emp2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	emp2
Entrez Gene ID	445506
Full Name	epithelial membrane protein 2
Synonyms	EMP-2,zgc:100935
General protein information	Preferred Names epithelial membrane protein 2 Names epithelial membrane protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017354017.1	XP_017209506.1	epithelial membrane protein 2 isoform X1
XM_021470381.1	XP_021326056.1	epithelial membrane protein 2 isoform X1
NM_001004119.1	NP_001004119.1	epithelial membrane protein 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	EMP2	NP_001415.1
Canis lupus familiaris (dog)	EMP2	XP_005621610.1
Mus musculus (house mouse)	Emp2	NP_031955.2
Pan troglodytes (chimpanzee)	EMP2	NP_001098709.1
Gallus gallus (chicken)	EMP2	XP_414935.1
Xenopus tropicalis (tropical clawed frog)	emp2	NP_001025557.1
Macaca mulatta (Rhesus monkey)	EMP2	XP_002802441.1
Rattus norvegicus (Norway rat)	Emp2	NP_001007722.1
Danio rerio (zebrafish)	emp2	NP_001004119.1
Bos taurus (cattle)	EMP2	NP_001068792.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Loss of Epithelial Membrane Protein 2 Aggravates Podocyte Injury via Upregulation of Caveolin-1.
Wan X, Chen Z, Choi WI, Gee HY, Hildebrandt F, Zhou W
Journal of the American Society of Nephrology : JASN27(4)1066-75(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutations in EMP2 cause childhood-onset nephrotic syndrome.
Gee HY, Ashraf S, Wan X, Vega-Warner V, Esteve-Rudd J, Lovric S, Fang H, Hurd TW, Sadowski CE, Allen SJ, Otto EA, Korkmaz E, Washburn J, Levy S, Williams DS, Bakkaloglu SA, Zolotnitskaya A, Ozaltin F, Zhou W, Hildebrandt F
American journal of human genetics94(6)884-90(2014 Jun)

A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier.
Ebarasi L, He L, Hultenby K, Takemoto M, Betsholtz C, Tryggvason K, Majumdar A
Developmental biology334(1)1-9(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following emp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33458	NM_001004119.1 Latest version!	Danio rerio epithelial membrane protein 2 (emp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa08347	XM_021470381.1 Latest version!	Danio rerio epithelial membrane protein 2 (emp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa08347	XM_017354017.1 Latest version!	Danio rerio epithelial membrane protein 2 (emp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

emp2 ( NM_001004119.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004119.1 Danio rerio epithelial membrane protein 2 (emp2), mRNA.

Price & Availability↑

CloneID	ODa33458
Clone ID Related Accession (Same CDS sequence)	NM_001004119.1
Accession Version	NM_001004119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	epithelial membrane protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC080211.1. ##Evidence-Data-START## Transcript exon combination :: BC080211.1, CT666231.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTGGTTA TTTTGGCCTT CATCATCCTC TTCCATATTA CCTCAGCAAT ACTACTCTTC 
ATAGCAACCA TCAATAATGC ATGGAGGATT AAAGGCGACT TCTCTATGGA TCTCTGGTAT 
AACTGCAACA GCACAGCCTG CTATGATATT CCCAAAAGTG CAACTTATGA CGCAGCATAT 
CTTCAGGCAG TGCAGGCCAC CATGATACTG GCCACCATCT TGTGCTGTGT GGGATTCTTT 
GTTTTCATTC TTCAGCTCTT CCGTCTAAAG CAGGGAGAGA GATTCGTCTT CACGGCCATA 
ATTCAGCTTC TGTCTGCTTT CTGTGTGATG ACCGGTGCTT CTATCTACAC AGCAGAGGGC 
TTAACCTTCA ATGGCCAGGA GTTTAAGAAT GCAGAGTATG GCTACTCCTT CGTCGTGGCC 
TGGGTCGCGT TCCCAATGAC GCTGCTGAGT GGCCTGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004119.1
CDS	163..624
Translation	MLVILAFIILFHITSAILLFIATINNAWRIKGDFSMDLWYNCNSTACYDIPKSATYDAAYLQAVQATMILATILCCVGFFVFILQLFRLKQGERFVFTAIIQLLSAFCVMTGASIYTAEGLTFNGQEFKNAEYGYSFVVAWVAFPMTLLSGLM

Target ORF information:

RefSeq Version	NM_001004119.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio epithelial membrane protein 2 (emp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004119.1

ORF Insert Sequence:

ATGTTGGTTA TTTTGGCCTT CATCATCCTC TTCCATATTA CCTCAGCAAT ACTACTCTTC 
ATAGCAACCA TCAATAATGC ATGGAGGATT AAAGGCGACT TCTCTATGGA TCTCTGGTAT 
AACTGCAACA GCACAGCCTG CTATGATATT CCCAAAAGTG CAACTTATGA CGCAGCATAT 
CTTCAGGCAG TGCAGGCCAC CATGATACTG GCCACCATCT TGTGCTGTGT GGGATTCTTT 
GTTTTCATTC TTCAGCTCTT CCGTCTAAAG CAGGGAGAGA GATTCGTCTT CACGGCCATA 
ATTCAGCTTC TGTCTGCTTT CTGTGTGATG ACCGGTGCTT CTATCTACAC AGCAGAGGGC 
TTAACCTTCA ATGGCCAGGA GTTTAAGAAT GCAGAGTATG GCTACTCCTT CGTCGTGGCC 
TGGGTCGCGT TCCCAATGAC GCTGCTGAGT GGCCTGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emp2 ( XM_017354017.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209506.1 Danio rerio epithelial membrane protein 2 (emp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08347
Clone ID Related Accession (Same CDS sequence)	XM_017354017.1 , XM_021470381.1
Accession Version	XM_017354017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	epithelial membrane protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTTA TTTTGGCCTT CATCATCCTC TTCCATATCA CCTCAGCAAT ACTACTCTTC 
ATAGCAACCA TCAATAATGC ATGGAGGATT AAAGGCGACT TCTCTATGGA TCTCTGGTAT 
AACTGCAACA CCACAGCCTG CTATGATATT CCCAAAAGTG CAACTTATGA CGCAGCATAT 
CTTCAGGCAG TGCAGGCCAC CATGATACTG GCCACCATCT TGTGCTGTGT GGGATTCTTT 
GTTTTCATTC TTCAGCTCTT CCGTCTAAAG CAGGGAGAGA GATTCGTCTT CACGGCCATA 
ATTCAGCTTC TGTCTGCTTT CTGTGTGATG ACCGGTGCTT CTATCTACAC AGCAGAGGGC 
TTAACCTTCA ATGGCCAGGA GTTTAAGAAT GCAGAGTATG GCTACTCCTT CGTTGTGGCC 
TGGGTCGCGT TCCCAATGAC GCTGCTGAGT GGCCTGATGT ATCTAGTGCT GAGAAAACGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209506.1
CDS	174..659
Translation	MLVILAFIILFHITSAILLFIATINNAWRIKGDFSMDLWYNCNTTACYDIPKSATYDAAYLQAVQATMILATILCCVGFFVFILQLFRLKQGERFVFTAIIQLLSAFCVMTGASIYTAEGLTFNGQEFKNAEYGYSFVVAWVAFPMTLLSGLMYLVLRKRK

Target ORF information:

RefSeq Version	XM_017354017.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio epithelial membrane protein 2 (emp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354017.1

ORF Insert Sequence:

ATGTTGGTTA TTTTGGCCTT CATCATCCTC TTCCATATCA CCTCAGCAAT ACTACTCTTC 
ATAGCAACCA TCAATAATGC ATGGAGGATT AAAGGCGACT TCTCTATGGA TCTCTGGTAT 
AACTGCAACA CCACAGCCTG CTATGATATT CCCAAAAGTG CAACTTATGA CGCAGCATAT 
CTTCAGGCAG TGCAGGCCAC CATGATACTG GCCACCATCT TGTGCTGTGT GGGATTCTTT 
GTTTTCATTC TTCAGCTCTT CCGTCTAAAG CAGGGAGAGA GATTCGTCTT CACGGCCATA 
ATTCAGCTTC TGTCTGCTTT CTGTGTGATG ACCGGTGCTT CTATCTACAC AGCAGAGGGC 
TTAACCTTCA ATGGCCAGGA GTTTAAGAAT GCAGAGTATG GCTACTCCTT CGTTGTGGCC 
TGGGTCGCGT TCCCAATGAC GCTGCTGAGT GGCCTGATGT ATCTAGTGCT GAGAAAACGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emp2 ( XM_021470381.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326056.1 Danio rerio epithelial membrane protein 2 (emp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08347
Clone ID Related Accession (Same CDS sequence)	XM_017354017.1 , XM_021470381.1
Accession Version	XM_021470381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	epithelial membrane protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTTA TTTTGGCCTT CATCATCCTC TTCCATATCA CCTCAGCAAT ACTACTCTTC 
ATAGCAACCA TCAATAATGC ATGGAGGATT AAAGGCGACT TCTCTATGGA TCTCTGGTAT 
AACTGCAACA CCACAGCCTG CTATGATATT CCCAAAAGTG CAACTTATGA CGCAGCATAT 
CTTCAGGCAG TGCAGGCCAC CATGATACTG GCCACCATCT TGTGCTGTGT GGGATTCTTT 
GTTTTCATTC TTCAGCTCTT CCGTCTAAAG CAGGGAGAGA GATTCGTCTT CACGGCCATA 
ATTCAGCTTC TGTCTGCTTT CTGTGTGATG ACCGGTGCTT CTATCTACAC AGCAGAGGGC 
TTAACCTTCA ATGGCCAGGA GTTTAAGAAT GCAGAGTATG GCTACTCCTT CGTTGTGGCC 
TGGGTCGCGT TCCCAATGAC GCTGCTGAGT GGCCTGATGT ATCTAGTGCT GAGAAAACGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326056.1
CDS	189..674
Translation	MLVILAFIILFHITSAILLFIATINNAWRIKGDFSMDLWYNCNTTACYDIPKSATYDAAYLQAVQATMILATILCCVGFFVFILQLFRLKQGERFVFTAIIQLLSAFCVMTGASIYTAEGLTFNGQEFKNAEYGYSFVVAWVAFPMTLLSGLMYLVLRKRK

Target ORF information:

RefSeq Version	XM_021470381.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio epithelial membrane protein 2 (emp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470381.1

ORF Insert Sequence:

ATGTTGGTTA TTTTGGCCTT CATCATCCTC TTCCATATCA CCTCAGCAAT ACTACTCTTC 
ATAGCAACCA TCAATAATGC ATGGAGGATT AAAGGCGACT TCTCTATGGA TCTCTGGTAT 
AACTGCAACA CCACAGCCTG CTATGATATT CCCAAAAGTG CAACTTATGA CGCAGCATAT 
CTTCAGGCAG TGCAGGCCAC CATGATACTG GCCACCATCT TGTGCTGTGT GGGATTCTTT 
GTTTTCATTC TTCAGCTCTT CCGTCTAAAG CAGGGAGAGA GATTCGTCTT CACGGCCATA 
ATTCAGCTTC TGTCTGCTTT CTGTGTGATG ACCGGTGCTT CTATCTACAC AGCAGAGGGC 
TTAACCTTCA ATGGCCAGGA GTTTAAGAAT GCAGAGTATG GCTACTCCTT CGTTGTGGCC 
TGGGTCGCGT TCCCAATGAC GCTGCTGAGT GGCCTGATGT ATCTAGTGCT GAGAAAACGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Loss of Epithelial Membrane Protein 2 Aggravates Podocyte Injury via Upregulation of Caveolin-1.
Journal of the American Society of Nephrology : JASN27(4)1066-75(2016 Apr)
Wan X,Chen Z,Choi WI,Gee HY,Hildebrandt F,Zhou W

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutations in EMP2 cause childhood-onset nephrotic syndrome.
American journal of human genetics94(6)884-90(2014 Jun)
Gee HY,Ashraf S,Wan X,Vega-Warner V,Esteve-Rudd J,Lovric S,Fang H,Hurd TW,Sadowski CE,Allen SJ,Otto EA,Korkmaz E,Washburn J,Levy S,Williams DS,Bakkaloglu SA,Zolotnitskaya A,Ozaltin F,Zhou W,Hildebrandt F

A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier.
Developmental biology334(1)1-9(2009 Oct)
Ebarasi L,He L,Hultenby K,Takemoto M,Betsholtz C,Tryggvason K,Majumdar A

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emp2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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