cx35.4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cx35.4
Entrez Gene ID	550380
Full Name	connexin 35.4
Synonyms	gjb3,wu:fb02c04,zgc:112004
General protein information	Preferred Names connexin 35.4 Names gap junction beta-3 protein gap junction protein, beta 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017685.1	NP_001017685.1	gap junction beta-3 protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GJB3	XP_005629030.1
Danio rerio (zebrafish)	cx35.4	NP_001017685.1
Mus musculus (house mouse)	Gjb3	NP_001153484.1
Xenopus tropicalis (tropical clawed frog)	gjb3	NP_001106371.1
Homo sapiens (human)	GJB3	NP_001005752.1
Gallus gallus (chicken)	GJB3	XP_004947838.1
Macaca mulatta (Rhesus monkey)	GJB3	XP_001108150.1
Bos taurus (cattle)	GJB3	NP_001098465.1
Rattus norvegicus (Norway rat)	Gjb3	NP_062113.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

The connexin 30.3 of zebrafish homologue of human connexin 26 may play similar role in the inner ear.
Chang-Chien J, Yen YC, Chien KH, Li SY, Hsu TC, Yang JJ
Hearing research31355-66(2014 Jul)

Cx23, a connexin with only four extracellular-loop cysteines, forms functional gap junction channels and hemichannels.
Iovine MK, Gumpert AM, Falk MM, Mendelson TC
FEBS letters582(2)165-70(2008 Jan)

Evolutionary selection pressure and family relationships among connexin genes.
Cruciani V, Mikalsen SO
Biological chemistry388(3)253-64(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cx35.4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cx35.4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34929	NM_001017685.1 Latest version!	Danio rerio connexin 35.4 (cx35.4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cx35.4 ( NM_001017685.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017685.1 Danio rerio connexin 35.4 (cx35.4), mRNA.

Price & Availability↑

CloneID	ODa34929
Clone ID Related Accession (Same CDS sequence)	NM_001017685.1
Accession Version	NM_001017685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1520352000000
Organism	Danio rerio(zebrafish)
Product	gap junction beta-3 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC093154.1. ##Evidence-Data-START## Transcript exon combination :: BC093154.1, EH584273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACTGGA AGACTTTTCA AGCCCTGCTC AGCGGGGTGA ACAAATACTC CACTGCATTC 
GGCCGGATAT GGCTCTCAGT GGTTTTTGTG TTCAGGGTCA TGGTTTATGT CGTAGCGGCA 
GAAAGAGTTT GGGGTGATGA GCAGAAAGAC TTTGACTGCA ACACCAAGCA GCCGGGCTGC 
GCAAACGTCT GCTATGATTT CTACTTCCCC ATTTCCCACA TAAGACTATG GGCCCTGCAG 
CTCATCCTCG TCACGTGTCC ATCACTAATG GTGGTCATGC ACGTGAAATA CCGTGAGGAA 
CGTGAACGCA AAGCCAAAGC TAAACTCTAC GACAACACGG GAAAGAAGCA CGGTGGACTG 
TGGTGGACGT ATCTGATCAG CCTTTTTGCT AAGACTGGCA TTGAGATCAC TTTCCTGTAC 
ATCCTCCACC ACATCTATGA CAGCTTTTAC CTGCCAAGGC TGGTGAAGTG TGATGTCCAG 
CCCTGTCCCA ATGTTGTGGA CTGTTACATT GGCCGGCCCA CAGAGAAAAG AGTCTTCACT 
TATTTCATGG TGGGAGCGTC AGCGCTCTGC ATAGTGCTCA GTGTCTGCGA GATCATCTAT 
CTGATCGCCA AACGAATCAG CCGCTGCGCA AACAAATACA AGCGGCATGA CAAAAGAAGC 
ACACCGATTA ATCAGCGATA TCGAGATGAG GACAGCAACT GCACTATTCC TTTGCACGAG 
CTGGAGAGCA AGCCCGAGTA TAAACCAGAG ACTAAAGAAG ACTTTAGGTC TGAATATAAA 
CCTGGCCCTA AACCGCAGTT TAAATCTGAA GTCAAGCCCA CTTTTAAGCC AGCCTACAGG 
TTGAGTGTGG ACATGAGAGC GTCTGCTCCA AATCTCTCAG CACCAATGTA CAAAATACAG 
TCTGGTATCA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017685.1
CDS	133..1047
Translation	MDWKTFQALLSGVNKYSTAFGRIWLSVVFVFRVMVYVVAAERVWGDEQKDFDCNTKQPGCANVCYDFYFPISHIRLWALQLILVTCPSLMVVMHVKYREERERKAKAKLYDNTGKKHGGLWWTYLISLFAKTGIEITFLYILHHIYDSFYLPRLVKCDVQPCPNVVDCYIGRPTEKRVFTYFMVGASALCIVLSVCEIIYLIAKRISRCANKYKRHDKRSTPINQRYRDEDSNCTIPLHELESKPEYKPETKEDFRSEYKPGPKPQFKSEVKPTFKPAYRLSVDMRASAPNLSAPMYKIQSGII

Target ORF information:

RefSeq Version	NM_001017685.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio connexin 35.4 (cx35.4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017685.1

ORF Insert Sequence:

ATGGACTGGA AGACTTTTCA AGCCCTGCTC AGCGGGGTGA ACAAATACTC CACTGCATTC 
GGCCGGATAT GGCTCTCAGT GGTTTTTGTG TTCAGGGTCA TGGTTTATGT CGTAGCGGCA 
GAAAGAGTTT GGGGTGATGA GCAGAAAGAC TTTGACTGCA ACACCAAGCA GCCGGGCTGC 
GCAAACGTCT GCTATGATTT CTACTTCCCC ATTTCCCACA TAAGACTATG GGCCCTGCAG 
CTCATCCTCG TCACGTGTCC ATCACTAATG GTGGTCATGC ACGTGAAATA CCGTGAGGAA 
CGTGAACGCA AAGCCAAAGC TAAACTCTAC GACAACACGG GAAAGAAGCA CGGTGGACTG 
TGGTGGACGT ATCTGATCAG CCTTTTTGCT AAGACTGGCA TTGAGATCAC TTTCCTGTAC 
ATCCTCCACC ACATCTATGA CAGCTTTTAC CTGCCAAGGC TGGTGAAGTG TGATGTCCAG 
CCCTGTCCCA ATGTTGTGGA CTGTTACATT GGCCGGCCCA CAGAGAAAAG AGTCTTCACT 
TATTTCATGG TGGGAGCGTC AGCGCTCTGC ATAGTGCTCA GTGTCTGCGA GATCATCTAT 
CTGATCGCCA AACGAATCAG CCGCTGCGCA AACAAATACA AGCGGCATGA CAAAAGAAGC 
ACACCGATTA ATCAGCGATA TCGAGATGAG GACAGCAACT GCACTATTCC TTTGCACGAG 
CTGGAGAGCA AGCCCGAGTA TAAACCAGAG ACTAAAGAAG ACTTTAGGTC TGAATATAAA 
CCTGGCCCTA AACCGCAGTT TAAATCTGAA GTCAAGCCCA CTTTTAAGCC AGCCTACAGG 
TTGAGTGTGG ACATGAGAGC GTCTGCTCCA AATCTCTCAG CACCAATGTA CAAAATACAG 
TCTGGTATCA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

The connexin 30.3 of zebrafish homologue of human connexin 26 may play similar role in the inner ear.
Hearing research31355-66(2014 Jul)
Chang-Chien J,Yen YC,Chien KH,Li SY,Hsu TC,Yang JJ

Cx23, a connexin with only four extracellular-loop cysteines, forms functional gap junction channels and hemichannels.
FEBS letters582(2)165-70(2008 Jan)
Iovine MK,Gumpert AM,Falk MM,Mendelson TC

Evolutionary selection pressure and family relationships among connexin genes.
Biological chemistry388(3)253-64(2007 Mar)
Cruciani V,Mikalsen SO