BRAFLDRAFT_118736 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_118736
Entrez Gene ID	7223290
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002613303.1	XP_002613349.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_118736 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_118736 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb08335	XM_002613303.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_118736 ( XM_002613303.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002613349.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb08335
Clone ID Related Accession (Same CDS sequence)	XM_002613303.1
Accession Version	XM_002613303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101565). COMPLETENESS: incomplete on both ends.

ATGAGTGGCA GACCCCGGCA GCAAACCCTT TTCCAAAGCT GGGGGAAGCA GGCCCAACAG 
TCACGGACTG ACCTCAAGTT CAACCATGCT TCAACTTCTA CCAGTGGTGC AGGGGTCATT 
GACTCTGAGG TCATTGACCT TGTGGATGAT ATGGAGGATG ATGAGCTACT CGCTGCAGCG 
ATGGACGGTT ACGTCGAGAC AAGCGATCCT TCGTCGGACG TTCCAACATC TACTGACTGT 
ACGCCTTTAG AGGACCTCCC TGGCTTTGAC CTCTCAGCAG GAAAATTATG GATCTATCCT 
ACAAACTACC CTGTCAGAGA GTACCAGTAT AACATTGTTC ATCAAGCCCT GTTTAAGAAC 
ACTTTGGTAA CCCTGCCAAC GGGACTTGGG AAGACATTCA TCGCGGCTGT AGTAATGTAC 
AACTTCTACA GGTGGTACCC AATGGGGAAG GTCATTTTCA TGGCACCCAC CAAGCCTCTG 
GTGGCCCAAC AGATAGAAGC ATGTTTCAAC ATTATGGGAA TCCCACAGAA CGACACTGCT 
GAAATGACAG GATCCATGCC TCCATCAGAG AGGAAAAGGG CATGGCAGGA GAAGCGTGTG 
TTCTTTCTGA CCCCGCAGGT CATGGTGAAT GACCTAAGCC GAGGGGCGTG CCCGGCAGAT 
GACGTGAAGT GCCTGGTGGT GGATGAGGCT CACAAGGCCC TGGGAAACCA TGCCTTTTGT 
CAGGTTGTAC AAGAGCTAGT CAAATTCACC CTGCAGTTCA GAGTGCTGGC TCTCAGTGCA 
ACACCTGGAG GAGACCTCAA GGGAGTCCAG CAGGTTCTGT CTAACCTGTT AATCACCCAT 
GTGGAGCTGA GATCAGAAGA CTCTGCGGAC ATCCAGCCCT ACACACATGA GAGAAAGGTG 
GAGAAGATCG TGGTGCCACT TGGGGATCAA CTCAAACAGT TACAGGACAG ATATCTGGCA 
GTTGTGGGAG TTTTTGTAGC AAGGCTGGTG AGGAACAAGG CCATGTTCTG TCGGGATCCA 
GCCAAGGTCA CCAAGTTCCA GCTGCTCAAG TCACGTGATC TCTTCAGGGC AAACCCTCCA 
CCTGAAGCAC AGAACCGAGT ACTACAGGGT TGCGTGGAGG GAGACTTTGC CACGTGCATC 
AGCCTGTACC ACGGTTACGA GCTCCTACTC CAACACGGGA TGAAGTCCTT CTACTCGTTC 
CTCGCCAACA TCATGGAGGA CGAGAAGGGA TCCAAGAAGA AAAGAGAGCT GGCTCAGAAC 
CCAGACTTCA TGTTAATGAT GGGGGAACTG AAGGAGATGT TCAACCCTAG TGATGGTAGT 
GCTTCAGTAG GAGGATACGC ACACGGTCAG GAAGCAGCAG GCAGTAGTAC CCAGGCGGCG 
ACAAAACCAG CGGCCACCCG TGTCATGATC TTTGCCCAGT ACCGTGACAG TGTACAGGAG 
ATCGCAGACA TCCTCAACCA TCACCGACCT CTCGTAAGAG TCATGACCTT CATCGGACAG 
GCCTCTACTG GCAAGGTCAA AAAGGGTTTC ACCCAGAAGG AACAGCTGGC TGTGATGAAG 
AAGTTCCGTG AGGGTGGTTA CAACGTGCTG GTGTCCACGT GTGTGGGTGA GGAGGGGCTG 
GATATAGGAG ACGTGGACCT GATCGTGTGT TTCGATGCAC ACAAGAGTCC GATCCGTCTG 
GTGCAGAGGA TGGGTCGTAC AGGCAGGAAG AGACAGGGCA GGATTGTCAT GCTTGTTACT 
GAAGGGAAGG AGGAAAGGAT CTACAACCAG AGCCAGTACA GTAAGAAGAG CATTCACAAG 
GCCTTACAGG GTAGTACCAG ATCTCTGAAC TTCTACATGA ACAACCCCAG GATGGTCCCT 
AGAGGGGTTA ACCCTGCTGT GCACAAGATG CACATCACTG TGGGGAGCTA TCACTGTACA 
GCAAAGGGGA AGGACAGGAG GGGGTCTGTC AAGGGAAGCA AGGCCATCAC TTCTTTCACA 
AAAGCACAAC CAAAAGGTAA AGTTGAAAAG GATGATGGGT TCCTGACAGC ACAGGAGCTA 
GCTGTTTGGA ACGAGAGGTA CAAGCTGTCA GCCGAGGAGG AGAAAGCTGT CCCCATCCTA 
CAACCTGCTC GTTTTGTGCA CTTAAAGGAT ACAGGAACAA GGGACCAGCC CAGAAGACCA 
AGCCTGTCTC TAACTGAGTG GTCTATCTGG CAGAACAGGC TACAGCCCAC TCACCTGATT 
GGACATTCAC AACGGTGCAA GAACTTTGTA GAGCTAATGG AGTTCATTGA CCTCCAAAAG 
GATGCTGGGG ATGAAGACAG CTATGGCATG GAGATGAAGG CGTTCCTCAA TAACGATGAC 
ATCATCACTA CAAGCGGTAC CTCACGCAGC GATATCAGGA CCTTTATTAC AGAGCCTAAA 
ACAACAAAAG AAAGACAGGA AGATGTGGAA AAGAACTCTT CAGTTGGGAA GAGGAGAAGT 
AAGACATTTG AAGGAAATGA AATGCGGCCC ATAGATTCTG ATGATGACTT GCCCGTCATA 
ACACTCAACC CAAACACTGT AAGCAGCACA GCTGTAGGCA CTGGTGACAG TAATGGCAAA 
GGTAGCAAAG CAGGGAGGGC AAAAGATAAG AAGAAGTCAC CAAGAGGTCC TAACAGTCTG 
CCAAAACAGA AGAGAAAACC ATTGGTGGTA GAACTTGCTC ACGATTCAGA CTTTGAATCG 
CAAGAAGGCA AGGAAAATAC CTCTGCCAAA GGTAGCTCAA TTGTGGATAG GAGTTTGCAG 
AGAGAAGCAA AAGTTGTTCA GGAAAACAAA GATAAAACTT TAGACGCTAC ATGTCAAAGC 
GAAAGAGATG ATGACGTAGA GAATGAAGGC TATATGCCAT TTGATGGTGA TGATTGTGCA 
ATACAAGAAG ATACCATGAC TGTCACAGAT GCAACTGATG TGGAAGATGA TGAGGAAGAT 
AAATGGGAAG CAATTTTCCC AACATTTCTA CATAGAAATA AGAGTAACAT TGCAATGCCT 
GTAAAAGAAT CAACAAGACA AAATGGAAGG TATAGAGTAC CAACCCCACC ATCCCTTGAC 
TCTTTTGAAT GGTTGGAGAG ACTGGAGGAA GAAATGGGTT CTTCCATGGA AGACAGTCAA 
GACAATTGCC TCAAACAATC ATTTCCTTGC AAAAAGTCAC TCATCTTTGA AGCTGTCAGC 
AACTATGTTT CTGATGAAAA CAATCCTACG TGTACAGCAC ACAAGACAGA CAATTCAGAT 
TTGCAAGAAG AAAGAGAAAA AGAACAAGAC AATAGTGTTT CACAAAGTAA ACAGATTCTG 
AAACAACATT CAATAGAGGG ATCAAGACAT GTAGAAAATG AAAATACAAT TAGACTAGGT 
GATTTTTCTG GTTCAAAACA AAATAGAGAG GATTCTCATG TGACCAGGAC GAACGACTTG 
CCAGTTCTGC CAAGCAAAAT GCAGAAAGAA ACAAAAACAG TGGATGAGAC AGATATAGAG 
GGAAGTGACA GTGACTCTCT GTTTGGGAAT GGAAGTGATG ACATGTTTGC AGATATGGAA 
ATTGAGGAAG AGACAGCAGA ATGTCCTGAA AAGGAAGAAC AACAAACTAG AACTGTAGAT 
GATCAAGTAC AGGACACAAA TACAGCAGTA GAAGTAGGCA ACCAAGATAT ATCAAATCAC 
AGTGTCTCTC CTGTGTTCGA TACCCGCCAT CCTTTACCTA CCAACTGTAG TCCACCATCT 
TTGTTTTCTG ACTATGAGGA GTCTTTCCAC ACCGAAGTAT CTTCTAAACC AACCAAAAAT 
CAAGACCACA ACAGTGACAA GCCACCAACA ATTCCCATGG TTTGTGCCAA AACGCCTCCA 
TCTCTACAAC AGGTTGTAGC TGATGATAGT CTATTGGGAG TAGATGTAGA TGTTGGGATG 
ACACATACTG ATGGAGAACA GATTCCACTA GAGGAGGAAG GTCCAATTGC CGTGAACTTT 
GACCTGGGCG GGGACAGTCT TTTGGAACCC CAGTTTGACC TGCAGTTCAG CGATGAGGAC 
ATGTTTGCGG ATTCTAGCCC CTCCGGTCAA GTCACAGAAA CAACCAGGGA TTTGATCGGT 
TTTGAAAAAG AAACTTTGTC AGAAGGCTTT CCTCTTCCAA TAAAGACTAA CAGTGGTCCT 
AAGCATGTTG AAGAAGAGGA TGCTTTTAAT TCTATACCCA ACACTCCTCC ATTTCAAAGA 
TACCAGCAGG GTGCCAAGAC AGCAACATAC AACCATCAAG CAAATGACAC TTTTTCTACA 
AACAATGATG ATGAAGCAGA TGGAGTTGGA CATGAAGAAC TTTTCTCAGA ATGTGATATA 
GTAGCCAGTA CCCCTGTTCA TGGCCCTGGA AGGACGAGTA CAAGGGTATC ACTGAGGAGC 
AAGAGAAATG CTACTCCAGA TAACTTGTGT ACTGACGACA GTAGATCTCC AAAGGAACCT 
ACAATACCAA AGAGACATAA TTCCGAAAGT TTGAAGGGAA TCAATAGGAA CACTGTTGTA 
CCGGCTCCAA GTAGAGGATC AAACAAGATT AATTTAGAAA CATGCAATGA TGGACCCACT 
TCTGTTGGCA AAAGCAAACC AGGTGCATCT GCTGCCACCT GGAGCTGTTC CACCTGCACC 
TTCCTGAACC ATGCCCTGTT GTCTGCATGT GAAATGTGTG ACTCTCCCAA ACTAGGTTCA 
AATTCCTTAG ATCCTCCCAA GGCTACTGTT GTCTCCAGTG ACAGTGATGA AGATGATGTT 
GTGGTGAAGA GGAAACGGTC CAGAGCACAG GTGCTCAAGT CACCTGAGAC ATCCACTGCG 
GAAAGGAAGG CTCCTGTGTC CAGTGACAGT GATGAGGATG CAGCTGTAGT GAAGTGGAAA 
CGGTCCAGAG CACAGGTGCT CAACTCACCT GACACATCCA CTGCAGAACA GATAACTCAT 
GTGTCCAGTG ACAGTGATGA GGATGCAGCT GTAGTGAAGA GGAAACGTCC AGAGTCCAGA 
GCACAGGTGC TCAGATCCCC TGATCTATCA GCTGTGGAAG ACCCCACGGA TACGTTCTTA 
GCAAAAGGAA TTGGTAAAGG ACTTAGAAAC AAGGTGCTGA ACACCACAGC AGAAGACTCT 
GACAGTGACT TTGACCTTCC AGCACAGCCA GCGCACAAAT ACAACCAACA GGTCTCAAGG 
CAAGAAAGCA GACAAAAGAA GAATGTAGGC AGGAGAAAAG CTAACTTAAA GAGTCACAGA 
TCTAGACATG CTGCTAGAGG CTTCCTGGAT GAGGAGGCAG AGCTAAGTGA TGAGGAGGCA 
GAGTTTGTGT CATCTGATGA AGATGAGGAG GGGAATGACT CGTTCTTGGA GGGTTTTGTC 
AGCTACTCCT CACAATGCAC ACAGGCTACT TCTGAAGAAG AGATGAGAGC AGTCTACCTG 
CAGTCAGTGC GCAGTCCAGC CCTGCATGCT GGGAGGGGCT ACAAACTGGT CAAGAAACCA 
TGGGACCCAG ATGTCTTCTC TCAGGTCCCT GCCCCAGATG TAGATGACTA TGAACAGGAC 
AGTTTCTGTG TGGGGGATGA TGAGGACATC TCAGCAGCAG AGTGCACCAT GGTGGAACCT 
GTGGAGGAGT CTACATCACC TGACCTGGGT GGGGTTAGAA CCAGGAGACA GAAAAGAGCT 
GCTGTACTAG AGGACACTCC GGAGAGAAAA CAGCAAGGAC AGCCCAAGGG CAAGAGGAAA 
AGAATCGTGA TGGCAGACAC TTCTAGTGAT GAGGACAGTC CTGGTCCAAC TAAACACCAG 
GCTAAGATCC TCACAACTCC TCCTAGGTCT GACAACAAGT CTGAAGAACC CCCCTTCAAG 
ACTCCTGTTG GAAGGCCTCC TGCAAGGAGA TCTCTGATTG GGCAAGCCAG TGGAAGGAGA 
AATATGTCTT TGGGGATATC CAATCAGATG GAAGATGATG ATGATGACTT TGACACAAGT 
AGATACAACA GGAAACTGTG CAATGAGGAC CCAATAGATA GATGTCAGAC TAATTCTTTA 
CCTAGCAGAA ACACAACAAG TGTACAGGGG GATATTGTGT CAACACAAAG ACTTGAAATG 
AGCTTGGCAG AAAGGCTGAG ATTGAAACAG ACTATGGCAG GGAAAAGTAG TAACTTTGAT 
GGTAAACAAA ATACATTTGC ATCAGCTAGT GTGCCTGTGG ACAGAAGAAA AGACTTTGCA 
GCAAAACCAC CTCAAAGATT TGAAGAATCA TTGACCAAGC AAGTGGAATC AGTGAAAGGG 
GTAGAACCTT CCGGTACCAT CACAGATGAC ATGAGAACAA GGAGACCTGA ACCTAGTCCA 
AGAATGATGA ATACTACAAT CACCACACCA AGGTCTGCAT TGTCACGGAA ACTGGAAAAA 
CTGGTGGTGC TGGTGGACTC TCGGGAGATA GCCTCATCAC AGCAGGTAGT GTCCTCCCTG 
CGGTTCCACC ATGACATCAA TGCTGTGGTG TGTCAGCTGG GCAGCTGTGA CTACATCCTC 
AGTAACAGGA TGGGTGTGGA GAGGAAGACT CTCTCAGATT TTTGTAACAG CACCAACAGG 
GAGAAGTTGG TTCAGAGGGT GCAGGAGCTG TGTGAGCTGT TTGACAAGCC CTGCCTCATC 
GTGGAGAAGG ACAGGGTCAA GCCTGGTATG GAACCCAAGA AAGCCAGGAC ACCAAGCAAG 
TACCACAGCA CTGTGCTGGC AGCCCTGGCT CACACCTCTG TCACTGTGCT CTTTACTGAC 
ACCCAAGAAC AAACAGCTTC CTTACTAGCT GACTTGGCCA AATTAGAGCA GAGGAAAGGG 
AACTCTGTAA ATGGAAGTCC TGAGGTGGAA GCAGAAGGGG AACAGGTGTT TCGCATGTAC 
CTGTCCATGC CAGGTGTGCA CTATGCCACA GCCCTTAATT TGTATTACAA CTGTGGTGGA 
CTGGCAGAGC TCATGAAATG CACAGCCATG CAGTTACAGA TGAAGGGAAA GATGTCTCTT 
CAGAAGGCAT CTTCTCTACA CCAATTTCTC AGGCACAACT TCAACCCTCA GATGTTAGTA 
GACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002613349.1
CDS	1..7029
Translation	MSGRPRQQTLFQSWGKQAQQSRTDLKFNHASTSTSGAGVIDSEVIDLVDDMEDDELLAAAMDGYVETSDPSSDVPTSTDCTPLEDLPGFDLSAGKLWIYPTNYPVREYQYNIVHQALFKNTLVTLPTGLGKTFIAAVVMYNFYRWYPMGKVIFMAPTKPLVAQQIEACFNIMGIPQNDTAEMTGSMPPSERKRAWQEKRVFFLTPQVMVNDLSRGACPADDVKCLVVDEAHKALGNHAFCQVVQELVKFTLQFRVLALSATPGGDLKGVQQVLSNLLITHVELRSEDSADIQPYTHERKVEKIVVPLGDQLKQLQDRYLAVVGVFVARLVRNKAMFCRDPAKVTKFQLLKSRDLFRANPPPEAQNRVLQGCVEGDFATCISLYHGYELLLQHGMKSFYSFLANIMEDEKGSKKKRELAQNPDFMLMMGELKEMFNPSDGSASVGGYAHGQEAAGSSTQAATKPAATRVMIFAQYRDSVQEIADILNHHRPLVRVMTFIGQASTGKVKKGFTQKEQLAVMKKFREGGYNVLVSTCVGEEGLDIGDVDLIVCFDAHKSPIRLVQRMGRTGRKRQGRIVMLVTEGKEERIYNQSQYSKKSIHKALQGSTRSLNFYMNNPRMVPRGVNPAVHKMHITVGSYHCTAKGKDRRGSVKGSKAITSFTKAQPKGKVEKDDGFLTAQELAVWNERYKLSAEEEKAVPILQPARFVHLKDTGTRDQPRRPSLSLTEWSIWQNRLQPTHLIGHSQRCKNFVELMEFIDLQKDAGDEDSYGMEMKAFLNNDDIITTSGTSRSDIRTFITEPKTTKERQEDVEKNSSVGKRRSKTFEGNEMRPIDSDDDLPVITLNPNTVSSTAVGTGDSNGKGSKAGRAKDKKKSPRGPNSLPKQKRKPLVVELAHDSDFESQEGKENTSAKGSSIVDRSLQREAKVVQENKDKTLDATCQSERDDDVENEGYMPFDGDDCAIQEDTMTVTDATDVEDDEEDKWEAIFPTFLHRNKSNIAMPVKESTRQNGRYRVPTPPSLDSFEWLERLEEEMGSSMEDSQDNCLKQSFPCKKSLIFEAVSNYVSDENNPTCTAHKTDNSDLQEEREKEQDNSVSQSKQILKQHSIEGSRHVENENTIRLGDFSGSKQNREDSHVTRTNDLPVLPSKMQKETKTVDETDIEGSDSDSLFGNGSDDMFADMEIEEETAECPEKEEQQTRTVDDQVQDTNTAVEVGNQDISNHSVSPVFDTRHPLPTNCSPPSLFSDYEESFHTEVSSKPTKNQDHNSDKPPTIPMVCAKTPPSLQQVVADDSLLGVDVDVGMTHTDGEQIPLEEEGPIAVNFDLGGDSLLEPQFDLQFSDEDMFADSSPSGQVTETTRDLIGFEKETLSEGFPLPIKTNSGPKHVEEEDAFNSIPNTPPFQRYQQGAKTATYNHQANDTFSTNNDDEADGVGHEELFSECDIVASTPVHGPGRTSTRVSLRSKRNATPDNLCTDDSRSPKEPTIPKRHNSESLKGINRNTVVPAPSRGSNKINLETCNDGPTSVGKSKPGASAATWSCSTCTFLNHALLSACEMCDSPKLGSNSLDPPKATVVSSDSDEDDVVVKRKRSRAQVLKSPETSTAERKAPVSSDSDEDAAVVKWKRSRAQVLNSPDTSTAEQITHVSSDSDEDAAVVKRKRPESRAQVLRSPDLSAVEDPTDTFLAKGIGKGLRNKVLNTTAEDSDSDFDLPAQPAHKYNQQVSRQESRQKKNVGRRKANLKSHRSRHAARGFLDEEAELSDEEAEFVSSDEDEEGNDSFLEGFVSYSSQCTQATSEEEMRAVYLQSVRSPALHAGRGYKLVKKPWDPDVFSQVPAPDVDDYEQDSFCVGDDEDISAAECTMVEPVEESTSPDLGGVRTRRQKRAAVLEDTPERKQQGQPKGKRKRIVMADTSSDEDSPGPTKHQAKILTTPPRSDNKSEEPPFKTPVGRPPARRSLIGQASGRRNMSLGISNQMEDDDDDFDTSRYNRKLCNEDPIDRCQTNSLPSRNTTSVQGDIVSTQRLEMSLAERLRLKQTMAGKSSNFDGKQNTFASASVPVDRRKDFAAKPPQRFEESLTKQVESVKGVEPSGTITDDMRTRRPEPSPRMMNTTITTPRSALSRKLEKLVVLVDSREIASSQQVVSSLRFHHDINAVVCQLGSCDYILSNRMGVERKTLSDFCNSTNREKLVQRVQELCELFDKPCLIVEKDRVKPGMEPKKARTPSKYHSTVLAALAHTSVTVLFTDTQEQTASLLADLAKLEQRKGNSVNGSPEVEAEGEQVFRMYLSMPGVHYATALNLYYNCGGLAELMKCTAMQLQMKGKMSLQKASSLHQFLRHNFNPQMLVDK

Target ORF information:

RefSeq Version	XM_002613303.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002613303.1

ORF Insert Sequence:

ATGAGTGGCA GACCCCGGCA GCAAACCCTT TTCCAAAGCT GGGGGAAGCA GGCCCAACAG 
TCACGGACTG ACCTCAAGTT CAACCATGCT TCAACTTCTA CCAGTGGTGC AGGGGTCATT 
GACTCTGAGG TCATTGACCT TGTGGATGAT ATGGAGGATG ATGAGCTACT CGCTGCAGCG 
ATGGACGGTT ACGTCGAGAC AAGCGATCCT TCGTCGGACG TTCCAACATC TACTGACTGT 
ACGCCTTTAG AGGACCTCCC TGGCTTTGAC CTCTCAGCAG GAAAATTATG GATCTATCCT 
ACAAACTACC CTGTCAGAGA GTACCAGTAT AACATTGTTC ATCAAGCCCT GTTTAAGAAC 
ACTTTGGTAA CCCTGCCAAC GGGACTTGGG AAGACATTCA TCGCGGCTGT AGTAATGTAC 
AACTTCTACA GGTGGTACCC AATGGGGAAG GTCATTTTCA TGGCACCCAC CAAGCCTCTG 
GTGGCCCAAC AGATAGAAGC ATGTTTCAAC ATTATGGGAA TCCCACAGAA CGACACTGCT 
GAAATGACAG GATCCATGCC TCCATCAGAG AGGAAAAGGG CATGGCAGGA GAAGCGTGTG 
TTCTTTCTGA CCCCGCAGGT CATGGTGAAT GACCTAAGCC GAGGGGCGTG CCCGGCAGAT 
GACGTGAAGT GCCTGGTGGT GGATGAGGCT CACAAGGCCC TGGGAAACCA TGCCTTTTGT 
CAGGTTGTAC AAGAGCTAGT CAAATTCACC CTGCAGTTCA GAGTGCTGGC TCTCAGTGCA 
ACACCTGGAG GAGACCTCAA GGGAGTCCAG CAGGTTCTGT CTAACCTGTT AATCACCCAT 
GTGGAGCTGA GATCAGAAGA CTCTGCGGAC ATCCAGCCCT ACACACATGA GAGAAAGGTG 
GAGAAGATCG TGGTGCCACT TGGGGATCAA CTCAAACAGT TACAGGACAG ATATCTGGCA 
GTTGTGGGAG TTTTTGTAGC AAGGCTGGTG AGGAACAAGG CCATGTTCTG TCGGGATCCA 
GCCAAGGTCA CCAAGTTCCA GCTGCTCAAG TCACGTGATC TCTTCAGGGC AAACCCTCCA 
CCTGAAGCAC AGAACCGAGT ACTACAGGGT TGCGTGGAGG GAGACTTTGC CACGTGCATC 
AGCCTGTACC ACGGTTACGA GCTCCTACTC CAACACGGGA TGAAGTCCTT CTACTCGTTC 
CTCGCCAACA TCATGGAGGA CGAGAAGGGA TCCAAGAAGA AAAGAGAGCT GGCTCAGAAC 
CCAGACTTCA TGTTAATGAT GGGGGAACTG AAGGAGATGT TCAACCCTAG TGATGGTAGT 
GCTTCAGTAG GAGGATACGC ACACGGTCAG GAAGCAGCAG GCAGTAGTAC CCAGGCGGCG 
ACAAAACCAG CGGCCACCCG TGTCATGATC TTTGCCCAGT ACCGTGACAG TGTACAGGAG 
ATCGCAGACA TCCTCAACCA TCACCGACCT CTCGTAAGAG TCATGACCTT CATCGGACAG 
GCCTCTACTG GCAAGGTCAA AAAGGGTTTC ACCCAGAAGG AACAGCTGGC TGTGATGAAG 
AAGTTCCGTG AGGGTGGTTA CAACGTGCTG GTGTCCACGT GTGTGGGTGA GGAGGGGCTG 
GATATAGGAG ACGTGGACCT GATCGTGTGT TTCGATGCAC ACAAGAGTCC GATCCGTCTG 
GTGCAGAGGA TGGGTCGTAC AGGCAGGAAG AGACAGGGCA GGATTGTCAT GCTTGTTACT 
GAAGGGAAGG AGGAAAGGAT CTACAACCAG AGCCAGTACA GTAAGAAGAG CATTCACAAG 
GCCTTACAGG GTAGTACCAG ATCTCTGAAC TTCTACATGA ACAACCCCAG GATGGTCCCT 
AGAGGGGTTA ACCCTGCTGT GCACAAGATG CACATCACTG TGGGGAGCTA TCACTGTACA 
GCAAAGGGGA AGGACAGGAG GGGGTCTGTC AAGGGAAGCA AGGCCATCAC TTCTTTCACA 
AAAGCACAAC CAAAAGGTAA AGTTGAAAAG GATGATGGGT TCCTGACAGC ACAGGAGCTA 
GCTGTTTGGA ACGAGAGGTA CAAGCTGTCA GCCGAGGAGG AGAAAGCTGT CCCCATCCTA 
CAACCTGCTC GTTTTGTGCA CTTAAAGGAT ACAGGAACAA GGGACCAGCC CAGAAGACCA 
AGCCTGTCTC TAACTGAGTG GTCTATCTGG CAGAACAGGC TACAGCCCAC TCACCTGATT 
GGACATTCAC AACGGTGCAA GAACTTTGTA GAGCTAATGG AGTTCATTGA CCTCCAAAAG 
GATGCTGGGG ATGAAGACAG CTATGGCATG GAGATGAAGG CGTTCCTCAA TAACGATGAC 
ATCATCACTA CAAGCGGTAC CTCACGCAGC GATATCAGGA CCTTTATTAC AGAGCCTAAA 
ACAACAAAAG AAAGACAGGA AGATGTGGAA AAGAACTCTT CAGTTGGGAA GAGGAGAAGT 
AAGACATTTG AAGGAAATGA AATGCGGCCC ATAGATTCTG ATGATGACTT GCCCGTCATA 
ACACTCAACC CAAACACTGT AAGCAGCACA GCTGTAGGCA CTGGTGACAG TAATGGCAAA 
GGTAGCAAAG CAGGGAGGGC AAAAGATAAG AAGAAGTCAC CAAGAGGTCC TAACAGTCTG 
CCAAAACAGA AGAGAAAACC ATTGGTGGTA GAACTTGCTC ACGATTCAGA CTTTGAATCG 
CAAGAAGGCA AGGAAAATAC CTCTGCCAAA GGTAGCTCAA TTGTGGATAG GAGTTTGCAG 
AGAGAAGCAA AAGTTGTTCA GGAAAACAAA GATAAAACTT TAGACGCTAC ATGTCAAAGC 
GAAAGAGATG ATGACGTAGA GAATGAAGGC TATATGCCAT TTGATGGTGA TGATTGTGCA 
ATACAAGAAG ATACCATGAC TGTCACAGAT GCAACTGATG TGGAAGATGA TGAGGAAGAT 
AAATGGGAAG CAATTTTCCC AACATTTCTA CATAGAAATA AGAGTAACAT TGCAATGCCT 
GTAAAAGAAT CAACAAGACA AAATGGAAGG TATAGAGTAC CAACCCCACC ATCCCTTGAC 
TCTTTTGAAT GGTTGGAGAG ACTGGAGGAA GAAATGGGTT CTTCCATGGA AGACAGTCAA 
GACAATTGCC TCAAACAATC ATTTCCTTGC AAAAAGTCAC TCATCTTTGA AGCTGTCAGC 
AACTATGTTT CTGATGAAAA CAATCCTACG TGTACAGCAC ACAAGACAGA CAATTCAGAT 
TTGCAAGAAG AAAGAGAAAA AGAACAAGAC AATAGTGTTT CACAAAGTAA ACAGATTCTG 
AAACAACATT CAATAGAGGG ATCAAGACAT GTAGAAAATG AAAATACAAT TAGACTAGGT 
GATTTTTCTG GTTCAAAACA AAATAGAGAG GATTCTCATG TGACCAGGAC GAACGACTTG 
CCAGTTCTGC CAAGCAAAAT GCAGAAAGAA ACAAAAACAG TGGATGAGAC AGATATAGAG 
GGAAGTGACA GTGACTCTCT GTTTGGGAAT GGAAGTGATG ACATGTTTGC AGATATGGAA 
ATTGAGGAAG AGACAGCAGA ATGTCCTGAA AAGGAAGAAC AACAAACTAG AACTGTAGAT 
GATCAAGTAC AGGACACAAA TACAGCAGTA GAAGTAGGCA ACCAAGATAT ATCAAATCAC 
AGTGTCTCTC CTGTGTTCGA TACCCGCCAT CCTTTACCTA CCAACTGTAG TCCACCATCT 
TTGTTTTCTG ACTATGAGGA GTCTTTCCAC ACCGAAGTAT CTTCTAAACC AACCAAAAAT 
CAAGACCACA ACAGTGACAA GCCACCAACA ATTCCCATGG TTTGTGCCAA AACGCCTCCA 
TCTCTACAAC AGGTTGTAGC TGATGATAGT CTATTGGGAG TAGATGTAGA TGTTGGGATG 
ACACATACTG ATGGAGAACA GATTCCACTA GAGGAGGAAG GTCCAATTGC CGTGAACTTT 
GACCTGGGCG GGGACAGTCT TTTGGAACCC CAGTTTGACC TGCAGTTCAG CGATGAGGAC 
ATGTTTGCGG ATTCTAGCCC CTCCGGTCAA GTCACAGAAA CAACCAGGGA TTTGATCGGT 
TTTGAAAAAG AAACTTTGTC AGAAGGCTTT CCTCTTCCAA TAAAGACTAA CAGTGGTCCT 
AAGCATGTTG AAGAAGAGGA TGCTTTTAAT TCTATACCCA ACACTCCTCC ATTTCAAAGA 
TACCAGCAGG GTGCCAAGAC AGCAACATAC AACCATCAAG CAAATGACAC TTTTTCTACA 
AACAATGATG ATGAAGCAGA TGGAGTTGGA CATGAAGAAC TTTTCTCAGA ATGTGATATA 
GTAGCCAGTA CCCCTGTTCA TGGCCCTGGA AGGACGAGTA CAAGGGTATC ACTGAGGAGC 
AAGAGAAATG CTACTCCAGA TAACTTGTGT ACTGACGACA GTAGATCTCC AAAGGAACCT 
ACAATACCAA AGAGACATAA TTCCGAAAGT TTGAAGGGAA TCAATAGGAA CACTGTTGTA 
CCGGCTCCAA GTAGAGGATC AAACAAGATT AATTTAGAAA CATGCAATGA TGGACCCACT 
TCTGTTGGCA AAAGCAAACC AGGTGCATCT GCTGCCACCT GGAGCTGTTC CACCTGCACC 
TTCCTGAACC ATGCCCTGTT GTCTGCATGT GAAATGTGTG ACTCTCCCAA ACTAGGTTCA 
AATTCCTTAG ATCCTCCCAA GGCTACTGTT GTCTCCAGTG ACAGTGATGA AGATGATGTT 
GTGGTGAAGA GGAAACGGTC CAGAGCACAG GTGCTCAAGT CACCTGAGAC ATCCACTGCG 
GAAAGGAAGG CTCCTGTGTC CAGTGACAGT GATGAGGATG CAGCTGTAGT GAAGTGGAAA 
CGGTCCAGAG CACAGGTGCT CAACTCACCT GACACATCCA CTGCAGAACA GATAACTCAT 
GTGTCCAGTG ACAGTGATGA GGATGCAGCT GTAGTGAAGA GGAAACGTCC AGAGTCCAGA 
GCACAGGTGC TCAGATCCCC TGATCTATCA GCTGTGGAAG ACCCCACGGA TACGTTCTTA 
GCAAAAGGAA TTGGTAAAGG ACTTAGAAAC AAGGTGCTGA ACACCACAGC AGAAGACTCT 
GACAGTGACT TTGACCTTCC AGCACAGCCA GCGCACAAAT ACAACCAACA GGTCTCAAGG 
CAAGAAAGCA GACAAAAGAA GAATGTAGGC AGGAGAAAAG CTAACTTAAA GAGTCACAGA 
TCTAGACATG CTGCTAGAGG CTTCCTGGAT GAGGAGGCAG AGCTAAGTGA TGAGGAGGCA 
GAGTTTGTGT CATCTGATGA AGATGAGGAG GGGAATGACT CGTTCTTGGA GGGTTTTGTC 
AGCTACTCCT CACAATGCAC ACAGGCTACT TCTGAAGAAG AGATGAGAGC AGTCTACCTG 
CAGTCAGTGC GCAGTCCAGC CCTGCATGCT GGGAGGGGCT ACAAACTGGT CAAGAAACCA 
TGGGACCCAG ATGTCTTCTC TCAGGTCCCT GCCCCAGATG TAGATGACTA TGAACAGGAC 
AGTTTCTGTG TGGGGGATGA TGAGGACATC TCAGCAGCAG AGTGCACCAT GGTGGAACCT 
GTGGAGGAGT CTACATCACC TGACCTGGGT GGGGTTAGAA CCAGGAGACA GAAAAGAGCT 
GCTGTACTAG AGGACACTCC GGAGAGAAAA CAGCAAGGAC AGCCCAAGGG CAAGAGGAAA 
AGAATCGTGA TGGCAGACAC TTCTAGTGAT GAGGACAGTC CTGGTCCAAC TAAACACCAG 
GCTAAGATCC TCACAACTCC TCCTAGGTCT GACAACAAGT CTGAAGAACC CCCCTTCAAG 
ACTCCTGTTG GAAGGCCTCC TGCAAGGAGA TCTCTGATTG GGCAAGCCAG TGGAAGGAGA 
AATATGTCTT TGGGGATATC CAATCAGATG GAAGATGATG ATGATGACTT TGACACAAGT 
AGATACAACA GGAAACTGTG CAATGAGGAC CCAATAGATA GATGTCAGAC TAATTCTTTA 
CCTAGCAGAA ACACAACAAG TGTACAGGGG GATATTGTGT CAACACAAAG ACTTGAAATG 
AGCTTGGCAG AAAGGCTGAG ATTGAAACAG ACTATGGCAG GGAAAAGTAG TAACTTTGAT 
GGTAAACAAA ATACATTTGC ATCAGCTAGT GTGCCTGTGG ACAGAAGAAA AGACTTTGCA 
GCAAAACCAC CTCAAAGATT TGAAGAATCA TTGACCAAGC AAGTGGAATC AGTGAAAGGG 
GTAGAACCTT CCGGTACCAT CACAGATGAC ATGAGAACAA GGAGACCTGA ACCTAGTCCA 
AGAATGATGA ATACTACAAT CACCACACCA AGGTCTGCAT TGTCACGGAA ACTGGAAAAA 
CTGGTGGTGC TGGTGGACTC TCGGGAGATA GCCTCATCAC AGCAGGTAGT GTCCTCCCTG 
CGGTTCCACC ATGACATCAA TGCTGTGGTG TGTCAGCTGG GCAGCTGTGA CTACATCCTC 
AGTAACAGGA TGGGTGTGGA GAGGAAGACT CTCTCAGATT TTTGTAACAG CACCAACAGG 
GAGAAGTTGG TTCAGAGGGT GCAGGAGCTG TGTGAGCTGT TTGACAAGCC CTGCCTCATC 
GTGGAGAAGG ACAGGGTCAA GCCTGGTATG GAACCCAAGA AAGCCAGGAC ACCAAGCAAG 
TACCACAGCA CTGTGCTGGC AGCCCTGGCT CACACCTCTG TCACTGTGCT CTTTACTGAC 
ACCCAAGAAC AAACAGCTTC CTTACTAGCT GACTTGGCCA AATTAGAGCA GAGGAAAGGG 
AACTCTGTAA ATGGAAGTCC TGAGGTGGAA GCAGAAGGGG AACAGGTGTT TCGCATGTAC 
CTGTCCATGC CAGGTGTGCA CTATGCCACA GCCCTTAATT TGTATTACAA CTGTGGTGGA 
CTGGCAGAGC TCATGAAATG CACAGCCATG CAGTTACAGA TGAAGGGAAA GATGTCTCTT 
CAGAAGGCAT CTTCTCTACA CCAATTTCTC AGGCACAACT TCAACCCTCA GATGTTAGTA 
GACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_118736 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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