ORF » Species Summary » Leishmania major strain Friedlin » LMJF_15_0050 cDNA ORF clone

LMJF_15_0050 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_15_0050 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_15_0050 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb01510 XM_001681867.1
Latest version!		 Leishmania major strain Friedlin putative cytochrome-b5 reductase partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_15_0050 ( XM_001681867.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001681919.1 Leishmania major strain Friedlin putative cytochrome-b5 reductase partial mRNA.
    Price & Availability↑
    CloneID OLb01510
    Clone ID Related Accession (Same CDS sequence) XM_001681867.1
    Accession Version XM_001681867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative cytochrome-b5 reductase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007256). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTGGAGTA CCAGGCAGCA GTCGGAGCGC TTAGCGGCCA CCTGCGCCAA GAAACCAGTG 
    GATACGTTCT CCCCCGATGC GTACAAGCCA TTTAAGCTGA TTTCCTCTCG GTACGAGTCG 
    CACGATACGC GCCGCTTTTA CTTTGCCCTC GATAGCCCCG ATGATAGCTT CTACATGCCA 
    GTTGCTTCGT GCATCATCGC GAAGTACACC GATGCAGACG GCAAGGACGT TGCTCGGCCA 
    TACACTCCCA TCTCCTCTAA TAGCACCAAG GGCCACTTTG AACTTGTTGT GAAGAAGTAT 
    CCAAAAGGAA AAATGGGCAA CTATCTGTTC TCCATGCAAC CAGGCGACGA ACTGCTCGTC 
    AAGGGCCCTT TTGAGAAGTT TGCGTACAAG CCCAACATGT GGAAGCACGT GGGCATGATC 
    GCCGGTGGTA CCGGCATCGC TCCAATGTAC CAGGTGCTTC GTGCCGTGCT AGAGAATCCC 
    AGAGACAAGA CCAACATTTC TCTCATCTAT GCCAACAACC AGCGCCGCGA CATTCTGCTT 
    GCTAACGAAC TGATTGAGAT GCAGAAGGTC TACACCAACT TCAACATGTA TTTGACCCTT 
    CTGGAGGTGC CGCACCGATG GCTAGGCGGT ATCGGCTATG TGAATAGTGC CATGGTAACC 
    ACCTTTATGC CGAAGCCGGG GGAGAAGAAC ACGAAGATCC TCGTATGTGG CCCCCCTCCG 
    ATGATGCAGG CCATCAGCGG CGACAAGCTG TTTGAGCCTG GCAAGCCGCC CCAGCAGGGC 
    CAGGTAGGCG GCCTGCTGAA AACGCTAGGC TACACGGAGG AGCAGGTCTT CAAGTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001681919.1
    CDS1..840
    Translation

    Target ORF information:

    RefSeq Version XM_001681867.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative cytochrome-b5 reductase partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001681867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTGGAGTA CCAGGCAGCA GTCGGAGCGC TTAGCGGCCA CCTGCGCCAA GAAACCAGTG 
    GATACGTTCT CCCCCGATGC GTACAAGCCA TTTAAGCTGA TTTCCTCTCG GTACGAGTCG 
    CACGATACGC GCCGCTTTTA CTTTGCCCTC GATAGCCCCG ATGATAGCTT CTACATGCCA 
    GTTGCTTCGT GCATCATCGC GAAGTACACC GATGCAGACG GCAAGGACGT TGCTCGGCCA 
    TACACTCCCA TCTCCTCTAA TAGCACCAAG GGCCACTTTG AACTTGTTGT GAAGAAGTAT 
    CCAAAAGGAA AAATGGGCAA CTATCTGTTC TCCATGCAAC CAGGCGACGA ACTGCTCGTC 
    AAGGGCCCTT TTGAGAAGTT TGCGTACAAG CCCAACATGT GGAAGCACGT GGGCATGATC 
    GCCGGTGGTA CCGGCATCGC TCCAATGTAC CAGGTGCTTC GTGCCGTGCT AGAGAATCCC 
    AGAGACAAGA CCAACATTTC TCTCATCTAT GCCAACAACC AGCGCCGCGA CATTCTGCTT 
    GCTAACGAAC TGATTGAGAT GCAGAAGGTC TACACCAACT TCAACATGTA TTTGACCCTT 
    CTGGAGGTGC CGCACCGATG GCTAGGCGGT ATCGGCTATG TGAATAGTGC CATGGTAACC 
    ACCTTTATGC CGAAGCCGGG GGAGAAGAAC ACGAAGATCC TCGTATGTGG CCCCCCTCCG 
    ATGATGCAGG CCATCAGCGG CGACAAGCTG TTTGAGCCTG GCAAGCCGCC CCAGCAGGGC 
    CAGGTAGGCG GCCTGCTGAA AACGCTAGGC TACACGGAGG AGCAGGTCTT CAAGTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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