Dpse\GA18678 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA18678
Entrez Gene ID	4817001
Full Name	GA18678 gene product from transcript GA18678-RA
Synonyms	GA18678,dpse_GLEANR_2179
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001356358.3	XP_001356394.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA18678 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA18678 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe15522	XM_001356358.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA18678), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA18678 ( XM_001356358.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001356394.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA18678), mRNA.

Price & Availability↑

CloneID	ODe15522
Clone ID Related Accession (Same CDS sequence)	XM_001356358.3
Accession Version	XM_001356358.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589964). On Dec 22, 2015 this sequence version replaced XM_001356358.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATTTAC CGTATTCGCA ATTGCCGGAT CCAACCGATA AGTTTGAGAT CTATGAAGAA 
ATAGCCCAGG GTGTCAATGC CAAAGTATTT CGGGCCAAGG AATTGGAGAC CGATCGTCTG 
GTGGCATTAA AGATACAGCA CTACGATGAG GAGCATCAGG TGTCCATCGA GGAGGAGTAT 
CGTACTCTGC GCGACTATTG TGCCCACCCC AATCTCCCGG AGTTCTATGG CGTGTATAAG 
CTATCGAAGC CGAATGGTCC TGATGAGATT TGGTTCGTTT TGGAGTACTG TGCTGGAGGA 
ACAGCCGTAG ATATGGTTAA TAAGCTCCTG AAACTGGACC GTAGGATGAG AGAGGAGCAC 
ATCGCCTACA TCATCCGGGA GACGTGTCGG GCAGCCATCG AGCTGAATCG CAATCATGTC 
CTGCATCGGG ACATCCGTGG CGACAACATT TTGCTCACGA AAAATGGACG GGTGAAGCTC 
TGCGATTTCG GTCTGTCCCG TCAAGTGGAT TCGACTTTGG GGAAGCGAGG CACTTGCATT 
GGTTCGCCGT GTTGGATGGC CCCCGAAGTG GTGGCTGCCA TGGAGTCCCG CGAACCCGAT 
ATCACCGTTC GGGCAGATGT CTGGGCCCTG GGTATCACCA CCATTGAGTT GGCCGATGGA 
AAGCCTCCCT TTGCTGATAT GCATCCCACT CGAGCCATGT TCCAGATCGT GCGGAATCCA 
CCGCCGACAC TGATGCGACC CACGAATTGG TCCCAGCAGA TCAATGACTT TATTGCCGAG 
TGTCTGGAGA AGAGTGCCGA GAATCGCCCC ATGATGGTCG AGATGGTAGA GCATCCATTC 
CTCACGGAGC TCATTGAGAA CGAGCAGGAG ATGCAGTCGG ATCTTGCAGA GATGCTGGAG 
CTTTCGCGGG ACGTGAAGAG CCTGTACAAG GAGCCGGAAC TATTTGTGGA TCGCGGCTAT 
ATCAAGCGCT TCGATGGGAA GCCGGAGCGC ATGTATCCTG AAGATTTGGC AGCCCTGGAA 
AACCCCGTGG ATGAGAGCAT CATTGATTCG CTGCTAAATC GCATGATGAC TGGTGAATCC 
TATAGTTTTA TTGGAGATAT CCTGCTGTCC CTGAATTCGA ATGAGATCCA AAGCGAATTC 
CCTCCGGAGT TCCACGCCAA ATACCGATTC AAGTCGCGCT CGGAGAACCA ACCCCATATC 
TTCTCCGTGG CCGATATTGC CTACCAGGAT ATGCTCCATC ATCGCGAGCC CCAGCACATT 
GTCCTTTCTG GGGAGAGCTA CTCGGGCAAG TCGACCAACG CCCGTCTGCT GATCAAGCAT 
CTGTGCTATC TTGGGGCTGG CAATCGGGGT GCCACTGGGC GCGTGGAGAC CTCCATTAAG 
GCCATCCTGA TGATGGTCAA TGCCGGCACT CCCGTCAACA ACGACTCCAC CCGCTGTGTC 
CTGCAGTATT GTCTGACTTT CGGCAAGACG GGAAAGATGA GTGGAGCGGT ATTCAATATG 
TATATGCTGG AGAAGCTGCG AGTGGCCACG TGTGACGGCA CCCAGCACAA TTTCCACATT 
TTCTACTATT TCTATGACTT CATCAACCAG CAGAATCAGC TGAAGGACTA CAATCTGAAA 
GCGGACCGCA ACTATCGCTA TCTGCGCGTG CCACCAGATG CTCCCCCAAC CAAGATCAAG 
TATCGTCGCG ACGATCCCGA GGGTAATGTG GAGCACTACC GTGAGTTCGA GAACATTCTC 
AGGGATCTGG ACTTTAACCA CAAGCAGCTG GAGACTGTGC GAAAGGTGCT GGCTGCCATC 
TTAAACATTG GAAACATTCG CTTCCGACAG TCCGGCAAGT ACGCCGAAGT CGAGAACACG 
GATATAGTAT CGCGTATTGC CGAGCTCTTG AGGGTGGATG AGAAGAAATT CATGTGGTCC 
TTAACAAACT TCATAATGGT CAAGGGAGGT GTGGCCGAAA GACGGCAGTA TACCGCAGAG 
GAGGCTCGCG ATGCACGCGA TGCTGTGGCC AGCACCATCT ACTCCCGATT TGTGGACTTT 
ATTATCAACA GGATTAACAT GAACATGTCG TTTCCAAGAG CTGTCTTTGG CGACACAAAC 
GCCATCATTA TCCATGACAT GTTCGGCTTT GAGTGCTTCA ATCGGAATGG CATGGAGCAG 
CTAATGATCA ACACTCTGAA CGAACAGATG CAATATCACT ACAACCAACG CATTTTTATT 
AGCGAAATGC TCGAAATGGA GGCCGAGGAT ATAGACACCA TTAACTTGAA CTTCTACGAT 
AACAAGACCG CTTTGGATAA TCTGCTAACC AAGCCAGATG GTCTCTTCTA CATCATTGAT 
GATGCCTCAC GCTCCTGCCA GGATCAGGAC TTGATTATGG ATCGCGTTTC GGAGAAACAC 
AGCCAGTTTG TGAAGAAGCA CACTGCCACT GAAATATCCG TCGCTCATTA TACGGGCCGC 
ATCATCTATG ATACTCGCTC CTTCACGGAC ATCAACCGCG ACTTTGTCCC CCCGGAAATG 
ATTGAGACGT TCCGCTCTTC GCTGGACGAG AGCATAATGC TCATGTTCAC CAATCAACTG 
ACGAAAGCTG GCAATCTGAC AATGCCTTTT GAGGCTGTCC AGCATAAGGA TGAAACTGAA 
CGCAGGTCCT ACGCCCTCAA CACTTTGAGT GCTGGATGCA TCTCCCAGGT GAACAATTTA 
CGTACTCTGT CGGCCAATTT CCGCTTCACC TGCCTGACTC TGCTCAAAAT GCTAAGCCAA 
AATGCCAGTC TCGGCGTGCA CTTTGTCCGT TGCATACGAG CTGATTTGGA GTACAAGCCG 
CGTGCGTTCC ACTCGGACGT GGTGCAGCAA CAGATGAAGG CTCTGGGTGT CCTGGACACG 
GTCATTGCCC GGCAAAAGGG CTACAGCTCT CGACTGGGCT TCGATGAGTT CCTCAGACGC 
TATCAATTCC TAGCCTTTGA CTTTGATGAG CCTGTGGAAA TGACAAAAGA GAATTGTCGT 
CTCTTATTTT TACGTCTCAA GATGGAGGGT TGGGCCTTGG GCAAGTCCAA AGTCTTTTTG 
CGCTACTACA ACGATGAGTT CTTAGCCAGA AATTACGAGC TTCAAGTGAA GAAAGTCATC 
AAAGTGCAGT CCATGATGCG GGCACTTTTA GCCCGAAAAC GTGTCAAAGG TGGCAAGGTC 
TTTAAATTGG GCAAAAAGGG TCAGGAGCAT TCCGATTTGG CGGCTGCCAA AATACAAAAA 
GCGTATCGCG AGTTCCGTGA CCGGGTCCGT CTGCCGCCGC TGGTCAATGA GAAGACGGGC 
CAACGGAATG AGAACACCGC CGACTTTATT CGACCCTATG CCAAGAAATG GCGAGAGAAG 
TCCATCTTTC AGGTCCTGCT GCACTACAGA GCGGCCCGCT TCCAGGACTT TGTCAATCTC 
TCACAACAGG TACATATTTA CAACCAACGG ATGGTCGGTG GGCTGAACAA ATGCACCCGG 
TCGGTGCCCT TCGAGCGCAT TAATATGCGG GAGGTAAACT CCTCGCAGCT GGGACCGCTC 
CCTGTTCCGA TGAAGAAGAT GCCATTCCGC CTGGATCAGA TACCCTTCTA TGACACACAG 
TACATGGTGG ATCCGGCCAA TTCTATATCT CGTCAAACGT TTCCCAATCA GTTGCTGGTG 
CAAAATCTGG AAGATGATGA AGCCTGGGAT AGTCCTCTAC AGCGCAATCC TTCGATGACT 
TCGTGTGCCA TGACCTACAA TGCTTATAAG AAAGAACAGG CTTGCCAGAC CAATTGGGAT 
CGTATGGGCG AAAGCGATAA TATATACAAT CAGGGGTTCT TTAGAGATCC ACAGCAGTTA 
AGAAGAAACC AAGTGCAACA AAACATGAAT GCTTACAACA ATGCCTATAA CAACTACAAC 
AGCAGCAACA ATCCCAACTG GGGCCGGTCA GGATCCCGTC GTAATTCCCT CAAGGGCTAC 
GCGGCGCCAC CGCCGCCTCC ACCGATGCCT TCATCCAACA ACTACTATCG AAATAATCCC 
AGTCAACAGC CTCAAAGGAA CTACCAGCAA CGCTCCTCGT ATCCACCTTC AGATCCCATA 
AGAGAGCTCC AAAACATGGC ACGCAACGAC GGAGACAACT CCGAGGATCC TCCATTCAAC 
TTCAAGGCCA TGCTGCGCAA GACAAACTAT CCAAGGGGCT CAGAGACCAG CACCTATGAC 
TTTAACAATC GCCGGGGGTC GGACGCCGGG GATCAGCAGC ACACATTCCA GGCACCCAAA 
CTACGCTCCA CTGGACGCCG CTTCGAAGAT GAGGGGGGCT ATAAAACCTC GGCGGGCAAC 
TATGGGGCAG CCCGCAACTT TGGCCAGCAG CGGGCTCCTC CTTTGAACCA GAGCTCCTCC 
AGTGTGGGTC GCAGCTTTGA GAACAGCAAT GCAAGGTCCT TCGAGCAGGC GGGCTCCTAT 
GTTGAAGAGG AGATTGCACC AGGGGTGACC CTATCCGGAT ATGCAGTGGA CATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001356394.1
CDS	152..4648
Translation	MYLPYSQLPDPTDKFEIYEEIAQGVNAKVFRAKELETDRLVALKIQHYDEEHQVSIEEEYRTLRDYCAHPNLPEFYGVYKLSKPNGPDEIWFVLEYCAGGTAVDMVNKLLKLDRRMREEHIAYIIRETCRAAIELNRNHVLHRDIRGDNILLTKNGRVKLCDFGLSRQVDSTLGKRGTCIGSPCWMAPEVVAAMESREPDITVRADVWALGITTIELADGKPPFADMHPTRAMFQIVRNPPPTLMRPTNWSQQINDFIAECLEKSAENRPMMVEMVEHPFLTELIENEQEMQSDLAEMLELSRDVKSLYKEPELFVDRGYIKRFDGKPERMYPEDLAALENPVDESIIDSLLNRMMTGESYSFIGDILLSLNSNEIQSEFPPEFHAKYRFKSRSENQPHIFSVADIAYQDMLHHREPQHIVLSGESYSGKSTNARLLIKHLCYLGAGNRGATGRVETSIKAILMMVNAGTPVNNDSTRCVLQYCLTFGKTGKMSGAVFNMYMLEKLRVATCDGTQHNFHIFYYFYDFINQQNQLKDYNLKADRNYRYLRVPPDAPPTKIKYRRDDPEGNVEHYREFENILRDLDFNHKQLETVRKVLAAILNIGNIRFRQSGKYAEVENTDIVSRIAELLRVDEKKFMWSLTNFIMVKGGVAERRQYTAEEARDARDAVASTIYSRFVDFIINRINMNMSFPRAVFGDTNAIIIHDMFGFECFNRNGMEQLMINTLNEQMQYHYNQRIFISEMLEMEAEDIDTINLNFYDNKTALDNLLTKPDGLFYIIDDASRSCQDQDLIMDRVSEKHSQFVKKHTATEISVAHYTGRIIYDTRSFTDINRDFVPPEMIETFRSSLDESIMLMFTNQLTKAGNLTMPFEAVQHKDETERRSYALNTLSAGCISQVNNLRTLSANFRFTCLTLLKMLSQNASLGVHFVRCIRADLEYKPRAFHSDVVQQQMKALGVLDTVIARQKGYSSRLGFDEFLRRYQFLAFDFDEPVEMTKENCRLLFLRLKMEGWALGKSKVFLRYYNDEFLARNYELQVKKVIKVQSMMRALLARKRVKGGKVFKLGKKGQEHSDLAAAKIQKAYREFRDRVRLPPLVNEKTGQRNENTADFIRPYAKKWREKSIFQVLLHYRAARFQDFVNLSQQVHIYNQRMVGGLNKCTRSVPFERINMREVNSSQLGPLPVPMKKMPFRLDQIPFYDTQYMVDPANSISRQTFPNQLLVQNLEDDEAWDSPLQRNPSMTSCAMTYNAYKKEQACQTNWDRMGESDNIYNQGFFRDPQQLRRNQVQQNMNAYNNAYNNYNSSNNPNWGRSGSRRNSLKGYAAPPPPPPMPSSNNYYRNNPSQQPQRNYQQRSSYPPSDPIRELQNMARNDGDNSEDPPFNFKAMLRKTNYPRGSETSTYDFNNRRGSDAGDQQHTFQAPKLRSTGRRFEDEGGYKTSAGNYGAARNFGQQRAPPLNQSSSSVGRSFENSNARSFEQAGSYVEEEIAPGVTLSGYAVDI

Target ORF information:

RefSeq Version	XM_001356358.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA18678), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001356358.3

ORF Insert Sequence:

ATGTATTTAC CGTATTCGCA ATTGCCGGAT CCAACCGATA AGTTTGAGAT CTATGAAGAA 
ATAGCCCAGG GTGTCAATGC CAAAGTATTT CGGGCCAAGG AATTGGAGAC CGATCGTCTG 
GTGGCATTAA AGATACAGCA CTACGATGAG GAGCATCAGG TGTCCATCGA GGAGGAGTAT 
CGTACTCTGC GCGACTATTG TGCCCACCCC AATCTCCCGG AGTTCTATGG CGTGTATAAG 
CTATCGAAGC CGAATGGTCC TGATGAGATT TGGTTCGTTT TGGAGTACTG TGCTGGAGGA 
ACAGCCGTAG ATATGGTTAA TAAGCTCCTG AAACTGGACC GTAGGATGAG AGAGGAGCAC 
ATCGCCTACA TCATCCGGGA GACGTGTCGG GCAGCCATCG AGCTGAATCG CAATCATGTC 
CTGCATCGGG ACATCCGTGG CGACAACATT TTGCTCACGA AAAATGGACG GGTGAAGCTC 
TGCGATTTCG GTCTGTCCCG TCAAGTGGAT TCGACTTTGG GGAAGCGAGG CACTTGCATT 
GGTTCGCCGT GTTGGATGGC CCCCGAAGTG GTGGCTGCCA TGGAGTCCCG CGAACCCGAT 
ATCACCGTTC GGGCAGATGT CTGGGCCCTG GGTATCACCA CCATTGAGTT GGCCGATGGA 
AAGCCTCCCT TTGCTGATAT GCATCCCACT CGAGCCATGT TCCAGATCGT GCGGAATCCA 
CCGCCGACAC TGATGCGACC CACGAATTGG TCCCAGCAGA TCAATGACTT TATTGCCGAG 
TGTCTGGAGA AGAGTGCCGA GAATCGCCCC ATGATGGTCG AGATGGTAGA GCATCCATTC 
CTCACGGAGC TCATTGAGAA CGAGCAGGAG ATGCAGTCGG ATCTTGCAGA GATGCTGGAG 
CTTTCGCGGG ACGTGAAGAG CCTGTACAAG GAGCCGGAAC TATTTGTGGA TCGCGGCTAT 
ATCAAGCGCT TCGATGGGAA GCCGGAGCGC ATGTATCCTG AAGATTTGGC AGCCCTGGAA 
AACCCCGTGG ATGAGAGCAT CATTGATTCG CTGCTAAATC GCATGATGAC TGGTGAATCC 
TATAGTTTTA TTGGAGATAT CCTGCTGTCC CTGAATTCGA ATGAGATCCA AAGCGAATTC 
CCTCCGGAGT TCCACGCCAA ATACCGATTC AAGTCGCGCT CGGAGAACCA ACCCCATATC 
TTCTCCGTGG CCGATATTGC CTACCAGGAT ATGCTCCATC ATCGCGAGCC CCAGCACATT 
GTCCTTTCTG GGGAGAGCTA CTCGGGCAAG TCGACCAACG CCCGTCTGCT GATCAAGCAT 
CTGTGCTATC TTGGGGCTGG CAATCGGGGT GCCACTGGGC GCGTGGAGAC CTCCATTAAG 
GCCATCCTGA TGATGGTCAA TGCCGGCACT CCCGTCAACA ACGACTCCAC CCGCTGTGTC 
CTGCAGTATT GTCTGACTTT CGGCAAGACG GGAAAGATGA GTGGAGCGGT ATTCAATATG 
TATATGCTGG AGAAGCTGCG AGTGGCCACG TGTGACGGCA CCCAGCACAA TTTCCACATT 
TTCTACTATT TCTATGACTT CATCAACCAG CAGAATCAGC TGAAGGACTA CAATCTGAAA 
GCGGACCGCA ACTATCGCTA TCTGCGCGTG CCACCAGATG CTCCCCCAAC CAAGATCAAG 
TATCGTCGCG ACGATCCCGA GGGTAATGTG GAGCACTACC GTGAGTTCGA GAACATTCTC 
AGGGATCTGG ACTTTAACCA CAAGCAGCTG GAGACTGTGC GAAAGGTGCT GGCTGCCATC 
TTAAACATTG GAAACATTCG CTTCCGACAG TCCGGCAAGT ACGCCGAAGT CGAGAACACG 
GATATAGTAT CGCGTATTGC CGAGCTCTTG AGGGTGGATG AGAAGAAATT CATGTGGTCC 
TTAACAAACT TCATAATGGT CAAGGGAGGT GTGGCCGAAA GACGGCAGTA TACCGCAGAG 
GAGGCTCGCG ATGCACGCGA TGCTGTGGCC AGCACCATCT ACTCCCGATT TGTGGACTTT 
ATTATCAACA GGATTAACAT GAACATGTCG TTTCCAAGAG CTGTCTTTGG CGACACAAAC 
GCCATCATTA TCCATGACAT GTTCGGCTTT GAGTGCTTCA ATCGGAATGG CATGGAGCAG 
CTAATGATCA ACACTCTGAA CGAACAGATG CAATATCACT ACAACCAACG CATTTTTATT 
AGCGAAATGC TCGAAATGGA GGCCGAGGAT ATAGACACCA TTAACTTGAA CTTCTACGAT 
AACAAGACCG CTTTGGATAA TCTGCTAACC AAGCCAGATG GTCTCTTCTA CATCATTGAT 
GATGCCTCAC GCTCCTGCCA GGATCAGGAC TTGATTATGG ATCGCGTTTC GGAGAAACAC 
AGCCAGTTTG TGAAGAAGCA CACTGCCACT GAAATATCCG TCGCTCATTA TACGGGCCGC 
ATCATCTATG ATACTCGCTC CTTCACGGAC ATCAACCGCG ACTTTGTCCC CCCGGAAATG 
ATTGAGACGT TCCGCTCTTC GCTGGACGAG AGCATAATGC TCATGTTCAC CAATCAACTG 
ACGAAAGCTG GCAATCTGAC AATGCCTTTT GAGGCTGTCC AGCATAAGGA TGAAACTGAA 
CGCAGGTCCT ACGCCCTCAA CACTTTGAGT GCTGGATGCA TCTCCCAGGT GAACAATTTA 
CGTACTCTGT CGGCCAATTT CCGCTTCACC TGCCTGACTC TGCTCAAAAT GCTAAGCCAA 
AATGCCAGTC TCGGCGTGCA CTTTGTCCGT TGCATACGAG CTGATTTGGA GTACAAGCCG 
CGTGCGTTCC ACTCGGACGT GGTGCAGCAA CAGATGAAGG CTCTGGGTGT CCTGGACACG 
GTCATTGCCC GGCAAAAGGG CTACAGCTCT CGACTGGGCT TCGATGAGTT CCTCAGACGC 
TATCAATTCC TAGCCTTTGA CTTTGATGAG CCTGTGGAAA TGACAAAAGA GAATTGTCGT 
CTCTTATTTT TACGTCTCAA GATGGAGGGT TGGGCCTTGG GCAAGTCCAA AGTCTTTTTG 
CGCTACTACA ACGATGAGTT CTTAGCCAGA AATTACGAGC TTCAAGTGAA GAAAGTCATC 
AAAGTGCAGT CCATGATGCG GGCACTTTTA GCCCGAAAAC GTGTCAAAGG TGGCAAGGTC 
TTTAAATTGG GCAAAAAGGG TCAGGAGCAT TCCGATTTGG CGGCTGCCAA AATACAAAAA 
GCGTATCGCG AGTTCCGTGA CCGGGTCCGT CTGCCGCCGC TGGTCAATGA GAAGACGGGC 
CAACGGAATG AGAACACCGC CGACTTTATT CGACCCTATG CCAAGAAATG GCGAGAGAAG 
TCCATCTTTC AGGTCCTGCT GCACTACAGA GCGGCCCGCT TCCAGGACTT TGTCAATCTC 
TCACAACAGG TACATATTTA CAACCAACGG ATGGTCGGTG GGCTGAACAA ATGCACCCGG 
TCGGTGCCCT TCGAGCGCAT TAATATGCGG GAGGTAAACT CCTCGCAGCT GGGACCGCTC 
CCTGTTCCGA TGAAGAAGAT GCCATTCCGC CTGGATCAGA TACCCTTCTA TGACACACAG 
TACATGGTGG ATCCGGCCAA TTCTATATCT CGTCAAACGT TTCCCAATCA GTTGCTGGTG 
CAAAATCTGG AAGATGATGA AGCCTGGGAT AGTCCTCTAC AGCGCAATCC TTCGATGACT 
TCGTGTGCCA TGACCTACAA TGCTTATAAG AAAGAACAGG CTTGCCAGAC CAATTGGGAT 
CGTATGGGCG AAAGCGATAA TATATACAAT CAGGGGTTCT TTAGAGATCC ACAGCAGTTA 
AGAAGAAACC AAGTGCAACA AAACATGAAT GCTTACAACA ATGCCTATAA CAACTACAAC 
AGCAGCAACA ATCCCAACTG GGGCCGGTCA GGATCCCGTC GTAATTCCCT CAAGGGCTAC 
GCGGCGCCAC CGCCGCCTCC ACCGATGCCT TCATCCAACA ACTACTATCG AAATAATCCC 
AGTCAACAGC CTCAAAGGAA CTACCAGCAA CGCTCCTCGT ATCCACCTTC AGATCCCATA 
AGAGAGCTCC AAAACATGGC ACGCAACGAC GGAGACAACT CCGAGGATCC TCCATTCAAC 
TTCAAGGCCA TGCTGCGCAA GACAAACTAT CCAAGGGGCT CAGAGACCAG CACCTATGAC 
TTTAACAATC GCCGGGGGTC GGACGCCGGG GATCAGCAGC ACACATTCCA GGCACCCAAA 
CTACGCTCCA CTGGACGCCG CTTCGAAGAT GAGGGGGGCT ATAAAACCTC GGCGGGCAAC 
TATGGGGCAG CCCGCAACTT TGGCCAGCAG CGGGCTCCTC CTTTGAACCA GAGCTCCTCC 
AGTGTGGGTC GCAGCTTTGA GAACAGCAAT GCAAGGTCCT TCGAGCAGGC GGGCTCCTAT 
GTTGAAGAGG AGATTGCACC AGGGGTGACC CTATCCGGAT ATGCAGTGGA CATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA18678 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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