BRAFLDRAFT_111136 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_111136
Entrez Gene ID	7244595
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002585732.1	XP_002585778.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_111136 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_111136 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb16835	XM_002585732.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_111136 ( XM_002585732.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002585778.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb16835
Clone ID Related Accession (Same CDS sequence)	XM_002585732.1
Accession Version	XM_002585732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101229). COMPLETENESS: incomplete on both ends.

ATGTCAGGCA ATATCAAAAG TGTGATGAAC GTTAAGGCAA GTAAAGTAAA CCTAAACCCC 
AGCGTTTTGC TAAGAAAGCT CTCAGATGAG GAAGTGTTGA GAAATACAAC GCCAGAGAAA 
TTTCACAAAA CAGGAAAGCA GAGGGCAAGT GTTAGTGCCG ATACGGAAAA AAGCTTCGAA 
GTGCGACAGT CAAAGAGTGA TAGTGAGAGA AAGCAGGACT CTCAAAAGAA GGTTGAAGTA 
ACCAAGCCAC TGTCAAAAAG CACTGAGCAA AATCCTAGTC AAGCAAAACC CAGTACAGCA 
ACAGAAGTAC CACAAAAGAA AGTAGCAAAG CCAGCCAGTG AGGCTAAAGC ACAGCACAAA 
CCAACAGAAG GTAGTGTAAT AGAATCAGTG AAAGACGTGG TTGGTAAAGA GACTTCCGCA 
CCCTCCCGTA GTACAGTAAC AGAAGACCTG CTGACTCAGA TCAGTCTATC AGCCCCAGTA 
AAGGAAGTAA AGAAAGTCGT CCAAGTCCTG ACTTTGGATG ACATCCCAGG CAAAAGTGAC 
AAATCAGAAG GAAAAAGGTC TCAACTGATT GAAAAGGATA GTCAGAAAGT GTCAGCTCCA 
AAAGAAATGA CGACCATGAT GACAGAAAGT CGCAATCTGA GTGTTGTGGT ACCAAAGCTA 
CCGATGCACA TCAGTCCTCA GAAGTCATAC AACATCAATG ACCCAAGCAT CACCCCAAGC 
AAAGCCAAGC CCAGGACCCC AACAGCAAAG GAAGCACTCG CTCGGAAGAT GGTGAAGAAG 
GCAAAGACTC GAGCAAACGG GAAGGGAGCA GCGCTAAATT TCAAGCGTCT CATCGGAGAG 
AATGGAAATT TGGACAACAG CAGTGACTTT GTGGAAACAG GGAAAACTTT TCAGCCCGCT 
GTACGCAAGT TACGCAGTCG CGCAGTGAAG AAGGCTGCCA AAGATAAAGT ACGCAAACCC 
GCGAGTCCTG ACACAACAGT TTCTACAGAG AAGGGCCCTG GCAAGACTAG CAGTGTGCCA 
GTTAATGTAG AAAACAAGGA GGGGAAAACC TGTCTAACAT GCAAACAGAC CATATTTGGT 
ACAGAAGAGG CAGTGAAGGA ACACATGAAG ACTTGTTTGA GACAGCGGTT TGTGTCAGAC 
AAACATTCCT CAAGACATAC TGGTCTAAGA CACCTGCCTG AACCTGCAGA AGAGGGCCTA 
ATGGACTTAG AGGTGTCTGA TGATGAGAGT TTTGCCCGCC ACTTACAGCA GAGGGAGGAA 
GACAAATTGA TGGAAGAGCA GCTTACAGAT GCAGAGTTCT ACTTCTGCCA GATCTGTGGA 
CGTGATCTGA CTCATCTCAA CACTGTCCGG AGAACGCAGC ACATCAACCG CTGTATTGAC 
GCCATGGAGA CGGAGAAAAA ACTGCACGCA GAGCAGAACA TTAAAGATGT TCCTGACTGT 
CCCATCTGTG GCAAAGTGCT GGAAAGCAGG GCACAGAGAC AGTCCCACCT GAAGCGCTGT 
GCTGTACAGT ACGGGGTGAC CACGGCACAG CTGCTGGAAC TGGTGAAAAA GCAAGAGGAA 
GAGAAAGAGG CTCGCAGAAA GAACCAACCC AGGTCTCAAC TACAGCCAGC TCAACAGCAG 
CAAAGGGAAA TGGATGAACG GACCCAGGTT GCCATGGCAA TGTCTATGTC ACTTGTGGAG 
AAGGAAGGAT CCAGAGAGCA GCCAACAGTC ACGTCTGCAC TCCCTGTAGG TGGGAAGGAA 
AGCAAGAGAA GGAAGAAGGC TGCAAAGACT GATGTAGTAC CAGCTCTGTT GGTGACAAGT 
AAAGAGGATA GAGAAAAAAC TCTGGCTCGA AAAGTGGCCT CTATCATTGT GCAGGGTGAC 
CAGGAATCTC ACCCAAAGAC TCCAAGCCTG AGAAAAAGCA AACTGGGTAG GAAGTACCAC 
ACAGCTAGGA AGACCATGGG TAAGGGCCCA TCCTCCCCCA GCTTCCTACA GAAAGTTGGT 
GCAGCTATGA CCAGCAGCCC CCCTGCAGCT TCCACCAGTA CTGCAGCATC TCCTGCCTCC 
AGCCAGGAGA AGGCACCCCT GTGGTCCATG ACGAAGCGTA TTGACGAGAA GAAGACTGAT 
GACTTCTATG TTCAGGAGTT GTCCAAGCAG ATCACTCCAG CTAAGTATGT ACCCGTCACA 
CCAATGAGGA AGGCAGCCAA GAAAGCAATG CTGACGTTTA CGAAGAAAAA TGACACAAGC 
GAGGATGAAA CAACTGACGG CCCCCCACAG AAGGCTGTTG CGGGCAGCCA AAAGGCTAAG 
AAAACTCCCA CTGCTACCAG GTCTCCTGTT AAGAAGTCCA CACCAACCCC AACCCAGACA 
CAGAGGGAGA CAGCGAATGT TTTGGCAGAA TTAGCAGATG AGGGATGCTC CACACAAGAG 
GTGCAGGAGG TGTTTACAGA AGGACAGACA CTGTTGCCTG AACCTAGTGG CTTCGTACCA 
GACAGGACCC CACTTTCTCC TCAGAAGATG CCTAGCTCTG TCCCAAGTAC AACCCTGACC 
AAGCTGTCTG CAGACCTCCT GGGAATGGTG AACAGTCCCA AACTCAGTGA CTTAAACCTG 
CTGTGCAGAA ATGGGGAAGT GATACACGTG CACAAGTTTG TCCTTGTCGC ACGGTGTCCT 
GTGATAGCAA AGATGCTGGA GGTGAAAGAT GACAAAGACT TAGCTGCACT GGACTTCTCT 
GACTATGAAA AGGATACAGT ACTAACTACT CTAGAGTACA TGTATGGTGG AAAGATCTCC 
CTGAGTGATG CAGTTTTGGC TGCAAATGTG CTGAGGGTGG CTCAAAAGCT GGAGCTGAAG 
GAACTGGAGG ACAGCTGCAA GGCCTTCCTG AAACCCACGT TTTCAACTGT TTCCTCATCT 
CCAGAATTCC AAGAAAAAGA TCTGCCTGAA CTTCTGGACA CCATGTTAGG CTCACCAACC 
AGCAGCCCAA ACCTTTCACA AGGCAGTGTG AATCGAAGCT CTGACAGTCA GGGATTTAAT 
GACTCGGAGT TGGAAGACAT CGCCTTGAGT CAGAAGCAAA GGTTTCAGAA GGCAAGAACA 
TTGCCGTCAA AAAACGTTAA CAAGGAGCCT GCAGCTACAA AGAAACCTAC CCAGATGGTT 
CCCCCTGCAG GAAGAGGCCT TGGACAGGCT CCTGAGAGAC AAGAAACTGG CCTCCATGTT 
CCAGTCATGA CCATCGGAGA TGTGGTAGAA TCTGCAATTG TGAAGAACGT AAAACAGCCC 
ACCAGAATCG GTCCTGATGC CAGCCCAACG TCGGCCCCAG CTCACCAACC AATACTTACA 
AGCACAGGGC AGACAGCTGG TGTTGGCGTT AGGATTCCCG TTGAGCACAG CTATTCGACC 
AGCCCTAGGA AGGGAACACA GAGAGGGCGC CGAGGGCCAA AGACAACTGT TGCACCAATG 
TTAGAAGGGT CCGCAACAAC CGTAAGTCCA GCGAAACAAC CAGCATCCTT AGCACCTTCA 
ATGCAAGGCA TAGCAACAAC TGTGATGACT TCAACACATG GCCTAACAAC AACTGTGATG 
CCTTCAACAC ATGCTGTGAC ATCAACAGCA ACTCTGTCAT CATCACAACT TGGTGTGGCA 
ACAGCTGTTA CACAAGCTAG CCCTGCTCAA ACTACCATGG GCAGCCACAG CGCATTGCCC 
CGACCAGGAG CCAGGGAACA GTCCCTCATC ATCCAGTCAC AAGAGCTTTC CAACAAGATA 
GGTTGCCCGG AGTGCGAGAA ATCAGGGGTA CGAGTGTCTG CAAATGAGGC ACAGATGCTC 
ATCCAGGGTG GCCCAGCTGC AAACGCCATG CGACAGTTGA TCATTGCGAG ACAGAGGCCC 
TGCCCGCACG GGAAGTACCC TTCTTGGGGC TCTCAAGTTG CTCCAACATG GATGCCCCCA 
CATGCACCAG AAGCTCTCCA GGGCCGATCT GCAGGACAAC TGTTGGCTGG TCTTCGATCC 
CGTACACCCC CTAACAGTCC CCTGCGGCAG ACTGCAGTAG CTACAGTAGT CACACCGGCT 
GCTCCTGGAC ACACACACAC AAGTCCAGCC TATACTCAAA GTCCATCTGG TGCAAGAATG 
CAGAACTTTG CAGAGTTTAA TAGCCCACCT TTTGGACCAG GGAGACCACA ACCTGATGTA 
AGACTACCTG GGAGTAGACC ACCAATACCC CCACTGCCTG GATTTGGAAT ACTTCCACCT 
GGTGGCAGAC TTCCACCAGC ATCAACTTTA CTCCCTAGAA CGAACATTCT TGCTCAGCTG 
AGAGAACCCT CTCAATTTGC CCAGCAAAGA TATGGCCCAC AGGGTGTTGT TCCGGAGCAG 
TTTCCTACAC AGAGGAGTCC GAGAGGTCCT GCCATATCTC CCATAAGCTC ACAGCGCAGT 
GGAAAAGAAC TGGATTCCCC GACATCATCC CAGAATGGCT CTGAGTTGAT TCCCTCCAGG 
AGACTGCTCT TGCATACAGC GCAGAGGATG TTACCTGGTA ACCAGTACCT CAGCAATCAG 
TTTCTCTCTC CAACTGGCCT CCAGCGGCCA ACACTTCACG ACAACACACC TCGGCTTTCT 
GTTGTGGCTG AGTCTAGCCC ACCACCCAAC ACTTCAACTG GTGTGGCTAT CATCCGAATC 
CCCTCTCCAA TGGAGAAAAG CGGCCAAGGC AGAAGTGAGC GACATAGTTG GTCAAGTTTG 
GTAAGGAGAG AGAGAAGTAC AGATGACTGT AGCATTCTCA AGGTCTTGTT GCAAAAAGGG 
GTGGGTGCAG CCATTCCGCA GCTGTATAAG GATCAGGAAG TCCCCCCTCA TATTCAGAAG 
CAGGTTTTGA AAGAAACACG ATTGAAAAGA CTAATGCCAC AAACTAAGCA GCATGATGTG 
CAGCCAGCCT CTCAAGCATG TGGTGAAATG GTGGTGGGTA GGAACTGTAG TGGTGAAGTG 
CATGGTGGAA TAGTAGTGGC TGATACTAAC ATTCGTAAGC AAATGGTGAC TAGAGAGAGT 
GGTGCGGGAC TGCATGACAG TCATTCAGGT GCACAGAAAC AGTGTAGAGA GGTGGTGAAA 
GGTGTGGAAC ATGGTGGAAG CCTTGTGAAA CAAGGAAAGG ATGAACTTGC AGGGGAAGAG 
AGTGGTACTG AATCTCTGTC ATGTCCAACT GCTGATGAGT TAAGGGACAG CATGCTATTT 
AGAATTGTAC ACCCTGAAAC TTTCAGTCCA GTGCCAAAAA TAAAGGGTAA ACCAGTGCAT 
GATGGAGGTG AAGTGGGCAA GGTGCAGGGT AAAGAGTTGC ATGCTGTGAA ACTAACACGT 
GAGAAAGTAC TAACAAAGGA CTGCAGTCCA ATGGTTGGTG AGGGAAGTCG TCATCAGAAG 
GAAGGTAACG GTGTGGCTGC TGTGTGTGAC AGTGACTCAA GTGTACAGAA TGTTGTAAAA 
CAATGTACTA CTTCAAAAGT AGAGGAGGAC ACAGACTGTG ATGAACTTCG TTCCACTTTT 
ACAGGTCCAA ATGCAAAGCA AAGAAGAGGA AAAGCAACAT CAGCTACAAC ATTCCCTTCA 
AGCAGCCCAG ACACAATGAG TAGACATTAT ACATCTCCAA TGTTGAGGGG ATCAGTGGAA 
ATAGTGGTGA ATTCTGACAG AGAACAGCCA GCTTCGGAAA GACAAGAACC TGATATAGGC 
AAAGTTCCTA GTATTTTTGA CAACAACCAG GGTGTGTCCA TCCCCAGGGA CCAGGGAAAC 
AACAGAGTAA GTCGGAGAGC GTCACAAAGA AAGAACCAGA CCACGCCTAC TGAGGCAACA 
ATGCCATCAT CACAGGCGAC AAATTCTGTA CAGACCAGAA GCAAAGGTGG CAAACAAAAA 
GGTGAAACAA CACCTTCACC TACCAAACGT CTACGGATGA GTCGACCACC AGAAGGGAAG 
AGGCATGCTT GTACTCAGTC ACCAAAGAAG TTTTGGACTG ACTACCTTAA AGCTCAGTTG 
GCTAGTGGTA ATGCTCCAGC TGGGCCAGTA GGTGAGTCAA TGATATCGAT GCAGACGCCA 
GATTTAAGTG CTGTAAAACC TGTTAGAAGA GGCATCTCCT CACCAGAACT GGCTCTGACA 
ACTCCAGGAG TGACTGGTTC AACAACCTTC CAGCCAAAGT TTTCCACCCC TGTGGGCAGC 
CACACCCAAC CAGTAGGTTC CCCTCGACTT AGCCTTGACC AGGAGCTAAA GGAAGCAGAG 
ACAGAAACAG TGTTCCATGG AACAGGTCCA GAACCTTATG CTCCAACAGT TGAAAAGAAG 
ACCTCATTGG ACTCCGGGTC TCAGCAAGCT GATGATGCCA CTGGCAAAGA TGTAGATGAT 
GATGTTGAAT GCATTGCAGG CAACTCAGTT GATGATGTTG GCTTGGGAGA TGTAGAGAAG 
ATTGACCTTA CACAGGAACA TGCTGATGAA ACTGGTGCAG AGCCTCAGCA GGGTGTTTGT 
GGAACAGAAC ATGGGTGTGG GACTCCTGAG CATGAACAGC CTTCCTCTCC CAGCTCTCAG 
AGCAAGCCAG CAGAGGAACA ACAGTCCCAG AGCTCTCAGC CCTCTGTGGT GAACGACAGT 
TTAATGGACT TCTTCGATGA CGGAGGTTAC ATGGAAGTGC CTTTTGGTGG AGAAGTTGAG 
TGCGCTGCTG ACTCTTCTGA TGACAAAATC AACCTGAGCA GTGTAACCAG CAAAACTGAA 
TCAAAAGATG ACAAGAATGC TGATGAGTTG AGTCCTAGTT TAGATGCAAC AAAGGACAAT 
GATACCACAG CTTCAGTGCC TTTATCACCG AAAAATGCCT CCACTGTCAT TGAGGAATTC 
CCAGACACCC AGCAAGAACC TGTCCATGTT GAAGAAGATG CACACACAGA TCAATATGTT 
GTAGAAGTTG TTGAGGAGTC CCCAACAGCA GGAGAGGATG TAGTGAACAA TAGCATGTAT 
GATTTGGAAC CCTATCATGA AGACCATTTT TGGGATGACA GCAATGAACC ACAACCTGAT 
GAATCAACAC CTGTACATAG TGTGGTGGGG AGCGGAGAAA CTGGAACAGG TGCTGTGAAG 
TCCACTCCAC TGCAGACACA TCGCAAGAGC AAGGCCCCTG TGGTTCCCAT TACTCCCATG 
CCTGATTACC CCAACATGGA AACACCAGAA CTTAAGAAAC AAGTCAAGAA ATTTGGAGTG 
CGGGTTCTGA AGAAGCCACA AATGGTGGCA AAGCTGACAG AAATCTACCA GTATACACAT 
CAAGAGCTCC CATTGGTTGA TGGTGGAAGG TCCCCATTTG TTTTCTGTTT GGTCTCGGCT 
GTCTGCCGTT TCTTCCTCTC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002585778.1
CDS	1..7287
Translation	MSGNIKSVMNVKASKVNLNPSVLLRKLSDEEVLRNTTPEKFHKTGKQRASVSADTEKSFEVRQSKSDSERKQDSQKKVEVTKPLSKSTEQNPSQAKPSTATEVPQKKVAKPASEAKAQHKPTEGSVIESVKDVVGKETSAPSRSTVTEDLLTQISLSAPVKEVKKVVQVLTLDDIPGKSDKSEGKRSQLIEKDSQKVSAPKEMTTMMTESRNLSVVVPKLPMHISPQKSYNINDPSITPSKAKPRTPTAKEALARKMVKKAKTRANGKGAALNFKRLIGENGNLDNSSDFVETGKTFQPAVRKLRSRAVKKAAKDKVRKPASPDTTVSTEKGPGKTSSVPVNVENKEGKTCLTCKQTIFGTEEAVKEHMKTCLRQRFVSDKHSSRHTGLRHLPEPAEEGLMDLEVSDDESFARHLQQREEDKLMEEQLTDAEFYFCQICGRDLTHLNTVRRTQHINRCIDAMETEKKLHAEQNIKDVPDCPICGKVLESRAQRQSHLKRCAVQYGVTTAQLLELVKKQEEEKEARRKNQPRSQLQPAQQQQREMDERTQVAMAMSMSLVEKEGSREQPTVTSALPVGGKESKRRKKAAKTDVVPALLVTSKEDREKTLARKVASIIVQGDQESHPKTPSLRKSKLGRKYHTARKTMGKGPSSPSFLQKVGAAMTSSPPAASTSTAASPASSQEKAPLWSMTKRIDEKKTDDFYVQELSKQITPAKYVPVTPMRKAAKKAMLTFTKKNDTSEDETTDGPPQKAVAGSQKAKKTPTATRSPVKKSTPTPTQTQRETANVLAELADEGCSTQEVQEVFTEGQTLLPEPSGFVPDRTPLSPQKMPSSVPSTTLTKLSADLLGMVNSPKLSDLNLLCRNGEVIHVHKFVLVARCPVIAKMLEVKDDKDLAALDFSDYEKDTVLTTLEYMYGGKISLSDAVLAANVLRVAQKLELKELEDSCKAFLKPTFSTVSSSPEFQEKDLPELLDTMLGSPTSSPNLSQGSVNRSSDSQGFNDSELEDIALSQKQRFQKARTLPSKNVNKEPAATKKPTQMVPPAGRGLGQAPERQETGLHVPVMTIGDVVESAIVKNVKQPTRIGPDASPTSAPAHQPILTSTGQTAGVGVRIPVEHSYSTSPRKGTQRGRRGPKTTVAPMLEGSATTVSPAKQPASLAPSMQGIATTVMTSTHGLTTTVMPSTHAVTSTATLSSSQLGVATAVTQASPAQTTMGSHSALPRPGAREQSLIIQSQELSNKIGCPECEKSGVRVSANEAQMLIQGGPAANAMRQLIIARQRPCPHGKYPSWGSQVAPTWMPPHAPEALQGRSAGQLLAGLRSRTPPNSPLRQTAVATVVTPAAPGHTHTSPAYTQSPSGARMQNFAEFNSPPFGPGRPQPDVRLPGSRPPIPPLPGFGILPPGGRLPPASTLLPRTNILAQLREPSQFAQQRYGPQGVVPEQFPTQRSPRGPAISPISSQRSGKELDSPTSSQNGSELIPSRRLLLHTAQRMLPGNQYLSNQFLSPTGLQRPTLHDNTPRLSVVAESSPPPNTSTGVAIIRIPSPMEKSGQGRSERHSWSSLVRRERSTDDCSILKVLLQKGVGAAIPQLYKDQEVPPHIQKQVLKETRLKRLMPQTKQHDVQPASQACGEMVVGRNCSGEVHGGIVVADTNIRKQMVTRESGAGLHDSHSGAQKQCREVVKGVEHGGSLVKQGKDELAGEESGTESLSCPTADELRDSMLFRIVHPETFSPVPKIKGKPVHDGGEVGKVQGKELHAVKLTREKVLTKDCSPMVGEGSRHQKEGNGVAAVCDSDSSVQNVVKQCTTSKVEEDTDCDELRSTFTGPNAKQRRGKATSATTFPSSSPDTMSRHYTSPMLRGSVEIVVNSDREQPASERQEPDIGKVPSIFDNNQGVSIPRDQGNNRVSRRASQRKNQTTPTEATMPSSQATNSVQTRSKGGKQKGETTPSPTKRLRMSRPPEGKRHACTQSPKKFWTDYLKAQLASGNAPAGPVGESMISMQTPDLSAVKPVRRGISSPELALTTPGVTGSTTFQPKFSTPVGSHTQPVGSPRLSLDQELKEAETETVFHGTGPEPYAPTVEKKTSLDSGSQQADDATGKDVDDDVECIAGNSVDDVGLGDVEKIDLTQEHADETGAEPQQGVCGTEHGCGTPEHEQPSSPSSQSKPAEEQQSQSSQPSVVNDSLMDFFDDGGYMEVPFGGEVECAADSSDDKINLSSVTSKTESKDDKNADELSPSLDATKDNDTTASVPLSPKNASTVIEEFPDTQQEPVHVEEDAHTDQYVVEVVEESPTAGEDVVNNSMYDLEPYHEDHFWDDSNEPQPDESTPVHSVVGSGETGTGAVKSTPLQTHRKSKAPVVPITPMPDYPNMETPELKKQVKKFGVRVLKKPQMVAKLTEIYQYTHQELPLVDGGRSPFVFCLVSAVCRFFLSL

Target ORF information:

RefSeq Version	XM_002585732.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002585732.1

ORF Insert Sequence:

ATGTCAGGCA ATATCAAAAG TGTGATGAAC GTTAAGGCAA GTAAAGTAAA CCTAAACCCC 
AGCGTTTTGC TAAGAAAGCT CTCAGATGAG GAAGTGTTGA GAAATACAAC GCCAGAGAAA 
TTTCACAAAA CAGGAAAGCA GAGGGCAAGT GTTAGTGCCG ATACGGAAAA AAGCTTCGAA 
GTGCGACAGT CAAAGAGTGA TAGTGAGAGA AAGCAGGACT CTCAAAAGAA GGTTGAAGTA 
ACCAAGCCAC TGTCAAAAAG CACTGAGCAA AATCCTAGTC AAGCAAAACC CAGTACAGCA 
ACAGAAGTAC CACAAAAGAA AGTAGCAAAG CCAGCCAGTG AGGCTAAAGC ACAGCACAAA 
CCAACAGAAG GTAGTGTAAT AGAATCAGTG AAAGACGTGG TTGGTAAAGA GACTTCCGCA 
CCCTCCCGTA GTACAGTAAC AGAAGACCTG CTGACTCAGA TCAGTCTATC AGCCCCAGTA 
AAGGAAGTAA AGAAAGTCGT CCAAGTCCTG ACTTTGGATG ACATCCCAGG CAAAAGTGAC 
AAATCAGAAG GAAAAAGGTC TCAACTGATT GAAAAGGATA GTCAGAAAGT GTCAGCTCCA 
AAAGAAATGA CGACCATGAT GACAGAAAGT CGCAATCTGA GTGTTGTGGT ACCAAAGCTA 
CCGATGCACA TCAGTCCTCA GAAGTCATAC AACATCAATG ACCCAAGCAT CACCCCAAGC 
AAAGCCAAGC CCAGGACCCC AACAGCAAAG GAAGCACTCG CTCGGAAGAT GGTGAAGAAG 
GCAAAGACTC GAGCAAACGG GAAGGGAGCA GCGCTAAATT TCAAGCGTCT CATCGGAGAG 
AATGGAAATT TGGACAACAG CAGTGACTTT GTGGAAACAG GGAAAACTTT TCAGCCCGCT 
GTACGCAAGT TACGCAGTCG CGCAGTGAAG AAGGCTGCCA AAGATAAAGT ACGCAAACCC 
GCGAGTCCTG ACACAACAGT TTCTACAGAG AAGGGCCCTG GCAAGACTAG CAGTGTGCCA 
GTTAATGTAG AAAACAAGGA GGGGAAAACC TGTCTAACAT GCAAACAGAC CATATTTGGT 
ACAGAAGAGG CAGTGAAGGA ACACATGAAG ACTTGTTTGA GACAGCGGTT TGTGTCAGAC 
AAACATTCCT CAAGACATAC TGGTCTAAGA CACCTGCCTG AACCTGCAGA AGAGGGCCTA 
ATGGACTTAG AGGTGTCTGA TGATGAGAGT TTTGCCCGCC ACTTACAGCA GAGGGAGGAA 
GACAAATTGA TGGAAGAGCA GCTTACAGAT GCAGAGTTCT ACTTCTGCCA GATCTGTGGA 
CGTGATCTGA CTCATCTCAA CACTGTCCGG AGAACGCAGC ACATCAACCG CTGTATTGAC 
GCCATGGAGA CGGAGAAAAA ACTGCACGCA GAGCAGAACA TTAAAGATGT TCCTGACTGT 
CCCATCTGTG GCAAAGTGCT GGAAAGCAGG GCACAGAGAC AGTCCCACCT GAAGCGCTGT 
GCTGTACAGT ACGGGGTGAC CACGGCACAG CTGCTGGAAC TGGTGAAAAA GCAAGAGGAA 
GAGAAAGAGG CTCGCAGAAA GAACCAACCC AGGTCTCAAC TACAGCCAGC TCAACAGCAG 
CAAAGGGAAA TGGATGAACG GACCCAGGTT GCCATGGCAA TGTCTATGTC ACTTGTGGAG 
AAGGAAGGAT CCAGAGAGCA GCCAACAGTC ACGTCTGCAC TCCCTGTAGG TGGGAAGGAA 
AGCAAGAGAA GGAAGAAGGC TGCAAAGACT GATGTAGTAC CAGCTCTGTT GGTGACAAGT 
AAAGAGGATA GAGAAAAAAC TCTGGCTCGA AAAGTGGCCT CTATCATTGT GCAGGGTGAC 
CAGGAATCTC ACCCAAAGAC TCCAAGCCTG AGAAAAAGCA AACTGGGTAG GAAGTACCAC 
ACAGCTAGGA AGACCATGGG TAAGGGCCCA TCCTCCCCCA GCTTCCTACA GAAAGTTGGT 
GCAGCTATGA CCAGCAGCCC CCCTGCAGCT TCCACCAGTA CTGCAGCATC TCCTGCCTCC 
AGCCAGGAGA AGGCACCCCT GTGGTCCATG ACGAAGCGTA TTGACGAGAA GAAGACTGAT 
GACTTCTATG TTCAGGAGTT GTCCAAGCAG ATCACTCCAG CTAAGTATGT ACCCGTCACA 
CCAATGAGGA AGGCAGCCAA GAAAGCAATG CTGACGTTTA CGAAGAAAAA TGACACAAGC 
GAGGATGAAA CAACTGACGG CCCCCCACAG AAGGCTGTTG CGGGCAGCCA AAAGGCTAAG 
AAAACTCCCA CTGCTACCAG GTCTCCTGTT AAGAAGTCCA CACCAACCCC AACCCAGACA 
CAGAGGGAGA CAGCGAATGT TTTGGCAGAA TTAGCAGATG AGGGATGCTC CACACAAGAG 
GTGCAGGAGG TGTTTACAGA AGGACAGACA CTGTTGCCTG AACCTAGTGG CTTCGTACCA 
GACAGGACCC CACTTTCTCC TCAGAAGATG CCTAGCTCTG TCCCAAGTAC AACCCTGACC 
AAGCTGTCTG CAGACCTCCT GGGAATGGTG AACAGTCCCA AACTCAGTGA CTTAAACCTG 
CTGTGCAGAA ATGGGGAAGT GATACACGTG CACAAGTTTG TCCTTGTCGC ACGGTGTCCT 
GTGATAGCAA AGATGCTGGA GGTGAAAGAT GACAAAGACT TAGCTGCACT GGACTTCTCT 
GACTATGAAA AGGATACAGT ACTAACTACT CTAGAGTACA TGTATGGTGG AAAGATCTCC 
CTGAGTGATG CAGTTTTGGC TGCAAATGTG CTGAGGGTGG CTCAAAAGCT GGAGCTGAAG 
GAACTGGAGG ACAGCTGCAA GGCCTTCCTG AAACCCACGT TTTCAACTGT TTCCTCATCT 
CCAGAATTCC AAGAAAAAGA TCTGCCTGAA CTTCTGGACA CCATGTTAGG CTCACCAACC 
AGCAGCCCAA ACCTTTCACA AGGCAGTGTG AATCGAAGCT CTGACAGTCA GGGATTTAAT 
GACTCGGAGT TGGAAGACAT CGCCTTGAGT CAGAAGCAAA GGTTTCAGAA GGCAAGAACA 
TTGCCGTCAA AAAACGTTAA CAAGGAGCCT GCAGCTACAA AGAAACCTAC CCAGATGGTT 
CCCCCTGCAG GAAGAGGCCT TGGACAGGCT CCTGAGAGAC AAGAAACTGG CCTCCATGTT 
CCAGTCATGA CCATCGGAGA TGTGGTAGAA TCTGCAATTG TGAAGAACGT AAAACAGCCC 
ACCAGAATCG GTCCTGATGC CAGCCCAACG TCGGCCCCAG CTCACCAACC AATACTTACA 
AGCACAGGGC AGACAGCTGG TGTTGGCGTT AGGATTCCCG TTGAGCACAG CTATTCGACC 
AGCCCTAGGA AGGGAACACA GAGAGGGCGC CGAGGGCCAA AGACAACTGT TGCACCAATG 
TTAGAAGGGT CCGCAACAAC CGTAAGTCCA GCGAAACAAC CAGCATCCTT AGCACCTTCA 
ATGCAAGGCA TAGCAACAAC TGTGATGACT TCAACACATG GCCTAACAAC AACTGTGATG 
CCTTCAACAC ATGCTGTGAC ATCAACAGCA ACTCTGTCAT CATCACAACT TGGTGTGGCA 
ACAGCTGTTA CACAAGCTAG CCCTGCTCAA ACTACCATGG GCAGCCACAG CGCATTGCCC 
CGACCAGGAG CCAGGGAACA GTCCCTCATC ATCCAGTCAC AAGAGCTTTC CAACAAGATA 
GGTTGCCCGG AGTGCGAGAA ATCAGGGGTA CGAGTGTCTG CAAATGAGGC ACAGATGCTC 
ATCCAGGGTG GCCCAGCTGC AAACGCCATG CGACAGTTGA TCATTGCGAG ACAGAGGCCC 
TGCCCGCACG GGAAGTACCC TTCTTGGGGC TCTCAAGTTG CTCCAACATG GATGCCCCCA 
CATGCACCAG AAGCTCTCCA GGGCCGATCT GCAGGACAAC TGTTGGCTGG TCTTCGATCC 
CGTACACCCC CTAACAGTCC CCTGCGGCAG ACTGCAGTAG CTACAGTAGT CACACCGGCT 
GCTCCTGGAC ACACACACAC AAGTCCAGCC TATACTCAAA GTCCATCTGG TGCAAGAATG 
CAGAACTTTG CAGAGTTTAA TAGCCCACCT TTTGGACCAG GGAGACCACA ACCTGATGTA 
AGACTACCTG GGAGTAGACC ACCAATACCC CCACTGCCTG GATTTGGAAT ACTTCCACCT 
GGTGGCAGAC TTCCACCAGC ATCAACTTTA CTCCCTAGAA CGAACATTCT TGCTCAGCTG 
AGAGAACCCT CTCAATTTGC CCAGCAAAGA TATGGCCCAC AGGGTGTTGT TCCGGAGCAG 
TTTCCTACAC AGAGGAGTCC GAGAGGTCCT GCCATATCTC CCATAAGCTC ACAGCGCAGT 
GGAAAAGAAC TGGATTCCCC GACATCATCC CAGAATGGCT CTGAGTTGAT TCCCTCCAGG 
AGACTGCTCT TGCATACAGC GCAGAGGATG TTACCTGGTA ACCAGTACCT CAGCAATCAG 
TTTCTCTCTC CAACTGGCCT CCAGCGGCCA ACACTTCACG ACAACACACC TCGGCTTTCT 
GTTGTGGCTG AGTCTAGCCC ACCACCCAAC ACTTCAACTG GTGTGGCTAT CATCCGAATC 
CCCTCTCCAA TGGAGAAAAG CGGCCAAGGC AGAAGTGAGC GACATAGTTG GTCAAGTTTG 
GTAAGGAGAG AGAGAAGTAC AGATGACTGT AGCATTCTCA AGGTCTTGTT GCAAAAAGGG 
GTGGGTGCAG CCATTCCGCA GCTGTATAAG GATCAGGAAG TCCCCCCTCA TATTCAGAAG 
CAGGTTTTGA AAGAAACACG ATTGAAAAGA CTAATGCCAC AAACTAAGCA GCATGATGTG 
CAGCCAGCCT CTCAAGCATG TGGTGAAATG GTGGTGGGTA GGAACTGTAG TGGTGAAGTG 
CATGGTGGAA TAGTAGTGGC TGATACTAAC ATTCGTAAGC AAATGGTGAC TAGAGAGAGT 
GGTGCGGGAC TGCATGACAG TCATTCAGGT GCACAGAAAC AGTGTAGAGA GGTGGTGAAA 
GGTGTGGAAC ATGGTGGAAG CCTTGTGAAA CAAGGAAAGG ATGAACTTGC AGGGGAAGAG 
AGTGGTACTG AATCTCTGTC ATGTCCAACT GCTGATGAGT TAAGGGACAG CATGCTATTT 
AGAATTGTAC ACCCTGAAAC TTTCAGTCCA GTGCCAAAAA TAAAGGGTAA ACCAGTGCAT 
GATGGAGGTG AAGTGGGCAA GGTGCAGGGT AAAGAGTTGC ATGCTGTGAA ACTAACACGT 
GAGAAAGTAC TAACAAAGGA CTGCAGTCCA ATGGTTGGTG AGGGAAGTCG TCATCAGAAG 
GAAGGTAACG GTGTGGCTGC TGTGTGTGAC AGTGACTCAA GTGTACAGAA TGTTGTAAAA 
CAATGTACTA CTTCAAAAGT AGAGGAGGAC ACAGACTGTG ATGAACTTCG TTCCACTTTT 
ACAGGTCCAA ATGCAAAGCA AAGAAGAGGA AAAGCAACAT CAGCTACAAC ATTCCCTTCA 
AGCAGCCCAG ACACAATGAG TAGACATTAT ACATCTCCAA TGTTGAGGGG ATCAGTGGAA 
ATAGTGGTGA ATTCTGACAG AGAACAGCCA GCTTCGGAAA GACAAGAACC TGATATAGGC 
AAAGTTCCTA GTATTTTTGA CAACAACCAG GGTGTGTCCA TCCCCAGGGA CCAGGGAAAC 
AACAGAGTAA GTCGGAGAGC GTCACAAAGA AAGAACCAGA CCACGCCTAC TGAGGCAACA 
ATGCCATCAT CACAGGCGAC AAATTCTGTA CAGACCAGAA GCAAAGGTGG CAAACAAAAA 
GGTGAAACAA CACCTTCACC TACCAAACGT CTACGGATGA GTCGACCACC AGAAGGGAAG 
AGGCATGCTT GTACTCAGTC ACCAAAGAAG TTTTGGACTG ACTACCTTAA AGCTCAGTTG 
GCTAGTGGTA ATGCTCCAGC TGGGCCAGTA GGTGAGTCAA TGATATCGAT GCAGACGCCA 
GATTTAAGTG CTGTAAAACC TGTTAGAAGA GGCATCTCCT CACCAGAACT GGCTCTGACA 
ACTCCAGGAG TGACTGGTTC AACAACCTTC CAGCCAAAGT TTTCCACCCC TGTGGGCAGC 
CACACCCAAC CAGTAGGTTC CCCTCGACTT AGCCTTGACC AGGAGCTAAA GGAAGCAGAG 
ACAGAAACAG TGTTCCATGG AACAGGTCCA GAACCTTATG CTCCAACAGT TGAAAAGAAG 
ACCTCATTGG ACTCCGGGTC TCAGCAAGCT GATGATGCCA CTGGCAAAGA TGTAGATGAT 
GATGTTGAAT GCATTGCAGG CAACTCAGTT GATGATGTTG GCTTGGGAGA TGTAGAGAAG 
ATTGACCTTA CACAGGAACA TGCTGATGAA ACTGGTGCAG AGCCTCAGCA GGGTGTTTGT 
GGAACAGAAC ATGGGTGTGG GACTCCTGAG CATGAACAGC CTTCCTCTCC CAGCTCTCAG 
AGCAAGCCAG CAGAGGAACA ACAGTCCCAG AGCTCTCAGC CCTCTGTGGT GAACGACAGT 
TTAATGGACT TCTTCGATGA CGGAGGTTAC ATGGAAGTGC CTTTTGGTGG AGAAGTTGAG 
TGCGCTGCTG ACTCTTCTGA TGACAAAATC AACCTGAGCA GTGTAACCAG CAAAACTGAA 
TCAAAAGATG ACAAGAATGC TGATGAGTTG AGTCCTAGTT TAGATGCAAC AAAGGACAAT 
GATACCACAG CTTCAGTGCC TTTATCACCG AAAAATGCCT CCACTGTCAT TGAGGAATTC 
CCAGACACCC AGCAAGAACC TGTCCATGTT GAAGAAGATG CACACACAGA TCAATATGTT 
GTAGAAGTTG TTGAGGAGTC CCCAACAGCA GGAGAGGATG TAGTGAACAA TAGCATGTAT 
GATTTGGAAC CCTATCATGA AGACCATTTT TGGGATGACA GCAATGAACC ACAACCTGAT 
GAATCAACAC CTGTACATAG TGTGGTGGGG AGCGGAGAAA CTGGAACAGG TGCTGTGAAG 
TCCACTCCAC TGCAGACACA TCGCAAGAGC AAGGCCCCTG TGGTTCCCAT TACTCCCATG 
CCTGATTACC CCAACATGGA AACACCAGAA CTTAAGAAAC AAGTCAAGAA ATTTGGAGTG 
CGGGTTCTGA AGAAGCCACA AATGGTGGCA AAGCTGACAG AAATCTACCA GTATACACAT 
CAAGAGCTCC CATTGGTTGA TGGTGGAAGG TCCCCATTTG TTTTCTGTTT GGTCTCGGCT 
GTCTGCCGTT TCTTCCTCTC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_111136 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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