ORF » Species Summary » Thalassiosira pseudonana CCMP1335 » LEU2 cDNA ORF clone

LEU2 cDNA ORF clone, Thalassiosira pseudonana CCMP1335

The following LEU2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEU2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OTf05492 XM_002294386.1
Latest version!		 Thalassiosira pseudonana CCMP1335 3-isopropylmalate dehydratase (LEU2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LEU2 ( XM_002294386.1 ) cDNA ORF clone, Thalassiosira pseudonana CCMP1335 -> XP_002294422.1 Thalassiosira pseudonana CCMP1335 3-isopropylmalate dehydratase (LEU2), mRNA.
    Price & Availability↑
    CloneID OTf05492
    Clone ID Related Accession (Same CDS sequence) XM_002294386.1
    Accession Version XM_002294386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Thalassiosira pseudonana CCMP1335
    Product 3-isopropylmalate dehydratase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012080). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATATTTC GCTTCGCTGC TGTGGCAGCC ACCCTCGTTC TCTCCCGAAG CGTCGAGTCC 
    TTCGCCCCGC ATAAATCCTT CACGCGTCCC ATCGTCAGCG TCGGTGCATC CACCACCTCC 
    CTCTCCGCCG CATCCACCCT CTACGACAAA ATCTGGTCCG ACCACGTAGT CAACGACGAC 
    GGCCTCTCCT CCCTCCTCTA CATCGACCGC CACCTAGTCC ACGAGGTGAC CTCCCCCCAA 
    GCATTCGAGG GCCTCCGTCT CGCCAACCGC TCCGTCCGTC GACCCGACTG CACCCTGTCC 
    ACGGTCGATC ACAACGTTCC CACCACCGAC CGATCGGCCT TGGTGGATGT GGCGAGTTTC 
    ATCGAGGAGA CGGCGAGTCG TACGCAGGTG TTGCAGTTGC AGACGAATGT GGCGGATTTT 
    GATATTCCGT ATTTTGGAAT GGAGGACGAG AGGCAGGGGA TTGTGCATAT CATTGGACCG 
    GAGCAGGGGT TTACGTTGCC GGGGACTACC TGTGTTTGTG GGGATAGTCA TACGGCGACT 
    CATGGGGCGT TTGGGGCGTT GGCGTTTGGG ATTGGGACGA GCGAGGTGGA GCATGTGTTG 
    GCTACTCAGA CCCTCCCCCA AACCAAGGCC AAAAACATGC TCATCCGCAT CGACGGCGAT 
    CTTCTCCCCG GCGTCACCTC CAAAGACATT ATCCTCCACA TCTGCTCCGT CATCGGCACC 
    GCCGGTGGAA CGGGTGCCAC CATTGAATTC GCCGGAAAGG CTATTGAGAA CCTATCCATG 
    GAGGCTAGGA TGAGTATCTC CAATATGGCC ATTGAGGCTG GTGCTCGTGC TGGAATCATT 
    GCTCCGGATG ATGTTACTTT TGAATACCTT AAGGGGAGGC CAATGTGTCC TTCGGGAGAA 
    GATTGGGATA GAGCCGTGGA GTATTGGAGA TCGTTGAAAA CGGATGAGGG AGCTGTGTTT 
    GATAAGACTG TGGTCATTGA GGCAAAGGAT ATTGCACCTA CTGTTACGTG GGGAACGTCA 
    CCTCAGGATG CCATGCCCGT GGATGGTATC GTTCCTCGTA TTGACGAACC TGGACATGAT 
    GAGGCTCGTC AGGCGGCTGT CAAGCGTAGT TTGGAGTACA TTGGATTGGA GGGAGGGGAA 
    CCCATTGAAG GAACTCCCAT TGGAAAGGTC TTTATCGGAA GTTGCACCAA TGGACGTATC 
    GAGGACATTC GTGAGGTTGC CGCCATTGCG ATTGGACGCA AGGTTGCCGA TGGAGTGCAT 
    GCCATGGTTG TTCCTGGATC TGGACTTGTC AAGCACCAAG CAGAGCAGGA GGGACTCGAC 
    AAAATCTTGA CAGAGGCGGG GTTTGATTGG CGTGAGCCCG GGTGTTCCAT GTGCCTTGCA 
    ATGAACCCTG ATAAGTTGAA GCCACAGGAA CGTTGTGCTT CTACTTCCAA CCGTAACTTT 
    GAGGGAAGGC AAGGAAACGG AGGACGTACC CATTTGATGT CTCCTGCCAT GGCGGCGGCT 
    GCTGCGGTGA GTGGAGGGAT TGCCGATGTG AGGAAGTTTC CATATTTGGG CGAAGAGAGC 
    AATGATCCAC GTGTGGCTAA CAAGGATGTC AGGAGTAATG TGTTCACGAC GGAGTCGTTT 
    GTGTCACCTG GAGCAGTCAT TTCACCACCA GCTCCGTATC AGGCTAGGCA GGGAGAGAGT 
    GCTTCCACTT CCGGTGGAGC TGCTGGGCTT CCCAAGTTTG ACGTGCTCAA GGGAGTTGTC 
    GCTCCTCTCG ATATTCAAAA CATTGATACC GATATGATCA TCCCCAAGGA ATTCCTCAAG 
    ACTATTCAAC GTGCTGGATT GGGATTTGCT GCTTTTGCTG AATTGAGGTA TGAAAATCCT 
    GAGGAGGTTG CCACTATTGG AGCGGATGTT GCCAAGCCTC GTTCGGACTT TGTCTTGAAT 
    CAGCCTGGGT ATGAAAAGAC TTCCATCCTT ATTGCGGGAG ATAACTTTGG ATGTGGAAGT 
    AGTAGGGAGC ATGCTCCTTG GTCTATCAAT GACATGGGTA TTCGGTGTAT CATCAGCACG 
    TCGTTTGCGG ATATCTTTTA CAACAACTGC ATCAACAATG GAATGTTGCC GGTCACTTTG 
    GATAGGGCGA GTGTGGAGAA GCTTTTGGAG GACGCCAAGG TTGCTGGAAC GGAGATTACG 
    GTGGATCTCG TGAATAGCAA GGTCATTCGT CCCAACGGAG AGGAATTCCC ATTTGAAATC 
    GATGCTTTCA AGAAGCACTG TCTCTTGAAC GGTCTTGATA AGATTGGACT CACCCTTGAT 
    AAGGAGGAAA TGATTATTGC CTTTGAGAAG GAACGATCTG AGAAATATCC TTGGTTGGAC 
    GGTGCTTCAA TGAAAGTCCC TCAGAAGATT GCCATGTATC CTAGTGCTGG AATTTGGAGC 
    AAGGTGGAAG CGTCTGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002294422.1
    CDS41..2461
    Translation

    Target ORF information:

    RefSeq Version XM_002294386.1
    Organism Thalassiosira pseudonana CCMP1335
    Definition Thalassiosira pseudonana CCMP1335 3-isopropylmalate dehydratase (LEU2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_002294386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATATTTC GCTTCGCTGC TGTGGCAGCC ACCCTCGTTC TCTCCCGAAG CGTCGAGTCC 
    TTCGCCCCGC ATAAATCCTT CACGCGTCCC ATCGTCAGCG TCGGTGCATC CACCACCTCC 
    CTCTCCGCCG CATCCACCCT CTACGACAAA ATCTGGTCCG ACCACGTAGT CAACGACGAC 
    GGCCTCTCCT CCCTCCTCTA CATCGACCGC CACCTAGTCC ACGAGGTGAC CTCCCCCCAA 
    GCATTCGAGG GCCTCCGTCT CGCCAACCGC TCCGTCCGTC GACCCGACTG CACCCTGTCC 
    ACGGTCGATC ACAACGTTCC CACCACCGAC CGATCGGCCT TGGTGGATGT GGCGAGTTTC 
    ATCGAGGAGA CGGCGAGTCG TACGCAGGTG TTGCAGTTGC AGACGAATGT GGCGGATTTT 
    GATATTCCGT ATTTTGGAAT GGAGGACGAG AGGCAGGGGA TTGTGCATAT CATTGGACCG 
    GAGCAGGGGT TTACGTTGCC GGGGACTACC TGTGTTTGTG GGGATAGTCA TACGGCGACT 
    CATGGGGCGT TTGGGGCGTT GGCGTTTGGG ATTGGGACGA GCGAGGTGGA GCATGTGTTG 
    GCTACTCAGA CCCTCCCCCA AACCAAGGCC AAAAACATGC TCATCCGCAT CGACGGCGAT 
    CTTCTCCCCG GCGTCACCTC CAAAGACATT ATCCTCCACA TCTGCTCCGT CATCGGCACC 
    GCCGGTGGAA CGGGTGCCAC CATTGAATTC GCCGGAAAGG CTATTGAGAA CCTATCCATG 
    GAGGCTAGGA TGAGTATCTC CAATATGGCC ATTGAGGCTG GTGCTCGTGC TGGAATCATT 
    GCTCCGGATG ATGTTACTTT TGAATACCTT AAGGGGAGGC CAATGTGTCC TTCGGGAGAA 
    GATTGGGATA GAGCCGTGGA GTATTGGAGA TCGTTGAAAA CGGATGAGGG AGCTGTGTTT 
    GATAAGACTG TGGTCATTGA GGCAAAGGAT ATTGCACCTA CTGTTACGTG GGGAACGTCA 
    CCTCAGGATG CCATGCCCGT GGATGGTATC GTTCCTCGTA TTGACGAACC TGGACATGAT 
    GAGGCTCGTC AGGCGGCTGT CAAGCGTAGT TTGGAGTACA TTGGATTGGA GGGAGGGGAA 
    CCCATTGAAG GAACTCCCAT TGGAAAGGTC TTTATCGGAA GTTGCACCAA TGGACGTATC 
    GAGGACATTC GTGAGGTTGC CGCCATTGCG ATTGGACGCA AGGTTGCCGA TGGAGTGCAT 
    GCCATGGTTG TTCCTGGATC TGGACTTGTC AAGCACCAAG CAGAGCAGGA GGGACTCGAC 
    AAAATCTTGA CAGAGGCGGG GTTTGATTGG CGTGAGCCCG GGTGTTCCAT GTGCCTTGCA 
    ATGAACCCTG ATAAGTTGAA GCCACAGGAA CGTTGTGCTT CTACTTCCAA CCGTAACTTT 
    GAGGGAAGGC AAGGAAACGG AGGACGTACC CATTTGATGT CTCCTGCCAT GGCGGCGGCT 
    GCTGCGGTGA GTGGAGGGAT TGCCGATGTG AGGAAGTTTC CATATTTGGG CGAAGAGAGC 
    AATGATCCAC GTGTGGCTAA CAAGGATGTC AGGAGTAATG TGTTCACGAC GGAGTCGTTT 
    GTGTCACCTG GAGCAGTCAT TTCACCACCA GCTCCGTATC AGGCTAGGCA GGGAGAGAGT 
    GCTTCCACTT CCGGTGGAGC TGCTGGGCTT CCCAAGTTTG ACGTGCTCAA GGGAGTTGTC 
    GCTCCTCTCG ATATTCAAAA CATTGATACC GATATGATCA TCCCCAAGGA ATTCCTCAAG 
    ACTATTCAAC GTGCTGGATT GGGATTTGCT GCTTTTGCTG AATTGAGGTA TGAAAATCCT 
    GAGGAGGTTG CCACTATTGG AGCGGATGTT GCCAAGCCTC GTTCGGACTT TGTCTTGAAT 
    CAGCCTGGGT ATGAAAAGAC TTCCATCCTT ATTGCGGGAG ATAACTTTGG ATGTGGAAGT 
    AGTAGGGAGC ATGCTCCTTG GTCTATCAAT GACATGGGTA TTCGGTGTAT CATCAGCACG 
    TCGTTTGCGG ATATCTTTTA CAACAACTGC ATCAACAATG GAATGTTGCC GGTCACTTTG 
    GATAGGGCGA GTGTGGAGAA GCTTTTGGAG GACGCCAAGG TTGCTGGAAC GGAGATTACG 
    GTGGATCTCG TGAATAGCAA GGTCATTCGT CCCAACGGAG AGGAATTCCC ATTTGAAATC 
    GATGCTTTCA AGAAGCACTG TCTCTTGAAC GGTCTTGATA AGATTGGACT CACCCTTGAT 
    AAGGAGGAAA TGATTATTGC CTTTGAGAAG GAACGATCTG AGAAATATCC TTGGTTGGAC 
    GGTGCTTCAA TGAAAGTCCC TCAGAAGATT GCCATGTATC CTAGTGCTGG AATTTGGAGC 
    AAGGTGGAAG CGTCTGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Phaeodactylum genome reveals the evolutionary history of diatom genomes.
    Nature456(7219)239-44(2008 Nov)
    Bowler C,Allen AE,Badger JH,Grimwood J,Jabbari K,Kuo A,Maheswari U,Martens C,Maumus F,Otillar RP,Rayko E,Salamov A,Vandepoele K,Beszteri B,Gruber A,Heijde M,Katinka M,Mock T,Valentin K,Verret F,Berges JA,Brownlee C,Cadoret JP,Chiovitti A,Choi CJ,Coesel S,De Martino A,Detter JC,Durkin C,Falciatore A,Fournet J,Haruta M,Huysman MJ,Jenkins BD,Jiroutova K,Jorgensen RE,Joubert Y,Kaplan A,Kröger N,Kroth PG,La Roche J,Lindquist E,Lommer M,Martin-Jézéquel V,Lopez PJ,Lucas S,Mangogna M,McGinnis K,Medlin LK,Montsant A,Oudot-Le Secq MP,Napoli C,Obornik M,Parker MS,Petit JL,Porcel BM,Poulsen N,Robison M,Rychlewski L,Rynearson TA,Schmutz J,Shapiro H,Siaut M,Stanley M,Sussman MR,Taylor AR,Vardi A,von Dassow P,Vyverman W,Willis A,Wyrwicz LS,Rokhsar DS,Weissenbach J,Armbrust EV,Green BR,Van de Peer Y,Grigoriev IV


    The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism.
    Science (New York, N.Y.)306(5693)79-86(2004 Oct)
    Armbrust EV,Berges JA,Bowler C,Green BR,Martinez D,Putnam NH,Zhou S,Allen AE,Apt KE,Bechner M,Brzezinski MA,Chaal BK,Chiovitti A,Davis AK,Demarest MS,Detter JC,Glavina T,Goodstein D,Hadi MZ,Hellsten U,Hildebrand M,Jenkins BD,Jurka J,Kapitonov VV,Kröger N,Lau WW,Lane TW,Larimer FW,Lippmeier JC,Lucas S,Medina M,Montsant A,Obornik M,Parker MS,Palenik B,Pazour GJ,Richardson PM,Rynearson TA,Saito MA,Schwartz DC,Thamatrakoln K,Valentin K,Vardi A,Wilkerson FP,Rokhsar DS


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