Dpse\GA10757 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA10757
Entrez Gene ID	4816181
Full Name	GA10757 gene product from transcript GA10757-RB
Synonyms	GA10757,dpse_GLEANR_7343
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001355758.3	XP_001355794.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA10757 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA10757 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe18775	XM_001355758.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10757), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA10757 ( XM_001355758.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001355794.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10757), mRNA.

Price & Availability↑

CloneID	ODe18775
Clone ID Related Accession (Same CDS sequence)	XM_001355758.3
Accession Version	XM_001355758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589962). On Dec 22, 2015 this sequence version replaced XM_001355758.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGCTGA CAAACGAGAA CTTGAAACTC CACCCAATGC TGAGGAATTT GGCCATTTTC 
CTCAGCCTAA CAATCTTCAT TTGTCTTCCA ACGACGTGGG CCGCAGAGAC GGCGACTCTC 
TCGGACAAAA GACTGTGCGC TGACCCAAAA TGTGAACAAA TCATCTCCTT GGGCACCACC 
AAGATACTGT ACAACTCTGG CGGCAAGGGA CTGATCTCCT TCAAAATCAA CACCCCAGTG 
CGCATCTTGT CCAAAAGTGC TGGCACGAAT AAGCAGCTCT GGGGCGTGGA TATCGGCGGA 
CGTCGTGGCT ATGCCAACAA GGATTTCATA TTGGAGAACA GGATCATGAT AACCGAGAAG 
GATTTAAAGT ATGAAGTGCC AGTGGTTGGA CCTGGTAGTC CACCACCCGC CGTGGCACCT 
GCTGCCACAC CACCAGAAGC TCCGCTGAAG GCGACAGAGG TAGAGCCGGC TGTACTTAAT 
GCCTCTGAGT CAGCCGATGA GTTGGCAACG ACTACAACCA GCCCGCTTGA TGTAAAAGAG 
GATTCCATTG TCACGAAAAA GGTTGAGGTG GATGACCAAC AGGTACCCGA TGTTCCAACC 
GCGTCATCTC AAGTCCTGCA GATGGTAGAC GGCACTCAGC TGCCAATGGA GGCAATTCCA 
GCTGTCACAG AGAGAACTCA CGATCAACAG GTCGTCCCAC CCACACAGAA ATCTCAAACA 
GCCTCAGCAG CGGTTGACAC GAAAGTGCCA CAACCGGTGG CCCCAACGAT TCAATCGAAT 
CAGTCACAGG AGCAGCAGCA GCAACTAGAG AAAGCAGTCA CTGACCAAAA TGAGCCGACG 
CCAGCAGTGA CGGAGCCTCA AAAGCTACAA CAGGTGGAAA TTGAACACAA AGACCCACAG 
GAGGCGGCAG CAACTGAAGC AGCGTCTGCT CCCAAGAAGT CGGAGCCAGA GCCAGTGACA 
GTGCCACCGG TGGCCATCAA CGCTGATATT GACGACGACT CTGATGATTT CGATTACGAG 
GAGGACGACG ACAAAGAAGT CGATCAGTAT GATGAACTGA AAATAGATAG CAAAGATAAC 
GAAATATTGA ACGAAGTGGC AGACGATAAA AAGCCAATTA ATGATAGTGA AACAACCGAA 
CTTAAGCCCA ATTTGGTTGA GCAAACGATA GATGGACCCA GTAGTGTAGA GAAACCAAAG 
GAAGAAGTAC CCAAAACTGA AGAGCAAATA GTGGAGCCAC AGGCGGCAGC ACCCGCAGCG 
GAGCAGGAGA ATAAGCAGCC GATTCCATCT ACCCATCAAA ACATTGTGCC TGCCACGGAA 
GCGCCAAAGA ATGGCACAAC TGAAGGTCAG CTGGAGGAGA TAGTTGCAAC AAAGCAGGTG 
CCTGAAACAA CGACTCCATT AGTAGAGGAG GTAACCGAAA AGGTAGAAGT GCCAACGATT 
GAAACGATTG AAGAAACCTC CAACGAAAAT GTCGAACAAC CAAAAGCAGT AGAGGTCGAA 
CCACCAAAAG CAGCAGAGAT CGAGGATACC ACAGAATCAC AGCCTCTGGT GTTACCCGAG 
GCCAGCGCCA CGGCCAGTCC CCCCCTACAG GAGGTTTCGC AAGTCGCAAC AGAGCCTTCA 
GTTAGCTTGC CGCCACTTTT CGATAAGCAA AACTTTGCTA ATCCAAACGA GTATTATAAA 
AAATTGCAAG AACAGCAGGA GGCAGTGCAT CGGCAACCGA GAGAAACATC CATTGATAAT 
CCAAATGATT ATTACAAACA ACAGCAAGAG CAGCAGCAAC AGCAGCAACA GCAACAGCAG 
CAGCAAGAGC AGCAGAAGAA GCAAGAGGAA CAACAGCAAC AGCAGGAGAA AGTTGAAGAG 
CAACTGAAAC AGCAACAGGA GAAAGTGGAG GAGAAGCAGC CACAGGAACA GCCTGAGCAA 
ACTACACCCA CACCCTCCTA CGAAGCACCC TATGCAGCAG TGGATGATGA GCCCACGGCA 
GCACCAACTA GCGAGGAGAT ATCCACGAAG AACGGTGGAG TTGGTTCGGT GGAACCATTG 
GCCCTGCCCT CGACAGCAAT TCCAGCGTCA GAGGCTTCCA TAAGTCCCAT AAAAGAGGAT 
GGCGTGGGTG GTGGTGGGGG TCTCTTTGCG ACCATTGTCG ACACTGTCAA CACATTCATC 
TCTAGTCAGG GATCCAATTC GAAGGAGAAT GTGCCATCGG AAAAGAGCAG CGATGAACTG 
CAACGAATTC TGTATCCGGG CATGGGTGAA AAGCCATCTA GAGATGTTCC CCCTGCCGAT 
GTCGATGGCC AGTGTGTCCG GTACCAGGCC AACGATAATG ATAATGAACA TTGCCATCGC 
ACGATTTCGC TGGACAACTT TGCAGAGGTG CTGGCGGGGA AGCTGGTGGA TCACAGCCAG 
CTGCTGTTGT GCGTGGTCAT TGCGGCCGCT TCTTCGCTGT TCTTCATCTT CGGGTACTAC 
TGCTTTTGCA ACAGCAGCAA AGAGGGAGCT CTGCTCTCAA AACTGAACCA CTTAGAGCGC 
AGCCTGCTGA CATCGCACAA GGAGAATCTC ATCATAAAGA ACGACCTGAT GACCACACGC 
ACCAAACTGG CCAGCATTGA GGACAACTCC TTTGGGTCAA ATGACATGGT GGTGGCACTC 
AAGAAGCAGC TGGAGATTGA GTTGTACGAG AAGGGAAAGC TACAGGAGCA GGTTGTCTCC 
CTGGAAAGGG ATGTGGACAA TGCCGCGGAG GCTGGTCTAG AGCTGAACAA AATGCTTTCA 
GAGGTGTTGG GCAACCAGAG TGGCGATGAT GCCTTCATGA GCACCGTTGA GGACTTGCAG 
CGACAGCTAA ACGATCAGGA AAAGATCATT GTTGACATCA ACTCGAGCCT CGCGGAGAAG 
AGCCGAGAGA ATAGTGAGCT GCAATATACT TACACCGAGT CCACGGCGCG TCTGACGAGC 
GAACTGAAGT CGCTGCAGGA GGATAACTAC GAGCTGGATA TCGAAAAGTC CAAGCTGAAG 
ACGCGGCTCG AGGAAATCCA AGACGAGAAC GAGCAGGAAT TGGCCAAGGC CCTGGAGGCT 
CGCAACTTTG AGATGCAGCG TCTGCAGAGC CAGATCCTCG ATCTAACCAC CAAGTGGGAG 
AAGGAGCACA GCGATCTGCA GACCAGTCTA GCCAAGATCG AGGCTCTCGA GGACTGCCTG 
AAGTCTGTGA AGAAGGATGG CAACCTGAAT GTCAACGAGC TAATAAGCTC GGCCAAGACG 
CGCGGTGAGC TGAACGCGGC CCAGAAGAAG TTTGCCGATC TGCAGGCGAA GCTGGAGCAG 
GAGCAGGTCC ACAAGGGACG ACTGGAGAGT CAGCTGAGGC AGTCCAATGT CGATGTGGAG 
CAGCTGAAGC AGGACTTCAA TCAGTCGGAG CGCGACAAGC TGGAGGCGCA GACGCGTCTC 
GAAGTGCTCT CTGGATACTT TCGGGAAAAG GAAAACGAAC TTAAAACGGA ACTCAGCTTG 
CAGGAGACCA AGTGGATGCA GCATCAGGGC GAGAATACCA GCACCGTGGA GACCCAGACG 
CTGATGAAGA ATGAAATTCA GACGCTCAAA TCTCAAAACG ATGAGCTGCG CGCGGAGATC 
GAGGCACAGA TTGCCTCACA CAAGGCCCAG ATGGGCACAC TGGAGAATCG GGCGCACGAG 
TCCTGGCTGG CGGCGCGTCA GTCGGAGCGA CGCTGTGAGG AGGCGCTAGC TGAGGCCGCC 
GGCCTGCGAC GCAAGCTAAC CACCATGGCC GTCAGTGGTG AGGCAGCGGC AGTAGACTCC 
GTCTCCTTCT CGAATGGAGG AGAATCGAAG GCAACCACAG CTCCCTCGCC GCTGCCGCTG 
CCTTTGCCGG GCTCGCCATT ACTAAACATG CCAAATCCAC TGCCATTCCT GGCGGCTCCC 
TTCTCACCAT TTATGGGCCT GCCGCCGCCA TTCCTGCCGC CAGCAGCCGC TGGTGGAGCA 
CGGCCACCGC CGCTTGGTCG CATGCGTTCC CCGCCACCGG GTATGTCTGC ATCCCGCGGA 
CACCGTGATC GGGATCGTGA ACGGTACTCG GACTACAGCG ACTACGATGA CTACGACGAC 
GATGAGGAGG ATGATCGCCG CCGCCGGCAT AGTGGCAGCT GGGGTCGGGG ACGACGCGAC 
TCCTACACGA ACTCGCCTCG CACATATCGC TCGCTCTCCC CCTCGGACAG TCGTTACAAT 
TACCGGCACG ACACAGAGAC GGACTACAGT CCGCCGCCCA GTCCGCGTCC GATGGCGGCG 
GGACACCGCA ACGGTCCGGG ACCGGGAGCG GGGGCTGGAT CGCGGACCTA CAGCGAAGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001355794.3
CDS	132..4454
Translation	MRLTNENLKLHPMLRNLAIFLSLTIFICLPTTWAAETATLSDKRLCADPKCEQIISLGTTKILYNSGGKGLISFKINTPVRILSKSAGTNKQLWGVDIGGRRGYANKDFILENRIMITEKDLKYEVPVVGPGSPPPAVAPAATPPEAPLKATEVEPAVLNASESADELATTTTSPLDVKEDSIVTKKVEVDDQQVPDVPTASSQVLQMVDGTQLPMEAIPAVTERTHDQQVVPPTQKSQTASAAVDTKVPQPVAPTIQSNQSQEQQQQLEKAVTDQNEPTPAVTEPQKLQQVEIEHKDPQEAAATEAASAPKKSEPEPVTVPPVAINADIDDDSDDFDYEEDDDKEVDQYDELKIDSKDNEILNEVADDKKPINDSETTELKPNLVEQTIDGPSSVEKPKEEVPKTEEQIVEPQAAAPAAEQENKQPIPSTHQNIVPATEAPKNGTTEGQLEEIVATKQVPETTTPLVEEVTEKVEVPTIETIEETSNENVEQPKAVEVEPPKAAEIEDTTESQPLVLPEASATASPPLQEVSQVATEPSVSLPPLFDKQNFANPNEYYKKLQEQQEAVHRQPRETSIDNPNDYYKQQQEQQQQQQQQQQQQEQQKKQEEQQQQQEKVEEQLKQQQEKVEEKQPQEQPEQTTPTPSYEAPYAAVDDEPTAAPTSEEISTKNGGVGSVEPLALPSTAIPASEASISPIKEDGVGGGGGLFATIVDTVNTFISSQGSNSKENVPSEKSSDELQRILYPGMGEKPSRDVPPADVDGQCVRYQANDNDNEHCHRTISLDNFAEVLAGKLVDHSQLLLCVVIAAASSLFFIFGYYCFCNSSKEGALLSKLNHLERSLLTSHKENLIIKNDLMTTRTKLASIEDNSFGSNDMVVALKKQLEIELYEKGKLQEQVVSLERDVDNAAEAGLELNKMLSEVLGNQSGDDAFMSTVEDLQRQLNDQEKIIVDINSSLAEKSRENSELQYTYTESTARLTSELKSLQEDNYELDIEKSKLKTRLEEIQDENEQELAKALEARNFEMQRLQSQILDLTTKWEKEHSDLQTSLAKIEALEDCLKSVKKDGNLNVNELISSAKTRGELNAAQKKFADLQAKLEQEQVHKGRLESQLRQSNVDVEQLKQDFNQSERDKLEAQTRLEVLSGYFREKENELKTELSLQETKWMQHQGENTSTVETQTLMKNEIQTLKSQNDELRAEIEAQIASHKAQMGTLENRAHESWLAARQSERRCEEALAEAAGLRRKLTTMAVSGEAAAVDSVSFSNGGESKATTAPSPLPLPLPGSPLLNMPNPLPFLAAPFSPFMGLPPPFLPPAAAGGARPPPLGRMRSPPPGMSASRGHRDRDRERYSDYSDYDDYDDDEEDDRRRRHSGSWGRGRRDSYTNSPRTYRSLSPSDSRYNYRHDTETDYSPPPSPRPMAAGHRNGPGPGAGAGSRTYSEV

Target ORF information:

RefSeq Version	XM_001355758.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10757), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001355758.3

ORF Insert Sequence:

ATGCGGCTGA CAAACGAGAA CTTGAAACTC CACCCAATGC TGAGGAATTT GGCCATTTTC 
CTCAGCCTAA CAATCTTCAT TTGTCTTCCA ACGACGTGGG CCGCAGAGAC GGCGACTCTC 
TCGGACAAAA GACTGTGCGC TGACCCAAAA TGTGAACAAA TCATCTCCTT GGGCACCACC 
AAGATACTGT ACAACTCTGG CGGCAAGGGA CTGATCTCCT TCAAAATCAA CACCCCAGTG 
CGCATCTTGT CCAAAAGTGC TGGCACGAAT AAGCAGCTCT GGGGCGTGGA TATCGGCGGA 
CGTCGTGGCT ATGCCAACAA GGATTTCATA TTGGAGAACA GGATCATGAT AACCGAGAAG 
GATTTAAAGT ATGAAGTGCC AGTGGTTGGA CCTGGTAGTC CACCACCCGC CGTGGCACCT 
GCTGCCACAC CACCAGAAGC TCCGCTGAAG GCGACAGAGG TAGAGCCGGC TGTACTTAAT 
GCCTCTGAGT CAGCCGATGA GTTGGCAACG ACTACAACCA GCCCGCTTGA TGTAAAAGAG 
GATTCCATTG TCACGAAAAA GGTTGAGGTG GATGACCAAC AGGTACCCGA TGTTCCAACC 
GCGTCATCTC AAGTCCTGCA GATGGTAGAC GGCACTCAGC TGCCAATGGA GGCAATTCCA 
GCTGTCACAG AGAGAACTCA CGATCAACAG GTCGTCCCAC CCACACAGAA ATCTCAAACA 
GCCTCAGCAG CGGTTGACAC GAAAGTGCCA CAACCGGTGG CCCCAACGAT TCAATCGAAT 
CAGTCACAGG AGCAGCAGCA GCAACTAGAG AAAGCAGTCA CTGACCAAAA TGAGCCGACG 
CCAGCAGTGA CGGAGCCTCA AAAGCTACAA CAGGTGGAAA TTGAACACAA AGACCCACAG 
GAGGCGGCAG CAACTGAAGC AGCGTCTGCT CCCAAGAAGT CGGAGCCAGA GCCAGTGACA 
GTGCCACCGG TGGCCATCAA CGCTGATATT GACGACGACT CTGATGATTT CGATTACGAG 
GAGGACGACG ACAAAGAAGT CGATCAGTAT GATGAACTGA AAATAGATAG CAAAGATAAC 
GAAATATTGA ACGAAGTGGC AGACGATAAA AAGCCAATTA ATGATAGTGA AACAACCGAA 
CTTAAGCCCA ATTTGGTTGA GCAAACGATA GATGGACCCA GTAGTGTAGA GAAACCAAAG 
GAAGAAGTAC CCAAAACTGA AGAGCAAATA GTGGAGCCAC AGGCGGCAGC ACCCGCAGCG 
GAGCAGGAGA ATAAGCAGCC GATTCCATCT ACCCATCAAA ACATTGTGCC TGCCACGGAA 
GCGCCAAAGA ATGGCACAAC TGAAGGTCAG CTGGAGGAGA TAGTTGCAAC AAAGCAGGTG 
CCTGAAACAA CGACTCCATT AGTAGAGGAG GTAACCGAAA AGGTAGAAGT GCCAACGATT 
GAAACGATTG AAGAAACCTC CAACGAAAAT GTCGAACAAC CAAAAGCAGT AGAGGTCGAA 
CCACCAAAAG CAGCAGAGAT CGAGGATACC ACAGAATCAC AGCCTCTGGT GTTACCCGAG 
GCCAGCGCCA CGGCCAGTCC CCCCCTACAG GAGGTTTCGC AAGTCGCAAC AGAGCCTTCA 
GTTAGCTTGC CGCCACTTTT CGATAAGCAA AACTTTGCTA ATCCAAACGA GTATTATAAA 
AAATTGCAAG AACAGCAGGA GGCAGTGCAT CGGCAACCGA GAGAAACATC CATTGATAAT 
CCAAATGATT ATTACAAACA ACAGCAAGAG CAGCAGCAAC AGCAGCAACA GCAACAGCAG 
CAGCAAGAGC AGCAGAAGAA GCAAGAGGAA CAACAGCAAC AGCAGGAGAA AGTTGAAGAG 
CAACTGAAAC AGCAACAGGA GAAAGTGGAG GAGAAGCAGC CACAGGAACA GCCTGAGCAA 
ACTACACCCA CACCCTCCTA CGAAGCACCC TATGCAGCAG TGGATGATGA GCCCACGGCA 
GCACCAACTA GCGAGGAGAT ATCCACGAAG AACGGTGGAG TTGGTTCGGT GGAACCATTG 
GCCCTGCCCT CGACAGCAAT TCCAGCGTCA GAGGCTTCCA TAAGTCCCAT AAAAGAGGAT 
GGCGTGGGTG GTGGTGGGGG TCTCTTTGCG ACCATTGTCG ACACTGTCAA CACATTCATC 
TCTAGTCAGG GATCCAATTC GAAGGAGAAT GTGCCATCGG AAAAGAGCAG CGATGAACTG 
CAACGAATTC TGTATCCGGG CATGGGTGAA AAGCCATCTA GAGATGTTCC CCCTGCCGAT 
GTCGATGGCC AGTGTGTCCG GTACCAGGCC AACGATAATG ATAATGAACA TTGCCATCGC 
ACGATTTCGC TGGACAACTT TGCAGAGGTG CTGGCGGGGA AGCTGGTGGA TCACAGCCAG 
CTGCTGTTGT GCGTGGTCAT TGCGGCCGCT TCTTCGCTGT TCTTCATCTT CGGGTACTAC 
TGCTTTTGCA ACAGCAGCAA AGAGGGAGCT CTGCTCTCAA AACTGAACCA CTTAGAGCGC 
AGCCTGCTGA CATCGCACAA GGAGAATCTC ATCATAAAGA ACGACCTGAT GACCACACGC 
ACCAAACTGG CCAGCATTGA GGACAACTCC TTTGGGTCAA ATGACATGGT GGTGGCACTC 
AAGAAGCAGC TGGAGATTGA GTTGTACGAG AAGGGAAAGC TACAGGAGCA GGTTGTCTCC 
CTGGAAAGGG ATGTGGACAA TGCCGCGGAG GCTGGTCTAG AGCTGAACAA AATGCTTTCA 
GAGGTGTTGG GCAACCAGAG TGGCGATGAT GCCTTCATGA GCACCGTTGA GGACTTGCAG 
CGACAGCTAA ACGATCAGGA AAAGATCATT GTTGACATCA ACTCGAGCCT CGCGGAGAAG 
AGCCGAGAGA ATAGTGAGCT GCAATATACT TACACCGAGT CCACGGCGCG TCTGACGAGC 
GAACTGAAGT CGCTGCAGGA GGATAACTAC GAGCTGGATA TCGAAAAGTC CAAGCTGAAG 
ACGCGGCTCG AGGAAATCCA AGACGAGAAC GAGCAGGAAT TGGCCAAGGC CCTGGAGGCT 
CGCAACTTTG AGATGCAGCG TCTGCAGAGC CAGATCCTCG ATCTAACCAC CAAGTGGGAG 
AAGGAGCACA GCGATCTGCA GACCAGTCTA GCCAAGATCG AGGCTCTCGA GGACTGCCTG 
AAGTCTGTGA AGAAGGATGG CAACCTGAAT GTCAACGAGC TAATAAGCTC GGCCAAGACG 
CGCGGTGAGC TGAACGCGGC CCAGAAGAAG TTTGCCGATC TGCAGGCGAA GCTGGAGCAG 
GAGCAGGTCC ACAAGGGACG ACTGGAGAGT CAGCTGAGGC AGTCCAATGT CGATGTGGAG 
CAGCTGAAGC AGGACTTCAA TCAGTCGGAG CGCGACAAGC TGGAGGCGCA GACGCGTCTC 
GAAGTGCTCT CTGGATACTT TCGGGAAAAG GAAAACGAAC TTAAAACGGA ACTCAGCTTG 
CAGGAGACCA AGTGGATGCA GCATCAGGGC GAGAATACCA GCACCGTGGA GACCCAGACG 
CTGATGAAGA ATGAAATTCA GACGCTCAAA TCTCAAAACG ATGAGCTGCG CGCGGAGATC 
GAGGCACAGA TTGCCTCACA CAAGGCCCAG ATGGGCACAC TGGAGAATCG GGCGCACGAG 
TCCTGGCTGG CGGCGCGTCA GTCGGAGCGA CGCTGTGAGG AGGCGCTAGC TGAGGCCGCC 
GGCCTGCGAC GCAAGCTAAC CACCATGGCC GTCAGTGGTG AGGCAGCGGC AGTAGACTCC 
GTCTCCTTCT CGAATGGAGG AGAATCGAAG GCAACCACAG CTCCCTCGCC GCTGCCGCTG 
CCTTTGCCGG GCTCGCCATT ACTAAACATG CCAAATCCAC TGCCATTCCT GGCGGCTCCC 
TTCTCACCAT TTATGGGCCT GCCGCCGCCA TTCCTGCCGC CAGCAGCCGC TGGTGGAGCA 
CGGCCACCGC CGCTTGGTCG CATGCGTTCC CCGCCACCGG GTATGTCTGC ATCCCGCGGA 
CACCGTGATC GGGATCGTGA ACGGTACTCG GACTACAGCG ACTACGATGA CTACGACGAC 
GATGAGGAGG ATGATCGCCG CCGCCGGCAT AGTGGCAGCT GGGGTCGGGG ACGACGCGAC 
TCCTACACGA ACTCGCCTCG CACATATCGC TCGCTCTCCC CCTCGGACAG TCGTTACAAT 
TACCGGCACG ACACAGAGAC GGACTACAGT CCGCCGCCCA GTCCGCGTCC GATGGCGGCG 
GGACACCGCA ACGGTCCGGG ACCGGGAGCG GGGGCTGGAT CGCGGACCTA CAGCGAAGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA10757 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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