ANO8 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANO8
Entrez Gene ID	429912
Full Name	anoctamin 8
Synonyms	TMEM16H
General protein information	Preferred Names anoctamin 8 Names anoctamin-8 transmembrane protein 16H
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025144391.1	XP_025000159.1	anoctamin-8
XM_015300106.1	XP_015155592.1	anoctamin-8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ano8	XP_006253027.1
Gallus gallus (chicken)	ANO8	XP_427467.4
Mus musculus (house mouse)	Ano8	NP_001158151.1
Xenopus tropicalis (tropical clawed frog)	ano8	XP_004911073.1
Caenorhabditis elegans (roundworm)	anoh-2	NP_001255820.1
Canis lupus familiaris (dog)	ANO8	XP_003639823.1
Bos taurus (cattle)	ANO8	XP_002688590.1
Homo sapiens (human)	ANO8	NP_066010.1
Pan troglodytes (chimpanzee)	ANO8	XP_003953455.1
Danio rerio (zebrafish)	ano8	XP_691248.6

Gene Ontology

The following ANO8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa148467	XM_025144391.1 Latest version!	Gallus gallus anoctamin 8 (ANO8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa47361	XM_015300106.1 Latest version!	Gallus gallus anoctamin 8 (ANO8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANO8 ( XM_025144391.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025000159.1 Gallus gallus anoctamin 8 (ANO8), mRNA.

Price & Availability↑

CloneID	OGa148467
Clone ID Related Accession (Same CDS sequence)	XM_025144391.1
Accession Version	XM_025144391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	anoctamin-8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006115.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC CGCCCGCGGC ACCGCACGAC GGAGAGCGGC CCCGGAGGGC CCCGCCTGGG 
GACGAACGGA CGGAGCCGGC GGCCCCGGGG GGGGTCCTGG ACAAGCTTTT CGGGAAGCGG 
CTGCTCCAAG CTGGGCGCTA CATCATGTCC CACAAAGCCT GGATGAAGAC GGTGCCCACC 
GAGAACTGTG ATGTGCTCAT GACCTTCCCA GACACCACGG ATGACCACAC GCTGCTTTGG 
CTGCTGAACC ACATCCGCCT CGGCATCCCC GAGCTCATCG TGCAGGTGCG GCACCACAAG 
CACACCCGCG CCTACGCCTT CTTCGTCACC GCCACCTACG AGAGTTTGCT GCGTGGGGCC 
GATGAGATCG GGCTGCGGAA ACCGGTGAAG GCTGAGTTTG GGGGCGGCAT GCGGAGCTTC 
TCCTGCGAGG AGGACTACAT CTATGAGAAC ATTGAGAACG AGCTTTACTT CTTCACCTCT 
CAGGAACGGC AAAACATCAT CAGGTATTGG CTGGAGAACC TGCGGGCCAA GCAGGGCGAG 
GCGCTGCACA ACATCCACTT CCTGGAGGGA CAGCCCATCA TCCCGGAGCT GGCGGCCCGC 
GGCGTCATCC AGCAGGTCTT CCCACTGCAC GAGCAGAGGA TCCTCAAGCG GCTGATGAAG 
TCGTGGGTGC AGGCGATCTG CGAGGCGCAG CCACTCGATG AGATCTGTGA CTACTTCGGG 
GTGAAGATCG CCATGTACTT CGCCTGGCTC GGCTTCTACA CCTCGGCCAT GGTGTACCCT 
GCTGTGTTCG GCTCCATCCT CTACACCTTC ACTGAGAGCG ACCAGACCAG CCAGGACATC 
TGCTGTGTGG TGTTTGCCAT CTTCAACGTC GTCTGGGCCA CGCTGTTCCT GGAGGAGTGG 
AAGCGCCGCG GGGCAGAGTT TGCCTACAAG TGGGGGACGC TGGACACCCC CGCAGAGTCC 
ATCGAGGAGC CCCGGCCCCA GTTCAGGGGG GTGAAGAGGA TCAGCCCGGT GACCAGCGCC 
GAGGAGTTCT ACTACCCGCC CTGGAAGCGG CTGCTGTTCC AGTGCCTGGT CAGCCTGCCC 
GTCTGCCTCG CCTGCCTCTC CTTCGTCTTC CTCGTCATGC TGGGCTGCTT CCAGCTCCAG 
GAGCTCGTGC TGAGCGTCAA GGAGCTGCCC CGCATCCTCC GCTTCCTGCC CAAAATCGTG 
CTGGCCGTCA TCGTCACGAC CTGCGACGAG GTTTACAAGA AGATCGCCTA CTGGCTGAAT 
GATATGGAAA ACTACCGCCT GCAGAGTGCC TACGAGAAAC ACCTCATCAT CAAAATCGTC 
CTGTTTCAGT TTGTAAATTC ATACCTAAGT CTTTTCTACA TCGGTTTCTA CCTCAAAGAC 
ATGGAGCGGC TGAAGGAGAT GCTGGCCACG CTGCTCATCA CACGGCAGTT CCTGCAGAAC 
GTCAAGGAGG TGTCGCAGCC CCACCTGTAC CGCCGCCTGC GCCGGGGGGA GCTCAGCCTG 
CGCAACCTCC GCGAGCTGTC CCACGCCGTC CTCCGCCTGC TCGCCCACCC CCGCGCACCC 
CCGGCCACTG GAGCAGCCCA CGAGGGGCCG CGGGGGGAGA AGAAGTGTCT GAACGGGGGC 
TGCGGGGTGC CCGAGGAGGA GGAGGAGGAG GAAGAGCGTC GGGAGTCAGA TTCGGAGGAT 
GAGAGCGTGC TGGACTGTGG GCTGAAGCTG AAGAAGGTGA GCTTCATCGA GAAGGCGGAG 
CGGCGCGGCA CCGAGCCCTG CAGCACCGAG GAGGAGAGCT TCCTGGAGGA GGGCAGCCCC 
ACCATGGTGG AGAAGGGAAT GGACCCCGCC TCCGTCTTCG AGCTGGGTGA CGATGAGGAT 
GATGCTGAAG GCCCCTCAGG CAGCCCAACC AAGGCAGCGG AGCCGGCCAC AGGCCCACGG 
GTCACCCGGA GGAGGAGGGA GGATGAGGAG GGTGAGGAGG AAGGGCGCAG GCGGAACCGG 
GCATCATGGA TCGATCCGCC CGAGGAGGAC TACTCGACAC AGCTGACGCA GGCGGAGGTG 
GAGAGCTGCA TGAAGAAGTA CGAGGACACC TTCCAGGACT ACCAGGAGAT GTTCATCCAG 
TTTGGCTACG TGGTGCTCTT CTCCTCTGCC TTCCCACTGG CCGCCATGTG TGCGCTGGTC 
AACAACATCA TCGAGATTCG CAGTGATGCC TTCAAACTGT GCACGGGGCT GCAGCGGCCC 
TTTGGGCAGC GAGTGGAGAG CATTGGGCAG TGGCAGAAGG TGATGGAGGC CATGGGCGTG 
TTGGCCATCG TGGTCAACTG CTACCTGATC GCGCAGTGCG GGCAGCTGCA GCGGCTCTTC 
CCTTGGCTCA GCCCCGAGGG AGCCATCATC TCGGTGGTGG TGCTGGAGCA CTTCGCCCTG 
CTGCTGAAGT ACGTCATCCA AGTGGCCATC CCTGACATCC CTGCGTGGGT GGCCGAGGAG 
ATGGCCAAGC TGGAGTACCA GCGCCGCGAG GCCTTCAAGA AGCACGAGCG CCAGGCCCAG 
CACCACTTCC AGCAGCAGCA GCGGCGCAAG CGGGAGGAGG AGGAGCGGCA GCGGCACGCC 
GAGTACCAGG CGCGCAAGGA GCGCGAGGCC AACCGCGACG AGGCCAAGGC TGAGGCTGCC 
GGGCAGGACC CGGCCCACGA CAAGAGCCAG AGCAAAGGGA AGGGCTCCGG GGGGTCCTCC 
GCGCACGGCT CCGACAAGCC CAAGCGGCCC AGCTCGCTGC TGGCCACCAA TAACGTCATG 
AAGCTGAAGC AGATCATCCC TCTGCAGGGC AAATTCCTCT CCGGGGGGGC CGGGGCGGGC 
AGCACGGCCG CCAGGTCCCC CCAGTCCCCC ACCAGCAGTG ATAACAAACT CCCCGGCTTC 
CTCAGCTTCA AGTTCCTGAA ATCCCCCGAA ACCAAGAGGG ACGCGGCCAC CGAAAAGGTG 
CAGTCGCCCA CCAAGCCATT CAACCCCGGC AAGCTCTTCA ACTTTGGCAA GTCGGAAGGG 
ACCGGCGGGA ACGGGGCCAC CGCCTCCCCC CAGCCCCGTG CTGGCCCCTC TGCGGATGGG 
GGCGAGCGGC CGGGCCCCAG CAAGTCCCAC CTCAATGGGG CTCCTGAGGA CGGAGCCCGC 
GAGGAGCCGG AGCCACGAGC AGAGGAGGAG AGCGGGGGCT ACAGACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025000159.1
CDS	191..3361
Translation	MPEPPAAPHDGERPRRAPPGDERTEPAAPGGVLDKLFGKRLLQAGRYIMSHKAWMKTVPTENCDVLMTFPDTTDDHTLLWLLNHIRLGIPELIVQVRHHKHTRAYAFFVTATYESLLRGADEIGLRKPVKAEFGGGMRSFSCEEDYIYENIENELYFFTSQERQNIIRYWLENLRAKQGEALHNIHFLEGQPIIPELAARGVIQQVFPLHEQRILKRLMKSWVQAICEAQPLDEICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSILYTFTESDQTSQDICCVVFAIFNVVWATLFLEEWKRRGAEFAYKWGTLDTPAESIEEPRPQFRGVKRISPVTSAEEFYYPPWKRLLFQCLVSLPVCLACLSFVFLVMLGCFQLQELVLSVKELPRILRFLPKIVLAVIVTTCDEVYKKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLITRQFLQNVKEVSQPHLYRRLRRGELSLRNLRELSHAVLRLLAHPRAPPATGAAHEGPRGEKKCLNGGCGVPEEEEEEEERRESDSEDESVLDCGLKLKKVSFIEKAERRGTEPCSTEEESFLEEGSPTMVEKGMDPASVFELGDDEDDAEGPSGSPTKAAEPATGPRVTRRRREDEEGEEEGRRRNRASWIDPPEEDYSTQLTQAEVESCMKKYEDTFQDYQEMFIQFGYVVLFSSAFPLAAMCALVNNIIEIRSDAFKLCTGLQRPFGQRVESIGQWQKVMEAMGVLAIVVNCYLIAQCGQLQRLFPWLSPEGAIISVVVLEHFALLLKYVIQVAIPDIPAWVAEEMAKLEYQRREAFKKHERQAQHHFQQQQRRKREEEERQRHAEYQARKEREANRDEAKAEAAGQDPAHDKSQSKGKGSGGSSAHGSDKPKRPSSLLATNNVMKLKQIIPLQGKFLSGGAGAGSTAARSPQSPTSSDNKLPGFLSFKFLKSPETKRDAATEKVQSPTKPFNPGKLFNFGKSEGTGGNGATASPQPRAGPSADGGERPGPSKSHLNGAPEDGAREEPEPRAEEESGGYRL

Target ORF information:

RefSeq Version	XM_025144391.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus anoctamin 8 (ANO8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025144391.1

ORF Insert Sequence:

ATGCCCGAAC CGCCCGCGGC ACCGCACGAC GGAGAGCGGC CCCGGAGGGC CCCGCCTGGG 
GACGAACGGA CGGAGCCGGC GGCCCCGGGG GGGGTCCTGG ACAAGCTTTT CGGGAAGCGG 
CTGCTCCAAG CTGGGCGCTA CATCATGTCC CACAAAGCCT GGATGAAGAC GGTGCCCACC 
GAGAACTGTG ATGTGCTCAT GACCTTCCCA GACACCACGG ATGACCACAC GCTGCTTTGG 
CTGCTGAACC ACATCCGCCT CGGCATCCCC GAGCTCATCG TGCAGGTGCG GCACCACAAG 
CACACCCGCG CCTACGCCTT CTTCGTCACC GCCACCTACG AGAGTTTGCT GCGTGGGGCC 
GATGAGATCG GGCTGCGGAA ACCGGTGAAG GCTGAGTTTG GGGGCGGCAT GCGGAGCTTC 
TCCTGCGAGG AGGACTACAT CTATGAGAAC ATTGAGAACG AGCTTTACTT CTTCACCTCT 
CAGGAACGGC AAAACATCAT CAGGTATTGG CTGGAGAACC TGCGGGCCAA GCAGGGCGAG 
GCGCTGCACA ACATCCACTT CCTGGAGGGA CAGCCCATCA TCCCGGAGCT GGCGGCCCGC 
GGCGTCATCC AGCAGGTCTT CCCACTGCAC GAGCAGAGGA TCCTCAAGCG GCTGATGAAG 
TCGTGGGTGC AGGCGATCTG CGAGGCGCAG CCACTCGATG AGATCTGTGA CTACTTCGGG 
GTGAAGATCG CCATGTACTT CGCCTGGCTC GGCTTCTACA CCTCGGCCAT GGTGTACCCT 
GCTGTGTTCG GCTCCATCCT CTACACCTTC ACTGAGAGCG ACCAGACCAG CCAGGACATC 
TGCTGTGTGG TGTTTGCCAT CTTCAACGTC GTCTGGGCCA CGCTGTTCCT GGAGGAGTGG 
AAGCGCCGCG GGGCAGAGTT TGCCTACAAG TGGGGGACGC TGGACACCCC CGCAGAGTCC 
ATCGAGGAGC CCCGGCCCCA GTTCAGGGGG GTGAAGAGGA TCAGCCCGGT GACCAGCGCC 
GAGGAGTTCT ACTACCCGCC CTGGAAGCGG CTGCTGTTCC AGTGCCTGGT CAGCCTGCCC 
GTCTGCCTCG CCTGCCTCTC CTTCGTCTTC CTCGTCATGC TGGGCTGCTT CCAGCTCCAG 
GAGCTCGTGC TGAGCGTCAA GGAGCTGCCC CGCATCCTCC GCTTCCTGCC CAAAATCGTG 
CTGGCCGTCA TCGTCACGAC CTGCGACGAG GTTTACAAGA AGATCGCCTA CTGGCTGAAT 
GATATGGAAA ACTACCGCCT GCAGAGTGCC TACGAGAAAC ACCTCATCAT CAAAATCGTC 
CTGTTTCAGT TTGTAAATTC ATACCTAAGT CTTTTCTACA TCGGTTTCTA CCTCAAAGAC 
ATGGAGCGGC TGAAGGAGAT GCTGGCCACG CTGCTCATCA CACGGCAGTT CCTGCAGAAC 
GTCAAGGAGG TGTCGCAGCC CCACCTGTAC CGCCGCCTGC GCCGGGGGGA GCTCAGCCTG 
CGCAACCTCC GCGAGCTGTC CCACGCCGTC CTCCGCCTGC TCGCCCACCC CCGCGCACCC 
CCGGCCACTG GAGCAGCCCA CGAGGGGCCG CGGGGGGAGA AGAAGTGTCT GAACGGGGGC 
TGCGGGGTGC CCGAGGAGGA GGAGGAGGAG GAAGAGCGTC GGGAGTCAGA TTCGGAGGAT 
GAGAGCGTGC TGGACTGTGG GCTGAAGCTG AAGAAGGTGA GCTTCATCGA GAAGGCGGAG 
CGGCGCGGCA CCGAGCCCTG CAGCACCGAG GAGGAGAGCT TCCTGGAGGA GGGCAGCCCC 
ACCATGGTGG AGAAGGGAAT GGACCCCGCC TCCGTCTTCG AGCTGGGTGA CGATGAGGAT 
GATGCTGAAG GCCCCTCAGG CAGCCCAACC AAGGCAGCGG AGCCGGCCAC AGGCCCACGG 
GTCACCCGGA GGAGGAGGGA GGATGAGGAG GGTGAGGAGG AAGGGCGCAG GCGGAACCGG 
GCATCATGGA TCGATCCGCC CGAGGAGGAC TACTCGACAC AGCTGACGCA GGCGGAGGTG 
GAGAGCTGCA TGAAGAAGTA CGAGGACACC TTCCAGGACT ACCAGGAGAT GTTCATCCAG 
TTTGGCTACG TGGTGCTCTT CTCCTCTGCC TTCCCACTGG CCGCCATGTG TGCGCTGGTC 
AACAACATCA TCGAGATTCG CAGTGATGCC TTCAAACTGT GCACGGGGCT GCAGCGGCCC 
TTTGGGCAGC GAGTGGAGAG CATTGGGCAG TGGCAGAAGG TGATGGAGGC CATGGGCGTG 
TTGGCCATCG TGGTCAACTG CTACCTGATC GCGCAGTGCG GGCAGCTGCA GCGGCTCTTC 
CCTTGGCTCA GCCCCGAGGG AGCCATCATC TCGGTGGTGG TGCTGGAGCA CTTCGCCCTG 
CTGCTGAAGT ACGTCATCCA AGTGGCCATC CCTGACATCC CTGCGTGGGT GGCCGAGGAG 
ATGGCCAAGC TGGAGTACCA GCGCCGCGAG GCCTTCAAGA AGCACGAGCG CCAGGCCCAG 
CACCACTTCC AGCAGCAGCA GCGGCGCAAG CGGGAGGAGG AGGAGCGGCA GCGGCACGCC 
GAGTACCAGG CGCGCAAGGA GCGCGAGGCC AACCGCGACG AGGCCAAGGC TGAGGCTGCC 
GGGCAGGACC CGGCCCACGA CAAGAGCCAG AGCAAAGGGA AGGGCTCCGG GGGGTCCTCC 
GCGCACGGCT CCGACAAGCC CAAGCGGCCC AGCTCGCTGC TGGCCACCAA TAACGTCATG 
AAGCTGAAGC AGATCATCCC TCTGCAGGGC AAATTCCTCT CCGGGGGGGC CGGGGCGGGC 
AGCACGGCCG CCAGGTCCCC CCAGTCCCCC ACCAGCAGTG ATAACAAACT CCCCGGCTTC 
CTCAGCTTCA AGTTCCTGAA ATCCCCCGAA ACCAAGAGGG ACGCGGCCAC CGAAAAGGTG 
CAGTCGCCCA CCAAGCCATT CAACCCCGGC AAGCTCTTCA ACTTTGGCAA GTCGGAAGGG 
ACCGGCGGGA ACGGGGCCAC CGCCTCCCCC CAGCCCCGTG CTGGCCCCTC TGCGGATGGG 
GGCGAGCGGC CGGGCCCCAG CAAGTCCCAC CTCAATGGGG CTCCTGAGGA CGGAGCCCGC 
GAGGAGCCGG AGCCACGAGC AGAGGAGGAG AGCGGGGGCT ACAGACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO8 ( XM_015300106.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015155592.1 Gallus gallus anoctamin 8 (ANO8), mRNA.

Price & Availability↑

CloneID	OGa47361
Clone ID Related Accession (Same CDS sequence)	XM_015300106.1
Accession Version	XM_015300106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	anoctamin-8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456055.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAC CGCCCGCGGC ACCGCACGAC GGAGAGCGGC CCCGGAGGGC CCCGCCTGGG 
GACGAACGGA CGGAGCCGGC GGCCCCGGGG GGGGTCCTGG ACAAGCTTTT CGGGAAGCGG 
CTGCTCCAAG CTGGGCGCTA CATCATGTCC CACAAAGCCT GGATGAAGAC GGTGCCCACC 
GAGAACTGTG ATGTGCTCAT GACCTTCCCA GACACCACGG ATGACCACAC GCTGCTTTGG 
CTGCTGAACC ACATCCGCCT CGGCATCCCC GAGCTCATCG TGCAGGTGCG GCACCACAAG 
CACACCCGCG CCTACGCCTT CTTCGTCACC GCCACCTACG AGAGTTTGCT GCGTGGGGCC 
GATGAGATCG GGCTGCGGAA ACCGGTGAAG GCTGAGTTTG GGGGCGGCAT GCGGAGCTTC 
TCCTGCGAGG AGGACTACAT CTATGAGAAC ATTGAGAACG AGCTTTACTT CTTCACCTCT 
CAGGAACGGC AAAACATCAT CAGGTATTGG CTGGAGAACC TGCGGGCCAA GCAGGGCGAG 
GCGCTGCACA ACATCCACTT CCTGGAGGGA CAGCCCATCA TCCCGGAGCT GGCGGCCCGC 
GGCGTCATCC AGCAGGTCTT CCCACTGCAC GAGCAGAGGA TCCTCAAGCG GCTGATGAAG 
TCGTGGGTGC AGGCGATCTG CGAGGCGCAG CCACTCGATG AGATCTGTGA CTACTTCGGG 
GTGAAGATCG CCATGTACTT CGCCTGGCTC GGCTTCTACA CCTCGGCCAT GGTGTACCCT 
GCTGTGTTCG GCTCCATCCT CTACACCTTC ACTGAGAGCG ACCAGACCAG CCAGGACATC 
TGCTGTGTGG TGTTTGCCAT CTTCAACGTC GTCTGGGCCA CGCTGTTCCT GGAGGAGTGG 
AAGCGCCGCG GGGCAGAGTT TGCCTACAAG TGGGGGACGC TGGACACCCC CGCAGAGTCC 
ATCGAGGAGC CCCGGCCCCA GTTCAGGGGG GTGAAGAGGA TCAGCCCGGT GACCAGCGCC 
GAGGAGTTCT ACTACCCGCC CTGGAAGCGG CTGCTGTTCC AGTGCCTGGT CAGCCTGCCC 
GTCTGCCTCG CCTGCCTCTC CTTCGTCTTC CTCGTCATGC TGGGCTGCTT CCAGCTCCAG 
GAGCTCGTGC TGAGCGTCAA GGAGCTGCCC CGCATCCTCC GCTTCCTGCC CAAAATCGTG 
CTGGCCGTCA TCGTCACGAC CTGCGACGAG GTTTACAAGA AGATCGCCTA CTGGCTGAAT 
GATATGGAAA ACTACCGCCT GCAGAGTGCC TACGAGAAAC ACCTCATCAT CAAAATCGTC 
CTGTTTCAGT TTGTAAATTC ATACCTAAGT CTTTTCTACA TCGGTTTCTA CCTCAAAGAC 
ATGGAGCGGC TGAAGGAGCT CCTGCTCATC TTCTCTCTGT CCCAAAGCCT CGTGCGCCAG 
CTCAAAGAGG CGCTCCTCCC CTTCATCCTC CTCCACCTCC ACCTCTCCCT CATCTTCCTT 
AAGGGCCTCC TGGGCTTTTG CTGGAGACTG GGAGTATCCA AAATGCTGGC CACGCTGCTC 
ATCACACGGC AGTTCCTGCA GAACGTCAAG GAGGTGTCGC AGCCCCACCT GTACCGCCGC 
CTGCGCCGGG GGGAGCTCAG CCTGCGCAAC CTCCGCGAGC TGTCCCACGC CGTCCTCCGC 
CTGCTCGCCC ACCCCCGCGC ACCCCCGGCC ACTGGAGCAG CCCACGAGGG GCCGCGGGGG 
GAGAAGAAGT GTCTGAACGG GGGCTGCGGG GTGCCCGAGG AGGAGGAGGA GGAGGAAGAG 
CGTCGGGAGT CAGATTCGGA GGATGAGAGC GTGCTGGACT GTGGGCTGAA GCTGAAGAAG 
GTGAGCTTCA TCGAGAAGGC GGAGCGGCGC GGCACCGAGC CCTGCAGCAC CGAGGAGGAG 
AGCTTCCTGG AGGAGGGCAG CCCCACCATG GTGGAGAAGG GAATGGACCC CGCCTCCGTC 
TTCGAGCTGG GTGACGATGA GGATGATGCT GAAGGCCCCT CAGGCAGCCC AACCAAGGCA 
GCGGAGCCGG CCACAGGCCC ACGGGTCACC CGGAGGAGGA GGGAGGATGA GGAGGGTGAG 
GAGGAAGGGC GCAGGCGGAA CCGGGCATCA TGGATCGATC CGCCCGAGGA GGACTACTCG 
ACACAGCTGA CGCAGGCGGA GGTGGAGAGC TGCATGAAGA AGTACGAGGA CACCTTCCAG 
GACTACCAGG AGATGTTCAT CCAGTTTGGC TACGTGGTGC TCTTCTCCTC TGCCTTCCCA 
CTGGCCGCCA TGTGTGCGCT GGTCAACAAC ATCATCGAGA TTCGCAGTGA TGCCTTCAAA 
CTGTGCACGG GGCTGCAGCG GCCCTTTGGG CAGCGAGTGG AGAGCATTGG GCAGTGGCAG 
AAGGTGATGG AGGCCATGGG CGTGTTGGCC ATCGTGGTCA ACTGCTACCT GATCGCGCAG 
TGCGGGCAGC TGCAGCGGCT CTTCCCTTGG CTCAGCCCCG AGGGAGCCAT CATCTCGGTG 
GTGGTGCTGG AGCACTTCGC CCTGCTGCTG AAGTACGTCA TCCAAGTGGC CATCCCTGAC 
ATCCCTGCGT GGGTGGCCGA GGAGATGGCC AAGCTGGAGT ACCAGCGCCG CGAGGCCTTC 
AAGAAGCACG AGCGCCAGGC CCAGCACCAC TTCCAGCAGC AGCAGCGGCG CAAGCGGGAG 
GAGGAGGAGC GGCAGCGGCA CGCCGAGTAC CAGGCGCGCA AGGAGCGCGA GGCCAACCGC 
GACGAGGCCA AGGCTGAGGC TGCCGGGCAG GACCCGGCCC ACGACAAGAG CCAGAGCAAA 
GGGAAGGGCT CCGGGGGGTC CTCCGCGCAC GGCTCCGACA AGCCCAAGCG GCCCAGCTCG 
CTGCTGGCCA CCAATAACGT CATGAAGCTG AAGCAGATCA TCCCTCTGCA GGGCAAATTC 
CTCTCCGGGG GGGCCGGGGC GGGCAGCACG GCCGCCAGGT CCCCCCAGTC CCCCACCAGC 
AGTGATAACA AACTCCCCGG CTTCCTCAGC TTCAAGTTCC TGAAATCCCC CGAAACCAAG 
AGGGACGCGG CCACCGAAAA GGTGCAGTCG CCCACCAAGC CATTCAACCC CGGCAAGCTC 
TTCAACTTTG GCAAGTCGGA AGGGACCGGC GGGAACGGGG CCACCGCCTC CCCCCAGCCC 
CGTGCTGGCC CCTCTGCGGA TGGGGGCGAG CGGCCGGGCC CCAGCAAGTC CCACCTCAAT 
GGGGCTCCTG AGGACGGAGC CCGCGAGGAG CCGGAGCCAC GAGCAGAGGA GGAGAGCGGG 
GGCTACAGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015155592.1
CDS	192..3506
Translation	MPEPPAAPHDGERPRRAPPGDERTEPAAPGGVLDKLFGKRLLQAGRYIMSHKAWMKTVPTENCDVLMTFPDTTDDHTLLWLLNHIRLGIPELIVQVRHHKHTRAYAFFVTATYESLLRGADEIGLRKPVKAEFGGGMRSFSCEEDYIYENIENELYFFTSQERQNIIRYWLENLRAKQGEALHNIHFLEGQPIIPELAARGVIQQVFPLHEQRILKRLMKSWVQAICEAQPLDEICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSILYTFTESDQTSQDICCVVFAIFNVVWATLFLEEWKRRGAEFAYKWGTLDTPAESIEEPRPQFRGVKRISPVTSAEEFYYPPWKRLLFQCLVSLPVCLACLSFVFLVMLGCFQLQELVLSVKELPRILRFLPKIVLAVIVTTCDEVYKKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKELLLIFSLSQSLVRQLKEALLPFILLHLHLSLIFLKGLLGFCWRLGVSKMLATLLITRQFLQNVKEVSQPHLYRRLRRGELSLRNLRELSHAVLRLLAHPRAPPATGAAHEGPRGEKKCLNGGCGVPEEEEEEEERRESDSEDESVLDCGLKLKKVSFIEKAERRGTEPCSTEEESFLEEGSPTMVEKGMDPASVFELGDDEDDAEGPSGSPTKAAEPATGPRVTRRRREDEEGEEEGRRRNRASWIDPPEEDYSTQLTQAEVESCMKKYEDTFQDYQEMFIQFGYVVLFSSAFPLAAMCALVNNIIEIRSDAFKLCTGLQRPFGQRVESIGQWQKVMEAMGVLAIVVNCYLIAQCGQLQRLFPWLSPEGAIISVVVLEHFALLLKYVIQVAIPDIPAWVAEEMAKLEYQRREAFKKHERQAQHHFQQQQRRKREEEERQRHAEYQARKEREANRDEAKAEAAGQDPAHDKSQSKGKGSGGSSAHGSDKPKRPSSLLATNNVMKLKQIIPLQGKFLSGGAGAGSTAARSPQSPTSSDNKLPGFLSFKFLKSPETKRDAATEKVQSPTKPFNPGKLFNFGKSEGTGGNGATASPQPRAGPSADGGERPGPSKSHLNGAPEDGAREEPEPRAEEESGGYRL

Target ORF information:

RefSeq Version	XM_015300106.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus anoctamin 8 (ANO8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015300106.1

ORF Insert Sequence:

ATGCCCGAAC CGCCCGCGGC ACCGCACGAC GGAGAGCGGC CCCGGAGGGC CCCGCCTGGG 
GACGAACGGA CGGAGCCGGC GGCCCCGGGG GGGGTCCTGG ACAAGCTTTT CGGGAAGCGG 
CTGCTCCAAG CTGGGCGCTA CATCATGTCC CACAAAGCCT GGATGAAGAC GGTGCCCACC 
GAGAACTGTG ATGTGCTCAT GACCTTCCCA GACACCACGG ATGACCACAC GCTGCTTTGG 
CTGCTGAACC ACATCCGCCT CGGCATCCCC GAGCTCATCG TGCAGGTGCG GCACCACAAG 
CACACCCGCG CCTACGCCTT CTTCGTCACC GCCACCTACG AGAGTTTGCT GCGTGGGGCC 
GATGAGATCG GGCTGCGGAA ACCGGTGAAG GCTGAGTTTG GGGGCGGCAT GCGGAGCTTC 
TCCTGCGAGG AGGACTACAT CTATGAGAAC ATTGAGAACG AGCTTTACTT CTTCACCTCT 
CAGGAACGGC AAAACATCAT CAGGTATTGG CTGGAGAACC TGCGGGCCAA GCAGGGCGAG 
GCGCTGCACA ACATCCACTT CCTGGAGGGA CAGCCCATCA TCCCGGAGCT GGCGGCCCGC 
GGCGTCATCC AGCAGGTCTT CCCACTGCAC GAGCAGAGGA TCCTCAAGCG GCTGATGAAG 
TCGTGGGTGC AGGCGATCTG CGAGGCGCAG CCACTCGATG AGATCTGTGA CTACTTCGGG 
GTGAAGATCG CCATGTACTT CGCCTGGCTC GGCTTCTACA CCTCGGCCAT GGTGTACCCT 
GCTGTGTTCG GCTCCATCCT CTACACCTTC ACTGAGAGCG ACCAGACCAG CCAGGACATC 
TGCTGTGTGG TGTTTGCCAT CTTCAACGTC GTCTGGGCCA CGCTGTTCCT GGAGGAGTGG 
AAGCGCCGCG GGGCAGAGTT TGCCTACAAG TGGGGGACGC TGGACACCCC CGCAGAGTCC 
ATCGAGGAGC CCCGGCCCCA GTTCAGGGGG GTGAAGAGGA TCAGCCCGGT GACCAGCGCC 
GAGGAGTTCT ACTACCCGCC CTGGAAGCGG CTGCTGTTCC AGTGCCTGGT CAGCCTGCCC 
GTCTGCCTCG CCTGCCTCTC CTTCGTCTTC CTCGTCATGC TGGGCTGCTT CCAGCTCCAG 
GAGCTCGTGC TGAGCGTCAA GGAGCTGCCC CGCATCCTCC GCTTCCTGCC CAAAATCGTG 
CTGGCCGTCA TCGTCACGAC CTGCGACGAG GTTTACAAGA AGATCGCCTA CTGGCTGAAT 
GATATGGAAA ACTACCGCCT GCAGAGTGCC TACGAGAAAC ACCTCATCAT CAAAATCGTC 
CTGTTTCAGT TTGTAAATTC ATACCTAAGT CTTTTCTACA TCGGTTTCTA CCTCAAAGAC 
ATGGAGCGGC TGAAGGAGCT CCTGCTCATC TTCTCTCTGT CCCAAAGCCT CGTGCGCCAG 
CTCAAAGAGG CGCTCCTCCC CTTCATCCTC CTCCACCTCC ACCTCTCCCT CATCTTCCTT 
AAGGGCCTCC TGGGCTTTTG CTGGAGACTG GGAGTATCCA AAATGCTGGC CACGCTGCTC 
ATCACACGGC AGTTCCTGCA GAACGTCAAG GAGGTGTCGC AGCCCCACCT GTACCGCCGC 
CTGCGCCGGG GGGAGCTCAG CCTGCGCAAC CTCCGCGAGC TGTCCCACGC CGTCCTCCGC 
CTGCTCGCCC ACCCCCGCGC ACCCCCGGCC ACTGGAGCAG CCCACGAGGG GCCGCGGGGG 
GAGAAGAAGT GTCTGAACGG GGGCTGCGGG GTGCCCGAGG AGGAGGAGGA GGAGGAAGAG 
CGTCGGGAGT CAGATTCGGA GGATGAGAGC GTGCTGGACT GTGGGCTGAA GCTGAAGAAG 
GTGAGCTTCA TCGAGAAGGC GGAGCGGCGC GGCACCGAGC CCTGCAGCAC CGAGGAGGAG 
AGCTTCCTGG AGGAGGGCAG CCCCACCATG GTGGAGAAGG GAATGGACCC CGCCTCCGTC 
TTCGAGCTGG GTGACGATGA GGATGATGCT GAAGGCCCCT CAGGCAGCCC AACCAAGGCA 
GCGGAGCCGG CCACAGGCCC ACGGGTCACC CGGAGGAGGA GGGAGGATGA GGAGGGTGAG 
GAGGAAGGGC GCAGGCGGAA CCGGGCATCA TGGATCGATC CGCCCGAGGA GGACTACTCG 
ACACAGCTGA CGCAGGCGGA GGTGGAGAGC TGCATGAAGA AGTACGAGGA CACCTTCCAG 
GACTACCAGG AGATGTTCAT CCAGTTTGGC TACGTGGTGC TCTTCTCCTC TGCCTTCCCA 
CTGGCCGCCA TGTGTGCGCT GGTCAACAAC ATCATCGAGA TTCGCAGTGA TGCCTTCAAA 
CTGTGCACGG GGCTGCAGCG GCCCTTTGGG CAGCGAGTGG AGAGCATTGG GCAGTGGCAG 
AAGGTGATGG AGGCCATGGG CGTGTTGGCC ATCGTGGTCA ACTGCTACCT GATCGCGCAG 
TGCGGGCAGC TGCAGCGGCT CTTCCCTTGG CTCAGCCCCG AGGGAGCCAT CATCTCGGTG 
GTGGTGCTGG AGCACTTCGC CCTGCTGCTG AAGTACGTCA TCCAAGTGGC CATCCCTGAC 
ATCCCTGCGT GGGTGGCCGA GGAGATGGCC AAGCTGGAGT ACCAGCGCCG CGAGGCCTTC 
AAGAAGCACG AGCGCCAGGC CCAGCACCAC TTCCAGCAGC AGCAGCGGCG CAAGCGGGAG 
GAGGAGGAGC GGCAGCGGCA CGCCGAGTAC CAGGCGCGCA AGGAGCGCGA GGCCAACCGC 
GACGAGGCCA AGGCTGAGGC TGCCGGGCAG GACCCGGCCC ACGACAAGAG CCAGAGCAAA 
GGGAAGGGCT CCGGGGGGTC CTCCGCGCAC GGCTCCGACA AGCCCAAGCG GCCCAGCTCG 
CTGCTGGCCA CCAATAACGT CATGAAGCTG AAGCAGATCA TCCCTCTGCA GGGCAAATTC 
CTCTCCGGGG GGGCCGGGGC GGGCAGCACG GCCGCCAGGT CCCCCCAGTC CCCCACCAGC 
AGTGATAACA AACTCCCCGG CTTCCTCAGC TTCAAGTTCC TGAAATCCCC CGAAACCAAG 
AGGGACGCGG CCACCGAAAA GGTGCAGTCG CCCACCAAGC CATTCAACCC CGGCAAGCTC 
TTCAACTTTG GCAAGTCGGA AGGGACCGGC GGGAACGGGG CCACCGCCTC CCCCCAGCCC 
CGTGCTGGCC CCTCTGCGGA TGGGGGCGAG CGGCCGGGCC CCAGCAAGTC CCACCTCAAT 
GGGGCTCCTG AGGACGGAGC CCGCGAGGAG CCGGAGCCAC GAGCAGAGGA GGAGAGCGGG 
GGCTACAGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ANO8 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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