195.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera TMV resistance protein N (LOC100241404), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019216195.1

ORF Insert Sequence:

ATGATCAAGG ATCATGAGTA CGAGTCAAGG CCTATTGAGG AAATTATTAA CCACATCCTC 
AAAAGATTGA ATCCTAAGTT TTTGCCTATT AAGGAGCACA TGGTTGGAAT GTATGTTCAC 
TTGGAAGAAC TAAAATCATT GTTAAAAATG CAGTTGGATG ATGTTCGCAT GGTTGGGATC 
TATGGAATTG GTGGAATTGG CAAAACTACT ATTGCCAAGA TGGTTTATAA TGACATCCTA 
TGTCAATTCA ATGGTGCTAG CTTTCTTGAA GGTGTCAAAA ATAGATCCAA ATGTTACAAT 
GATCAACTTC AATTACTGCA AGAACTTCTT CATGGCATTA TGGAGGGTGG ACATTTAAAA 
TTAGAAAGTA TTAATGATGG AATGAATATG ATCAAGGGCA GACTTGGCTC AAAAAAGGTT 
CTTGTTGTTT TTGATGATGT CGATGATTTG GACCAAGTAC GAGGAATAGT TGCAAACTAT 
AAATGGTTTG GTGGAGGAAG CAGAATCATC ATAACTACCA GAGACAAACA TTTGCTAGAT 
CAATATGAGG TGCATGCATC ATATGAGGCT AAGGTGTTAT GTTATGAAGA TGCTATTGAA 
CTCTTTAGTT GGCATGCCTT CAAAGTACAA AACATACGTG AAGATTATGT AGAGATGTCA 
AATAGTATGA TAAAATATGC GCAAGGCCTT CCTTTAGCCC TTGAAGTTTT GGGCTCTTCT 
CTTTATAACA AGACAAAAGA TGAATGGAAA AGTGCAATAG AGAAATTGAA AAAAAACCCT 
AACAAGAAAA TTAATGATGT GCTTAAAATA AGCCTTGATG GGCTTGATCG TACTCAAAGG 
GAGATTTTCC TTCATATCGC ATGCTTTTTC AAGGGAGAAG CTAAAGATTT TATATTGAGA 
ATATTAGATG ATCATGCAGA ATATGACATA GGAGTTCTTT GTGATAGATG CCTAATAACT 
ATTTCCTACA ACAAGGTAGA AATGCATGAC TTGATACAAC AAATGGGTTG GACAATTGAT 
CGTGAAAAAC ATCTTAAAGA CCCAAGCAAA TGGATCAGAT TGTGGGATCC AGATGATATT 
TCTAAAGCAT TTTCTGCACA AGAGGGAATG GAACAAGTTG AAGTCATATC ATATGACTTG 
TCTCGATCAA AAGAAATGCA GGTGAATAAA AAGGTTTATG AAAATATGAA GGAACTAAGA 
TTTCTCAAAC TCCATTGGGG GGATCATCAT GGTTTTACGA CGAAGAAATA TAAAGTGTTC 
CTTCCCAAAG ACTTTGAGTT TCCTTCTCAA AAGTTAAGAT ATCTTTATTG GGATGGATAC 
CCTTTGCAAA CCTTGCCATC AAAGTTTAAT GGAGAGAATC TTGTTGAGCT CCACATGAAA 
AATAGCACCA TAAAACAACT TTGGAATGGG CGTAAGATTC TAGGAAATTT AAAGATCATT 
GACTTAAGTA GATCACGATT GCTCACCAAA ATGCCAGAAT TATCAAGCAT GCCAAATCTT 
GAGGAGCTTA ACCTCGTGTG TTGTGAAAGG TTGAAGAAAT TTCCTGAGAT CCGTGAAAAT 
ATGGGACGTT TGGAGAGAGT TCATTTAGAT TGCAGTGGAA TTCAGGAAAT ACCGAGCAGT 
ATTGAGTACT TGCCAGCTCT TGAATTTCTT ACACTTCATT ATTGCAGAAA TTTTGACAAA 
TTTCCGGATA ATTTTGGGAA TTTGAGACAT CTAAGGGTCA TCAATGCAAA CAGGACTGAT 
ATTAAAGAAC TTCCTGAGAT CCATAATATG GGAAGTCTAA CGAAACTTTT TCTAATCGAG 
ACTGCTATTA AAGAATTACC ACGCTCAATA GGTCATCTCA CCGAACTTGA GGAGTTAAAT 
CTAGAAAATT GCAAAAATTT GAGGAGTCTT CCTAATAGCA TTTGTGGGTT GAAATCACTT 
GGAGTCCTCA ATCTCAATGG CTGTTCAAAT CTGGTGGCTT TTCCAGAAAT CATGGAGGAT 
ATGGAAGACT TAAGAGAACT TCTTTTAAGT AAAACGCCTA TAACAGAGCT GCCACCATCA 
ATTGAACATC TAAAAGGTCT TGAACACTTG GAATTGAAGA ATTGTGAGAA CCTTGTGACT 
CTTCCAGATA GCATTGGTAA TTTGACACAT CTTCGTAGTC TTTGTGTTCG TAACTGTTCG 
AAGCTCCACA ATTTGCCCGA CAATCTGAGA AGCTTGCAAT GGTGCTTAAG AAGACTGGAT 
CTAGCTGGTT GCAATCTGAT GAAAGGAGCA ATTCCTAGTG ATTTATGGTG CCTATCCTTA 
CTGAGATTTT TAGATGTGAG CGAAATCCCT ATTCCTTGCA TACCTACTAA CATAATCCAA 
CTCTCTAACC TGAGAACGCT TAGGATGAAT CACTGCCAGA TGCTTGAAGA AATTCCAGAG 
CTTCCATCAA GGCTAGAAAT CTTAGAAGCA CAAGGTTGTC CACATCTGGG AACTTTATCA 
ACTCCTTCGA GCCCACTCTG GTCTTATCTC CTCAACCTCT TCAAATCACG TACTCAGAGT 
TGTGAATACG AAATAGACTC AGACTCCTTA TGGTACTTCC ATGTACCTAA AGTTGTTATT 
CCAGGAAGTG GTGGAATACC AAAGTGGATA AGTCATCCGA GTATGGGACG CCAAGCAATA 
ATAGAGCTCC CAAAGAATCG GTATGAAGAT AACAATTTCT TGGGATTTGC TGTTTTCTTC 
CATCATGTTC CACTTGATGA TTTTTGGTCG CATTGGCATA GGCGTTTTCT ACAATTTGAA 
TTGAGGATAT CCCATGATGA TCAATCTGAA CGGGTAATTA AAATAATGAG TTGTTTCCAT 
TTTGAAACAT GCCGTAGTAG TAGTTCATCA TCTGAGGGTA TATGCCTCGA CAATGTTAGC 
ACATCGGATC CACCATTGTG CGTGACCTAT CTCCCTAAGT TTGCCATTCC AAGTGAGCAT 
CGATACGCTA AGCGTAGTCA ACGTGATACT ATCCCAGTCG TTGAAGTGGA AATTTGTGGG 
ATACATCTTA TATACTCCAA AAATGATCAC CAACAGAATC AGAGCCATTG GCGTGAAGCC 
ATGATAAAAG ATCAAGACAT CTCCTGTGAT TTCCAAGCTT CTCCCTACAG GTCCTTTCGG 
ATGAAAGAAA TCCCCAAGTG GATCAACGCG TGGAGGTCTA GTGAAAGCGA CAGCTCTAAT 
ACAGAAGGCT CAGAATCAGA GAACTCTGAT TACTATTCTG CTGACGAGGG GGACCCCACT 
GTTGACTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100241404 cDNA ORF clone, Vitis vinifera(wine grape)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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