Hcrt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcrt
Entrez Gene ID	15171
Full Name	hypocretin
Synonyms	PPOX
General protein information	Preferred Names hypocretin Names orexin orexin A orexin B prepro-orexin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 D\|11 63.6 cM
Summary	This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors Hcrtr1 and Hcrtr2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010410.2	NP_034540.1	orexin precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HCRT	XP_001166578.1
Macaca mulatta (Rhesus monkey)	HCRT	XP_001108991.1
Homo sapiens (human)	HCRT	NP_001515.1
Bos taurus (cattle)	HCRT	NP_001159992.1
Rattus norvegicus (Norway rat)	Hcrt	NP_037311.1
Mus musculus (house mouse)	Hcrt	NP_034540.1
Canis lupus familiaris (dog)	HCRT	NP_001029166.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential Roles of Each Orexin Receptor Signaling in Obesity.
Kakizaki M, Tsuneoka Y, Takase K, Kim SJ, Choi J, Ikkyu A, Abe M, Sakimura K, Yanagisawa M, Funato H
iScience201-13(2019 Oct)

Molecular codes and in vitro generation of hypocretin and melanin concentrating hormone neurons.
Seifinejad A, Li S, Mikhail C, Vassalli A, Pradervand S, Arribat Y, Pezeshgi Modarres H, Allen B, John RM, Amati F, Tafti M
Proceedings of the National Academy of Sciences of the United States of America116(34)17061-17070(2019 Aug)

Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy.
Sun Y, Blanco-Centurion C, Bendell E, Vidal-Ortiz A, Luo S, Liu M
eLife8(2019 08)

Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice.
Chowdhury S, Hung CJ, Izawa S, Inutsuka A, Kawamura M, Kawashima T, Bito H, Imayoshi I, Abe M, Sakimura K, Yamanaka A
eLife8(2019 06)

GABA neurons in the ventral tegmental area regulate non-rapid eye movement sleep in mice.
Chowdhury S, Matsubara T, Miyazaki T, Ono D, Fukatsu N, Abe M, Sakimura K, Sudo Y, Yamanaka A
eLife8(2019 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hcrt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcrt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21796	NM_010410.2 Latest version!	Mus musculus hypocretin (Hcrt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcrt ( NM_010410.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034540.1 Mus musculus hypocretin (Hcrt), mRNA.

Price & Availability↑

CloneID	OMu21796
Clone ID Related Accession (Same CDS sequence)	NM_010410.2
Accession Version	NM_010410.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574697600000
Organism	Mus musculus(house mouse)
Product	orexin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF019566.1. On Apr 5, 2007 this sequence version replaced NM_010410.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF019566.1, CD769124.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTTTC CTTCTACAAA GGTTCCCTGG GCCGCCGTGA CGCTGCTGCT GCTGCTACTG 
CTGCCGCCGG CGCTGCTGTC GCTTGGGGTG GACGCACAGC CTCTGCCCGA CTGCTGTCGC 
CAGAAGACGT GTTCCTGCCG TCTCTACGAA CTGTTGCACG GAGCTGGCAA CCACGCTGCG 
GGTATCCTGA CTCTGGGAAA GCGGCGGCCT GGACCTCCAG GCCTCCAGGG ACGGCTGCAG 
CGCCTCCTTC AGGCCAACGG TAACCACGCA GCTGGCATCC TGACCATGGG CCGCCGCGCA 
GGCGCAGAGC TAGAGCCACA TCCCTGCTCT GGTCGCGGCT GTCCGACCGT AACTACCACC 
GCTTTAGCAC CCCGGGGAGG GTCCGGAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034540.1
CDS	91..483
Translation	MNFPSTKVPWAAVTLLLLLLLPPALLSLGVDAQPLPDCCRQKTCSCRLYELLHGAGNHAAGILTLGKRRPGPPGLQGRLQRLLQANGNHAAGILTMGRRAGAELEPHPCSGRGCPTVTTTALAPRGGSGV

Target ORF information:

RefSeq Version	NM_010410.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypocretin (Hcrt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010410.2

ORF Insert Sequence:

ATGAACTTTC CTTCTACAAA GGTTCCCTGG GCCGCCGTGA CGCTGCTGCT GCTGCTACTG 
CTGCCGCCGG CGCTGCTGTC GCTTGGGGTG GACGCACAGC CTCTGCCCGA CTGCTGTCGC 
CAGAAGACGT GTTCCTGCCG TCTCTACGAA CTGTTGCACG GAGCTGGCAA CCACGCTGCG 
GGTATCCTGA CTCTGGGAAA GCGGCGGCCT GGACCTCCAG GCCTCCAGGG ACGGCTGCAG 
CGCCTCCTTC AGGCCAACGG TAACCACGCA GCTGGCATCC TGACCATGGG CCGCCGCGCA 
GGCGCAGAGC TAGAGCCACA TCCCTGCTCT GGTCGCGGCT GTCCGACCGT AACTACCACC 
GCTTTAGCAC CCCGGGGAGG GTCCGGAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential Roles of Each Orexin Receptor Signaling in Obesity.
iScience201-13(2019 Oct)
Kakizaki M,Tsuneoka Y,Takase K,Kim SJ,Choi J,Ikkyu A,Abe M,Sakimura K,Yanagisawa M,Funato H

Molecular codes and in vitro generation of hypocretin and melanin concentrating hormone neurons.
Proceedings of the National Academy of Sciences of the United States of America116(34)17061-17070(2019 Aug)
Seifinejad A,Li S,Mikhail C,Vassalli A,Pradervand S,Arribat Y,Pezeshgi Modarres H,Allen B,John RM,Amati F,Tafti M

Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy.
eLife8(2019 08)
Sun Y,Blanco-Centurion C,Bendell E,Vidal-Ortiz A,Luo S,Liu M

Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice.
eLife8(2019 06)
Chowdhury S,Hung CJ,Izawa S,Inutsuka A,Kawamura M,Kawashima T,Bito H,Imayoshi I,Abe M,Sakimura K,Yamanaka A

GABA neurons in the ventral tegmental area regulate non-rapid eye movement sleep in mice.
eLife8(2019 06)
Chowdhury S,Matsubara T,Miyazaki T,Ono D,Fukatsu N,Abe M,Sakimura K,Sudo Y,Yamanaka A

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Hcrt cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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