LOC106606442 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106606442
Entrez Gene ID	106606442
Full Name	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like
Gene Type	protein-coding
Organism	Salmo salar(Atlantic salmon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014202660.1	XP_014058135.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X1
XM_014202673.1	XP_014058148.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X3
XM_014202680.1	XP_014058155.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X4
XM_014202656.1	XP_014058131.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X1
XM_014202667.1	XP_014058142.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X2
XM_014202687.1	XP_014058162.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC106606442 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106606442 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAt05303	XM_014202660.1 Latest version!	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt05303	XM_014202656.1 Latest version!	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt05304	XM_014202667.1 Latest version!	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt05305	XM_014202673.1 Latest version!	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt05306	XM_014202680.1 Latest version!	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt05307	XM_014202687.1 Latest version!	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106606442 ( XM_014202660.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014058135.1 Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAt05303
Clone ID Related Accession (Same CDS sequence)	XM_014202660.1 , XM_014202656.1
Accession Version	XM_014202660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012332512.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGAGGATGG TGCGGCTCTC ATCCCCTACT CCCTGCCCTG TCTGCGGGAG 
CTCTTCCGCT TCCTCATCTC ACTGACCAAC CCCCATGACC GCCACAACAC GGACGCCATG 
ATGCACATGG GCCTGCAGTT GCTGACCGTG GCTCTGGAGG CGGCTCACAT CGCCCCCTAC 
CAGTCACTGC TGGGCCTGGT CAAAGACGAG CTCTGCAGAC ACCTCTTCCA GTTGCTAAGT 
GTGGACCGTA TGAACCTGTA TGCCGCCTCC GTCCGAGCGT GCTTCTTGCT CTTTGAGAGC 
ATGCGAGGAC ATTTGAAGTT TCAGCTCGAG ATGTATCTGA AGAAGCTGAT GGACATCATC 
ACATCAGAGA ACATGAAGAT GCTCTATGAG ATGAAGGAGA TGGCCCTGGA GGCCCTGGTG 
CAGCTCTCGA GGATCCCCAG CTTCGTCACC GAGCTCTACA TCAATTACGA CTGTGACTTC 
TACTGCTCAA ACCTGTTTGA AGACCTCACC AAGCTGCTCT CTAAGAATGC CTTCCCTGTG 
TCCGGACAGC TCTACACAAC CCACCTCCTC TCACTAGAGG CCCTACTGAC TGTTATTGAC 
AGCACTGAGG CCCACTGCCA GGCCAAGGTG CTCAACAGCA GCGCTCAGCA GGACCAATCA 
GCAGAAATGG TGTTGGCAGA AGGGGAGGGC TCAACCAACG ACCGTACAGA CACAACCACG 
GAAGCGAGCA GGCAGGTGAA CAGTGCCAAC GGTCAGAATG TTCCAGAGGC TCACAGTTGG 
CCCCTACCAC CTACCAGCGG GCACCTGATG GCAGAGAAGA TGAGACTGGG CAGACAAGAC 
CAGGAGGAGA CAGAACCAGC TGAGAAGAAA GAACCAAAAA AGCCACAACG CTTCTCGTCT 
TGTTTACCTG ATTCTCAAGA GCTGTTGGAC ATCAGAAATA AAAAGAAGCT GCTGAACAGC 
GGTACAGAGC AGTTCAACCA GAAGCCTAAG AAGGGCATCC AGTTCCTGCA GGAGAAGGGT 
CTCCTCAGCA GCCCCATGGA CAACAACGAG GTGGCCCAGT GGCTCCGGGA GAACCCCCGC 
CTCGACAAGA AGCAGATCGG AGAGTTCATC AGTGACCGCA AATACATGGA GTTACTGGAC 
AGCTTCGTCA ACACGTTCAC CTTCCGAGGG CTGCGTATTG ACGAGGCGCT GCGTCTGTAT 
CTGGAGGCCT TCAGACTGCC AGGAGAGGCT CCGGTCATCC ATAGACTTTT GGAAACCTTC 
ACAGACACCT GGCATAAAGT GAACGGCTCT CCCTTCATGA CCAATGACGC AGGCTTCGCC 
CTGGCCTATG CTGTCATCAT GCTAAACACG GACCAGCACA ACAACAACGT GCGCAAACAG 
AACATCCCCA TGACCATTGA GCAATTCAAG AAGAATCTGA AGGGTGTGAA TGGAAATACA 
GACTTTGATC AGGATATGCT GGAAGACATC TACAATGCAA TCAAGAATGA AGAGATAGTG 
ATGCCAGACG AGCAGTCTGG CCTGGTTAAG GAGAACTATG TATGGAGCGT GCTGCTCCAC 
CGGGGGGCCA CCTCCGAGGG GGTCTTCCTT CATGTGCCCG CCGGCAGCTA CGACCACGAC 
CTGTTCACCA TGACCTGGGG GCCCACCATC GCAGCCCTCT CCTACGTCTT CGACAAGAGC 
CAGGACGACA CAATCATCCA GAAGGCCATT GCTGGCTTTA GGAAGTGTGC CATGATATCA 
GCTCATTATG GATTCAGTGA CGTTTTTGAT AATTTGATCA TCTCCCTCTG CAAGTTTACA 
ATACTCAGCA GTGAGTCGGT GGAGAACCTG CCCACAGTAT TTGGGAGTAA CGGGAAGGCA 
CAGATAGCGG CGAAAACGAT GTTCCACCTG GCCCACCGGC ACGGTGACAT CCTGAGGGAA 
GGCTGGAAGA ACATCATGGA CTCCATGCTG CAGCTGTTCA GAGCCGAGCT GCTGCCCAAG 
GCCATGCTGG AGGTGGAGGA TTTTGTGGAG CCAAATGGAA CGATTTCCCT TCAAAGAGAA 
GAGACACCGT CAAATCGGGG TGAGTCTGCG GTGCTGAGCT TTGTGAACTG GCTGACACTG 
AGTGGAGCGG AGCAGTCTGG TCAACGTGGG CCCTCTACTG AGAACCAGGA GGCCAAGCAG 
GCAGCCGTGC TCTGTATCAA GCAATGTGAC CCAGAGAAGC TGATCACAGA GAGTAAATTC 
CTGCAGCTGG AGTCTCTACA GGAGCTGATG AAGGCCCTGA TCTCAGTCAC ACCTGATGAG 
GAGACGTATG ACGAGGAGGA TGCTGCGTTC TGCCTGGAGA TGCTGTTACG CATAGTCATG 
GAGAACAGGG ACCGTGTGTC GTGTGTATGG CAGACGGTGC GGGATCACCT GTGCCACCTG 
TGTGTCCAGG CTACAGAGAG CTGCTTCCTG GTGGAGAGGG CCGTGGTGGG CCTCATAAGA 
CTGGCCATCC GCCTGCTGCG CAGAGAGGAC ATCAGCTCCC AGGTGCTGCT GTCCCTGCGT 
CTCCTGCTGA TGATGAAACC ACACGTCCTG GCCCGGGTTA GCCGGGAGGT GGCCTACGGC 
CTCCATGAGC TGCTTAAGAC CAACGCTGCC AACATCCACT GCACAGACGA CTGGTTCACC 
CTTTTCTCCC TAATCGAGTG CATCGGGGCC GGGATCAAAC CCCCCGCCTC CTTCAAGGTC 
ACCTCCACTA CCCCAGACAT CGACACAGGT GCCCAGTCGG ACAGTGAGCT GTCATTCCAC 
CACCCCAGTG AGGTGAGTCT GGACCGGGGG TACACCTCAG ACTCTGAGGT CTACACGGAG 
CACGGAAAGT CCAGGATGCC CCGCTCTGTC ACTGACGTGG ACGTGACCAG CAGCGGATGG 
CTGGTGGTTG GGAAAGACGA CCTGGACATC AGCAAGGTAG CGGCTTCTGG CTCCAAGACC 
CTGCTAAACC CTCTAGTGAA CCAGTACAGC CTGACCCTGG GTCAAGACAT GGGTTTCCAC 
GACACCAAGT CTCTCATCAA GTGTGTGGAG TCCCTCTCCT TCATCGTACG CGACGCTGCA 
CACGTCACCC CCGAGAACTT TGAGCTGTGT GTCAAGGCCA TCCGGGTGTT TGTGGAGGCC 
AGCCTAAACG GAGGCTACAG GACCTATGAG AAGAAGAAGA ACCACAAGTA CGACTCCCAG 
AAGTCCCGTG TGAGGAGGAA GCCCCGGGAC AAGGAGGGGG CATCCCGCCG CGCCAAGGCC 
TCCAGCCAGA GGCCTTCCCG TTCCCATAGC GATGACGAGG AGGACGAGGG GGTCCCAGCC 
AGCTACCACA TGGTGTCATT ACAGGTTAGT CAGGACCTGT TAGATCTGAT GCACACGCTG 
CATACGAGGG CCTCCAGCAT CTACAGCTCC TGGGCAGAGG AGCAGCGTCG CCTGGAGCCT 
GCAGGGAAGA AGATTGAGGC CGACTCCCAG ACACTGTGGA CCAGCTGCTG GTGCCCACTG 
CTCCAAGGGA TAGCCTGGCT GTGCTGCGAT GCCAGGAGGC TGGTGCGAAT GCAGGCCCTG 
ACCTACCTCC AGAGGGCACT GTTGGTCCAT GACCTGCAGA CACTAGATGC TGTGGAGTGG 
GAGTCCTGCT TCAACAAGGT GCTGTTCCCC CTGCTGACCA AGCTTCTGGA CAATATTAGC 
CCTGCAGACG TGTGTGGCAT GGAGGAGACC AGGATGAGGG CTTGCACCCT GCTGTCAAAG 
GTGTTCCTGC AGCACCTCTC TCCCCTGTTG TCTCTGTCCA CCTTCGCTGC CCTGTGGCTC 
ACCATCCTGG ACTTCATGGA CAAGTACATG CACGCTGGCT CCAGTGACCT GCTGCTGGAG 
GCCATCCCAG AGTCTCTGAA GAACATGCTG CTGGTGATGG ACACAGCAGG AATCTTCCAC 
AGCGCTGACT CTCGTACGGG ATACTCTGAC CTGTGGGAGA TCACCTGGGA ACGCATCGAC 
TGCTTCCTGC CTAACCTCCG GGAGGAGCTC TTCAAACAGA CAGTCATACC ACAACCAGAT 
CCTGTTCCTA GTCCCCCAGT GGAGCTTGTC CAGCCGGCTG CCCCCTGCCC CCCAGCCTCG 
CCACCCCCAG CTCAGGCCCC ATCTCTTGTG CCAGTGCCAG CTGCCCCCAC AGAGCCAAAG 
ACCTCCAGCA GACCAGCGTC GCCCCAAGAT CCCCCACCAC CTAGTACCAA CGGAACAGAC 
AGATCATCTC CAGTACCCCC GTCGACTCCC CCCATGCCCA CCCCAGTGAA GATGTCCCCC 
CAGATCTCTA GCCCACTGAT CCAGTCCCCC ACGAGTCAGT CCCCTCTCAT CCTGCAGCCC 
CTGGCCTCCC ATCTGCAGGT GGGGGGCGTG CCCCCTATGT CCCTCCCCAT TATTCTCAAC 
CCCGCCCTCA TTGAGGCCAG TTGCCCTGCT CCCCGCCCGC CACCCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014058135.1
CDS	31..5601
Translation	MVDKNIYIVQGEICTVVGAIKRNSRWNTHTLLDEEQDPLLNSFSHLKEILNNMKELSDVEPNVFLRPFLEVVRSEDTTGPITGLALTSVNKFLSYGLIDANHEAAAEAIENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFSRLPQFKEEAKSFVGANMKKLKMRAGGMSESSKWKKQKRSPHPPRHMVRSASGGLEQAPTTTLSNNLLSGGVPFIEQDSLSVSDSVASSMSIPTDSGLDTCSKATSREDLTDLDQSSSAATTPSTEPGRLYTQSEGRQVEHAQSASVESIPEVLEDGESVTEHSDSASLHDMDYVNPRGVRFTPQQTQRDGEDGAALIPYSLPCLRELFRFLISLTNPHDRHNTDAMMHMGLQLLTVALEAAHIAPYQSLLGLVKDELCRHLFQLLSVDRMNLYAASVRACFLLFESMRGHLKFQLEMYLKKLMDIITSENMKMLYEMKEMALEALVQLSRIPSFVTELYINYDCDFYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLEALLTVIDSTEAHCQAKVLNSSAQQDQSAEMVLAEGEGSTNDRTDTTTEASRQVNSANGQNVPEAHSWPLPPTSGHLMAEKMRLGRQDQEETEPAEKKEPKKPQRFSSCLPDSQELLDIRNKKKLLNSGTEQFNQKPKKGIQFLQEKGLLSSPMDNNEVAQWLRENPRLDKKQIGEFISDRKYMELLDSFVNTFTFRGLRIDEALRLYLEAFRLPGEAPVIHRLLETFTDTWHKVNGSPFMTNDAGFALAYAVIMLNTDQHNNNVRKQNIPMTIEQFKKNLKGVNGNTDFDQDMLEDIYNAIKNEEIVMPDEQSGLVKENYVWSVLLHRGATSEGVFLHVPAGSYDHDLFTMTWGPTIAALSYVFDKSQDDTIIQKAIAGFRKCAMISAHYGFSDVFDNLIISLCKFTILSSESVENLPTVFGSNGKAQIAAKTMFHLAHRHGDILREGWKNIMDSMLQLFRAELLPKAMLEVEDFVEPNGTISLQREETPSNRGESAVLSFVNWLTLSGAEQSGQRGPSTENQEAKQAAVLCIKQCDPEKLITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVMENRDRVSCVWQTVRDHLCHLCVQATESCFLVERAVVGLIRLAIRLLRREDISSQVLLSLRLLLMMKPHVLARVSREVAYGLHELLKTNAANIHCTDDWFTLFSLIECIGAGIKPPASFKVTSTTPDIDTGAQSDSELSFHHPSEVSLDRGYTSDSEVYTEHGKSRMPRSVTDVDVTSSGWLVVGKDDLDISKVAASGSKTLLNPLVNQYSLTLGQDMGFHDTKSLIKCVESLSFIVRDAAHVTPENFELCVKAIRVFVEASLNGGYRTYEKKKNHKYDSQKSRVRRKPRDKEGASRRAKASSQRPSRSHSDDEEDEGVPASYHMVSLQVSQDLLDLMHTLHTRASSIYSSWAEEQRRLEPAGKKIEADSQTLWTSCWCPLLQGIAWLCCDARRLVRMQALTYLQRALLVHDLQTLDAVEWESCFNKVLFPLLTKLLDNISPADVCGMEETRMRACTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLLEAIPESLKNMLLVMDTAGIFHSADSRTGYSDLWEITWERIDCFLPNLREELFKQTVIPQPDPVPSPPVELVQPAAPCPPASPPPAQAPSLVPVPAAPTEPKTSSRPASPQDPPPPSTNGTDRSSPVPPSTPPMPTPVKMSPQISSPLIQSPTSQSPLILQPLASHLQVGGVPPMSLPIILNPALIEASCPAPRPPPH

Target ORF information:

RefSeq Version	XM_014202660.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014202660.1

ORF Insert Sequence:

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGAGGATGG TGCGGCTCTC ATCCCCTACT CCCTGCCCTG TCTGCGGGAG 
CTCTTCCGCT TCCTCATCTC ACTGACCAAC CCCCATGACC GCCACAACAC GGACGCCATG 
ATGCACATGG GCCTGCAGTT GCTGACCGTG GCTCTGGAGG CGGCTCACAT CGCCCCCTAC 
CAGTCACTGC TGGGCCTGGT CAAAGACGAG CTCTGCAGAC ACCTCTTCCA GTTGCTAAGT 
GTGGACCGTA TGAACCTGTA TGCCGCCTCC GTCCGAGCGT GCTTCTTGCT CTTTGAGAGC 
ATGCGAGGAC ATTTGAAGTT TCAGCTCGAG ATGTATCTGA AGAAGCTGAT GGACATCATC 
ACATCAGAGA ACATGAAGAT GCTCTATGAG ATGAAGGAGA TGGCCCTGGA GGCCCTGGTG 
CAGCTCTCGA GGATCCCCAG CTTCGTCACC GAGCTCTACA TCAATTACGA CTGTGACTTC 
TACTGCTCAA ACCTGTTTGA AGACCTCACC AAGCTGCTCT CTAAGAATGC CTTCCCTGTG 
TCCGGACAGC TCTACACAAC CCACCTCCTC TCACTAGAGG CCCTACTGAC TGTTATTGAC 
AGCACTGAGG CCCACTGCCA GGCCAAGGTG CTCAACAGCA GCGCTCAGCA GGACCAATCA 
GCAGAAATGG TGTTGGCAGA AGGGGAGGGC TCAACCAACG ACCGTACAGA CACAACCACG 
GAAGCGAGCA GGCAGGTGAA CAGTGCCAAC GGTCAGAATG TTCCAGAGGC TCACAGTTGG 
CCCCTACCAC CTACCAGCGG GCACCTGATG GCAGAGAAGA TGAGACTGGG CAGACAAGAC 
CAGGAGGAGA CAGAACCAGC TGAGAAGAAA GAACCAAAAA AGCCACAACG CTTCTCGTCT 
TGTTTACCTG ATTCTCAAGA GCTGTTGGAC ATCAGAAATA AAAAGAAGCT GCTGAACAGC 
GGTACAGAGC AGTTCAACCA GAAGCCTAAG AAGGGCATCC AGTTCCTGCA GGAGAAGGGT 
CTCCTCAGCA GCCCCATGGA CAACAACGAG GTGGCCCAGT GGCTCCGGGA GAACCCCCGC 
CTCGACAAGA AGCAGATCGG AGAGTTCATC AGTGACCGCA AATACATGGA GTTACTGGAC 
AGCTTCGTCA ACACGTTCAC CTTCCGAGGG CTGCGTATTG ACGAGGCGCT GCGTCTGTAT 
CTGGAGGCCT TCAGACTGCC AGGAGAGGCT CCGGTCATCC ATAGACTTTT GGAAACCTTC 
ACAGACACCT GGCATAAAGT GAACGGCTCT CCCTTCATGA CCAATGACGC AGGCTTCGCC 
CTGGCCTATG CTGTCATCAT GCTAAACACG GACCAGCACA ACAACAACGT GCGCAAACAG 
AACATCCCCA TGACCATTGA GCAATTCAAG AAGAATCTGA AGGGTGTGAA TGGAAATACA 
GACTTTGATC AGGATATGCT GGAAGACATC TACAATGCAA TCAAGAATGA AGAGATAGTG 
ATGCCAGACG AGCAGTCTGG CCTGGTTAAG GAGAACTATG TATGGAGCGT GCTGCTCCAC 
CGGGGGGCCA CCTCCGAGGG GGTCTTCCTT CATGTGCCCG CCGGCAGCTA CGACCACGAC 
CTGTTCACCA TGACCTGGGG GCCCACCATC GCAGCCCTCT CCTACGTCTT CGACAAGAGC 
CAGGACGACA CAATCATCCA GAAGGCCATT GCTGGCTTTA GGAAGTGTGC CATGATATCA 
GCTCATTATG GATTCAGTGA CGTTTTTGAT AATTTGATCA TCTCCCTCTG CAAGTTTACA 
ATACTCAGCA GTGAGTCGGT GGAGAACCTG CCCACAGTAT TTGGGAGTAA CGGGAAGGCA 
CAGATAGCGG CGAAAACGAT GTTCCACCTG GCCCACCGGC ACGGTGACAT CCTGAGGGAA 
GGCTGGAAGA ACATCATGGA CTCCATGCTG CAGCTGTTCA GAGCCGAGCT GCTGCCCAAG 
GCCATGCTGG AGGTGGAGGA TTTTGTGGAG CCAAATGGAA CGATTTCCCT TCAAAGAGAA 
GAGACACCGT CAAATCGGGG TGAGTCTGCG GTGCTGAGCT TTGTGAACTG GCTGACACTG 
AGTGGAGCGG AGCAGTCTGG TCAACGTGGG CCCTCTACTG AGAACCAGGA GGCCAAGCAG 
GCAGCCGTGC TCTGTATCAA GCAATGTGAC CCAGAGAAGC TGATCACAGA GAGTAAATTC 
CTGCAGCTGG AGTCTCTACA GGAGCTGATG AAGGCCCTGA TCTCAGTCAC ACCTGATGAG 
GAGACGTATG ACGAGGAGGA TGCTGCGTTC TGCCTGGAGA TGCTGTTACG CATAGTCATG 
GAGAACAGGG ACCGTGTGTC GTGTGTATGG CAGACGGTGC GGGATCACCT GTGCCACCTG 
TGTGTCCAGG CTACAGAGAG CTGCTTCCTG GTGGAGAGGG CCGTGGTGGG CCTCATAAGA 
CTGGCCATCC GCCTGCTGCG CAGAGAGGAC ATCAGCTCCC AGGTGCTGCT GTCCCTGCGT 
CTCCTGCTGA TGATGAAACC ACACGTCCTG GCCCGGGTTA GCCGGGAGGT GGCCTACGGC 
CTCCATGAGC TGCTTAAGAC CAACGCTGCC AACATCCACT GCACAGACGA CTGGTTCACC 
CTTTTCTCCC TAATCGAGTG CATCGGGGCC GGGATCAAAC CCCCCGCCTC CTTCAAGGTC 
ACCTCCACTA CCCCAGACAT CGACACAGGT GCCCAGTCGG ACAGTGAGCT GTCATTCCAC 
CACCCCAGTG AGGTGAGTCT GGACCGGGGG TACACCTCAG ACTCTGAGGT CTACACGGAG 
CACGGAAAGT CCAGGATGCC CCGCTCTGTC ACTGACGTGG ACGTGACCAG CAGCGGATGG 
CTGGTGGTTG GGAAAGACGA CCTGGACATC AGCAAGGTAG CGGCTTCTGG CTCCAAGACC 
CTGCTAAACC CTCTAGTGAA CCAGTACAGC CTGACCCTGG GTCAAGACAT GGGTTTCCAC 
GACACCAAGT CTCTCATCAA GTGTGTGGAG TCCCTCTCCT TCATCGTACG CGACGCTGCA 
CACGTCACCC CCGAGAACTT TGAGCTGTGT GTCAAGGCCA TCCGGGTGTT TGTGGAGGCC 
AGCCTAAACG GAGGCTACAG GACCTATGAG AAGAAGAAGA ACCACAAGTA CGACTCCCAG 
AAGTCCCGTG TGAGGAGGAA GCCCCGGGAC AAGGAGGGGG CATCCCGCCG CGCCAAGGCC 
TCCAGCCAGA GGCCTTCCCG TTCCCATAGC GATGACGAGG AGGACGAGGG GGTCCCAGCC 
AGCTACCACA TGGTGTCATT ACAGGTTAGT CAGGACCTGT TAGATCTGAT GCACACGCTG 
CATACGAGGG CCTCCAGCAT CTACAGCTCC TGGGCAGAGG AGCAGCGTCG CCTGGAGCCT 
GCAGGGAAGA AGATTGAGGC CGACTCCCAG ACACTGTGGA CCAGCTGCTG GTGCCCACTG 
CTCCAAGGGA TAGCCTGGCT GTGCTGCGAT GCCAGGAGGC TGGTGCGAAT GCAGGCCCTG 
ACCTACCTCC AGAGGGCACT GTTGGTCCAT GACCTGCAGA CACTAGATGC TGTGGAGTGG 
GAGTCCTGCT TCAACAAGGT GCTGTTCCCC CTGCTGACCA AGCTTCTGGA CAATATTAGC 
CCTGCAGACG TGTGTGGCAT GGAGGAGACC AGGATGAGGG CTTGCACCCT GCTGTCAAAG 
GTGTTCCTGC AGCACCTCTC TCCCCTGTTG TCTCTGTCCA CCTTCGCTGC CCTGTGGCTC 
ACCATCCTGG ACTTCATGGA CAAGTACATG CACGCTGGCT CCAGTGACCT GCTGCTGGAG 
GCCATCCCAG AGTCTCTGAA GAACATGCTG CTGGTGATGG ACACAGCAGG AATCTTCCAC 
AGCGCTGACT CTCGTACGGG ATACTCTGAC CTGTGGGAGA TCACCTGGGA ACGCATCGAC 
TGCTTCCTGC CTAACCTCCG GGAGGAGCTC TTCAAACAGA CAGTCATACC ACAACCAGAT 
CCTGTTCCTA GTCCCCCAGT GGAGCTTGTC CAGCCGGCTG CCCCCTGCCC CCCAGCCTCG 
CCACCCCCAG CTCAGGCCCC ATCTCTTGTG CCAGTGCCAG CTGCCCCCAC AGAGCCAAAG 
ACCTCCAGCA GACCAGCGTC GCCCCAAGAT CCCCCACCAC CTAGTACCAA CGGAACAGAC 
AGATCATCTC CAGTACCCCC GTCGACTCCC CCCATGCCCA CCCCAGTGAA GATGTCCCCC 
CAGATCTCTA GCCCACTGAT CCAGTCCCCC ACGAGTCAGT CCCCTCTCAT CCTGCAGCCC 
CTGGCCTCCC ATCTGCAGGT GGGGGGCGTG CCCCCTATGT CCCTCCCCAT TATTCTCAAC 
CCCGCCCTCA TTGAGGCCAG TTGCCCTGCT CCCCGCCCGC CACCCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106606442 ( XM_014202656.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014058131.1 Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X1, mRNA.

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CloneID	OAt05303
Clone ID Related Accession (Same CDS sequence)	XM_014202660.1 , XM_014202656.1
Accession Version	XM_014202656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012332512.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGAGGATGG TGCGGCTCTC ATCCCCTACT CCCTGCCCTG TCTGCGGGAG 
CTCTTCCGCT TCCTCATCTC ACTGACCAAC CCCCATGACC GCCACAACAC GGACGCCATG 
ATGCACATGG GCCTGCAGTT GCTGACCGTG GCTCTGGAGG CGGCTCACAT CGCCCCCTAC 
CAGTCACTGC TGGGCCTGGT CAAAGACGAG CTCTGCAGAC ACCTCTTCCA GTTGCTAAGT 
GTGGACCGTA TGAACCTGTA TGCCGCCTCC GTCCGAGCGT GCTTCTTGCT CTTTGAGAGC 
ATGCGAGGAC ATTTGAAGTT TCAGCTCGAG ATGTATCTGA AGAAGCTGAT GGACATCATC 
ACATCAGAGA ACATGAAGAT GCTCTATGAG ATGAAGGAGA TGGCCCTGGA GGCCCTGGTG 
CAGCTCTCGA GGATCCCCAG CTTCGTCACC GAGCTCTACA TCAATTACGA CTGTGACTTC 
TACTGCTCAA ACCTGTTTGA AGACCTCACC AAGCTGCTCT CTAAGAATGC CTTCCCTGTG 
TCCGGACAGC TCTACACAAC CCACCTCCTC TCACTAGAGG CCCTACTGAC TGTTATTGAC 
AGCACTGAGG CCCACTGCCA GGCCAAGGTG CTCAACAGCA GCGCTCAGCA GGACCAATCA 
GCAGAAATGG TGTTGGCAGA AGGGGAGGGC TCAACCAACG ACCGTACAGA CACAACCACG 
GAAGCGAGCA GGCAGGTGAA CAGTGCCAAC GGTCAGAATG TTCCAGAGGC TCACAGTTGG 
CCCCTACCAC CTACCAGCGG GCACCTGATG GCAGAGAAGA TGAGACTGGG CAGACAAGAC 
CAGGAGGAGA CAGAACCAGC TGAGAAGAAA GAACCAAAAA AGCCACAACG CTTCTCGTCT 
TGTTTACCTG ATTCTCAAGA GCTGTTGGAC ATCAGAAATA AAAAGAAGCT GCTGAACAGC 
GGTACAGAGC AGTTCAACCA GAAGCCTAAG AAGGGCATCC AGTTCCTGCA GGAGAAGGGT 
CTCCTCAGCA GCCCCATGGA CAACAACGAG GTGGCCCAGT GGCTCCGGGA GAACCCCCGC 
CTCGACAAGA AGCAGATCGG AGAGTTCATC AGTGACCGCA AATACATGGA GTTACTGGAC 
AGCTTCGTCA ACACGTTCAC CTTCCGAGGG CTGCGTATTG ACGAGGCGCT GCGTCTGTAT 
CTGGAGGCCT TCAGACTGCC AGGAGAGGCT CCGGTCATCC ATAGACTTTT GGAAACCTTC 
ACAGACACCT GGCATAAAGT GAACGGCTCT CCCTTCATGA CCAATGACGC AGGCTTCGCC 
CTGGCCTATG CTGTCATCAT GCTAAACACG GACCAGCACA ACAACAACGT GCGCAAACAG 
AACATCCCCA TGACCATTGA GCAATTCAAG AAGAATCTGA AGGGTGTGAA TGGAAATACA 
GACTTTGATC AGGATATGCT GGAAGACATC TACAATGCAA TCAAGAATGA AGAGATAGTG 
ATGCCAGACG AGCAGTCTGG CCTGGTTAAG GAGAACTATG TATGGAGCGT GCTGCTCCAC 
CGGGGGGCCA CCTCCGAGGG GGTCTTCCTT CATGTGCCCG CCGGCAGCTA CGACCACGAC 
CTGTTCACCA TGACCTGGGG GCCCACCATC GCAGCCCTCT CCTACGTCTT CGACAAGAGC 
CAGGACGACA CAATCATCCA GAAGGCCATT GCTGGCTTTA GGAAGTGTGC CATGATATCA 
GCTCATTATG GATTCAGTGA CGTTTTTGAT AATTTGATCA TCTCCCTCTG CAAGTTTACA 
ATACTCAGCA GTGAGTCGGT GGAGAACCTG CCCACAGTAT TTGGGAGTAA CGGGAAGGCA 
CAGATAGCGG CGAAAACGAT GTTCCACCTG GCCCACCGGC ACGGTGACAT CCTGAGGGAA 
GGCTGGAAGA ACATCATGGA CTCCATGCTG CAGCTGTTCA GAGCCGAGCT GCTGCCCAAG 
GCCATGCTGG AGGTGGAGGA TTTTGTGGAG CCAAATGGAA CGATTTCCCT TCAAAGAGAA 
GAGACACCGT CAAATCGGGG TGAGTCTGCG GTGCTGAGCT TTGTGAACTG GCTGACACTG 
AGTGGAGCGG AGCAGTCTGG TCAACGTGGG CCCTCTACTG AGAACCAGGA GGCCAAGCAG 
GCAGCCGTGC TCTGTATCAA GCAATGTGAC CCAGAGAAGC TGATCACAGA GAGTAAATTC 
CTGCAGCTGG AGTCTCTACA GGAGCTGATG AAGGCCCTGA TCTCAGTCAC ACCTGATGAG 
GAGACGTATG ACGAGGAGGA TGCTGCGTTC TGCCTGGAGA TGCTGTTACG CATAGTCATG 
GAGAACAGGG ACCGTGTGTC GTGTGTATGG CAGACGGTGC GGGATCACCT GTGCCACCTG 
TGTGTCCAGG CTACAGAGAG CTGCTTCCTG GTGGAGAGGG CCGTGGTGGG CCTCATAAGA 
CTGGCCATCC GCCTGCTGCG CAGAGAGGAC ATCAGCTCCC AGGTGCTGCT GTCCCTGCGT 
CTCCTGCTGA TGATGAAACC ACACGTCCTG GCCCGGGTTA GCCGGGAGGT GGCCTACGGC 
CTCCATGAGC TGCTTAAGAC CAACGCTGCC AACATCCACT GCACAGACGA CTGGTTCACC 
CTTTTCTCCC TAATCGAGTG CATCGGGGCC GGGATCAAAC CCCCCGCCTC CTTCAAGGTC 
ACCTCCACTA CCCCAGACAT CGACACAGGT GCCCAGTCGG ACAGTGAGCT GTCATTCCAC 
CACCCCAGTG AGGTGAGTCT GGACCGGGGG TACACCTCAG ACTCTGAGGT CTACACGGAG 
CACGGAAAGT CCAGGATGCC CCGCTCTGTC ACTGACGTGG ACGTGACCAG CAGCGGATGG 
CTGGTGGTTG GGAAAGACGA CCTGGACATC AGCAAGGTAG CGGCTTCTGG CTCCAAGACC 
CTGCTAAACC CTCTAGTGAA CCAGTACAGC CTGACCCTGG GTCAAGACAT GGGTTTCCAC 
GACACCAAGT CTCTCATCAA GTGTGTGGAG TCCCTCTCCT TCATCGTACG CGACGCTGCA 
CACGTCACCC CCGAGAACTT TGAGCTGTGT GTCAAGGCCA TCCGGGTGTT TGTGGAGGCC 
AGCCTAAACG GAGGCTACAG GACCTATGAG AAGAAGAAGA ACCACAAGTA CGACTCCCAG 
AAGTCCCGTG TGAGGAGGAA GCCCCGGGAC AAGGAGGGGG CATCCCGCCG CGCCAAGGCC 
TCCAGCCAGA GGCCTTCCCG TTCCCATAGC GATGACGAGG AGGACGAGGG GGTCCCAGCC 
AGCTACCACA TGGTGTCATT ACAGGTTAGT CAGGACCTGT TAGATCTGAT GCACACGCTG 
CATACGAGGG CCTCCAGCAT CTACAGCTCC TGGGCAGAGG AGCAGCGTCG CCTGGAGCCT 
GCAGGGAAGA AGATTGAGGC CGACTCCCAG ACACTGTGGA CCAGCTGCTG GTGCCCACTG 
CTCCAAGGGA TAGCCTGGCT GTGCTGCGAT GCCAGGAGGC TGGTGCGAAT GCAGGCCCTG 
ACCTACCTCC AGAGGGCACT GTTGGTCCAT GACCTGCAGA CACTAGATGC TGTGGAGTGG 
GAGTCCTGCT TCAACAAGGT GCTGTTCCCC CTGCTGACCA AGCTTCTGGA CAATATTAGC 
CCTGCAGACG TGTGTGGCAT GGAGGAGACC AGGATGAGGG CTTGCACCCT GCTGTCAAAG 
GTGTTCCTGC AGCACCTCTC TCCCCTGTTG TCTCTGTCCA CCTTCGCTGC CCTGTGGCTC 
ACCATCCTGG ACTTCATGGA CAAGTACATG CACGCTGGCT CCAGTGACCT GCTGCTGGAG 
GCCATCCCAG AGTCTCTGAA GAACATGCTG CTGGTGATGG ACACAGCAGG AATCTTCCAC 
AGCGCTGACT CTCGTACGGG ATACTCTGAC CTGTGGGAGA TCACCTGGGA ACGCATCGAC 
TGCTTCCTGC CTAACCTCCG GGAGGAGCTC TTCAAACAGA CAGTCATACC ACAACCAGAT 
CCTGTTCCTA GTCCCCCAGT GGAGCTTGTC CAGCCGGCTG CCCCCTGCCC CCCAGCCTCG 
CCACCCCCAG CTCAGGCCCC ATCTCTTGTG CCAGTGCCAG CTGCCCCCAC AGAGCCAAAG 
ACCTCCAGCA GACCAGCGTC GCCCCAAGAT CCCCCACCAC CTAGTACCAA CGGAACAGAC 
AGATCATCTC CAGTACCCCC GTCGACTCCC CCCATGCCCA CCCCAGTGAA GATGTCCCCC 
CAGATCTCTA GCCCACTGAT CCAGTCCCCC ACGAGTCAGT CCCCTCTCAT CCTGCAGCCC 
CTGGCCTCCC ATCTGCAGGT GGGGGGCGTG CCCCCTATGT CCCTCCCCAT TATTCTCAAC 
CCCGCCCTCA TTGAGGCCAG TTGCCCTGCT CCCCGCCCGC CACCCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014058131.1
CDS	285..5855
Translation	MVDKNIYIVQGEICTVVGAIKRNSRWNTHTLLDEEQDPLLNSFSHLKEILNNMKELSDVEPNVFLRPFLEVVRSEDTTGPITGLALTSVNKFLSYGLIDANHEAAAEAIENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFSRLPQFKEEAKSFVGANMKKLKMRAGGMSESSKWKKQKRSPHPPRHMVRSASGGLEQAPTTTLSNNLLSGGVPFIEQDSLSVSDSVASSMSIPTDSGLDTCSKATSREDLTDLDQSSSAATTPSTEPGRLYTQSEGRQVEHAQSASVESIPEVLEDGESVTEHSDSASLHDMDYVNPRGVRFTPQQTQRDGEDGAALIPYSLPCLRELFRFLISLTNPHDRHNTDAMMHMGLQLLTVALEAAHIAPYQSLLGLVKDELCRHLFQLLSVDRMNLYAASVRACFLLFESMRGHLKFQLEMYLKKLMDIITSENMKMLYEMKEMALEALVQLSRIPSFVTELYINYDCDFYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLEALLTVIDSTEAHCQAKVLNSSAQQDQSAEMVLAEGEGSTNDRTDTTTEASRQVNSANGQNVPEAHSWPLPPTSGHLMAEKMRLGRQDQEETEPAEKKEPKKPQRFSSCLPDSQELLDIRNKKKLLNSGTEQFNQKPKKGIQFLQEKGLLSSPMDNNEVAQWLRENPRLDKKQIGEFISDRKYMELLDSFVNTFTFRGLRIDEALRLYLEAFRLPGEAPVIHRLLETFTDTWHKVNGSPFMTNDAGFALAYAVIMLNTDQHNNNVRKQNIPMTIEQFKKNLKGVNGNTDFDQDMLEDIYNAIKNEEIVMPDEQSGLVKENYVWSVLLHRGATSEGVFLHVPAGSYDHDLFTMTWGPTIAALSYVFDKSQDDTIIQKAIAGFRKCAMISAHYGFSDVFDNLIISLCKFTILSSESVENLPTVFGSNGKAQIAAKTMFHLAHRHGDILREGWKNIMDSMLQLFRAELLPKAMLEVEDFVEPNGTISLQREETPSNRGESAVLSFVNWLTLSGAEQSGQRGPSTENQEAKQAAVLCIKQCDPEKLITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVMENRDRVSCVWQTVRDHLCHLCVQATESCFLVERAVVGLIRLAIRLLRREDISSQVLLSLRLLLMMKPHVLARVSREVAYGLHELLKTNAANIHCTDDWFTLFSLIECIGAGIKPPASFKVTSTTPDIDTGAQSDSELSFHHPSEVSLDRGYTSDSEVYTEHGKSRMPRSVTDVDVTSSGWLVVGKDDLDISKVAASGSKTLLNPLVNQYSLTLGQDMGFHDTKSLIKCVESLSFIVRDAAHVTPENFELCVKAIRVFVEASLNGGYRTYEKKKNHKYDSQKSRVRRKPRDKEGASRRAKASSQRPSRSHSDDEEDEGVPASYHMVSLQVSQDLLDLMHTLHTRASSIYSSWAEEQRRLEPAGKKIEADSQTLWTSCWCPLLQGIAWLCCDARRLVRMQALTYLQRALLVHDLQTLDAVEWESCFNKVLFPLLTKLLDNISPADVCGMEETRMRACTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLLEAIPESLKNMLLVMDTAGIFHSADSRTGYSDLWEITWERIDCFLPNLREELFKQTVIPQPDPVPSPPVELVQPAAPCPPASPPPAQAPSLVPVPAAPTEPKTSSRPASPQDPPPPSTNGTDRSSPVPPSTPPMPTPVKMSPQISSPLIQSPTSQSPLILQPLASHLQVGGVPPMSLPIILNPALIEASCPAPRPPPH

Target ORF information:

RefSeq Version	XM_014202656.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014202656.1

ORF Insert Sequence:

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGAGGATGG TGCGGCTCTC ATCCCCTACT CCCTGCCCTG TCTGCGGGAG 
CTCTTCCGCT TCCTCATCTC ACTGACCAAC CCCCATGACC GCCACAACAC GGACGCCATG 
ATGCACATGG GCCTGCAGTT GCTGACCGTG GCTCTGGAGG CGGCTCACAT CGCCCCCTAC 
CAGTCACTGC TGGGCCTGGT CAAAGACGAG CTCTGCAGAC ACCTCTTCCA GTTGCTAAGT 
GTGGACCGTA TGAACCTGTA TGCCGCCTCC GTCCGAGCGT GCTTCTTGCT CTTTGAGAGC 
ATGCGAGGAC ATTTGAAGTT TCAGCTCGAG ATGTATCTGA AGAAGCTGAT GGACATCATC 
ACATCAGAGA ACATGAAGAT GCTCTATGAG ATGAAGGAGA TGGCCCTGGA GGCCCTGGTG 
CAGCTCTCGA GGATCCCCAG CTTCGTCACC GAGCTCTACA TCAATTACGA CTGTGACTTC 
TACTGCTCAA ACCTGTTTGA AGACCTCACC AAGCTGCTCT CTAAGAATGC CTTCCCTGTG 
TCCGGACAGC TCTACACAAC CCACCTCCTC TCACTAGAGG CCCTACTGAC TGTTATTGAC 
AGCACTGAGG CCCACTGCCA GGCCAAGGTG CTCAACAGCA GCGCTCAGCA GGACCAATCA 
GCAGAAATGG TGTTGGCAGA AGGGGAGGGC TCAACCAACG ACCGTACAGA CACAACCACG 
GAAGCGAGCA GGCAGGTGAA CAGTGCCAAC GGTCAGAATG TTCCAGAGGC TCACAGTTGG 
CCCCTACCAC CTACCAGCGG GCACCTGATG GCAGAGAAGA TGAGACTGGG CAGACAAGAC 
CAGGAGGAGA CAGAACCAGC TGAGAAGAAA GAACCAAAAA AGCCACAACG CTTCTCGTCT 
TGTTTACCTG ATTCTCAAGA GCTGTTGGAC ATCAGAAATA AAAAGAAGCT GCTGAACAGC 
GGTACAGAGC AGTTCAACCA GAAGCCTAAG AAGGGCATCC AGTTCCTGCA GGAGAAGGGT 
CTCCTCAGCA GCCCCATGGA CAACAACGAG GTGGCCCAGT GGCTCCGGGA GAACCCCCGC 
CTCGACAAGA AGCAGATCGG AGAGTTCATC AGTGACCGCA AATACATGGA GTTACTGGAC 
AGCTTCGTCA ACACGTTCAC CTTCCGAGGG CTGCGTATTG ACGAGGCGCT GCGTCTGTAT 
CTGGAGGCCT TCAGACTGCC AGGAGAGGCT CCGGTCATCC ATAGACTTTT GGAAACCTTC 
ACAGACACCT GGCATAAAGT GAACGGCTCT CCCTTCATGA CCAATGACGC AGGCTTCGCC 
CTGGCCTATG CTGTCATCAT GCTAAACACG GACCAGCACA ACAACAACGT GCGCAAACAG 
AACATCCCCA TGACCATTGA GCAATTCAAG AAGAATCTGA AGGGTGTGAA TGGAAATACA 
GACTTTGATC AGGATATGCT GGAAGACATC TACAATGCAA TCAAGAATGA AGAGATAGTG 
ATGCCAGACG AGCAGTCTGG CCTGGTTAAG GAGAACTATG TATGGAGCGT GCTGCTCCAC 
CGGGGGGCCA CCTCCGAGGG GGTCTTCCTT CATGTGCCCG CCGGCAGCTA CGACCACGAC 
CTGTTCACCA TGACCTGGGG GCCCACCATC GCAGCCCTCT CCTACGTCTT CGACAAGAGC 
CAGGACGACA CAATCATCCA GAAGGCCATT GCTGGCTTTA GGAAGTGTGC CATGATATCA 
GCTCATTATG GATTCAGTGA CGTTTTTGAT AATTTGATCA TCTCCCTCTG CAAGTTTACA 
ATACTCAGCA GTGAGTCGGT GGAGAACCTG CCCACAGTAT TTGGGAGTAA CGGGAAGGCA 
CAGATAGCGG CGAAAACGAT GTTCCACCTG GCCCACCGGC ACGGTGACAT CCTGAGGGAA 
GGCTGGAAGA ACATCATGGA CTCCATGCTG CAGCTGTTCA GAGCCGAGCT GCTGCCCAAG 
GCCATGCTGG AGGTGGAGGA TTTTGTGGAG CCAAATGGAA CGATTTCCCT TCAAAGAGAA 
GAGACACCGT CAAATCGGGG TGAGTCTGCG GTGCTGAGCT TTGTGAACTG GCTGACACTG 
AGTGGAGCGG AGCAGTCTGG TCAACGTGGG CCCTCTACTG AGAACCAGGA GGCCAAGCAG 
GCAGCCGTGC TCTGTATCAA GCAATGTGAC CCAGAGAAGC TGATCACAGA GAGTAAATTC 
CTGCAGCTGG AGTCTCTACA GGAGCTGATG AAGGCCCTGA TCTCAGTCAC ACCTGATGAG 
GAGACGTATG ACGAGGAGGA TGCTGCGTTC TGCCTGGAGA TGCTGTTACG CATAGTCATG 
GAGAACAGGG ACCGTGTGTC GTGTGTATGG CAGACGGTGC GGGATCACCT GTGCCACCTG 
TGTGTCCAGG CTACAGAGAG CTGCTTCCTG GTGGAGAGGG CCGTGGTGGG CCTCATAAGA 
CTGGCCATCC GCCTGCTGCG CAGAGAGGAC ATCAGCTCCC AGGTGCTGCT GTCCCTGCGT 
CTCCTGCTGA TGATGAAACC ACACGTCCTG GCCCGGGTTA GCCGGGAGGT GGCCTACGGC 
CTCCATGAGC TGCTTAAGAC CAACGCTGCC AACATCCACT GCACAGACGA CTGGTTCACC 
CTTTTCTCCC TAATCGAGTG CATCGGGGCC GGGATCAAAC CCCCCGCCTC CTTCAAGGTC 
ACCTCCACTA CCCCAGACAT CGACACAGGT GCCCAGTCGG ACAGTGAGCT GTCATTCCAC 
CACCCCAGTG AGGTGAGTCT GGACCGGGGG TACACCTCAG ACTCTGAGGT CTACACGGAG 
CACGGAAAGT CCAGGATGCC CCGCTCTGTC ACTGACGTGG ACGTGACCAG CAGCGGATGG 
CTGGTGGTTG GGAAAGACGA CCTGGACATC AGCAAGGTAG CGGCTTCTGG CTCCAAGACC 
CTGCTAAACC CTCTAGTGAA CCAGTACAGC CTGACCCTGG GTCAAGACAT GGGTTTCCAC 
GACACCAAGT CTCTCATCAA GTGTGTGGAG TCCCTCTCCT TCATCGTACG CGACGCTGCA 
CACGTCACCC CCGAGAACTT TGAGCTGTGT GTCAAGGCCA TCCGGGTGTT TGTGGAGGCC 
AGCCTAAACG GAGGCTACAG GACCTATGAG AAGAAGAAGA ACCACAAGTA CGACTCCCAG 
AAGTCCCGTG TGAGGAGGAA GCCCCGGGAC AAGGAGGGGG CATCCCGCCG CGCCAAGGCC 
TCCAGCCAGA GGCCTTCCCG TTCCCATAGC GATGACGAGG AGGACGAGGG GGTCCCAGCC 
AGCTACCACA TGGTGTCATT ACAGGTTAGT CAGGACCTGT TAGATCTGAT GCACACGCTG 
CATACGAGGG CCTCCAGCAT CTACAGCTCC TGGGCAGAGG AGCAGCGTCG CCTGGAGCCT 
GCAGGGAAGA AGATTGAGGC CGACTCCCAG ACACTGTGGA CCAGCTGCTG GTGCCCACTG 
CTCCAAGGGA TAGCCTGGCT GTGCTGCGAT GCCAGGAGGC TGGTGCGAAT GCAGGCCCTG 
ACCTACCTCC AGAGGGCACT GTTGGTCCAT GACCTGCAGA CACTAGATGC TGTGGAGTGG 
GAGTCCTGCT TCAACAAGGT GCTGTTCCCC CTGCTGACCA AGCTTCTGGA CAATATTAGC 
CCTGCAGACG TGTGTGGCAT GGAGGAGACC AGGATGAGGG CTTGCACCCT GCTGTCAAAG 
GTGTTCCTGC AGCACCTCTC TCCCCTGTTG TCTCTGTCCA CCTTCGCTGC CCTGTGGCTC 
ACCATCCTGG ACTTCATGGA CAAGTACATG CACGCTGGCT CCAGTGACCT GCTGCTGGAG 
GCCATCCCAG AGTCTCTGAA GAACATGCTG CTGGTGATGG ACACAGCAGG AATCTTCCAC 
AGCGCTGACT CTCGTACGGG ATACTCTGAC CTGTGGGAGA TCACCTGGGA ACGCATCGAC 
TGCTTCCTGC CTAACCTCCG GGAGGAGCTC TTCAAACAGA CAGTCATACC ACAACCAGAT 
CCTGTTCCTA GTCCCCCAGT GGAGCTTGTC CAGCCGGCTG CCCCCTGCCC CCCAGCCTCG 
CCACCCCCAG CTCAGGCCCC ATCTCTTGTG CCAGTGCCAG CTGCCCCCAC AGAGCCAAAG 
ACCTCCAGCA GACCAGCGTC GCCCCAAGAT CCCCCACCAC CTAGTACCAA CGGAACAGAC 
AGATCATCTC CAGTACCCCC GTCGACTCCC CCCATGCCCA CCCCAGTGAA GATGTCCCCC 
CAGATCTCTA GCCCACTGAT CCAGTCCCCC ACGAGTCAGT CCCCTCTCAT CCTGCAGCCC 
CTGGCCTCCC ATCTGCAGGT GGGGGGCGTG CCCCCTATGT CCCTCCCCAT TATTCTCAAC 
CCCGCCCTCA TTGAGGCCAG TTGCCCTGCT CCCCGCCCGC CACCCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106606442 ( XM_014202667.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014058142.1 Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X3, mRNA.

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CloneID	OAt05304
Clone ID Related Accession (Same CDS sequence)	XM_014202667.1
Accession Version	XM_014202667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012332512.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGCGGCTCT CATCCCCTAC TCCCTGCCCT GTCTGCGGGA GCTCTTCCGC 
TTCCTCATCT CACTGACCAA CCCCCATGAC CGCCACAACA CGGACGCCAT GATGCACATG 
GGCCTGCAGT TGCTGACCGT GGCTCTGGAG GCGGCTCACA TCGCCCCCTA CCAGTCACTG 
CTGGGCCTGG TCAAAGACGA GCTCTGCAGA CACCTCTTCC AGTTGCTAAG TGTGGACCGT 
ATGAACCTGT ATGCCGCCTC CGTCCGAGCG TGCTTCTTGC TCTTTGAGAG CATGCGAGGA 
CATTTGAAGT TTCAGCTCGA GATGTATCTG AAGAAGCTGA TGGACATCAT CACATCAGAG 
AACATGAAGA TGCTCTATGA GATGAAGGAG ATGGCCCTGG AGGCCCTGGT GCAGCTCTCG 
AGGATCCCCA GCTTCGTCAC CGAGCTCTAC ATCAATTACG ACTGTGACTT CTACTGCTCA 
AACCTGTTTG AAGACCTCAC CAAGCTGCTC TCTAAGAATG CCTTCCCTGT GTCCGGACAG 
CTCTACACAA CCCACCTCCT CTCACTAGAG GCCCTACTGA CTGTTATTGA CAGCACTGAG 
GCCCACTGCC AGGCCAAGGT GCTCAACAGC AGCGCTCAGC AGGACCAATC AGCAGAAATG 
GTGTTGGCAG AAGGGGAGGG CTCAACCAAC GACCGTACAG ACACAACCAC GGAAGCGAGC 
AGGCAGGTGA ACAGTGCCAA CGGTCAGAAT GTTCCAGAGG CTCACAGTTG GCCCCTACCA 
CCTACCAGCG GGCACCTGAT GGCAGAGAAG ATGAGACTGG GCAGACAAGA CCAGGAGGAG 
ACAGAACCAG CTGAGAAGAA AGAACCAAAA AAGCCACAAC GCTTCTCGTC TTGTTTACCT 
GATTCTCAAG AGCTGTTGGA CATCAGAAAT AAAAAGAAGC TGCTGAACAG CGGTACAGAG 
CAGTTCAACC AGAAGCCTAA GAAGGGCATC CAGTTCCTGC AGGAGAAGGG TCTCCTCAGC 
AGCCCCATGG ACAACAACGA GGTGGCCCAG TGGCTCCGGG AGAACCCCCG CCTCGACAAG 
AAGCAGATCG GAGAGTTCAT CAGTGACCGC AAATACATGG AGTTACTGGA CAGCTTCGTC 
AACACGTTCA CCTTCCGAGG GCTGCGTATT GACGAGGCGC TGCGTCTGTA TCTGGAGGCC 
TTCAGACTGC CAGGAGAGGC TCCGGTCATC CATAGACTTT TGGAAACCTT CACAGACACC 
TGGCATAAAG TGAACGGCTC TCCCTTCATG ACCAATGACG CAGGCTTCGC CCTGGCCTAT 
GCTGTCATCA TGCTAAACAC GGACCAGCAC AACAACAACG TGCGCAAACA GAACATCCCC 
ATGACCATTG AGCAATTCAA GAAGAATCTG AAGGGTGTGA ATGGAAATAC AGACTTTGAT 
CAGGATATGC TGGAAGACAT CTACAATGCA ATCAAGAATG AAGAGATAGT GATGCCAGAC 
GAGCAGTCTG GCCTGGTTAA GGAGAACTAT GTATGGAGCG TGCTGCTCCA CCGGGGGGCC 
ACCTCCGAGG GGGTCTTCCT TCATGTGCCC GCCGGCAGCT ACGACCACGA CCTGTTCACC 
ATGACCTGGG GGCCCACCAT CGCAGCCCTC TCCTACGTCT TCGACAAGAG CCAGGACGAC 
ACAATCATCC AGAAGGCCAT TGCTGGCTTT AGGAAGTGTG CCATGATATC AGCTCATTAT 
GGATTCAGTG ACGTTTTTGA TAATTTGATC ATCTCCCTCT GCAAGTTTAC AATACTCAGC 
AGTGAGTCGG TGGAGAACCT GCCCACAGTA TTTGGGAGTA ACGGGAAGGC ACAGATAGCG 
GCGAAAACGA TGTTCCACCT GGCCCACCGG CACGGTGACA TCCTGAGGGA AGGCTGGAAG 
AACATCATGG ACTCCATGCT GCAGCTGTTC AGAGCCGAGC TGCTGCCCAA GGCCATGCTG 
GAGGTGGAGG ATTTTGTGGA GCCAAATGGA ACGATTTCCC TTCAAAGAGA AGAGACACCG 
TCAAATCGGG GTGAGTCTGC GGTGCTGAGC TTTGTGAACT GGCTGACACT GAGTGGAGCG 
GAGCAGTCTG GTCAACGTGG GCCCTCTACT GAGAACCAGG AGGCCAAGCA GGCAGCCGTG 
CTCTGTATCA AGCAATGTGA CCCAGAGAAG CTGATCACAG AGAGTAAATT CCTGCAGCTG 
GAGTCTCTAC AGGAGCTGAT GAAGGCCCTG ATCTCAGTCA CACCTGATGA GGAGACGTAT 
GACGAGGAGG ATGCTGCGTT CTGCCTGGAG ATGCTGTTAC GCATAGTCAT GGAGAACAGG 
GACCGTGTGT CGTGTGTATG GCAGACGGTG CGGGATCACC TGTGCCACCT GTGTGTCCAG 
GCTACAGAGA GCTGCTTCCT GGTGGAGAGG GCCGTGGTGG GCCTCATAAG ACTGGCCATC 
CGCCTGCTGC GCAGAGAGGA CATCAGCTCC CAGGTGCTGC TGTCCCTGCG TCTCCTGCTG 
ATGATGAAAC CACACGTCCT GGCCCGGGTT AGCCGGGAGG TGGCCTACGG CCTCCATGAG 
CTGCTTAAGA CCAACGCTGC CAACATCCAC TGCACAGACG ACTGGTTCAC CCTTTTCTCC 
CTAATCGAGT GCATCGGGGC CGGGATCAAA CCCCCCGCCT CCTTCAAGGT CACCTCCACT 
ACCCCAGACA TCGACACAGG TGCCCAGTCG GACAGTGAGC TGTCATTCCA CCACCCCAGT 
GAGGTGAGTC TGGACCGGGG GTACACCTCA GACTCTGAGG TCTACACGGA GCACGGAAAG 
TCCAGGATGC CCCGCTCTGT CACTGACGTG GACGTGACCA GCAGCGGATG GCTGGTGGTT 
GGGAAAGACG ACCTGGACAT CAGCAAGGTA GCGGCTTCTG GCTCCAAGAC CCTGCTAAAC 
CCTCTAGTGA ACCAGTACAG CCTGACCCTG GGTCAAGACA TGGGTTTCCA CGACACCAAG 
TCTCTCATCA AGTGTGTGGA GTCCCTCTCC TTCATCGTAC GCGACGCTGC ACACGTCACC 
CCCGAGAACT TTGAGCTGTG TGTCAAGGCC ATCCGGGTGT TTGTGGAGGC CAGCCTAAAC 
GGAGGCTACA GGACCTATGA GAAGAAGAAG AACCACAAGT ACGACTCCCA GAAGTCCCGT 
GTGAGGAGGA AGCCCCGGGA CAAGGAGGGG GCATCCCGCC GCGCCAAGGC CTCCAGCCAG 
AGGCCTTCCC GTTCCCATAG CGATGACGAG GAGGACGAGG GGGTCCCAGC CAGCTACCAC 
ATGGTGTCAT TACAGGTTAG TCAGGACCTG TTAGATCTGA TGCACACGCT GCATACGAGG 
GCCTCCAGCA TCTACAGCTC CTGGGCAGAG GAGCAGCGTC GCCTGGAGCC TGCAGGGAAG 
AAGATTGAGG CCGACTCCCA GACACTGTGG ACCAGCTGCT GGTGCCCACT GCTCCAAGGG 
ATAGCCTGGC TGTGCTGCGA TGCCAGGAGG CTGGTGCGAA TGCAGGCCCT GACCTACCTC 
CAGAGGGCAC TGTTGGTCCA TGACCTGCAG ACACTAGATG CTGTGGAGTG GGAGTCCTGC 
TTCAACAAGG TGCTGTTCCC CCTGCTGACC AAGCTTCTGG ACAATATTAG CCCTGCAGAC 
GTGTGTGGCA TGGAGGAGAC CAGGATGAGG GCTTGCACCC TGCTGTCAAA GGTGTTCCTG 
CAGCACCTCT CTCCCCTGTT GTCTCTGTCC ACCTTCGCTG CCCTGTGGCT CACCATCCTG 
GACTTCATGG ACAAGTACAT GCACGCTGGC TCCAGTGACC TGCTGCTGGA GGCCATCCCA 
GAGTCTCTGA AGAACATGCT GCTGGTGATG GACACAGCAG GAATCTTCCA CAGCGCTGAC 
TCTCGTACGG GATACTCTGA CCTGTGGGAG ATCACCTGGG AACGCATCGA CTGCTTCCTG 
CCTAACCTCC GGGAGGAGCT CTTCAAACAG ACAGTCATAC CACAACCAGA TCCTGTTCCT 
AGTCCCCCAG TGGAGCTTGT CCAGCCGGCT GCCCCCTGCC CCCCAGCCTC GCCACCCCCA 
GCTCAGGCCC CATCTCTTGT GCCAGTGCCA GCTGCCCCCA CAGAGCCAAA GACCTCCAGC 
AGACCAGCGT CGCCCCAAGA TCCCCCACCA CCTAGTACCA ACGGAACAGA CAGATCATCT 
CCAGTACCCC CGTCGACTCC CCCCATGCCC ACCCCAGTGA AGATGTCCCC CCAGATCTCT 
AGCCCACTGA TCCAGTCCCC CACGAGTCAG TCCCCTCTCA TCCTGCAGCC CCTGGCCTCC 
CATCTGCAGG TGGGGGGCGT GCCCCCTATG TCCCTCCCCA TTATTCTCAA CCCCGCCCTC 
ATTGAGGCCA GTTGCCCTGC TCCCCGCCCG CCACCCCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014058142.1
CDS	285..5846
Translation	MVDKNIYIVQGEICTVVGAIKRNSRWNTHTLLDEEQDPLLNSFSHLKEILNNMKELSDVEPNVFLRPFLEVVRSEDTTGPITGLALTSVNKFLSYGLIDANHEAAAEAIENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFSRLPQFKEEAKSFVGANMKKLKMRAGGMSESSKWKKQKRSPHPPRHMVRSASGGLEQAPTTTLSNNLLSGGVPFIEQDSLSVSDSVASSMSIPTDSGLDTCSKATSREDLTDLDQSSSAATTPSTEPGRLYTQSEGRQVEHAQSASVESIPEVLEDGESVTEHSDSASLHDMDYVNPRGVRFTPQQTQRDGAALIPYSLPCLRELFRFLISLTNPHDRHNTDAMMHMGLQLLTVALEAAHIAPYQSLLGLVKDELCRHLFQLLSVDRMNLYAASVRACFLLFESMRGHLKFQLEMYLKKLMDIITSENMKMLYEMKEMALEALVQLSRIPSFVTELYINYDCDFYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLEALLTVIDSTEAHCQAKVLNSSAQQDQSAEMVLAEGEGSTNDRTDTTTEASRQVNSANGQNVPEAHSWPLPPTSGHLMAEKMRLGRQDQEETEPAEKKEPKKPQRFSSCLPDSQELLDIRNKKKLLNSGTEQFNQKPKKGIQFLQEKGLLSSPMDNNEVAQWLRENPRLDKKQIGEFISDRKYMELLDSFVNTFTFRGLRIDEALRLYLEAFRLPGEAPVIHRLLETFTDTWHKVNGSPFMTNDAGFALAYAVIMLNTDQHNNNVRKQNIPMTIEQFKKNLKGVNGNTDFDQDMLEDIYNAIKNEEIVMPDEQSGLVKENYVWSVLLHRGATSEGVFLHVPAGSYDHDLFTMTWGPTIAALSYVFDKSQDDTIIQKAIAGFRKCAMISAHYGFSDVFDNLIISLCKFTILSSESVENLPTVFGSNGKAQIAAKTMFHLAHRHGDILREGWKNIMDSMLQLFRAELLPKAMLEVEDFVEPNGTISLQREETPSNRGESAVLSFVNWLTLSGAEQSGQRGPSTENQEAKQAAVLCIKQCDPEKLITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVMENRDRVSCVWQTVRDHLCHLCVQATESCFLVERAVVGLIRLAIRLLRREDISSQVLLSLRLLLMMKPHVLARVSREVAYGLHELLKTNAANIHCTDDWFTLFSLIECIGAGIKPPASFKVTSTTPDIDTGAQSDSELSFHHPSEVSLDRGYTSDSEVYTEHGKSRMPRSVTDVDVTSSGWLVVGKDDLDISKVAASGSKTLLNPLVNQYSLTLGQDMGFHDTKSLIKCVESLSFIVRDAAHVTPENFELCVKAIRVFVEASLNGGYRTYEKKKNHKYDSQKSRVRRKPRDKEGASRRAKASSQRPSRSHSDDEEDEGVPASYHMVSLQVSQDLLDLMHTLHTRASSIYSSWAEEQRRLEPAGKKIEADSQTLWTSCWCPLLQGIAWLCCDARRLVRMQALTYLQRALLVHDLQTLDAVEWESCFNKVLFPLLTKLLDNISPADVCGMEETRMRACTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLLEAIPESLKNMLLVMDTAGIFHSADSRTGYSDLWEITWERIDCFLPNLREELFKQTVIPQPDPVPSPPVELVQPAAPCPPASPPPAQAPSLVPVPAAPTEPKTSSRPASPQDPPPPSTNGTDRSSPVPPSTPPMPTPVKMSPQISSPLIQSPTSQSPLILQPLASHLQVGGVPPMSLPIILNPALIEASCPAPRPPPH

Target ORF information:

RefSeq Version	XM_014202667.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014202667.1

ORF Insert Sequence:

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGCGGCTCT CATCCCCTAC TCCCTGCCCT GTCTGCGGGA GCTCTTCCGC 
TTCCTCATCT CACTGACCAA CCCCCATGAC CGCCACAACA CGGACGCCAT GATGCACATG 
GGCCTGCAGT TGCTGACCGT GGCTCTGGAG GCGGCTCACA TCGCCCCCTA CCAGTCACTG 
CTGGGCCTGG TCAAAGACGA GCTCTGCAGA CACCTCTTCC AGTTGCTAAG TGTGGACCGT 
ATGAACCTGT ATGCCGCCTC CGTCCGAGCG TGCTTCTTGC TCTTTGAGAG CATGCGAGGA 
CATTTGAAGT TTCAGCTCGA GATGTATCTG AAGAAGCTGA TGGACATCAT CACATCAGAG 
AACATGAAGA TGCTCTATGA GATGAAGGAG ATGGCCCTGG AGGCCCTGGT GCAGCTCTCG 
AGGATCCCCA GCTTCGTCAC CGAGCTCTAC ATCAATTACG ACTGTGACTT CTACTGCTCA 
AACCTGTTTG AAGACCTCAC CAAGCTGCTC TCTAAGAATG CCTTCCCTGT GTCCGGACAG 
CTCTACACAA CCCACCTCCT CTCACTAGAG GCCCTACTGA CTGTTATTGA CAGCACTGAG 
GCCCACTGCC AGGCCAAGGT GCTCAACAGC AGCGCTCAGC AGGACCAATC AGCAGAAATG 
GTGTTGGCAG AAGGGGAGGG CTCAACCAAC GACCGTACAG ACACAACCAC GGAAGCGAGC 
AGGCAGGTGA ACAGTGCCAA CGGTCAGAAT GTTCCAGAGG CTCACAGTTG GCCCCTACCA 
CCTACCAGCG GGCACCTGAT GGCAGAGAAG ATGAGACTGG GCAGACAAGA CCAGGAGGAG 
ACAGAACCAG CTGAGAAGAA AGAACCAAAA AAGCCACAAC GCTTCTCGTC TTGTTTACCT 
GATTCTCAAG AGCTGTTGGA CATCAGAAAT AAAAAGAAGC TGCTGAACAG CGGTACAGAG 
CAGTTCAACC AGAAGCCTAA GAAGGGCATC CAGTTCCTGC AGGAGAAGGG TCTCCTCAGC 
AGCCCCATGG ACAACAACGA GGTGGCCCAG TGGCTCCGGG AGAACCCCCG CCTCGACAAG 
AAGCAGATCG GAGAGTTCAT CAGTGACCGC AAATACATGG AGTTACTGGA CAGCTTCGTC 
AACACGTTCA CCTTCCGAGG GCTGCGTATT GACGAGGCGC TGCGTCTGTA TCTGGAGGCC 
TTCAGACTGC CAGGAGAGGC TCCGGTCATC CATAGACTTT TGGAAACCTT CACAGACACC 
TGGCATAAAG TGAACGGCTC TCCCTTCATG ACCAATGACG CAGGCTTCGC CCTGGCCTAT 
GCTGTCATCA TGCTAAACAC GGACCAGCAC AACAACAACG TGCGCAAACA GAACATCCCC 
ATGACCATTG AGCAATTCAA GAAGAATCTG AAGGGTGTGA ATGGAAATAC AGACTTTGAT 
CAGGATATGC TGGAAGACAT CTACAATGCA ATCAAGAATG AAGAGATAGT GATGCCAGAC 
GAGCAGTCTG GCCTGGTTAA GGAGAACTAT GTATGGAGCG TGCTGCTCCA CCGGGGGGCC 
ACCTCCGAGG GGGTCTTCCT TCATGTGCCC GCCGGCAGCT ACGACCACGA CCTGTTCACC 
ATGACCTGGG GGCCCACCAT CGCAGCCCTC TCCTACGTCT TCGACAAGAG CCAGGACGAC 
ACAATCATCC AGAAGGCCAT TGCTGGCTTT AGGAAGTGTG CCATGATATC AGCTCATTAT 
GGATTCAGTG ACGTTTTTGA TAATTTGATC ATCTCCCTCT GCAAGTTTAC AATACTCAGC 
AGTGAGTCGG TGGAGAACCT GCCCACAGTA TTTGGGAGTA ACGGGAAGGC ACAGATAGCG 
GCGAAAACGA TGTTCCACCT GGCCCACCGG CACGGTGACA TCCTGAGGGA AGGCTGGAAG 
AACATCATGG ACTCCATGCT GCAGCTGTTC AGAGCCGAGC TGCTGCCCAA GGCCATGCTG 
GAGGTGGAGG ATTTTGTGGA GCCAAATGGA ACGATTTCCC TTCAAAGAGA AGAGACACCG 
TCAAATCGGG GTGAGTCTGC GGTGCTGAGC TTTGTGAACT GGCTGACACT GAGTGGAGCG 
GAGCAGTCTG GTCAACGTGG GCCCTCTACT GAGAACCAGG AGGCCAAGCA GGCAGCCGTG 
CTCTGTATCA AGCAATGTGA CCCAGAGAAG CTGATCACAG AGAGTAAATT CCTGCAGCTG 
GAGTCTCTAC AGGAGCTGAT GAAGGCCCTG ATCTCAGTCA CACCTGATGA GGAGACGTAT 
GACGAGGAGG ATGCTGCGTT CTGCCTGGAG ATGCTGTTAC GCATAGTCAT GGAGAACAGG 
GACCGTGTGT CGTGTGTATG GCAGACGGTG CGGGATCACC TGTGCCACCT GTGTGTCCAG 
GCTACAGAGA GCTGCTTCCT GGTGGAGAGG GCCGTGGTGG GCCTCATAAG ACTGGCCATC 
CGCCTGCTGC GCAGAGAGGA CATCAGCTCC CAGGTGCTGC TGTCCCTGCG TCTCCTGCTG 
ATGATGAAAC CACACGTCCT GGCCCGGGTT AGCCGGGAGG TGGCCTACGG CCTCCATGAG 
CTGCTTAAGA CCAACGCTGC CAACATCCAC TGCACAGACG ACTGGTTCAC CCTTTTCTCC 
CTAATCGAGT GCATCGGGGC CGGGATCAAA CCCCCCGCCT CCTTCAAGGT CACCTCCACT 
ACCCCAGACA TCGACACAGG TGCCCAGTCG GACAGTGAGC TGTCATTCCA CCACCCCAGT 
GAGGTGAGTC TGGACCGGGG GTACACCTCA GACTCTGAGG TCTACACGGA GCACGGAAAG 
TCCAGGATGC CCCGCTCTGT CACTGACGTG GACGTGACCA GCAGCGGATG GCTGGTGGTT 
GGGAAAGACG ACCTGGACAT CAGCAAGGTA GCGGCTTCTG GCTCCAAGAC CCTGCTAAAC 
CCTCTAGTGA ACCAGTACAG CCTGACCCTG GGTCAAGACA TGGGTTTCCA CGACACCAAG 
TCTCTCATCA AGTGTGTGGA GTCCCTCTCC TTCATCGTAC GCGACGCTGC ACACGTCACC 
CCCGAGAACT TTGAGCTGTG TGTCAAGGCC ATCCGGGTGT TTGTGGAGGC CAGCCTAAAC 
GGAGGCTACA GGACCTATGA GAAGAAGAAG AACCACAAGT ACGACTCCCA GAAGTCCCGT 
GTGAGGAGGA AGCCCCGGGA CAAGGAGGGG GCATCCCGCC GCGCCAAGGC CTCCAGCCAG 
AGGCCTTCCC GTTCCCATAG CGATGACGAG GAGGACGAGG GGGTCCCAGC CAGCTACCAC 
ATGGTGTCAT TACAGGTTAG TCAGGACCTG TTAGATCTGA TGCACACGCT GCATACGAGG 
GCCTCCAGCA TCTACAGCTC CTGGGCAGAG GAGCAGCGTC GCCTGGAGCC TGCAGGGAAG 
AAGATTGAGG CCGACTCCCA GACACTGTGG ACCAGCTGCT GGTGCCCACT GCTCCAAGGG 
ATAGCCTGGC TGTGCTGCGA TGCCAGGAGG CTGGTGCGAA TGCAGGCCCT GACCTACCTC 
CAGAGGGCAC TGTTGGTCCA TGACCTGCAG ACACTAGATG CTGTGGAGTG GGAGTCCTGC 
TTCAACAAGG TGCTGTTCCC CCTGCTGACC AAGCTTCTGG ACAATATTAG CCCTGCAGAC 
GTGTGTGGCA TGGAGGAGAC CAGGATGAGG GCTTGCACCC TGCTGTCAAA GGTGTTCCTG 
CAGCACCTCT CTCCCCTGTT GTCTCTGTCC ACCTTCGCTG CCCTGTGGCT CACCATCCTG 
GACTTCATGG ACAAGTACAT GCACGCTGGC TCCAGTGACC TGCTGCTGGA GGCCATCCCA 
GAGTCTCTGA AGAACATGCT GCTGGTGATG GACACAGCAG GAATCTTCCA CAGCGCTGAC 
TCTCGTACGG GATACTCTGA CCTGTGGGAG ATCACCTGGG AACGCATCGA CTGCTTCCTG 
CCTAACCTCC GGGAGGAGCT CTTCAAACAG ACAGTCATAC CACAACCAGA TCCTGTTCCT 
AGTCCCCCAG TGGAGCTTGT CCAGCCGGCT GCCCCCTGCC CCCCAGCCTC GCCACCCCCA 
GCTCAGGCCC CATCTCTTGT GCCAGTGCCA GCTGCCCCCA CAGAGCCAAA GACCTCCAGC 
AGACCAGCGT CGCCCCAAGA TCCCCCACCA CCTAGTACCA ACGGAACAGA CAGATCATCT 
CCAGTACCCC CGTCGACTCC CCCCATGCCC ACCCCAGTGA AGATGTCCCC CCAGATCTCT 
AGCCCACTGA TCCAGTCCCC CACGAGTCAG TCCCCTCTCA TCCTGCAGCC CCTGGCCTCC 
CATCTGCAGG TGGGGGGCGT GCCCCCTATG TCCCTCCCCA TTATTCTCAA CCCCGCCCTC 
ATTGAGGCCA GTTGCCCTGC TCCCCGCCCG CCACCCCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106606442 ( XM_014202673.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014058148.1 Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X4, mRNA.

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CloneID	OAt05305
Clone ID Related Accession (Same CDS sequence)	XM_014202673.1
Accession Version	XM_014202673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012332512.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGAGGATGG TGCGGCTCTC ATCCCCTACT CCCTGCCCTG TCTGCGGGAG 
CTCTTCCGCT TCCTCATCTC ACTGACCAAC CCCCATGACC GCCACAACAC GGACGCCATG 
ATGCACATGG GCCTGCAGTT GCTGACCGTG GCTCTGGAGG CGGCTCACAT CGCCCCCTAC 
CAGTCACTGC TGGGCCTGGT CAAAGACGAG CTCTGCAGAC ACCTCTTCCA GTTGCTAAGT 
GTGGACCGTA TGAACCTGTA TGCCGCCTCC GTCCGAGCGT GCTTCTTGCT CTTTGAGAGC 
ATGCGAGGAC ATTTGAAGTT TCAGCTCGAG ATGTATCTGA AGAAGCTGAT GGACATCATC 
ACATCAGAGA ACATGAAGAT GCTCTATGAG ATGAAGGAGA TGGCCCTGGA GGCCCTGGTG 
CAGCTCTCGA GGATCCCCAG CTTCGTCACC GAGCTCTACA TCAATTACGA CTGTGACTTC 
TACTGCTCAA ACCTGTTTGA AGACCTCACC AAGCTGCTCT CTAAGAATGC CTTCCCTGTG 
TCCGGACAGC TCTACACAAC CCACCTCCTC TCACTAGAGG CCCTACTGAC TGTTATTGAC 
AGCACTGAGG CCCACTGCCA GGCCAAGGTG CTCAACAGCA GCGCTCAGCA GGACCAATCA 
GCAGAAATGG TGTTGGCAGA AGGGGAGGGC TCAACCAACG ACCGTACAGA CACAACCACG 
GAAGCGAGCA GGCAGGTGAA CAGTGCCAAC GGTCAGAATG TTCCAGAGGC TCACAGTTGG 
CCCCTACCAC CTACCAGCGG GCACCTGATG GCAGAGAAGA TGAGACTGGG CAGACAAGAC 
CAGGAGGAGA CAGAACCAGC TGAGAAGAAA GAACCAAAAA AGCCACAACG CTTCTCGTCT 
TGTTTACCTG ATTCTCAAGA GCTGTTGGAC ATCAGAAATA AAAAGAAGCT GCTGAACAGC 
GGTACAGAGC AGTTCAACCA GAAGCCTAAG AAGGGCATCC AGTTCCTGCA GGAGAAGGGT 
CTCCTCAGCA GCCCCATGGA CAACAACGAG GTGGCCCAGT GGCTCCGGGA GAACCCCCGC 
CTCGACAAGA AGCAGATCGG AGAGTTCATC AGTGACCGCA AATACATGGA GTTACTGGAC 
AGCTTCGTCA ACACGTTCAC CTTCCGAGGG CTGCGTATTG ACGAGGCGCT GCGTCTGTAT 
CTGGAGGCCT TCAGACTGCC AGGAGAGGCT CCGGTCATCC ATAGACTTTT GGAAACCTTC 
ACAGACACCT GGCATAAAGT GAACGGCTCT CCCTTCATGA CCAATGACGC AGGCTTCGCC 
CTGGCCTATG CTGTCATCAT GCTAAACACG GACCAGCACA ACAACAACGT GCGCAAACAG 
AACATCCCCA TGACCATTGA GCAATTCAAG AAGAATCTGA AGGGTGTGAA TGGAAATACA 
GACTTTGATC AGGATATGCT GGAAGACATC TACAATGCAA TCAAGAATGA AGAGATAGTG 
ATGCCAGACG AGCAGTCTGG CCTGGTTAAG GAGAACTATG TATGGAGCGT GCTGCTCCAC 
CGGGGGGCCA CCTCCGAGGG GGTCTTCCTT CATGTGCCCG CCGGCAGCTA CGACCACGAC 
CTGTTCACCA TGACCTGGGG GCCCACCATC GCAGCCCTCT CCTACGTCTT CGACAAGAGC 
CAGGACGACA CAATCATCCA GAAGGCCATT GCTGGCTTTA GGAAGTGTGC CATGATATCA 
GCTCATTATG GATTCAGTGA CGTTTTTGAT AATTTGATCA TCTCCCTCTG CAAGTTTACA 
ATACTCAGCA GTGAGTCGGT GGAGAACCTG CCCACAGTAT TTGGGAGTAA CGGGAAGGCA 
CAGATAGCGG CGAAAACGAT GTTCCACCTG GCCCACCGGC ACGGTGACAT CCTGAGGGAA 
GGCTGGAAGA ACATCATGGA CTCCATGCTG CAGCTGTTCA GAGCCGAGCT GCTGCCCAAG 
GCCATGCTGG AGGTGGAGGA TTTTGTGGAG CCAAATGGAA CGATTTCCCT TCAAAGAGAA 
GAGACACCGT CAAATCGGGG TGAGTCTGCG GTGCTGAGCT TTGTGAACTG GCTGACACTG 
AGTGGAGCGG AGCAGTCTGG TCAACGTGGG CCCTCTACTG AGAACCAGGA GGCCAAGCAG 
GCAGCCGTGC TCTGTATCAA GCAATGTGAC CCAGAGAAGC TGATCACAGA GAGTAAATTC 
CTGCAGCTGG AGTCTCTACA GGAGCTGATG AAGGCCCTGA TCTCAGTCAC ACCTGATGAG 
GAGACGTATG ACGAGGAGGA TGCTGCGTTC TGCCTGGAGA TGCTGTTACG CATAGTCATG 
GAGAACAGGG ACCGTGTGTC GTGTGTATGG CAGACGGTGC GGGATCACCT GTGCCACCTG 
TGTGTCCAGG CTACAGAGAG CTGCTTCCTG GTGGAGAGGG CCGTGGTGGG CCTCATAAGA 
CTGGCCATCC GCCTGCTGCG CAGAGAGGAC ATCAGCTCCC AGGTGCTGCT GTCCCTGCGT 
CTCCTGCTGA TGATGAAACC ACACGTCCTG GCCCGGGTTA GCCGGGAGGT GGCCTACGGC 
CTCCATGAGC TGCTTAAGAC CAACGCTGCC AACATCCACT GCACAGACGA CTGGTTCACC 
CTTTTCTCCC TAATCGAGTG CATCGGGGCC GGGATCAAAC CCCCCGCCTC CTTCAAGGTC 
ACCTCCACTA CCCCAGACAT CGACACAGGT GCCCAGTCGG ACAGTGAGCT GTCATTCCAC 
CACCCCAGTG AGGTGAGTCT GGACCGGGGG TACACCTCAG ACTCTGAGGT CTACACGGAG 
CACGGAAAGT CCAGGATGCC CCGCTCTGTC ACTGACGTGG ACGTGACCAG CAGCGGATGG 
CTGGTGGTTG GGAAAGACGA CCTGGACATC AGCAAGGTAG CGGCTTCTGG CTCCAAGACC 
CTGCTAAACC CTCTAGTGAA CCAGTACAGC CTGACCCTGG GTCAAGACAT GGGTTTCCAC 
GACACCAAGT CTCTCATCAA GTGTGTGGAG TCCCTCTCCT TCATCGTACG CGACGCTGCA 
CACGTCACCC CCGAGAACTT TGAGCTGTGT GTCAAGGCCA TCCGGGTGTT TGTGGAGGCC 
AGCCTAAACG GAGGCTACAG GACCTATGAG AAGAAGAAGA ACCACAAGTA CGACTCCCAG 
AAGTCCCGTG TGAGGAGGAA GCCCCGGGAC AAGGAGGGGG CATCCCGCCG CGCCAAGGCC 
TCCAGCCAGA GGCCTTCCCG TTCCCATAGC GATGACGAGG AGGACGAGGG GGTCCCAGCC 
AGCTACCACA TGGTGTCATT ACAGCTGTTA GATCTGATGC ACACGCTGCA TACGAGGGCC 
TCCAGCATCT ACAGCTCCTG GGCAGAGGAG CAGCGTCGCC TGGAGCCTGC AGGGAAGAAG 
ATTGAGGCCG ACTCCCAGAC ACTGTGGACC AGCTGCTGGT GCCCACTGCT CCAAGGGATA 
GCCTGGCTGT GCTGCGATGC CAGGAGGCTG GTGCGAATGC AGGCCCTGAC CTACCTCCAG 
AGGGCACTGT TGGTCCATGA CCTGCAGACA CTAGATGCTG TGGAGTGGGA GTCCTGCTTC 
AACAAGGTGC TGTTCCCCCT GCTGACCAAG CTTCTGGACA ATATTAGCCC TGCAGACGTG 
TGTGGCATGG AGGAGACCAG GATGAGGGCT TGCACCCTGC TGTCAAAGGT GTTCCTGCAG 
CACCTCTCTC CCCTGTTGTC TCTGTCCACC TTCGCTGCCC TGTGGCTCAC CATCCTGGAC 
TTCATGGACA AGTACATGCA CGCTGGCTCC AGTGACCTGC TGCTGGAGGC CATCCCAGAG 
TCTCTGAAGA ACATGCTGCT GGTGATGGAC ACAGCAGGAA TCTTCCACAG CGCTGACTCT 
CGTACGGGAT ACTCTGACCT GTGGGAGATC ACCTGGGAAC GCATCGACTG CTTCCTGCCT 
AACCTCCGGG AGGAGCTCTT CAAACAGACA GTCATACCAC AACCAGATCC TGTTCCTAGT 
CCCCCAGTGG AGCTTGTCCA GCCGGCTGCC CCCTGCCCCC CAGCCTCGCC ACCCCCAGCT 
CAGGCCCCAT CTCTTGTGCC AGTGCCAGCT GCCCCCACAG AGCCAAAGAC CTCCAGCAGA 
CCAGCGTCGC CCCAAGATCC CCCACCACCT AGTACCAACG GAACAGACAG ATCATCTCCA 
GTACCCCCGT CGACTCCCCC CATGCCCACC CCAGTGAAGA TGTCCCCCCA GATCTCTAGC 
CCACTGATCC AGTCCCCCAC GAGTCAGTCC CCTCTCATCC TGCAGCCCCT GGCCTCCCAT 
CTGCAGGTGG GGGGCGTGCC CCCTATGTCC CTCCCCATTA TTCTCAACCC CGCCCTCATT 
GAGGCCAGTT GCCCTGCTCC CCGCCCGCCA CCCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014058148.1
CDS	285..5843
Translation	MVDKNIYIVQGEICTVVGAIKRNSRWNTHTLLDEEQDPLLNSFSHLKEILNNMKELSDVEPNVFLRPFLEVVRSEDTTGPITGLALTSVNKFLSYGLIDANHEAAAEAIENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFSRLPQFKEEAKSFVGANMKKLKMRAGGMSESSKWKKQKRSPHPPRHMVRSASGGLEQAPTTTLSNNLLSGGVPFIEQDSLSVSDSVASSMSIPTDSGLDTCSKATSREDLTDLDQSSSAATTPSTEPGRLYTQSEGRQVEHAQSASVESIPEVLEDGESVTEHSDSASLHDMDYVNPRGVRFTPQQTQRDGEDGAALIPYSLPCLRELFRFLISLTNPHDRHNTDAMMHMGLQLLTVALEAAHIAPYQSLLGLVKDELCRHLFQLLSVDRMNLYAASVRACFLLFESMRGHLKFQLEMYLKKLMDIITSENMKMLYEMKEMALEALVQLSRIPSFVTELYINYDCDFYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLEALLTVIDSTEAHCQAKVLNSSAQQDQSAEMVLAEGEGSTNDRTDTTTEASRQVNSANGQNVPEAHSWPLPPTSGHLMAEKMRLGRQDQEETEPAEKKEPKKPQRFSSCLPDSQELLDIRNKKKLLNSGTEQFNQKPKKGIQFLQEKGLLSSPMDNNEVAQWLRENPRLDKKQIGEFISDRKYMELLDSFVNTFTFRGLRIDEALRLYLEAFRLPGEAPVIHRLLETFTDTWHKVNGSPFMTNDAGFALAYAVIMLNTDQHNNNVRKQNIPMTIEQFKKNLKGVNGNTDFDQDMLEDIYNAIKNEEIVMPDEQSGLVKENYVWSVLLHRGATSEGVFLHVPAGSYDHDLFTMTWGPTIAALSYVFDKSQDDTIIQKAIAGFRKCAMISAHYGFSDVFDNLIISLCKFTILSSESVENLPTVFGSNGKAQIAAKTMFHLAHRHGDILREGWKNIMDSMLQLFRAELLPKAMLEVEDFVEPNGTISLQREETPSNRGESAVLSFVNWLTLSGAEQSGQRGPSTENQEAKQAAVLCIKQCDPEKLITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVMENRDRVSCVWQTVRDHLCHLCVQATESCFLVERAVVGLIRLAIRLLRREDISSQVLLSLRLLLMMKPHVLARVSREVAYGLHELLKTNAANIHCTDDWFTLFSLIECIGAGIKPPASFKVTSTTPDIDTGAQSDSELSFHHPSEVSLDRGYTSDSEVYTEHGKSRMPRSVTDVDVTSSGWLVVGKDDLDISKVAASGSKTLLNPLVNQYSLTLGQDMGFHDTKSLIKCVESLSFIVRDAAHVTPENFELCVKAIRVFVEASLNGGYRTYEKKKNHKYDSQKSRVRRKPRDKEGASRRAKASSQRPSRSHSDDEEDEGVPASYHMVSLQLLDLMHTLHTRASSIYSSWAEEQRRLEPAGKKIEADSQTLWTSCWCPLLQGIAWLCCDARRLVRMQALTYLQRALLVHDLQTLDAVEWESCFNKVLFPLLTKLLDNISPADVCGMEETRMRACTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLLEAIPESLKNMLLVMDTAGIFHSADSRTGYSDLWEITWERIDCFLPNLREELFKQTVIPQPDPVPSPPVELVQPAAPCPPASPPPAQAPSLVPVPAAPTEPKTSSRPASPQDPPPPSTNGTDRSSPVPPSTPPMPTPVKMSPQISSPLIQSPTSQSPLILQPLASHLQVGGVPPMSLPIILNPALIEASCPAPRPPPH

Target ORF information:

RefSeq Version	XM_014202673.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014202673.1

ORF Insert Sequence:

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGAGGATGG TGCGGCTCTC ATCCCCTACT CCCTGCCCTG TCTGCGGGAG 
CTCTTCCGCT TCCTCATCTC ACTGACCAAC CCCCATGACC GCCACAACAC GGACGCCATG 
ATGCACATGG GCCTGCAGTT GCTGACCGTG GCTCTGGAGG CGGCTCACAT CGCCCCCTAC 
CAGTCACTGC TGGGCCTGGT CAAAGACGAG CTCTGCAGAC ACCTCTTCCA GTTGCTAAGT 
GTGGACCGTA TGAACCTGTA TGCCGCCTCC GTCCGAGCGT GCTTCTTGCT CTTTGAGAGC 
ATGCGAGGAC ATTTGAAGTT TCAGCTCGAG ATGTATCTGA AGAAGCTGAT GGACATCATC 
ACATCAGAGA ACATGAAGAT GCTCTATGAG ATGAAGGAGA TGGCCCTGGA GGCCCTGGTG 
CAGCTCTCGA GGATCCCCAG CTTCGTCACC GAGCTCTACA TCAATTACGA CTGTGACTTC 
TACTGCTCAA ACCTGTTTGA AGACCTCACC AAGCTGCTCT CTAAGAATGC CTTCCCTGTG 
TCCGGACAGC TCTACACAAC CCACCTCCTC TCACTAGAGG CCCTACTGAC TGTTATTGAC 
AGCACTGAGG CCCACTGCCA GGCCAAGGTG CTCAACAGCA GCGCTCAGCA GGACCAATCA 
GCAGAAATGG TGTTGGCAGA AGGGGAGGGC TCAACCAACG ACCGTACAGA CACAACCACG 
GAAGCGAGCA GGCAGGTGAA CAGTGCCAAC GGTCAGAATG TTCCAGAGGC TCACAGTTGG 
CCCCTACCAC CTACCAGCGG GCACCTGATG GCAGAGAAGA TGAGACTGGG CAGACAAGAC 
CAGGAGGAGA CAGAACCAGC TGAGAAGAAA GAACCAAAAA AGCCACAACG CTTCTCGTCT 
TGTTTACCTG ATTCTCAAGA GCTGTTGGAC ATCAGAAATA AAAAGAAGCT GCTGAACAGC 
GGTACAGAGC AGTTCAACCA GAAGCCTAAG AAGGGCATCC AGTTCCTGCA GGAGAAGGGT 
CTCCTCAGCA GCCCCATGGA CAACAACGAG GTGGCCCAGT GGCTCCGGGA GAACCCCCGC 
CTCGACAAGA AGCAGATCGG AGAGTTCATC AGTGACCGCA AATACATGGA GTTACTGGAC 
AGCTTCGTCA ACACGTTCAC CTTCCGAGGG CTGCGTATTG ACGAGGCGCT GCGTCTGTAT 
CTGGAGGCCT TCAGACTGCC AGGAGAGGCT CCGGTCATCC ATAGACTTTT GGAAACCTTC 
ACAGACACCT GGCATAAAGT GAACGGCTCT CCCTTCATGA CCAATGACGC AGGCTTCGCC 
CTGGCCTATG CTGTCATCAT GCTAAACACG GACCAGCACA ACAACAACGT GCGCAAACAG 
AACATCCCCA TGACCATTGA GCAATTCAAG AAGAATCTGA AGGGTGTGAA TGGAAATACA 
GACTTTGATC AGGATATGCT GGAAGACATC TACAATGCAA TCAAGAATGA AGAGATAGTG 
ATGCCAGACG AGCAGTCTGG CCTGGTTAAG GAGAACTATG TATGGAGCGT GCTGCTCCAC 
CGGGGGGCCA CCTCCGAGGG GGTCTTCCTT CATGTGCCCG CCGGCAGCTA CGACCACGAC 
CTGTTCACCA TGACCTGGGG GCCCACCATC GCAGCCCTCT CCTACGTCTT CGACAAGAGC 
CAGGACGACA CAATCATCCA GAAGGCCATT GCTGGCTTTA GGAAGTGTGC CATGATATCA 
GCTCATTATG GATTCAGTGA CGTTTTTGAT AATTTGATCA TCTCCCTCTG CAAGTTTACA 
ATACTCAGCA GTGAGTCGGT GGAGAACCTG CCCACAGTAT TTGGGAGTAA CGGGAAGGCA 
CAGATAGCGG CGAAAACGAT GTTCCACCTG GCCCACCGGC ACGGTGACAT CCTGAGGGAA 
GGCTGGAAGA ACATCATGGA CTCCATGCTG CAGCTGTTCA GAGCCGAGCT GCTGCCCAAG 
GCCATGCTGG AGGTGGAGGA TTTTGTGGAG CCAAATGGAA CGATTTCCCT TCAAAGAGAA 
GAGACACCGT CAAATCGGGG TGAGTCTGCG GTGCTGAGCT TTGTGAACTG GCTGACACTG 
AGTGGAGCGG AGCAGTCTGG TCAACGTGGG CCCTCTACTG AGAACCAGGA GGCCAAGCAG 
GCAGCCGTGC TCTGTATCAA GCAATGTGAC CCAGAGAAGC TGATCACAGA GAGTAAATTC 
CTGCAGCTGG AGTCTCTACA GGAGCTGATG AAGGCCCTGA TCTCAGTCAC ACCTGATGAG 
GAGACGTATG ACGAGGAGGA TGCTGCGTTC TGCCTGGAGA TGCTGTTACG CATAGTCATG 
GAGAACAGGG ACCGTGTGTC GTGTGTATGG CAGACGGTGC GGGATCACCT GTGCCACCTG 
TGTGTCCAGG CTACAGAGAG CTGCTTCCTG GTGGAGAGGG CCGTGGTGGG CCTCATAAGA 
CTGGCCATCC GCCTGCTGCG CAGAGAGGAC ATCAGCTCCC AGGTGCTGCT GTCCCTGCGT 
CTCCTGCTGA TGATGAAACC ACACGTCCTG GCCCGGGTTA GCCGGGAGGT GGCCTACGGC 
CTCCATGAGC TGCTTAAGAC CAACGCTGCC AACATCCACT GCACAGACGA CTGGTTCACC 
CTTTTCTCCC TAATCGAGTG CATCGGGGCC GGGATCAAAC CCCCCGCCTC CTTCAAGGTC 
ACCTCCACTA CCCCAGACAT CGACACAGGT GCCCAGTCGG ACAGTGAGCT GTCATTCCAC 
CACCCCAGTG AGGTGAGTCT GGACCGGGGG TACACCTCAG ACTCTGAGGT CTACACGGAG 
CACGGAAAGT CCAGGATGCC CCGCTCTGTC ACTGACGTGG ACGTGACCAG CAGCGGATGG 
CTGGTGGTTG GGAAAGACGA CCTGGACATC AGCAAGGTAG CGGCTTCTGG CTCCAAGACC 
CTGCTAAACC CTCTAGTGAA CCAGTACAGC CTGACCCTGG GTCAAGACAT GGGTTTCCAC 
GACACCAAGT CTCTCATCAA GTGTGTGGAG TCCCTCTCCT TCATCGTACG CGACGCTGCA 
CACGTCACCC CCGAGAACTT TGAGCTGTGT GTCAAGGCCA TCCGGGTGTT TGTGGAGGCC 
AGCCTAAACG GAGGCTACAG GACCTATGAG AAGAAGAAGA ACCACAAGTA CGACTCCCAG 
AAGTCCCGTG TGAGGAGGAA GCCCCGGGAC AAGGAGGGGG CATCCCGCCG CGCCAAGGCC 
TCCAGCCAGA GGCCTTCCCG TTCCCATAGC GATGACGAGG AGGACGAGGG GGTCCCAGCC 
AGCTACCACA TGGTGTCATT ACAGCTGTTA GATCTGATGC ACACGCTGCA TACGAGGGCC 
TCCAGCATCT ACAGCTCCTG GGCAGAGGAG CAGCGTCGCC TGGAGCCTGC AGGGAAGAAG 
ATTGAGGCCG ACTCCCAGAC ACTGTGGACC AGCTGCTGGT GCCCACTGCT CCAAGGGATA 
GCCTGGCTGT GCTGCGATGC CAGGAGGCTG GTGCGAATGC AGGCCCTGAC CTACCTCCAG 
AGGGCACTGT TGGTCCATGA CCTGCAGACA CTAGATGCTG TGGAGTGGGA GTCCTGCTTC 
AACAAGGTGC TGTTCCCCCT GCTGACCAAG CTTCTGGACA ATATTAGCCC TGCAGACGTG 
TGTGGCATGG AGGAGACCAG GATGAGGGCT TGCACCCTGC TGTCAAAGGT GTTCCTGCAG 
CACCTCTCTC CCCTGTTGTC TCTGTCCACC TTCGCTGCCC TGTGGCTCAC CATCCTGGAC 
TTCATGGACA AGTACATGCA CGCTGGCTCC AGTGACCTGC TGCTGGAGGC CATCCCAGAG 
TCTCTGAAGA ACATGCTGCT GGTGATGGAC ACAGCAGGAA TCTTCCACAG CGCTGACTCT 
CGTACGGGAT ACTCTGACCT GTGGGAGATC ACCTGGGAAC GCATCGACTG CTTCCTGCCT 
AACCTCCGGG AGGAGCTCTT CAAACAGACA GTCATACCAC AACCAGATCC TGTTCCTAGT 
CCCCCAGTGG AGCTTGTCCA GCCGGCTGCC CCCTGCCCCC CAGCCTCGCC ACCCCCAGCT 
CAGGCCCCAT CTCTTGTGCC AGTGCCAGCT GCCCCCACAG AGCCAAAGAC CTCCAGCAGA 
CCAGCGTCGC CCCAAGATCC CCCACCACCT AGTACCAACG GAACAGACAG ATCATCTCCA 
GTACCCCCGT CGACTCCCCC CATGCCCACC CCAGTGAAGA TGTCCCCCCA GATCTCTAGC 
CCACTGATCC AGTCCCCCAC GAGTCAGTCC CCTCTCATCC TGCAGCCCCT GGCCTCCCAT 
CTGCAGGTGG GGGGCGTGCC CCCTATGTCC CTCCCCATTA TTCTCAACCC CGCCCTCATT 
GAGGCCAGTT GCCCTGCTCC CCGCCCGCCA CCCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106606442 ( XM_014202680.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014058155.1 Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X5, mRNA.

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CloneID	OAt05306
Clone ID Related Accession (Same CDS sequence)	XM_014202680.1
Accession Version	XM_014202680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012332512.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATATTT GCCTGTGTTC CCTCCCACAG ATCCCATCCT GTATCCTGGA CACACAGGAT 
CTTATTTGTA CAGAGCAGCC TAAAAACACC AATCAGAGGG GCTTAGAGAG GCTGAAGATG 
CGTGCTGGTG GGATGAGTGA GTCGTCAAAG TGGAAGAAGC AGAAGCGCTC GCCCCACCCC 
CCCCGTCACA TGGTGCGAAG TGCCTCTGGC GGGTTGGAGC AGGCCCCTAC CACCACCCTC 
AGCAACAACC TCCTCTCTGG TGGTGTCCCA TTCATCGAGC AGGATAGTTT GTCTGTCTCA 
GACAGCGTGG CCTCCTCCAT GTCCATCCCC ACAGACAGCG GCCTGGACAC CTGCTCCAAG 
GCCACCTCCA GAGAGGACCT GACAGACCTG GACCAGAGCA GCTCTGCTGC CACCACCCCT 
TCCACAGAGC CTGGACGCCT GTACACTCAG TCTGAGGGTA GGCAGGTGGA GCATGCCCAG 
TCAGCCTCGG TGGAGTCCAT CCCTGAGGTC CTGGAGGACG GAGAATCTGT GACGGAGCAT 
TCGGACTCAG CCTCATTACA TGACATGGAC TATGTCAACC CCCGAGGAGT CCGCTTCACA 
CCACAGCAAA CACAGAGAGA CGGTGAGGAT GGTGCGGCTC TCATCCCCTA CTCCCTGCCC 
TGTCTGCGGG AGCTCTTCCG CTTCCTCATC TCACTGACCA ACCCCCATGA CCGCCACAAC 
ACGGACGCCA TGATGCACAT GGGCCTGCAG TTGCTGACCG TGGCTCTGGA GGCGGCTCAC 
ATCGCCCCCT ACCAGTCACT GCTGGGCCTG GTCAAAGACG AGCTCTGCAG ACACCTCTTC 
CAGTTGCTAA GTGTGGACCG TATGAACCTG TATGCCGCCT CCGTCCGAGC GTGCTTCTTG 
CTCTTTGAGA GCATGCGAGG ACATTTGAAG TTTCAGCTCG AGATGTATCT GAAGAAGCTG 
ATGGACATCA TCACATCAGA GAACATGAAG ATGCTCTATG AGATGAAGGA GATGGCCCTG 
GAGGCCCTGG TGCAGCTCTC GAGGATCCCC AGCTTCGTCA CCGAGCTCTA CATCAATTAC 
GACTGTGACT TCTACTGCTC AAACCTGTTT GAAGACCTCA CCAAGCTGCT CTCTAAGAAT 
GCCTTCCCTG TGTCCGGACA GCTCTACACA ACCCACCTCC TCTCACTAGA GGCCCTACTG 
ACTGTTATTG ACAGCACTGA GGCCCACTGC CAGGCCAAGG TGCTCAACAG CAGCGCTCAG 
CAGGACCAAT CAGCAGAAAT GGTGTTGGCA GAAGGGGAGG GCTCAACCAA CGACCGTACA 
GACACAACCA CGGAAGCGAG CAGGCAGGTG AACAGTGCCA ACGGTCAGAA TGTTCCAGAG 
GCTCACAGTT GGCCCCTACC ACCTACCAGC GGGCACCTGA TGGCAGAGAA GATGAGACTG 
GGCAGACAAG ACCAGGAGGA GACAGAACCA GCTGAGAAGA AAGAACCAAA AAAGCCACAA 
CGCTTCTCGT CTTGTTTACC TGATTCTCAA GAGCTGTTGG ACATCAGAAA TAAAAAGAAG 
CTGCTGAACA GCGGTACAGA GCAGTTCAAC CAGAAGCCTA AGAAGGGCAT CCAGTTCCTG 
CAGGAGAAGG GTCTCCTCAG CAGCCCCATG GACAACAACG AGGTGGCCCA GTGGCTCCGG 
GAGAACCCCC GCCTCGACAA GAAGCAGATC GGAGAGTTCA TCAGTGACCG CAAATACATG 
GAGTTACTGG ACAGCTTCGT CAACACGTTC ACCTTCCGAG GGCTGCGTAT TGACGAGGCG 
CTGCGTCTGT ATCTGGAGGC CTTCAGACTG CCAGGAGAGG CTCCGGTCAT CCATAGACTT 
TTGGAAACCT TCACAGACAC CTGGCATAAA GTGAACGGCT CTCCCTTCAT GACCAATGAC 
GCAGGCTTCG CCCTGGCCTA TGCTGTCATC ATGCTAAACA CGGACCAGCA CAACAACAAC 
GTGCGCAAAC AGAACATCCC CATGACCATT GAGCAATTCA AGAAGAATCT GAAGGGTGTG 
AATGGAAATA CAGACTTTGA TCAGGATATG CTGGAAGACA TCTACAATGC AATCAAGAAT 
GAAGAGATAG TGATGCCAGA CGAGCAGTCT GGCCTGGTTA AGGAGAACTA TGTATGGAGC 
GTGCTGCTCC ACCGGGGGGC CACCTCCGAG GGGGTCTTCC TTCATGTGCC CGCCGGCAGC 
TACGACCACG ACCTGTTCAC CATGACCTGG GGGCCCACCA TCGCAGCCCT CTCCTACGTC 
TTCGACAAGA GCCAGGACGA CACAATCATC CAGAAGGCCA TTGCTGGCTT TAGGAAGTGT 
GCCATGATAT CAGCTCATTA TGGATTCAGT GACGTTTTTG ATAATTTGAT CATCTCCCTC 
TGCAAGTTTA CAATACTCAG CAGTGAGTCG GTGGAGAACC TGCCCACAGT ATTTGGGAGT 
AACGGGAAGG CACAGATAGC GGCGAAAACG ATGTTCCACC TGGCCCACCG GCACGGTGAC 
ATCCTGAGGG AAGGCTGGAA GAACATCATG GACTCCATGC TGCAGCTGTT CAGAGCCGAG 
CTGCTGCCCA AGGCCATGCT GGAGGTGGAG GATTTTGTGG AGCCAAATGG AACGATTTCC 
CTTCAAAGAG AAGAGACACC GTCAAATCGG GGTGAGTCTG CGGTGCTGAG CTTTGTGAAC 
TGGCTGACAC TGAGTGGAGC GGAGCAGTCT GGTCAACGTG GGCCCTCTAC TGAGAACCAG 
GAGGCCAAGC AGGCAGCCGT GCTCTGTATC AAGCAATGTG ACCCAGAGAA GCTGATCACA 
GAGAGTAAAT TCCTGCAGCT GGAGTCTCTA CAGGAGCTGA TGAAGGCCCT GATCTCAGTC 
ACACCTGATG AGGAGACGTA TGACGAGGAG GATGCTGCGT TCTGCCTGGA GATGCTGTTA 
CGCATAGTCA TGGAGAACAG GGACCGTGTG TCGTGTGTAT GGCAGACGGT GCGGGATCAC 
CTGTGCCACC TGTGTGTCCA GGCTACAGAG AGCTGCTTCC TGGTGGAGAG GGCCGTGGTG 
GGCCTCATAA GACTGGCCAT CCGCCTGCTG CGCAGAGAGG ACATCAGCTC CCAGGTGCTG 
CTGTCCCTGC GTCTCCTGCT GATGATGAAA CCACACGTCC TGGCCCGGGT TAGCCGGGAG 
GTGGCCTACG GCCTCCATGA GCTGCTTAAG ACCAACGCTG CCAACATCCA CTGCACAGAC 
GACTGGTTCA CCCTTTTCTC CCTAATCGAG TGCATCGGGG CCGGGATCAA ACCCCCCGCC 
TCCTTCAAGG TCACCTCCAC TACCCCAGAC ATCGACACAG GTGCCCAGTC GGACAGTGAG 
CTGTCATTCC ACCACCCCAG TGAGGTGAGT CTGGACCGGG GGTACACCTC AGACTCTGAG 
GTCTACACGG AGCACGGAAA GTCCAGGATG CCCCGCTCTG TCACTGACGT GGACGTGACC 
AGCAGCGGAT GGCTGGTGGT TGGGAAAGAC GACCTGGACA TCAGCAAGGT AGCGGCTTCT 
GGCTCCAAGA CCCTGCTAAA CCCTCTAGTG AACCAGTACA GCCTGACCCT GGGTCAAGAC 
ATGGGTTTCC ACGACACCAA GTCTCTCATC AAGTGTGTGG AGTCCCTCTC CTTCATCGTA 
CGCGACGCTG CACACGTCAC CCCCGAGAAC TTTGAGCTGT GTGTCAAGGC CATCCGGGTG 
TTTGTGGAGG CCAGCCTAAA CGGAGGCTAC AGGACCTATG AGAAGAAGAA GAACCACAAG 
TACGACTCCC AGAAGTCCCG TGTGAGGAGG AAGCCCCGGG ACAAGGAGGG GGCATCCCGC 
CGCGCCAAGG CCTCCAGCCA GAGGCCTTCC CGTTCCCATA GCGATGACGA GGAGGACGAG 
GGGGTCCCAG CCAGCTACCA CATGGTGTCA TTACAGGTTA GTCAGGACCT GTTAGATCTG 
ATGCACACGC TGCATACGAG GGCCTCCAGC ATCTACAGCT CCTGGGCAGA GGAGCAGCGT 
CGCCTGGAGC CTGCAGGGAA GAAGATTGAG GCCGACTCCC AGACACTGTG GACCAGCTGC 
TGGTGCCCAC TGCTCCAAGG GATAGCCTGG CTGTGCTGCG ATGCCAGGAG GCTGGTGCGA 
ATGCAGGCCC TGACCTACCT CCAGAGGGCA CTGTTGGTCC ATGACCTGCA GACACTAGAT 
GCTGTGGAGT GGGAGTCCTG CTTCAACAAG GTGCTGTTCC CCCTGCTGAC CAAGCTTCTG 
GACAATATTA GCCCTGCAGA CGTGTGTGGC ATGGAGGAGA CCAGGATGAG GGCTTGCACC 
CTGCTGTCAA AGGTGTTCCT GCAGCACCTC TCTCCCCTGT TGTCTCTGTC CACCTTCGCT 
GCCCTGTGGC TCACCATCCT GGACTTCATG GACAAGTACA TGCACGCTGG CTCCAGTGAC 
CTGCTGCTGG AGGCCATCCC AGAGTCTCTG AAGAACATGC TGCTGGTGAT GGACACAGCA 
GGAATCTTCC ACAGCGCTGA CTCTCGTACG GGATACTCTG ACCTGTGGGA GATCACCTGG 
GAACGCATCG ACTGCTTCCT GCCTAACCTC CGGGAGGAGC TCTTCAAACA GACAGTCATA 
CCACAACCAG ATCCTGTTCC TAGTCCCCCA GTGGAGCTTG TCCAGCCGGC TGCCCCCTGC 
CCCCCAGCCT CGCCACCCCC AGCTCAGGCC CCATCTCTTG TGCCAGTGCC AGCTGCCCCC 
ACAGAGCCAA AGACCTCCAG CAGACCAGCG TCGCCCCAAG ATCCCCCACC ACCTAGTACC 
AACGGAACAG ACAGATCATC TCCAGTACCC CCGTCGACTC CCCCCATGCC CACCCCAGTG 
AAGATGTCCC CCCAGATCTC TAGCCCACTG ATCCAGTCCC CCACGAGTCA GTCCCCTCTC 
ATCCTGCAGC CCCTGGCCTC CCATCTGCAG GTGGGGGGCG TGCCCCCTAT GTCCCTCCCC 
ATTATTCTCA ACCCCGCCCT CATTGAGGCC AGTTGCCCTG CTCCCCGCCC GCCACCCCAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014058155.1
CDS	16..5058
Translation	MHICLCSLPQIPSCILDTQDLICTEQPKNTNQRGLERLKMRAGGMSESSKWKKQKRSPHPPRHMVRSASGGLEQAPTTTLSNNLLSGGVPFIEQDSLSVSDSVASSMSIPTDSGLDTCSKATSREDLTDLDQSSSAATTPSTEPGRLYTQSEGRQVEHAQSASVESIPEVLEDGESVTEHSDSASLHDMDYVNPRGVRFTPQQTQRDGEDGAALIPYSLPCLRELFRFLISLTNPHDRHNTDAMMHMGLQLLTVALEAAHIAPYQSLLGLVKDELCRHLFQLLSVDRMNLYAASVRACFLLFESMRGHLKFQLEMYLKKLMDIITSENMKMLYEMKEMALEALVQLSRIPSFVTELYINYDCDFYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLEALLTVIDSTEAHCQAKVLNSSAQQDQSAEMVLAEGEGSTNDRTDTTTEASRQVNSANGQNVPEAHSWPLPPTSGHLMAEKMRLGRQDQEETEPAEKKEPKKPQRFSSCLPDSQELLDIRNKKKLLNSGTEQFNQKPKKGIQFLQEKGLLSSPMDNNEVAQWLRENPRLDKKQIGEFISDRKYMELLDSFVNTFTFRGLRIDEALRLYLEAFRLPGEAPVIHRLLETFTDTWHKVNGSPFMTNDAGFALAYAVIMLNTDQHNNNVRKQNIPMTIEQFKKNLKGVNGNTDFDQDMLEDIYNAIKNEEIVMPDEQSGLVKENYVWSVLLHRGATSEGVFLHVPAGSYDHDLFTMTWGPTIAALSYVFDKSQDDTIIQKAIAGFRKCAMISAHYGFSDVFDNLIISLCKFTILSSESVENLPTVFGSNGKAQIAAKTMFHLAHRHGDILREGWKNIMDSMLQLFRAELLPKAMLEVEDFVEPNGTISLQREETPSNRGESAVLSFVNWLTLSGAEQSGQRGPSTENQEAKQAAVLCIKQCDPEKLITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVMENRDRVSCVWQTVRDHLCHLCVQATESCFLVERAVVGLIRLAIRLLRREDISSQVLLSLRLLLMMKPHVLARVSREVAYGLHELLKTNAANIHCTDDWFTLFSLIECIGAGIKPPASFKVTSTTPDIDTGAQSDSELSFHHPSEVSLDRGYTSDSEVYTEHGKSRMPRSVTDVDVTSSGWLVVGKDDLDISKVAASGSKTLLNPLVNQYSLTLGQDMGFHDTKSLIKCVESLSFIVRDAAHVTPENFELCVKAIRVFVEASLNGGYRTYEKKKNHKYDSQKSRVRRKPRDKEGASRRAKASSQRPSRSHSDDEEDEGVPASYHMVSLQVSQDLLDLMHTLHTRASSIYSSWAEEQRRLEPAGKKIEADSQTLWTSCWCPLLQGIAWLCCDARRLVRMQALTYLQRALLVHDLQTLDAVEWESCFNKVLFPLLTKLLDNISPADVCGMEETRMRACTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLLEAIPESLKNMLLVMDTAGIFHSADSRTGYSDLWEITWERIDCFLPNLREELFKQTVIPQPDPVPSPPVELVQPAAPCPPASPPPAQAPSLVPVPAAPTEPKTSSRPASPQDPPPPSTNGTDRSSPVPPSTPPMPTPVKMSPQISSPLIQSPTSQSPLILQPLASHLQVGGVPPMSLPIILNPALIEASCPAPRPPPH

Target ORF information:

RefSeq Version	XM_014202680.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014202680.1

ORF Insert Sequence:

ATGCATATTT GCCTGTGTTC CCTCCCACAG ATCCCATCCT GTATCCTGGA CACACAGGAT 
CTTATTTGTA CAGAGCAGCC TAAAAACACC AATCAGAGGG GCTTAGAGAG GCTGAAGATG 
CGTGCTGGTG GGATGAGTGA GTCGTCAAAG TGGAAGAAGC AGAAGCGCTC GCCCCACCCC 
CCCCGTCACA TGGTGCGAAG TGCCTCTGGC GGGTTGGAGC AGGCCCCTAC CACCACCCTC 
AGCAACAACC TCCTCTCTGG TGGTGTCCCA TTCATCGAGC AGGATAGTTT GTCTGTCTCA 
GACAGCGTGG CCTCCTCCAT GTCCATCCCC ACAGACAGCG GCCTGGACAC CTGCTCCAAG 
GCCACCTCCA GAGAGGACCT GACAGACCTG GACCAGAGCA GCTCTGCTGC CACCACCCCT 
TCCACAGAGC CTGGACGCCT GTACACTCAG TCTGAGGGTA GGCAGGTGGA GCATGCCCAG 
TCAGCCTCGG TGGAGTCCAT CCCTGAGGTC CTGGAGGACG GAGAATCTGT GACGGAGCAT 
TCGGACTCAG CCTCATTACA TGACATGGAC TATGTCAACC CCCGAGGAGT CCGCTTCACA 
CCACAGCAAA CACAGAGAGA CGGTGAGGAT GGTGCGGCTC TCATCCCCTA CTCCCTGCCC 
TGTCTGCGGG AGCTCTTCCG CTTCCTCATC TCACTGACCA ACCCCCATGA CCGCCACAAC 
ACGGACGCCA TGATGCACAT GGGCCTGCAG TTGCTGACCG TGGCTCTGGA GGCGGCTCAC 
ATCGCCCCCT ACCAGTCACT GCTGGGCCTG GTCAAAGACG AGCTCTGCAG ACACCTCTTC 
CAGTTGCTAA GTGTGGACCG TATGAACCTG TATGCCGCCT CCGTCCGAGC GTGCTTCTTG 
CTCTTTGAGA GCATGCGAGG ACATTTGAAG TTTCAGCTCG AGATGTATCT GAAGAAGCTG 
ATGGACATCA TCACATCAGA GAACATGAAG ATGCTCTATG AGATGAAGGA GATGGCCCTG 
GAGGCCCTGG TGCAGCTCTC GAGGATCCCC AGCTTCGTCA CCGAGCTCTA CATCAATTAC 
GACTGTGACT TCTACTGCTC AAACCTGTTT GAAGACCTCA CCAAGCTGCT CTCTAAGAAT 
GCCTTCCCTG TGTCCGGACA GCTCTACACA ACCCACCTCC TCTCACTAGA GGCCCTACTG 
ACTGTTATTG ACAGCACTGA GGCCCACTGC CAGGCCAAGG TGCTCAACAG CAGCGCTCAG 
CAGGACCAAT CAGCAGAAAT GGTGTTGGCA GAAGGGGAGG GCTCAACCAA CGACCGTACA 
GACACAACCA CGGAAGCGAG CAGGCAGGTG AACAGTGCCA ACGGTCAGAA TGTTCCAGAG 
GCTCACAGTT GGCCCCTACC ACCTACCAGC GGGCACCTGA TGGCAGAGAA GATGAGACTG 
GGCAGACAAG ACCAGGAGGA GACAGAACCA GCTGAGAAGA AAGAACCAAA AAAGCCACAA 
CGCTTCTCGT CTTGTTTACC TGATTCTCAA GAGCTGTTGG ACATCAGAAA TAAAAAGAAG 
CTGCTGAACA GCGGTACAGA GCAGTTCAAC CAGAAGCCTA AGAAGGGCAT CCAGTTCCTG 
CAGGAGAAGG GTCTCCTCAG CAGCCCCATG GACAACAACG AGGTGGCCCA GTGGCTCCGG 
GAGAACCCCC GCCTCGACAA GAAGCAGATC GGAGAGTTCA TCAGTGACCG CAAATACATG 
GAGTTACTGG ACAGCTTCGT CAACACGTTC ACCTTCCGAG GGCTGCGTAT TGACGAGGCG 
CTGCGTCTGT ATCTGGAGGC CTTCAGACTG CCAGGAGAGG CTCCGGTCAT CCATAGACTT 
TTGGAAACCT TCACAGACAC CTGGCATAAA GTGAACGGCT CTCCCTTCAT GACCAATGAC 
GCAGGCTTCG CCCTGGCCTA TGCTGTCATC ATGCTAAACA CGGACCAGCA CAACAACAAC 
GTGCGCAAAC AGAACATCCC CATGACCATT GAGCAATTCA AGAAGAATCT GAAGGGTGTG 
AATGGAAATA CAGACTTTGA TCAGGATATG CTGGAAGACA TCTACAATGC AATCAAGAAT 
GAAGAGATAG TGATGCCAGA CGAGCAGTCT GGCCTGGTTA AGGAGAACTA TGTATGGAGC 
GTGCTGCTCC ACCGGGGGGC CACCTCCGAG GGGGTCTTCC TTCATGTGCC CGCCGGCAGC 
TACGACCACG ACCTGTTCAC CATGACCTGG GGGCCCACCA TCGCAGCCCT CTCCTACGTC 
TTCGACAAGA GCCAGGACGA CACAATCATC CAGAAGGCCA TTGCTGGCTT TAGGAAGTGT 
GCCATGATAT CAGCTCATTA TGGATTCAGT GACGTTTTTG ATAATTTGAT CATCTCCCTC 
TGCAAGTTTA CAATACTCAG CAGTGAGTCG GTGGAGAACC TGCCCACAGT ATTTGGGAGT 
AACGGGAAGG CACAGATAGC GGCGAAAACG ATGTTCCACC TGGCCCACCG GCACGGTGAC 
ATCCTGAGGG AAGGCTGGAA GAACATCATG GACTCCATGC TGCAGCTGTT CAGAGCCGAG 
CTGCTGCCCA AGGCCATGCT GGAGGTGGAG GATTTTGTGG AGCCAAATGG AACGATTTCC 
CTTCAAAGAG AAGAGACACC GTCAAATCGG GGTGAGTCTG CGGTGCTGAG CTTTGTGAAC 
TGGCTGACAC TGAGTGGAGC GGAGCAGTCT GGTCAACGTG GGCCCTCTAC TGAGAACCAG 
GAGGCCAAGC AGGCAGCCGT GCTCTGTATC AAGCAATGTG ACCCAGAGAA GCTGATCACA 
GAGAGTAAAT TCCTGCAGCT GGAGTCTCTA CAGGAGCTGA TGAAGGCCCT GATCTCAGTC 
ACACCTGATG AGGAGACGTA TGACGAGGAG GATGCTGCGT TCTGCCTGGA GATGCTGTTA 
CGCATAGTCA TGGAGAACAG GGACCGTGTG TCGTGTGTAT GGCAGACGGT GCGGGATCAC 
CTGTGCCACC TGTGTGTCCA GGCTACAGAG AGCTGCTTCC TGGTGGAGAG GGCCGTGGTG 
GGCCTCATAA GACTGGCCAT CCGCCTGCTG CGCAGAGAGG ACATCAGCTC CCAGGTGCTG 
CTGTCCCTGC GTCTCCTGCT GATGATGAAA CCACACGTCC TGGCCCGGGT TAGCCGGGAG 
GTGGCCTACG GCCTCCATGA GCTGCTTAAG ACCAACGCTG CCAACATCCA CTGCACAGAC 
GACTGGTTCA CCCTTTTCTC CCTAATCGAG TGCATCGGGG CCGGGATCAA ACCCCCCGCC 
TCCTTCAAGG TCACCTCCAC TACCCCAGAC ATCGACACAG GTGCCCAGTC GGACAGTGAG 
CTGTCATTCC ACCACCCCAG TGAGGTGAGT CTGGACCGGG GGTACACCTC AGACTCTGAG 
GTCTACACGG AGCACGGAAA GTCCAGGATG CCCCGCTCTG TCACTGACGT GGACGTGACC 
AGCAGCGGAT GGCTGGTGGT TGGGAAAGAC GACCTGGACA TCAGCAAGGT AGCGGCTTCT 
GGCTCCAAGA CCCTGCTAAA CCCTCTAGTG AACCAGTACA GCCTGACCCT GGGTCAAGAC 
ATGGGTTTCC ACGACACCAA GTCTCTCATC AAGTGTGTGG AGTCCCTCTC CTTCATCGTA 
CGCGACGCTG CACACGTCAC CCCCGAGAAC TTTGAGCTGT GTGTCAAGGC CATCCGGGTG 
TTTGTGGAGG CCAGCCTAAA CGGAGGCTAC AGGACCTATG AGAAGAAGAA GAACCACAAG 
TACGACTCCC AGAAGTCCCG TGTGAGGAGG AAGCCCCGGG ACAAGGAGGG GGCATCCCGC 
CGCGCCAAGG CCTCCAGCCA GAGGCCTTCC CGTTCCCATA GCGATGACGA GGAGGACGAG 
GGGGTCCCAG CCAGCTACCA CATGGTGTCA TTACAGGTTA GTCAGGACCT GTTAGATCTG 
ATGCACACGC TGCATACGAG GGCCTCCAGC ATCTACAGCT CCTGGGCAGA GGAGCAGCGT 
CGCCTGGAGC CTGCAGGGAA GAAGATTGAG GCCGACTCCC AGACACTGTG GACCAGCTGC 
TGGTGCCCAC TGCTCCAAGG GATAGCCTGG CTGTGCTGCG ATGCCAGGAG GCTGGTGCGA 
ATGCAGGCCC TGACCTACCT CCAGAGGGCA CTGTTGGTCC ATGACCTGCA GACACTAGAT 
GCTGTGGAGT GGGAGTCCTG CTTCAACAAG GTGCTGTTCC CCCTGCTGAC CAAGCTTCTG 
GACAATATTA GCCCTGCAGA CGTGTGTGGC ATGGAGGAGA CCAGGATGAG GGCTTGCACC 
CTGCTGTCAA AGGTGTTCCT GCAGCACCTC TCTCCCCTGT TGTCTCTGTC CACCTTCGCT 
GCCCTGTGGC TCACCATCCT GGACTTCATG GACAAGTACA TGCACGCTGG CTCCAGTGAC 
CTGCTGCTGG AGGCCATCCC AGAGTCTCTG AAGAACATGC TGCTGGTGAT GGACACAGCA 
GGAATCTTCC ACAGCGCTGA CTCTCGTACG GGATACTCTG ACCTGTGGGA GATCACCTGG 
GAACGCATCG ACTGCTTCCT GCCTAACCTC CGGGAGGAGC TCTTCAAACA GACAGTCATA 
CCACAACCAG ATCCTGTTCC TAGTCCCCCA GTGGAGCTTG TCCAGCCGGC TGCCCCCTGC 
CCCCCAGCCT CGCCACCCCC AGCTCAGGCC CCATCTCTTG TGCCAGTGCC AGCTGCCCCC 
ACAGAGCCAA AGACCTCCAG CAGACCAGCG TCGCCCCAAG ATCCCCCACC ACCTAGTACC 
AACGGAACAG ACAGATCATC TCCAGTACCC CCGTCGACTC CCCCCATGCC CACCCCAGTG 
AAGATGTCCC CCCAGATCTC TAGCCCACTG ATCCAGTCCC CCACGAGTCA GTCCCCTCTC 
ATCCTGCAGC CCCTGGCCTC CCATCTGCAG GTGGGGGGCG TGCCCCCTAT GTCCCTCCCC 
ATTATTCTCA ACCCCGCCCT CATTGAGGCC AGTTGCCCTG CTCCCCGCCC GCCACCCCAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106606442 ( XM_014202687.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014058162.1 Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X6, mRNA.

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CloneID	OAt05307
Clone ID Related Accession (Same CDS sequence)	XM_014202687.1
Accession Version	XM_014202687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012332512.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGCGGCTCT CATCCCCTAC TCCCTGCCCT GTCTGCGGGA GCTCTTCCGC 
TTCCTCATCT CACTGACCAA CCCCCATGAC CGCCACAACA CGGACGCCAT GATGCACATG 
GGCCTGCAGT TGCTGACCGT GGCTCTGGAG GCGGCTCACA TCGCCCCCTA CCAGTCACTG 
CTGGGCCTGG TCAAAGACGA GCTCTGCAGA CACCTCTTCC AGTTGCTAAG TGTGGACCGT 
ATGAACCTGT ATGCCGCCTC CGTCCGAGCG TGCTTCTTGC TCTTTGAGAG CATGCGAGGA 
CATTTGAAGT TTCAGCTCGA GATGTATCTG AAGAAGCTGA TGGACATCAT CACATCAGAG 
AACATGAAGA TGCTCTATGA GATGAAGGAG ATGGCCCTGG AGGCCCTGGT GCAGCTCTCG 
AGGATCCCCA GCTTCGTCAC CGAGCTCTAC ATCAATTACG ACTGTGACTT CTACTGCTCA 
AACCTGTTTG AAGACCTCAC CAAGCTGCTC TCTAAGAATG CCTTCCCTGT GTCCGGACAG 
CTCTACACAA CCCACCTCCT CTCACTAGAG GCCCTACTGA CTGTTATTGA CAGCACTGAG 
GCCCACTGCC AGGCCAAGGT GCTCAACAGC AGCGCTCAGC AGGACCAATC AGCAGAAATG 
GTGTTGGCAG AAGGGGAGGG CTCAACCAAC GACCGTACAG ACACAACCAC GGAAGCGAGC 
AGGCAGGTGA ACAGTGCCAA CGGTCAGAAT GTTCCAGAGG CTCACAGTTG GCCCCTACCA 
CCTACCAGCG GGCACCTGAT GGCAGAGAAG ATGAGACTGG GCAGACAAGA CCAGGAGGAG 
ACAGAACCAG CTGAGAAGAA AGAACCAAAA AAGCCACAAC GCTTCTCGTC TTGTTTACCT 
GATTCTCAAG AGCTGTTGGA CATCAGAAAT AAAAAGAAGC TGCTGAACAG CGGTACAGAG 
CAGTTCAACC AGAAGCCTAA GAAGGGCATC CAGTTCCTGC AGGAGAAGGG TCTCCTCAGC 
AGCCCCATGG ACAACAACGA GGTGGCCCAG TGGCTCCGGG AGAACCCCCG CCTCGACAAG 
AAGCAGATCG GAGAGTTCAT CAGTGACCGC AAATACATGG AGTTACTGGA CAGCTTCGTC 
AACACGTTCA CCTTCCGAGG GCTGCGTATT GACGAGGCGC TGCGTCTGTA TCTGGAGGCC 
TTCAGACTGC CAGGAGAGGC TCCGGTCATC CATAGACTTT TGGAAACCTT CACAGACACC 
TGGCATAAAG TGAACGGCTC TCCCTTCATG ACCAATGACG CAGGCTTCGC CCTGGCCTAT 
GCTGTCATCA TGCTAAACAC GGACCAGCAC AACAACAACG TGCGCAAACA GAACATCCCC 
ATGACCATTG AGCAATTCAA GAAGAATCTG AAGGGTGTGA ATGGAAATAC AGACTTTGAT 
CAGGATATGC TGGAAGACAT CTACAATGCA ATCAAGAATG AAGAGATAGT GATGCCAGAC 
GAGCAGTCTG GCCTGGTTAA GGAGAACTAT GTATGGAGCG TGCTGCTCCA CCGGGGGGCC 
ACCTCCGAGG GGGTCTTCCT TCATGTGCCC GCCGGCAGCT ACGACCACGA CCTGTTCACC 
ATGACCTGGG GGCCCACCAT CGCAGCCCTC TCCTACGTCT TCGACAAGAG CCAGGACGAC 
ACAATCATCC AGAAGGCCAT TGCTGGCTTT AGGAAGTGTG CCATGATATC AGCTCATTAT 
GGATTCAGTG ACGTTTTTGA TAATTTGATC ATCTCCCTCT GCAAGTTTAC AATACTCAGC 
AGTGAGTCGG TGGAGAACCT GCCCACAGTA TTTGGGAGTA ACGGGAAGGC ACAGATAGCG 
GCGAAAACGA TGTTCCACCT GGCCCACCGG CACGGTGACA TCCTGAGGGA AGGCTGGAAG 
AACATCATGG ACTCCATGCT GCAGCTGTTC AGAGCCGAGC TGCTGCCCAA GGCCATGCTG 
GAGGTGGAGG ATTTTGTGGA GCCAAATGGA ACGATTTCCC TTCAAAGAGA AGAGACACCG 
TCAAATCGGG GTGAGTCTGC GGTGCTGAGC TTTGTGAACT GGCTGACACT GAGTGGAGCG 
GAGCAGTCTG GTCAACGTGG GCCCTCTACT GAGAACCAGG AGGCCAAGCA GGCAGCCGTG 
CTCTGTATCA AGCAATGTGA CCCAGAGAAG CTGATCACAG AGAGTAAATT CCTGCAGCTG 
GAGTCTCTAC AGGAGCTGAT GAAGGCCCTG ATCTCAGTCA CACCTGATGA GGAGACGTAT 
GACGAGGAGG ATGCTGCGTT CTGCCTGGAG ATGCTGTTAC GCATAGTCAT GGAGAACAGG 
GACCGTGTGT CGTGTGTATG GCAGACGGTG CGGGATCACC TGTGCCACCT GTGTGTCCAG 
GCTACAGAGA GCTGCTTCCT GGTGGAGAGG GCCGTGGTGG GCCTCATAAG ACTGGCCATC 
CGCCTGCTGC GCAGAGAGGA CATCAGCTCC CAGGTGCTGC TGTCCCTGCG TCTCCTGCTG 
ATGATGAAAC CACACGTCCT GGCCCGGGTT AGCCGGGAGG TGGCCTACGG CCTCCATGAG 
CTGCTTAAGA CCAACGCTGC CAACATCCAC TGCACAGACG ACTGGTTCAC CCTTTTCTCC 
CTAATCGAGT GCATCGGGGC CGGGATCAAA CCCCCCGCCT CCTTCAAGGT CACCTCCACT 
ACCCCAGACA TCGACACAGG TGCCCAGTCG GACAGTGAGC TGTCATTCCA CCACCCCAGT 
GAGGTGAGTC TGGACCGGGG GTACACCTCA GACTCTGAGG TCTACACGGA GCACGGAAAG 
TCCAGGATGC CCCGCTCTGT CACTGACGTG GACGTGACCA GCAGCGGATG GCTGGTGGTT 
GGGAAAGACG ACCTGGACAT CAGCAAGGTA GCGGCTTCTG GCTCCAAGAC CCTGCTAAAC 
CCTCTAGTGA ACCAGTACAG CCTGACCCTG GGTCAAGACA TGGGTTTCCA CGACACCAAG 
TCTCTCATCA AGTGTGTGGA GTCCCTCTCC TTCATCGTAC GCGACGCTGC ACACGTCACC 
CCCGAGAACT TTGAGCTGTG TGTCAAGGCC ATCCGGGTGT TTGTGGAGGC CAGCCTAAAC 
GGAGGCTACA GGACCTATGA GAAGAAGAAG AACCACAAGT ACGACTCCCA GAAGTCCCGT 
GTGAGGAGGA AGCCCCGGGA CAAGGAGGGG GCATCCCGCC GCGCCAAGGC CTCCAGCCAG 
AGGCCTTCCC GTTCCCATAG CGATGACGAG GAGGACGAGG GGGTCCCAGC CAGCTACCAC 
ATGGTGTCAT TACAGCTGTT AGATCTGATG CACACGCTGC ATACGAGGGC CTCCAGCATC 
TACAGCTCCT GGGCAGAGGA GCAGCGTCGC CTGGAGCCTG CAGGGAAGAA GATTGAGGCC 
GACTCCCAGA CACTGTGGAC CAGCTGCTGG TGCCCACTGC TCCAAGGGAT AGCCTGGCTG 
TGCTGCGATG CCAGGAGGCT GGTGCGAATG CAGGCCCTGA CCTACCTCCA GAGGGCACTG 
TTGGTCCATG ACCTGCAGAC ACTAGATGCT GTGGAGTGGG AGTCCTGCTT CAACAAGGTG 
CTGTTCCCCC TGCTGACCAA GCTTCTGGAC AATATTAGCC CTGCAGACGT GTGTGGCATG 
GAGGAGACCA GGATGAGGGC TTGCACCCTG CTGTCAAAGG TGTTCCTGCA GCACCTCTCT 
CCCCTGTTGT CTCTGTCCAC CTTCGCTGCC CTGTGGCTCA CCATCCTGGA CTTCATGGAC 
AAGTACATGC ACGCTGGCTC CAGTGACCTG CTGCTGGAGG CCATCCCAGA GTCTCTGAAG 
AACATGCTGC TGGTGATGGA CACAGCAGGA ATCTTCCACA GCGCTGACTC TCGTACGGGA 
TACTCTGACC TGTGGGAGAT CACCTGGGAA CGCATCGACT GCTTCCTGCC TAACCTCCGG 
GAGGAGCTCT TCAAACAGAC AGTCATACCA CAACCAGATC CTGTTCCTAG TCCCCCAGTG 
GAGCTTGTCC AGCCGGCTGC CCCCTGCCCC CCAGCCTCGC CACCCCCAGC TCAGGCCCCA 
TCTCTTGTGC CAGTGCCAGC TGCCCCCACA GAGCCAAAGA CCTCCAGCAG ACCAGCGTCG 
CCCCAAGATC CCCCACCACC TAGTACCAAC GGAACAGACA GATCATCTCC AGTACCCCCG 
TCGACTCCCC CCATGCCCAC CCCAGTGAAG ATGTCCCCCC AGATCTCTAG CCCACTGATC 
CAGTCCCCCA CGAGTCAGTC CCCTCTCATC CTGCAGCCCC TGGCCTCCCA TCTGCAGGTG 
GGGGGCGTGC CCCCTATGTC CCTCCCCATT ATTCTCAACC CCGCCCTCAT TGAGGCCAGT 
TGCCCTGCTC CCCGCCCGCC ACCCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014058162.1
CDS	285..5834
Translation	MVDKNIYIVQGEICTVVGAIKRNSRWNTHTLLDEEQDPLLNSFSHLKEILNNMKELSDVEPNVFLRPFLEVVRSEDTTGPITGLALTSVNKFLSYGLIDANHEAAAEAIENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFSRLPQFKEEAKSFVGANMKKLKMRAGGMSESSKWKKQKRSPHPPRHMVRSASGGLEQAPTTTLSNNLLSGGVPFIEQDSLSVSDSVASSMSIPTDSGLDTCSKATSREDLTDLDQSSSAATTPSTEPGRLYTQSEGRQVEHAQSASVESIPEVLEDGESVTEHSDSASLHDMDYVNPRGVRFTPQQTQRDGAALIPYSLPCLRELFRFLISLTNPHDRHNTDAMMHMGLQLLTVALEAAHIAPYQSLLGLVKDELCRHLFQLLSVDRMNLYAASVRACFLLFESMRGHLKFQLEMYLKKLMDIITSENMKMLYEMKEMALEALVQLSRIPSFVTELYINYDCDFYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLEALLTVIDSTEAHCQAKVLNSSAQQDQSAEMVLAEGEGSTNDRTDTTTEASRQVNSANGQNVPEAHSWPLPPTSGHLMAEKMRLGRQDQEETEPAEKKEPKKPQRFSSCLPDSQELLDIRNKKKLLNSGTEQFNQKPKKGIQFLQEKGLLSSPMDNNEVAQWLRENPRLDKKQIGEFISDRKYMELLDSFVNTFTFRGLRIDEALRLYLEAFRLPGEAPVIHRLLETFTDTWHKVNGSPFMTNDAGFALAYAVIMLNTDQHNNNVRKQNIPMTIEQFKKNLKGVNGNTDFDQDMLEDIYNAIKNEEIVMPDEQSGLVKENYVWSVLLHRGATSEGVFLHVPAGSYDHDLFTMTWGPTIAALSYVFDKSQDDTIIQKAIAGFRKCAMISAHYGFSDVFDNLIISLCKFTILSSESVENLPTVFGSNGKAQIAAKTMFHLAHRHGDILREGWKNIMDSMLQLFRAELLPKAMLEVEDFVEPNGTISLQREETPSNRGESAVLSFVNWLTLSGAEQSGQRGPSTENQEAKQAAVLCIKQCDPEKLITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVMENRDRVSCVWQTVRDHLCHLCVQATESCFLVERAVVGLIRLAIRLLRREDISSQVLLSLRLLLMMKPHVLARVSREVAYGLHELLKTNAANIHCTDDWFTLFSLIECIGAGIKPPASFKVTSTTPDIDTGAQSDSELSFHHPSEVSLDRGYTSDSEVYTEHGKSRMPRSVTDVDVTSSGWLVVGKDDLDISKVAASGSKTLLNPLVNQYSLTLGQDMGFHDTKSLIKCVESLSFIVRDAAHVTPENFELCVKAIRVFVEASLNGGYRTYEKKKNHKYDSQKSRVRRKPRDKEGASRRAKASSQRPSRSHSDDEEDEGVPASYHMVSLQLLDLMHTLHTRASSIYSSWAEEQRRLEPAGKKIEADSQTLWTSCWCPLLQGIAWLCCDARRLVRMQALTYLQRALLVHDLQTLDAVEWESCFNKVLFPLLTKLLDNISPADVCGMEETRMRACTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLLEAIPESLKNMLLVMDTAGIFHSADSRTGYSDLWEITWERIDCFLPNLREELFKQTVIPQPDPVPSPPVELVQPAAPCPPASPPPAQAPSLVPVPAAPTEPKTSSRPASPQDPPPPSTNGTDRSSPVPPSTPPMPTPVKMSPQISSPLIQSPTSQSPLILQPLASHLQVGGVPPMSLPIILNPALIEASCPAPRPPPH

Target ORF information:

RefSeq Version	XM_014202687.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC106606442), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014202687.1

ORF Insert Sequence:

ATGGTGGACA AGAATATATA CATTGTCCAA GGGGAGATCT GTACTGTTGT AGGTGCCATC 
AAGAGGAATT CAAGGTGGAA TACCCATACT CTTTTGGATG AGGAACAAGA TCCATTGCTT 
AACAGTTTCA GCCACCTGAA AGAGATTCTC AACAATATGA AAGAGCTGTC GGATGTGGAG 
CCCAACGTAT TCCTGCGGCC CTTCCTGGAG GTAGTACGAT CTGAAGACAC CACCGGACCA 
ATCACAGGCC TGGCCCTCAC CTCTGTCAAC AAGTTCCTGT CCTACGGCCT CATAGATGCT 
AACCACGAGG CGGCGGCGGA GGCCATCGAG AACATGGCGG ATGCCGTCAC ACACGCCAGA 
TTCGTAGGGA CGGACCCCGC CAGCGATGAA GTAGTGCTCA TGAAAATCCT ACAGGTCCTG 
AGGACTTTGC TGCTCACGCC CGTAGGAGCC CACCTGACCA ACGAGTCTGT GTGTGAGATC 
ATGCAGTCGT GTTTCCGCAT CTGCTTCGAG ATGCGCCTCA GCGAGCTCCT GAGGAAATCA 
GCCGAACACA CACTGGTGGA CATGGTGCAG CTGCTGTTTT CCAGACTACC CCAGTTTAAA 
GAGGAGGCCA AAAGTTTTGT GGGCGCCAAC ATGAAGAAGC TGAAGATGCG TGCTGGTGGG 
ATGAGTGAGT CGTCAAAGTG GAAGAAGCAG AAGCGCTCGC CCCACCCCCC CCGTCACATG 
GTGCGAAGTG CCTCTGGCGG GTTGGAGCAG GCCCCTACCA CCACCCTCAG CAACAACCTC 
CTCTCTGGTG GTGTCCCATT CATCGAGCAG GATAGTTTGT CTGTCTCAGA CAGCGTGGCC 
TCCTCCATGT CCATCCCCAC AGACAGCGGC CTGGACACCT GCTCCAAGGC CACCTCCAGA 
GAGGACCTGA CAGACCTGGA CCAGAGCAGC TCTGCTGCCA CCACCCCTTC CACAGAGCCT 
GGACGCCTGT ACACTCAGTC TGAGGGTAGG CAGGTGGAGC ATGCCCAGTC AGCCTCGGTG 
GAGTCCATCC CTGAGGTCCT GGAGGACGGA GAATCTGTGA CGGAGCATTC GGACTCAGCC 
TCATTACATG ACATGGACTA TGTCAACCCC CGAGGAGTCC GCTTCACACC ACAGCAAACA 
CAGAGAGACG GTGCGGCTCT CATCCCCTAC TCCCTGCCCT GTCTGCGGGA GCTCTTCCGC 
TTCCTCATCT CACTGACCAA CCCCCATGAC CGCCACAACA CGGACGCCAT GATGCACATG 
GGCCTGCAGT TGCTGACCGT GGCTCTGGAG GCGGCTCACA TCGCCCCCTA CCAGTCACTG 
CTGGGCCTGG TCAAAGACGA GCTCTGCAGA CACCTCTTCC AGTTGCTAAG TGTGGACCGT 
ATGAACCTGT ATGCCGCCTC CGTCCGAGCG TGCTTCTTGC TCTTTGAGAG CATGCGAGGA 
CATTTGAAGT TTCAGCTCGA GATGTATCTG AAGAAGCTGA TGGACATCAT CACATCAGAG 
AACATGAAGA TGCTCTATGA GATGAAGGAG ATGGCCCTGG AGGCCCTGGT GCAGCTCTCG 
AGGATCCCCA GCTTCGTCAC CGAGCTCTAC ATCAATTACG ACTGTGACTT CTACTGCTCA 
AACCTGTTTG AAGACCTCAC CAAGCTGCTC TCTAAGAATG CCTTCCCTGT GTCCGGACAG 
CTCTACACAA CCCACCTCCT CTCACTAGAG GCCCTACTGA CTGTTATTGA CAGCACTGAG 
GCCCACTGCC AGGCCAAGGT GCTCAACAGC AGCGCTCAGC AGGACCAATC AGCAGAAATG 
GTGTTGGCAG AAGGGGAGGG CTCAACCAAC GACCGTACAG ACACAACCAC GGAAGCGAGC 
AGGCAGGTGA ACAGTGCCAA CGGTCAGAAT GTTCCAGAGG CTCACAGTTG GCCCCTACCA 
CCTACCAGCG GGCACCTGAT GGCAGAGAAG ATGAGACTGG GCAGACAAGA CCAGGAGGAG 
ACAGAACCAG CTGAGAAGAA AGAACCAAAA AAGCCACAAC GCTTCTCGTC TTGTTTACCT 
GATTCTCAAG AGCTGTTGGA CATCAGAAAT AAAAAGAAGC TGCTGAACAG CGGTACAGAG 
CAGTTCAACC AGAAGCCTAA GAAGGGCATC CAGTTCCTGC AGGAGAAGGG TCTCCTCAGC 
AGCCCCATGG ACAACAACGA GGTGGCCCAG TGGCTCCGGG AGAACCCCCG CCTCGACAAG 
AAGCAGATCG GAGAGTTCAT CAGTGACCGC AAATACATGG AGTTACTGGA CAGCTTCGTC 
AACACGTTCA CCTTCCGAGG GCTGCGTATT GACGAGGCGC TGCGTCTGTA TCTGGAGGCC 
TTCAGACTGC CAGGAGAGGC TCCGGTCATC CATAGACTTT TGGAAACCTT CACAGACACC 
TGGCATAAAG TGAACGGCTC TCCCTTCATG ACCAATGACG CAGGCTTCGC CCTGGCCTAT 
GCTGTCATCA TGCTAAACAC GGACCAGCAC AACAACAACG TGCGCAAACA GAACATCCCC 
ATGACCATTG AGCAATTCAA GAAGAATCTG AAGGGTGTGA ATGGAAATAC AGACTTTGAT 
CAGGATATGC TGGAAGACAT CTACAATGCA ATCAAGAATG AAGAGATAGT GATGCCAGAC 
GAGCAGTCTG GCCTGGTTAA GGAGAACTAT GTATGGAGCG TGCTGCTCCA CCGGGGGGCC 
ACCTCCGAGG GGGTCTTCCT TCATGTGCCC GCCGGCAGCT ACGACCACGA CCTGTTCACC 
ATGACCTGGG GGCCCACCAT CGCAGCCCTC TCCTACGTCT TCGACAAGAG CCAGGACGAC 
ACAATCATCC AGAAGGCCAT TGCTGGCTTT AGGAAGTGTG CCATGATATC AGCTCATTAT 
GGATTCAGTG ACGTTTTTGA TAATTTGATC ATCTCCCTCT GCAAGTTTAC AATACTCAGC 
AGTGAGTCGG TGGAGAACCT GCCCACAGTA TTTGGGAGTA ACGGGAAGGC ACAGATAGCG 
GCGAAAACGA TGTTCCACCT GGCCCACCGG CACGGTGACA TCCTGAGGGA AGGCTGGAAG 
AACATCATGG ACTCCATGCT GCAGCTGTTC AGAGCCGAGC TGCTGCCCAA GGCCATGCTG 
GAGGTGGAGG ATTTTGTGGA GCCAAATGGA ACGATTTCCC TTCAAAGAGA AGAGACACCG 
TCAAATCGGG GTGAGTCTGC GGTGCTGAGC TTTGTGAACT GGCTGACACT GAGTGGAGCG 
GAGCAGTCTG GTCAACGTGG GCCCTCTACT GAGAACCAGG AGGCCAAGCA GGCAGCCGTG 
CTCTGTATCA AGCAATGTGA CCCAGAGAAG CTGATCACAG AGAGTAAATT CCTGCAGCTG 
GAGTCTCTAC AGGAGCTGAT GAAGGCCCTG ATCTCAGTCA CACCTGATGA GGAGACGTAT 
GACGAGGAGG ATGCTGCGTT CTGCCTGGAG ATGCTGTTAC GCATAGTCAT GGAGAACAGG 
GACCGTGTGT CGTGTGTATG GCAGACGGTG CGGGATCACC TGTGCCACCT GTGTGTCCAG 
GCTACAGAGA GCTGCTTCCT GGTGGAGAGG GCCGTGGTGG GCCTCATAAG ACTGGCCATC 
CGCCTGCTGC GCAGAGAGGA CATCAGCTCC CAGGTGCTGC TGTCCCTGCG TCTCCTGCTG 
ATGATGAAAC CACACGTCCT GGCCCGGGTT AGCCGGGAGG TGGCCTACGG CCTCCATGAG 
CTGCTTAAGA CCAACGCTGC CAACATCCAC TGCACAGACG ACTGGTTCAC CCTTTTCTCC 
CTAATCGAGT GCATCGGGGC CGGGATCAAA CCCCCCGCCT CCTTCAAGGT CACCTCCACT 
ACCCCAGACA TCGACACAGG TGCCCAGTCG GACAGTGAGC TGTCATTCCA CCACCCCAGT 
GAGGTGAGTC TGGACCGGGG GTACACCTCA GACTCTGAGG TCTACACGGA GCACGGAAAG 
TCCAGGATGC CCCGCTCTGT CACTGACGTG GACGTGACCA GCAGCGGATG GCTGGTGGTT 
GGGAAAGACG ACCTGGACAT CAGCAAGGTA GCGGCTTCTG GCTCCAAGAC CCTGCTAAAC 
CCTCTAGTGA ACCAGTACAG CCTGACCCTG GGTCAAGACA TGGGTTTCCA CGACACCAAG 
TCTCTCATCA AGTGTGTGGA GTCCCTCTCC TTCATCGTAC GCGACGCTGC ACACGTCACC 
CCCGAGAACT TTGAGCTGTG TGTCAAGGCC ATCCGGGTGT TTGTGGAGGC CAGCCTAAAC 
GGAGGCTACA GGACCTATGA GAAGAAGAAG AACCACAAGT ACGACTCCCA GAAGTCCCGT 
GTGAGGAGGA AGCCCCGGGA CAAGGAGGGG GCATCCCGCC GCGCCAAGGC CTCCAGCCAG 
AGGCCTTCCC GTTCCCATAG CGATGACGAG GAGGACGAGG GGGTCCCAGC CAGCTACCAC 
ATGGTGTCAT TACAGCTGTT AGATCTGATG CACACGCTGC ATACGAGGGC CTCCAGCATC 
TACAGCTCCT GGGCAGAGGA GCAGCGTCGC CTGGAGCCTG CAGGGAAGAA GATTGAGGCC 
GACTCCCAGA CACTGTGGAC CAGCTGCTGG TGCCCACTGC TCCAAGGGAT AGCCTGGCTG 
TGCTGCGATG CCAGGAGGCT GGTGCGAATG CAGGCCCTGA CCTACCTCCA GAGGGCACTG 
TTGGTCCATG ACCTGCAGAC ACTAGATGCT GTGGAGTGGG AGTCCTGCTT CAACAAGGTG 
CTGTTCCCCC TGCTGACCAA GCTTCTGGAC AATATTAGCC CTGCAGACGT GTGTGGCATG 
GAGGAGACCA GGATGAGGGC TTGCACCCTG CTGTCAAAGG TGTTCCTGCA GCACCTCTCT 
CCCCTGTTGT CTCTGTCCAC CTTCGCTGCC CTGTGGCTCA CCATCCTGGA CTTCATGGAC 
AAGTACATGC ACGCTGGCTC CAGTGACCTG CTGCTGGAGG CCATCCCAGA GTCTCTGAAG 
AACATGCTGC TGGTGATGGA CACAGCAGGA ATCTTCCACA GCGCTGACTC TCGTACGGGA 
TACTCTGACC TGTGGGAGAT CACCTGGGAA CGCATCGACT GCTTCCTGCC TAACCTCCGG 
GAGGAGCTCT TCAAACAGAC AGTCATACCA CAACCAGATC CTGTTCCTAG TCCCCCAGTG 
GAGCTTGTCC AGCCGGCTGC CCCCTGCCCC CCAGCCTCGC CACCCCCAGC TCAGGCCCCA 
TCTCTTGTGC CAGTGCCAGC TGCCCCCACA GAGCCAAAGA CCTCCAGCAG ACCAGCGTCG 
CCCCAAGATC CCCCACCACC TAGTACCAAC GGAACAGACA GATCATCTCC AGTACCCCCG 
TCGACTCCCC CCATGCCCAC CCCAGTGAAG ATGTCCCCCC AGATCTCTAG CCCACTGATC 
CAGTCCCCCA CGAGTCAGTC CCCTCTCATC CTGCAGCCCC TGGCCTCCCA TCTGCAGGTG 
GGGGGCGTGC CCCCTATGTC CCTCCCCATT ATTCTCAACC CCGCCCTCAT TGAGGCCAGT 
TGCCCTGCTC CCCGCCCGCC ACCCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106606442 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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