BRAFLDRAFT_105734 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_105734
Entrez Gene ID	7222081
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002586691.1	XP_002586737.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_105734 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_105734 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb07739	XM_002586691.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_105734 ( XM_002586691.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002586737.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb07739
Clone ID Related Accession (Same CDS sequence)	XM_002586691.1
Accession Version	XM_002586691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101309). COMPLETENESS: incomplete on both ends.

ATGTCGCCGC TCGCCGTCTT CTGTCTTTCC TGCGCGAGCC TGGCGTGCTT TGTCGGTCCG 
GTCTCGGCAC AAGTCACTGG AAATTACGAA GACCATTCTG GTGACCAGGG TTACAGCGGT 
TATGGAGAAA CTGGGAGTCC TGCATATTAT AACCCCGAAG ACCATTCTGG TGACTACAGC 
GGTTATGGAG GAGAAACTGG GAGTGCTGAG TATTATAATC CCGACAGTTC AGGAGACTAC 
GGGTACGGAA GCGGTTCTGG GGACGCGATG ACAGGGACTG GTGTATATTA CGAGCCAATG 
TACTACGGTC TGGACCCCAA CGGTACTTGC AGCATCTCCC AGTGTCAGAA CAGCGCTGCA 
TGCTGCGAGG CCCCCGAAGG AGACTGGTAC TGTTACTGCT TTCTTGGGTA CGAGGAACCT 
GACTGCAGCT ACTTCAGTGA TCCATGCATG TCAGAAGAGA ATCCTTGCAT GAACGAAGGC 
ACCTGTCATT TCGACTCCTA CAATCACCAA GCCTACTGCG AGTGTCCGCC AGGATTCACA 
GGACGAGCGT GCGAGTTTTC TATGCCCATC GACTGTACCT CAGACGAGTG TCAGAACGGA 
GGGACCTGTA TAATGGACTC GTGGGAAGGT CCCTATTGTA GCTGTCCGGA CGGTTTCTCG 
GGGCCTGTGT GCGAGTTTGT GATAGGGCAA TGCGAGGAAG ACACCTGCGT GAACGGCCAC 
TGCGTCGGAT GGATCTGCGC GGGATACTAC GCCTGTAACT GTGACGAAGG ATACTACGGG 
ACGTTCTGTG AAATACCAGA TGATGGTCAG TACAATGATG GCGAGGGACC AACTGGGTCT 
GAAGACTCCG CTGCAGATGC TTGCAGCAGC TCCACCTGTC CAGACGGCGT TTCCTGCTGT 
GTGGACCCAG AGGGAGGCTG GTACTGTGAC TGTCCGCTGG GTTACTATGG TCCGACATGT 
GCCGACTATA CCGACCCCTG CGCTGGAAAC AACCCCTGCC AGCACGGAGG CAACTGCACG 
GCGCCATACA GTTTCTCCGC TGACGCCTAC TGCTCGTGTC CAAGAGGATT CACAGGATAT 
TCCGGGACAT TCTGCGAAGT AGAAGGTGAT GGTGGTAACA ATGGGCCAAC CGGTTCTGGA 
GACTATGGCC CTGGTGATGG TCAGTACAAC GATACCGGTC CATGCAGCAG CTCCCCCTGT 
GAAAACGGCG CCTCCTGCTG TGAGGATCCG GAAGGAGGCT GGTACTGTGA CTGTCCGCTG 
GGATACGATC CTCCGACATG TGCCACCTTT ACCAACCCCT GCGTAGACGA GAACCCCTGC 
ATGGAGGGAG GCACCTGTGT CTTCGACCCA ACCATGCCTG ACTACGCCTA TTGCCAGTGT 
CCGCCAGGAT TCACAGGTAA AGTGTGTGAG ACCACCGTAC CCACCGACTG TGCCTTGGGA 
GAATGTCAGA ACGGGGGAGC CTGTGAATCG GACTCGTGGG GAGGTAATTA CGTCTGGTGT 
GAATGCCCAG AAGGTTTCAC GGGACCGTAC TGTGAGTTTG CGGTTGGAGA ATGTCAAGAA 
GACAGCTGTG AGCACGGCTC CTGTTATCCG GCAGACTGTG GTGGATACTC CTATATGTGC 
CACTGTGACG GAGGATATAC CGGGACATTC TGTGAAGTAG AAGGTGATGG TCAGCACAGT 
GGTGACTATG AACCAACCGG TTCCGGAGAC TATGGACCGT ACGGCTCTGG AGACTATGGA 
CCAACCGGTT CCGGAGACTA TGGACCGTAC GGCTCTGGAG ACTATGGACC AAGCGGTTCT 
GGGGACTATG GACCTGGTGG CCCTGGAGAC TACCCCATGT ACTACGGCTT GGATCCAAAT 
GGCACTTGCA GCAATTCCCC TTGTCAGAAC AGCGCTGGCT GCTGCGAGGC CCCCGAAGGA 
GACTGGTACT GTTACTGCTT CCTTGGGTAC GAGGAGCCTG ACTGCAGCTA CTTCAGTGAT 
CCCTGTATGT CAGAAGAGAA TCCCTGCATG AACGAAGGCA CCTGTCATTT CGACTCCTAC 
AGTAACCAAG CCTACTGCCA GTGTCCACCA GGATTCACAG GACGAGCGTG TGAGTTTCCA 
ATGCCCACCG ACTGTACCTC AGACGAGTGT CAGAACGGAG GAACCTGTAT CATGGAGTCG 
TGGGGAGATC CCTGGTGTAG CTGTCCGGAC GGTTTCTCGG GGCCTGTGTG CGAGTTTGCG 
ATAGGGCAAT GCGAGGATGA CACCTGCGTG AACGGCGACT GTGTGGAATG GATCTGTGCG 
GGTTACTACG CCTGTAACTG TGATGAAGGT TATTCAGGAA CATTCTGCGA GTACGACGAT 
GACGATGACC AGTCCAGCGG GTCTGGAGAC TATGGACCAG GTGATGGTCA TTATAACGAT 
ACCGATGGTT GCAGCAGCTC CCCCTGTGAA AACGGCGCCT CCTGCTGTGA GGATCCGGAA 
GGAGGCTGGT ACTGTGACTG TCCGCTGGGC TTCCATGGTC CGACATGTGC CTTATTTACC 
AACCCCTGCG TAGACGAAAA CCCCTGCATG GAGGGAGGCA CCTGTGTACC GCCTGACAGT 
TTCTCCCCTG ACTACGCCTA CTGCCTCTGT CCAAGAGGAT TCACAGGTAA AGTGTGTGAG 
ACTACCGTAC CCACCGACTG TACCTTGGGA GAATGTCAGA ACGGAGCAGC CTGTGTAACG 
GACTCTTGGG GAGGTAATGA TGTCTGGTGT GAATGTCTGG AAGGTTTCAC GGGGCCGTAC 
TGTGAGTTTG CGGTTGGAGA ATGTCAAGAA GACAGCTGTG AGCACGGCTC TTGTTATCCG 
GCAGACTGTG GTGGATACTC CTACATGTGC CACTGTGAAG AAGGTACCTG CAGCAACTCC 
CCTTGCCAGA ACAGCGCTGG CTGCTGCGAG GCCCCCGAAG GAGACTGGTA CTGTTACTGC 
CTGCTAGGGT ACGAGGAACC TGGCTGTACA GACTTTAGCG ACCCCTGCAA CTCAGCCTCA 
GGAGAGAACC CCTGCATGCA CGAAGGCACC TGTTATTTCG ACTCCTACAG TAACCAAGCC 
TACTGCCAGT GTCCACCAGG ATTCACAGGG CGAGCGTGCG AGTTTTCTAT GCCCATCGAC 
TGTAACTCAG ACGAGTGTCA GAACGGAGGG ACCTGTATCA TGGAGTCGTG GGGAGATCCC 
TGGTGTAGCT GTCCGGACGG TTTCTCGGGG CCTGTGTGCG AGTTTGCGAT AGGGCAATGC 
GAGGAAGACA CCTGCGTGAA CGGCGACTGC GTAGAATGGA TCTGTGCAGG ATACTACGCC 
TGTAACTGTG ACGAAGGATA TACCGGGACA TTCTGTGAAG TAGAAGATGA TGGTCAGCAC 
AGTGGTGACT ATGGACCAAC CGGGTCTGGA GACTATGGAC CGTACGGCTC TGGAGACTAT 
GGACCGTACG GCTCTGGAGA CTATGGACCT AGCGGTTCTG GGGACTATGG ACCTGGTGAT 
GGTCATTATA ACGATACCGA TGGTTGCAGC AGCTCCCCCT GTGAAAACGG CGCCTCCTGC 
TGTGAGGATC AGGAAGGAGG CTGGTACTGT GACTGTCCGC TGGGGTTCGA TCCTCCGACA 
TGTGCCTACT TTACCAACCC CTGCGTAGAC GAAAACCCCT GCATGGAGGG AGGCACCTGT 
GTACCGCCTG ACAGTTTCTC CCCTGACTAC GCCTACTGCC TCTGTCCAAG AGGATTCACA 
GGTAAAGTGT GTGAGACTAC CGTACCCACC GACTGTACCT TGGGAGAATG TCAGAACGGG 
GGAGCCTGTG AAACGGACTC GTGGGGAGGT GGTGACGTCT GGTGTGACTG CCCAGAAGGT 
TTCACGGGGC CTTACTGTGA GTTTGCGGTT GGAGAATGTC AAGACGACAG CTGTGAGCAC 
GGCTCCTGTT ATCCGGCAGA TTGCGAAGGA TACACTTACA TGTGCCACTG TGAGGAAGGA 
TATTCCGGGA CATTCTGTGA AGTAGAAGGT GATGGTCAGC ACAGTGGTGA CTATGGACCA 
ACTGGGTCCG GAGACTATGG AGCAAGCGGT TCTGGGGACC ATGGACCGTA CGGCTCTGGA 
GACTATGGGC CTAGTGGTTC TGGGGACTAT GGACCTGGAG ACTACGCCGT GTACTACGGC 
TTGGACCCAA ATGGCACTTG TAGCAATTCC CCTTGTCAGA ACAGCGCTGG CTGCTGCGAG 
GCCCCCGAAG GAGACTGGTA CTGCTACTGC CTGCTTGGGT ACGAGGAGCC TGACTGCAGC 
TCCTTTAGTG ATCCCTGCAT GTCAGAAGAG AATCCTTGCA TGAACGAAGG CACCTGTCAT 
TTCGATTCGT ACAGTCGCCA AGCCTACTGC CAGTGTCAAC CAGGATTCAC AGGACGAGCG 
TGTGAGTTTC CAATGCCCAC CGACTGTACC TCAGACGAGT GTCAGAACGG AGGAACCTGT 
ATCATGGAGT CGTGGGGAGA TCCCTGGTGT AGCTGTCCGG ACGGTTTCTC GGGGCCTGTG 
TGCGAGTTTG CGATAGGGCA ATGCGAGGAT GACACCTGCG TGAACGGCCA CTGCGTCGAA 
TGGATCTGTG CGGGATACTA CGCCTGTAAC TGTGACGAAG GATATTCCGG GACATTCTGT 
GAAGTTGAAG GTGATGGCAG TGACTATGGA CCAAGCGGTT CGGGAGACTA TGGACCAACC 
GGGTCTGGGG ACTATGGACC AACCGGGTCC GGAGACTATG TATCACACGG TTCTGGGGAC 
TATGGACCAA ACGGTTCTGG GGACTATGGA CCAAACGGTT CTGGGGACTA TGGACCAAAC 
GGTTCTGGGG ACTATGGACC AAGTGGTTCG GGAGACTATG GACCAACCGG GTCTGGGGAC 
TATGGACCAA CCGGGTCCGG AGACTATGGA TCACACGGTT CTGGGGACTA TGGACCAAAC 
GGTTCTGGGG ACTATGGACC AAACGGTTCT GGGGACTATG GACCAAACGG TTCTGGGGAC 
TATGGACCAA CCGGGTCTGG GGACTATGGA CCAAACGGCT CTGGAGACTA TGGATCACAC 
GGTTCTGGGG ACTATGGACC AAACGGCTCT GGAGACTATG GACCAACCGG GTCTGGGGAC 
TATGGACCAA CCGGGTCTGG GGACTATGGA CCAACCGGGT CCGGGGACTA TGGACCAAAC 
GGTTCTGGGG ACTATGGACC AACCGGGTCC GGTGACTATG GACCATATTA TCCATCTGAG 
GGTCCAGGAC CATATCCATC TGAGGGTCCA GGACCATATC CATCTGCGAG CCCCGAACCT 
TATTATCCAT CTGAGGGTCC AGGACCATAT CCATCTGAGG GTCCAGGACC ACATTATCCA 
TCTGAGGGTC CAGGACCATA TCCATCTGAG GGTCCAGGAC CATATCCATC TGCGAGCCCC 
GAACCTTATT ATCCATCTGA GGGTCCAGGA CCATATCCAT CTGAGGGTCC AGGACCACAT 
TATCCATCTG AGGGTCCAGG ACCATATCCA TCTGAGGGTC CAGGACCATA TCCATCTGAG 
AGCCCCGGAC CATATTATCC ATCTGAGGGT CCAGGGCCAT TTCCATCTGA GGGCCCTGGA 
CCATATTATC CATCTGAGGG TCCAGGGCCA TTTCCGTCTG AGGACCCTCG ACCAATTAAC 
CATACCGAGG GTTGCAGCAG CTCCCCCTGT GAAAACGGCG CCTCCTGCTG TGAGGATCCG 
GAAGGAGGCT GGTACTGTGA CTGTCCGCTG GGGTTCGATC CTCCGACATG TGCCACCTTT 
ACCAACCCCT GCGCTGGGGA AAACCCCTGC ATGAAGGGAG GCACTTGTGT ACCGCCAGAC 
ATTTTCTCCC CTGACTACGC CTACTGCCTC TGTCCAAGAG GATTCACAGG TAAAGTGTGT 
GAGACCACCG TACCAACCGA CTGTGCTTTA AACGAATGTC AGAACGGGGC AGCATGTCAC 
ACGGACTCCT GGGGAGGTGG TGACGTCTGG TGTGACTGCC CAGAAGGTTT CACGGGGCCT 
TACTGTGAGT TTGCGGTTGG AGAATGCCAA ATTGACAGCT GTGAGCACGG CTCCTGTTAC 
CCGGCAGACT GTGATGGATA CTCCTACATG TGCGACTGTG AGGAAGGATA CTCCGGGACA 
TTCTGTGACG TAGAAGGTGA TGGTGGTGAC TATGGACCAA CTGGGTCTGG AGAATATGGA 
CCGAGTGGTT CTGGGGACTA TGGGCCATAT TATCCATCTG AGGGCCCTGG ACCATATCCA 
TCTGAGGGTC CAGGACCACA TCCCTCTGAG GACCCTCGAC CATATAACCC CACTGACGAC 
CCTGGATCAT ATACTACCGA GGTCCCCGGG CCAAATCCGC TTGAAGACTC CAACCTGTAC 
CCGTTCGGTC CGGACGCTGG TGATTTGGAG TATGAGGACC TCCTGAACAG TGGCGACGGC 
CATTATTGGG GAGAGTGTTT GGAGCAGAGT ATCAACGATG AGGGCTTCCC GTTCTATGGA 
AAGAGGCACT ACAAACTCTA TGTTTGTGAC AATGGCATGA TCTCCTTCGA GACGCCATGG 
GCGCCATGGG TGCCGTATCT GTTCGGCTCC CGTCGCATGT ACAGCGACAT CGCCATGATC 
GCGCCGTTCT GGGCCATGTC AGACGCGTCC AGCCTCTCCC AGGACAGGCA GGACACCGAG 
CATTCGCACG TCTACTTCCA CTCGTACGCC GAAGGAGACG GGCAGGCAAA CACCGAGGAG 
ATCCTGAATC GCGCAGGAGA AGATGCGAAC GACTACGATG CATCCATACC AGATTTCAGG 
CCAACATGGG CCCTAGTGGT GACATGGGTC CGCCTTCCCC CGTACATCGG CTGCAGCTGT 
TACCGGGGCT GGGGCTGCCA GGAGTGTGAC CTCACTGACG TACAGACGAA CACGTTCCAG 
GCCGTGCTGA TGACAAACGC CCTGCATTCC TTCGGCAAGT CTATCTACCC GCAGGGAGGG 
CTCAACTGGG TTTATCCAGG ATTTAGCGAT GAGTACTACC AGTACTTCAC GGATGTCCAG 
GACCTACCAT GGCCCGTGGC AGGCTTCAGT GCCGGGGACG TGGGACAGAA CGTCATCAAC 
CTCCCAACGT ACTCAGGAAC CCAGCGGATG CACCTACTGG ATGAGGTCGA GGGGAACACA 
GGCTACGTTG GTCAGTGGAT GGTGCGGATG GAGGACTCGG ACGGCACCAT GGACGCGCGG 
CTGGAGTGTC TGGCCTGGCA CAGGGACCAG CCGGACCCCG CGGACGTGCA GGCCAGTCTG 
AGTGAGCTGC CGCCGGACTG CCCCTGCACG GAGTGGCAGC TGTGGTTTGA TGGAGCGTTC 
CAGATCGACT GGAGCAGCTG GGGCTGGGAT ATCTGCGCCT ACAGCGCCTT CCCCACAGCG 
GACGGGCTGG TGCAGAAATG CTGCTATGAA GACGGCGCGC TGAAGACCGG AGGTCCGGAT 
GGGGGATACG TGACTCAGAA CCGTGCAGAT GACGAGTTGG CATACGCAAA ATGCTGCGTG 
GAAAGTGTGG GGACCTGTGA TCTGTTCTAC GAGAAGCGTC CATCGGCTAA CTGCTCTCAG 
TACCTCCCAC CCATATGGAG TAGGTGTATT TTATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002586737.1
CDS	1..7599
Translation	MSPLAVFCLSCASLACFVGPVSAQVTGNYEDHSGDQGYSGYGETGSPAYYNPEDHSGDYSGYGGETGSAEYYNPDSSGDYGYGSGSGDAMTGTGVYYEPMYYGLDPNGTCSISQCQNSAACCEAPEGDWYCYCFLGYEEPDCSYFSDPCMSEENPCMNEGTCHFDSYNHQAYCECPPGFTGRACEFSMPIDCTSDECQNGGTCIMDSWEGPYCSCPDGFSGPVCEFVIGQCEEDTCVNGHCVGWICAGYYACNCDEGYYGTFCEIPDDGQYNDGEGPTGSEDSAADACSSSTCPDGVSCCVDPEGGWYCDCPLGYYGPTCADYTDPCAGNNPCQHGGNCTAPYSFSADAYCSCPRGFTGYSGTFCEVEGDGGNNGPTGSGDYGPGDGQYNDTGPCSSSPCENGASCCEDPEGGWYCDCPLGYDPPTCATFTNPCVDENPCMEGGTCVFDPTMPDYAYCQCPPGFTGKVCETTVPTDCALGECQNGGACESDSWGGNYVWCECPEGFTGPYCEFAVGECQEDSCEHGSCYPADCGGYSYMCHCDGGYTGTFCEVEGDGQHSGDYEPTGSGDYGPYGSGDYGPTGSGDYGPYGSGDYGPSGSGDYGPGGPGDYPMYYGLDPNGTCSNSPCQNSAGCCEAPEGDWYCYCFLGYEEPDCSYFSDPCMSEENPCMNEGTCHFDSYSNQAYCQCPPGFTGRACEFPMPTDCTSDECQNGGTCIMESWGDPWCSCPDGFSGPVCEFAIGQCEDDTCVNGDCVEWICAGYYACNCDEGYSGTFCEYDDDDDQSSGSGDYGPGDGHYNDTDGCSSSPCENGASCCEDPEGGWYCDCPLGFHGPTCALFTNPCVDENPCMEGGTCVPPDSFSPDYAYCLCPRGFTGKVCETTVPTDCTLGECQNGAACVTDSWGGNDVWCECLEGFTGPYCEFAVGECQEDSCEHGSCYPADCGGYSYMCHCEEGTCSNSPCQNSAGCCEAPEGDWYCYCLLGYEEPGCTDFSDPCNSASGENPCMHEGTCYFDSYSNQAYCQCPPGFTGRACEFSMPIDCNSDECQNGGTCIMESWGDPWCSCPDGFSGPVCEFAIGQCEEDTCVNGDCVEWICAGYYACNCDEGYTGTFCEVEDDGQHSGDYGPTGSGDYGPYGSGDYGPYGSGDYGPSGSGDYGPGDGHYNDTDGCSSSPCENGASCCEDQEGGWYCDCPLGFDPPTCAYFTNPCVDENPCMEGGTCVPPDSFSPDYAYCLCPRGFTGKVCETTVPTDCTLGECQNGGACETDSWGGGDVWCDCPEGFTGPYCEFAVGECQDDSCEHGSCYPADCEGYTYMCHCEEGYSGTFCEVEGDGQHSGDYGPTGSGDYGASGSGDHGPYGSGDYGPSGSGDYGPGDYAVYYGLDPNGTCSNSPCQNSAGCCEAPEGDWYCYCLLGYEEPDCSSFSDPCMSEENPCMNEGTCHFDSYSRQAYCQCQPGFTGRACEFPMPTDCTSDECQNGGTCIMESWGDPWCSCPDGFSGPVCEFAIGQCEDDTCVNGHCVEWICAGYYACNCDEGYSGTFCEVEGDGSDYGPSGSGDYGPTGSGDYGPTGSGDYVSHGSGDYGPNGSGDYGPNGSGDYGPNGSGDYGPSGSGDYGPTGSGDYGPTGSGDYGSHGSGDYGPNGSGDYGPNGSGDYGPNGSGDYGPTGSGDYGPNGSGDYGSHGSGDYGPNGSGDYGPTGSGDYGPTGSGDYGPTGSGDYGPNGSGDYGPTGSGDYGPYYPSEGPGPYPSEGPGPYPSASPEPYYPSEGPGPYPSEGPGPHYPSEGPGPYPSEGPGPYPSASPEPYYPSEGPGPYPSEGPGPHYPSEGPGPYPSEGPGPYPSESPGPYYPSEGPGPFPSEGPGPYYPSEGPGPFPSEDPRPINHTEGCSSSPCENGASCCEDPEGGWYCDCPLGFDPPTCATFTNPCAGENPCMKGGTCVPPDIFSPDYAYCLCPRGFTGKVCETTVPTDCALNECQNGAACHTDSWGGGDVWCDCPEGFTGPYCEFAVGECQIDSCEHGSCYPADCDGYSYMCDCEEGYSGTFCDVEGDGGDYGPTGSGEYGPSGSGDYGPYYPSEGPGPYPSEGPGPHPSEDPRPYNPTDDPGSYTTEVPGPNPLEDSNLYPFGPDAGDLEYEDLLNSGDGHYWGECLEQSINDEGFPFYGKRHYKLYVCDNGMISFETPWAPWVPYLFGSRRMYSDIAMIAPFWAMSDASSLSQDRQDTEHSHVYFHSYAEGDGQANTEEILNRAGEDANDYDASIPDFRPTWALVVTWVRLPPYIGCSCYRGWGCQECDLTDVQTNTFQAVLMTNALHSFGKSIYPQGGLNWVYPGFSDEYYQYFTDVQDLPWPVAGFSAGDVGQNVINLPTYSGTQRMHLLDEVEGNTGYVGQWMVRMEDSDGTMDARLECLAWHRDQPDPADVQASLSELPPDCPCTEWQLWFDGAFQIDWSSWGWDICAYSAFPTADGLVQKCCYEDGALKTGGPDGGYVTQNRADDELAYAKCCVESVGTCDLFYEKRPSANCSQYLPPIWSRCILL

Target ORF information:

RefSeq Version	XM_002586691.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002586691.1

ORF Insert Sequence:

ATGTCGCCGC TCGCCGTCTT CTGTCTTTCC TGCGCGAGCC TGGCGTGCTT TGTCGGTCCG 
GTCTCGGCAC AAGTCACTGG AAATTACGAA GACCATTCTG GTGACCAGGG TTACAGCGGT 
TATGGAGAAA CTGGGAGTCC TGCATATTAT AACCCCGAAG ACCATTCTGG TGACTACAGC 
GGTTATGGAG GAGAAACTGG GAGTGCTGAG TATTATAATC CCGACAGTTC AGGAGACTAC 
GGGTACGGAA GCGGTTCTGG GGACGCGATG ACAGGGACTG GTGTATATTA CGAGCCAATG 
TACTACGGTC TGGACCCCAA CGGTACTTGC AGCATCTCCC AGTGTCAGAA CAGCGCTGCA 
TGCTGCGAGG CCCCCGAAGG AGACTGGTAC TGTTACTGCT TTCTTGGGTA CGAGGAACCT 
GACTGCAGCT ACTTCAGTGA TCCATGCATG TCAGAAGAGA ATCCTTGCAT GAACGAAGGC 
ACCTGTCATT TCGACTCCTA CAATCACCAA GCCTACTGCG AGTGTCCGCC AGGATTCACA 
GGACGAGCGT GCGAGTTTTC TATGCCCATC GACTGTACCT CAGACGAGTG TCAGAACGGA 
GGGACCTGTA TAATGGACTC GTGGGAAGGT CCCTATTGTA GCTGTCCGGA CGGTTTCTCG 
GGGCCTGTGT GCGAGTTTGT GATAGGGCAA TGCGAGGAAG ACACCTGCGT GAACGGCCAC 
TGCGTCGGAT GGATCTGCGC GGGATACTAC GCCTGTAACT GTGACGAAGG ATACTACGGG 
ACGTTCTGTG AAATACCAGA TGATGGTCAG TACAATGATG GCGAGGGACC AACTGGGTCT 
GAAGACTCCG CTGCAGATGC TTGCAGCAGC TCCACCTGTC CAGACGGCGT TTCCTGCTGT 
GTGGACCCAG AGGGAGGCTG GTACTGTGAC TGTCCGCTGG GTTACTATGG TCCGACATGT 
GCCGACTATA CCGACCCCTG CGCTGGAAAC AACCCCTGCC AGCACGGAGG CAACTGCACG 
GCGCCATACA GTTTCTCCGC TGACGCCTAC TGCTCGTGTC CAAGAGGATT CACAGGATAT 
TCCGGGACAT TCTGCGAAGT AGAAGGTGAT GGTGGTAACA ATGGGCCAAC CGGTTCTGGA 
GACTATGGCC CTGGTGATGG TCAGTACAAC GATACCGGTC CATGCAGCAG CTCCCCCTGT 
GAAAACGGCG CCTCCTGCTG TGAGGATCCG GAAGGAGGCT GGTACTGTGA CTGTCCGCTG 
GGATACGATC CTCCGACATG TGCCACCTTT ACCAACCCCT GCGTAGACGA GAACCCCTGC 
ATGGAGGGAG GCACCTGTGT CTTCGACCCA ACCATGCCTG ACTACGCCTA TTGCCAGTGT 
CCGCCAGGAT TCACAGGTAA AGTGTGTGAG ACCACCGTAC CCACCGACTG TGCCTTGGGA 
GAATGTCAGA ACGGGGGAGC CTGTGAATCG GACTCGTGGG GAGGTAATTA CGTCTGGTGT 
GAATGCCCAG AAGGTTTCAC GGGACCGTAC TGTGAGTTTG CGGTTGGAGA ATGTCAAGAA 
GACAGCTGTG AGCACGGCTC CTGTTATCCG GCAGACTGTG GTGGATACTC CTATATGTGC 
CACTGTGACG GAGGATATAC CGGGACATTC TGTGAAGTAG AAGGTGATGG TCAGCACAGT 
GGTGACTATG AACCAACCGG TTCCGGAGAC TATGGACCGT ACGGCTCTGG AGACTATGGA 
CCAACCGGTT CCGGAGACTA TGGACCGTAC GGCTCTGGAG ACTATGGACC AAGCGGTTCT 
GGGGACTATG GACCTGGTGG CCCTGGAGAC TACCCCATGT ACTACGGCTT GGATCCAAAT 
GGCACTTGCA GCAATTCCCC TTGTCAGAAC AGCGCTGGCT GCTGCGAGGC CCCCGAAGGA 
GACTGGTACT GTTACTGCTT CCTTGGGTAC GAGGAGCCTG ACTGCAGCTA CTTCAGTGAT 
CCCTGTATGT CAGAAGAGAA TCCCTGCATG AACGAAGGCA CCTGTCATTT CGACTCCTAC 
AGTAACCAAG CCTACTGCCA GTGTCCACCA GGATTCACAG GACGAGCGTG TGAGTTTCCA 
ATGCCCACCG ACTGTACCTC AGACGAGTGT CAGAACGGAG GAACCTGTAT CATGGAGTCG 
TGGGGAGATC CCTGGTGTAG CTGTCCGGAC GGTTTCTCGG GGCCTGTGTG CGAGTTTGCG 
ATAGGGCAAT GCGAGGATGA CACCTGCGTG AACGGCGACT GTGTGGAATG GATCTGTGCG 
GGTTACTACG CCTGTAACTG TGATGAAGGT TATTCAGGAA CATTCTGCGA GTACGACGAT 
GACGATGACC AGTCCAGCGG GTCTGGAGAC TATGGACCAG GTGATGGTCA TTATAACGAT 
ACCGATGGTT GCAGCAGCTC CCCCTGTGAA AACGGCGCCT CCTGCTGTGA GGATCCGGAA 
GGAGGCTGGT ACTGTGACTG TCCGCTGGGC TTCCATGGTC CGACATGTGC CTTATTTACC 
AACCCCTGCG TAGACGAAAA CCCCTGCATG GAGGGAGGCA CCTGTGTACC GCCTGACAGT 
TTCTCCCCTG ACTACGCCTA CTGCCTCTGT CCAAGAGGAT TCACAGGTAA AGTGTGTGAG 
ACTACCGTAC CCACCGACTG TACCTTGGGA GAATGTCAGA ACGGAGCAGC CTGTGTAACG 
GACTCTTGGG GAGGTAATGA TGTCTGGTGT GAATGTCTGG AAGGTTTCAC GGGGCCGTAC 
TGTGAGTTTG CGGTTGGAGA ATGTCAAGAA GACAGCTGTG AGCACGGCTC TTGTTATCCG 
GCAGACTGTG GTGGATACTC CTACATGTGC CACTGTGAAG AAGGTACCTG CAGCAACTCC 
CCTTGCCAGA ACAGCGCTGG CTGCTGCGAG GCCCCCGAAG GAGACTGGTA CTGTTACTGC 
CTGCTAGGGT ACGAGGAACC TGGCTGTACA GACTTTAGCG ACCCCTGCAA CTCAGCCTCA 
GGAGAGAACC CCTGCATGCA CGAAGGCACC TGTTATTTCG ACTCCTACAG TAACCAAGCC 
TACTGCCAGT GTCCACCAGG ATTCACAGGG CGAGCGTGCG AGTTTTCTAT GCCCATCGAC 
TGTAACTCAG ACGAGTGTCA GAACGGAGGG ACCTGTATCA TGGAGTCGTG GGGAGATCCC 
TGGTGTAGCT GTCCGGACGG TTTCTCGGGG CCTGTGTGCG AGTTTGCGAT AGGGCAATGC 
GAGGAAGACA CCTGCGTGAA CGGCGACTGC GTAGAATGGA TCTGTGCAGG ATACTACGCC 
TGTAACTGTG ACGAAGGATA TACCGGGACA TTCTGTGAAG TAGAAGATGA TGGTCAGCAC 
AGTGGTGACT ATGGACCAAC CGGGTCTGGA GACTATGGAC CGTACGGCTC TGGAGACTAT 
GGACCGTACG GCTCTGGAGA CTATGGACCT AGCGGTTCTG GGGACTATGG ACCTGGTGAT 
GGTCATTATA ACGATACCGA TGGTTGCAGC AGCTCCCCCT GTGAAAACGG CGCCTCCTGC 
TGTGAGGATC AGGAAGGAGG CTGGTACTGT GACTGTCCGC TGGGGTTCGA TCCTCCGACA 
TGTGCCTACT TTACCAACCC CTGCGTAGAC GAAAACCCCT GCATGGAGGG AGGCACCTGT 
GTACCGCCTG ACAGTTTCTC CCCTGACTAC GCCTACTGCC TCTGTCCAAG AGGATTCACA 
GGTAAAGTGT GTGAGACTAC CGTACCCACC GACTGTACCT TGGGAGAATG TCAGAACGGG 
GGAGCCTGTG AAACGGACTC GTGGGGAGGT GGTGACGTCT GGTGTGACTG CCCAGAAGGT 
TTCACGGGGC CTTACTGTGA GTTTGCGGTT GGAGAATGTC AAGACGACAG CTGTGAGCAC 
GGCTCCTGTT ATCCGGCAGA TTGCGAAGGA TACACTTACA TGTGCCACTG TGAGGAAGGA 
TATTCCGGGA CATTCTGTGA AGTAGAAGGT GATGGTCAGC ACAGTGGTGA CTATGGACCA 
ACTGGGTCCG GAGACTATGG AGCAAGCGGT TCTGGGGACC ATGGACCGTA CGGCTCTGGA 
GACTATGGGC CTAGTGGTTC TGGGGACTAT GGACCTGGAG ACTACGCCGT GTACTACGGC 
TTGGACCCAA ATGGCACTTG TAGCAATTCC CCTTGTCAGA ACAGCGCTGG CTGCTGCGAG 
GCCCCCGAAG GAGACTGGTA CTGCTACTGC CTGCTTGGGT ACGAGGAGCC TGACTGCAGC 
TCCTTTAGTG ATCCCTGCAT GTCAGAAGAG AATCCTTGCA TGAACGAAGG CACCTGTCAT 
TTCGATTCGT ACAGTCGCCA AGCCTACTGC CAGTGTCAAC CAGGATTCAC AGGACGAGCG 
TGTGAGTTTC CAATGCCCAC CGACTGTACC TCAGACGAGT GTCAGAACGG AGGAACCTGT 
ATCATGGAGT CGTGGGGAGA TCCCTGGTGT AGCTGTCCGG ACGGTTTCTC GGGGCCTGTG 
TGCGAGTTTG CGATAGGGCA ATGCGAGGAT GACACCTGCG TGAACGGCCA CTGCGTCGAA 
TGGATCTGTG CGGGATACTA CGCCTGTAAC TGTGACGAAG GATATTCCGG GACATTCTGT 
GAAGTTGAAG GTGATGGCAG TGACTATGGA CCAAGCGGTT CGGGAGACTA TGGACCAACC 
GGGTCTGGGG ACTATGGACC AACCGGGTCC GGAGACTATG TATCACACGG TTCTGGGGAC 
TATGGACCAA ACGGTTCTGG GGACTATGGA CCAAACGGTT CTGGGGACTA TGGACCAAAC 
GGTTCTGGGG ACTATGGACC AAGTGGTTCG GGAGACTATG GACCAACCGG GTCTGGGGAC 
TATGGACCAA CCGGGTCCGG AGACTATGGA TCACACGGTT CTGGGGACTA TGGACCAAAC 
GGTTCTGGGG ACTATGGACC AAACGGTTCT GGGGACTATG GACCAAACGG TTCTGGGGAC 
TATGGACCAA CCGGGTCTGG GGACTATGGA CCAAACGGCT CTGGAGACTA TGGATCACAC 
GGTTCTGGGG ACTATGGACC AAACGGCTCT GGAGACTATG GACCAACCGG GTCTGGGGAC 
TATGGACCAA CCGGGTCTGG GGACTATGGA CCAACCGGGT CCGGGGACTA TGGACCAAAC 
GGTTCTGGGG ACTATGGACC AACCGGGTCC GGTGACTATG GACCATATTA TCCATCTGAG 
GGTCCAGGAC CATATCCATC TGAGGGTCCA GGACCATATC CATCTGCGAG CCCCGAACCT 
TATTATCCAT CTGAGGGTCC AGGACCATAT CCATCTGAGG GTCCAGGACC ACATTATCCA 
TCTGAGGGTC CAGGACCATA TCCATCTGAG GGTCCAGGAC CATATCCATC TGCGAGCCCC 
GAACCTTATT ATCCATCTGA GGGTCCAGGA CCATATCCAT CTGAGGGTCC AGGACCACAT 
TATCCATCTG AGGGTCCAGG ACCATATCCA TCTGAGGGTC CAGGACCATA TCCATCTGAG 
AGCCCCGGAC CATATTATCC ATCTGAGGGT CCAGGGCCAT TTCCATCTGA GGGCCCTGGA 
CCATATTATC CATCTGAGGG TCCAGGGCCA TTTCCGTCTG AGGACCCTCG ACCAATTAAC 
CATACCGAGG GTTGCAGCAG CTCCCCCTGT GAAAACGGCG CCTCCTGCTG TGAGGATCCG 
GAAGGAGGCT GGTACTGTGA CTGTCCGCTG GGGTTCGATC CTCCGACATG TGCCACCTTT 
ACCAACCCCT GCGCTGGGGA AAACCCCTGC ATGAAGGGAG GCACTTGTGT ACCGCCAGAC 
ATTTTCTCCC CTGACTACGC CTACTGCCTC TGTCCAAGAG GATTCACAGG TAAAGTGTGT 
GAGACCACCG TACCAACCGA CTGTGCTTTA AACGAATGTC AGAACGGGGC AGCATGTCAC 
ACGGACTCCT GGGGAGGTGG TGACGTCTGG TGTGACTGCC CAGAAGGTTT CACGGGGCCT 
TACTGTGAGT TTGCGGTTGG AGAATGCCAA ATTGACAGCT GTGAGCACGG CTCCTGTTAC 
CCGGCAGACT GTGATGGATA CTCCTACATG TGCGACTGTG AGGAAGGATA CTCCGGGACA 
TTCTGTGACG TAGAAGGTGA TGGTGGTGAC TATGGACCAA CTGGGTCTGG AGAATATGGA 
CCGAGTGGTT CTGGGGACTA TGGGCCATAT TATCCATCTG AGGGCCCTGG ACCATATCCA 
TCTGAGGGTC CAGGACCACA TCCCTCTGAG GACCCTCGAC CATATAACCC CACTGACGAC 
CCTGGATCAT ATACTACCGA GGTCCCCGGG CCAAATCCGC TTGAAGACTC CAACCTGTAC 
CCGTTCGGTC CGGACGCTGG TGATTTGGAG TATGAGGACC TCCTGAACAG TGGCGACGGC 
CATTATTGGG GAGAGTGTTT GGAGCAGAGT ATCAACGATG AGGGCTTCCC GTTCTATGGA 
AAGAGGCACT ACAAACTCTA TGTTTGTGAC AATGGCATGA TCTCCTTCGA GACGCCATGG 
GCGCCATGGG TGCCGTATCT GTTCGGCTCC CGTCGCATGT ACAGCGACAT CGCCATGATC 
GCGCCGTTCT GGGCCATGTC AGACGCGTCC AGCCTCTCCC AGGACAGGCA GGACACCGAG 
CATTCGCACG TCTACTTCCA CTCGTACGCC GAAGGAGACG GGCAGGCAAA CACCGAGGAG 
ATCCTGAATC GCGCAGGAGA AGATGCGAAC GACTACGATG CATCCATACC AGATTTCAGG 
CCAACATGGG CCCTAGTGGT GACATGGGTC CGCCTTCCCC CGTACATCGG CTGCAGCTGT 
TACCGGGGCT GGGGCTGCCA GGAGTGTGAC CTCACTGACG TACAGACGAA CACGTTCCAG 
GCCGTGCTGA TGACAAACGC CCTGCATTCC TTCGGCAAGT CTATCTACCC GCAGGGAGGG 
CTCAACTGGG TTTATCCAGG ATTTAGCGAT GAGTACTACC AGTACTTCAC GGATGTCCAG 
GACCTACCAT GGCCCGTGGC AGGCTTCAGT GCCGGGGACG TGGGACAGAA CGTCATCAAC 
CTCCCAACGT ACTCAGGAAC CCAGCGGATG CACCTACTGG ATGAGGTCGA GGGGAACACA 
GGCTACGTTG GTCAGTGGAT GGTGCGGATG GAGGACTCGG ACGGCACCAT GGACGCGCGG 
CTGGAGTGTC TGGCCTGGCA CAGGGACCAG CCGGACCCCG CGGACGTGCA GGCCAGTCTG 
AGTGAGCTGC CGCCGGACTG CCCCTGCACG GAGTGGCAGC TGTGGTTTGA TGGAGCGTTC 
CAGATCGACT GGAGCAGCTG GGGCTGGGAT ATCTGCGCCT ACAGCGCCTT CCCCACAGCG 
GACGGGCTGG TGCAGAAATG CTGCTATGAA GACGGCGCGC TGAAGACCGG AGGTCCGGAT 
GGGGGATACG TGACTCAGAA CCGTGCAGAT GACGAGTTGG CATACGCAAA ATGCTGCGTG 
GAAAGTGTGG GGACCTGTGA TCTGTTCTAC GAGAAGCGTC CATCGGCTAA CTGCTCTCAG 
TACCTCCCAC CCATATGGAG TAGGTGTATT TTATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_105734 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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