Rat1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rat1
Entrez Gene ID	33964
Full Name	Rat1 5'-3' exoribonuclease
Synonyms	CG10354,Dmel\CG10354,RAT1,XRN2,Xrn2,dRAT1,dhp1
General protein information	Preferred Names Rat1 5'-3' exoribonuclease Names CG10354 gene product from transcript CG10354-RA CG10354-PA CG10354-PB Rat1-PA Rat1-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27C4-27C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273237.1	NP_001260166.1	Rat1 5'-3' exoribonuclease, isoform B
NM_135238.3	NP_609082.1	Rat1 5'-3' exoribonuclease, isoform A

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGR303W	NP_986969.2
Pan troglodytes (chimpanzee)	XRN2	XP_514546.2
Canis lupus familiaris (dog)	XRN2	XP_534324.2
Rattus norvegicus (Norway rat)	Xrn2	NP_001102066.1
Drosophila melanogaster (fruit fly)	Rat1	NP_001260166.1
Caenorhabditis elegans (roundworm)	xrn-2	NP_496958.2
Saccharomyces cerevisiae (baker's yeast)	RAT1	NP_014691.1
Xenopus tropicalis (tropical clawed frog)	xrn2	XP_002939479.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004090	XP_309641.5
Schizosaccharomyces pombe (fission yeast)	dhp1	NP_594155.1
Neurospora crassa	NCU01643	XP_956265.1
Homo sapiens (human)	XRN2	NP_036387.2
Bos taurus (cattle)	XRN2	NP_001179401.1
Mus musculus (house mouse)	Xrn2	NP_036047.2
Magnaporthe oryzae (rice blast fungus)	MGG_11426	XP_003717803.1
Macaca mulatta (Rhesus monkey)	XRN2	XP_001094734.2
Gallus gallus (chicken)	XRN2	NP_001026204.1
Danio rerio (zebrafish)	xrn2	NP_001001944.2
Kluyveromyces lactis	KLLA0F07469g	XP_455419.1
Arabidopsis thaliana (thale cress)	XRN3	NP_565114.1
Oryza sativa (rice)	Os01g0872700	NP_001044947.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13968	NM_001273237.1 Latest version!	Drosophila melanogaster Rat1 5'-3' exoribonuclease (Rat1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa13968	NM_135238.3 Latest version!	Drosophila melanogaster Rat1 5'-3' exoribonuclease (Rat1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rat1 ( NM_001273237.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260166.1 Drosophila melanogaster Rat1 5'-3' exoribonuclease (Rat1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13968
Clone ID Related Accession (Same CDS sequence)	NM_001273237.1 , NM_135238.3
Accession Version	NM_001273237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rat1 5'-3' exoribonuclease, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAGTTC CAGCATTCTT TCGCTGGCTG AGCCGGAAGT ATCCATCCGT GATAATAGAA 
TGCAATGAAA ACAAGCAGGT GGACGCGGAC ACCGGTCGAA ACATCTACGA GGACCCCACC 
TTGCCGAATC CAAATGGGAT AGAGTTTGAC AATCTGTACC TGGACATGAA CGGCATCATC 
CATCCGTGCA CGCATCCGGA GGACAAGCCG GCGCCGAAGA ACGAGGACGA GATGATGGTG 
GCCATCTTCG AATGCATCGA CCGTCTGTTC GGTATTGTAC GTCCTCGAAA ACTGCTCTAT 
ATGGCCATCG ATGGCGTTGC TCCACGAGCC AAGATGAATC AGCAGCGATC TCGTCGCTTC 
CGAGCGGCCA AGGAAACCAC CGAAAAGCGG CTGGAGATAG CACGGATCCG CGAGGAGCTG 
CTGAGCCGCG GCTGCAAGCT GCCACCGGAG AAGGAGAAGG GTGAACACTT TGACTCCAAC 
TGCATCACGC CGGGCACCCC GTTCATGGAC CGACTGAGCA AGTGTTTGCA CTACTTTGTT 
CATGATCGTC AAAACAATAA CCCGGCCTGG AAGGGCATCA AGGTGATCCT GTCGGACGCC 
AATGTGCCCG GTGAGGGTGA GCACAAGATC ATGGATTACA TTCGCAAGCA GCGTGCCCAG 
CCTGATCACG ATCCCAACAC GCAGCACGTC TTGTGCGGAG CCGACGCCGA TCTCATCATG 
TTGGGTCTGG CCACGCACGA GCCCAACTTC ACAATCATTC GGGAGGAGTT CCTGCCGAAT 
AAGCCGCGTC CCTGCGACAT CTGCAACGGA TTTGGCCACG AGATGGACAA GTGTGTCGGA 
CTCGGAGCGA CTGCGCCAAC TAGCGCCAAT TTTAAGCCCG ATGTGCCCAT AGGCGCCGAG 
GTAAAGTTCA TCTTTGTGCG GCTGAGTGTG CTACGCGAGT ACCTAAAGCA GACTCTTGAG 
ATGCCGAATC TGCCATTTGA GTACAGTTTC GAGCGCGCGT TGGACGACTG GGTGTTCATG 
TGCTTCTTTG TGGGCAATGA TTTTCTGCCC CACCTGCCTA GCCTGGAGAT TCGCGAGGGT 
GCAGTAGATC GATTGGTGGA GTTATACAAG AAGTGCGTTT ACAAGACAAG GGGCTATCTC 
ACCGACTCGG GAGATGTAAA TCTTGACCGG GTGCAGCTGA TTATGACCGA CTTGGGCAAT 
GCCGAGGACC AGATCTTCAA GAGCCGACAG CGACGCGAGG AGCAGTTCAA GGCCAGGGAC 
AAGGCGCGCA AGAGGCAGGA AAGGAATCAG GACCACGGAT CCTTGAATCA GTCTGCTTTT 
GGCGCCAGCG CCGTGGGTCC AAACAGCCAG CAAAGGAGCG TCGGCAACTA CAAGGAGGAG 
GCCGCGGCAC TTCGTAATAG AAAACGGACG AGTGACATGG CCAATTTGGA TGACGAAGAT 
GAGGAGGAGA ATAACGACGA GGTGCGATTG TGGGAGGATG GCTTCAAGGA CCGCTACTAC 
GAATCCAAGT TTGACGTGGC ACCGGGTAAC CAGCAGTTCC GATACGCTGT AGCCCTGCAG 
TATGTCCGAG GATTGTGCTG GGTTCTTAAG TACTACTATC AGGGCTGTGC CTCCTGGAAC 
TGGTACTTCC CATACCATTA TGCACCCTTC GCATCGGATT TTGTCAACAT CCAGGGACTG 
TCCACGATGT TCGAAAAGGG CACAAAGCCG TTTAATCCGC TCGAGCAACT GATGGGCGTC 
TTTCCGGCAG CCAGTAGTTC ACACGTGCCC GAGCCGTGGG CCAAGCTCAT GTCCGATCCG 
GAGTCACCGA TCATTGACTT CTATCCGGAG GACTTTAAGA TCGATCTGAA TGGCAAGAAG 
TTCGCCTGGC AGGGAGTGGC TCTGCTGCCG TTCGTCGATG AGAAGCGTCT CTTCAAGGCT 
TTGGTTCCCT ACTATGATCA ACTTACTGGA GAGGAGGTGA AGCGAAACAA GCGTGGAGAC 
AATTACCTGT ACATCAGCAA TCAGAGTCCG CACTACAAAA AGGTGAAGAA AATCAGCGAA 
AAGGATGATG AGAGCGTTTG CAAAGCCATC TCATTTGACG GTATGCGCGG CACTTTGGGA 
AAAACCGAAC TAAACACTGC CATCAGCGGC GTTCTTAAGT CGCCGATTTC CGGGCTATCG 
GACATTAATG ACAATATAAC CGTGACGACC ACGTTCAGGG ATCCGGAATA CGATGAAGAC 
TACATTTTCG AAGCGAAGCG CCTCGAAAAC GCCGTAGATC CGCCACAGGT GCTGCCAAAT 
GAGCAGTCTG GTAACAAGCA TCGTCCCGTC ATCGGTTTTA ATAGCCACTT GACCAGGGCC 
TACGTTCCGG ATAGTGGTCA CCGCATGCTG AACGCTGGCA TAAGGAACCA ACAGGGTGGC 
GGTGGCAATG GAGGTGGAGG AGGAGGCTAC GGGCAAGGAG GCGGCTACGG CCAGGGAATA 
GGCGGAAACC AAGGACAGAG CTATCAGAAC AATAGCCGAA ACTATAACTA CAACTACAAC 
AATAACTACA ATCAGCACCA GGGTGGCGGC TATCAAAACA ACTACAATAA CCGACAGCAG 
CAGCAATATG GCCACAATCA GAGATTCAAC CAGGACAATT CGAATCAGCA GCAGCGCAAC 
TTTAACAACT ATAATGGTCC AAGGAACAAC AACTACCAGC AACAAGGCGG CAGCAGGCAA 
CAGAATCAGA ATTATAGACG GTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260166.1
CDS	84..2810
Translation	MGVPAFFRWLSRKYPSVIIECNENKQVDADTGRNIYEDPTLPNPNGIEFDNLYLDMNGIIHPCTHPEDKPAPKNEDEMMVAIFECIDRLFGIVRPRKLLYMAIDGVAPRAKMNQQRSRRFRAAKETTEKRLEIARIREELLSRGCKLPPEKEKGEHFDSNCITPGTPFMDRLSKCLHYFVHDRQNNNPAWKGIKVILSDANVPGEGEHKIMDYIRKQRAQPDHDPNTQHVLCGADADLIMLGLATHEPNFTIIREEFLPNKPRPCDICNGFGHEMDKCVGLGATAPTSANFKPDVPIGAEVKFIFVRLSVLREYLKQTLEMPNLPFEYSFERALDDWVFMCFFVGNDFLPHLPSLEIREGAVDRLVELYKKCVYKTRGYLTDSGDVNLDRVQLIMTDLGNAEDQIFKSRQRREEQFKARDKARKRQERNQDHGSLNQSAFGASAVGPNSQQRSVGNYKEEAAALRNRKRTSDMANLDDEDEEENNDEVRLWEDGFKDRYYESKFDVAPGNQQFRYAVALQYVRGLCWVLKYYYQGCASWNWYFPYHYAPFASDFVNIQGLSTMFEKGTKPFNPLEQLMGVFPAASSSHVPEPWAKLMSDPESPIIDFYPEDFKIDLNGKKFAWQGVALLPFVDEKRLFKALVPYYDQLTGEEVKRNKRGDNYLYISNQSPHYKKVKKISEKDDESVCKAISFDGMRGTLGKTELNTAISGVLKSPISGLSDINDNITVTTTFRDPEYDEDYIFEAKRLENAVDPPQVLPNEQSGNKHRPVIGFNSHLTRAYVPDSGHRMLNAGIRNQQGGGGNGGGGGGYGQGGGYGQGIGGNQGQSYQNNSRNYNYNYNNNYNQHQGGGYQNNYNNRQQQQYGHNQRFNQDNSNQQQRNFNNYNGPRNNNYQQQGGSRQQNQNYRRF

Target ORF information:

RefSeq Version	NM_001273237.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rat1 5'-3' exoribonuclease (Rat1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273237.1

ORF Insert Sequence:

ATGGGAGTTC CAGCATTCTT TCGCTGGCTG AGCCGGAAGT ATCCATCCGT GATAATAGAA 
TGCAATGAAA ACAAGCAGGT GGACGCGGAC ACCGGTCGAA ACATCTACGA GGACCCCACC 
TTGCCGAATC CAAATGGGAT AGAGTTTGAC AATCTGTACC TGGACATGAA CGGCATCATC 
CATCCGTGCA CGCATCCGGA GGACAAGCCG GCGCCGAAGA ACGAGGACGA GATGATGGTG 
GCCATCTTCG AATGCATCGA CCGTCTGTTC GGTATTGTAC GTCCTCGAAA ACTGCTCTAT 
ATGGCCATCG ATGGCGTTGC TCCACGAGCC AAGATGAATC AGCAGCGATC TCGTCGCTTC 
CGAGCGGCCA AGGAAACCAC CGAAAAGCGG CTGGAGATAG CACGGATCCG CGAGGAGCTG 
CTGAGCCGCG GCTGCAAGCT GCCACCGGAG AAGGAGAAGG GTGAACACTT TGACTCCAAC 
TGCATCACGC CGGGCACCCC GTTCATGGAC CGACTGAGCA AGTGTTTGCA CTACTTTGTT 
CATGATCGTC AAAACAATAA CCCGGCCTGG AAGGGCATCA AGGTGATCCT GTCGGACGCC 
AATGTGCCCG GTGAGGGTGA GCACAAGATC ATGGATTACA TTCGCAAGCA GCGTGCCCAG 
CCTGATCACG ATCCCAACAC GCAGCACGTC TTGTGCGGAG CCGACGCCGA TCTCATCATG 
TTGGGTCTGG CCACGCACGA GCCCAACTTC ACAATCATTC GGGAGGAGTT CCTGCCGAAT 
AAGCCGCGTC CCTGCGACAT CTGCAACGGA TTTGGCCACG AGATGGACAA GTGTGTCGGA 
CTCGGAGCGA CTGCGCCAAC TAGCGCCAAT TTTAAGCCCG ATGTGCCCAT AGGCGCCGAG 
GTAAAGTTCA TCTTTGTGCG GCTGAGTGTG CTACGCGAGT ACCTAAAGCA GACTCTTGAG 
ATGCCGAATC TGCCATTTGA GTACAGTTTC GAGCGCGCGT TGGACGACTG GGTGTTCATG 
TGCTTCTTTG TGGGCAATGA TTTTCTGCCC CACCTGCCTA GCCTGGAGAT TCGCGAGGGT 
GCAGTAGATC GATTGGTGGA GTTATACAAG AAGTGCGTTT ACAAGACAAG GGGCTATCTC 
ACCGACTCGG GAGATGTAAA TCTTGACCGG GTGCAGCTGA TTATGACCGA CTTGGGCAAT 
GCCGAGGACC AGATCTTCAA GAGCCGACAG CGACGCGAGG AGCAGTTCAA GGCCAGGGAC 
AAGGCGCGCA AGAGGCAGGA AAGGAATCAG GACCACGGAT CCTTGAATCA GTCTGCTTTT 
GGCGCCAGCG CCGTGGGTCC AAACAGCCAG CAAAGGAGCG TCGGCAACTA CAAGGAGGAG 
GCCGCGGCAC TTCGTAATAG AAAACGGACG AGTGACATGG CCAATTTGGA TGACGAAGAT 
GAGGAGGAGA ATAACGACGA GGTGCGATTG TGGGAGGATG GCTTCAAGGA CCGCTACTAC 
GAATCCAAGT TTGACGTGGC ACCGGGTAAC CAGCAGTTCC GATACGCTGT AGCCCTGCAG 
TATGTCCGAG GATTGTGCTG GGTTCTTAAG TACTACTATC AGGGCTGTGC CTCCTGGAAC 
TGGTACTTCC CATACCATTA TGCACCCTTC GCATCGGATT TTGTCAACAT CCAGGGACTG 
TCCACGATGT TCGAAAAGGG CACAAAGCCG TTTAATCCGC TCGAGCAACT GATGGGCGTC 
TTTCCGGCAG CCAGTAGTTC ACACGTGCCC GAGCCGTGGG CCAAGCTCAT GTCCGATCCG 
GAGTCACCGA TCATTGACTT CTATCCGGAG GACTTTAAGA TCGATCTGAA TGGCAAGAAG 
TTCGCCTGGC AGGGAGTGGC TCTGCTGCCG TTCGTCGATG AGAAGCGTCT CTTCAAGGCT 
TTGGTTCCCT ACTATGATCA ACTTACTGGA GAGGAGGTGA AGCGAAACAA GCGTGGAGAC 
AATTACCTGT ACATCAGCAA TCAGAGTCCG CACTACAAAA AGGTGAAGAA AATCAGCGAA 
AAGGATGATG AGAGCGTTTG CAAAGCCATC TCATTTGACG GTATGCGCGG CACTTTGGGA 
AAAACCGAAC TAAACACTGC CATCAGCGGC GTTCTTAAGT CGCCGATTTC CGGGCTATCG 
GACATTAATG ACAATATAAC CGTGACGACC ACGTTCAGGG ATCCGGAATA CGATGAAGAC 
TACATTTTCG AAGCGAAGCG CCTCGAAAAC GCCGTAGATC CGCCACAGGT GCTGCCAAAT 
GAGCAGTCTG GTAACAAGCA TCGTCCCGTC ATCGGTTTTA ATAGCCACTT GACCAGGGCC 
TACGTTCCGG ATAGTGGTCA CCGCATGCTG AACGCTGGCA TAAGGAACCA ACAGGGTGGC 
GGTGGCAATG GAGGTGGAGG AGGAGGCTAC GGGCAAGGAG GCGGCTACGG CCAGGGAATA 
GGCGGAAACC AAGGACAGAG CTATCAGAAC AATAGCCGAA ACTATAACTA CAACTACAAC 
AATAACTACA ATCAGCACCA GGGTGGCGGC TATCAAAACA ACTACAATAA CCGACAGCAG 
CAGCAATATG GCCACAATCA GAGATTCAAC CAGGACAATT CGAATCAGCA GCAGCGCAAC 
TTTAACAACT ATAATGGTCC AAGGAACAAC AACTACCAGC AACAAGGCGG CAGCAGGCAA 
CAGAATCAGA ATTATAGACG GTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rat1 ( NM_135238.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609082.1 Drosophila melanogaster Rat1 5'-3' exoribonuclease (Rat1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13968
Clone ID Related Accession (Same CDS sequence)	NM_001273237.1 , NM_135238.3
Accession Version	NM_135238.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rat1 5'-3' exoribonuclease, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135238.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAGTTC CAGCATTCTT TCGCTGGCTG AGCCGGAAGT ATCCATCCGT GATAATAGAA 
TGCAATGAAA ACAAGCAGGT GGACGCGGAC ACCGGTCGAA ACATCTACGA GGACCCCACC 
TTGCCGAATC CAAATGGGAT AGAGTTTGAC AATCTGTACC TGGACATGAA CGGCATCATC 
CATCCGTGCA CGCATCCGGA GGACAAGCCG GCGCCGAAGA ACGAGGACGA GATGATGGTG 
GCCATCTTCG AATGCATCGA CCGTCTGTTC GGTATTGTAC GTCCTCGAAA ACTGCTCTAT 
ATGGCCATCG ATGGCGTTGC TCCACGAGCC AAGATGAATC AGCAGCGATC TCGTCGCTTC 
CGAGCGGCCA AGGAAACCAC CGAAAAGCGG CTGGAGATAG CACGGATCCG CGAGGAGCTG 
CTGAGCCGCG GCTGCAAGCT GCCACCGGAG AAGGAGAAGG GTGAACACTT TGACTCCAAC 
TGCATCACGC CGGGCACCCC GTTCATGGAC CGACTGAGCA AGTGTTTGCA CTACTTTGTT 
CATGATCGTC AAAACAATAA CCCGGCCTGG AAGGGCATCA AGGTGATCCT GTCGGACGCC 
AATGTGCCCG GTGAGGGTGA GCACAAGATC ATGGATTACA TTCGCAAGCA GCGTGCCCAG 
CCTGATCACG ATCCCAACAC GCAGCACGTC TTGTGCGGAG CCGACGCCGA TCTCATCATG 
TTGGGTCTGG CCACGCACGA GCCCAACTTC ACAATCATTC GGGAGGAGTT CCTGCCGAAT 
AAGCCGCGTC CCTGCGACAT CTGCAACGGA TTTGGCCACG AGATGGACAA GTGTGTCGGA 
CTCGGAGCGA CTGCGCCAAC TAGCGCCAAT TTTAAGCCCG ATGTGCCCAT AGGCGCCGAG 
GTAAAGTTCA TCTTTGTGCG GCTGAGTGTG CTACGCGAGT ACCTAAAGCA GACTCTTGAG 
ATGCCGAATC TGCCATTTGA GTACAGTTTC GAGCGCGCGT TGGACGACTG GGTGTTCATG 
TGCTTCTTTG TGGGCAATGA TTTTCTGCCC CACCTGCCTA GCCTGGAGAT TCGCGAGGGT 
GCAGTAGATC GATTGGTGGA GTTATACAAG AAGTGCGTTT ACAAGACAAG GGGCTATCTC 
ACCGACTCGG GAGATGTAAA TCTTGACCGG GTGCAGCTGA TTATGACCGA CTTGGGCAAT 
GCCGAGGACC AGATCTTCAA GAGCCGACAG CGACGCGAGG AGCAGTTCAA GGCCAGGGAC 
AAGGCGCGCA AGAGGCAGGA AAGGAATCAG GACCACGGAT CCTTGAATCA GTCTGCTTTT 
GGCGCCAGCG CCGTGGGTCC AAACAGCCAG CAAAGGAGCG TCGGCAACTA CAAGGAGGAG 
GCCGCGGCAC TTCGTAATAG AAAACGGACG AGTGACATGG CCAATTTGGA TGACGAAGAT 
GAGGAGGAGA ATAACGACGA GGTGCGATTG TGGGAGGATG GCTTCAAGGA CCGCTACTAC 
GAATCCAAGT TTGACGTGGC ACCGGGTAAC CAGCAGTTCC GATACGCTGT AGCCCTGCAG 
TATGTCCGAG GATTGTGCTG GGTTCTTAAG TACTACTATC AGGGCTGTGC CTCCTGGAAC 
TGGTACTTCC CATACCATTA TGCACCCTTC GCATCGGATT TTGTCAACAT CCAGGGACTG 
TCCACGATGT TCGAAAAGGG CACAAAGCCG TTTAATCCGC TCGAGCAACT GATGGGCGTC 
TTTCCGGCAG CCAGTAGTTC ACACGTGCCC GAGCCGTGGG CCAAGCTCAT GTCCGATCCG 
GAGTCACCGA TCATTGACTT CTATCCGGAG GACTTTAAGA TCGATCTGAA TGGCAAGAAG 
TTCGCCTGGC AGGGAGTGGC TCTGCTGCCG TTCGTCGATG AGAAGCGTCT CTTCAAGGCT 
TTGGTTCCCT ACTATGATCA ACTTACTGGA GAGGAGGTGA AGCGAAACAA GCGTGGAGAC 
AATTACCTGT ACATCAGCAA TCAGAGTCCG CACTACAAAA AGGTGAAGAA AATCAGCGAA 
AAGGATGATG AGAGCGTTTG CAAAGCCATC TCATTTGACG GTATGCGCGG CACTTTGGGA 
AAAACCGAAC TAAACACTGC CATCAGCGGC GTTCTTAAGT CGCCGATTTC CGGGCTATCG 
GACATTAATG ACAATATAAC CGTGACGACC ACGTTCAGGG ATCCGGAATA CGATGAAGAC 
TACATTTTCG AAGCGAAGCG CCTCGAAAAC GCCGTAGATC CGCCACAGGT GCTGCCAAAT 
GAGCAGTCTG GTAACAAGCA TCGTCCCGTC ATCGGTTTTA ATAGCCACTT GACCAGGGCC 
TACGTTCCGG ATAGTGGTCA CCGCATGCTG AACGCTGGCA TAAGGAACCA ACAGGGTGGC 
GGTGGCAATG GAGGTGGAGG AGGAGGCTAC GGGCAAGGAG GCGGCTACGG CCAGGGAATA 
GGCGGAAACC AAGGACAGAG CTATCAGAAC AATAGCCGAA ACTATAACTA CAACTACAAC 
AATAACTACA ATCAGCACCA GGGTGGCGGC TATCAAAACA ACTACAATAA CCGACAGCAG 
CAGCAATATG GCCACAATCA GAGATTCAAC CAGGACAATT CGAATCAGCA GCAGCGCAAC 
TTTAACAACT ATAATGGTCC AAGGAACAAC AACTACCAGC AACAAGGCGG CAGCAGGCAA 
CAGAATCAGA ATTATAGACG GTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609082.1
CDS	84..2810
Translation	MGVPAFFRWLSRKYPSVIIECNENKQVDADTGRNIYEDPTLPNPNGIEFDNLYLDMNGIIHPCTHPEDKPAPKNEDEMMVAIFECIDRLFGIVRPRKLLYMAIDGVAPRAKMNQQRSRRFRAAKETTEKRLEIARIREELLSRGCKLPPEKEKGEHFDSNCITPGTPFMDRLSKCLHYFVHDRQNNNPAWKGIKVILSDANVPGEGEHKIMDYIRKQRAQPDHDPNTQHVLCGADADLIMLGLATHEPNFTIIREEFLPNKPRPCDICNGFGHEMDKCVGLGATAPTSANFKPDVPIGAEVKFIFVRLSVLREYLKQTLEMPNLPFEYSFERALDDWVFMCFFVGNDFLPHLPSLEIREGAVDRLVELYKKCVYKTRGYLTDSGDVNLDRVQLIMTDLGNAEDQIFKSRQRREEQFKARDKARKRQERNQDHGSLNQSAFGASAVGPNSQQRSVGNYKEEAAALRNRKRTSDMANLDDEDEEENNDEVRLWEDGFKDRYYESKFDVAPGNQQFRYAVALQYVRGLCWVLKYYYQGCASWNWYFPYHYAPFASDFVNIQGLSTMFEKGTKPFNPLEQLMGVFPAASSSHVPEPWAKLMSDPESPIIDFYPEDFKIDLNGKKFAWQGVALLPFVDEKRLFKALVPYYDQLTGEEVKRNKRGDNYLYISNQSPHYKKVKKISEKDDESVCKAISFDGMRGTLGKTELNTAISGVLKSPISGLSDINDNITVTTTFRDPEYDEDYIFEAKRLENAVDPPQVLPNEQSGNKHRPVIGFNSHLTRAYVPDSGHRMLNAGIRNQQGGGGNGGGGGGYGQGGGYGQGIGGNQGQSYQNNSRNYNYNYNNNYNQHQGGGYQNNYNNRQQQQYGHNQRFNQDNSNQQQRNFNNYNGPRNNNYQQQGGSRQQNQNYRRF

Target ORF information:

RefSeq Version	NM_135238.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rat1 5'-3' exoribonuclease (Rat1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135238.3

ORF Insert Sequence:

ATGGGAGTTC CAGCATTCTT TCGCTGGCTG AGCCGGAAGT ATCCATCCGT GATAATAGAA 
TGCAATGAAA ACAAGCAGGT GGACGCGGAC ACCGGTCGAA ACATCTACGA GGACCCCACC 
TTGCCGAATC CAAATGGGAT AGAGTTTGAC AATCTGTACC TGGACATGAA CGGCATCATC 
CATCCGTGCA CGCATCCGGA GGACAAGCCG GCGCCGAAGA ACGAGGACGA GATGATGGTG 
GCCATCTTCG AATGCATCGA CCGTCTGTTC GGTATTGTAC GTCCTCGAAA ACTGCTCTAT 
ATGGCCATCG ATGGCGTTGC TCCACGAGCC AAGATGAATC AGCAGCGATC TCGTCGCTTC 
CGAGCGGCCA AGGAAACCAC CGAAAAGCGG CTGGAGATAG CACGGATCCG CGAGGAGCTG 
CTGAGCCGCG GCTGCAAGCT GCCACCGGAG AAGGAGAAGG GTGAACACTT TGACTCCAAC 
TGCATCACGC CGGGCACCCC GTTCATGGAC CGACTGAGCA AGTGTTTGCA CTACTTTGTT 
CATGATCGTC AAAACAATAA CCCGGCCTGG AAGGGCATCA AGGTGATCCT GTCGGACGCC 
AATGTGCCCG GTGAGGGTGA GCACAAGATC ATGGATTACA TTCGCAAGCA GCGTGCCCAG 
CCTGATCACG ATCCCAACAC GCAGCACGTC TTGTGCGGAG CCGACGCCGA TCTCATCATG 
TTGGGTCTGG CCACGCACGA GCCCAACTTC ACAATCATTC GGGAGGAGTT CCTGCCGAAT 
AAGCCGCGTC CCTGCGACAT CTGCAACGGA TTTGGCCACG AGATGGACAA GTGTGTCGGA 
CTCGGAGCGA CTGCGCCAAC TAGCGCCAAT TTTAAGCCCG ATGTGCCCAT AGGCGCCGAG 
GTAAAGTTCA TCTTTGTGCG GCTGAGTGTG CTACGCGAGT ACCTAAAGCA GACTCTTGAG 
ATGCCGAATC TGCCATTTGA GTACAGTTTC GAGCGCGCGT TGGACGACTG GGTGTTCATG 
TGCTTCTTTG TGGGCAATGA TTTTCTGCCC CACCTGCCTA GCCTGGAGAT TCGCGAGGGT 
GCAGTAGATC GATTGGTGGA GTTATACAAG AAGTGCGTTT ACAAGACAAG GGGCTATCTC 
ACCGACTCGG GAGATGTAAA TCTTGACCGG GTGCAGCTGA TTATGACCGA CTTGGGCAAT 
GCCGAGGACC AGATCTTCAA GAGCCGACAG CGACGCGAGG AGCAGTTCAA GGCCAGGGAC 
AAGGCGCGCA AGAGGCAGGA AAGGAATCAG GACCACGGAT CCTTGAATCA GTCTGCTTTT 
GGCGCCAGCG CCGTGGGTCC AAACAGCCAG CAAAGGAGCG TCGGCAACTA CAAGGAGGAG 
GCCGCGGCAC TTCGTAATAG AAAACGGACG AGTGACATGG CCAATTTGGA TGACGAAGAT 
GAGGAGGAGA ATAACGACGA GGTGCGATTG TGGGAGGATG GCTTCAAGGA CCGCTACTAC 
GAATCCAAGT TTGACGTGGC ACCGGGTAAC CAGCAGTTCC GATACGCTGT AGCCCTGCAG 
TATGTCCGAG GATTGTGCTG GGTTCTTAAG TACTACTATC AGGGCTGTGC CTCCTGGAAC 
TGGTACTTCC CATACCATTA TGCACCCTTC GCATCGGATT TTGTCAACAT CCAGGGACTG 
TCCACGATGT TCGAAAAGGG CACAAAGCCG TTTAATCCGC TCGAGCAACT GATGGGCGTC 
TTTCCGGCAG CCAGTAGTTC ACACGTGCCC GAGCCGTGGG CCAAGCTCAT GTCCGATCCG 
GAGTCACCGA TCATTGACTT CTATCCGGAG GACTTTAAGA TCGATCTGAA TGGCAAGAAG 
TTCGCCTGGC AGGGAGTGGC TCTGCTGCCG TTCGTCGATG AGAAGCGTCT CTTCAAGGCT 
TTGGTTCCCT ACTATGATCA ACTTACTGGA GAGGAGGTGA AGCGAAACAA GCGTGGAGAC 
AATTACCTGT ACATCAGCAA TCAGAGTCCG CACTACAAAA AGGTGAAGAA AATCAGCGAA 
AAGGATGATG AGAGCGTTTG CAAAGCCATC TCATTTGACG GTATGCGCGG CACTTTGGGA 
AAAACCGAAC TAAACACTGC CATCAGCGGC GTTCTTAAGT CGCCGATTTC CGGGCTATCG 
GACATTAATG ACAATATAAC CGTGACGACC ACGTTCAGGG ATCCGGAATA CGATGAAGAC 
TACATTTTCG AAGCGAAGCG CCTCGAAAAC GCCGTAGATC CGCCACAGGT GCTGCCAAAT 
GAGCAGTCTG GTAACAAGCA TCGTCCCGTC ATCGGTTTTA ATAGCCACTT GACCAGGGCC 
TACGTTCCGG ATAGTGGTCA CCGCATGCTG AACGCTGGCA TAAGGAACCA ACAGGGTGGC 
GGTGGCAATG GAGGTGGAGG AGGAGGCTAC GGGCAAGGAG GCGGCTACGG CCAGGGAATA 
GGCGGAAACC AAGGACAGAG CTATCAGAAC AATAGCCGAA ACTATAACTA CAACTACAAC 
AATAACTACA ATCAGCACCA GGGTGGCGGC TATCAAAACA ACTACAATAA CCGACAGCAG 
CAGCAATATG GCCACAATCA GAGATTCAAC CAGGACAATT CGAATCAGCA GCAGCGCAAC 
TTTAACAACT ATAATGGTCC AAGGAACAAC AACTACCAGC AACAAGGCGG CAGCAGGCAA 
CAGAATCAGA ATTATAGACG GTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rat1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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