Dpse\ade3 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\ade3
Entrez Gene ID	4817813
Full Name	GA25439 gene product from transcript GA25439-RA
Synonyms	Dpse\GA16345,Dpse\GA25439,Dpse_GA16345,Dpse_GA16555,GA16345,GA16555,GA25439,GART,Gart,Gart/ade3,ade3,dpse_GLEANR_17006,gart
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001357356.3	XP_001357392.2	ade3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\ade3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\ade3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe11017	XM_001357356.3 Latest version!	Drosophila pseudoobscura pseudoobscura ade3 (Dpse\ade3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\ade3 ( XM_001357356.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001357392.2 Drosophila pseudoobscura pseudoobscura ade3 (Dpse\ade3), mRNA.

Price & Availability↑

CloneID	ODe11017
Clone ID Related Accession (Same CDS sequence)	XM_001357356.3
Accession Version	XM_001357356.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	ade3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589966). On Dec 22, 2015 this sequence version replaced XM_001357356.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCATA GCGTCTTGGT CATCGGCAGC GGCGGGCGGG AGCATGCCAT CTGCTGGAAA 
TTGTCGCAGT CGACGCTGGT GAAGCAGATC TATGCCCTGC CTGGCAGCTT TGGCATCCAA 
CAGGTGGAAA AGTGCCGCAA TTTGGATGCA AAAGTCTTGG ATCCAAAGGA TTTTGAAGCC 
ATTGCCAAAT GGAGCAAGAA AAATGAAATC TCTCTAGTGG TCGTTGGACC CGAAGATCCC 
TTGGCCTTGG GTCTGGGCGA TGTGCTGCAA AAGGAGGGCA TCCCATGCTT TGGACCCGGC 
AAGCAGGGGG CCCAAATCGA GGCGGACAAG AAGTGGGCCA AGGACTTTAT GCTGCGCCAT 
GGAATACCAA CGGCTCGATA TGAGAGCTTC ACGGATACGA ACAAGGCCAA GGCGTTCATC 
AGGAGTGCAC CCTATCAGGC TCTGGTGGTG AAGGCCGCTG GTTTGGCTGC AGGCAAGGGT 
GTTGTGGTGG CCGCCAATGT GGATGAAGCC TGCCAAGCGG TGGATGAGAT ATTGGGAGAC 
CTGAAATACG GACAAGCTGG GGCCACCCTT GTCGTAGAGG AGCTTCTCGA GGGCGAGGAA 
ATATCGGTTC TAGCCTTCAC CGATGGCAAG AGTGTTAGGG CCATGTTGCC TGCCCAGGAT 
CACAAGCGTT TGGGCAATGG AGATACAGGA CCAAACACCG GAGGAATGGG CGCCTACTGT 
CCCTGTCCTT TGATCAGTCA GCCCGCGTTG GAGCTGGTCC AGCGAGCCGT GCTGGAGAGA 
GCTGTTCAGG GTCTGATCAA GGAGCGCATC ACCTATCAGG GTGTTCTCTA TGCGGGACTC 
ATGCTGACAC GCGATGGACC CCGCGTCCTC GAGTTCAACT GTCGCTTTGG CGATCCCGAA 
ACGCAGGTCA TTCTGCCGCT CCTCGAAACT GATCTTTTCG AGGTGATGCA GGCCTGCTGC 
AGCGGTCAGC TGGATAGGCT GCCCCTCCAG TGGCGCAGCG GCGTGAGTGC TGTGGGTGTG 
GTCCTCGCAA GTGCTGGCTA TCCAGAGACC TCCACGAAGG GTTGCCTCAT CACTGGACTA 
CCTGATGTCA ATTCGCCCAC TCAATTGATT TTCCACAGCG GTCTTTCTGT AAACAAACAA 
AAGGAGGCCC TCACGAATGG CGGACGTGTG CTGATAGCAA TTGCCTTGGA TGCCAGCCTC 
AAGGAGGCGG CTGCAAAGGC CACAAAACTT GCAGGAACTA TCACTTTTGC CGGCACAGGA 
GCTCAGTATC GCACGGACAT TGCCCAGAAA GCATTTAAAA TAGCCATTGC CACGGCGCCA 
GGCCTCAGCT ACAAAGACAG CGGCGTGGAC ATTGATGCCG GCGATGCTTT GGTCCAACGC 
ATCAAGCCCT TGTCGCGTGG CACTCAAAGA CCTGGGGTCC TTGGCGGTTT GGGTGGCTTT 
GGTGGTCTGT TTCGGCTGAA GGATCTCAGC TACAAGGAGC CCGTAATTGC AGAGGCCACG 
CAGGGTGTGG GCGCCAAGAT CCAGTTGGCC CTGCAGAATG AATTGTATGA GAATATTGGC 
TACGATCTGT TTGCCATGTC TGCCAATGAT CTGCTGGAAC TGGGCGCCGA GCCGGTAGCT 
TTTCTGGATT ACATAGCCTG TGGCAAGCTG CATGTGCCAC TGGCCGCTCA GCTCGTGAAG 
GGAATGGCTG ATGGCTGTCG GGATGCCAAG TGCGCATTAG TAGGTGGCGA AACAGCGGAG 
ATGCCCTCGC TGTACGCCCC CGGGCAGCAC GACATGGCCG GCTACTGTGT GGGCATCGTA 
GAGCAGGCTC GTGTCCTGCC ACGCTTTGAT CTGTACGAAC CGGAGGATCT GCTCGTGGGC 
CTGCCGTCCT CGGGCCTCCA CTGTGCTGGC TTCAACGAGA TCCTCACCCA GTTGGCCGCT 
TCGAAAGTGA ATCTCAAGGA GTGCTCTCCT GTCGGCGGGG GCAAGCATGG CCTTAGCCTG 
GCCCAGGTCC TGGGTACGCC CACACGCCTG TACGTGCAAC AGCTGCTGCC CCATCTGCAG 
GCTGGCAACC AGATCAAAGC TGTGGCCCAT GTCACGCATG GCCTGCTGCA CGATGTCCAG 
CGCTTGCTGC CCGAAGGATT CGAGGTCACC CTGGACTTTG GGGCAGTGCC CGTGCCGGAT 
GTCTTTGGCT GGCTGGCGGG ACAGCTGCAG CTGAGTGCCC AGACCCTGCT GGAGCGACAC 
AACTGCGGCA TTGGCATGGT GCTGGTTCTC CCACAGAGCA GTCTTCTGTG GCGCACAGCC 
CTGCCGGGGG CCAAGGTTCT GGGCGTGTTG AATCGTCAAG CGAAAGCCTC CGGCGGTGCT 
CCGCGCGTGA AAGTGCGAAA TTTTGTGGAG CAACTGCAGA AGTTGGCTGC ACCCTTTGGG 
GGTTTGGGGG AAACGCAGCT GCCCGAGGAA GTCAAGGATG TGCCCTCTTC GGGAGTGAAG 
GCAACGACAC GTGAAGAATG CTTTGAGAAT GCCGTGGGAC GTCGCCTGAC TCGCGTGCCC 
AATCATTACG TGGATCCCAT ACTCATCTTG GGTACCGATG GTGTGGGCAC CAAGCTGAAG 
ATTGCCCAGC AGACCCATCG GAATGCCAGC GTGGGCATCG ATCTGGTGGC CATGTGCGTC 
AACGATATAC TCTGCAATGG CGCCGAACCA TTTAGCTTTT CCAGCTATTA TGCCTGCGGC 
AAATGGCAGG CCGCCTTGGC TGCGGAAGTC AACGCTGGTG TACAGGAAGG CGCCAGCCAG 
GCCAATAGTA GTTTTGTGGC CTCCCATAGT GCTGCCCTAC CACTCCTGTA TGAGCCGCAG 
GTCTATGATC TGGCTGGCTT TGCTTTGGGC ATAGCCGAAC GCTCGGGCAT CCTGCCACGC 
CTGGATGAGA TTCAGCCCGG AGATGTGCTC ATTGGCTTGC CCTCATCGGG TGTCCACAGC 
AACGGTTTCA GCCTCGTTCA TGCGGTGCTC AAGCGTGCCG GCTTGGGCCT CAACGATCGG 
GCGCCATTCA GCGAGAAAAC ACTGGGGGAG GAGCTCCTAG TGCCCACCAA GATCTATGTG 
AAGGCATTGT CAGCGCTGCT CTCTCGACCC AATCATGGGA TCAAGGCGCT GGCGCACATC 
ACTGGCGGTG GCCTGAGCGA GAATATACCC CGAGTGCTGC GCAAGGAATT GGCCGTTCGA 
TTGGACGCCA ACAAATACCC GCTGCCCCCA GTATTTGCCT GGCTGGCAGC AGCAGGAAAC 
ATCAGCTCCA CAGAGCTGCA GAGGACCTAC AACTGTGGCT TGGGTCTGGT GTTGGTGGTG 
GGAGCCACGG AAGTGGATGG CGTGCTCCGG GAGCTCCGCT ATCCACAGCG CGCCTCTGTT 
GTGGGCGAAG TAGTGGCCCG AAAGGATCCG AAGAAACCCC AGGTGGTGGT CCAGAACTTT 
GAGGCATCGC TGGCCAGGAC CCAGAGAATG CTATCACAAC CACGGAAACG CGTTGCAGTG 
CTCATTTCAG GAAAAGGCAG CAATCTGCAG GCCCTCATCG ATGCCATCCG TGACTCGGCT 
CAAGGCGTGT ACGCAGAGAT TGTCCTCGTC ATCAGCAACA AGGCGGGTGT CTTGGGTCTG 
GAGAGGGCCG CCAAGGCGGG CATACCCTCG ATGGTCATTT CGCACAAGGA CTTCCCCAGT 
CGCGAGGTCT ACGATGTTGA ACTAACGCGC CATCTCAAGA CAGCTCGCGT GGAATTCATC 
TGCCTGGCGG GCTTCATGCG CATCCTCAGT GTTCCCTTTG TACGCGAATG GCGTGGCCGT 
CTCATCAACA TTCATCCCTC GCTGCTGCCT AAATTTCCGG GGCTACATGT CCAAAAGCAG 
GCCCTGGAGG CGGGTGAAAC AGAGTCCGGG TGCACGGTTC ACTATGTGGA CGAGGGCGTC 
GACACGGGAG CCATAATCGT TCAGGCCGCT GTTCCCATAC TGCCCGGGGA TGATGAGGAG 
ACGTTGACCC AGCGCATCCA TTATGCCGAA CATTGGGCTT TTCCGCGTGC ACTGGCGCTG 
TTGGCCAGCG GTGCCCTGCG TCGGGTGTCA GAGGTAAAGA AGGAGGCGCC AAAGGATATC 
AAGGATAGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001357392.2
CDS	535..4629
Translation	MSHSVLVIGSGGREHAICWKLSQSTLVKQIYALPGSFGIQQVEKCRNLDAKVLDPKDFEAIAKWSKKNEISLVVVGPEDPLALGLGDVLQKEGIPCFGPGKQGAQIEADKKWAKDFMLRHGIPTARYESFTDTNKAKAFIRSAPYQALVVKAAGLAAGKGVVVAANVDEACQAVDEILGDLKYGQAGATLVVEELLEGEEISVLAFTDGKSVRAMLPAQDHKRLGNGDTGPNTGGMGAYCPCPLISQPALELVQRAVLERAVQGLIKERITYQGVLYAGLMLTRDGPRVLEFNCRFGDPETQVILPLLETDLFEVMQACCSGQLDRLPLQWRSGVSAVGVVLASAGYPETSTKGCLITGLPDVNSPTQLIFHSGLSVNKQKEALTNGGRVLIAIALDASLKEAAAKATKLAGTITFAGTGAQYRTDIAQKAFKIAIATAPGLSYKDSGVDIDAGDALVQRIKPLSRGTQRPGVLGGLGGFGGLFRLKDLSYKEPVIAEATQGVGAKIQLALQNELYENIGYDLFAMSANDLLELGAEPVAFLDYIACGKLHVPLAAQLVKGMADGCRDAKCALVGGETAEMPSLYAPGQHDMAGYCVGIVEQARVLPRFDLYEPEDLLVGLPSSGLHCAGFNEILTQLAASKVNLKECSPVGGGKHGLSLAQVLGTPTRLYVQQLLPHLQAGNQIKAVAHVTHGLLHDVQRLLPEGFEVTLDFGAVPVPDVFGWLAGQLQLSAQTLLERHNCGIGMVLVLPQSSLLWRTALPGAKVLGVLNRQAKASGGAPRVKVRNFVEQLQKLAAPFGGLGETQLPEEVKDVPSSGVKATTREECFENAVGRRLTRVPNHYVDPILILGTDGVGTKLKIAQQTHRNASVGIDLVAMCVNDILCNGAEPFSFSSYYACGKWQAALAAEVNAGVQEGASQANSSFVASHSAALPLLYEPQVYDLAGFALGIAERSGILPRLDEIQPGDVLIGLPSSGVHSNGFSLVHAVLKRAGLGLNDRAPFSEKTLGEELLVPTKIYVKALSALLSRPNHGIKALAHITGGGLSENIPRVLRKELAVRLDANKYPLPPVFAWLAAAGNISSTELQRTYNCGLGLVLVVGATEVDGVLRELRYPQRASVVGEVVARKDPKKPQVVVQNFEASLARTQRMLSQPRKRVAVLISGKGSNLQALIDAIRDSAQGVYAEIVLVISNKAGVLGLERAAKAGIPSMVISHKDFPSREVYDVELTRHLKTARVEFICLAGFMRILSVPFVREWRGRLINIHPSLLPKFPGLHVQKQALEAGETESGCTVHYVDEGVDTGAIIVQAAVPILPGDDEETLTQRIHYAEHWAFPRALALLASGALRRVSEVKKEAPKDIKDSQ

Target ORF information:

RefSeq Version	XM_001357356.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura ade3 (Dpse\ade3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001357356.3

ORF Insert Sequence:

ATGTCGCATA GCGTCTTGGT CATCGGCAGC GGCGGGCGGG AGCATGCCAT CTGCTGGAAA 
TTGTCGCAGT CGACGCTGGT GAAGCAGATC TATGCCCTGC CTGGCAGCTT TGGCATCCAA 
CAGGTGGAAA AGTGCCGCAA TTTGGATGCA AAAGTCTTGG ATCCAAAGGA TTTTGAAGCC 
ATTGCCAAAT GGAGCAAGAA AAATGAAATC TCTCTAGTGG TCGTTGGACC CGAAGATCCC 
TTGGCCTTGG GTCTGGGCGA TGTGCTGCAA AAGGAGGGCA TCCCATGCTT TGGACCCGGC 
AAGCAGGGGG CCCAAATCGA GGCGGACAAG AAGTGGGCCA AGGACTTTAT GCTGCGCCAT 
GGAATACCAA CGGCTCGATA TGAGAGCTTC ACGGATACGA ACAAGGCCAA GGCGTTCATC 
AGGAGTGCAC CCTATCAGGC TCTGGTGGTG AAGGCCGCTG GTTTGGCTGC AGGCAAGGGT 
GTTGTGGTGG CCGCCAATGT GGATGAAGCC TGCCAAGCGG TGGATGAGAT ATTGGGAGAC 
CTGAAATACG GACAAGCTGG GGCCACCCTT GTCGTAGAGG AGCTTCTCGA GGGCGAGGAA 
ATATCGGTTC TAGCCTTCAC CGATGGCAAG AGTGTTAGGG CCATGTTGCC TGCCCAGGAT 
CACAAGCGTT TGGGCAATGG AGATACAGGA CCAAACACCG GAGGAATGGG CGCCTACTGT 
CCCTGTCCTT TGATCAGTCA GCCCGCGTTG GAGCTGGTCC AGCGAGCCGT GCTGGAGAGA 
GCTGTTCAGG GTCTGATCAA GGAGCGCATC ACCTATCAGG GTGTTCTCTA TGCGGGACTC 
ATGCTGACAC GCGATGGACC CCGCGTCCTC GAGTTCAACT GTCGCTTTGG CGATCCCGAA 
ACGCAGGTCA TTCTGCCGCT CCTCGAAACT GATCTTTTCG AGGTGATGCA GGCCTGCTGC 
AGCGGTCAGC TGGATAGGCT GCCCCTCCAG TGGCGCAGCG GCGTGAGTGC TGTGGGTGTG 
GTCCTCGCAA GTGCTGGCTA TCCAGAGACC TCCACGAAGG GTTGCCTCAT CACTGGACTA 
CCTGATGTCA ATTCGCCCAC TCAATTGATT TTCCACAGCG GTCTTTCTGT AAACAAACAA 
AAGGAGGCCC TCACGAATGG CGGACGTGTG CTGATAGCAA TTGCCTTGGA TGCCAGCCTC 
AAGGAGGCGG CTGCAAAGGC CACAAAACTT GCAGGAACTA TCACTTTTGC CGGCACAGGA 
GCTCAGTATC GCACGGACAT TGCCCAGAAA GCATTTAAAA TAGCCATTGC CACGGCGCCA 
GGCCTCAGCT ACAAAGACAG CGGCGTGGAC ATTGATGCCG GCGATGCTTT GGTCCAACGC 
ATCAAGCCCT TGTCGCGTGG CACTCAAAGA CCTGGGGTCC TTGGCGGTTT GGGTGGCTTT 
GGTGGTCTGT TTCGGCTGAA GGATCTCAGC TACAAGGAGC CCGTAATTGC AGAGGCCACG 
CAGGGTGTGG GCGCCAAGAT CCAGTTGGCC CTGCAGAATG AATTGTATGA GAATATTGGC 
TACGATCTGT TTGCCATGTC TGCCAATGAT CTGCTGGAAC TGGGCGCCGA GCCGGTAGCT 
TTTCTGGATT ACATAGCCTG TGGCAAGCTG CATGTGCCAC TGGCCGCTCA GCTCGTGAAG 
GGAATGGCTG ATGGCTGTCG GGATGCCAAG TGCGCATTAG TAGGTGGCGA AACAGCGGAG 
ATGCCCTCGC TGTACGCCCC CGGGCAGCAC GACATGGCCG GCTACTGTGT GGGCATCGTA 
GAGCAGGCTC GTGTCCTGCC ACGCTTTGAT CTGTACGAAC CGGAGGATCT GCTCGTGGGC 
CTGCCGTCCT CGGGCCTCCA CTGTGCTGGC TTCAACGAGA TCCTCACCCA GTTGGCCGCT 
TCGAAAGTGA ATCTCAAGGA GTGCTCTCCT GTCGGCGGGG GCAAGCATGG CCTTAGCCTG 
GCCCAGGTCC TGGGTACGCC CACACGCCTG TACGTGCAAC AGCTGCTGCC CCATCTGCAG 
GCTGGCAACC AGATCAAAGC TGTGGCCCAT GTCACGCATG GCCTGCTGCA CGATGTCCAG 
CGCTTGCTGC CCGAAGGATT CGAGGTCACC CTGGACTTTG GGGCAGTGCC CGTGCCGGAT 
GTCTTTGGCT GGCTGGCGGG ACAGCTGCAG CTGAGTGCCC AGACCCTGCT GGAGCGACAC 
AACTGCGGCA TTGGCATGGT GCTGGTTCTC CCACAGAGCA GTCTTCTGTG GCGCACAGCC 
CTGCCGGGGG CCAAGGTTCT GGGCGTGTTG AATCGTCAAG CGAAAGCCTC CGGCGGTGCT 
CCGCGCGTGA AAGTGCGAAA TTTTGTGGAG CAACTGCAGA AGTTGGCTGC ACCCTTTGGG 
GGTTTGGGGG AAACGCAGCT GCCCGAGGAA GTCAAGGATG TGCCCTCTTC GGGAGTGAAG 
GCAACGACAC GTGAAGAATG CTTTGAGAAT GCCGTGGGAC GTCGCCTGAC TCGCGTGCCC 
AATCATTACG TGGATCCCAT ACTCATCTTG GGTACCGATG GTGTGGGCAC CAAGCTGAAG 
ATTGCCCAGC AGACCCATCG GAATGCCAGC GTGGGCATCG ATCTGGTGGC CATGTGCGTC 
AACGATATAC TCTGCAATGG CGCCGAACCA TTTAGCTTTT CCAGCTATTA TGCCTGCGGC 
AAATGGCAGG CCGCCTTGGC TGCGGAAGTC AACGCTGGTG TACAGGAAGG CGCCAGCCAG 
GCCAATAGTA GTTTTGTGGC CTCCCATAGT GCTGCCCTAC CACTCCTGTA TGAGCCGCAG 
GTCTATGATC TGGCTGGCTT TGCTTTGGGC ATAGCCGAAC GCTCGGGCAT CCTGCCACGC 
CTGGATGAGA TTCAGCCCGG AGATGTGCTC ATTGGCTTGC CCTCATCGGG TGTCCACAGC 
AACGGTTTCA GCCTCGTTCA TGCGGTGCTC AAGCGTGCCG GCTTGGGCCT CAACGATCGG 
GCGCCATTCA GCGAGAAAAC ACTGGGGGAG GAGCTCCTAG TGCCCACCAA GATCTATGTG 
AAGGCATTGT CAGCGCTGCT CTCTCGACCC AATCATGGGA TCAAGGCGCT GGCGCACATC 
ACTGGCGGTG GCCTGAGCGA GAATATACCC CGAGTGCTGC GCAAGGAATT GGCCGTTCGA 
TTGGACGCCA ACAAATACCC GCTGCCCCCA GTATTTGCCT GGCTGGCAGC AGCAGGAAAC 
ATCAGCTCCA CAGAGCTGCA GAGGACCTAC AACTGTGGCT TGGGTCTGGT GTTGGTGGTG 
GGAGCCACGG AAGTGGATGG CGTGCTCCGG GAGCTCCGCT ATCCACAGCG CGCCTCTGTT 
GTGGGCGAAG TAGTGGCCCG AAAGGATCCG AAGAAACCCC AGGTGGTGGT CCAGAACTTT 
GAGGCATCGC TGGCCAGGAC CCAGAGAATG CTATCACAAC CACGGAAACG CGTTGCAGTG 
CTCATTTCAG GAAAAGGCAG CAATCTGCAG GCCCTCATCG ATGCCATCCG TGACTCGGCT 
CAAGGCGTGT ACGCAGAGAT TGTCCTCGTC ATCAGCAACA AGGCGGGTGT CTTGGGTCTG 
GAGAGGGCCG CCAAGGCGGG CATACCCTCG ATGGTCATTT CGCACAAGGA CTTCCCCAGT 
CGCGAGGTCT ACGATGTTGA ACTAACGCGC CATCTCAAGA CAGCTCGCGT GGAATTCATC 
TGCCTGGCGG GCTTCATGCG CATCCTCAGT GTTCCCTTTG TACGCGAATG GCGTGGCCGT 
CTCATCAACA TTCATCCCTC GCTGCTGCCT AAATTTCCGG GGCTACATGT CCAAAAGCAG 
GCCCTGGAGG CGGGTGAAAC AGAGTCCGGG TGCACGGTTC ACTATGTGGA CGAGGGCGTC 
GACACGGGAG CCATAATCGT TCAGGCCGCT GTTCCCATAC TGCCCGGGGA TGATGAGGAG 
ACGTTGACCC AGCGCATCCA TTATGCCGAA CATTGGGCTT TTCCGCGTGC ACTGGCGCTG 
TTGGCCAGCG GTGCCCTGCG TCGGGTGTCA GAGGTAAAGA AGGAGGCGCC AAAGGATATC 
AAGGATAGCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\ade3 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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