Nek8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nek8
Entrez Gene ID	287473
Full Name	NIMA-related kinase 8
General protein information	Preferred Names NIMA-related kinase 8 Names serine/threonine-protein kinase Nek8 NIMA (never in mitosis gene a)- related kinase 8 NIMA (never in mitosis gene a)-related expressed kinase 8 never in mitosis A-related kinase 8 nimA-related protein kinase 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q25

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246933.2	XP_006246995.1	serine/threonine-protein kinase Nek8 isoform X1
NM_001105804.1	NP_001099274.1	serine/threonine-protein kinase Nek8

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006872	XP_308885.4
Macaca mulatta (Rhesus monkey)	NEK8	XP_001106986.2
Drosophila melanogaster (fruit fly)	niki	NP_651293.1
Canis lupus familiaris (dog)	NEK8	XP_548291.3
Pan troglodytes (chimpanzee)	NEK8	XP_511801.3
Mus musculus (house mouse)	Nek8	NP_543125.1
Homo sapiens (human)	NEK8	NP_835464.1
Rattus norvegicus (Norway rat)	Nek8	NP_001099274.1
Danio rerio (zebrafish)	nek8	NP_620776.1
Bos taurus (cattle)	NEK8	NP_001179652.1
Gallus gallus (chicken)	NEK8	XP_415822.3
Caenorhabditis elegans (roundworm)	nekl-1	NP_490968.2
Xenopus tropicalis (tropical clawed frog)	nek8	NP_001006906.1

Gene Ontology
Bibliography

Related articles in PubMed

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

Mutations in NEK8 link multiple organ dysplasia with altered Hippo signalling and increased c-MYC expression.
Frank V, Habbig S, Bartram MP, Eisenberger T, Veenstra-Knol HE, Decker C, Boorsma RA, Göbel H, Nürnberg G, Griessmann A, Franke M, Borgal L, Kohli P, Völker LA, Dötsch J, Nürnberg P, Benzing T, Bolz HJ, Johnson C, Gerkes EH, Schermer B, Bergmann C
Human molecular genetics22(11)2177-85(2013 Jun)

A novel mutation causing nephronophthisis in the Lewis polycystic kidney rat localises to a conserved RCC1 domain in Nek8.
McCooke JK, Appels R, Barrero RA, Ding A, Ozimek-Kulik JE, Bellgard MI, Morahan G, Phillips JK
BMC genomics13393(2012 Aug)

Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of primary cilia.
Shiba D, Manning DK, Koga H, Beier DR, Yokoyama T
Cytoskeleton (Hoboken, N.J.)67(2)112-9(2010 Feb)

NEK8 mutations affect ciliary and centrosomal localization and may cause nephronophthisis.
Otto EA, Trapp ML, Schultheiss UT, Helou J, Quarmby LM, Hildebrandt F
Journal of the American Society of Nephrology : JASN19(3)587-92(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nek8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nek8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11447	NM_001105804.1 Latest version!	Rattus norvegicus NIMA-related kinase 8 (Nek8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa11447	XM_006246933.2 Latest version!	Rattus norvegicus NIMA-related kinase 8 (Nek8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nek8 ( NM_001105804.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099274.1 Rattus norvegicus NIMA-related kinase 8 (Nek8), mRNA.

Price & Availability↑

CloneID	ORa11447
Clone ID Related Accession (Same CDS sequence)	XM_006246933.2 , NM_001105804.1
Accession Version	NM_001105804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550332800000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase Nek8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 3, 2007 this sequence version replaced XM_220639.4, XM_001080757.1.

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTTCGAA AGGCTGACCA GAAGCTGGTG ATCATCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAGG AGAGGCAGGC GGCACAGAAT GAGTGCCAGG TGCTCAAACT GCTCAACCAC 
CCCAACGTCA TTGAGTACTA CGAGAACTTC CTGGAGGACA AGGCCCTCAT GATCGCCATG 
GAATATGCAC CAGGTGGCAC CCTGGCTGAG TTCATCCAGA AGCGCTGCAA CTCCCTGCTG 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TTGCGCTGCA TCATGTGCAC 
ACACACCTCA TCCTGCATCG GGACCTCAAG ACCCAGAACA TCCTTCTTGA CAAGCACCGC 
ATGGTCGTCA AGATCGGGGA CTTTGGCATC TCTAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACTCC ATGCTACATC TCCCCTGAGC TATGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTTGC CAGTCTCAAG 
AGGGCCTTTG AGGCAGCGAA CTTGCCCGCC CTGGTGCTGA AGATCATGAG CGGCACCTTT 
GCACCCATCT CTGACCGTTA TAGTCCTGAG CTACGCCAGC TTGTCCTGAG TCTGCTCAGC 
CTGGAACCTG CACAGCGGCC ACCCCTCAGC CACATCATGG CTCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTCCGCA TGCGGAGGGC AGAGAAGTGC 
CTGACCCCAG GAACACCTAT GGCCCCGGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGCAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCCATCC CGCCGCCTCT GTCTTCCGTC 
TACGCTTGGG GTGGTGGCCT TAGCATTCCC CTGAGGTTGC CGATGCCCAA CACGGAGGTG 
GTCCAGGTGG CCGCAGGGCG CACACAGAAG GCTGGCGTTA CACGCTCAGG ACGCCTCATT 
CTGTGGGAGG CCCCACCCCT GGGAACTGGA GGCGGCACAC TCCTTCCGGG GGCAGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAGGGCC AGTCAGGCGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACTGCT TGCTTGACGG ACAGAGGCAT CATCATGACT 
TTTGGCAGCG GCAGCAACGG GTGTTTAGGT CACGGCAGCC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AGGCCCTGCT GGGGTATGAG ATGGTGCAGG TGGCCTGTGG AGCCTCTCAT 
GTGCTGGCCC TGTCCGCAGA TGGAGAGTTA TTTGCCTGGG GCCGAGGAGA TGGCGGTAGG 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTAT GGTCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTGTTCCA TGATCCTCAC CTCACCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACTG CCTGTCCCTG 
GAGGAGGAGC CTGTTCCCCA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC GCCGCTGGGT 
TCTGCACCTC TGGACCGGGA GACCCTGCTG TGCGTGGACC TTGGCACTGC TCATTCAGCT 
GCTGTGACTG CTTCCGGGGC CTGCTATACT TTTGGCAGCA ATCAGCATGG GCAATTGGGC 
ACCAGTTCCC GCCGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTAGA GGGCATCAAG 
ATGGTGATGG TGGCCTGTGG GGATGCCTTC ACTGTGGCCA TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAGC CCGGGGTCGC CTGGGAAGGA GGGATGAGGA TGCCGGACTC 
CCTAGACCAG TGCAGCTGGA TGAGACTCAT CCTTACACGG TGACATCAGT GTCCTGCTGC 
CATGGGAACA CTCTTTTGGC TGTTCGATCA GTCACAGATG AACCAGTTCC CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099274.1
CDS	61..2157
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKCLTPGTPMAPGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSIPLRLPMPNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGTGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSADGELFAWGRGDGGRLGLGTRESHNCPQQVPMVPGQEAQRVVCGIDCSMILTSPGRVLACGSNRFNKLGLDCLSLEEEPVPHQQVEEALSFTPLGSAPLDRETLLCVDLGTAHSAAVTASGACYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	NM_001105804.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NIMA-related kinase 8 (Nek8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105804.1

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTTCGAA AGGCTGACCA GAAGCTGGTG ATCATCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAGG AGAGGCAGGC GGCACAGAAT GAGTGCCAGG TGCTCAAACT GCTCAACCAC 
CCCAACGTCA TTGAGTACTA CGAGAACTTC CTGGAGGACA AGGCCCTCAT GATCGCCATG 
GAATATGCAC CAGGTGGCAC CCTGGCTGAG TTCATCCAGA AGCGCTGCAA CTCCCTGCTG 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TTGCGCTGCA TCATGTGCAC 
ACACACCTCA TCCTGCATCG GGACCTCAAG ACCCAGAACA TCCTTCTTGA CAAGCACCGC 
ATGGTCGTCA AGATCGGGGA CTTTGGCATC TCTAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACTCC ATGCTACATC TCCCCTGAGC TATGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTTGC CAGTCTCAAG 
AGGGCCTTTG AGGCAGCGAA CTTGCCCGCC CTGGTGCTGA AGATCATGAG CGGCACCTTT 
GCACCCATCT CTGACCGTTA TAGTCCTGAG CTACGCCAGC TTGTCCTGAG TCTGCTCAGC 
CTGGAACCTG CACAGCGGCC ACCCCTCAGC CACATCATGG CTCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTCCGCA TGCGGAGGGC AGAGAAGTGC 
CTGACCCCAG GAACACCTAT GGCCCCGGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGCAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCCATCC CGCCGCCTCT GTCTTCCGTC 
TACGCTTGGG GTGGTGGCCT TAGCATTCCC CTGAGGTTGC CGATGCCCAA CACGGAGGTG 
GTCCAGGTGG CCGCAGGGCG CACACAGAAG GCTGGCGTTA CACGCTCAGG ACGCCTCATT 
CTGTGGGAGG CCCCACCCCT GGGAACTGGA GGCGGCACAC TCCTTCCGGG GGCAGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAGGGCC AGTCAGGCGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACTGCT TGCTTGACGG ACAGAGGCAT CATCATGACT 
TTTGGCAGCG GCAGCAACGG GTGTTTAGGT CACGGCAGCC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AGGCCCTGCT GGGGTATGAG ATGGTGCAGG TGGCCTGTGG AGCCTCTCAT 
GTGCTGGCCC TGTCCGCAGA TGGAGAGTTA TTTGCCTGGG GCCGAGGAGA TGGCGGTAGG 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTAT GGTCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTGTTCCA TGATCCTCAC CTCACCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACTG CCTGTCCCTG 
GAGGAGGAGC CTGTTCCCCA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC GCCGCTGGGT 
TCTGCACCTC TGGACCGGGA GACCCTGCTG TGCGTGGACC TTGGCACTGC TCATTCAGCT 
GCTGTGACTG CTTCCGGGGC CTGCTATACT TTTGGCAGCA ATCAGCATGG GCAATTGGGC 
ACCAGTTCCC GCCGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTAGA GGGCATCAAG 
ATGGTGATGG TGGCCTGTGG GGATGCCTTC ACTGTGGCCA TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAGC CCGGGGTCGC CTGGGAAGGA GGGATGAGGA TGCCGGACTC 
CCTAGACCAG TGCAGCTGGA TGAGACTCAT CCTTACACGG TGACATCAGT GTCCTGCTGC 
CATGGGAACA CTCTTTTGGC TGTTCGATCA GTCACAGATG AACCAGTTCC CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek8 ( XM_006246933.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246995.1 Rattus norvegicus NIMA-related kinase 8 (Nek8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11447
Clone ID Related Accession (Same CDS sequence)	XM_006246933.2 , NM_001105804.1
Accession Version	XM_006246933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase Nek8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTTCGAA AGGCTGACCA GAAGCTGGTG ATCATCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAGG AGAGGCAGGC GGCACAGAAT GAGTGCCAGG TGCTCAAACT GCTCAACCAC 
CCCAACGTCA TTGAGTACTA CGAGAACTTC CTGGAGGACA AGGCCCTCAT GATCGCCATG 
GAATATGCAC CAGGTGGCAC CCTGGCTGAG TTCATCCAGA AGCGCTGCAA CTCCCTGCTG 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TTGCGCTGCA TCATGTGCAC 
ACACACCTCA TCCTGCATCG GGACCTCAAG ACCCAGAACA TCCTTCTTGA CAAGCACCGC 
ATGGTCGTCA AGATCGGGGA CTTTGGCATC TCTAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACTCC ATGCTACATC TCCCCTGAGC TATGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTTGC CAGTCTCAAG 
AGGGCCTTTG AGGCAGCGAA CTTGCCCGCC CTGGTGCTGA AGATCATGAG CGGCACCTTT 
GCACCCATCT CTGACCGTTA TAGTCCTGAG CTACGCCAGC TTGTCCTGAG TCTGCTCAGC 
CTGGAACCTG CACAGCGGCC ACCCCTCAGC CACATCATGG CTCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTCCGCA TGCGGAGGGC AGAGAAGTGC 
CTGACCCCAG GAACACCTAT GGCCCCGGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGCAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCCATCC CGCCGCCTCT GTCTTCCGTC 
TACGCTTGGG GTGGTGGCCT TAGCATTCCC CTGAGGTTGC CGATGCCCAA CACGGAGGTG 
GTCCAGGTGG CCGCAGGGCG CACACAGAAG GCTGGCGTTA CACGCTCAGG ACGCCTCATT 
CTGTGGGAGG CCCCACCCCT GGGAACTGGA GGCGGCACAC TCCTTCCGGG GGCAGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAGGGCC AGTCAGGCGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACTGCT TGCTTGACGG ACAGAGGCAT CATCATGACT 
TTTGGCAGCG GCAGCAACGG GTGTTTAGGT CACGGCAGCC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AGGCCCTGCT GGGGTATGAG ATGGTGCAGG TGGCCTGTGG AGCCTCTCAT 
GTGCTGGCCC TGTCCGCAGA TGGAGAGTTA TTTGCCTGGG GCCGAGGAGA TGGCGGTAGG 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTAT GGTCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTGTTCCA TGATCCTCAC CTCACCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACTG CCTGTCCCTG 
GAGGAGGAGC CTGTTCCCCA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC GCCGCTGGGT 
TCTGCACCTC TGGACCGGGA GACCCTGCTG TGCGTGGACC TTGGCACTGC TCATTCAGCT 
GCTGTGACTG CTTCCGGGGC CTGCTATACT TTTGGCAGCA ATCAGCATGG GCAATTGGGC 
ACCAGTTCCC GCCGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTAGA GGGCATCAAG 
ATGGTGATGG TGGCCTGTGG GGATGCCTTC ACTGTGGCCA TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAGC CCGGGGTCGC CTGGGAAGGA GGGATGAGGA TGCCGGACTC 
CCTAGACCAG TGCAGCTGGA TGAGACTCAT CCTTACACGG TGACATCAGT GTCCTGCTGC 
CATGGGAACA CTCTTTTGGC TGTTCGATCA GTCACAGATG AACCAGTTCC CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246995.1
CDS	1977..4073
Translation	MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKCLTPGTPMAPGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSIPLRLPMPNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGTGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSADGELFAWGRGDGGRLGLGTRESHNCPQQVPMVPGQEAQRVVCGIDCSMILTSPGRVLACGSNRFNKLGLDCLSLEEEPVPHQQVEEALSFTPLGSAPLDRETLLCVDLGTAHSAAVTASGACYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

Target ORF information:

RefSeq Version	XM_006246933.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NIMA-related kinase 8 (Nek8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246933.2

ORF Insert Sequence:

ATGGAGAAGT ACGAGCGGAT CCGAGTGGTG GGAAGAGGTG CCTTCGGGAT TGTGCACCTG 
TGCCTTCGAA AGGCTGACCA GAAGCTGGTG ATCATCAAGC AGATCCCAGT GGAACAGATG 
ACCAAAGAGG AGAGGCAGGC GGCACAGAAT GAGTGCCAGG TGCTCAAACT GCTCAACCAC 
CCCAACGTCA TTGAGTACTA CGAGAACTTC CTGGAGGACA AGGCCCTCAT GATCGCCATG 
GAATATGCAC CAGGTGGCAC CCTGGCTGAG TTCATCCAGA AGCGCTGCAA CTCCCTGCTG 
GAGGAGGAGA CGATCCTTCA CTTCTTTGTG CAGATCCTGC TTGCGCTGCA TCATGTGCAC 
ACACACCTCA TCCTGCATCG GGACCTCAAG ACCCAGAACA TCCTTCTTGA CAAGCACCGC 
ATGGTCGTCA AGATCGGGGA CTTTGGCATC TCTAAGATCC TTAGCAGCAA GAGCAAGGCC 
TACACGGTGG TGGGTACTCC ATGCTACATC TCCCCTGAGC TATGTGAGGG CAAGCCCTAC 
AACCAGAAGA GTGACATCTG GGCTCTGGGC TGTGTCTTAT ATGAGCTTGC CAGTCTCAAG 
AGGGCCTTTG AGGCAGCGAA CTTGCCCGCC CTGGTGCTGA AGATCATGAG CGGCACCTTT 
GCACCCATCT CTGACCGTTA TAGTCCTGAG CTACGCCAGC TTGTCCTGAG TCTGCTCAGC 
CTGGAACCTG CACAGCGGCC ACCCCTCAGC CACATCATGG CTCAGCCCCT CTGCATCCGG 
GCCCTACTCA ACATCCACAC CGATGTGGGC AGCGTCCGCA TGCGGAGGGC AGAGAAGTGC 
CTGACCCCAG GAACACCTAT GGCCCCGGGC AGCACAGGGA GCAGGGCCAC CAGTGCCCGA 
TGCAGGGGTG TCCCCCGGGG ACCTGTGAGA CCGGCCATCC CGCCGCCTCT GTCTTCCGTC 
TACGCTTGGG GTGGTGGCCT TAGCATTCCC CTGAGGTTGC CGATGCCCAA CACGGAGGTG 
GTCCAGGTGG CCGCAGGGCG CACACAGAAG GCTGGCGTTA CACGCTCAGG ACGCCTCATT 
CTGTGGGAGG CCCCACCCCT GGGAACTGGA GGCGGCACAC TCCTTCCGGG GGCAGTGGAG 
CTGCCTCAGC CCCAGTTTGT CTCTCGTTTC CTGGAGGGCC AGTCAGGCGT GACCATCAAG 
CATGTGGCCT GCGGGGACCT GTTCACTGCT TGCTTGACGG ACAGAGGCAT CATCATGACT 
TTTGGCAGCG GCAGCAACGG GTGTTTAGGT CACGGCAGCC TGACTGACAT CAGCCAGCCC 
ACCATTGTAG AGGCCCTGCT GGGGTATGAG ATGGTGCAGG TGGCCTGTGG AGCCTCTCAT 
GTGCTGGCCC TGTCCGCAGA TGGAGAGTTA TTTGCCTGGG GCCGAGGAGA TGGCGGTAGG 
CTGGGACTAG GCACCAGGGA GTCCCATAAC TGCCCTCAGC AGGTGCCTAT GGTCCCAGGA 
CAGGAAGCTC AGAGAGTTGT CTGTGGTATT GACTGTTCCA TGATCCTCAC CTCACCAGGC 
AGGGTCCTGG CCTGTGGAAG TAACAGGTTC AACAAGCTAG GCTTGGACTG CCTGTCCCTG 
GAGGAGGAGC CTGTTCCCCA CCAGCAAGTG GAGGAGGCCC TGAGCTTCAC GCCGCTGGGT 
TCTGCACCTC TGGACCGGGA GACCCTGCTG TGCGTGGACC TTGGCACTGC TCATTCAGCT 
GCTGTGACTG CTTCCGGGGC CTGCTATACT TTTGGCAGCA ATCAGCATGG GCAATTGGGC 
ACCAGTTCCC GCCGGGTCAG CAGGGCACCC TGTCGGGTCC AAGGCCTAGA GGGCATCAAG 
ATGGTGATGG TGGCCTGTGG GGATGCCTTC ACTGTGGCCA TTGGGGCAGA GGGGGAAGTG 
TATTCTTGGG GCAAAGGAGC CCGGGGTCGC CTGGGAAGGA GGGATGAGGA TGCCGGACTC 
CCTAGACCAG TGCAGCTGGA TGAGACTCAT CCTTACACGG TGACATCAGT GTCCTGCTGC 
CATGGGAACA CTCTTTTGGC TGTTCGATCA GTCACAGATG AACCAGTTCC CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

Mutations in NEK8 link multiple organ dysplasia with altered Hippo signalling and increased c-MYC expression.
Human molecular genetics22(11)2177-85(2013 Jun)
Frank V,Habbig S,Bartram MP,Eisenberger T,Veenstra-Knol HE,Decker C,Boorsma RA,Göbel H,Nürnberg G,Griessmann A,Franke M,Borgal L,Kohli P,Völker LA,Dötsch J,Nürnberg P,Benzing T,Bolz HJ,Johnson C,Gerkes EH,Schermer B,Bergmann C

A novel mutation causing nephronophthisis in the Lewis polycystic kidney rat localises to a conserved RCC1 domain in Nek8.
BMC genomics13393(2012 Aug)
McCooke JK,Appels R,Barrero RA,Ding A,Ozimek-Kulik JE,Bellgard MI,Morahan G,Phillips JK

Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of primary cilia.
Cytoskeleton (Hoboken, N.J.)67(2)112-9(2010 Feb)
Shiba D,Manning DK,Koga H,Beier DR,Yokoyama T

NEK8 mutations affect ciliary and centrosomal localization and may cause nephronophthisis.
Journal of the American Society of Nephrology : JASN19(3)587-92(2008 Mar)
Otto EA,Trapp ML,Schultheiss UT,Helou J,Quarmby LM,Hildebrandt F

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