Pla2 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pla2
Entrez Gene ID	406141
Full Name	phospholipase A2
Synonyms	GB13351,bvPLA2
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011614.1	NP_001011614.1	phospholipase A2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Surface pressure-dependent interactions of secretory phospholipase A2 with zwitterionic phospholipid membranes.
Huang WN, Chen YH, Chen CL, Wu W
Langmuir : the ACS journal of surfaces and colloids27(11)7034-41(2011 Jun)

Disulfide bonds of phospholipase A2 from bee venom yield discrete contributions to its conformational stability.
Welker S, Markert Y, Köditz J, Mansfeld J, Ulbrich-Hofmann R
Biochimie93(2)195-201(2011 Feb)

Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus.
Valdez-Cruz NA, Segovia L, Corona M, Possani LD
Gene396(1)149-58(2007 Jul)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pla2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pla2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00136	NM_001011614.1 Latest version!	Apis mellifera phospholipase A2 (Pla2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pla2 ( NM_001011614.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011614.1 Apis mellifera phospholipase A2 (Pla2), mRNA.

Price & Availability↑

CloneID	OHd00136
Clone ID Related Accession (Same CDS sequence)	NM_001011614.1
Accession Version	NM_001011614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569081600000
Organism	Apis mellifera(honey bee)
Product	phospholipase A2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF438408.1. On Feb 5, 2005 this sequence version replaced XM_391951.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF438408.1, HP469947.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00996631, SAMN02719778 [ECO:0000348] ##Evidence-Data-END##

ATGCAAGTCG TTCTCGGATC CTTGTTCCTT CTCCTCCTCT CTACCTCTCA CGGATGGCAA 
ATCAGGGATA GGATCGGGGA TAACGAGTTG GAGGAACGGA TAATATATCC AGGAACGTTA 
TGGTGCGGGC ATGGTAACAA GTCGTCCGGC CCGAACGAGC TAGGTCGGTT CAAGCACACG 
GATGCATGCT GTCGAACCCA CGACATGTGC CCGGACGTGA TGTCAGCTGG TGAATCGAAG 
CACGGCCTGA CCAACACGGC CTCCCACACC AGGTTGTCGT GCGACTGCGA CGACAAGTTC 
TATGATTGTC TTAAAAATTC GGCGGACACG ATTAGCTCGT ATTTCGTAGG GAAGATGTAC 
TTCAATCTGA TAGACACGAA GTGTTACAAA CTGGAGCATC CTGTCACCGG GTGCGGTGAG 
AGAACCGAGG GTCGTTGTCT TCACTACACC GTGGACAAAA GCAAACCGAA AGTGTACCAA 
TGGTTCGATC TTCGCAAGTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011614.1
CDS	135..638
Translation	MQVVLGSLFLLLLSTSHGWQIRDRIGDNELEERIIYPGTLWCGHGNKSSGPNELGRFKHTDACCRTHDMCPDVMSAGESKHGLTNTASHTRLSCDCDDKFYDCLKNSADTISSYFVGKMYFNLIDTKCYKLEHPVTGCGERTEGRCLHYTVDKSKPKVYQWFDLRKY

Target ORF information:

RefSeq Version	NM_001011614.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera phospholipase A2 (Pla2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011614.1

ORF Insert Sequence:

ATGCAAGTCG TTCTCGGATC CTTGTTCCTT CTCCTCCTCT CTACCTCTCA CGGATGGCAA 
ATCAGGGATA GGATCGGGGA TAACGAGTTG GAGGAACGGA TAATATATCC AGGAACGTTA 
TGGTGCGGGC ATGGTAACAA GTCGTCCGGC CCGAACGAGC TAGGTCGGTT CAAGCACACG 
GATGCATGCT GTCGAACCCA CGACATGTGC CCGGACGTGA TGTCAGCTGG TGAATCGAAG 
CACGGCCTGA CCAACACGGC CTCCCACACC AGGTTGTCGT GCGACTGCGA CGACAAGTTC 
TATGATTGTC TTAAAAATTC GGCGGACACG ATTAGCTCGT ATTTCGTAGG GAAGATGTAC 
TTCAATCTGA TAGACACGAA GTGTTACAAA CTGGAGCATC CTGTCACCGG GTGCGGTGAG 
AGAACCGAGG GTCGTTGTCT TCACTACACC GTGGACAAAA GCAAACCGAA AGTGTACCAA 
TGGTTCGATC TTCGCAAGTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Surface pressure-dependent interactions of secretory phospholipase A2 with zwitterionic phospholipid membranes.
Langmuir : the ACS journal of surfaces and colloids27(11)7034-41(2011 Jun)
Huang WN,Chen YH,Chen CL,Wu W

Disulfide bonds of phospholipase A2 from bee venom yield discrete contributions to its conformational stability.
Biochimie93(2)195-201(2011 Feb)
Welker S,Markert Y,Köditz J,Mansfeld J,Ulbrich-Hofmann R

Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus.
Gene396(1)149-58(2007 Jul)
Valdez-Cruz NA,Segovia L,Corona M,Possani LD

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)