MICU1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MICU1
Entrez Gene ID	10367
Full Name	mitochondrial calcium uptake 1
Synonyms	CALC,CBARA1,EFHA3,MPXPS
General protein information	Preferred Names mitochondrial calcium uptake 1 Names calcium uptake protein 1, mitochondrial ara CALC atopy-related autoantigen CALC calcium-binding atopy-related autoantigen 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.1
Summary	This gene encodes an essential regulator of mitochondrial Ca2+ uptake under basal conditions. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2013].
Disorder MIM:	605084
Disorder Html:	Myopathy with extrapyramidal signs, 615673 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005269386.2	XP_005269443.1	calcium uptake protein 1, mitochondrial isoform X3
NM_001195518.2	NP_001182447.1	calcium uptake protein 1, mitochondrial isoform 2
XM_011539119.1	XP_011537421.1	calcium uptake protein 1, mitochondrial isoform X1
NM_006077.3	NP_006068.2	calcium uptake protein 1, mitochondrial isoform 1
NM_001195519.1	NP_001182448.1	calcium uptake protein 1, mitochondrial isoform 3
XM_005269383.2	XP_005269440.1	calcium uptake protein 1, mitochondrial isoform X2
NM_001363513.1	NP_001350442.1	calcium uptake protein 1, mitochondrial isoform 4
NM_001195518.1	NP_001182447.1	calcium uptake protein 1, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MICU1	NP_001182447.1
Pan troglodytes (chimpanzee)	MICU1	XP_003339234.1
Canis lupus familiaris (dog)	MICU1	XP_005619058.1
Bos taurus (cattle)	MICU1	NP_001068806.1
Caenorhabditis elegans (roundworm)	micu-1	NP_001255723.1
Oryza sativa (rice)	Os02g0613000	NP_001047417.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009117	XP_319870.3
Xenopus tropicalis (tropical clawed frog)	micu1	NP_001106411.2
Oryza sativa (rice)	Os04g0502900	NP_001053237.1
Gallus gallus (chicken)	MICU1	XP_001231722.3
Drosophila melanogaster (fruit fly)	CG4495	NP_001097110.1
Mus musculus (house mouse)	Micu1	NP_659071.1
Rattus norvegicus (Norway rat)	Micu1	NP_955444.1
Danio rerio (zebrafish)	micu1	NP_001077302.1
Macaca mulatta (Rhesus monkey)	LOC708654	XP_001104987.2
Arabidopsis thaliana (thale cress)	AT4G32060	NP_194934.1

Gene Ontology
Bibliography

Related articles in PubMed

MICU1 controls cristae junction and spatially anchors mitochondrial Ca
Gottschalk B, Klec C, Leitinger G, Bernhart E, Rost R, Bischof H, Madreiter-Sokolowski CT, Radulović S, Eroglu E, Sattler W, Waldeck-Weiermair M, Malli R, Graier WF
Nature communications10(1)3732(2019 08)

Akt-mediated phosphorylation of MICU1 regulates mitochondrial Ca
Marchi S, Corricelli M, Branchini A, Vitto VAM, Missiroli S, Morciano G, Perrone M, Ferrarese M, Giorgi C, Pinotti M, Galluzzi L, Kroemer G, Pinton P
The EMBO journal38(2)(2019 01)

MICU1 Confers Protection from MCU-Dependent Manganese Toxicity.
Wettmarshausen J, Goh V, Huang KT, Arduino DM, Tripathi U, Leimpek A, Cheng Y, Pittis AA, Gabaldón T, Mokranjac D, Hajnóczky G, Perocchi F
Cell reports25(6)1425-1435.e7(2018 11)

MICU1 Interacts with the D-Ring of the MCU Pore to Control Its Ca
Paillard M, Csordás G, Huang KT, Várnai P, Joseph SK, Hajnóczky G
Molecular cell72(4)778-785.e3(2018 11)

Pathological consequences of MICU1 mutations on mitochondrial calcium signalling and bioenergetics.
Bhosale G, Sharpe JA, Koh A, Kouli A, Szabadkai G, Duchen MR
Biochimica et biophysica acta. Molecular cell research1864(6)1009-1017(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MICU1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MICU1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22305	NM_006077.3 Latest version!	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu22447	NM_001195519.1 Latest version!	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu43919	NM_001363513.1 Latest version!	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu43919	XM_005269383.2 Latest version!	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu43921	XM_005269386.2 Latest version!	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu63947	XM_011539119.1 Latest version!	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu22187	NM_001195518.2 Latest version!	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu22187	NM_001195518.1	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MICU1 ( NM_006077.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006068.2 Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22305
Clone ID Related Accession (Same CDS sequence)	NM_006077.3
Accession Version	NM_006077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	calcium uptake protein 1, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513185.12, AL117423.1, AC091769.6 and BU627477.1. This sequence is a reference standard in the RefSeqGene project. On Sep 14, 2010 this sequence version replaced NM_006077.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.115938.1, SRR1803614.90877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGACA 
GAGAAAATTT CCCAGGAACG AGAAAAATTT GCTGATGAAG GCAGTATATT TTACACCCTT 
GGAGAATGTG GGCTCATATC CTTTTCAGAC TACATTTTCC TCACAACTGT TCTTTCCACT 
CCTCAGAGAA ATTTTGAAAT TGCCTTCAAG ATGTTTGATT TGAATGGAGA TGGAGAAGTA 
GATATGGAAG AATTTGAACA GGTTCAGAGC ATCATTCGCT CCCAAACCAG TATGGGTATG 
CGCCACAGAG ATCGTCCAAC TACTGGCAAC ACCCTCAAGT CTGGCTTGTG TTCAGCCCTC 
ACAACCTACT TTTTTGGAGC TGATCTGAAG GGAAAGCTGA CAATCAAAAA CTTCCTCGAA 
TTTCAGCGTA AACTGCAGCA TGATGTTCTG AAGCTTGAGT TTGAACGCCA TGACCCTGTG 
GATGGGAGAA TTACTGAGAG GCAGTTTGGT GGCATGCTAC TTGCCTACAG TGGGGTGCAG 
TCCAAGAAGC TGACCGCCAT GCAGAGGCAG CTCAAGAAGC ACTTCAAAGA AGGAAAGGGT 
CTGACATTTC AGGAGGTGGA GAACTTCTTT ACTTTCCTAA AGAACATTAA TGATGTGGAC 
ACTGCATTGA GTTTTTACCA TATGGCTGGA GCATCTCTTG ATAAAGTGAC CATGCAGCAG 
GTGGCCAGGA CAGTGGCTAA AGTGGAGCTC TCAGACCACG TGTGTGATGT GGTGTTTGCA 
CTCTTTGACT GTGATGGCAA TGGCGAACTG AGCAATAAGG AATTTGTTTC CATCATGAAG 
CAACGGCTGA TGAGAGGCCT GGAAAAGCCC AAAGACATGG GTTTCACTCG CCTCATGCAG 
GCCATGTGGA AATGTGCACA GGAAACTGCC TGGGACTTCG CTTTACCCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006068.2
CDS	184..1620
Translation	MFRLNSLSALAELAVGSRWYHGGSQPIQIRRRLMMVAFLGASAVTASTGLLWKRAHAESPPCVDNLKSDIGDKGKNKDEGDVCNHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKTEKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

Target ORF information:

RefSeq Version	NM_006077.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006077.3

ORF Insert Sequence:

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGACA 
GAGAAAATTT CCCAGGAACG AGAAAAATTT GCTGATGAAG GCAGTATATT TTACACCCTT 
GGAGAATGTG GGCTCATATC CTTTTCAGAC TACATTTTCC TCACAACTGT TCTTTCCACT 
CCTCAGAGAA ATTTTGAAAT TGCCTTCAAG ATGTTTGATT TGAATGGAGA TGGAGAAGTA 
GATATGGAAG AATTTGAACA GGTTCAGAGC ATCATTCGCT CCCAAACCAG TATGGGTATG 
CGCCACAGAG ATCGTCCAAC TACTGGCAAC ACCCTCAAGT CTGGCTTGTG TTCAGCCCTC 
ACAACCTACT TTTTTGGAGC TGATCTGAAG GGAAAGCTGA CAATCAAAAA CTTCCTCGAA 
TTTCAGCGTA AACTGCAGCA TGATGTTCTG AAGCTTGAGT TTGAACGCCA TGACCCTGTG 
GATGGGAGAA TTACTGAGAG GCAGTTTGGT GGCATGCTAC TTGCCTACAG TGGGGTGCAG 
TCCAAGAAGC TGACCGCCAT GCAGAGGCAG CTCAAGAAGC ACTTCAAAGA AGGAAAGGGT 
CTGACATTTC AGGAGGTGGA GAACTTCTTT ACTTTCCTAA AGAACATTAA TGATGTGGAC 
ACTGCATTGA GTTTTTACCA TATGGCTGGA GCATCTCTTG ATAAAGTGAC CATGCAGCAG 
GTGGCCAGGA CAGTGGCTAA AGTGGAGCTC TCAGACCACG TGTGTGATGT GGTGTTTGCA 
CTCTTTGACT GTGATGGCAA TGGCGAACTG AGCAATAAGG AATTTGTTTC CATCATGAAG 
CAACGGCTGA TGAGAGGCCT GGAAAAGCCC AAAGACATGG GTTTCACTCG CCTCATGCAG 
GCCATGTGGA AATGTGCACA GGAAACTGCC TGGGACTTCG CTTTACCCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICU1 ( NM_001195519.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182448.1 Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22447
Clone ID Related Accession (Same CDS sequence)	NM_001195519.1
Accession Version	NM_001195519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	calcium uptake protein 1, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513185.12, AK296086.1 and BU627477.1. Transcript Variant: This variant (3) has multiple differences compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation to occur at a downstream start codon compared to variant 1. The encoded protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. In the absence of experimental data indicating otherwise, isoform 3 is presumed to be localized to the mitochondria, similarly to isoforms 1 and 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296086.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGAA TATATACTTA CAGACGGGCC AAGGAGATCT TCAAGGACAC TCCTAAAGCT 
CCTCAGAGAA ATTTTGAAAT TGCCTTCAAG ATGTTTGATT TGAATGGAGA TGGAGAAGTA 
GATATGGAAG AATTTGAACA GGTTCAGAGC ATCATTCGCT CCCAAACCAG TATGGGTATG 
CGCCACAGAG ATCGTCCAAC TACTGGCAAC ACCCTCAAGT CTGGCTTGTG TTCAGCCCTC 
ACAACCTACT TTTTTGGAGC TGATCTGAAG GGAAAGCTGA CAATCAAAAA CTTCCTCGAA 
TTTCAGCGTA AACTGCAGCA TGATGTTCTG AAGCTTGAGT TTGAACGCCA TGACCCTGTG 
GATGGGAGAA TTACTGAGAG GCAGTTTGGT GGCATGCTAC TTGCCTACAG TGGGGTGCAG 
TCCAAGAAGC TGACCGCCAT GCAGAGGCAG CTCAAGAAGC ACTTCAAAGA AGGAAAGGGT 
CTGACATTTC AGGAGGTGGA GAACTTCTTT ACTTTCCTAA AGAACATTAA TGATGTGGAC 
ACTGCATTGA GTTTTTACCA TATGGCTGGA GCATCTCTTG ATAAAGTGAC CATGCAGCAG 
GTGGCCAGGA CAGTGGCTAA AGTGGAGCTC TCAGACCACG TGTGTGATGT GGTGTTTGCA 
CTCTTTGACT GTGATGGCAA TGGCGAACTG AGCAATAAGG AATTTGTTTC CATCATGAAG 
CAACGGCTGA TGAGAGGCCT GGAAAAGCCC AAAGACATGG GTTTCACTCG CCTCATGCAG 
GCCATGTGGA AATGTGCACA GGAAACTGCC TGGGACTTCG CTTTACCCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182448.1
CDS	128..964
Translation	MKRIYTYRRAKEIFKDTPKAPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

Target ORF information:

RefSeq Version	NM_001195519.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195519.1

ORF Insert Sequence:

ATGAAAAGAA TATATACTTA CAGACGGGCC AAGGAGATCT TCAAGGACAC TCCTAAAGCT 
CCTCAGAGAA ATTTTGAAAT TGCCTTCAAG ATGTTTGATT TGAATGGAGA TGGAGAAGTA 
GATATGGAAG AATTTGAACA GGTTCAGAGC ATCATTCGCT CCCAAACCAG TATGGGTATG 
CGCCACAGAG ATCGTCCAAC TACTGGCAAC ACCCTCAAGT CTGGCTTGTG TTCAGCCCTC 
ACAACCTACT TTTTTGGAGC TGATCTGAAG GGAAAGCTGA CAATCAAAAA CTTCCTCGAA 
TTTCAGCGTA AACTGCAGCA TGATGTTCTG AAGCTTGAGT TTGAACGCCA TGACCCTGTG 
GATGGGAGAA TTACTGAGAG GCAGTTTGGT GGCATGCTAC TTGCCTACAG TGGGGTGCAG 
TCCAAGAAGC TGACCGCCAT GCAGAGGCAG CTCAAGAAGC ACTTCAAAGA AGGAAAGGGT 
CTGACATTTC AGGAGGTGGA GAACTTCTTT ACTTTCCTAA AGAACATTAA TGATGTGGAC 
ACTGCATTGA GTTTTTACCA TATGGCTGGA GCATCTCTTG ATAAAGTGAC CATGCAGCAG 
GTGGCCAGGA CAGTGGCTAA AGTGGAGCTC TCAGACCACG TGTGTGATGT GGTGTTTGCA 
CTCTTTGACT GTGATGGCAA TGGCGAACTG AGCAATAAGG AATTTGTTTC CATCATGAAG 
CAACGGCTGA TGAGAGGCCT GGAAAAGCCC AAAGACATGG GTTTCACTCG CCTCATGCAG 
GCCATGTGGA AATGTGCACA GGAAACTGCC TGGGACTTCG CTTTACCCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICU1 ( NM_001363513.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350442.1 Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu43919
Clone ID Related Accession (Same CDS sequence)	XM_005269383.2 , NM_001363513.1
Accession Version	NM_001363513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	calcium uptake protein 1, mitochondrial isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513185.12 and AC091769.6. On May 24, 2018 this sequence version replaced XM_005269383.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.24281.1, SRR1803614.127176.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGGAT 
TTCTGGCAGA CAGAGAAAAT TTCCCAGGAA CGAGAAAAAT TTGCTGATGA AGGCAGTATA 
TTTTACACCC TTGGAGAATG TGGGCTCATA TCCTTTTCAG ACTACATTTT CCTCACAACT 
GTTCTTTCCA CTCCTCAGAG AAATTTTGAA ATTGCCTTCA AGATGTTTGA TTTGAATGGA 
GATGGAGAAG TAGATATGGA AGAATTTGAA CAGGTTCAGA GCATCATTCG CTCCCAAACC 
AGTATGGGTA TGCGCCACAG AGATCGTCCA ACTACTGGCA ACACCCTCAA GTCTGGCTTG 
TGTTCAGCCC TCACAACCTA CTTTTTTGGA GCTGATCTGA AGGGAAAGCT GACAATCAAA 
AACTTCCTCG AATTTCAGCG TAAACTGCAG CATGATGTTC TGAAGCTTGA GTTTGAACGC 
CATGACCCTG TGGATGGGAG AATTACTGAG AGGCAGTTTG GTGGCATGCT ACTTGCCTAC 
AGTGGGGTGC AGTCCAAGAA GCTGACCGCC ATGCAGAGGC AGCTCAAGAA GCACTTCAAA 
GAAGGAAAGG GTCTGACATT TCAGGAGGTG GAGAACTTCT TTACTTTCCT AAAGAACATT 
AATGATGTGG ACACTGCATT GAGTTTTTAC CATATGGCTG GAGCATCTCT TGATAAAGTG 
ACCATGCAGC AGGTGGCCAG GACAGTGGCT AAAGTGGAGC TCTCAGACCA CGTGTGTGAT 
GTGGTGTTTG CACTCTTTGA CTGTGATGGC AATGGCGAAC TGAGCAATAA GGAATTTGTT 
TCCATCATGA AGCAACGGCT GATGAGAGGC CTGGAAAAGC CCAAAGACAT GGGTTTCACT 
CGCCTCATGC AGGCCATGTG GAAATGTGCA CAGGAAACTG CCTGGGACTT CGCTTTACCC 
AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350442.1
CDS	184..1632
Translation	MFRLNSLSALAELAVGSRWYHGGSQPIQIRRRLMMVAFLGASAVTASTGLLWKRAHAESPPCVDNLKSDIGDKGKNKDEGDVCNHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKDFWQTEKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

Target ORF information:

RefSeq Version	NM_001363513.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363513.1

ORF Insert Sequence:

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGGAT 
TTCTGGCAGA CAGAGAAAAT TTCCCAGGAA CGAGAAAAAT TTGCTGATGA AGGCAGTATA 
TTTTACACCC TTGGAGAATG TGGGCTCATA TCCTTTTCAG ACTACATTTT CCTCACAACT 
GTTCTTTCCA CTCCTCAGAG AAATTTTGAA ATTGCCTTCA AGATGTTTGA TTTGAATGGA 
GATGGAGAAG TAGATATGGA AGAATTTGAA CAGGTTCAGA GCATCATTCG CTCCCAAACC 
AGTATGGGTA TGCGCCACAG AGATCGTCCA ACTACTGGCA ACACCCTCAA GTCTGGCTTG 
TGTTCAGCCC TCACAACCTA CTTTTTTGGA GCTGATCTGA AGGGAAAGCT GACAATCAAA 
AACTTCCTCG AATTTCAGCG TAAACTGCAG CATGATGTTC TGAAGCTTGA GTTTGAACGC 
CATGACCCTG TGGATGGGAG AATTACTGAG AGGCAGTTTG GTGGCATGCT ACTTGCCTAC 
AGTGGGGTGC AGTCCAAGAA GCTGACCGCC ATGCAGAGGC AGCTCAAGAA GCACTTCAAA 
GAAGGAAAGG GTCTGACATT TCAGGAGGTG GAGAACTTCT TTACTTTCCT AAAGAACATT 
AATGATGTGG ACACTGCATT GAGTTTTTAC CATATGGCTG GAGCATCTCT TGATAAAGTG 
ACCATGCAGC AGGTGGCCAG GACAGTGGCT AAAGTGGAGC TCTCAGACCA CGTGTGTGAT 
GTGGTGTTTG CACTCTTTGA CTGTGATGGC AATGGCGAAC TGAGCAATAA GGAATTTGTT 
TCCATCATGA AGCAACGGCT GATGAGAGGC CTGGAAAAGC CCAAAGACAT GGGTTTCACT 
CGCCTCATGC AGGCCATGTG GAAATGTGCA CAGGAAACTG CCTGGGACTT CGCTTTACCC 
AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICU1 ( XM_005269383.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269440.1 Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X2, mRNA.

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CloneID	OHu43919
Clone ID Related Accession (Same CDS sequence)	XM_005269383.2 , NM_001363513.1
Accession Version	XM_005269383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	calcium uptake protein 1, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005269383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGGAT 
TTCTGGCAGA CAGAGAAAAT TTCCCAGGAA CGAGAAAAAT TTGCTGATGA AGGCAGTATA 
TTTTACACCC TTGGAGAATG TGGGCTCATA TCCTTTTCAG ACTACATTTT CCTCACAACT 
GTTCTTTCCA CTCCTCAGAG AAATTTTGAA ATTGCCTTCA AGATGTTTGA TTTGAATGGA 
GATGGAGAAG TAGATATGGA AGAATTTGAA CAGGTTCAGA GCATCATTCG CTCCCAAACC 
AGTATGGGTA TGCGCCACAG AGATCGTCCA ACTACTGGCA ACACCCTCAA GTCTGGCTTG 
TGTTCAGCCC TCACAACCTA CTTTTTTGGA GCTGATCTGA AGGGAAAGCT GACAATCAAA 
AACTTCCTCG AATTTCAGCG TAAACTGCAG CATGATGTTC TGAAGCTTGA GTTTGAACGC 
CATGACCCTG TGGATGGGAG AATTACTGAG AGGCAGTTTG GTGGCATGCT ACTTGCCTAC 
AGTGGGGTGC AGTCCAAGAA GCTGACCGCC ATGCAGAGGC AGCTCAAGAA GCACTTCAAA 
GAAGGAAAGG GTCTGACATT TCAGGAGGTG GAGAACTTCT TTACTTTCCT AAAGAACATT 
AATGATGTGG ACACTGCATT GAGTTTTTAC CATATGGCTG GAGCATCTCT TGATAAAGTG 
ACCATGCAGC AGGTGGCCAG GACAGTGGCT AAAGTGGAGC TCTCAGACCA CGTGTGTGAT 
GTGGTGTTTG CACTCTTTGA CTGTGATGGC AATGGCGAAC TGAGCAATAA GGAATTTGTT 
TCCATCATGA AGCAACGGCT GATGAGAGGC CTGGAAAAGC CCAAAGACAT GGGTTTCACT 
CGCCTCATGC AGGCCATGTG GAAATGTGCA CAGGAAACTG CCTGGGACTT CGCTTTACCC 
AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269440.1
CDS	88..1536
Translation	MFRLNSLSALAELAVGSRWYHGGSQPIQIRRRLMMVAFLGASAVTASTGLLWKRAHAESPPCVDNLKSDIGDKGKNKDEGDVCNHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKDFWQTEKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

Target ORF information:

RefSeq Version	XM_005269383.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269383.2

ORF Insert Sequence:

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGGAT 
TTCTGGCAGA CAGAGAAAAT TTCCCAGGAA CGAGAAAAAT TTGCTGATGA AGGCAGTATA 
TTTTACACCC TTGGAGAATG TGGGCTCATA TCCTTTTCAG ACTACATTTT CCTCACAACT 
GTTCTTTCCA CTCCTCAGAG AAATTTTGAA ATTGCCTTCA AGATGTTTGA TTTGAATGGA 
GATGGAGAAG TAGATATGGA AGAATTTGAA CAGGTTCAGA GCATCATTCG CTCCCAAACC 
AGTATGGGTA TGCGCCACAG AGATCGTCCA ACTACTGGCA ACACCCTCAA GTCTGGCTTG 
TGTTCAGCCC TCACAACCTA CTTTTTTGGA GCTGATCTGA AGGGAAAGCT GACAATCAAA 
AACTTCCTCG AATTTCAGCG TAAACTGCAG CATGATGTTC TGAAGCTTGA GTTTGAACGC 
CATGACCCTG TGGATGGGAG AATTACTGAG AGGCAGTTTG GTGGCATGCT ACTTGCCTAC 
AGTGGGGTGC AGTCCAAGAA GCTGACCGCC ATGCAGAGGC AGCTCAAGAA GCACTTCAAA 
GAAGGAAAGG GTCTGACATT TCAGGAGGTG GAGAACTTCT TTACTTTCCT AAAGAACATT 
AATGATGTGG ACACTGCATT GAGTTTTTAC CATATGGCTG GAGCATCTCT TGATAAAGTG 
ACCATGCAGC AGGTGGCCAG GACAGTGGCT AAAGTGGAGC TCTCAGACCA CGTGTGTGAT 
GTGGTGTTTG CACTCTTTGA CTGTGATGGC AATGGCGAAC TGAGCAATAA GGAATTTGTT 
TCCATCATGA AGCAACGGCT GATGAGAGGC CTGGAAAAGC CCAAAGACAT GGGTTTCACT 
CGCCTCATGC AGGCCATGTG GAAATGTGCA CAGGAAACTG CCTGGGACTT CGCTTTACCC 
AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICU1 ( XM_005269386.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269443.1 Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X6, mRNA.

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CloneID	OHu43921
Clone ID Related Accession (Same CDS sequence)	XM_005269386.2
Accession Version	XM_005269386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	calcium uptake protein 1, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005269386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGATT TGAATGGAGA TGGAGAAGTA GATATGGAAG AATTTGAACA GGTTCAGAGC 
ATCATTCGCT CCCAAACCAG TATGGGTATG CGCCACAGAG ATCGTCCAAC TACTGGCAAC 
ACCCTCAAGT CTGGCTTGTG TTCAGCCCTC ACAACCTACT TTTTTGGAGC TGATCTGAAG 
GGAAAGCTGA CAATCAAAAA CTTCCTCGAA TTTCAGCGTA AACTGCAGCA TGATGTTCTG 
AAGCTTGAGT TTGAACGCCA TGACCCTGTG GATGGGAGAA TTACTGAGAG GCAGTTTGGT 
GGCATGCTAC TTGCCTACAG TGGGGTGCAG TCCAAGAAGC TGACCGCCAT GCAGAGGCAG 
CTCAAGAAGC ACTTCAAAGA AGGAAAGGGT CTGACATTTC AGGAGGTGGA GAACTTCTTT 
ACTTTCCTAA AGAACATTAA TGATGTGGAC ACTGCATTGA GTTTTTACCA TATGGCTGGA 
GCATCTCTTG ATAAAGTGAC CATGCAGCAG GTGGCCAGGA CAGTGGCTAA AGTGGAGCTC 
TCAGACCACG TGTGTGATGT GGTGTTTGCA CTCTTTGACT GTGATGGCAA TGGCGAACTG 
AGCAATAAGG AATTTGTTTC CATCATGAAG CAACGGCTGA TGAGAGGCCT GGAAAAGCCC 
AAAGACATGG GTTTCACTCG CCTCATGCAG GCCATGTGGA AATGTGCACA GGAAACTGCC 
TGGGACTTCG CTTTACCCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269443.1
CDS	331..1077
Translation	MFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

Target ORF information:

RefSeq Version	XM_005269386.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269386.2

ORF Insert Sequence:

ATGTTTGATT TGAATGGAGA TGGAGAAGTA GATATGGAAG AATTTGAACA GGTTCAGAGC 
ATCATTCGCT CCCAAACCAG TATGGGTATG CGCCACAGAG ATCGTCCAAC TACTGGCAAC 
ACCCTCAAGT CTGGCTTGTG TTCAGCCCTC ACAACCTACT TTTTTGGAGC TGATCTGAAG 
GGAAAGCTGA CAATCAAAAA CTTCCTCGAA TTTCAGCGTA AACTGCAGCA TGATGTTCTG 
AAGCTTGAGT TTGAACGCCA TGACCCTGTG GATGGGAGAA TTACTGAGAG GCAGTTTGGT 
GGCATGCTAC TTGCCTACAG TGGGGTGCAG TCCAAGAAGC TGACCGCCAT GCAGAGGCAG 
CTCAAGAAGC ACTTCAAAGA AGGAAAGGGT CTGACATTTC AGGAGGTGGA GAACTTCTTT 
ACTTTCCTAA AGAACATTAA TGATGTGGAC ACTGCATTGA GTTTTTACCA TATGGCTGGA 
GCATCTCTTG ATAAAGTGAC CATGCAGCAG GTGGCCAGGA CAGTGGCTAA AGTGGAGCTC 
TCAGACCACG TGTGTGATGT GGTGTTTGCA CTCTTTGACT GTGATGGCAA TGGCGAACTG 
AGCAATAAGG AATTTGTTTC CATCATGAAG CAACGGCTGA TGAGAGGCCT GGAAAAGCCC 
AAAGACATGG GTTTCACTCG CCTCATGCAG GCCATGTGGA AATGTGCACA GGAAACTGCC 
TGGGACTTCG CTTTACCCAA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICU1 ( XM_011539119.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537421.1 Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X1, mRNA.

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CloneID	OHu63947
Clone ID Related Accession (Same CDS sequence)	XM_011539119.1
Accession Version	XM_011539119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	calcium uptake protein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGGCC 
AGATTCTCCA AAAAGAAAAT ATTACACAGG GTCAGAAAGA AAAGTAAAGG GTCAATTTTA 
CAGAGGAGGA AGAAAGCACT TCAGAAAGAA GAAAGAACCA GGCATGGTTC TACAAAGCAC 
AATGAACAGG AAGTCAGAAG GACAATCATG GAGTGGCTGG AAAAGAAAAT TTCCCAGGAA 
CGAGAAAAAT TTGCTGATGA AGGCAGTATA TTTTACACCC TTGGAGAATG TGGGCTCATA 
TCCTTTTCAG ACTACATTTT CCTCACAACT GTTCTTTCCA CTCCTCAGAG AAATTTTGAA 
ATTGCCTTCA AGATGTTTGA TTTGAATGGA GATGGAGAAG TAGATATGGA AGAATTTGAA 
CAGGTTCAGA GCATCATTCG CTCCCAAACC AGTATGGGTA TGCGCCACAG AGATCGTCCA 
ACTACTGGCA ACACCCTCAA GTCTGGCTTG TGTTCAGCCC TCACAACCTA CTTTTTTGGA 
GCTGATCTGA AGGGAAAGCT GACAATCAAA AACTTCCTCG AATTTCAGCG TAAACTGCAG 
CATGATGTTC TGAAGCTTGA GTTTGAACGC CATGACCCTG TGGATGGGAG AATTACTGAG 
AGGCAGTTTG GTGGCATGCT ACTTGCCTAC AGTGGGGTGC AGTCCAAGAA GCTGACCGCC 
ATGCAGAGGC AGCTCAAGAA GCACTTCAAA GAAGGAAAGG GTCTGACATT TCAGGAGGTG 
GAGAACTTCT TTACTTTCCT AAAGAACATT AATGATGTGG ACACTGCATT GAGTTTTTAC 
CATATGGCTG GAGCATCTCT TGATAAAGTG ACCATGCAGC AGGTGGCCAG GACAGTGGCT 
AAAGTGGAGC TCTCAGACCA CGTGTGTGAT GTGGTGTTTG CACTCTTTGA CTGTGATGGC 
AATGGCGAAC TGAGCAATAA GGAATTTGTT TCCATCATGA AGCAACGGCT GATGAGAGGC 
CTGGAAAAGC CCAAAGACAT GGGTTTCACT CGCCTCATGC AGGCCATGTG GAAATGTGCA 
CAGGAAACTG CCTGGGACTT CGCTTTACCC AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537421.1
CDS	88..1686
Translation	MFRLNSLSALAELAVGSRWYHGGSQPIQIRRRLMMVAFLGASAVTASTGLLWKRAHAESPPCVDNLKSDIGDKGKNKDEGDVCNHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKARFSKKKILHRVRKKSKGSILQRRKKALQKEERTRHGSTKHNEQEVRRTIMEWLEKKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

Target ORF information:

RefSeq Version	XM_011539119.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539119.1

ORF Insert Sequence:

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGGCC 
AGATTCTCCA AAAAGAAAAT ATTACACAGG GTCAGAAAGA AAAGTAAAGG GTCAATTTTA 
CAGAGGAGGA AGAAAGCACT TCAGAAAGAA GAAAGAACCA GGCATGGTTC TACAAAGCAC 
AATGAACAGG AAGTCAGAAG GACAATCATG GAGTGGCTGG AAAAGAAAAT TTCCCAGGAA 
CGAGAAAAAT TTGCTGATGA AGGCAGTATA TTTTACACCC TTGGAGAATG TGGGCTCATA 
TCCTTTTCAG ACTACATTTT CCTCACAACT GTTCTTTCCA CTCCTCAGAG AAATTTTGAA 
ATTGCCTTCA AGATGTTTGA TTTGAATGGA GATGGAGAAG TAGATATGGA AGAATTTGAA 
CAGGTTCAGA GCATCATTCG CTCCCAAACC AGTATGGGTA TGCGCCACAG AGATCGTCCA 
ACTACTGGCA ACACCCTCAA GTCTGGCTTG TGTTCAGCCC TCACAACCTA CTTTTTTGGA 
GCTGATCTGA AGGGAAAGCT GACAATCAAA AACTTCCTCG AATTTCAGCG TAAACTGCAG 
CATGATGTTC TGAAGCTTGA GTTTGAACGC CATGACCCTG TGGATGGGAG AATTACTGAG 
AGGCAGTTTG GTGGCATGCT ACTTGCCTAC AGTGGGGTGC AGTCCAAGAA GCTGACCGCC 
ATGCAGAGGC AGCTCAAGAA GCACTTCAAA GAAGGAAAGG GTCTGACATT TCAGGAGGTG 
GAGAACTTCT TTACTTTCCT AAAGAACATT AATGATGTGG ACACTGCATT GAGTTTTTAC 
CATATGGCTG GAGCATCTCT TGATAAAGTG ACCATGCAGC AGGTGGCCAG GACAGTGGCT 
AAAGTGGAGC TCTCAGACCA CGTGTGTGAT GTGGTGTTTG CACTCTTTGA CTGTGATGGC 
AATGGCGAAC TGAGCAATAA GGAATTTGTT TCCATCATGA AGCAACGGCT GATGAGAGGC 
CTGGAAAAGC CCAAAGACAT GGGTTTCACT CGCCTCATGC AGGCCATGTG GAAATGTGCA 
CAGGAAACTG CCTGGGACTT CGCTTTACCC AAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICU1 ( NM_001195518.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182447.1 Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22187
Clone ID Related Accession (Same CDS sequence)	NM_001195518.2 , NM_001195518.1
Accession Version	NM_001195518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	calcium uptake protein 1, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK023318.1, AL513185.12 and AC091769.6. On Nov 22, 2018 this sequence version replaced NM_001195518.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004190.2, AK225298.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000361114.10/ ENSP00000354415.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGAAA 
ATTTCCCAGG AACGAGAAAA ATTTGCTGAT GAAGGCAGTA TATTTTACAC CCTTGGAGAA 
TGTGGGCTCA TATCCTTTTC AGACTACATT TTCCTCACAA CTGTTCTTTC CACTCCTCAG 
AGAAATTTTG AAATTGCCTT CAAGATGTTT GATTTGAATG GAGATGGAGA AGTAGATATG 
GAAGAATTTG AACAGGTTCA GAGCATCATT CGCTCCCAAA CCAGTATGGG TATGCGCCAC 
AGAGATCGTC CAACTACTGG CAACACCCTC AAGTCTGGCT TGTGTTCAGC CCTCACAACC 
TACTTTTTTG GAGCTGATCT GAAGGGAAAG CTGACAATCA AAAACTTCCT CGAATTTCAG 
CGTAAACTGC AGCATGATGT TCTGAAGCTT GAGTTTGAAC GCCATGACCC TGTGGATGGG 
AGAATTACTG AGAGGCAGTT TGGTGGCATG CTACTTGCCT ACAGTGGGGT GCAGTCCAAG 
AAGCTGACCG CCATGCAGAG GCAGCTCAAG AAGCACTTCA AAGAAGGAAA GGGTCTGACA 
TTTCAGGAGG TGGAGAACTT CTTTACTTTC CTAAAGAACA TTAATGATGT GGACACTGCA 
TTGAGTTTTT ACCATATGGC TGGAGCATCT CTTGATAAAG TGACCATGCA GCAGGTGGCC 
AGGACAGTGG CTAAAGTGGA GCTCTCAGAC CACGTGTGTG ATGTGGTGTT TGCACTCTTT 
GACTGTGATG GCAATGGCGA ACTGAGCAAT AAGGAATTTG TTTCCATCAT GAAGCAACGG 
CTGATGAGAG GCCTGGAAAA GCCCAAAGAC ATGGGTTTCA CTCGCCTCAT GCAGGCCATG 
TGGAAATGTG CACAGGAAAC TGCCTGGGAC TTCGCTTTAC CCAAACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182447.1
CDS	72..1502
Translation	MFRLNSLSALAELAVGSRWYHGGSQPIQIRRRLMMVAFLGASAVTASTGLLWKRAHAESPPCVDNLKSDIGDKGKNKDEGDVCNHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

Target ORF information:

RefSeq Version	NM_001195518.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195518.2

ORF Insert Sequence:

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGAAA 
ATTTCCCAGG AACGAGAAAA ATTTGCTGAT GAAGGCAGTA TATTTTACAC CCTTGGAGAA 
TGTGGGCTCA TATCCTTTTC AGACTACATT TTCCTCACAA CTGTTCTTTC CACTCCTCAG 
AGAAATTTTG AAATTGCCTT CAAGATGTTT GATTTGAATG GAGATGGAGA AGTAGATATG 
GAAGAATTTG AACAGGTTCA GAGCATCATT CGCTCCCAAA CCAGTATGGG TATGCGCCAC 
AGAGATCGTC CAACTACTGG CAACACCCTC AAGTCTGGCT TGTGTTCAGC CCTCACAACC 
TACTTTTTTG GAGCTGATCT GAAGGGAAAG CTGACAATCA AAAACTTCCT CGAATTTCAG 
CGTAAACTGC AGCATGATGT TCTGAAGCTT GAGTTTGAAC GCCATGACCC TGTGGATGGG 
AGAATTACTG AGAGGCAGTT TGGTGGCATG CTACTTGCCT ACAGTGGGGT GCAGTCCAAG 
AAGCTGACCG CCATGCAGAG GCAGCTCAAG AAGCACTTCA AAGAAGGAAA GGGTCTGACA 
TTTCAGGAGG TGGAGAACTT CTTTACTTTC CTAAAGAACA TTAATGATGT GGACACTGCA 
TTGAGTTTTT ACCATATGGC TGGAGCATCT CTTGATAAAG TGACCATGCA GCAGGTGGCC 
AGGACAGTGG CTAAAGTGGA GCTCTCAGAC CACGTGTGTG ATGTGGTGTT TGCACTCTTT 
GACTGTGATG GCAATGGCGA ACTGAGCAAT AAGGAATTTG TTTCCATCAT GAAGCAACGG 
CTGATGAGAG GCCTGGAAAA GCCCAAAGAC ATGGGTTTCA CTCGCCTCAT GCAGGCCATG 
TGGAAATGTG CACAGGAAAC TGCCTGGGAC TTCGCTTTAC CCAAACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICU1 ( NM_001195518.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182447.1 Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 2, mRNA.

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CloneID	OHu22187
Clone ID Related Accession (Same CDS sequence)	NM_001195518.2 , NM_001195518.1
Accession Version	NM_001195518.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Homo sapiens(human)
Product	calcium uptake protein 1, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513185.12, AK023318.1, AC091769.6 and BU627477.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.208703.1, SRR1803616.260586.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGAAA 
ATTTCCCAGG AACGAGAAAA ATTTGCTGAT GAAGGCAGTA TATTTTACAC CCTTGGAGAA 
TGTGGGCTCA TATCCTTTTC AGACTACATT TTCCTCACAA CTGTTCTTTC CACTCCTCAG 
AGAAATTTTG AAATTGCCTT CAAGATGTTT GATTTGAATG GAGATGGAGA AGTAGATATG 
GAAGAATTTG AACAGGTTCA GAGCATCATT CGCTCCCAAA CCAGTATGGG TATGCGCCAC 
AGAGATCGTC CAACTACTGG CAACACCCTC AAGTCTGGCT TGTGTTCAGC CCTCACAACC 
TACTTTTTTG GAGCTGATCT GAAGGGAAAG CTGACAATCA AAAACTTCCT CGAATTTCAG 
CGTAAACTGC AGCATGATGT TCTGAAGCTT GAGTTTGAAC GCCATGACCC TGTGGATGGG 
AGAATTACTG AGAGGCAGTT TGGTGGCATG CTACTTGCCT ACAGTGGGGT GCAGTCCAAG 
AAGCTGACCG CCATGCAGAG GCAGCTCAAG AAGCACTTCA AAGAAGGAAA GGGTCTGACA 
TTTCAGGAGG TGGAGAACTT CTTTACTTTC CTAAAGAACA TTAATGATGT GGACACTGCA 
TTGAGTTTTT ACCATATGGC TGGAGCATCT CTTGATAAAG TGACCATGCA GCAGGTGGCC 
AGGACAGTGG CTAAAGTGGA GCTCTCAGAC CACGTGTGTG ATGTGGTGTT TGCACTCTTT 
GACTGTGATG GCAATGGCGA ACTGAGCAAT AAGGAATTTG TTTCCATCAT GAAGCAACGG 
CTGATGAGAG GCCTGGAAAA GCCCAAAGAC ATGGGTTTCA CTCGCCTCAT GCAGGCCATG 
TGGAAATGTG CACAGGAAAC TGCCTGGGAC TTCGCTTTAC CCAAACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182447.1
CDS	184..1614
Translation	MFRLNSLSALAELAVGSRWYHGGSQPIQIRRRLMMVAFLGASAVTASTGLLWKRAHAESPPCVDNLKSDIGDKGKNKDEGDVCNHEKKTADLAPHPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLMRGLEKPKDMGFTRLMQAMWKCAQETAWDFALPKQ

Target ORF information:

RefSeq Version	NM_001195518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial calcium uptake 1 (MICU1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195518.1

ORF Insert Sequence:

ATGTTTCGTC TGAACTCACT TTCTGCTTTG GCAGAACTGG CTGTGGGTTC TCGATGGTAC 
CATGGAGGAT CACAGCCCAT CCAGATCCGG CGAAGACTAA TGATGGTGGC TTTCCTGGGA 
GCATCTGCAG TAACTGCAAG TACTGGTCTT TTGTGGAAGA GGGCCCATGC AGAATCTCCA 
CCATGTGTAG ACAACCTAAA AAGTGACATC GGTGATAAAG GGAAGAATAA AGATGAAGGG 
GATGTTTGTA ACCATGAGAA AAAGACTGCA GATCTTGCCC CTCACCCAGA AGAGAAAAAG 
AAGAAACGTT CTGGATTCAG AGACAGAAAA GTGATGGAAT ATGAGAATAG GATTCGAGCC 
TACTCCACGC CAGACAAAAT CTTCCGATAT TTTGCCACCT TGAAAGTCAT CAGTGAGCCT 
GGTGAAGCAG AAGTGTTTAT GACACCAGAA GATTTTGTGC GATCCATAAC ACCCAATGAA 
AAACAACCAG AACACTTGGG TCTGGATCAA TATATAATAA AACGCTTTGA TGGAAAGAAA 
ATTTCCCAGG AACGAGAAAA ATTTGCTGAT GAAGGCAGTA TATTTTACAC CCTTGGAGAA 
TGTGGGCTCA TATCCTTTTC AGACTACATT TTCCTCACAA CTGTTCTTTC CACTCCTCAG 
AGAAATTTTG AAATTGCCTT CAAGATGTTT GATTTGAATG GAGATGGAGA AGTAGATATG 
GAAGAATTTG AACAGGTTCA GAGCATCATT CGCTCCCAAA CCAGTATGGG TATGCGCCAC 
AGAGATCGTC CAACTACTGG CAACACCCTC AAGTCTGGCT TGTGTTCAGC CCTCACAACC 
TACTTTTTTG GAGCTGATCT GAAGGGAAAG CTGACAATCA AAAACTTCCT CGAATTTCAG 
CGTAAACTGC AGCATGATGT TCTGAAGCTT GAGTTTGAAC GCCATGACCC TGTGGATGGG 
AGAATTACTG AGAGGCAGTT TGGTGGCATG CTACTTGCCT ACAGTGGGGT GCAGTCCAAG 
AAGCTGACCG CCATGCAGAG GCAGCTCAAG AAGCACTTCA AAGAAGGAAA GGGTCTGACA 
TTTCAGGAGG TGGAGAACTT CTTTACTTTC CTAAAGAACA TTAATGATGT GGACACTGCA 
TTGAGTTTTT ACCATATGGC TGGAGCATCT CTTGATAAAG TGACCATGCA GCAGGTGGCC 
AGGACAGTGG CTAAAGTGGA GCTCTCAGAC CACGTGTGTG ATGTGGTGTT TGCACTCTTT 
GACTGTGATG GCAATGGCGA ACTGAGCAAT AAGGAATTTG TTTCCATCAT GAAGCAACGG 
CTGATGAGAG GCCTGGAAAA GCCCAAAGAC ATGGGTTTCA CTCGCCTCAT GCAGGCCATG 
TGGAAATGTG CACAGGAAAC TGCCTGGGAC TTCGCTTTAC CCAAACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MICU1 controls cristae junction and spatially anchors mitochondrial Ca
Nature communications10(1)3732(2019 08)
Gottschalk B,Klec C,Leitinger G,Bernhart E,Rost R,Bischof H,Madreiter-Sokolowski CT,Radulović S,Eroglu E,Sattler W,Waldeck-Weiermair M,Malli R,Graier WF

Akt-mediated phosphorylation of MICU1 regulates mitochondrial Ca
The EMBO journal38(2)(2019 01)
Marchi S,Corricelli M,Branchini A,Vitto VAM,Missiroli S,Morciano G,Perrone M,Ferrarese M,Giorgi C,Pinotti M,Galluzzi L,Kroemer G,Pinton P

MICU1 Confers Protection from MCU-Dependent Manganese Toxicity.
Cell reports25(6)1425-1435.e7(2018 11)
Wettmarshausen J,Goh V,Huang KT,Arduino DM,Tripathi U,Leimpek A,Cheng Y,Pittis AA,Gabaldón T,Mokranjac D,Hajnóczky G,Perocchi F

MICU1 Interacts with the D-Ring of the MCU Pore to Control Its Ca
Molecular cell72(4)778-785.e3(2018 11)
Paillard M,Csordás G,Huang KT,Várnai P,Joseph SK,Hajnóczky G

Pathological consequences of MICU1 mutations on mitochondrial calcium signalling and bioenergetics.
Biochimica et biophysica acta. Molecular cell research1864(6)1009-1017(2017 Jun)
Bhosale G,Sharpe JA,Koh A,Kouli A,Szabadkai G,Duchen MR

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MICU1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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