BRAFLDRAFT_82678 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_82678
Entrez Gene ID	7239510
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002601334.1	XP_002601380.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_82678 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_82678 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb14492	XM_002601334.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_82678 ( XM_002601334.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002601380.1 Branchiostoma floridae hypothetical protein, mRNA.

Price & Availability↑

CloneID	OBb14492
Clone ID Related Accession (Same CDS sequence)	XM_002601334.1
Accession Version	XM_002601334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101478). COMPLETENESS: incomplete on both ends.

ATGACTGCGA AGACAGTTCT CTTCTTTTGG CTCTTCGTGG TGCTCTCAAA ACAAGCCACT 
GCTTGTGACT CCGCCATAGA CGCTGGATTT ACACCTACAG GAGTGATTAC GTCACCAAAC 
TACCCATCCA CGTATCCTAA AGATACACAA TGTACCTGGA CTATTACTGC ACTTGCTGGT 
CAGGCTGTTA CCTTGGACAT AACTGACTTC AACCTAGCTG GTTATAACTG TGGCACTGGG 
GACACACTTG AGGTTTTTGA CGGCAGTTCT GATGCAACGA CTCTACTGGA CACGTATTGT 
GTGGGAAATA CGCCTGATGG ACAGATAGTG TCTACAGGAC CATCAATGAC TGTTGTCTTT 
ACATCGCTCG GAAACAACTT TTTCAGCAAC GGATTCAGTG CCACGTATGG AACTACTATA 
GGACAAGCCT CTACTGAGAA AGTTGGATAC GAGGCTTGGA GGGGTCGTCT GAATAACGGC 
AAGGCGTGGC AGACCGACAC AGCCGATACA AACCAGTGGC TACAGATCGA CCTAGGTAGT 
CAAAAGTTCG TCACCGGCAT CCAAACACAA GGTCTGTGGG GAGGGCACAC CAAGACTTAC 
AAACTGTTGT ACTCTGACGA TGGGACTACG TGGGTCACTT ACGGAGATGG ACAAGATGAC 
ACCATCTTTA CAGGTAACAC CGACGGCCCA ACAGTAGTGC AACAAACCCT ACCAACTCCA 
GTTCTCACAC AGTACGTGAG GATAAATCCA CAAACATTTC AGGGCACCGC TTACATCCGT 
CTGCGGGCAG AGATACTTGG CTGTGATGAT ATAGACGCCT GCAGTCCTGA CCCCTGTACA 
CACGGCACCT GTACAGACGG TGTAGCGAGC TACACTTGTA GTTGTGAGAG CGGCTGGGAT 
GGTCAAAACT GTGACCAAGA TATAGACGAC TGTGCAACCT CCCCCTGTCT ACACGGCACC 
TGTACAGACG GAGCAGCGAG CTACACTTGT AGTTGTGAGA ACGGCTGGGA AGGTGTCAAT 
TGTGATCAAG ATATAGACGA CTGTGCGGCC TCTCCCTGTA CACACGGCAC CTGTACAGAC 
GGTGTAGCGA GCTACACTTG TAGTTGTGAG AACGGCTGGG AAGGTCAAAA CTGTGATCAA 
AATACATACG ACTGCAGTCC CAACCCCTGT CTTCACGGCA CCTGTACAGA CGGTGTAGCG 
AGCTACAATT GTAGTTGTGA GAACGGCTGG GAGGGTGAAA ACTGTGACCA AGACATAGAC 
GACTGCAGTC CCAACCCCTG TCTCCACGGC ACCTGTACAG ACGGTGTAGC GAGCTACACT 
TGTAGTTGTG AGAGCGGCTG GGAAGGTCAA AGCTGTGATC AAGATATAGA CGATTGTGCG 
GCCTCTCCCT GTCTCCACGG CACCTGTACA GACGGTGTAG CGAGCTACAC TTGTAGTTGT 
GAGAACGGCT GGGAAGGTGT CAATTGTGAT CAAGATATAG ACGACTGTGC GGCCTCTCCC 
TGTACACACG GTACCTGTAC AGACGGTGTA GCGAGCTACA CTTGTAGTTG TGAGAACGGC 
TGGGAAGGTC AAAGCTGTGA TCAAAATACA GACGACTGCA GTCCCAACCC CTGTCTCCAC 
GGCACCTGTA CAGACGGTGT AGCGAGCTAC ACTTGTAGTT GTGAGAGCGG CTGGGAAGGT 
CAAAGCTGTG ATCAAGACAT AGACGACTGT GGGGCCTCTC CCTGTCTCCA TGGCACCTGT 
ACAGACGGTG TAGCGAGCTA CACTTGTAGT TGTGAGAACG GCTGGGAAGG TCAAAATTGT 
GATCAAGATA TAGATGACTG CAGTCCCAAC CCCTGTCTCC ACGGCACCTG TACAGACGGT 
GTAGCTAGCT ACACTTGTAG TTGTGAGAAC GGCTGGGAAG ACATAGACGA CTGTGCGGCC 
TCCCCCTGTC GCCACGGCAC CTGTACGGAC GGTGTAGCGA GTTACACTTG TAGTTGTGAG 
AACGGCTGGG AAGGGGGCAA CTGTGATCAG GACATAGACG ACTGTGCGAC CTCTCCCTGT 
ACACACGGTA CCTGTACAGA CGGTGTAGCG AGCTACACTT GTAGTTGTGA GAACGGATGG 
GGAGGGGACA ACTGTGATCA AAATATAGAC GACTGCAGTC CTGACCCCTG TACACACGGC 
ACCTGTCATG ACGCAGTAGC CGGTTATACC TGTACCTGTG AGCACGGTTG GGGCGGAGTC 
AACTGTAGTA ATAATATTGA CAATTGTGTT GGCAACCCCT GCCTCCACGG CACCTGTACA 
GACGGTGTAG CGAGCTACAC TTGTAGTTGT GAGAACGGCT GGGAGGGTGA AAACTGTGAT 
CAAAACATAG ACGACTGCTT GACCTCCCCC TGTCTCCACG GGACCTGTAC AGACGGTGTA 
GGAAGCTACA CCTGTAGTTG TGAGAACGGC TGGGAAGGGG ACAACTGTGA TCAGGATATC 
AATGACTGTA GTCCCAACCC CTGTCTCCAC GGCACCTGTA CAGACGGTGT AGCGAGCTAC 
ACTTGTAGTT GTGAGAACGG CTGGGAGGGG AACAACTGTG ATCAAGACAT AGACGATTGT 
GCGGCCTCCC CCTGTCTCCA CGGCACATGT ACAGACGGTG TAGGAAGCTA CACTTGTAGT 
TGTGAGAACG GCTGGGAAGG GGACAGCTGT GATCAAAATA TAGACGACTG CAGTCCTGAC 
CCCTGTGCAC ACGGCACCTG TTATGACGTA GTAGCCGGTT ATACCTGTAC CTGTGAGCAC 
GGTTGGGGCG GAGTCAACTG TAGTAGTAAC ATCGATAATT GTGTTGGCAA CCCCTGTCTC 
CACGGCACCT GTACAGACGG TGTAGGAAGC TACACTTGTA GTTGTGAGAA CGGCTGGGAG 
GGCGAAAACT GTGATAAAAA CATAGACGAC TGCAGTCCTG ACCCCTGTGC ACACGGCACC 
TGTACAGACG GTGTAGGAAG CTACACTTGC AGTTGTGAGA ACGGCTGGGA GGGTGTCAAC 
TGTAATCAAA ATATAGACGA CTGTGCGGCC ACCCCCTGTC TCCACGGCAC CTGTACAGAC 
GGTGTAGCGA GCTACACGTG TAGTTGTGAG AACGGCTGGG ATGGGGTCAA TTGTGATCAA 
AACATAGACG ACTGCAGTCC CAGCCCCTGC CTCCATGGCT CCTGTACAGA CGGTGTAGCG 
AGCTACACTT GTAGTTGTGA CAACGGTTGG GAGGGTGAAA ACTGTGACCA AAACATAGAC 
GACTGTGCAG TTTCACCCTG TTCACACGGC ATCTGTACAG ACGGCGTAGC GAGCTACACT 
TGTAGTTGTG AGAACGGCTG GGAGGGTGTT AACTGTGACC AAAACATAGA CGATTGTGCG 
GCCTCCCCCT GTACACACGG CACCTGTACA GACGGTGTAG CGAGCTACAC TTGTAGTTGT 
GAGAACGGCT GGGAGGGTGA TAACTGTGAT CAAAACATAG ACGACTGTGC GGCCTCCCCC 
TGTCTCCACG GCACCTGTAC AGACGGTGTA GCGAGCTACA CTTGTAGTTG TGAGAACGGC 
TGGGAAGGTT TTAACTGTGA TCAAAATATA GACGACTGCA GTCCTGACCC CTGTGCACAC 
GGCACCTGTA ATGACGTAAT ATCCGGTTAT ACCTGTACCT GTGAGCACGG TTGGGGCGGA 
GTCAACTGTA GTAATAACAT TGATAACTGT GTAGGGTCCC CCTGTCTACA CGGTACGTGC 
ACTGATGGTG TAGCGAGCTA CACGTGTTCT TGTGAGAACG GTTGGGAAGG TGACAACTGT 
GATCAAAATA TAGACGATTG CTCGGCCTCC CCCTGTACAC ACGGCACCTG TACAGACGGT 
GTAGCGAGCT ACACTTGTAG TTGTGAGAAC GGCTGGGAAG GGGACAACTG TGATCAAAAC 
ATAAACGACT GCAGTCCTGA CCCCTGTACA CACGGTATCT GTACAGACGG TGTAGCGAGC 
TACACTTGTA GTTGTGAGAA CGGCTGGGAA GGGGACAACT GTGATCAAAA CATAGACGAC 
TGCGCGGCCT CCCCCTGTAC ACACGGCACC TGTACAGACG GTGTAGCGAG CTACACTTGT 
AGTTGTGAGA ACGGCTGGGA AGGTGATAAC TGTGAAGATC TGTCTACTAC AGAGATTCAT 
TCTACCACGA TGATAGCCAC GACCATGGAA CACACTACCA TGACGACAGC CAAACCAACT 
ACCAACCCGA AGCAGACAAC CGCTCCCAAC CCAACAACAT CTGAACCAAC AACAACAGAA 
AGCTTGGAAA CAACAACGGT CTACCATACC ACGACGGATG AAGAAATCCA CACCTCACTC 
CTTACCTCTA CTTATCTAAC AACTACTGCA CCTCTGACCA CTTCCATACC CTCAACAGAA 
GTTGAAACAA CAGCTTACAC TACCACTGCA GTCACGGAAA CCTACTCTTC TATCTTTGCC 
TCTACTCTCT CATCAACCAC TGCGCCTCTA ACTACCACTG AGGACGTACA TGTGACTACA 
GAGGGTGTAC AGTACACAAC AGCAGACTCT TCCACTTCTT ACTTACAAAC AACAAAATCA 
ACCGAAATGT TCACGGAAGA TGTAAAACCG CAGACAACGC CGGGAGCATT CACGAAGCCG 
TCCGCGACAT TCAAATCCAC TGTACAACAG ACGCAGTCAA GTTTTGTTAC AGCCGAACGT 
CCACCAACCT TTTTCTTCGA AGTCACCATG GTGAACATAG AGTTCACCGA GGAACTCAAC 
AATCCTACAT CTGAAGAGTT TACATCTCTG GCCTCTCAAT TGACAGAAAC GATATGGTTG 
TACCTACAAG AAAGCTATCT AGCAGAAGAC ATCGTCAGTG TGGTGATCAC CAGATTCCGG 
AAGGGGAGTG TCATCGCAGA CTACGAGGTG AACTTGCGTC AGGACACGGT GGACGTTACC 
GATTTACAGG TCCGAGATGT TTTCCTTTTA GCCCTCAGCA ACATCACGAG CAAGAACAAC 
TCACTGGGGA TCCAAGTGGA CTCATTGTGT ATGAAAGATG ACGGGACCAG TACGTGTACG 
GGAGTTAAGG ATTCCTCACA GTGGCCATCT TGGGCTGTTT ACGTCATCGC GGGGGGCGCC 
GCTGCTGTCG TGTTCCTCGC TTTCTTGTTG ACCGTTTGCG TCGTCCTGCG TAGAAAAAAT 
AATACCAGCC TATGTCGCAC ATTCACAAGT GTGAGAGAGG AAAGGGCGAA GGAGGCTACC 
ATCTTCGAGG CAAAGACTTT GGAGTTTGAA GACCATTTGA TCCTGAAGTA CAAAGGGGTC 
AAACTACCGC CAAACTACGT CTGGCTCAGA GACCATTGCA GCGTGCACGC CTGCGGGAAG 
GAAAGCTTCA AAGCTGGTCT ACCCCTCCTA CAAGCTCACC ACCGTGTAGT ATCTGCCTCT 
GTAGCACCTT TACAGATAAG ATTGGTATAC ACTTTCAGGA CAGGAGACAG TATTAACCAC 
GTGACCAAAG AGCGTATGGT GGATTCTGTC ATGATTGACC CCAACATCCA ACCGGTGAAT 
GTCACTGTAA AAGAAGGGAA TCTAAAAATC AAATGGCCCG ATGGTCACCA GTCGGAGTAC 
GGCCTGGACT GGTTGCTTCA CAACACGTAT GAAGGCCAGA AGCACGTTTG TGGGACCATG 
GGCCCCTTTC TGTGGGACGC GGCAACCCTA TCAGCCTCCC CTCCTCCCAG GGTCCAGTAC 
CAGGACTATC TCACAAACGA CAGGCAGCTG GCTAAGGTTC TACACACCCT GCTGAAATAC 
GGACTCGCCT TTATTGAAGA AGCACCTGCA ACAATGGAAG CGACATTGGC TGCGGCGGTG 
AGGATCAGTC ATGTCCGTGA GACGTCCTTT GGCCGACACT GGGTTATAAC CAGTGACCAG 
CGACAGGGCG CAGGCTACAC TACAGAGGCT CTCCCCCTGC ACACGGACAA CACTCACTTT 
AACGAACCGG CAGGGTTGCT GGTGTCCCAC ATGTTGGAAG AAGGAGACTC CGGAGGAACC 
TCTGTACTTG TAGACGGTTT CCACGCTGCA GAGAGACTGA GGCAGGACGA CCCAGAGGGG 
TTTGACGTCC TGTCGTCTGT ACCAGTCCCT CACTTCCTGG AACATTCCTT AGACATTACA 
GGGACTGGAC CAATAGTGGA GCTGGAACCA GGTCCACGTA AGGAGCTCAG GATGATAAGA 
TACAGCGACT ATGTCCGAGG TTCCCTGGAC ACCATACCGA TGGAGGCTGT AGGTCGCTGG 
TACTCCGCAC TGAGGCGCTT CACCACGTAT TTACGGGACC CCGCCAACGC GTACTGGTTC 
CAACTCAAAC CAGGTCAAAT TCTGCTGATG TATAACTGGC GGCTCCTGCA TGGTCGGTCG 
GCTTTCACAG GGAGCCAACG GAAGCTCGGC GGCTGCTACC TACCAAGGGA TGGTTTCTTG 
AGCAGGGCTA GAAGCCTGAA GATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002601380.1
CDS	1..6450
Translation	MTAKTVLFFWLFVVLSKQATACDSAIDAGFTPTGVITSPNYPSTYPKDTQCTWTITALAGQAVTLDITDFNLAGYNCGTGDTLEVFDGSSDATTLLDTYCVGNTPDGQIVSTGPSMTVVFTSLGNNFFSNGFSATYGTTIGQASTEKVGYEAWRGRLNNGKAWQTDTADTNQWLQIDLGSQKFVTGIQTQGLWGGHTKTYKLLYSDDGTTWVTYGDGQDDTIFTGNTDGPTVVQQTLPTPVLTQYVRINPQTFQGTAYIRLRAEILGCDDIDACSPDPCTHGTCTDGVASYTCSCESGWDGQNCDQDIDDCATSPCLHGTCTDGAASYTCSCENGWEGVNCDQDIDDCAASPCTHGTCTDGVASYTCSCENGWEGQNCDQNTYDCSPNPCLHGTCTDGVASYNCSCENGWEGENCDQDIDDCSPNPCLHGTCTDGVASYTCSCESGWEGQSCDQDIDDCAASPCLHGTCTDGVASYTCSCENGWEGVNCDQDIDDCAASPCTHGTCTDGVASYTCSCENGWEGQSCDQNTDDCSPNPCLHGTCTDGVASYTCSCESGWEGQSCDQDIDDCGASPCLHGTCTDGVASYTCSCENGWEGQNCDQDIDDCSPNPCLHGTCTDGVASYTCSCENGWEDIDDCAASPCRHGTCTDGVASYTCSCENGWEGGNCDQDIDDCATSPCTHGTCTDGVASYTCSCENGWGGDNCDQNIDDCSPDPCTHGTCHDAVAGYTCTCEHGWGGVNCSNNIDNCVGNPCLHGTCTDGVASYTCSCENGWEGENCDQNIDDCLTSPCLHGTCTDGVGSYTCSCENGWEGDNCDQDINDCSPNPCLHGTCTDGVASYTCSCENGWEGNNCDQDIDDCAASPCLHGTCTDGVGSYTCSCENGWEGDSCDQNIDDCSPDPCAHGTCYDVVAGYTCTCEHGWGGVNCSSNIDNCVGNPCLHGTCTDGVGSYTCSCENGWEGENCDKNIDDCSPDPCAHGTCTDGVGSYTCSCENGWEGVNCNQNIDDCAATPCLHGTCTDGVASYTCSCENGWDGVNCDQNIDDCSPSPCLHGSCTDGVASYTCSCDNGWEGENCDQNIDDCAVSPCSHGICTDGVASYTCSCENGWEGVNCDQNIDDCAASPCTHGTCTDGVASYTCSCENGWEGDNCDQNIDDCAASPCLHGTCTDGVASYTCSCENGWEGFNCDQNIDDCSPDPCAHGTCNDVISGYTCTCEHGWGGVNCSNNIDNCVGSPCLHGTCTDGVASYTCSCENGWEGDNCDQNIDDCSASPCTHGTCTDGVASYTCSCENGWEGDNCDQNINDCSPDPCTHGICTDGVASYTCSCENGWEGDNCDQNIDDCAASPCTHGTCTDGVASYTCSCENGWEGDNCEDLSTTEIHSTTMIATTMEHTTMTTAKPTTNPKQTTAPNPTTSEPTTTESLETTTVYHTTTDEEIHTSLLTSTYLTTTAPLTTSIPSTEVETTAYTTTAVTETYSSIFASTLSSTTAPLTTTEDVHVTTEGVQYTTADSSTSYLQTTKSTEMFTEDVKPQTTPGAFTKPSATFKSTVQQTQSSFVTAERPPTFFFEVTMVNIEFTEELNNPTSEEFTSLASQLTETIWLYLQESYLAEDIVSVVITRFRKGSVIADYEVNLRQDTVDVTDLQVRDVFLLALSNITSKNNSLGIQVDSLCMKDDGTSTCTGVKDSSQWPSWAVYVIAGGAAAVVFLAFLLTVCVVLRRKNNTSLCRTFTSVREERAKEATIFEAKTLEFEDHLILKYKGVKLPPNYVWLRDHCSVHACGKESFKAGLPLLQAHHRVVSASVAPLQIRLVYTFRTGDSINHVTKERMVDSVMIDPNIQPVNVTVKEGNLKIKWPDGHQSEYGLDWLLHNTYEGQKHVCGTMGPFLWDAATLSASPPPRVQYQDYLTNDRQLAKVLHTLLKYGLAFIEEAPATMEATLAAAVRISHVRETSFGRHWVITSDQRQGAGYTTEALPLHTDNTHFNEPAGLLVSHMLEEGDSGGTSVLVDGFHAAERLRQDDPEGFDVLSSVPVPHFLEHSLDITGTGPIVELEPGPRKELRMIRYSDYVRGSLDTIPMEAVGRWYSALRRFTTYLRDPANAYWFQLKPGQILLMYNWRLLHGRSAFTGSQRKLGGCYLPRDGFLSRARSLKII

Target ORF information:

RefSeq Version	XM_002601334.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002601334.1

ORF Insert Sequence:

ATGACTGCGA AGACAGTTCT CTTCTTTTGG CTCTTCGTGG TGCTCTCAAA ACAAGCCACT 
GCTTGTGACT CCGCCATAGA CGCTGGATTT ACACCTACAG GAGTGATTAC GTCACCAAAC 
TACCCATCCA CGTATCCTAA AGATACACAA TGTACCTGGA CTATTACTGC ACTTGCTGGT 
CAGGCTGTTA CCTTGGACAT AACTGACTTC AACCTAGCTG GTTATAACTG TGGCACTGGG 
GACACACTTG AGGTTTTTGA CGGCAGTTCT GATGCAACGA CTCTACTGGA CACGTATTGT 
GTGGGAAATA CGCCTGATGG ACAGATAGTG TCTACAGGAC CATCAATGAC TGTTGTCTTT 
ACATCGCTCG GAAACAACTT TTTCAGCAAC GGATTCAGTG CCACGTATGG AACTACTATA 
GGACAAGCCT CTACTGAGAA AGTTGGATAC GAGGCTTGGA GGGGTCGTCT GAATAACGGC 
AAGGCGTGGC AGACCGACAC AGCCGATACA AACCAGTGGC TACAGATCGA CCTAGGTAGT 
CAAAAGTTCG TCACCGGCAT CCAAACACAA GGTCTGTGGG GAGGGCACAC CAAGACTTAC 
AAACTGTTGT ACTCTGACGA TGGGACTACG TGGGTCACTT ACGGAGATGG ACAAGATGAC 
ACCATCTTTA CAGGTAACAC CGACGGCCCA ACAGTAGTGC AACAAACCCT ACCAACTCCA 
GTTCTCACAC AGTACGTGAG GATAAATCCA CAAACATTTC AGGGCACCGC TTACATCCGT 
CTGCGGGCAG AGATACTTGG CTGTGATGAT ATAGACGCCT GCAGTCCTGA CCCCTGTACA 
CACGGCACCT GTACAGACGG TGTAGCGAGC TACACTTGTA GTTGTGAGAG CGGCTGGGAT 
GGTCAAAACT GTGACCAAGA TATAGACGAC TGTGCAACCT CCCCCTGTCT ACACGGCACC 
TGTACAGACG GAGCAGCGAG CTACACTTGT AGTTGTGAGA ACGGCTGGGA AGGTGTCAAT 
TGTGATCAAG ATATAGACGA CTGTGCGGCC TCTCCCTGTA CACACGGCAC CTGTACAGAC 
GGTGTAGCGA GCTACACTTG TAGTTGTGAG AACGGCTGGG AAGGTCAAAA CTGTGATCAA 
AATACATACG ACTGCAGTCC CAACCCCTGT CTTCACGGCA CCTGTACAGA CGGTGTAGCG 
AGCTACAATT GTAGTTGTGA GAACGGCTGG GAGGGTGAAA ACTGTGACCA AGACATAGAC 
GACTGCAGTC CCAACCCCTG TCTCCACGGC ACCTGTACAG ACGGTGTAGC GAGCTACACT 
TGTAGTTGTG AGAGCGGCTG GGAAGGTCAA AGCTGTGATC AAGATATAGA CGATTGTGCG 
GCCTCTCCCT GTCTCCACGG CACCTGTACA GACGGTGTAG CGAGCTACAC TTGTAGTTGT 
GAGAACGGCT GGGAAGGTGT CAATTGTGAT CAAGATATAG ACGACTGTGC GGCCTCTCCC 
TGTACACACG GTACCTGTAC AGACGGTGTA GCGAGCTACA CTTGTAGTTG TGAGAACGGC 
TGGGAAGGTC AAAGCTGTGA TCAAAATACA GACGACTGCA GTCCCAACCC CTGTCTCCAC 
GGCACCTGTA CAGACGGTGT AGCGAGCTAC ACTTGTAGTT GTGAGAGCGG CTGGGAAGGT 
CAAAGCTGTG ATCAAGACAT AGACGACTGT GGGGCCTCTC CCTGTCTCCA TGGCACCTGT 
ACAGACGGTG TAGCGAGCTA CACTTGTAGT TGTGAGAACG GCTGGGAAGG TCAAAATTGT 
GATCAAGATA TAGATGACTG CAGTCCCAAC CCCTGTCTCC ACGGCACCTG TACAGACGGT 
GTAGCTAGCT ACACTTGTAG TTGTGAGAAC GGCTGGGAAG ACATAGACGA CTGTGCGGCC 
TCCCCCTGTC GCCACGGCAC CTGTACGGAC GGTGTAGCGA GTTACACTTG TAGTTGTGAG 
AACGGCTGGG AAGGGGGCAA CTGTGATCAG GACATAGACG ACTGTGCGAC CTCTCCCTGT 
ACACACGGTA CCTGTACAGA CGGTGTAGCG AGCTACACTT GTAGTTGTGA GAACGGATGG 
GGAGGGGACA ACTGTGATCA AAATATAGAC GACTGCAGTC CTGACCCCTG TACACACGGC 
ACCTGTCATG ACGCAGTAGC CGGTTATACC TGTACCTGTG AGCACGGTTG GGGCGGAGTC 
AACTGTAGTA ATAATATTGA CAATTGTGTT GGCAACCCCT GCCTCCACGG CACCTGTACA 
GACGGTGTAG CGAGCTACAC TTGTAGTTGT GAGAACGGCT GGGAGGGTGA AAACTGTGAT 
CAAAACATAG ACGACTGCTT GACCTCCCCC TGTCTCCACG GGACCTGTAC AGACGGTGTA 
GGAAGCTACA CCTGTAGTTG TGAGAACGGC TGGGAAGGGG ACAACTGTGA TCAGGATATC 
AATGACTGTA GTCCCAACCC CTGTCTCCAC GGCACCTGTA CAGACGGTGT AGCGAGCTAC 
ACTTGTAGTT GTGAGAACGG CTGGGAGGGG AACAACTGTG ATCAAGACAT AGACGATTGT 
GCGGCCTCCC CCTGTCTCCA CGGCACATGT ACAGACGGTG TAGGAAGCTA CACTTGTAGT 
TGTGAGAACG GCTGGGAAGG GGACAGCTGT GATCAAAATA TAGACGACTG CAGTCCTGAC 
CCCTGTGCAC ACGGCACCTG TTATGACGTA GTAGCCGGTT ATACCTGTAC CTGTGAGCAC 
GGTTGGGGCG GAGTCAACTG TAGTAGTAAC ATCGATAATT GTGTTGGCAA CCCCTGTCTC 
CACGGCACCT GTACAGACGG TGTAGGAAGC TACACTTGTA GTTGTGAGAA CGGCTGGGAG 
GGCGAAAACT GTGATAAAAA CATAGACGAC TGCAGTCCTG ACCCCTGTGC ACACGGCACC 
TGTACAGACG GTGTAGGAAG CTACACTTGC AGTTGTGAGA ACGGCTGGGA GGGTGTCAAC 
TGTAATCAAA ATATAGACGA CTGTGCGGCC ACCCCCTGTC TCCACGGCAC CTGTACAGAC 
GGTGTAGCGA GCTACACGTG TAGTTGTGAG AACGGCTGGG ATGGGGTCAA TTGTGATCAA 
AACATAGACG ACTGCAGTCC CAGCCCCTGC CTCCATGGCT CCTGTACAGA CGGTGTAGCG 
AGCTACACTT GTAGTTGTGA CAACGGTTGG GAGGGTGAAA ACTGTGACCA AAACATAGAC 
GACTGTGCAG TTTCACCCTG TTCACACGGC ATCTGTACAG ACGGCGTAGC GAGCTACACT 
TGTAGTTGTG AGAACGGCTG GGAGGGTGTT AACTGTGACC AAAACATAGA CGATTGTGCG 
GCCTCCCCCT GTACACACGG CACCTGTACA GACGGTGTAG CGAGCTACAC TTGTAGTTGT 
GAGAACGGCT GGGAGGGTGA TAACTGTGAT CAAAACATAG ACGACTGTGC GGCCTCCCCC 
TGTCTCCACG GCACCTGTAC AGACGGTGTA GCGAGCTACA CTTGTAGTTG TGAGAACGGC 
TGGGAAGGTT TTAACTGTGA TCAAAATATA GACGACTGCA GTCCTGACCC CTGTGCACAC 
GGCACCTGTA ATGACGTAAT ATCCGGTTAT ACCTGTACCT GTGAGCACGG TTGGGGCGGA 
GTCAACTGTA GTAATAACAT TGATAACTGT GTAGGGTCCC CCTGTCTACA CGGTACGTGC 
ACTGATGGTG TAGCGAGCTA CACGTGTTCT TGTGAGAACG GTTGGGAAGG TGACAACTGT 
GATCAAAATA TAGACGATTG CTCGGCCTCC CCCTGTACAC ACGGCACCTG TACAGACGGT 
GTAGCGAGCT ACACTTGTAG TTGTGAGAAC GGCTGGGAAG GGGACAACTG TGATCAAAAC 
ATAAACGACT GCAGTCCTGA CCCCTGTACA CACGGTATCT GTACAGACGG TGTAGCGAGC 
TACACTTGTA GTTGTGAGAA CGGCTGGGAA GGGGACAACT GTGATCAAAA CATAGACGAC 
TGCGCGGCCT CCCCCTGTAC ACACGGCACC TGTACAGACG GTGTAGCGAG CTACACTTGT 
AGTTGTGAGA ACGGCTGGGA AGGTGATAAC TGTGAAGATC TGTCTACTAC AGAGATTCAT 
TCTACCACGA TGATAGCCAC GACCATGGAA CACACTACCA TGACGACAGC CAAACCAACT 
ACCAACCCGA AGCAGACAAC CGCTCCCAAC CCAACAACAT CTGAACCAAC AACAACAGAA 
AGCTTGGAAA CAACAACGGT CTACCATACC ACGACGGATG AAGAAATCCA CACCTCACTC 
CTTACCTCTA CTTATCTAAC AACTACTGCA CCTCTGACCA CTTCCATACC CTCAACAGAA 
GTTGAAACAA CAGCTTACAC TACCACTGCA GTCACGGAAA CCTACTCTTC TATCTTTGCC 
TCTACTCTCT CATCAACCAC TGCGCCTCTA ACTACCACTG AGGACGTACA TGTGACTACA 
GAGGGTGTAC AGTACACAAC AGCAGACTCT TCCACTTCTT ACTTACAAAC AACAAAATCA 
ACCGAAATGT TCACGGAAGA TGTAAAACCG CAGACAACGC CGGGAGCATT CACGAAGCCG 
TCCGCGACAT TCAAATCCAC TGTACAACAG ACGCAGTCAA GTTTTGTTAC AGCCGAACGT 
CCACCAACCT TTTTCTTCGA AGTCACCATG GTGAACATAG AGTTCACCGA GGAACTCAAC 
AATCCTACAT CTGAAGAGTT TACATCTCTG GCCTCTCAAT TGACAGAAAC GATATGGTTG 
TACCTACAAG AAAGCTATCT AGCAGAAGAC ATCGTCAGTG TGGTGATCAC CAGATTCCGG 
AAGGGGAGTG TCATCGCAGA CTACGAGGTG AACTTGCGTC AGGACACGGT GGACGTTACC 
GATTTACAGG TCCGAGATGT TTTCCTTTTA GCCCTCAGCA ACATCACGAG CAAGAACAAC 
TCACTGGGGA TCCAAGTGGA CTCATTGTGT ATGAAAGATG ACGGGACCAG TACGTGTACG 
GGAGTTAAGG ATTCCTCACA GTGGCCATCT TGGGCTGTTT ACGTCATCGC GGGGGGCGCC 
GCTGCTGTCG TGTTCCTCGC TTTCTTGTTG ACCGTTTGCG TCGTCCTGCG TAGAAAAAAT 
AATACCAGCC TATGTCGCAC ATTCACAAGT GTGAGAGAGG AAAGGGCGAA GGAGGCTACC 
ATCTTCGAGG CAAAGACTTT GGAGTTTGAA GACCATTTGA TCCTGAAGTA CAAAGGGGTC 
AAACTACCGC CAAACTACGT CTGGCTCAGA GACCATTGCA GCGTGCACGC CTGCGGGAAG 
GAAAGCTTCA AAGCTGGTCT ACCCCTCCTA CAAGCTCACC ACCGTGTAGT ATCTGCCTCT 
GTAGCACCTT TACAGATAAG ATTGGTATAC ACTTTCAGGA CAGGAGACAG TATTAACCAC 
GTGACCAAAG AGCGTATGGT GGATTCTGTC ATGATTGACC CCAACATCCA ACCGGTGAAT 
GTCACTGTAA AAGAAGGGAA TCTAAAAATC AAATGGCCCG ATGGTCACCA GTCGGAGTAC 
GGCCTGGACT GGTTGCTTCA CAACACGTAT GAAGGCCAGA AGCACGTTTG TGGGACCATG 
GGCCCCTTTC TGTGGGACGC GGCAACCCTA TCAGCCTCCC CTCCTCCCAG GGTCCAGTAC 
CAGGACTATC TCACAAACGA CAGGCAGCTG GCTAAGGTTC TACACACCCT GCTGAAATAC 
GGACTCGCCT TTATTGAAGA AGCACCTGCA ACAATGGAAG CGACATTGGC TGCGGCGGTG 
AGGATCAGTC ATGTCCGTGA GACGTCCTTT GGCCGACACT GGGTTATAAC CAGTGACCAG 
CGACAGGGCG CAGGCTACAC TACAGAGGCT CTCCCCCTGC ACACGGACAA CACTCACTTT 
AACGAACCGG CAGGGTTGCT GGTGTCCCAC ATGTTGGAAG AAGGAGACTC CGGAGGAACC 
TCTGTACTTG TAGACGGTTT CCACGCTGCA GAGAGACTGA GGCAGGACGA CCCAGAGGGG 
TTTGACGTCC TGTCGTCTGT ACCAGTCCCT CACTTCCTGG AACATTCCTT AGACATTACA 
GGGACTGGAC CAATAGTGGA GCTGGAACCA GGTCCACGTA AGGAGCTCAG GATGATAAGA 
TACAGCGACT ATGTCCGAGG TTCCCTGGAC ACCATACCGA TGGAGGCTGT AGGTCGCTGG 
TACTCCGCAC TGAGGCGCTT CACCACGTAT TTACGGGACC CCGCCAACGC GTACTGGTTC 
CAACTCAAAC CAGGTCAAAT TCTGCTGATG TATAACTGGC GGCTCCTGCA TGGTCGGTCG 
GCTTTCACAG GGAGCCAACG GAAGCTCGGC GGCTGCTACC TACCAAGGGA TGGTTTCTTG 
AGCAGGGCTA GAAGCCTGAA GATCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_82678 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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