Dbp45A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dbp45A
Entrez Gene ID	35917
Full Name	DEAD box protein 45A
Synonyms	CG12759,DBP45A,Dbp45,DmRH18,Dmel\CG12759,dbp45A
General protein information	Preferred Names DEAD box protein 45A Names CG12759 gene product from transcript CG12759-RA ATP-dependent RNA helicase CG12759-PA Dbp45A-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45A1-45A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057579.5	NP_476927.1	DEAD box protein 45A

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A05203g	XP_451229.1
Eremothecium gossypii	AGOS_AER301C	NP_985158.1
Rattus norvegicus (Norway rat)	Ddx49	NP_001108495.1
Danio rerio (zebrafish)	ddx49	NP_938179.1
Xenopus tropicalis (tropical clawed frog)	ddx49	NP_989409.1
Oryza sativa (rice)	Os07g0633500	NP_001060374.1
Neurospora crassa	NCU06418	XP_957309.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005130	XP_314012.3
Homo sapiens (human)	DDX49	NP_061943.2
Macaca mulatta (Rhesus monkey)	DDX49	XP_001115617.1
Arabidopsis thaliana (thale cress)	RH36	NP_173078.1
Magnaporthe oryzae (rice blast fungus)	MGG_06483	XP_003717078.1
Pan troglodytes (chimpanzee)	DDX49	NP_001233517.1
Gallus gallus (chicken)	DDX49	NP_001026109.1
Caenorhabditis elegans (roundworm)	H20J04.4	NP_001040789.1
Schizosaccharomyces pombe (fission yeast)	dbp8	NP_596794.1
Canis lupus familiaris (dog)	DDX49	XP_541930.2
Bos taurus (cattle)	DDX49	NP_001075198.1
Mus musculus (house mouse)	Ddx49	NP_001020093.2
Drosophila melanogaster (fruit fly)	Dbp45A	NP_476927.1
Saccharomyces cerevisiae (baker's yeast)	DBP8	NP_012039.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dbp45A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dbp45A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09320	NM_057579.5 Latest version!	Drosophila melanogaster DEAD box protein 45A (Dbp45A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dbp45A ( NM_057579.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476927.1 Drosophila melanogaster DEAD box protein 45A (Dbp45A), mRNA.

Price & Availability↑

CloneID	OFa09320
Clone ID Related Accession (Same CDS sequence)	NM_057579.5
Accession Version	NM_057579.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	DEAD box protein 45A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057579.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACGAA AGGAGGCCAA TCCCTTTCAG ATCCTGGGCC TACGTCCATG GTTGGTTAAG 
CAGCTGACCA AGTTGGGCCT GAAGGGAGCA ACTCCAATCC AACAGAAATG CATCCCAGCG 
ATATTGGCGG GCCAGGATTG CATTGGAGCT GCCAAGACGG GCTCGGGCAA GACCTTCGCC 
TTTGCCCTGC CCATTCTGGA GCGGCTGAGC GAAGAGCCAG TGAGCCACTT CGCCCTGGTG 
CTGACGCCCA CGCACGAGTT GGCCTATCAA ATCTCCGAGC AGTTTCTTGT GGCTGGACAG 
GCGATGGGGG TGCGCGTATG TGTTGTCTCT GGCGGCACAG ACCAAATGGT CGAGAGCCAG 
AAGCTTATGC AGCGACCGCA CATCGTGGTG GCCATGCCCG GCCGTCTCGC GGATCACCTA 
ACCGGCTGCG ACACCTTCTC CTTCGACAAT CTGAAGTATT TGGTTGTTGA CGAAGCGGAT 
CGCATGCTGA ACGGAGACTT TGACGAGAGC CTATCCATCA TTGAGCGCTG TTTGCCAAAG 
ACAAGGCAAA ACCTTTTCTT TTCCGCAACC ATGAAGGACT TCATAAAGGA GTCCAGCATA 
TTTCCCATTG CCAGCGATTG TTTTGAATGG TCGCAGGACT CAGACGTGGC CACTGTAGAG 
ACTCTGGACC AGCGTTATCT GCTGTGCGCC GACTACGATC GCGACATGGT CTTAATAGAG 
GCACTAAGAA AGTATCGCGA GGAGAACGAA AACGCTAATG TCATGATCTT CACCAACACA 
AAAAAGTACT GTCAGCTTCT CTCGATGACA CTGAAGAACA TGGAAATCGA TAATGTTTGC 
TTGCATGGTT TCATGCGGCA AAAGGAGCGC GTAGCTGCTC TAAGTCGCTT CAAGTCCAAC 
CAAATACGTA CACTGATCGC TACGGATGTG GCCGCCAGAG GTCTGGATAT ACCCAGCGTC 
GAGCTGGTTA TGAATCACAT GCTTCCCCGG ACACCAAAGG AGTACATCCA TCGCGTAGGC 
AGAACAGCAA GAGCGGGGCG CAAGGGCATG TCCATCTCCA TATTCCGCTT TCCACGCGAT 
CTAGAGCTTT TGGCCGCCAT TGAGGAGGAG ATCAACACCA AACTTACAGA GCATCCCATC 
GATCAGCGCA TGGTGGAGAG AATCTTTATG CAAGTAAATG TCACGCGCCG TGAATCAGAG 
ATGCAATTAG ATAATAACGA TTTCGACGAG CGAGCGCAAA ACTATAGGCG AAAGACGTGG 
ATTATGGAGG GGAAGGACCC CGACCAAATG GAAGCTTTGT ATCGTAAAAA ACAGAAGGAT 
AAACTAAGGG AAATACGCCG AAAGAGAAAA CTGCAGCACG CAGAGCCGGC AGCCAGCGAA 
GAGGGCAAAG CTCTCCTACA GGACGAGCGT TTTAAATCTG TAGACAGTGC GAGGTTTGAG 
AAGAAGGGAA AAGGACGAAG TAGAGCCACC CAAGAAGATA CCCCAACCAA GCCACTGAAA 
AGGCTTAACA AAGAAAAACC AGTTGCCCAA AAGGGCAGAG CAGATGTCAA GAAAGATAAA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476927.1
CDS	78..1643
Translation	MQRKEANPFQILGLRPWLVKQLTKLGLKGATPIQQKCIPAILAGQDCIGAAKTGSGKTFAFALPILERLSEEPVSHFALVLTPTHELAYQISEQFLVAGQAMGVRVCVVSGGTDQMVESQKLMQRPHIVVAMPGRLADHLTGCDTFSFDNLKYLVVDEADRMLNGDFDESLSIIERCLPKTRQNLFFSATMKDFIKESSIFPIASDCFEWSQDSDVATVETLDQRYLLCADYDRDMVLIEALRKYREENENANVMIFTNTKKYCQLLSMTLKNMEIDNVCLHGFMRQKERVAALSRFKSNQIRTLIATDVAARGLDIPSVELVMNHMLPRTPKEYIHRVGRTARAGRKGMSISIFRFPRDLELLAAIEEEINTKLTEHPIDQRMVERIFMQVNVTRRESEMQLDNNDFDERAQNYRRKTWIMEGKDPDQMEALYRKKQKDKLREIRRKRKLQHAEPAASEEGKALLQDERFKSVDSARFEKKGKGRSRATQEDTPTKPLKRLNKEKPVAQKGRADVKKDKA

Target ORF information:

RefSeq Version	NM_057579.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DEAD box protein 45A (Dbp45A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057579.5

ORF Insert Sequence:

ATGCAACGAA AGGAGGCCAA TCCCTTTCAG ATCCTGGGCC TACGTCCATG GTTGGTTAAG 
CAGCTGACCA AGTTGGGCCT GAAGGGAGCA ACTCCAATCC AACAGAAATG CATCCCAGCG 
ATATTGGCGG GCCAGGATTG CATTGGAGCT GCCAAGACGG GCTCGGGCAA GACCTTCGCC 
TTTGCCCTGC CCATTCTGGA GCGGCTGAGC GAAGAGCCAG TGAGCCACTT CGCCCTGGTG 
CTGACGCCCA CGCACGAGTT GGCCTATCAA ATCTCCGAGC AGTTTCTTGT GGCTGGACAG 
GCGATGGGGG TGCGCGTATG TGTTGTCTCT GGCGGCACAG ACCAAATGGT CGAGAGCCAG 
AAGCTTATGC AGCGACCGCA CATCGTGGTG GCCATGCCCG GCCGTCTCGC GGATCACCTA 
ACCGGCTGCG ACACCTTCTC CTTCGACAAT CTGAAGTATT TGGTTGTTGA CGAAGCGGAT 
CGCATGCTGA ACGGAGACTT TGACGAGAGC CTATCCATCA TTGAGCGCTG TTTGCCAAAG 
ACAAGGCAAA ACCTTTTCTT TTCCGCAACC ATGAAGGACT TCATAAAGGA GTCCAGCATA 
TTTCCCATTG CCAGCGATTG TTTTGAATGG TCGCAGGACT CAGACGTGGC CACTGTAGAG 
ACTCTGGACC AGCGTTATCT GCTGTGCGCC GACTACGATC GCGACATGGT CTTAATAGAG 
GCACTAAGAA AGTATCGCGA GGAGAACGAA AACGCTAATG TCATGATCTT CACCAACACA 
AAAAAGTACT GTCAGCTTCT CTCGATGACA CTGAAGAACA TGGAAATCGA TAATGTTTGC 
TTGCATGGTT TCATGCGGCA AAAGGAGCGC GTAGCTGCTC TAAGTCGCTT CAAGTCCAAC 
CAAATACGTA CACTGATCGC TACGGATGTG GCCGCCAGAG GTCTGGATAT ACCCAGCGTC 
GAGCTGGTTA TGAATCACAT GCTTCCCCGG ACACCAAAGG AGTACATCCA TCGCGTAGGC 
AGAACAGCAA GAGCGGGGCG CAAGGGCATG TCCATCTCCA TATTCCGCTT TCCACGCGAT 
CTAGAGCTTT TGGCCGCCAT TGAGGAGGAG ATCAACACCA AACTTACAGA GCATCCCATC 
GATCAGCGCA TGGTGGAGAG AATCTTTATG CAAGTAAATG TCACGCGCCG TGAATCAGAG 
ATGCAATTAG ATAATAACGA TTTCGACGAG CGAGCGCAAA ACTATAGGCG AAAGACGTGG 
ATTATGGAGG GGAAGGACCC CGACCAAATG GAAGCTTTGT ATCGTAAAAA ACAGAAGGAT 
AAACTAAGGG AAATACGCCG AAAGAGAAAA CTGCAGCACG CAGAGCCGGC AGCCAGCGAA 
GAGGGCAAAG CTCTCCTACA GGACGAGCGT TTTAAATCTG TAGACAGTGC GAGGTTTGAG 
AAGAAGGGAA AAGGACGAAG TAGAGCCACC CAAGAAGATA CCCCAACCAA GCCACTGAAA 
AGGCTTAACA AAGAAAAACC AGTTGCCCAA AAGGGCAGAG CAGATGTCAA GAAAGATAAA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dbp45A cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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