LOC6588851 cDNA ORF clone, Drosophila persimilis

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6588851
Entrez Gene ID	6588851
Full Name	GL19425 gene product from transcript GL19425-RA
Synonyms	GL19425,dper_GLEANR_21561
Gene Type	protein-coding
Organism	Drosophila persimilis

mRNA and Protein(s)

mRNA	Protein	Name
XM_002014870.1	XP_002014906.1	Dper\GL19425-PA
XM_026988482.1	XP_026844283.1	pre-mRNA-splicing factor CWC22 homolog isoform X1
XM_002014870.2	XP_002014906.1	pre-mRNA-splicing factor CWC22 homolog isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6588851 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6588851 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODb15370	XM_002014870.2 Latest version!	Drosophila persimilis pre-mRNA-splicing factor CWC22 homolog (LOC6588851), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODb22428	XM_026988482.1 Latest version!	Drosophila persimilis pre-mRNA-splicing factor CWC22 homolog (LOC6588851), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
ODb15370	XM_002014870.1	Drosophila persimilis GL19425 (Dper\GL19425), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6588851 ( XM_002014870.1 ) cDNA ORF clone, Drosophila persimilis -> XP_002014906.1 Drosophila persimilis GL19425 (Dper\GL19425), mRNA.

Price & Availability↑

CloneID	ODb15370
Clone ID Related Accession (Same CDS sequence)	XM_002014870.1 , XM_002014870.2
Accession Version	XM_002014870.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1322928000000
Organism	Drosophila persimilis
Product	Dper\GL19425-PA
Comment	Comment: PROVISIONAL REFSEQ: This record is based on preliminary annotation provided by FlyBase. This record is derived from an annotated genomic sequence (NW_001985954). COMPLETENESS: incomplete on both ends.

ATGGGGGAAA GCGATGCAGA AAGTGATTCG TGCAGTGGCT CCAGTAGTAG CAGTGGCTCC 
TCGAGCAGTT CCTCCACCAG TGGCAGCGGC AGTGCCTCCA AAAGTGATGC TGGTAGCCGT 
CTGGCAAGCG GTCAGGAGGA AAACGAAGAG GAAGAGCGCG GCCAGAAGGC AAAAGAAGTG 
GCAGAAGAAG AAGTGACGCC GGCAGAAGTA TCTAATGATT CCCCCGCCGT AGAGGAGCCG 
CCGCAAAAGA AGGAGGAGGA AGAAAAGAAA GTGCTACAAA CAGACGAAAA AGTTGCCTCT 
CCAAAAGAGG AGGACGAGCA AAAGCAAACC GCAGCAGCAA AGCCGGAAAA ACAATCAAAT 
GTTGACTCTT CCACAGCTGC TGTTGCTGTT GACACGTCTG TGTCGGCTGG CGTGGAAAAA 
GCAGAAGCAG AAGCAGTACC GACAGCAGCG GCGCCTGCAG AGGCAGCAGA AAAAGTAGAA 
GAGGAAGCTA TAACAAAATC AATTGAAGCG GAAACAGAAA CCGAAACATC TACCCCGCAA 
CGACCCTTGG ATGCCACAAC TGTGACTAAT TCCGATGAGA AACCGCAGCC AGAGGCAGCG 
GCAGTGGCAG TAACAGAGGA GGAACCTGAT AAAGATCATT CCGTGACCAC TAATGGAGCG 
GACAATGCAG CAGCAGCACC ACCACCAACA GCTGCCAGTC CATCGAATCA CATCGAAGAG 
GGCGAAATAA CTGATAAGGA CGACGACGAC GATAATGTGC CGCCGCCTGT GCCTGTGCCT 
GTGCCCAAGC AGACGCTGGC CAGCGAAGTG CGCAAACGTA GTCGCAGTGG TGATGAAAAG 
AGCCGTAACC GGCGACGCAG TCCCAGCCGC AGTCGCCGTC GCACCCGTAC ACGCAGTCGC 
AGCAAGAGCC CGAAGCGTCG CCGCTCCAGC TCCTTGGAGC GGAGACGCCG CGAACGTCAG 
AAGCGGCACG AGGAGCGCGA TAAACGCGAT CAGGTGCGGG CGAGGGAGCG CGAAGATCGT 
CGTCGCAATG AAAAGTCGCG CAGCAGTCGG CGCGATGATT CTCGCGAGAG ATCCAGGCGG 
AGGTCCAGCG ACAAGCGGCG CTCCAGCTCG AGTGGCGGCT CCAAGAGGGC AGGTTCGGCA 
ATCGCCGACA ATGCCACGGT CACAAATCCC ACGGCCAAGA TCACAGAACG CCAGCGTCGC 
ACTGTGGATG TGCTGACATC GCGCACCGGA GGCGCTTACA TACCGCCAGC GAAGCTCCGG 
CTGCTGCAGG AGGGGATCAC GGACAAGGCA TCGGCTGCGT ATCAGCGCAT CGCCTGGGAG 
GCGCTCAAGA AGTCCATTCA CGGGTACATC AACAAGGTGA ATGTCACAAA TATTGCCATC 
ATTACCCGCG AACTCCTGCG GGAGAACATC GTCCGTGGAC GGGGCCTGCT GTCGAGGAGC 
ATCATCCAGG CCCAGGCCGC CTCGCCCACC TTCACGCACG TGTATGCCGC TCTGGTGGCC 
ATTATCAATT CGAAATTCCC GAATATCGGC GAGCTGCTGC TCAAGCGTCT GGTCATTCAG 
TTCCGCCGTG CCTTTCGGCG CAATGACAAG CTCGTCTGCT TGTCGTCCAC TCGATTCATT 
GCTCATCTGG TGAATCAGCG TGTGGCCCAC GAAATACTGG CCCTGGAGAT TCTCACGCTC 
CTGGTGGAGA CGCCCACCGA TGATTCCGTG GAGGTGGCCA TTGCCTTTCT CAAGGAGTGC 
GGCATGAAAC TGACGGAGGT CTCGTCCAAG GGCATTGGGG CCATTTTTGA GATGCTGCGC 
AACATCCTGC ACGAGGGAAA GCTGGACAAA CGAGTACAGT ACATGATCGA GGTGCTGTTT 
CAAGTGCGCA AGGATGGCTT CAAGGATCAT CTGGCCATTG TGGGAGAACT GGAGCTGGTC 
GAAGAGGACG AGCAGTTTAC GCATCTCATG ATGCTGGACG AGGCCACCGA AACGGAGGAT 
ATACTGAATG TCTTCAAATT TGACGAGAAC TTTGCCGAAA ACGAGGACAA ATACAAGGGT 
CTGAGTGCCG AAATCCTGGG CAGCGATGAT GGCAGCGGCT CGGGCTCTGG CTCTGGCTCG 
GGCTCCGATT CCGACAGCGA TTCGGATGGT GAAATCAACT CGGATGCCGA AGTCGGCAAA 
AAGACCGATG CCGGGGATAT TATCGACAAC ACGGAGACGA ATCTAATTGC TCTGCGGCGC 
ACCATATATC TGACGATCAA CTCGAGTTTG GATTACGAGG AGTGCGCCCA CAAGCTGATG 
AAAATGGAGC TGAAGCCGGG GCAGGAGGTG GAGCTGTGCC ACATGTTCCT CGACTGCTGT 
GCCGAGCAGC GGACCTACGA GAAGTTCTAC GGCCTGTTGG CGCAGCGTTT CTGCAACATC 
AATCGAATGT ATATACCCCC CTTCGAGGAG ATCTTCAAGG ACACCTACCA GACGACGCAT 
CGCCTGGACA CGAATCGGTT GCGGAATGTG AGCAAGTTCT TTGCCCATCT GCTCTTCACC 
GATTCCATCA GCTGGGATGT CTTGGACTGT GTCCTGCTGA ACGAGGACGA TACCACCTCA 
TCCAGTCGCA TTTTCATCAA GATTCTGTTC CAGGAGCTCG CCGAATACAT GGGCTTGGGC 
AAACTCAACT CCAAGCTCAA GGAAGAGGTG CTGGTGGAGA GCCTGGCGGG TCTCTTCCCC 
AAGGATAATC CTCGGAACAC ACGATTCTCC ATCAATTTCT TCACGTCCAT TGGTTTGGGT 
GGACTCACCG ATGATCTGCG ACGTTTCCTA AAGACAGCGC CCAAAGCAGT GCCCGCGATC 
AATTCAGAGC TCCTGTCCAC TGGGGGGAAT CCATTCCGGG ATCCAGGATC TGCCCCAGCA 
GCGGGGGCAG TACCCATTGC TGTGACTTCC TCCTCTGCCG GTTCGTCGAG CAGTTCCAGT 
AGCAGCAGCA GCGGAGACTC CAGCGAGCAA AAGTCGAGCA CCAGCGATGA GGACAGCGAC 
AGTGAATCCT CCAGCTCCGA GTCCGAGCCA CAGCGAAAGC GTAAACGCAA GGAGAAGCGC 
AAGAAGAAGC CCAAAAAGCA GCGAAAATCA GAGCTCAAAA AGTTAAAGGA CAAAAAAGAC 
AAAAAGAAGA AATCCAAAAA GGAAAAGGAT AAAGAGAAGG AGCGGGGGCG CGAGGAGAAA 
AAGAAGCGCG AAAAGGAGAA GAAAAAGGCC GCCAAAAAGA AGTCGAAACG CAAGCGCAAG 
CACCAGGAGA GCTCCAGTTC CAGCAGCAGC TCTGAAAGCA GCAAGGCCTC CTCCACATCC 
TCCTCAGCAT CAGAGGCCGC CTCGAGCAGC GACGACGACG ACAGCGATGG CCAGCCCCAG 
CCGAAGATCA TGCGCCAGGA GCAGAGCAGC AAAATCCACA ACAACCACAA CAGCAACAAC 
AAATTCAAGA CCCGAAAGGA AGACAGCGAT GGATTCAACC TGGAGGGACC ACCCATGGGC 
CGAGAACAGG AGCGCCAACC ACATTATCAT CATCAGCTCA ATGGAAATGG TGCCAGGAAG 
CGGGAGCCAT CGTTTTCTGC CCATGAGGAA AGACACCCGG AGCGGCGAGG GGAGCGAGAT 
GCCCGTCGTG GAGAATCCCG AGGTCTTCGT GGAGACTCCG GTGGCCGACG TGGAGACTCC 
GGTAGTCGTC GTGGAGAATC GCGAGGTCTT CGTGGAGACT CCGGTGGCCG TCGTCCAGAA 
ACCCGACGAG GCGAATCCCA AGGCCGCCGA AACGATAGAG GAGAACAACG TGGTGGCCGC 
CTCGGCAGTG ATCGTGATCG AGAACGTGAC AGAGGAGAAC GAGATCAGGA AAGAAATAGA 
GAACGTGAGC GGGAGCGGGA ACGGGATCAT GACCGTCGTG ACAATCGAGA CCGTGACCGT 
GATCGGGATC GTGATCACCC ACGTAATCGA GAACGTTCCC GTGACCAGGA TCGCGCCAGA 
AAACCAGAAC GAGAACGCAG CTCTCACAAG CGCGTGTCCA AGGGGCGCGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002014906.1
CDS	1..4014
Translation	MGESDAESDSCSGSSSSSGSSSSSSTSGSGSASKSDAGSRLASGQEENEEEERGQKAKEVAEEEVTPAEVSNDSPAVEEPPQKKEEEEKKVLQTDEKVASPKEEDEQKQTAAAKPEKQSNVDSSTAAVAVDTSVSAGVEKAEAEAVPTAAAPAEAAEKVEEEAITKSIEAETETETSTPQRPLDATTVTNSDEKPQPEAAAVAVTEEEPDKDHSVTTNGADNAAAAPPPTAASPSNHIEEGEITDKDDDDDNVPPPVPVPVPKQTLASEVRKRSRSGDEKSRNRRRSPSRSRRRTRTRSRSKSPKRRRSSSLERRRRERQKRHEERDKRDQVRAREREDRRRNEKSRSSRRDDSRERSRRRSSDKRRSSSSGGSKRAGSAIADNATVTNPTAKITERQRRTVDVLTSRTGGAYIPPAKLRLLQEGITDKASAAYQRIAWEALKKSIHGYINKVNVTNIAIITRELLRENIVRGRGLLSRSIIQAQAASPTFTHVYAALVAIINSKFPNIGELLLKRLVIQFRRAFRRNDKLVCLSSTRFIAHLVNQRVAHEILALEILTLLVETPTDDSVEVAIAFLKECGMKLTEVSSKGIGAIFEMLRNILHEGKLDKRVQYMIEVLFQVRKDGFKDHLAIVGELELVEEDEQFTHLMMLDEATETEDILNVFKFDENFAENEDKYKGLSAEILGSDDGSGSGSGSGSGSDSDSDSDGEINSDAEVGKKTDAGDIIDNTETNLIALRRTIYLTINSSLDYEECAHKLMKMELKPGQEVELCHMFLDCCAEQRTYEKFYGLLAQRFCNINRMYIPPFEEIFKDTYQTTHRLDTNRLRNVSKFFAHLLFTDSISWDVLDCVLLNEDDTTSSSRIFIKILFQELAEYMGLGKLNSKLKEEVLVESLAGLFPKDNPRNTRFSINFFTSIGLGGLTDDLRRFLKTAPKAVPAINSELLSTGGNPFRDPGSAPAAGAVPIAVTSSSAGSSSSSSSSSSGDSSEQKSSTSDEDSDSESSSSESEPQRKRKRKEKRKKKPKKQRKSELKKLKDKKDKKKKSKKEKDKEKERGREEKKKREKEKKKAAKKKSKRKRKHQESSSSSSSSESSKASSTSSSASEAASSSDDDDSDGQPQPKIMRQEQSSKIHNNHNSNNKFKTRKEDSDGFNLEGPPMGREQERQPHYHHQLNGNGARKREPSFSAHEERHPERRGERDARRGESRGLRGDSGGRRGDSGSRRGESRGLRGDSGGRRPETRRGESQGRRNDRGEQRGGRLGSDRDRERDRGERDQERNRERERERERDHDRRDNRDRDRDRDRDHPRNRERSRDQDRARKPERERSSHKRVSKGRG

Target ORF information:

RefSeq Version	XM_002014870.1
Organism	Drosophila persimilis
Definition	Drosophila persimilis GL19425 (Dper\GL19425), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002014870.1

ORF Insert Sequence:

ATGGGGGAAA GCGATGCAGA AAGTGATTCG TGCAGTGGCT CCAGTAGTAG CAGTGGCTCC 
TCGAGCAGTT CCTCCACCAG TGGCAGCGGC AGTGCCTCCA AAAGTGATGC TGGTAGCCGT 
CTGGCAAGCG GTCAGGAGGA AAACGAAGAG GAAGAGCGCG GCCAGAAGGC AAAAGAAGTG 
GCAGAAGAAG AAGTGACGCC GGCAGAAGTA TCTAATGATT CCCCCGCCGT AGAGGAGCCG 
CCGCAAAAGA AGGAGGAGGA AGAAAAGAAA GTGCTACAAA CAGACGAAAA AGTTGCCTCT 
CCAAAAGAGG AGGACGAGCA AAAGCAAACC GCAGCAGCAA AGCCGGAAAA ACAATCAAAT 
GTTGACTCTT CCACAGCTGC TGTTGCTGTT GACACGTCTG TGTCGGCTGG CGTGGAAAAA 
GCAGAAGCAG AAGCAGTACC GACAGCAGCG GCGCCTGCAG AGGCAGCAGA AAAAGTAGAA 
GAGGAAGCTA TAACAAAATC AATTGAAGCG GAAACAGAAA CCGAAACATC TACCCCGCAA 
CGACCCTTGG ATGCCACAAC TGTGACTAAT TCCGATGAGA AACCGCAGCC AGAGGCAGCG 
GCAGTGGCAG TAACAGAGGA GGAACCTGAT AAAGATCATT CCGTGACCAC TAATGGAGCG 
GACAATGCAG CAGCAGCACC ACCACCAACA GCTGCCAGTC CATCGAATCA CATCGAAGAG 
GGCGAAATAA CTGATAAGGA CGACGACGAC GATAATGTGC CGCCGCCTGT GCCTGTGCCT 
GTGCCCAAGC AGACGCTGGC CAGCGAAGTG CGCAAACGTA GTCGCAGTGG TGATGAAAAG 
AGCCGTAACC GGCGACGCAG TCCCAGCCGC AGTCGCCGTC GCACCCGTAC ACGCAGTCGC 
AGCAAGAGCC CGAAGCGTCG CCGCTCCAGC TCCTTGGAGC GGAGACGCCG CGAACGTCAG 
AAGCGGCACG AGGAGCGCGA TAAACGCGAT CAGGTGCGGG CGAGGGAGCG CGAAGATCGT 
CGTCGCAATG AAAAGTCGCG CAGCAGTCGG CGCGATGATT CTCGCGAGAG ATCCAGGCGG 
AGGTCCAGCG ACAAGCGGCG CTCCAGCTCG AGTGGCGGCT CCAAGAGGGC AGGTTCGGCA 
ATCGCCGACA ATGCCACGGT CACAAATCCC ACGGCCAAGA TCACAGAACG CCAGCGTCGC 
ACTGTGGATG TGCTGACATC GCGCACCGGA GGCGCTTACA TACCGCCAGC GAAGCTCCGG 
CTGCTGCAGG AGGGGATCAC GGACAAGGCA TCGGCTGCGT ATCAGCGCAT CGCCTGGGAG 
GCGCTCAAGA AGTCCATTCA CGGGTACATC AACAAGGTGA ATGTCACAAA TATTGCCATC 
ATTACCCGCG AACTCCTGCG GGAGAACATC GTCCGTGGAC GGGGCCTGCT GTCGAGGAGC 
ATCATCCAGG CCCAGGCCGC CTCGCCCACC TTCACGCACG TGTATGCCGC TCTGGTGGCC 
ATTATCAATT CGAAATTCCC GAATATCGGC GAGCTGCTGC TCAAGCGTCT GGTCATTCAG 
TTCCGCCGTG CCTTTCGGCG CAATGACAAG CTCGTCTGCT TGTCGTCCAC TCGATTCATT 
GCTCATCTGG TGAATCAGCG TGTGGCCCAC GAAATACTGG CCCTGGAGAT TCTCACGCTC 
CTGGTGGAGA CGCCCACCGA TGATTCCGTG GAGGTGGCCA TTGCCTTTCT CAAGGAGTGC 
GGCATGAAAC TGACGGAGGT CTCGTCCAAG GGCATTGGGG CCATTTTTGA GATGCTGCGC 
AACATCCTGC ACGAGGGAAA GCTGGACAAA CGAGTACAGT ACATGATCGA GGTGCTGTTT 
CAAGTGCGCA AGGATGGCTT CAAGGATCAT CTGGCCATTG TGGGAGAACT GGAGCTGGTC 
GAAGAGGACG AGCAGTTTAC GCATCTCATG ATGCTGGACG AGGCCACCGA AACGGAGGAT 
ATACTGAATG TCTTCAAATT TGACGAGAAC TTTGCCGAAA ACGAGGACAA ATACAAGGGT 
CTGAGTGCCG AAATCCTGGG CAGCGATGAT GGCAGCGGCT CGGGCTCTGG CTCTGGCTCG 
GGCTCCGATT CCGACAGCGA TTCGGATGGT GAAATCAACT CGGATGCCGA AGTCGGCAAA 
AAGACCGATG CCGGGGATAT TATCGACAAC ACGGAGACGA ATCTAATTGC TCTGCGGCGC 
ACCATATATC TGACGATCAA CTCGAGTTTG GATTACGAGG AGTGCGCCCA CAAGCTGATG 
AAAATGGAGC TGAAGCCGGG GCAGGAGGTG GAGCTGTGCC ACATGTTCCT CGACTGCTGT 
GCCGAGCAGC GGACCTACGA GAAGTTCTAC GGCCTGTTGG CGCAGCGTTT CTGCAACATC 
AATCGAATGT ATATACCCCC CTTCGAGGAG ATCTTCAAGG ACACCTACCA GACGACGCAT 
CGCCTGGACA CGAATCGGTT GCGGAATGTG AGCAAGTTCT TTGCCCATCT GCTCTTCACC 
GATTCCATCA GCTGGGATGT CTTGGACTGT GTCCTGCTGA ACGAGGACGA TACCACCTCA 
TCCAGTCGCA TTTTCATCAA GATTCTGTTC CAGGAGCTCG CCGAATACAT GGGCTTGGGC 
AAACTCAACT CCAAGCTCAA GGAAGAGGTG CTGGTGGAGA GCCTGGCGGG TCTCTTCCCC 
AAGGATAATC CTCGGAACAC ACGATTCTCC ATCAATTTCT TCACGTCCAT TGGTTTGGGT 
GGACTCACCG ATGATCTGCG ACGTTTCCTA AAGACAGCGC CCAAAGCAGT GCCCGCGATC 
AATTCAGAGC TCCTGTCCAC TGGGGGGAAT CCATTCCGGG ATCCAGGATC TGCCCCAGCA 
GCGGGGGCAG TACCCATTGC TGTGACTTCC TCCTCTGCCG GTTCGTCGAG CAGTTCCAGT 
AGCAGCAGCA GCGGAGACTC CAGCGAGCAA AAGTCGAGCA CCAGCGATGA GGACAGCGAC 
AGTGAATCCT CCAGCTCCGA GTCCGAGCCA CAGCGAAAGC GTAAACGCAA GGAGAAGCGC 
AAGAAGAAGC CCAAAAAGCA GCGAAAATCA GAGCTCAAAA AGTTAAAGGA CAAAAAAGAC 
AAAAAGAAGA AATCCAAAAA GGAAAAGGAT AAAGAGAAGG AGCGGGGGCG CGAGGAGAAA 
AAGAAGCGCG AAAAGGAGAA GAAAAAGGCC GCCAAAAAGA AGTCGAAACG CAAGCGCAAG 
CACCAGGAGA GCTCCAGTTC CAGCAGCAGC TCTGAAAGCA GCAAGGCCTC CTCCACATCC 
TCCTCAGCAT CAGAGGCCGC CTCGAGCAGC GACGACGACG ACAGCGATGG CCAGCCCCAG 
CCGAAGATCA TGCGCCAGGA GCAGAGCAGC AAAATCCACA ACAACCACAA CAGCAACAAC 
AAATTCAAGA CCCGAAAGGA AGACAGCGAT GGATTCAACC TGGAGGGACC ACCCATGGGC 
CGAGAACAGG AGCGCCAACC ACATTATCAT CATCAGCTCA ATGGAAATGG TGCCAGGAAG 
CGGGAGCCAT CGTTTTCTGC CCATGAGGAA AGACACCCGG AGCGGCGAGG GGAGCGAGAT 
GCCCGTCGTG GAGAATCCCG AGGTCTTCGT GGAGACTCCG GTGGCCGACG TGGAGACTCC 
GGTAGTCGTC GTGGAGAATC GCGAGGTCTT CGTGGAGACT CCGGTGGCCG TCGTCCAGAA 
ACCCGACGAG GCGAATCCCA AGGCCGCCGA AACGATAGAG GAGAACAACG TGGTGGCCGC 
CTCGGCAGTG ATCGTGATCG AGAACGTGAC AGAGGAGAAC GAGATCAGGA AAGAAATAGA 
GAACGTGAGC GGGAGCGGGA ACGGGATCAT GACCGTCGTG ACAATCGAGA CCGTGACCGT 
GATCGGGATC GTGATCACCC ACGTAATCGA GAACGTTCCC GTGACCAGGA TCGCGCCAGA 
AAACCAGAAC GAGAACGCAG CTCTCACAAG CGCGTGTCCA AGGGGCGCGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6588851 ( XM_002014870.2 ) cDNA ORF clone, Drosophila persimilis -> XP_002014906.1 Drosophila persimilis pre-mRNA-splicing factor CWC22 homolog (LOC6588851), transcript variant X2, mRNA.

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CloneID	ODb15370
Clone ID Related Accession (Same CDS sequence)	XM_002014870.1 , XM_002014870.2
Accession Version	XM_002014870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541433600000
Organism	Drosophila persimilis
Product	pre-mRNA-splicing factor CWC22 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825298.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 7, 2018 this sequence version replaced XM_002014870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila persimilis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAA GCGATGCAGA AAGTGATTCG TGCAGTGGCT CCAGTAGTAG CAGTGGCTCC 
TCGAGCAGTT CCTCCACCAG TGGCAGCGGC AGTGCCTCCA AAAGTGATGC TGGTAGCCGT 
CTGGCAAGCG GTCAGGAGGA AAACGAAGAG GAAGAGCGCG GCCAGAAGGC AAAAGAAGTG 
GCAGAAGAAG AAGTGACGCC GGCAGAAGTA TCTAATGATT CCCCCGCCGT AGAGGAGCCG 
CCGCAAAAGA AGGAGGAGGA AGAAAAGAAA GTGCTACAAA CAGACGAAAA AGTTGCCTCT 
CCAAAAGAGG AGGACGAGCA AAAGCAAACC GCAGCAGCAA AGCCGGAAAA ACAATCAAAT 
GTTGACTCTT CCACAGCTGC TGTTGCTGTT GACACGTCTG TGTCGGCTGG CGTGGAAAAA 
GCAGAAGCAG AAGCAGTACC GACAGCAGCG GCGCCTGCAG AGGCAGCAGA AAAAGTAGAA 
GAGGAAGCTA TAACAAAATC AATTGAAGCG GAAACAGAAA CCGAAACATC TACCCCGCAA 
CGACCCTTGG ATGCCACAAC TGTGACTAAT TCCGATGAGA AACCGCAGCC AGAGGCAGCG 
GCAGTGGCAG TAACAGAGGA GGAACCTGAT AAAGATCATT CCGTGACCAC TAATGGAGCG 
GACAATGCAG CAGCAGCACC ACCACCAACA GCTGCCAGTC CATCGAATCA CATCGAAGAG 
GGCGAAATAA CTGATAAGGA CGACGACGAC GATAATGTGC CGCCGCCTGT GCCTGTGCCT 
GTGCCCAAGC AGACGCTGGC CAGCGAAGTG CGCAAACGTA GTCGCAGTGG TGATGAAAAG 
AGCCGTAACC GGCGACGCAG TCCCAGCCGC AGTCGCCGTC GCACCCGTAC ACGCAGTCGC 
AGCAAGAGCC CGAAGCGTCG CCGCTCCAGC TCCTTGGAGC GGAGACGCCG CGAACGTCAG 
AAGCGGCACG AGGAGCGCGA TAAACGCGAT CAGGTGCGGG CGAGGGAGCG CGAAGATCGT 
CGTCGCAATG AAAAGTCGCG CAGCAGTCGG CGCGATGATT CTCGCGAGAG ATCCAGGCGG 
AGGTCCAGCG ACAAGCGGCG CTCCAGCTCG AGTGGCGGCT CCAAGAGGGC AGGTTCGGCA 
ATCGCCGACA ATGCCACGGT CACAAATCCC ACGGCCAAGA TCACAGAACG CCAGCGTCGC 
ACTGTGGATG TGCTGACATC GCGCACCGGA GGCGCTTACA TACCGCCAGC GAAGCTCCGG 
CTGCTGCAGG AGGGGATCAC GGACAAGGCA TCGGCTGCGT ATCAGCGCAT CGCCTGGGAG 
GCGCTCAAGA AGTCCATTCA CGGGTACATC AACAAGGTGA ATGTCACAAA TATTGCCATC 
ATTACCCGCG AACTCCTGCG GGAGAACATC GTCCGTGGAC GGGGCCTGCT GTCGAGGAGC 
ATCATCCAGG CCCAGGCCGC CTCGCCCACC TTCACGCACG TGTATGCCGC TCTGGTGGCC 
ATTATCAATT CGAAATTCCC GAATATCGGC GAGCTGCTGC TCAAGCGTCT GGTCATTCAG 
TTCCGCCGTG CCTTTCGGCG CAATGACAAG CTCGTCTGCT TGTCGTCCAC TCGATTCATT 
GCTCATCTGG TGAATCAGCG TGTGGCCCAC GAAATACTGG CCCTGGAGAT TCTCACGCTC 
CTGGTGGAGA CGCCCACCGA TGATTCCGTG GAGGTGGCCA TTGCCTTTCT CAAGGAGTGC 
GGCATGAAAC TGACGGAGGT CTCGTCCAAG GGCATTGGGG CCATTTTTGA GATGCTGCGC 
AACATCCTGC ACGAGGGAAA GCTGGACAAA CGAGTACAGT ACATGATCGA GGTGCTGTTT 
CAAGTGCGCA AGGATGGCTT CAAGGATCAT CTGGCCATTG TGGGAGAACT GGAGCTGGTC 
GAAGAGGACG AGCAGTTTAC GCATCTCATG ATGCTGGACG AGGCCACCGA AACGGAGGAT 
ATACTGAATG TCTTCAAATT TGACGAGAAC TTTGCCGAAA ACGAGGACAA ATACAAGGGT 
CTGAGTGCCG AAATCCTGGG CAGCGATGAT GGCAGCGGCT CGGGCTCTGG CTCTGGCTCG 
GGCTCCGATT CCGACAGCGA TTCGGATGGT GAAATCAACT CGGATGCCGA AGTCGGCAAA 
AAGACCGATG CCGGGGATAT TATCGACAAC ACGGAGACGA ATCTAATTGC TCTGCGGCGC 
ACCATATATC TGACGATCAA CTCGAGTTTG GATTACGAGG AGTGCGCCCA CAAGCTGATG 
AAAATGGAGC TGAAGCCGGG GCAGGAGGTG GAGCTGTGCC ACATGTTCCT CGACTGCTGT 
GCCGAGCAGC GGACCTACGA GAAGTTCTAC GGCCTGTTGG CGCAGCGTTT CTGCAACATC 
AATCGAATGT ATATACCCCC CTTCGAGGAG ATCTTCAAGG ACACCTACCA GACGACGCAT 
CGCCTGGACA CGAATCGGTT GCGGAATGTG AGCAAGTTCT TTGCCCATCT GCTCTTCACC 
GATTCCATCA GCTGGGATGT CTTGGACTGT GTCCTGCTGA ACGAGGACGA TACCACCTCA 
TCCAGTCGCA TTTTCATCAA GATTCTGTTC CAGGAGCTCG CCGAATACAT GGGCTTGGGC 
AAACTCAACT CCAAGCTCAA GGAAGAGGTG CTGGTGGAGA GCCTGGCGGG TCTCTTCCCC 
AAGGATAATC CTCGGAACAC ACGATTCTCC ATCAATTTCT TCACGTCCAT TGGTTTGGGT 
GGACTCACCG ATGATCTGCG ACGTTTCCTA AAGACAGCGC CCAAAGCAGT GCCCGCGATC 
AATTCAGAGC TCCTGTCCAC TGGGGGGAAT CCATTCCGGG ATCCAGGATC TGCCCCAGCA 
GCGGGGGCAG TACCCATTGC TGTGACTTCC TCCTCTGCCG GTTCGTCGAG CAGTTCCAGT 
AGCAGCAGCA GCGGAGACTC CAGCGAGCAA AAGTCGAGCA CCAGCGATGA GGACAGCGAC 
AGTGAATCCT CCAGCTCCGA GTCCGAGCCA CAGCGAAAGC GTAAACGCAA GGAGAAGCGC 
AAGAAGAAGC CCAAAAAGCA GCGAAAATCA GAGCTCAAAA AGTTAAAGGA CAAAAAAGAC 
AAAAAGAAGA AATCCAAAAA GGAAAAGGAT AAAGAGAAGG AGCGGGGGCG CGAGGAGAAA 
AAGAAGCGCG AAAAGGAGAA GAAAAAGGCC GCCAAAAAGA AGTCGAAACG CAAGCGCAAG 
CACCAGGAGA GCTCCAGTTC CAGCAGCAGC TCTGAAAGCA GCAAGGCCTC CTCCACATCC 
TCCTCAGCAT CAGAGGCCGC CTCGAGCAGC GACGACGACG ACAGCGATGG CCAGCCCCAG 
CCGAAGATCA TGCGCCAGGA GCAGAGCAGC AAAATCCACA ACAACCACAA CAGCAACAAC 
AAATTCAAGA CCCGAAAGGA AGACAGCGAT GGATTCAACC TGGAGGGACC ACCCATGGGC 
CGAGAACAGG AGCGCCAACC ACATTATCAT CATCAGCTCA ATGGAAATGG TGCCAGGAAG 
CGGGAGCCAT CGTTTTCTGC CCATGAGGAA AGACACCCGG AGCGGCGAGG GGAGCGAGAT 
GCCCGTCGTG GAGAATCCCG AGGTCTTCGT GGAGACTCCG GTGGCCGACG TGGAGACTCC 
GGTAGTCGTC GTGGAGAATC GCGAGGTCTT CGTGGAGACT CCGGTGGCCG TCGTCCAGAA 
ACCCGACGAG GCGAATCCCA AGGCCGCCGA AACGATAGAG GAGAACAACG TGGTGGCCGC 
CTCGGCAGTG ATCGTGATCG AGAACGTGAC AGAGGAGAAC GAGATCAGGA AAGAAATAGA 
GAACGTGAGC GGGAGCGGGA ACGGGATCAT GACCGTCGTG ACAATCGAGA CCGTGACCGT 
GATCGGGATC GTGATCACCC ACGTAATCGA GAACGTTCCC GTGACCAGGA TCGCGCCAGA 
AAACCAGAAC GAGAACGCAG CTCTCACAAG CGCGTGTCCA AGGGGCGCGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002014906.1
CDS	39..4052
Translation	MGESDAESDSCSGSSSSSGSSSSSSTSGSGSASKSDAGSRLASGQEENEEEERGQKAKEVAEEEVTPAEVSNDSPAVEEPPQKKEEEEKKVLQTDEKVASPKEEDEQKQTAAAKPEKQSNVDSSTAAVAVDTSVSAGVEKAEAEAVPTAAAPAEAAEKVEEEAITKSIEAETETETSTPQRPLDATTVTNSDEKPQPEAAAVAVTEEEPDKDHSVTTNGADNAAAAPPPTAASPSNHIEEGEITDKDDDDDNVPPPVPVPVPKQTLASEVRKRSRSGDEKSRNRRRSPSRSRRRTRTRSRSKSPKRRRSSSLERRRRERQKRHEERDKRDQVRAREREDRRRNEKSRSSRRDDSRERSRRRSSDKRRSSSSGGSKRAGSAIADNATVTNPTAKITERQRRTVDVLTSRTGGAYIPPAKLRLLQEGITDKASAAYQRIAWEALKKSIHGYINKVNVTNIAIITRELLRENIVRGRGLLSRSIIQAQAASPTFTHVYAALVAIINSKFPNIGELLLKRLVIQFRRAFRRNDKLVCLSSTRFIAHLVNQRVAHEILALEILTLLVETPTDDSVEVAIAFLKECGMKLTEVSSKGIGAIFEMLRNILHEGKLDKRVQYMIEVLFQVRKDGFKDHLAIVGELELVEEDEQFTHLMMLDEATETEDILNVFKFDENFAENEDKYKGLSAEILGSDDGSGSGSGSGSGSDSDSDSDGEINSDAEVGKKTDAGDIIDNTETNLIALRRTIYLTINSSLDYEECAHKLMKMELKPGQEVELCHMFLDCCAEQRTYEKFYGLLAQRFCNINRMYIPPFEEIFKDTYQTTHRLDTNRLRNVSKFFAHLLFTDSISWDVLDCVLLNEDDTTSSSRIFIKILFQELAEYMGLGKLNSKLKEEVLVESLAGLFPKDNPRNTRFSINFFTSIGLGGLTDDLRRFLKTAPKAVPAINSELLSTGGNPFRDPGSAPAAGAVPIAVTSSSAGSSSSSSSSSSGDSSEQKSSTSDEDSDSESSSSESEPQRKRKRKEKRKKKPKKQRKSELKKLKDKKDKKKKSKKEKDKEKERGREEKKKREKEKKKAAKKKSKRKRKHQESSSSSSSSESSKASSTSSSASEAASSSDDDDSDGQPQPKIMRQEQSSKIHNNHNSNNKFKTRKEDSDGFNLEGPPMGREQERQPHYHHQLNGNGARKREPSFSAHEERHPERRGERDARRGESRGLRGDSGGRRGDSGSRRGESRGLRGDSGGRRPETRRGESQGRRNDRGEQRGGRLGSDRDRERDRGERDQERNRERERERERDHDRRDNRDRDRDRDRDHPRNRERSRDQDRARKPERERSSHKRVSKGRG

Target ORF information:

RefSeq Version	XM_002014870.2
Organism	Drosophila persimilis
Definition	Drosophila persimilis pre-mRNA-splicing factor CWC22 homolog (LOC6588851), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002014870.2

ORF Insert Sequence:

ATGGGGGAAA GCGATGCAGA AAGTGATTCG TGCAGTGGCT CCAGTAGTAG CAGTGGCTCC 
TCGAGCAGTT CCTCCACCAG TGGCAGCGGC AGTGCCTCCA AAAGTGATGC TGGTAGCCGT 
CTGGCAAGCG GTCAGGAGGA AAACGAAGAG GAAGAGCGCG GCCAGAAGGC AAAAGAAGTG 
GCAGAAGAAG AAGTGACGCC GGCAGAAGTA TCTAATGATT CCCCCGCCGT AGAGGAGCCG 
CCGCAAAAGA AGGAGGAGGA AGAAAAGAAA GTGCTACAAA CAGACGAAAA AGTTGCCTCT 
CCAAAAGAGG AGGACGAGCA AAAGCAAACC GCAGCAGCAA AGCCGGAAAA ACAATCAAAT 
GTTGACTCTT CCACAGCTGC TGTTGCTGTT GACACGTCTG TGTCGGCTGG CGTGGAAAAA 
GCAGAAGCAG AAGCAGTACC GACAGCAGCG GCGCCTGCAG AGGCAGCAGA AAAAGTAGAA 
GAGGAAGCTA TAACAAAATC AATTGAAGCG GAAACAGAAA CCGAAACATC TACCCCGCAA 
CGACCCTTGG ATGCCACAAC TGTGACTAAT TCCGATGAGA AACCGCAGCC AGAGGCAGCG 
GCAGTGGCAG TAACAGAGGA GGAACCTGAT AAAGATCATT CCGTGACCAC TAATGGAGCG 
GACAATGCAG CAGCAGCACC ACCACCAACA GCTGCCAGTC CATCGAATCA CATCGAAGAG 
GGCGAAATAA CTGATAAGGA CGACGACGAC GATAATGTGC CGCCGCCTGT GCCTGTGCCT 
GTGCCCAAGC AGACGCTGGC CAGCGAAGTG CGCAAACGTA GTCGCAGTGG TGATGAAAAG 
AGCCGTAACC GGCGACGCAG TCCCAGCCGC AGTCGCCGTC GCACCCGTAC ACGCAGTCGC 
AGCAAGAGCC CGAAGCGTCG CCGCTCCAGC TCCTTGGAGC GGAGACGCCG CGAACGTCAG 
AAGCGGCACG AGGAGCGCGA TAAACGCGAT CAGGTGCGGG CGAGGGAGCG CGAAGATCGT 
CGTCGCAATG AAAAGTCGCG CAGCAGTCGG CGCGATGATT CTCGCGAGAG ATCCAGGCGG 
AGGTCCAGCG ACAAGCGGCG CTCCAGCTCG AGTGGCGGCT CCAAGAGGGC AGGTTCGGCA 
ATCGCCGACA ATGCCACGGT CACAAATCCC ACGGCCAAGA TCACAGAACG CCAGCGTCGC 
ACTGTGGATG TGCTGACATC GCGCACCGGA GGCGCTTACA TACCGCCAGC GAAGCTCCGG 
CTGCTGCAGG AGGGGATCAC GGACAAGGCA TCGGCTGCGT ATCAGCGCAT CGCCTGGGAG 
GCGCTCAAGA AGTCCATTCA CGGGTACATC AACAAGGTGA ATGTCACAAA TATTGCCATC 
ATTACCCGCG AACTCCTGCG GGAGAACATC GTCCGTGGAC GGGGCCTGCT GTCGAGGAGC 
ATCATCCAGG CCCAGGCCGC CTCGCCCACC TTCACGCACG TGTATGCCGC TCTGGTGGCC 
ATTATCAATT CGAAATTCCC GAATATCGGC GAGCTGCTGC TCAAGCGTCT GGTCATTCAG 
TTCCGCCGTG CCTTTCGGCG CAATGACAAG CTCGTCTGCT TGTCGTCCAC TCGATTCATT 
GCTCATCTGG TGAATCAGCG TGTGGCCCAC GAAATACTGG CCCTGGAGAT TCTCACGCTC 
CTGGTGGAGA CGCCCACCGA TGATTCCGTG GAGGTGGCCA TTGCCTTTCT CAAGGAGTGC 
GGCATGAAAC TGACGGAGGT CTCGTCCAAG GGCATTGGGG CCATTTTTGA GATGCTGCGC 
AACATCCTGC ACGAGGGAAA GCTGGACAAA CGAGTACAGT ACATGATCGA GGTGCTGTTT 
CAAGTGCGCA AGGATGGCTT CAAGGATCAT CTGGCCATTG TGGGAGAACT GGAGCTGGTC 
GAAGAGGACG AGCAGTTTAC GCATCTCATG ATGCTGGACG AGGCCACCGA AACGGAGGAT 
ATACTGAATG TCTTCAAATT TGACGAGAAC TTTGCCGAAA ACGAGGACAA ATACAAGGGT 
CTGAGTGCCG AAATCCTGGG CAGCGATGAT GGCAGCGGCT CGGGCTCTGG CTCTGGCTCG 
GGCTCCGATT CCGACAGCGA TTCGGATGGT GAAATCAACT CGGATGCCGA AGTCGGCAAA 
AAGACCGATG CCGGGGATAT TATCGACAAC ACGGAGACGA ATCTAATTGC TCTGCGGCGC 
ACCATATATC TGACGATCAA CTCGAGTTTG GATTACGAGG AGTGCGCCCA CAAGCTGATG 
AAAATGGAGC TGAAGCCGGG GCAGGAGGTG GAGCTGTGCC ACATGTTCCT CGACTGCTGT 
GCCGAGCAGC GGACCTACGA GAAGTTCTAC GGCCTGTTGG CGCAGCGTTT CTGCAACATC 
AATCGAATGT ATATACCCCC CTTCGAGGAG ATCTTCAAGG ACACCTACCA GACGACGCAT 
CGCCTGGACA CGAATCGGTT GCGGAATGTG AGCAAGTTCT TTGCCCATCT GCTCTTCACC 
GATTCCATCA GCTGGGATGT CTTGGACTGT GTCCTGCTGA ACGAGGACGA TACCACCTCA 
TCCAGTCGCA TTTTCATCAA GATTCTGTTC CAGGAGCTCG CCGAATACAT GGGCTTGGGC 
AAACTCAACT CCAAGCTCAA GGAAGAGGTG CTGGTGGAGA GCCTGGCGGG TCTCTTCCCC 
AAGGATAATC CTCGGAACAC ACGATTCTCC ATCAATTTCT TCACGTCCAT TGGTTTGGGT 
GGACTCACCG ATGATCTGCG ACGTTTCCTA AAGACAGCGC CCAAAGCAGT GCCCGCGATC 
AATTCAGAGC TCCTGTCCAC TGGGGGGAAT CCATTCCGGG ATCCAGGATC TGCCCCAGCA 
GCGGGGGCAG TACCCATTGC TGTGACTTCC TCCTCTGCCG GTTCGTCGAG CAGTTCCAGT 
AGCAGCAGCA GCGGAGACTC CAGCGAGCAA AAGTCGAGCA CCAGCGATGA GGACAGCGAC 
AGTGAATCCT CCAGCTCCGA GTCCGAGCCA CAGCGAAAGC GTAAACGCAA GGAGAAGCGC 
AAGAAGAAGC CCAAAAAGCA GCGAAAATCA GAGCTCAAAA AGTTAAAGGA CAAAAAAGAC 
AAAAAGAAGA AATCCAAAAA GGAAAAGGAT AAAGAGAAGG AGCGGGGGCG CGAGGAGAAA 
AAGAAGCGCG AAAAGGAGAA GAAAAAGGCC GCCAAAAAGA AGTCGAAACG CAAGCGCAAG 
CACCAGGAGA GCTCCAGTTC CAGCAGCAGC TCTGAAAGCA GCAAGGCCTC CTCCACATCC 
TCCTCAGCAT CAGAGGCCGC CTCGAGCAGC GACGACGACG ACAGCGATGG CCAGCCCCAG 
CCGAAGATCA TGCGCCAGGA GCAGAGCAGC AAAATCCACA ACAACCACAA CAGCAACAAC 
AAATTCAAGA CCCGAAAGGA AGACAGCGAT GGATTCAACC TGGAGGGACC ACCCATGGGC 
CGAGAACAGG AGCGCCAACC ACATTATCAT CATCAGCTCA ATGGAAATGG TGCCAGGAAG 
CGGGAGCCAT CGTTTTCTGC CCATGAGGAA AGACACCCGG AGCGGCGAGG GGAGCGAGAT 
GCCCGTCGTG GAGAATCCCG AGGTCTTCGT GGAGACTCCG GTGGCCGACG TGGAGACTCC 
GGTAGTCGTC GTGGAGAATC GCGAGGTCTT CGTGGAGACT CCGGTGGCCG TCGTCCAGAA 
ACCCGACGAG GCGAATCCCA AGGCCGCCGA AACGATAGAG GAGAACAACG TGGTGGCCGC 
CTCGGCAGTG ATCGTGATCG AGAACGTGAC AGAGGAGAAC GAGATCAGGA AAGAAATAGA 
GAACGTGAGC GGGAGCGGGA ACGGGATCAT GACCGTCGTG ACAATCGAGA CCGTGACCGT 
GATCGGGATC GTGATCACCC ACGTAATCGA GAACGTTCCC GTGACCAGGA TCGCGCCAGA 
AAACCAGAAC GAGAACGCAG CTCTCACAAG CGCGTGTCCA AGGGGCGCGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6588851 ( XM_026988482.1 ) cDNA ORF clone, Drosophila persimilis -> XP_026844283.1 Drosophila persimilis pre-mRNA-splicing factor CWC22 homolog (LOC6588851), transcript variant X1, mRNA.

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CloneID	ODb22428
Clone ID Related Accession (Same CDS sequence)	XM_026988482.1
Accession Version	XM_026988482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541433600000
Organism	Drosophila persimilis
Product	pre-mRNA-splicing factor CWC22 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825298.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila persimilis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAA GCGATGCAGA AAGTGATTCG TGCAGTGGCT CCAGTAGTAG CAGTGGCTCC 
TCGAGCAGTT CCTCCACCAG TGGCAGCGGC AGTGCCTCCA AAAGTGATGC TGGTAGCCGT 
CTGGCAAGCG GTCAGGAGGA AAACGAAGAG GAAGAGCGCG GCCAGAAGGC AAAAGAAGTG 
GCAGAAGAAG AAGTGACGCC GGCAGAAGTA TCTAATGATT CCCCCGCCGT AGAGGAGCCG 
CCGCAAAAGA AGGAGGAGGA AGAAAAGAAA GTGCTACAAA CAGACGAAAA AGTTGCCTCT 
CCAAAAGAGG AGGACGAGCA AAAGCAAACC GCAGCAGCAA AGCCGGAAAA ACAATCAAAT 
GTTGACTCTT CCACAGCTGC TGTTGCTGTT GACACGTCTG TGTCGGCTGG CGTGGAAAAA 
GCAGAAGCAG AAGCAGTACC GACAGCAGCG GCGCCTGCAG AGGCAGCAGA AAAAGTAGAA 
GAGGAAGCTA TAACAAAATC AATTGAAGCG GAAACAGAAA CCGAAACATC TACCCCGCAA 
CGACCCTTGG ATGCCACAAC TGTGACTAAT TCCGATGAGA AACCGCAGCC AGAGGCAGCG 
GCAGTGGCAG TAACAGAGGA GGAACCTGAT AAAGATCATT CCGTGACCAC TAATGGAGCG 
GACAATGCAG CAGCAGCACC ACCACCAACA GCTGCCAGTC CATCGAATCA CATCGAAGAG 
GGCGAAATAA CTGATGTAAG TTTTGATCAA ACAAAACTAC AAGCCGCTGA AAAAAACTCA 
TGGCAATCCC TTCCACAGAA GGACGACGAC GACGATAATG TGCCGCCGCC TGTGCCTGTG 
CCTGTGCCCA AGCAGACGCT GGCCAGCGAA GTGCGCAAAC GTAGTCGCAG TGGTGATGAA 
AAGAGCCGTA ACCGGCGACG CAGTCCCAGC CGCAGTCGCC GTCGCACCCG TACACGCAGT 
CGCAGCAAGA GCCCGAAGCG TCGCCGCTCC AGCTCCTTGG AGCGGAGACG CCGCGAACGT 
CAGAAGCGGC ACGAGGAGCG CGATAAACGC GATCAGGTGC GGGCGAGGGA GCGCGAAGAT 
CGTCGTCGCA ATGAAAAGTC GCGCAGCAGT CGGCGCGATG ATTCTCGCGA GAGATCCAGG 
CGGAGGTCCA GCGACAAGCG GCGCTCCAGC TCGAGTGGCG GCTCCAAGAG GGCAGGTTCG 
GCAATCGCCG ACAATGCCAC GGTCACAAAT CCCACGGCCA AGATCACAGA ACGCCAGCGT 
CGCACTGTGG ATGTGCTGAC ATCGCGCACC GGAGGCGCTT ACATACCGCC AGCGAAGCTC 
CGGCTGCTGC AGGAGGGGAT CACGGACAAG GCATCGGCTG CGTATCAGCG CATCGCCTGG 
GAGGCGCTCA AGAAGTCCAT TCACGGGTAC ATCAACAAGG TGAATGTCAC AAATATTGCC 
ATCATTACCC GCGAACTCCT GCGGGAGAAC ATCGTCCGTG GACGGGGCCT GCTGTCGAGG 
AGCATCATCC AGGCCCAGGC CGCCTCGCCC ACCTTCACGC ACGTGTATGC CGCTCTGGTG 
GCCATTATCA ATTCGAAATT CCCGAATATC GGCGAGCTGC TGCTCAAGCG TCTGGTCATT 
CAGTTCCGCC GTGCCTTTCG GCGCAATGAC AAGCTCGTCT GCTTGTCGTC CACTCGATTC 
ATTGCTCATC TGGTGAATCA GCGTGTGGCC CACGAAATAC TGGCCCTGGA GATTCTCACG 
CTCCTGGTGG AGACGCCCAC CGATGATTCC GTGGAGGTGG CCATTGCCTT TCTCAAGGAG 
TGCGGCATGA AACTGACGGA GGTCTCGTCC AAGGGCATTG GGGCCATTTT TGAGATGCTG 
CGCAACATCC TGCACGAGGG AAAGCTGGAC AAACGAGTAC AGTACATGAT CGAGGTGCTG 
TTTCAAGTGC GCAAGGATGG CTTCAAGGAT CATCTGGCCA TTGTGGGAGA ACTGGAGCTG 
GTCGAAGAGG ACGAGCAGTT TACGCATCTC ATGATGCTGG ACGAGGCCAC CGAAACGGAG 
GATATACTGA ATGTCTTCAA ATTTGACGAG AACTTTGCCG AAAACGAGGA CAAATACAAG 
GGTCTGAGTG CCGAAATCCT GGGCAGCGAT GATGGCAGCG GCTCGGGCTC TGGCTCTGGC 
TCGGGCTCCG ATTCCGACAG CGATTCGGAT GGTGAAATCA ACTCGGATGC CGAAGTCGGC 
AAAAAGACCG ATGCCGGGGA TATTATCGAC AACACGGAGA CGAATCTAAT TGCTCTGCGG 
CGCACCATAT ATCTGACGAT CAACTCGAGT TTGGATTACG AGGAGTGCGC CCACAAGCTG 
ATGAAAATGG AGCTGAAGCC GGGGCAGGAG GTGGAGCTGT GCCACATGTT CCTCGACTGC 
TGTGCCGAGC AGCGGACCTA CGAGAAGTTC TACGGCCTGT TGGCGCAGCG TTTCTGCAAC 
ATCAATCGAA TGTATATACC CCCCTTCGAG GAGATCTTCA AGGACACCTA CCAGACGACG 
CATCGCCTGG ACACGAATCG GTTGCGGAAT GTGAGCAAGT TCTTTGCCCA TCTGCTCTTC 
ACCGATTCCA TCAGCTGGGA TGTCTTGGAC TGTGTCCTGC TGAACGAGGA CGATACCACC 
TCATCCAGTC GCATTTTCAT CAAGATTCTG TTCCAGGAGC TCGCCGAATA CATGGGCTTG 
GGCAAACTCA ACTCCAAGCT CAAGGAAGAG GTGCTGGTGG AGAGCCTGGC GGGTCTCTTC 
CCCAAGGATA ATCCTCGGAA CACACGATTC TCCATCAATT TCTTCACGTC CATTGGTTTG 
GGTGGACTCA CCGATGATCT GCGACGTTTC CTAAAGACAG CGCCCAAAGC AGTGCCCGCG 
ATCAATTCAG AGCTCCTGTC CACTGGGGGG AATCCATTCC GGGATCCAGG ATCTGCCCCA 
GCAGCGGGGG CAGTACCCAT TGCTGTGACT TCCTCCTCTG CCGGTTCGTC GAGCAGTTCC 
AGTAGCAGCA GCAGCGGAGA CTCCAGCGAG CAAAAGTCGA GCACCAGCGA TGAGGACAGC 
GACAGTGAAT CCTCCAGCTC CGAGTCCGAG CCACAGCGAA AGCGTAAACG CAAGGAGAAG 
CGCAAGAAGA AGCCCAAAAA GCAGCGAAAA TCAGAGCTCA AAAAGTTAAA GGACAAAAAA 
GACAAAAAGA AGAAATCCAA AAAGGAAAAG GATAAAGAGA AGGAGCGGGG GCGCGAGGAG 
AAAAAGAAGC GCGAAAAGGA GAAGAAAAAG GCCGCCAAAA AGAAGTCGAA ACGCAAGCGC 
AAGCACCAGG AGAGCTCCAG TTCCAGCAGC AGCTCTGAAA GCAGCAAGGC CTCCTCCACA 
TCCTCCTCAG CATCAGAGGC CGCCTCGAGC AGCGACGACG ACGACAGCGA TGGCCAGCCC 
CAGCCGAAGA TCATGCGCCA GGAGCAGAGC AGCAAAATCC ACAACAACCA CAACAGCAAC 
AACAAATTCA AGACCCGAAA GGAAGACAGC GATGGATTCA ACCTGGAGGG ACCACCCATG 
GGCCGAGAAC AGGAGCGCCA ACCACATTAT CATCATCAGC TCAATGGAAA TGGTGCCAGG 
AAGCGGGAGC CATCGTTTTC TGCCCATGAG GAAAGACACC CGGAGCGGCG AGGGGAGCGA 
GATGCCCGTC GTGGAGAATC CCGAGGTCTT CGTGGAGACT CCGGTGGCCG ACGTGGAGAC 
TCCGGTAGTC GTCGTGGAGA ATCGCGAGGT CTTCGTGGAG ACTCCGGTGG CCGTCGTCCA 
GAAACCCGAC GAGGCGAATC CCAAGGCCGC CGAAACGATA GAGGAGAACA ACGTGGTGGC 
CGCCTCGGCA GTGATCGTGA TCGAGAACGT GACAGAGGAG AACGAGATCA GGAAAGAAAT 
AGAGAACGTG AGCGGGAGCG GGAACGGGAT CATGACCGTC GTGACAATCG AGACCGTGAC 
CGTGATCGGG ATCGTGATCA CCCACGTAAT CGAGAACGTT CCCGTGACCA GGATCGCGCC 
AGAAAACCAG AACGAGAACG CAGCTCTCAC AAGCGCGTGT CCAAGGGGCG CGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026844283.1
CDS	41..4117
Translation	MGESDAESDSCSGSSSSSGSSSSSSTSGSGSASKSDAGSRLASGQEENEEEERGQKAKEVAEEEVTPAEVSNDSPAVEEPPQKKEEEEKKVLQTDEKVASPKEEDEQKQTAAAKPEKQSNVDSSTAAVAVDTSVSAGVEKAEAEAVPTAAAPAEAAEKVEEEAITKSIEAETETETSTPQRPLDATTVTNSDEKPQPEAAAVAVTEEEPDKDHSVTTNGADNAAAAPPPTAASPSNHIEEGEITDVSFDQTKLQAAEKNSWQSLPQKDDDDDNVPPPVPVPVPKQTLASEVRKRSRSGDEKSRNRRRSPSRSRRRTRTRSRSKSPKRRRSSSLERRRRERQKRHEERDKRDQVRAREREDRRRNEKSRSSRRDDSRERSRRRSSDKRRSSSSGGSKRAGSAIADNATVTNPTAKITERQRRTVDVLTSRTGGAYIPPAKLRLLQEGITDKASAAYQRIAWEALKKSIHGYINKVNVTNIAIITRELLRENIVRGRGLLSRSIIQAQAASPTFTHVYAALVAIINSKFPNIGELLLKRLVIQFRRAFRRNDKLVCLSSTRFIAHLVNQRVAHEILALEILTLLVETPTDDSVEVAIAFLKECGMKLTEVSSKGIGAIFEMLRNILHEGKLDKRVQYMIEVLFQVRKDGFKDHLAIVGELELVEEDEQFTHLMMLDEATETEDILNVFKFDENFAENEDKYKGLSAEILGSDDGSGSGSGSGSGSDSDSDSDGEINSDAEVGKKTDAGDIIDNTETNLIALRRTIYLTINSSLDYEECAHKLMKMELKPGQEVELCHMFLDCCAEQRTYEKFYGLLAQRFCNINRMYIPPFEEIFKDTYQTTHRLDTNRLRNVSKFFAHLLFTDSISWDVLDCVLLNEDDTTSSSRIFIKILFQELAEYMGLGKLNSKLKEEVLVESLAGLFPKDNPRNTRFSINFFTSIGLGGLTDDLRRFLKTAPKAVPAINSELLSTGGNPFRDPGSAPAAGAVPIAVTSSSAGSSSSSSSSSSGDSSEQKSSTSDEDSDSESSSSESEPQRKRKRKEKRKKKPKKQRKSELKKLKDKKDKKKKSKKEKDKEKERGREEKKKREKEKKKAAKKKSKRKRKHQESSSSSSSSESSKASSTSSSASEAASSSDDDDSDGQPQPKIMRQEQSSKIHNNHNSNNKFKTRKEDSDGFNLEGPPMGREQERQPHYHHQLNGNGARKREPSFSAHEERHPERRGERDARRGESRGLRGDSGGRRGDSGSRRGESRGLRGDSGGRRPETRRGESQGRRNDRGEQRGGRLGSDRDRERDRGERDQERNRERERERERDHDRRDNRDRDRDRDRDHPRNRERSRDQDRARKPERERSSHKRVSKGRG

Target ORF information:

RefSeq Version	XM_026988482.1
Organism	Drosophila persimilis
Definition	Drosophila persimilis pre-mRNA-splicing factor CWC22 homolog (LOC6588851), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026988482.1

ORF Insert Sequence:

ATGGGGGAAA GCGATGCAGA AAGTGATTCG TGCAGTGGCT CCAGTAGTAG CAGTGGCTCC 
TCGAGCAGTT CCTCCACCAG TGGCAGCGGC AGTGCCTCCA AAAGTGATGC TGGTAGCCGT 
CTGGCAAGCG GTCAGGAGGA AAACGAAGAG GAAGAGCGCG GCCAGAAGGC AAAAGAAGTG 
GCAGAAGAAG AAGTGACGCC GGCAGAAGTA TCTAATGATT CCCCCGCCGT AGAGGAGCCG 
CCGCAAAAGA AGGAGGAGGA AGAAAAGAAA GTGCTACAAA CAGACGAAAA AGTTGCCTCT 
CCAAAAGAGG AGGACGAGCA AAAGCAAACC GCAGCAGCAA AGCCGGAAAA ACAATCAAAT 
GTTGACTCTT CCACAGCTGC TGTTGCTGTT GACACGTCTG TGTCGGCTGG CGTGGAAAAA 
GCAGAAGCAG AAGCAGTACC GACAGCAGCG GCGCCTGCAG AGGCAGCAGA AAAAGTAGAA 
GAGGAAGCTA TAACAAAATC AATTGAAGCG GAAACAGAAA CCGAAACATC TACCCCGCAA 
CGACCCTTGG ATGCCACAAC TGTGACTAAT TCCGATGAGA AACCGCAGCC AGAGGCAGCG 
GCAGTGGCAG TAACAGAGGA GGAACCTGAT AAAGATCATT CCGTGACCAC TAATGGAGCG 
GACAATGCAG CAGCAGCACC ACCACCAACA GCTGCCAGTC CATCGAATCA CATCGAAGAG 
GGCGAAATAA CTGATGTAAG TTTTGATCAA ACAAAACTAC AAGCCGCTGA AAAAAACTCA 
TGGCAATCCC TTCCACAGAA GGACGACGAC GACGATAATG TGCCGCCGCC TGTGCCTGTG 
CCTGTGCCCA AGCAGACGCT GGCCAGCGAA GTGCGCAAAC GTAGTCGCAG TGGTGATGAA 
AAGAGCCGTA ACCGGCGACG CAGTCCCAGC CGCAGTCGCC GTCGCACCCG TACACGCAGT 
CGCAGCAAGA GCCCGAAGCG TCGCCGCTCC AGCTCCTTGG AGCGGAGACG CCGCGAACGT 
CAGAAGCGGC ACGAGGAGCG CGATAAACGC GATCAGGTGC GGGCGAGGGA GCGCGAAGAT 
CGTCGTCGCA ATGAAAAGTC GCGCAGCAGT CGGCGCGATG ATTCTCGCGA GAGATCCAGG 
CGGAGGTCCA GCGACAAGCG GCGCTCCAGC TCGAGTGGCG GCTCCAAGAG GGCAGGTTCG 
GCAATCGCCG ACAATGCCAC GGTCACAAAT CCCACGGCCA AGATCACAGA ACGCCAGCGT 
CGCACTGTGG ATGTGCTGAC ATCGCGCACC GGAGGCGCTT ACATACCGCC AGCGAAGCTC 
CGGCTGCTGC AGGAGGGGAT CACGGACAAG GCATCGGCTG CGTATCAGCG CATCGCCTGG 
GAGGCGCTCA AGAAGTCCAT TCACGGGTAC ATCAACAAGG TGAATGTCAC AAATATTGCC 
ATCATTACCC GCGAACTCCT GCGGGAGAAC ATCGTCCGTG GACGGGGCCT GCTGTCGAGG 
AGCATCATCC AGGCCCAGGC CGCCTCGCCC ACCTTCACGC ACGTGTATGC CGCTCTGGTG 
GCCATTATCA ATTCGAAATT CCCGAATATC GGCGAGCTGC TGCTCAAGCG TCTGGTCATT 
CAGTTCCGCC GTGCCTTTCG GCGCAATGAC AAGCTCGTCT GCTTGTCGTC CACTCGATTC 
ATTGCTCATC TGGTGAATCA GCGTGTGGCC CACGAAATAC TGGCCCTGGA GATTCTCACG 
CTCCTGGTGG AGACGCCCAC CGATGATTCC GTGGAGGTGG CCATTGCCTT TCTCAAGGAG 
TGCGGCATGA AACTGACGGA GGTCTCGTCC AAGGGCATTG GGGCCATTTT TGAGATGCTG 
CGCAACATCC TGCACGAGGG AAAGCTGGAC AAACGAGTAC AGTACATGAT CGAGGTGCTG 
TTTCAAGTGC GCAAGGATGG CTTCAAGGAT CATCTGGCCA TTGTGGGAGA ACTGGAGCTG 
GTCGAAGAGG ACGAGCAGTT TACGCATCTC ATGATGCTGG ACGAGGCCAC CGAAACGGAG 
GATATACTGA ATGTCTTCAA ATTTGACGAG AACTTTGCCG AAAACGAGGA CAAATACAAG 
GGTCTGAGTG CCGAAATCCT GGGCAGCGAT GATGGCAGCG GCTCGGGCTC TGGCTCTGGC 
TCGGGCTCCG ATTCCGACAG CGATTCGGAT GGTGAAATCA ACTCGGATGC CGAAGTCGGC 
AAAAAGACCG ATGCCGGGGA TATTATCGAC AACACGGAGA CGAATCTAAT TGCTCTGCGG 
CGCACCATAT ATCTGACGAT CAACTCGAGT TTGGATTACG AGGAGTGCGC CCACAAGCTG 
ATGAAAATGG AGCTGAAGCC GGGGCAGGAG GTGGAGCTGT GCCACATGTT CCTCGACTGC 
TGTGCCGAGC AGCGGACCTA CGAGAAGTTC TACGGCCTGT TGGCGCAGCG TTTCTGCAAC 
ATCAATCGAA TGTATATACC CCCCTTCGAG GAGATCTTCA AGGACACCTA CCAGACGACG 
CATCGCCTGG ACACGAATCG GTTGCGGAAT GTGAGCAAGT TCTTTGCCCA TCTGCTCTTC 
ACCGATTCCA TCAGCTGGGA TGTCTTGGAC TGTGTCCTGC TGAACGAGGA CGATACCACC 
TCATCCAGTC GCATTTTCAT CAAGATTCTG TTCCAGGAGC TCGCCGAATA CATGGGCTTG 
GGCAAACTCA ACTCCAAGCT CAAGGAAGAG GTGCTGGTGG AGAGCCTGGC GGGTCTCTTC 
CCCAAGGATA ATCCTCGGAA CACACGATTC TCCATCAATT TCTTCACGTC CATTGGTTTG 
GGTGGACTCA CCGATGATCT GCGACGTTTC CTAAAGACAG CGCCCAAAGC AGTGCCCGCG 
ATCAATTCAG AGCTCCTGTC CACTGGGGGG AATCCATTCC GGGATCCAGG ATCTGCCCCA 
GCAGCGGGGG CAGTACCCAT TGCTGTGACT TCCTCCTCTG CCGGTTCGTC GAGCAGTTCC 
AGTAGCAGCA GCAGCGGAGA CTCCAGCGAG CAAAAGTCGA GCACCAGCGA TGAGGACAGC 
GACAGTGAAT CCTCCAGCTC CGAGTCCGAG CCACAGCGAA AGCGTAAACG CAAGGAGAAG 
CGCAAGAAGA AGCCCAAAAA GCAGCGAAAA TCAGAGCTCA AAAAGTTAAA GGACAAAAAA 
GACAAAAAGA AGAAATCCAA AAAGGAAAAG GATAAAGAGA AGGAGCGGGG GCGCGAGGAG 
AAAAAGAAGC GCGAAAAGGA GAAGAAAAAG GCCGCCAAAA AGAAGTCGAA ACGCAAGCGC 
AAGCACCAGG AGAGCTCCAG TTCCAGCAGC AGCTCTGAAA GCAGCAAGGC CTCCTCCACA 
TCCTCCTCAG CATCAGAGGC CGCCTCGAGC AGCGACGACG ACGACAGCGA TGGCCAGCCC 
CAGCCGAAGA TCATGCGCCA GGAGCAGAGC AGCAAAATCC ACAACAACCA CAACAGCAAC 
AACAAATTCA AGACCCGAAA GGAAGACAGC GATGGATTCA ACCTGGAGGG ACCACCCATG 
GGCCGAGAAC AGGAGCGCCA ACCACATTAT CATCATCAGC TCAATGGAAA TGGTGCCAGG 
AAGCGGGAGC CATCGTTTTC TGCCCATGAG GAAAGACACC CGGAGCGGCG AGGGGAGCGA 
GATGCCCGTC GTGGAGAATC CCGAGGTCTT CGTGGAGACT CCGGTGGCCG ACGTGGAGAC 
TCCGGTAGTC GTCGTGGAGA ATCGCGAGGT CTTCGTGGAG ACTCCGGTGG CCGTCGTCCA 
GAAACCCGAC GAGGCGAATC CCAAGGCCGC CGAAACGATA GAGGAGAACA ACGTGGTGGC 
CGCCTCGGCA GTGATCGTGA TCGAGAACGT GACAGAGGAG AACGAGATCA GGAAAGAAAT 
AGAGAACGTG AGCGGGAGCG GGAACGGGAT CATGACCGTC GTGACAATCG AGACCGTGAC 
CGTGATCGGG ATCGTGATCA CCCACGTAAT CGAGAACGTT CCCGTGACCA GGATCGCGCC 
AGAAAACCAG AACGAGAACG CAGCTCTCAC AAGCGCGTGT CCAAGGGGCG CGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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LOC6588851 cDNA ORF clone, Drosophila persimilis

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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