Dpse\GA16382 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA16382
Entrez Gene ID	4817125
Full Name	GA16382 gene product from transcript GA16382-RA
Synonyms	GA16382,dpse_GLEANR_2469
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001356611.3	XP_001356647.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA16382 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA16382 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe15625	XM_001356611.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA16382), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA16382 ( XM_001356611.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001356647.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA16382), mRNA.

Price & Availability↑

CloneID	ODe15625
Clone ID Related Accession (Same CDS sequence)	XM_001356611.3
Accession Version	XM_001356611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589964). On Dec 22, 2015 this sequence version replaced XM_001356611.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGCGCT TGAAGAAATT GTCGAAGAGT CACAGCGTGA CGGTTACACC GGAGAGCGAT 
GGCAGCAGTG CGACGACGGC AATGGAGACG GCGACGGTAG CGGCTGCTTC GACGGCGTCC 
GACACAAATG GAGGGTCCGG CGCAGTCAGT GTCCTGAATC CAGATATATT TAAGCATTTC 
GACAAGGCCG GCAGAAGTGA ATTTTTGAAA TACCTCAAAC AACAGCTGCA AGAAAGTGCC 
GACAGTCCCG TATACGATCA GAATGGAGTC TTCAGGCAGG GCGTCTCGAA TGCCATATAC 
GAGCTGCTCT GGCAAGCATT GCGCTTCACC CACAAGAAGG ATCTTATCCT TCATCTGCTC 
ACAGAAGTTG TGGCCATACA TAAGGATTTC CCTAGTCTCA TTGTGGATGT GGTCAATATT 
TTGGACAGCG AGACTTCCCT TATGACAGAG GGGCTGCAAG AGGAGCGTCA TGGTTTTGTG 
CAGCTCGTCA AGGATATGGA CAAGGTGGTG CCAGAGAGTT TGCTCAAGGA GCGCCTGGAG 
ATTGATACGC TGCAGGAGGT GGGGATTGTG AAGAACAAGG GTTTCTACTC CAAGTTCATC 
AAGGTCAAAA CCAAGTTGTA CTACAAGCAG CGACGCTTTA ATCTATTCCG CGAGGAAAGC 
GAAGGCTTTG CCAAGCTGAT AACCGAGCTG AATCAGGACT TTGAGGAGAG CACCACTCCC 
GAGAGCATTA TGGACATTAT AAAATCGCTG ATAGGTTGCT TCAATCTGGA TCCCAATCGC 
GTGCTGGATA TCATAATTGA ATCGTTTGAA ACCCGCCCGG ATCGTTGGCA ATTGTTTATA 
CCCTTGCTAC GCAGCTATAT GCCCACGGGT GCCATTATTT GTGAAGTACT CGGCTACAAG 
TTCTGCCATT TTAAGGACTC TCGTACGCCC CGATCTCTGT ACCATGTGTG CGCGCTGCTG 
TTGAAGCACG AGGTGATTGA ACTGAACGAT GTGTACGTCT GGTTGACCCC CAATGATGGC 
AGCATCAAGG CCAACTGGGA GCACGAGCTG TCGGATGCCC GAGAAATGGT GCGAAAACTG 
AATCTGATCT CCACCAACAA AAAGGAAGAC GACAAAGAGC CACCACCACC GCCGCCAACC 
GTTAAGCGGT TTGATGAGGA AAAGTACAAT CTAAATCAAA AGTTTGGTCT CTGCGAGGCT 
CTGCTCAAGG TGGGGGACTG GGAGAATGCC CACAGAATCA TACAAAAACT GCCGGAACAA 
TCGGTGGTCA TACAAGAGCC CATTGCCCGT GCCATTGTCG AACTGATTCA TTTGTCCGTG 
GAGCATGTCT ACTACAAGAA ATGCTTCAAA GCCCCCGCCG GACGCAAACA GAGTCGCAAT 
CGTTTGTACG ATGATGCCAA GCTGGTGGCC AAGATGCAGG CCCGTGAATT TGTTGATCTG 
CGGAAGTATA CGTGGCCGAT GGCTAATGTT TTGGGCCCGG CGATGCACTG TGACACGGTG 
CTAATGTACA AACTGATACG GATTATGAGC AAGCTAATTG TGGAGATGGG TGTCGACAGC 
ATGAATGGCC CGCCGCCTAA TTCAGAGGCG GAGAAGCATT ACTACGACAT AATGACCTGC 
ATGGATTCGT GCATACTGCC ATCGCTGCTG TATTTGGACA ATAATTGTTC CATGTCCGAG 
GAGATTTGGG CTGTGCTCAA GTATTTCCCG TATCATTTTC GATACAGTTT GTATGCCAGA 
TGGAAAAACG ATAGCTACCA GCTGCATCCG AATCTGATAC GACGCAGTGG CTTGGCCATG 
CGCGACATCA AGGGACTGAT GAAGCGTGTC AGCAAGGAAA ATGTAAAGCA ATTGGGTCGA 
CTGGTGGGCA AGTACTGTCA CTGTGCCCCT GGACTGATGT TTGATTATAT TCTCCTGCAA 
ATCCAGATGT ACGACAACCT TATCGCACCT GTCTGTGACA TGTTGAAGTT CCTGACATCT 
CTGTCCTTTG ATTGCCTGGG CTATTGCATC ATTGAATCGC TGACTCTGAC GGGTCGTGGC 
AGGTTCAAAG ACGACGGCAC TTCGCTTTCC CTGTGGCTGC AAAGTCTGGC CTCCTTTTGT 
GGGACCATCT ACAAAAAATA CAGCATCGAA TTGAGCGGCC TGCTGCAGTA TGTGGCCAAT 
CAATTGAAGT CACAGAAGAG CCTGGATCTG CTAATACTAC GCGAGATTGT GCACAAGATG 
GCTGGCGTGG AGTCGTGCGA GGAGATGACC AACGAACAGT TGCAGGCCTT GTGCGGGGGC 
GAACAGCTGC GCGGCGAGGC GGGATATTTC AGTCAAGTGA GGAACACTAA GAAGTCATCA 
AATCGGCTGA AGGAGGCCCT GGCCAACAAT GATCTGGCTG TGGCCATTTG TCTGCTGATG 
GCCCAGCAGA AGCATTGTGT GATCTATCGT GAAACGGCGG CGCATAGTCA TCTGAAGCTG 
GTGGGGAATC TTTATGATCA GTGCCAGGAC ACGCTAGTGC AATTTGGCAC CTTTTTGGGT 
TCCACCTATT CGGTGGATGA GTATGTGGAG CGACTGCCAT CGATCATAAC GATGCTAAGG 
GAGTATCACA TCAATACGGA TGTGGCATTC TTTCTGGCGA GGCCCATGTT TACACATCAG 
ATTAATCAAA AGTACGATCA ATTGCGTAAA TCCGATCCGA ATGCCAAAAA ACTGACCACC 
ACACAGAAAA TGCAAAAGTA TCTGGACGCC ACACAACTCA TCATGAACCC CATTGTGGAG 
TCTGTGCGTC CGCTGCATAG TGCCAAAGTG TGGGAGGACA TAAGTCCGCA GTTTTTGGTG 
ACCTTCTGGA GCCTCAGCAT GTACGATCTG CATGTCCCCA ACGATAGCTA CCAGCGTGAG 
ATTGGAAAAC TCAAGCAATT GGCCCATCAG GCGGCCGAGG GCAAGGACTC CAACCAGTCC 
AAGAATAAAA AAGAACAAGA GCGATACATT GCTTTGATGG AGAAGCTAAA TGATGAGCGG 
AAGAAGCAAC ACGAGCATGT GGACAAGATA CAGCAGCGTC TGCAAGAGCA GAGGGACAGC 
TGGTTCCTGC TGCGTTCGGC CAAGTCCGCC AAGAACGATA CCATTACGCA GTTCCTGCAA 
TTGTGCCTGT TTCCGCGCTG CACTTTCACT GCCCTGGATG CGCTCTATTG CGCAAAGTTT 
GTCCATACCA TCCACAATCT AAAGACGGCT AATTTTTCCA CCCTGCTGTG CTACGATCGG 
ATCTTCTGTG ACATTACCTA CTCGGTGACC TCTTGCACGG AGGGCGAGGC GACACGCTAT 
GGCCGCTTTC TGTGCGCCAT GCTGGAGACT GTGATGCGCT GGCATGCCGA TCAGGCCGTT 
TTTAACAAGG AGTGTGCCAA TTATCCCGGC TTTGTGACCA AATTTCGTGT CAGCAATCAG 
TTCTCAGAGG CCAACGATCA CGTGGGCTAC GAAAACTATC GACATGTGTG CCACAAGTGG 
CACTATAAGA TCACCAAGGC CATTGTTTTC TGTCTAGACT CGAAGGACTT TATGCAGATA 
CGGAATGCAC TGATCATTCT CATGCGTATA CTTCCACACT ATCCCGTGCT GGCCAAGCTG 
GCGCAGATCA TTGAACGGAA GGTGGACAAA GTGCGCGAAG AGGAGAAGAC GAAACGACCT 
GATCTGTATG TCATAGCCTC CAGCTATATT GGCAAACTGA AGCTGAAGAC ACCGAACATG 
TTCAAGGAGA GTGACTTTCA TCAGTTGCCT GAGCGGTTGC CCACTAAAGA TTCGCAGGAG 
CCGGAAGCCC CAGGCACTGG TAGTGGGCCC AACATCAAAG TGTTGGCCTC GGATAAGGTG 
GCAACACAAG GTCCTACCTC ACCAACGGCC ACCAAGTCGG GGGGCAGTGC GACGTCCTTC 
TACAACAGCG AACAAAATGC AACCGGCAAA GAACGAGATG TAAAGTCATC TCTCAGCACG 
CGCCACGACT CGAAACCCAC CGATCCCCGG GTGCCACCAT CATCCACCGC CAACTCAGCG 
CCGTCCGAAC GGGAGAGCAA GCAAAGAGAC CTGCCTGGAG CCAGAGAGTC ACACTCCCGC 
CTCACGAAGG ATGAGCTGCA TATGCATCAG GAGCAGCAGT TCCAGTCTGG CAGCATCAAT 
GCCAGCAATG GCAGCAGCCA AAACGATCGT CACAGCCGTG AAGTGGATCG TGAACGGGAG 
CACGAGCAGA GGCACAGCAG CCTCACCAGC AGCCATCGCA GCTCCCGAGA GACCAACAAT 
CGCCCCAAGG AGCGTACCGA AGCGGAGCTG AATCAGCGTG CCCGAGAGCG TGCCCAACGC 
CAGGAAGAAC TGGAGGCGGC AGCAGCCCAA CGTAATCAGA AGCGAGAGAA GACATCGCGA 
CGTGAGGAGC GGCATCGACA CGAGGGTCTG GAAACAGTGG ATCTGGTGGC CAGCAACTCA 
ACTGAGCATC GTCAGTTTGA CGAGTACGAG GGCCGCCATC GGGATCGTGA TCTGAGCTCT 
GTTTCGAACG AGAGCAATGG GTCCATGCAA CACAGACGCA GCCTTGAGGC TATTGAATAC 
GAAAAAGATT CAAAGCGTCG CAAATTGGAA TCATCGTCGA GCAGTTCAAA GAAGGTTGAG 
GAGCTCGTCG ACAGCGTGAA GAAGGCGCGT CTCAAAACCA AAGAGCGCAA CAAGGACAAG 
CTGTCCGATG AGGAACGGGA TGCCCGCAAG GATCGCAAGC TGGGACGCAA GCGGGACCGC 
GCCGAGGAGT CCAACAATAT CGAGCACAAG CGACGGCGCG AAGCACAAAA CGGCGATGAT 
GATCTGCGTG AGCGAGAGCG ACACAGAGAT AAATCCCCAC GAGAGCGTTC CCATGAGAAG 
TTTGACAGAG AACGAGGTGG AGGAGCCGCG GGCGGCAGTA GTTCGCGAGG CGACGATCGA 
CAGTACTCTA TCAAAACAAC ACGCTCCTCC AGGGTCAACT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001356647.2
CDS	112..5076
Translation	MRRLKKLSKSHSVTVTPESDGSSATTAMETATVAAASTASDTNGGSGAVSVLNPDIFKHFDKAGRSEFLKYLKQQLQESADSPVYDQNGVFRQGVSNAIYELLWQALRFTHKKDLILHLLTEVVAIHKDFPSLIVDVVNILDSETSLMTEGLQEERHGFVQLVKDMDKVVPESLLKERLEIDTLQEVGIVKNKGFYSKFIKVKTKLYYKQRRFNLFREESEGFAKLITELNQDFEESTTPESIMDIIKSLIGCFNLDPNRVLDIIIESFETRPDRWQLFIPLLRSYMPTGAIICEVLGYKFCHFKDSRTPRSLYHVCALLLKHEVIELNDVYVWLTPNDGSIKANWEHELSDAREMVRKLNLISTNKKEDDKEPPPPPPTVKRFDEEKYNLNQKFGLCEALLKVGDWENAHRIIQKLPEQSVVIQEPIARAIVELIHLSVEHVYYKKCFKAPAGRKQSRNRLYDDAKLVAKMQAREFVDLRKYTWPMANVLGPAMHCDTVLMYKLIRIMSKLIVEMGVDSMNGPPPNSEAEKHYYDIMTCMDSCILPSLLYLDNNCSMSEEIWAVLKYFPYHFRYSLYARWKNDSYQLHPNLIRRSGLAMRDIKGLMKRVSKENVKQLGRLVGKYCHCAPGLMFDYILLQIQMYDNLIAPVCDMLKFLTSLSFDCLGYCIIESLTLTGRGRFKDDGTSLSLWLQSLASFCGTIYKKYSIELSGLLQYVANQLKSQKSLDLLILREIVHKMAGVESCEEMTNEQLQALCGGEQLRGEAGYFSQVRNTKKSSNRLKEALANNDLAVAICLLMAQQKHCVIYRETAAHSHLKLVGNLYDQCQDTLVQFGTFLGSTYSVDEYVERLPSIITMLREYHINTDVAFFLARPMFTHQINQKYDQLRKSDPNAKKLTTTQKMQKYLDATQLIMNPIVESVRPLHSAKVWEDISPQFLVTFWSLSMYDLHVPNDSYQREIGKLKQLAHQAAEGKDSNQSKNKKEQERYIALMEKLNDERKKQHEHVDKIQQRLQEQRDSWFLLRSAKSAKNDTITQFLQLCLFPRCTFTALDALYCAKFVHTIHNLKTANFSTLLCYDRIFCDITYSVTSCTEGEATRYGRFLCAMLETVMRWHADQAVFNKECANYPGFVTKFRVSNQFSEANDHVGYENYRHVCHKWHYKITKAIVFCLDSKDFMQIRNALIILMRILPHYPVLAKLAQIIERKVDKVREEEKTKRPDLYVIASSYIGKLKLKTPNMFKESDFHQLPERLPTKDSQEPEAPGTGSGPNIKVLASDKVATQGPTSPTATKSGGSATSFYNSEQNATGKERDVKSSLSTRHDSKPTDPRVPPSSTANSAPSERESKQRDLPGARESHSRLTKDELHMHQEQQFQSGSINASNGSSQNDRHSREVDREREHEQRHSSLTSSHRSSRETNNRPKERTEAELNQRARERAQRQEELEAAAAQRNQKREKTSRREERHRHEGLETVDLVASNSTEHRQFDEYEGRHRDRDLSSVSNESNGSMQHRRSLEAIEYEKDSKRRKLESSSSSSKKVEELVDSVKKARLKTKERNKDKLSDEERDARKDRKLGRKRDRAEESNNIEHKRRREAQNGDDDLRERERHRDKSPRERSHEKFDRERGGGAAGGSSSRGDDRQYSIKTTRSSRVNY

Target ORF information:

RefSeq Version	XM_001356611.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA16382), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001356611.3

ORF Insert Sequence:

ATGCGGCGCT TGAAGAAATT GTCGAAGAGT CACAGCGTGA CGGTTACACC GGAGAGCGAT 
GGCAGCAGTG CGACGACGGC AATGGAGACG GCGACGGTAG CGGCTGCTTC GACGGCGTCC 
GACACAAATG GAGGGTCCGG CGCAGTCAGT GTCCTGAATC CAGATATATT TAAGCATTTC 
GACAAGGCCG GCAGAAGTGA ATTTTTGAAA TACCTCAAAC AACAGCTGCA AGAAAGTGCC 
GACAGTCCCG TATACGATCA GAATGGAGTC TTCAGGCAGG GCGTCTCGAA TGCCATATAC 
GAGCTGCTCT GGCAAGCATT GCGCTTCACC CACAAGAAGG ATCTTATCCT TCATCTGCTC 
ACAGAAGTTG TGGCCATACA TAAGGATTTC CCTAGTCTCA TTGTGGATGT GGTCAATATT 
TTGGACAGCG AGACTTCCCT TATGACAGAG GGGCTGCAAG AGGAGCGTCA TGGTTTTGTG 
CAGCTCGTCA AGGATATGGA CAAGGTGGTG CCAGAGAGTT TGCTCAAGGA GCGCCTGGAG 
ATTGATACGC TGCAGGAGGT GGGGATTGTG AAGAACAAGG GTTTCTACTC CAAGTTCATC 
AAGGTCAAAA CCAAGTTGTA CTACAAGCAG CGACGCTTTA ATCTATTCCG CGAGGAAAGC 
GAAGGCTTTG CCAAGCTGAT AACCGAGCTG AATCAGGACT TTGAGGAGAG CACCACTCCC 
GAGAGCATTA TGGACATTAT AAAATCGCTG ATAGGTTGCT TCAATCTGGA TCCCAATCGC 
GTGCTGGATA TCATAATTGA ATCGTTTGAA ACCCGCCCGG ATCGTTGGCA ATTGTTTATA 
CCCTTGCTAC GCAGCTATAT GCCCACGGGT GCCATTATTT GTGAAGTACT CGGCTACAAG 
TTCTGCCATT TTAAGGACTC TCGTACGCCC CGATCTCTGT ACCATGTGTG CGCGCTGCTG 
TTGAAGCACG AGGTGATTGA ACTGAACGAT GTGTACGTCT GGTTGACCCC CAATGATGGC 
AGCATCAAGG CCAACTGGGA GCACGAGCTG TCGGATGCCC GAGAAATGGT GCGAAAACTG 
AATCTGATCT CCACCAACAA AAAGGAAGAC GACAAAGAGC CACCACCACC GCCGCCAACC 
GTTAAGCGGT TTGATGAGGA AAAGTACAAT CTAAATCAAA AGTTTGGTCT CTGCGAGGCT 
CTGCTCAAGG TGGGGGACTG GGAGAATGCC CACAGAATCA TACAAAAACT GCCGGAACAA 
TCGGTGGTCA TACAAGAGCC CATTGCCCGT GCCATTGTCG AACTGATTCA TTTGTCCGTG 
GAGCATGTCT ACTACAAGAA ATGCTTCAAA GCCCCCGCCG GACGCAAACA GAGTCGCAAT 
CGTTTGTACG ATGATGCCAA GCTGGTGGCC AAGATGCAGG CCCGTGAATT TGTTGATCTG 
CGGAAGTATA CGTGGCCGAT GGCTAATGTT TTGGGCCCGG CGATGCACTG TGACACGGTG 
CTAATGTACA AACTGATACG GATTATGAGC AAGCTAATTG TGGAGATGGG TGTCGACAGC 
ATGAATGGCC CGCCGCCTAA TTCAGAGGCG GAGAAGCATT ACTACGACAT AATGACCTGC 
ATGGATTCGT GCATACTGCC ATCGCTGCTG TATTTGGACA ATAATTGTTC CATGTCCGAG 
GAGATTTGGG CTGTGCTCAA GTATTTCCCG TATCATTTTC GATACAGTTT GTATGCCAGA 
TGGAAAAACG ATAGCTACCA GCTGCATCCG AATCTGATAC GACGCAGTGG CTTGGCCATG 
CGCGACATCA AGGGACTGAT GAAGCGTGTC AGCAAGGAAA ATGTAAAGCA ATTGGGTCGA 
CTGGTGGGCA AGTACTGTCA CTGTGCCCCT GGACTGATGT TTGATTATAT TCTCCTGCAA 
ATCCAGATGT ACGACAACCT TATCGCACCT GTCTGTGACA TGTTGAAGTT CCTGACATCT 
CTGTCCTTTG ATTGCCTGGG CTATTGCATC ATTGAATCGC TGACTCTGAC GGGTCGTGGC 
AGGTTCAAAG ACGACGGCAC TTCGCTTTCC CTGTGGCTGC AAAGTCTGGC CTCCTTTTGT 
GGGACCATCT ACAAAAAATA CAGCATCGAA TTGAGCGGCC TGCTGCAGTA TGTGGCCAAT 
CAATTGAAGT CACAGAAGAG CCTGGATCTG CTAATACTAC GCGAGATTGT GCACAAGATG 
GCTGGCGTGG AGTCGTGCGA GGAGATGACC AACGAACAGT TGCAGGCCTT GTGCGGGGGC 
GAACAGCTGC GCGGCGAGGC GGGATATTTC AGTCAAGTGA GGAACACTAA GAAGTCATCA 
AATCGGCTGA AGGAGGCCCT GGCCAACAAT GATCTGGCTG TGGCCATTTG TCTGCTGATG 
GCCCAGCAGA AGCATTGTGT GATCTATCGT GAAACGGCGG CGCATAGTCA TCTGAAGCTG 
GTGGGGAATC TTTATGATCA GTGCCAGGAC ACGCTAGTGC AATTTGGCAC CTTTTTGGGT 
TCCACCTATT CGGTGGATGA GTATGTGGAG CGACTGCCAT CGATCATAAC GATGCTAAGG 
GAGTATCACA TCAATACGGA TGTGGCATTC TTTCTGGCGA GGCCCATGTT TACACATCAG 
ATTAATCAAA AGTACGATCA ATTGCGTAAA TCCGATCCGA ATGCCAAAAA ACTGACCACC 
ACACAGAAAA TGCAAAAGTA TCTGGACGCC ACACAACTCA TCATGAACCC CATTGTGGAG 
TCTGTGCGTC CGCTGCATAG TGCCAAAGTG TGGGAGGACA TAAGTCCGCA GTTTTTGGTG 
ACCTTCTGGA GCCTCAGCAT GTACGATCTG CATGTCCCCA ACGATAGCTA CCAGCGTGAG 
ATTGGAAAAC TCAAGCAATT GGCCCATCAG GCGGCCGAGG GCAAGGACTC CAACCAGTCC 
AAGAATAAAA AAGAACAAGA GCGATACATT GCTTTGATGG AGAAGCTAAA TGATGAGCGG 
AAGAAGCAAC ACGAGCATGT GGACAAGATA CAGCAGCGTC TGCAAGAGCA GAGGGACAGC 
TGGTTCCTGC TGCGTTCGGC CAAGTCCGCC AAGAACGATA CCATTACGCA GTTCCTGCAA 
TTGTGCCTGT TTCCGCGCTG CACTTTCACT GCCCTGGATG CGCTCTATTG CGCAAAGTTT 
GTCCATACCA TCCACAATCT AAAGACGGCT AATTTTTCCA CCCTGCTGTG CTACGATCGG 
ATCTTCTGTG ACATTACCTA CTCGGTGACC TCTTGCACGG AGGGCGAGGC GACACGCTAT 
GGCCGCTTTC TGTGCGCCAT GCTGGAGACT GTGATGCGCT GGCATGCCGA TCAGGCCGTT 
TTTAACAAGG AGTGTGCCAA TTATCCCGGC TTTGTGACCA AATTTCGTGT CAGCAATCAG 
TTCTCAGAGG CCAACGATCA CGTGGGCTAC GAAAACTATC GACATGTGTG CCACAAGTGG 
CACTATAAGA TCACCAAGGC CATTGTTTTC TGTCTAGACT CGAAGGACTT TATGCAGATA 
CGGAATGCAC TGATCATTCT CATGCGTATA CTTCCACACT ATCCCGTGCT GGCCAAGCTG 
GCGCAGATCA TTGAACGGAA GGTGGACAAA GTGCGCGAAG AGGAGAAGAC GAAACGACCT 
GATCTGTATG TCATAGCCTC CAGCTATATT GGCAAACTGA AGCTGAAGAC ACCGAACATG 
TTCAAGGAGA GTGACTTTCA TCAGTTGCCT GAGCGGTTGC CCACTAAAGA TTCGCAGGAG 
CCGGAAGCCC CAGGCACTGG TAGTGGGCCC AACATCAAAG TGTTGGCCTC GGATAAGGTG 
GCAACACAAG GTCCTACCTC ACCAACGGCC ACCAAGTCGG GGGGCAGTGC GACGTCCTTC 
TACAACAGCG AACAAAATGC AACCGGCAAA GAACGAGATG TAAAGTCATC TCTCAGCACG 
CGCCACGACT CGAAACCCAC CGATCCCCGG GTGCCACCAT CATCCACCGC CAACTCAGCG 
CCGTCCGAAC GGGAGAGCAA GCAAAGAGAC CTGCCTGGAG CCAGAGAGTC ACACTCCCGC 
CTCACGAAGG ATGAGCTGCA TATGCATCAG GAGCAGCAGT TCCAGTCTGG CAGCATCAAT 
GCCAGCAATG GCAGCAGCCA AAACGATCGT CACAGCCGTG AAGTGGATCG TGAACGGGAG 
CACGAGCAGA GGCACAGCAG CCTCACCAGC AGCCATCGCA GCTCCCGAGA GACCAACAAT 
CGCCCCAAGG AGCGTACCGA AGCGGAGCTG AATCAGCGTG CCCGAGAGCG TGCCCAACGC 
CAGGAAGAAC TGGAGGCGGC AGCAGCCCAA CGTAATCAGA AGCGAGAGAA GACATCGCGA 
CGTGAGGAGC GGCATCGACA CGAGGGTCTG GAAACAGTGG ATCTGGTGGC CAGCAACTCA 
ACTGAGCATC GTCAGTTTGA CGAGTACGAG GGCCGCCATC GGGATCGTGA TCTGAGCTCT 
GTTTCGAACG AGAGCAATGG GTCCATGCAA CACAGACGCA GCCTTGAGGC TATTGAATAC 
GAAAAAGATT CAAAGCGTCG CAAATTGGAA TCATCGTCGA GCAGTTCAAA GAAGGTTGAG 
GAGCTCGTCG ACAGCGTGAA GAAGGCGCGT CTCAAAACCA AAGAGCGCAA CAAGGACAAG 
CTGTCCGATG AGGAACGGGA TGCCCGCAAG GATCGCAAGC TGGGACGCAA GCGGGACCGC 
GCCGAGGAGT CCAACAATAT CGAGCACAAG CGACGGCGCG AAGCACAAAA CGGCGATGAT 
GATCTGCGTG AGCGAGAGCG ACACAGAGAT AAATCCCCAC GAGAGCGTTC CCATGAGAAG 
TTTGACAGAG AACGAGGTGG AGGAGCCGCG GGCGGCAGTA GTTCGCGAGG CGACGATCGA 
CAGTACTCTA TCAAAACAAC ACGCTCCTCC AGGGTCAACT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA16382 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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