ORF » Species Summary » Leishmania major strain Friedlin » LMJF_31_2250 cDNA ORF clone

LMJF_31_2250 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_31_2250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_31_2250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb04717 XM_001685160.1
Latest version!		 Leishmania major strain Friedlin putative 3,2-trans-enoyl-CoA isomerase,mitochondrial precursor partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_31_2250 ( XM_001685160.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001685212.1 Leishmania major strain Friedlin putative 3,2-trans-enoyl-CoA isomerase,mitochondrial precursor partial mRNA.
    Price & Availability↑
    CloneID OLb04717
    Clone ID Related Accession (Same CDS sequence) XM_001685160.1
    Accession Version XM_001685160.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative 3,2-trans-enoyl-CoA isomerase,mitochondrial precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007272). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAGTCGT CTCTTCGCCG CCGCGTCCGC CAGGCCTCCA ACGAAGACCC ACCATATCCC 
    ACATTCCTGT CGCACCGCCT TTTTGTTTGC TGTCGTCGCA GCACTTCCGC GATCATGCGC 
    CGTGTCATCT CGTCTTCTCT CACCCGTGGC GCCGGCACTC TTGGCGTCAT GTCGGGGCTG 
    TTTCAGCTCA CGCAGTACCG CTTCCAGTCG CAGCCACCGC CCGGTGTGCC GCAGGGCCGG 
    CATCAAGACC TGAATGCCGC TCATGCGGGA TCGAAGCACA GCGACGATGC GCAGCCAGAG 
    TTTGTGCCCC CAAAGAAGCC GACAGAGGTG CCCAAGCTTG TGAGAATCAA CACGAATGAG 
    AAGGGCATCA CAAACGTGCA GCTGGCCCGT GCCCCGGTGA ACTCGATGAG CCTGGAGCTC 
    TTCCAGGAGC TGAACCAGTG GATGCTGTGG CTTGGGAGCA ACGAGGAGAC GAAGGCTGTG 
    ATCATCTCCT CTGCGATTCC GACAGTCTTC TCAGCTGGAC TGGACCTCGT CGAGGTGCAT 
    AACCCGAAGC CGGATCGCGT CGCGGTCTTC TGGCAGAGCT TCCAGGAGAT GTGGCTCATC 
    CTCAATTCCT TCCCCAAGCC GATCATCGCG GCGATTACCG GCAACTCTCC GGCGGCGGGC 
    TGTATCATCG CGATGGGCTG CGACTACCGC GTGATGGCTC GCGGACCGAA GGGCAACACG 
    AACAACAACC GACTCTACCG CATCGGTCTT AACGAAACGA AACTAGGACT TGTTGCCCCA 
    CCGTGGGTGA TGCCAGCCTA CGCCTACCTG CTGGGCTCGC GCCAGGCGGA GCGGATGCTG 
    CAGCTGGGCG AGACACCCGT GGCCGATGAT GCACGAAATC TCGGGCTCAT TGATGAGGTG 
    GCCGCCGATG AGGAGCGGGC AATCGAAGTG GCCTACCAGC AGGCGGAACG CTTTCTCAGC 
    GTGCCGCAGC AGTCTCGGTG GATGGCCCGT GATATGATGC GACGCGAGTA CCTGCAGATG 
    CTTGCCTCGG ACGAAGAGCG CAACTACGAT ACAGAGTTTT TCACGCAATT TGTGCAGAGC 
    CCTGAGGTGC AGAAGAACCT TGAAAGCTAC CTGGAACGCC TCAAGAGCTG CTCTCGTAAG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001685212.1
    CDS1..1143
    Translation

    Target ORF information:

    RefSeq Version XM_001685160.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative 3,2-trans-enoyl-CoA isomerase,mitochondrial precursor partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001685160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAGTCGT CTCTTCGCCG CCGCGTCCGC CAGGCCTCCA ACGAAGACCC ACCATATCCC 
    ACATTCCTGT CGCACCGCCT TTTTGTTTGC TGTCGTCGCA GCACTTCCGC GATCATGCGC 
    CGTGTCATCT CGTCTTCTCT CACCCGTGGC GCCGGCACTC TTGGCGTCAT GTCGGGGCTG 
    TTTCAGCTCA CGCAGTACCG CTTCCAGTCG CAGCCACCGC CCGGTGTGCC GCAGGGCCGG 
    CATCAAGACC TGAATGCCGC TCATGCGGGA TCGAAGCACA GCGACGATGC GCAGCCAGAG 
    TTTGTGCCCC CAAAGAAGCC GACAGAGGTG CCCAAGCTTG TGAGAATCAA CACGAATGAG 
    AAGGGCATCA CAAACGTGCA GCTGGCCCGT GCCCCGGTGA ACTCGATGAG CCTGGAGCTC 
    TTCCAGGAGC TGAACCAGTG GATGCTGTGG CTTGGGAGCA ACGAGGAGAC GAAGGCTGTG 
    ATCATCTCCT CTGCGATTCC GACAGTCTTC TCAGCTGGAC TGGACCTCGT CGAGGTGCAT 
    AACCCGAAGC CGGATCGCGT CGCGGTCTTC TGGCAGAGCT TCCAGGAGAT GTGGCTCATC 
    CTCAATTCCT TCCCCAAGCC GATCATCGCG GCGATTACCG GCAACTCTCC GGCGGCGGGC 
    TGTATCATCG CGATGGGCTG CGACTACCGC GTGATGGCTC GCGGACCGAA GGGCAACACG 
    AACAACAACC GACTCTACCG CATCGGTCTT AACGAAACGA AACTAGGACT TGTTGCCCCA 
    CCGTGGGTGA TGCCAGCCTA CGCCTACCTG CTGGGCTCGC GCCAGGCGGA GCGGATGCTG 
    CAGCTGGGCG AGACACCCGT GGCCGATGAT GCACGAAATC TCGGGCTCAT TGATGAGGTG 
    GCCGCCGATG AGGAGCGGGC AATCGAAGTG GCCTACCAGC AGGCGGAACG CTTTCTCAGC 
    GTGCCGCAGC AGTCTCGGTG GATGGCCCGT GATATGATGC GACGCGAGTA CCTGCAGATG 
    CTTGCCTCGG ACGAAGAGCG CAACTACGAT ACAGAGTTTT TCACGCAATT TGTGCAGAGC 
    CCTGAGGTGC AGAAGAACCT TGAAAGCTAC CTGGAACGCC TCAAGAGCTG CTCTCGTAAG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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