CEP250 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	CEP250
Entrez Gene ID	100032480
Full Name	centrosomal protein 250
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007474241.2	XP_007474303.1	centrosome-associated protein CEP250 isoform X1
XM_007474242.2	XP_007474304.1	centrosome-associated protein CEP250 isoform X2
XM_007474240.2	XP_007474302.1	centrosome-associated protein CEP250 isoform X1
XM_007474243.2	XP_007474305.1	centrosome-associated protein CEP250 isoform X3
XM_016427979.1	XP_016283465.1	centrosome-associated protein CEP250 isoform X1

Pathways from BioSystems
Gene Ontology

The following CEP250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CEP250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc04275	XM_007474240.2 Latest version!	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMc04275	XM_016427979.1 Latest version!	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMc04275	XM_007474241.2 Latest version!	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMc04276	XM_007474242.2 Latest version!	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMc04277	XM_007474243.2 Latest version!	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CEP250 ( XM_007474241.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007474303.1 Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMc04275
Clone ID Related Accession (Same CDS sequence)	XM_007474241.2 , XM_007474240.2 , XM_016427979.1
Accession Version	XM_007474241.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007474241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
AAGATGGATC AAATCAAGCA CAGGCAAGAA TTAGAGGCCA CATTAGAACA AATGAAAAGA 
GAGAAAGTGG ACCTGGAGGA GACCTGTGAG AAGAAACTAA GGGAACTGCA GGAGGAGTCA 
GAAGCTGCCC TGGCTCAGAG GATAAAGGAT CATGCCCAAG CTGAGAGCAA CAGATGTCAG 
ATGCAGCTAG AGATGGAAAA GGAAAGGATG TCACTACTGG AGACACTCTT GAAGACCCAG 
AATGAACTAG CTGATGCTGG CCATCAATTG GAGCAGCTGA GGCAGGACAT AAAGGACCAG 
AAGTTAAAGG AACAGGAGAA TATGGAGGAC CTTTGGGCCC AGCTGCAAGA GGCTCAGAAG 
ACACTGATGG AGACAAGAAG AAAGCATCAC AATGACCTTG CTGCCCTTGA GGAAGAGAAA 
AGCATCCTGC TACAGCAAAA AGAGAACCTG GAACAACAGG TACAAGACTT GAAGGCCCAA 
CTAGTCACAA GTGAAGATTC ATGTAGGCTG GCAGAGCATG AGGCCCAGAA GCATCTACAA 
GAGGTCCAGC AGTGTACTCA TATCCAGGAG GAGAGGGAGC AGGAAAAGGC CAGCCTGGTT 
CAGTCACTAG CAGAGAAGGA ACAAAGACTC CTCATTTTAC AAGAGGCTGA TTCACTCCAA 
CAGCAAGAGC TGAGTTCCTT GCGTCAGGAA GTGAAGGAGG CCCAGGAAGA ACAGGAGCAG 
CTCAGTGCCC AGGTACTGTT TCTAAAGGAA GAGATGCAGA AAAAAGAAGC TTCTCTTTTG 
GATCAAGAAA TCCTTTTGGA GAAGTTGGGA GCATCAAAGA AAACAGAGCA GCAGCTGAGA 
GCCTCACTGT GGGCACAAGA GGCCAAGGCA GCCCAGCTGC AACTCCAGCT GTGCAGTGTA 
GAAAGCCAAG TAGGGGCTCT AATATTAGAG CAGCAGAACG GACATCAGGC ACAGGCACAG 
CTAATGGGTC TCTACTCCAT TCTGCAGCAA GCCTTTGGAA GTGCTATTGA GAGTCGGCCA 
GAGCTGAGTG GTGCGGGTGA TGCTTCTCCC CTTTCCTGGA GCCCCAGAAC TTTGGAGCCA 
GGACAAGAGA ATTTCCTTAA AAACCAGATT GCTGTAACGA ACTTTACTAC TGAGGATGTG 
GCATCTTTAC TTCACAAGCT CCAGCGAGAC CTCTGGCAGG CTCGTCATAC TCGGGATGAT 
ATGAAATGTC AGGTTGAGAA GCTGGAGCAA CATCTGTCCC AAGCTGAGGC TGAGCGGAAC 
CAGTTTTCTA CGGAAATTCA AGAGCTTCAA AGTTTGCTCT CCCAGAATCA AAAAGAGAAA 
TTGAAATGGG AGGGAGAGCA GAGCTCTTTG GAGACAGAGT TGGCTGTACT TCAGGAAAGA 
GTGGCTTCCC TGCAGAACAG TCTGAAGAGA TCTGAGCAGC AAAGGACAGA AGCCCAGAAT 
GAGCAGGCAA CATTGTGGGC AGCCAATGAA AAATTGGTAT CAGAGGTGGA ACACCTTCAA 
GCATCAGTCG CAGTCACCAA GGCACAAGCT AATGCCAGGG AGGCCCTGGA AGCAGAACTG 
AGAGCAACAC ACTCAGCCCT GAGAATGAAA AATGAAGAAG CAGAGAGTGA GCGAGAGAAA 
GCAGAGGCCC TAAGGAAGAA GCTGGCCCAA GGGAAGGCCC TGCAGGAAAA TGTTGATCTC 
TTGACCAGTA CCCTTTCTAA GAGAGAGAAG GAATTAGAAG CCCTGCAGGG AGTCATTCAA 
GAACTGGAGA AACAGAGAGA ATCACAAATG GTGATGCAAG AACATCTGTC TCTGAACCTA 
AAGAAGAGAA GTCAAGAGCT GGAATCCCGG CAAGAACAGA TCCAAGAGCT GGAGAAACAG 
AAAGCTGGAC TAGAACATCT GCCTGTGGCC CTGGAACAGA AAAAGAAACA AATACGGTTA 
CAGAGAGAGC AGATTAAAGA GCTTGAGGGA AAGCAAGAAA CTCAGGGAAA CATCCTGGAA 
CATCAAGCTC TGGATTTACA GAAGAAAACA CAAACATTAG AGTCCCAGAG AGAACATATT 
CAAGATCTGG AAAACCAGTT GGTCACTCTG GAACATCTGG TTTTAGAAGT GAAAAAGAAT 
CATCAAGAAA TAGAGTCTCA GAAAAAAAAG ATTGAGGACT TAGAGAACCA GAGAGAAATG 
CAGAGGGTCA CTCTGACCCA TCTTACTTTG GACTTGGAGG AGAAAAGTCA GGAAATACAA 
TCTCAGAACA AAAAGATTAA AGAGTTAGAA AAGCACAATG CTCTCCTGAC AGTAAATCTG 
GAGGAGAGAT GCCAAGAGAT GAAGTCCTAC AGAGACCAGG TGGAAGAGTT AAAGAAGCAA 
AAGGATCAGC TTACTTGGGA CCTAGAGAAA AAAAACCAGG AGTTGGTCCT ACAAGAAGAG 
AAAATTCTGG TCCTGGAAGA TCAGAGAATG TTGCAGAACA GAGTATTAGA GGAAGACTTT 
GAACAGATGA AATTGGCCTT GAGGGAAAAA GATCAGGAAA TTGAGTCTCA AAGACAGCTC 
ATGATAGAAC GGGAAGAGGA AGAAAAGGGA CAAGCCAAGG CCCAACGCAG CAGCCTGGAG 
CATATGAAGT TGATCCTGAG GGACAAGGAG AAGGAGGTCG AGTACCAGCG AGAGAGGATT 
CACATGTTCC AACAGTATGG AGAACAGCGG GAAGAGCAAC TACAGGGCCT GCGGAGAAAG 
ATTGGGGAGA TGACGCTGCT CTTAACTCAG AAAGATCAAG AGTTGGAGAC CCAACAACAG 
CATATCAGGA GAGTCCAGGA GAGACGGGAG TCCAGGGAAA GGGCCCTGCA GGACCAACTG 
GAGAGGGTGC AGGAGGCCTT GCAGACCAAA GTGCAAGACC TGGAAGCCCT CCAGCAGAAG 
GCACAATTAC AGCAGGAAAG CCAGGAAGAA CAGATGAGCA CTTTGCAAGC AGCCCTGGAA 
CAGGCCAGGG CAGCTCTGGA GGAGCATGAG GAGGCAATTG AGGCTCACAA GGAACAGAAC 
CAGAAAGTAC AGCAACAACA AACGGCTGCA GAGCTGCAGG TTCAAGCTTT GGAAGAGCTG 
CTAGGAGATC TCAGGGCTGA GTTTCAGGAG CAAGAGAAGG TTCTGGAGAC CCTGCGACAG 
CAGTGTACAG AGCAAGGACA TGAGCAGGAG ACACAGGCCT GGCACCAGCA CACAGAAGCC 
AGGCAGTGGG AAAAAACTAC AGCAGGCCAG GTCCAGGCCC TCAAGGAAGC CCTCAGAGTG 
GCCCAGGCCT CCCTTCAGAA GAAAGACCAG GAGCTCCTCC AGCAGGCAGA ACTAAGCCAG 
CGCTTGGAAG GCAGTGCAGC CGCTTTGCAG GTGGCCTTGG ACTCATGTCA GACTCAGGCC 
CGGCACCTGG AGGAGGCCCT GAGGGAGCGA GAGGAAGAGA TCAGGGAGCA GCAGCTACAG 
CACCAAGAAA TGGTACAGCA ACTGCAGCTG GCCCTGGCCC AGAGGGATGG GGAGCTGAGG 
CAACTCAAGG AAAAGGGGCA GCAGCAAGAG GCAGCCCTGA ACCAATCAGA CAAAGGTGAG 
GAAAAAGAAG AGAAGGTCCT GAGACAAAAC CTCAGGCATC TCCAGCTGAC CTTGTCCAGA 
AAGGAGTTCC AGCTGCAGGA GACCCACCGG AGGCATGGAC AGGAGTCTTA CTATGAAAGC 
CACAGCCCAG AGAAAATGGA GGATGGGGAG GAGGAGGAGG TCTATGGGAC AGGGACGATG 
GAGAAAATGT CCCTCCGTGA GATATCGCCT GTGTCCATGG AAGAGGCCAA GCTGCAAGAG 
GAGCTTGAGA GAGTGAAGAA TGTCCTGAGG CAAACAGAGG CTCGGGAAAT TGAGTGGCAA 
GAGAAGGCCC AAGCCTTGGC ATTCTCCCTA GCTGAGAGTG AAGCCAGCAT CTGTAACCTG 
CGTGAGGCTG CCCTATTCTT ACAGGCAAAG ACAGCAGAGC AAAACACAGA ACAGCTACAA 
CTTGAGGAGC AGTTGCAGCT CACAAGACAA GCTCTAGAGG ATGAGCAATC CCACAACCGG 
GGCTCAGCTT GGAGGACAGA GCCAGAGATC CAGGGAGATG AGGGTACTTC CCAAAGAAAG 
GCCACAGGAC CAGAGGATGA TGGATCTTGT ACAGAACTTG AGAAGACACA GCAATGGCAG 
CAAAGGCTAA AGTATCTGCT ACATGCTATG GCAAGTCTAG AGCATGAGAG GAACCGGCTT 
CAGCGACACA ACTTCCAATT GAGGGTCACC TTAGAGCAGG TAGAACGGGA GCGGAGGAAA 
CTGAAGAGGA ATTTTAACCG TATGACCCAT ACTACACCTC CCCTGCCTAG GGAGACCACA 
GCCCTCACAC CAGGCCAGAA GCAGGAGAAA GGGGGACAGG AGAATTCCTC AGAATTGAAA 
CATGTGGCTG AGTTACAGAG GCAGGTGACA GCCCTGCAGA CACAGCTGGC ACTGGAGCGG 
CAGCAGAAAC AGGACTACAT CTCCCGCTGT GTGCAGACAA GCCGAGAGCT AACAGATCTG 
CACCACAACC TTTCCAACTC ACTGTGGGCT GTAGCTCGGG CCCCTGAAGC CAACATCCTG 
GAGGCAGAGG CCCGAAAGCT AGATAAGTCA CTGACCCAGA GCCTGACATC TTCCGCACCA 
TCCCCACCCT GCTGCACTCC CAGGTCAGCC CAGGCTACCT CCCGTAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007474303.1
CDS	276..7526
Translation	MEARDQGHNMKPQPLNMVLEKQILALQQQMAENQAASWHKLKISQEAQQRQAVLVRKLQAKVLQYRNWCQELEKRLEDMGGPMPKRWTSAEEPRLEQLLIRLEEEHQRSEGLVKVNTQLQEHLEEADVVNKALQEDVGKLTVDWSQAQDELARRQSDWQMEREFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKTATDRDLAELKAEQVKISGSLLTSCLHLSLELQSREAQGSGRLAGNEQVHLQLVAKTQELEKEIHKRSQELILLKARGNMEKTELQDRVTELSALLAQSKKQNEEQEKMLKALKDTMDILETNRSELMEHEASLSRNSQKEKLSLQQVIKDITQVVVDDEDSENQSFGLESSLEMESSIFCQLRSEDPDKAVTLVRSILAKKHQAVQGLRQQLSTCQEAVSFWQHQHTQWEEEGEALRQRLQKLTGERDVLAGQTQGLQGEVDSLNRDRGHLEKAREELQQQLEILEQEAWRLRRLNTELQLQGDLAQGEKVEQEEELQQAVRERDCLQETLKGLEARQSSSLSELISLKEALEKSHLEGELLNQERAEMARALAKAEQSIAELTGTERTLKAEVADLRDAAAKLSALNEALALDKVELNQQLLQLEQENQSVHGKVEASEKARSAVQMELDESEQRKEALLEEKACLEMQLQKSKDAEAELQGDLSREREEKIETQEKLNEAQRQLEVATTELEQLHQETTRQELLREKVIQEKEILVRDKAALEVHLKALERDQQDLSEQLLELSSAKELLETNLFEAQQQGSLMEITKEQLMMQIQTVTQAKEVIQGEVKCLQMELEAQRQQAEQEQDTAAKHRDQMEQESQAALQQQRATYEEELHLLQEERKMDQIKHRQELEATLEQMKREKVDLEETCEKKLRELQEESEAALAQRIKDHAQAESNRCQMQLEMEKERMSLLETLLKTQNELADAGHQLEQLRQDIKDQKLKEQENMEDLWAQLQEAQKTLMETRRKHHNDLAALEEEKSILLQQKENLEQQVQDLKAQLVTSEDSCRLAEHEAQKHLQEVQQCTHIQEEREQEKASLVQSLAEKEQRLLILQEADSLQQQELSSLRQEVKEAQEEQEQLSAQVLFLKEEMQKKEASLLDQEILLEKLGASKKTEQQLRASLWAQEAKAAQLQLQLCSVESQVGALILEQQNGHQAQAQLMGLYSILQQAFGSAIESRPELSGAGDASPLSWSPRTLEPGQENFLKNQIAVTNFTTEDVASLLHKLQRDLWQARHTRDDMKCQVEKLEQHLSQAEAERNQFSTEIQELQSLLSQNQKEKLKWEGEQSSLETELAVLQERVASLQNSLKRSEQQRTEAQNEQATLWAANEKLVSEVEHLQASVAVTKAQANAREALEAELRATHSALRMKNEEAESEREKAEALRKKLAQGKALQENVDLLTSTLSKREKELEALQGVIQELEKQRESQMVMQEHLSLNLKKRSQELESRQEQIQELEKQKAGLEHLPVALEQKKKQIRLQREQIKELEGKQETQGNILEHQALDLQKKTQTLESQREHIQDLENQLVTLEHLVLEVKKNHQEIESQKKKIEDLENQREMQRVTLTHLTLDLEEKSQEIQSQNKKIKELEKHNALLTVNLEERCQEMKSYRDQVEELKKQKDQLTWDLEKKNQELVLQEEKILVLEDQRMLQNRVLEEDFEQMKLALREKDQEIESQRQLMIEREEEEKGQAKAQRSSLEHMKLILRDKEKEVEYQRERIHMFQQYGEQREEQLQGLRRKIGEMTLLLTQKDQELETQQQHIRRVQERRESRERALQDQLERVQEALQTKVQDLEALQQKAQLQQESQEEQMSTLQAALEQARAALEEHEEAIEAHKEQNQKVQQQQTAAELQVQALEELLGDLRAEFQEQEKVLETLRQQCTEQGHEQETQAWHQHTEARQWEKTTAGQVQALKEALRVAQASLQKKDQELLQQAELSQRLEGSAAALQVALDSCQTQARHLEEALREREEEIREQQLQHQEMVQQLQLALAQRDGELRQLKEKGQQQEAALNQSDKGEEKEEKVLRQNLRHLQLTLSRKEFQLQETHRRHGQESYYESHSPEKMEDGEEEEVYGTGTMEKMSLREISPVSMEEAKLQEELERVKNVLRQTEAREIEWQEKAQALAFSLAESEASICNLREAALFLQAKTAEQNTEQLQLEEQLQLTRQALEDEQSHNRGSAWRTEPEIQGDEGTSQRKATGPEDDGSCTELEKTQQWQQRLKYLLHAMASLEHERNRLQRHNFQLRVTLEQVERERRKLKRNFNRMTHTTPPLPRETTALTPGQKQEKGGQENSSELKHVAELQRQVTALQTQLALERQQKQDYISRCVQTSRELTDLHHNLSNSLWAVARAPEANILEAEARKLDKSLTQSLTSSAPSPPCCTPRSAQATSRS

Target ORF information:

RefSeq Version	XM_007474241.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007474241.2

ORF Insert Sequence:

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
AAGATGGATC AAATCAAGCA CAGGCAAGAA TTAGAGGCCA CATTAGAACA AATGAAAAGA 
GAGAAAGTGG ACCTGGAGGA GACCTGTGAG AAGAAACTAA GGGAACTGCA GGAGGAGTCA 
GAAGCTGCCC TGGCTCAGAG GATAAAGGAT CATGCCCAAG CTGAGAGCAA CAGATGTCAG 
ATGCAGCTAG AGATGGAAAA GGAAAGGATG TCACTACTGG AGACACTCTT GAAGACCCAG 
AATGAACTAG CTGATGCTGG CCATCAATTG GAGCAGCTGA GGCAGGACAT AAAGGACCAG 
AAGTTAAAGG AACAGGAGAA TATGGAGGAC CTTTGGGCCC AGCTGCAAGA GGCTCAGAAG 
ACACTGATGG AGACAAGAAG AAAGCATCAC AATGACCTTG CTGCCCTTGA GGAAGAGAAA 
AGCATCCTGC TACAGCAAAA AGAGAACCTG GAACAACAGG TACAAGACTT GAAGGCCCAA 
CTAGTCACAA GTGAAGATTC ATGTAGGCTG GCAGAGCATG AGGCCCAGAA GCATCTACAA 
GAGGTCCAGC AGTGTACTCA TATCCAGGAG GAGAGGGAGC AGGAAAAGGC CAGCCTGGTT 
CAGTCACTAG CAGAGAAGGA ACAAAGACTC CTCATTTTAC AAGAGGCTGA TTCACTCCAA 
CAGCAAGAGC TGAGTTCCTT GCGTCAGGAA GTGAAGGAGG CCCAGGAAGA ACAGGAGCAG 
CTCAGTGCCC AGGTACTGTT TCTAAAGGAA GAGATGCAGA AAAAAGAAGC TTCTCTTTTG 
GATCAAGAAA TCCTTTTGGA GAAGTTGGGA GCATCAAAGA AAACAGAGCA GCAGCTGAGA 
GCCTCACTGT GGGCACAAGA GGCCAAGGCA GCCCAGCTGC AACTCCAGCT GTGCAGTGTA 
GAAAGCCAAG TAGGGGCTCT AATATTAGAG CAGCAGAACG GACATCAGGC ACAGGCACAG 
CTAATGGGTC TCTACTCCAT TCTGCAGCAA GCCTTTGGAA GTGCTATTGA GAGTCGGCCA 
GAGCTGAGTG GTGCGGGTGA TGCTTCTCCC CTTTCCTGGA GCCCCAGAAC TTTGGAGCCA 
GGACAAGAGA ATTTCCTTAA AAACCAGATT GCTGTAACGA ACTTTACTAC TGAGGATGTG 
GCATCTTTAC TTCACAAGCT CCAGCGAGAC CTCTGGCAGG CTCGTCATAC TCGGGATGAT 
ATGAAATGTC AGGTTGAGAA GCTGGAGCAA CATCTGTCCC AAGCTGAGGC TGAGCGGAAC 
CAGTTTTCTA CGGAAATTCA AGAGCTTCAA AGTTTGCTCT CCCAGAATCA AAAAGAGAAA 
TTGAAATGGG AGGGAGAGCA GAGCTCTTTG GAGACAGAGT TGGCTGTACT TCAGGAAAGA 
GTGGCTTCCC TGCAGAACAG TCTGAAGAGA TCTGAGCAGC AAAGGACAGA AGCCCAGAAT 
GAGCAGGCAA CATTGTGGGC AGCCAATGAA AAATTGGTAT CAGAGGTGGA ACACCTTCAA 
GCATCAGTCG CAGTCACCAA GGCACAAGCT AATGCCAGGG AGGCCCTGGA AGCAGAACTG 
AGAGCAACAC ACTCAGCCCT GAGAATGAAA AATGAAGAAG CAGAGAGTGA GCGAGAGAAA 
GCAGAGGCCC TAAGGAAGAA GCTGGCCCAA GGGAAGGCCC TGCAGGAAAA TGTTGATCTC 
TTGACCAGTA CCCTTTCTAA GAGAGAGAAG GAATTAGAAG CCCTGCAGGG AGTCATTCAA 
GAACTGGAGA AACAGAGAGA ATCACAAATG GTGATGCAAG AACATCTGTC TCTGAACCTA 
AAGAAGAGAA GTCAAGAGCT GGAATCCCGG CAAGAACAGA TCCAAGAGCT GGAGAAACAG 
AAAGCTGGAC TAGAACATCT GCCTGTGGCC CTGGAACAGA AAAAGAAACA AATACGGTTA 
CAGAGAGAGC AGATTAAAGA GCTTGAGGGA AAGCAAGAAA CTCAGGGAAA CATCCTGGAA 
CATCAAGCTC TGGATTTACA GAAGAAAACA CAAACATTAG AGTCCCAGAG AGAACATATT 
CAAGATCTGG AAAACCAGTT GGTCACTCTG GAACATCTGG TTTTAGAAGT GAAAAAGAAT 
CATCAAGAAA TAGAGTCTCA GAAAAAAAAG ATTGAGGACT TAGAGAACCA GAGAGAAATG 
CAGAGGGTCA CTCTGACCCA TCTTACTTTG GACTTGGAGG AGAAAAGTCA GGAAATACAA 
TCTCAGAACA AAAAGATTAA AGAGTTAGAA AAGCACAATG CTCTCCTGAC AGTAAATCTG 
GAGGAGAGAT GCCAAGAGAT GAAGTCCTAC AGAGACCAGG TGGAAGAGTT AAAGAAGCAA 
AAGGATCAGC TTACTTGGGA CCTAGAGAAA AAAAACCAGG AGTTGGTCCT ACAAGAAGAG 
AAAATTCTGG TCCTGGAAGA TCAGAGAATG TTGCAGAACA GAGTATTAGA GGAAGACTTT 
GAACAGATGA AATTGGCCTT GAGGGAAAAA GATCAGGAAA TTGAGTCTCA AAGACAGCTC 
ATGATAGAAC GGGAAGAGGA AGAAAAGGGA CAAGCCAAGG CCCAACGCAG CAGCCTGGAG 
CATATGAAGT TGATCCTGAG GGACAAGGAG AAGGAGGTCG AGTACCAGCG AGAGAGGATT 
CACATGTTCC AACAGTATGG AGAACAGCGG GAAGAGCAAC TACAGGGCCT GCGGAGAAAG 
ATTGGGGAGA TGACGCTGCT CTTAACTCAG AAAGATCAAG AGTTGGAGAC CCAACAACAG 
CATATCAGGA GAGTCCAGGA GAGACGGGAG TCCAGGGAAA GGGCCCTGCA GGACCAACTG 
GAGAGGGTGC AGGAGGCCTT GCAGACCAAA GTGCAAGACC TGGAAGCCCT CCAGCAGAAG 
GCACAATTAC AGCAGGAAAG CCAGGAAGAA CAGATGAGCA CTTTGCAAGC AGCCCTGGAA 
CAGGCCAGGG CAGCTCTGGA GGAGCATGAG GAGGCAATTG AGGCTCACAA GGAACAGAAC 
CAGAAAGTAC AGCAACAACA AACGGCTGCA GAGCTGCAGG TTCAAGCTTT GGAAGAGCTG 
CTAGGAGATC TCAGGGCTGA GTTTCAGGAG CAAGAGAAGG TTCTGGAGAC CCTGCGACAG 
CAGTGTACAG AGCAAGGACA TGAGCAGGAG ACACAGGCCT GGCACCAGCA CACAGAAGCC 
AGGCAGTGGG AAAAAACTAC AGCAGGCCAG GTCCAGGCCC TCAAGGAAGC CCTCAGAGTG 
GCCCAGGCCT CCCTTCAGAA GAAAGACCAG GAGCTCCTCC AGCAGGCAGA ACTAAGCCAG 
CGCTTGGAAG GCAGTGCAGC CGCTTTGCAG GTGGCCTTGG ACTCATGTCA GACTCAGGCC 
CGGCACCTGG AGGAGGCCCT GAGGGAGCGA GAGGAAGAGA TCAGGGAGCA GCAGCTACAG 
CACCAAGAAA TGGTACAGCA ACTGCAGCTG GCCCTGGCCC AGAGGGATGG GGAGCTGAGG 
CAACTCAAGG AAAAGGGGCA GCAGCAAGAG GCAGCCCTGA ACCAATCAGA CAAAGGTGAG 
GAAAAAGAAG AGAAGGTCCT GAGACAAAAC CTCAGGCATC TCCAGCTGAC CTTGTCCAGA 
AAGGAGTTCC AGCTGCAGGA GACCCACCGG AGGCATGGAC AGGAGTCTTA CTATGAAAGC 
CACAGCCCAG AGAAAATGGA GGATGGGGAG GAGGAGGAGG TCTATGGGAC AGGGACGATG 
GAGAAAATGT CCCTCCGTGA GATATCGCCT GTGTCCATGG AAGAGGCCAA GCTGCAAGAG 
GAGCTTGAGA GAGTGAAGAA TGTCCTGAGG CAAACAGAGG CTCGGGAAAT TGAGTGGCAA 
GAGAAGGCCC AAGCCTTGGC ATTCTCCCTA GCTGAGAGTG AAGCCAGCAT CTGTAACCTG 
CGTGAGGCTG CCCTATTCTT ACAGGCAAAG ACAGCAGAGC AAAACACAGA ACAGCTACAA 
CTTGAGGAGC AGTTGCAGCT CACAAGACAA GCTCTAGAGG ATGAGCAATC CCACAACCGG 
GGCTCAGCTT GGAGGACAGA GCCAGAGATC CAGGGAGATG AGGGTACTTC CCAAAGAAAG 
GCCACAGGAC CAGAGGATGA TGGATCTTGT ACAGAACTTG AGAAGACACA GCAATGGCAG 
CAAAGGCTAA AGTATCTGCT ACATGCTATG GCAAGTCTAG AGCATGAGAG GAACCGGCTT 
CAGCGACACA ACTTCCAATT GAGGGTCACC TTAGAGCAGG TAGAACGGGA GCGGAGGAAA 
CTGAAGAGGA ATTTTAACCG TATGACCCAT ACTACACCTC CCCTGCCTAG GGAGACCACA 
GCCCTCACAC CAGGCCAGAA GCAGGAGAAA GGGGGACAGG AGAATTCCTC AGAATTGAAA 
CATGTGGCTG AGTTACAGAG GCAGGTGACA GCCCTGCAGA CACAGCTGGC ACTGGAGCGG 
CAGCAGAAAC AGGACTACAT CTCCCGCTGT GTGCAGACAA GCCGAGAGCT AACAGATCTG 
CACCACAACC TTTCCAACTC ACTGTGGGCT GTAGCTCGGG CCCCTGAAGC CAACATCCTG 
GAGGCAGAGG CCCGAAAGCT AGATAAGTCA CTGACCCAGA GCCTGACATC TTCCGCACCA 
TCCCCACCCT GCTGCACTCC CAGGTCAGCC CAGGCTACCT CCCGTAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_007474240.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007474302.1 Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

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CloneID	OMc04275
Clone ID Related Accession (Same CDS sequence)	XM_007474241.2 , XM_007474240.2 , XM_016427979.1
Accession Version	XM_007474240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007474240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
AAGATGGATC AAATCAAGCA CAGGCAAGAA TTAGAGGCCA CATTAGAACA AATGAAAAGA 
GAGAAAGTGG ACCTGGAGGA GACCTGTGAG AAGAAACTAA GGGAACTGCA GGAGGAGTCA 
GAAGCTGCCC TGGCTCAGAG GATAAAGGAT CATGCCCAAG CTGAGAGCAA CAGATGTCAG 
ATGCAGCTAG AGATGGAAAA GGAAAGGATG TCACTACTGG AGACACTCTT GAAGACCCAG 
AATGAACTAG CTGATGCTGG CCATCAATTG GAGCAGCTGA GGCAGGACAT AAAGGACCAG 
AAGTTAAAGG AACAGGAGAA TATGGAGGAC CTTTGGGCCC AGCTGCAAGA GGCTCAGAAG 
ACACTGATGG AGACAAGAAG AAAGCATCAC AATGACCTTG CTGCCCTTGA GGAAGAGAAA 
AGCATCCTGC TACAGCAAAA AGAGAACCTG GAACAACAGG TACAAGACTT GAAGGCCCAA 
CTAGTCACAA GTGAAGATTC ATGTAGGCTG GCAGAGCATG AGGCCCAGAA GCATCTACAA 
GAGGTCCAGC AGTGTACTCA TATCCAGGAG GAGAGGGAGC AGGAAAAGGC CAGCCTGGTT 
CAGTCACTAG CAGAGAAGGA ACAAAGACTC CTCATTTTAC AAGAGGCTGA TTCACTCCAA 
CAGCAAGAGC TGAGTTCCTT GCGTCAGGAA GTGAAGGAGG CCCAGGAAGA ACAGGAGCAG 
CTCAGTGCCC AGGTACTGTT TCTAAAGGAA GAGATGCAGA AAAAAGAAGC TTCTCTTTTG 
GATCAAGAAA TCCTTTTGGA GAAGTTGGGA GCATCAAAGA AAACAGAGCA GCAGCTGAGA 
GCCTCACTGT GGGCACAAGA GGCCAAGGCA GCCCAGCTGC AACTCCAGCT GTGCAGTGTA 
GAAAGCCAAG TAGGGGCTCT AATATTAGAG CAGCAGAACG GACATCAGGC ACAGGCACAG 
CTAATGGGTC TCTACTCCAT TCTGCAGCAA GCCTTTGGAA GTGCTATTGA GAGTCGGCCA 
GAGCTGAGTG GTGCGGGTGA TGCTTCTCCC CTTTCCTGGA GCCCCAGAAC TTTGGAGCCA 
GGACAAGAGA ATTTCCTTAA AAACCAGATT GCTGTAACGA ACTTTACTAC TGAGGATGTG 
GCATCTTTAC TTCACAAGCT CCAGCGAGAC CTCTGGCAGG CTCGTCATAC TCGGGATGAT 
ATGAAATGTC AGGTTGAGAA GCTGGAGCAA CATCTGTCCC AAGCTGAGGC TGAGCGGAAC 
CAGTTTTCTA CGGAAATTCA AGAGCTTCAA AGTTTGCTCT CCCAGAATCA AAAAGAGAAA 
TTGAAATGGG AGGGAGAGCA GAGCTCTTTG GAGACAGAGT TGGCTGTACT TCAGGAAAGA 
GTGGCTTCCC TGCAGAACAG TCTGAAGAGA TCTGAGCAGC AAAGGACAGA AGCCCAGAAT 
GAGCAGGCAA CATTGTGGGC AGCCAATGAA AAATTGGTAT CAGAGGTGGA ACACCTTCAA 
GCATCAGTCG CAGTCACCAA GGCACAAGCT AATGCCAGGG AGGCCCTGGA AGCAGAACTG 
AGAGCAACAC ACTCAGCCCT GAGAATGAAA AATGAAGAAG CAGAGAGTGA GCGAGAGAAA 
GCAGAGGCCC TAAGGAAGAA GCTGGCCCAA GGGAAGGCCC TGCAGGAAAA TGTTGATCTC 
TTGACCAGTA CCCTTTCTAA GAGAGAGAAG GAATTAGAAG CCCTGCAGGG AGTCATTCAA 
GAACTGGAGA AACAGAGAGA ATCACAAATG GTGATGCAAG AACATCTGTC TCTGAACCTA 
AAGAAGAGAA GTCAAGAGCT GGAATCCCGG CAAGAACAGA TCCAAGAGCT GGAGAAACAG 
AAAGCTGGAC TAGAACATCT GCCTGTGGCC CTGGAACAGA AAAAGAAACA AATACGGTTA 
CAGAGAGAGC AGATTAAAGA GCTTGAGGGA AAGCAAGAAA CTCAGGGAAA CATCCTGGAA 
CATCAAGCTC TGGATTTACA GAAGAAAACA CAAACATTAG AGTCCCAGAG AGAACATATT 
CAAGATCTGG AAAACCAGTT GGTCACTCTG GAACATCTGG TTTTAGAAGT GAAAAAGAAT 
CATCAAGAAA TAGAGTCTCA GAAAAAAAAG ATTGAGGACT TAGAGAACCA GAGAGAAATG 
CAGAGGGTCA CTCTGACCCA TCTTACTTTG GACTTGGAGG AGAAAAGTCA GGAAATACAA 
TCTCAGAACA AAAAGATTAA AGAGTTAGAA AAGCACAATG CTCTCCTGAC AGTAAATCTG 
GAGGAGAGAT GCCAAGAGAT GAAGTCCTAC AGAGACCAGG TGGAAGAGTT AAAGAAGCAA 
AAGGATCAGC TTACTTGGGA CCTAGAGAAA AAAAACCAGG AGTTGGTCCT ACAAGAAGAG 
AAAATTCTGG TCCTGGAAGA TCAGAGAATG TTGCAGAACA GAGTATTAGA GGAAGACTTT 
GAACAGATGA AATTGGCCTT GAGGGAAAAA GATCAGGAAA TTGAGTCTCA AAGACAGCTC 
ATGATAGAAC GGGAAGAGGA AGAAAAGGGA CAAGCCAAGG CCCAACGCAG CAGCCTGGAG 
CATATGAAGT TGATCCTGAG GGACAAGGAG AAGGAGGTCG AGTACCAGCG AGAGAGGATT 
CACATGTTCC AACAGTATGG AGAACAGCGG GAAGAGCAAC TACAGGGCCT GCGGAGAAAG 
ATTGGGGAGA TGACGCTGCT CTTAACTCAG AAAGATCAAG AGTTGGAGAC CCAACAACAG 
CATATCAGGA GAGTCCAGGA GAGACGGGAG TCCAGGGAAA GGGCCCTGCA GGACCAACTG 
GAGAGGGTGC AGGAGGCCTT GCAGACCAAA GTGCAAGACC TGGAAGCCCT CCAGCAGAAG 
GCACAATTAC AGCAGGAAAG CCAGGAAGAA CAGATGAGCA CTTTGCAAGC AGCCCTGGAA 
CAGGCCAGGG CAGCTCTGGA GGAGCATGAG GAGGCAATTG AGGCTCACAA GGAACAGAAC 
CAGAAAGTAC AGCAACAACA AACGGCTGCA GAGCTGCAGG TTCAAGCTTT GGAAGAGCTG 
CTAGGAGATC TCAGGGCTGA GTTTCAGGAG CAAGAGAAGG TTCTGGAGAC CCTGCGACAG 
CAGTGTACAG AGCAAGGACA TGAGCAGGAG ACACAGGCCT GGCACCAGCA CACAGAAGCC 
AGGCAGTGGG AAAAAACTAC AGCAGGCCAG GTCCAGGCCC TCAAGGAAGC CCTCAGAGTG 
GCCCAGGCCT CCCTTCAGAA GAAAGACCAG GAGCTCCTCC AGCAGGCAGA ACTAAGCCAG 
CGCTTGGAAG GCAGTGCAGC CGCTTTGCAG GTGGCCTTGG ACTCATGTCA GACTCAGGCC 
CGGCACCTGG AGGAGGCCCT GAGGGAGCGA GAGGAAGAGA TCAGGGAGCA GCAGCTACAG 
CACCAAGAAA TGGTACAGCA ACTGCAGCTG GCCCTGGCCC AGAGGGATGG GGAGCTGAGG 
CAACTCAAGG AAAAGGGGCA GCAGCAAGAG GCAGCCCTGA ACCAATCAGA CAAAGGTGAG 
GAAAAAGAAG AGAAGGTCCT GAGACAAAAC CTCAGGCATC TCCAGCTGAC CTTGTCCAGA 
AAGGAGTTCC AGCTGCAGGA GACCCACCGG AGGCATGGAC AGGAGTCTTA CTATGAAAGC 
CACAGCCCAG AGAAAATGGA GGATGGGGAG GAGGAGGAGG TCTATGGGAC AGGGACGATG 
GAGAAAATGT CCCTCCGTGA GATATCGCCT GTGTCCATGG AAGAGGCCAA GCTGCAAGAG 
GAGCTTGAGA GAGTGAAGAA TGTCCTGAGG CAAACAGAGG CTCGGGAAAT TGAGTGGCAA 
GAGAAGGCCC AAGCCTTGGC ATTCTCCCTA GCTGAGAGTG AAGCCAGCAT CTGTAACCTG 
CGTGAGGCTG CCCTATTCTT ACAGGCAAAG ACAGCAGAGC AAAACACAGA ACAGCTACAA 
CTTGAGGAGC AGTTGCAGCT CACAAGACAA GCTCTAGAGG ATGAGCAATC CCACAACCGG 
GGCTCAGCTT GGAGGACAGA GCCAGAGATC CAGGGAGATG AGGGTACTTC CCAAAGAAAG 
GCCACAGGAC CAGAGGATGA TGGATCTTGT ACAGAACTTG AGAAGACACA GCAATGGCAG 
CAAAGGCTAA AGTATCTGCT ACATGCTATG GCAAGTCTAG AGCATGAGAG GAACCGGCTT 
CAGCGACACA ACTTCCAATT GAGGGTCACC TTAGAGCAGG TAGAACGGGA GCGGAGGAAA 
CTGAAGAGGA ATTTTAACCG TATGACCCAT ACTACACCTC CCCTGCCTAG GGAGACCACA 
GCCCTCACAC CAGGCCAGAA GCAGGAGAAA GGGGGACAGG AGAATTCCTC AGAATTGAAA 
CATGTGGCTG AGTTACAGAG GCAGGTGACA GCCCTGCAGA CACAGCTGGC ACTGGAGCGG 
CAGCAGAAAC AGGACTACAT CTCCCGCTGT GTGCAGACAA GCCGAGAGCT AACAGATCTG 
CACCACAACC TTTCCAACTC ACTGTGGGCT GTAGCTCGGG CCCCTGAAGC CAACATCCTG 
GAGGCAGAGG CCCGAAAGCT AGATAAGTCA CTGACCCAGA GCCTGACATC TTCCGCACCA 
TCCCCACCCT GCTGCACTCC CAGGTCAGCC CAGGCTACCT CCCGTAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007474302.1
CDS	364..7614
Translation	MEARDQGHNMKPQPLNMVLEKQILALQQQMAENQAASWHKLKISQEAQQRQAVLVRKLQAKVLQYRNWCQELEKRLEDMGGPMPKRWTSAEEPRLEQLLIRLEEEHQRSEGLVKVNTQLQEHLEEADVVNKALQEDVGKLTVDWSQAQDELARRQSDWQMEREFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKTATDRDLAELKAEQVKISGSLLTSCLHLSLELQSREAQGSGRLAGNEQVHLQLVAKTQELEKEIHKRSQELILLKARGNMEKTELQDRVTELSALLAQSKKQNEEQEKMLKALKDTMDILETNRSELMEHEASLSRNSQKEKLSLQQVIKDITQVVVDDEDSENQSFGLESSLEMESSIFCQLRSEDPDKAVTLVRSILAKKHQAVQGLRQQLSTCQEAVSFWQHQHTQWEEEGEALRQRLQKLTGERDVLAGQTQGLQGEVDSLNRDRGHLEKAREELQQQLEILEQEAWRLRRLNTELQLQGDLAQGEKVEQEEELQQAVRERDCLQETLKGLEARQSSSLSELISLKEALEKSHLEGELLNQERAEMARALAKAEQSIAELTGTERTLKAEVADLRDAAAKLSALNEALALDKVELNQQLLQLEQENQSVHGKVEASEKARSAVQMELDESEQRKEALLEEKACLEMQLQKSKDAEAELQGDLSREREEKIETQEKLNEAQRQLEVATTELEQLHQETTRQELLREKVIQEKEILVRDKAALEVHLKALERDQQDLSEQLLELSSAKELLETNLFEAQQQGSLMEITKEQLMMQIQTVTQAKEVIQGEVKCLQMELEAQRQQAEQEQDTAAKHRDQMEQESQAALQQQRATYEEELHLLQEERKMDQIKHRQELEATLEQMKREKVDLEETCEKKLRELQEESEAALAQRIKDHAQAESNRCQMQLEMEKERMSLLETLLKTQNELADAGHQLEQLRQDIKDQKLKEQENMEDLWAQLQEAQKTLMETRRKHHNDLAALEEEKSILLQQKENLEQQVQDLKAQLVTSEDSCRLAEHEAQKHLQEVQQCTHIQEEREQEKASLVQSLAEKEQRLLILQEADSLQQQELSSLRQEVKEAQEEQEQLSAQVLFLKEEMQKKEASLLDQEILLEKLGASKKTEQQLRASLWAQEAKAAQLQLQLCSVESQVGALILEQQNGHQAQAQLMGLYSILQQAFGSAIESRPELSGAGDASPLSWSPRTLEPGQENFLKNQIAVTNFTTEDVASLLHKLQRDLWQARHTRDDMKCQVEKLEQHLSQAEAERNQFSTEIQELQSLLSQNQKEKLKWEGEQSSLETELAVLQERVASLQNSLKRSEQQRTEAQNEQATLWAANEKLVSEVEHLQASVAVTKAQANAREALEAELRATHSALRMKNEEAESEREKAEALRKKLAQGKALQENVDLLTSTLSKREKELEALQGVIQELEKQRESQMVMQEHLSLNLKKRSQELESRQEQIQELEKQKAGLEHLPVALEQKKKQIRLQREQIKELEGKQETQGNILEHQALDLQKKTQTLESQREHIQDLENQLVTLEHLVLEVKKNHQEIESQKKKIEDLENQREMQRVTLTHLTLDLEEKSQEIQSQNKKIKELEKHNALLTVNLEERCQEMKSYRDQVEELKKQKDQLTWDLEKKNQELVLQEEKILVLEDQRMLQNRVLEEDFEQMKLALREKDQEIESQRQLMIEREEEEKGQAKAQRSSLEHMKLILRDKEKEVEYQRERIHMFQQYGEQREEQLQGLRRKIGEMTLLLTQKDQELETQQQHIRRVQERRESRERALQDQLERVQEALQTKVQDLEALQQKAQLQQESQEEQMSTLQAALEQARAALEEHEEAIEAHKEQNQKVQQQQTAAELQVQALEELLGDLRAEFQEQEKVLETLRQQCTEQGHEQETQAWHQHTEARQWEKTTAGQVQALKEALRVAQASLQKKDQELLQQAELSQRLEGSAAALQVALDSCQTQARHLEEALREREEEIREQQLQHQEMVQQLQLALAQRDGELRQLKEKGQQQEAALNQSDKGEEKEEKVLRQNLRHLQLTLSRKEFQLQETHRRHGQESYYESHSPEKMEDGEEEEVYGTGTMEKMSLREISPVSMEEAKLQEELERVKNVLRQTEAREIEWQEKAQALAFSLAESEASICNLREAALFLQAKTAEQNTEQLQLEEQLQLTRQALEDEQSHNRGSAWRTEPEIQGDEGTSQRKATGPEDDGSCTELEKTQQWQQRLKYLLHAMASLEHERNRLQRHNFQLRVTLEQVERERRKLKRNFNRMTHTTPPLPRETTALTPGQKQEKGGQENSSELKHVAELQRQVTALQTQLALERQQKQDYISRCVQTSRELTDLHHNLSNSLWAVARAPEANILEAEARKLDKSLTQSLTSSAPSPPCCTPRSAQATSRS

Target ORF information:

RefSeq Version	XM_007474240.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007474240.2

ORF Insert Sequence:

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
AAGATGGATC AAATCAAGCA CAGGCAAGAA TTAGAGGCCA CATTAGAACA AATGAAAAGA 
GAGAAAGTGG ACCTGGAGGA GACCTGTGAG AAGAAACTAA GGGAACTGCA GGAGGAGTCA 
GAAGCTGCCC TGGCTCAGAG GATAAAGGAT CATGCCCAAG CTGAGAGCAA CAGATGTCAG 
ATGCAGCTAG AGATGGAAAA GGAAAGGATG TCACTACTGG AGACACTCTT GAAGACCCAG 
AATGAACTAG CTGATGCTGG CCATCAATTG GAGCAGCTGA GGCAGGACAT AAAGGACCAG 
AAGTTAAAGG AACAGGAGAA TATGGAGGAC CTTTGGGCCC AGCTGCAAGA GGCTCAGAAG 
ACACTGATGG AGACAAGAAG AAAGCATCAC AATGACCTTG CTGCCCTTGA GGAAGAGAAA 
AGCATCCTGC TACAGCAAAA AGAGAACCTG GAACAACAGG TACAAGACTT GAAGGCCCAA 
CTAGTCACAA GTGAAGATTC ATGTAGGCTG GCAGAGCATG AGGCCCAGAA GCATCTACAA 
GAGGTCCAGC AGTGTACTCA TATCCAGGAG GAGAGGGAGC AGGAAAAGGC CAGCCTGGTT 
CAGTCACTAG CAGAGAAGGA ACAAAGACTC CTCATTTTAC AAGAGGCTGA TTCACTCCAA 
CAGCAAGAGC TGAGTTCCTT GCGTCAGGAA GTGAAGGAGG CCCAGGAAGA ACAGGAGCAG 
CTCAGTGCCC AGGTACTGTT TCTAAAGGAA GAGATGCAGA AAAAAGAAGC TTCTCTTTTG 
GATCAAGAAA TCCTTTTGGA GAAGTTGGGA GCATCAAAGA AAACAGAGCA GCAGCTGAGA 
GCCTCACTGT GGGCACAAGA GGCCAAGGCA GCCCAGCTGC AACTCCAGCT GTGCAGTGTA 
GAAAGCCAAG TAGGGGCTCT AATATTAGAG CAGCAGAACG GACATCAGGC ACAGGCACAG 
CTAATGGGTC TCTACTCCAT TCTGCAGCAA GCCTTTGGAA GTGCTATTGA GAGTCGGCCA 
GAGCTGAGTG GTGCGGGTGA TGCTTCTCCC CTTTCCTGGA GCCCCAGAAC TTTGGAGCCA 
GGACAAGAGA ATTTCCTTAA AAACCAGATT GCTGTAACGA ACTTTACTAC TGAGGATGTG 
GCATCTTTAC TTCACAAGCT CCAGCGAGAC CTCTGGCAGG CTCGTCATAC TCGGGATGAT 
ATGAAATGTC AGGTTGAGAA GCTGGAGCAA CATCTGTCCC AAGCTGAGGC TGAGCGGAAC 
CAGTTTTCTA CGGAAATTCA AGAGCTTCAA AGTTTGCTCT CCCAGAATCA AAAAGAGAAA 
TTGAAATGGG AGGGAGAGCA GAGCTCTTTG GAGACAGAGT TGGCTGTACT TCAGGAAAGA 
GTGGCTTCCC TGCAGAACAG TCTGAAGAGA TCTGAGCAGC AAAGGACAGA AGCCCAGAAT 
GAGCAGGCAA CATTGTGGGC AGCCAATGAA AAATTGGTAT CAGAGGTGGA ACACCTTCAA 
GCATCAGTCG CAGTCACCAA GGCACAAGCT AATGCCAGGG AGGCCCTGGA AGCAGAACTG 
AGAGCAACAC ACTCAGCCCT GAGAATGAAA AATGAAGAAG CAGAGAGTGA GCGAGAGAAA 
GCAGAGGCCC TAAGGAAGAA GCTGGCCCAA GGGAAGGCCC TGCAGGAAAA TGTTGATCTC 
TTGACCAGTA CCCTTTCTAA GAGAGAGAAG GAATTAGAAG CCCTGCAGGG AGTCATTCAA 
GAACTGGAGA AACAGAGAGA ATCACAAATG GTGATGCAAG AACATCTGTC TCTGAACCTA 
AAGAAGAGAA GTCAAGAGCT GGAATCCCGG CAAGAACAGA TCCAAGAGCT GGAGAAACAG 
AAAGCTGGAC TAGAACATCT GCCTGTGGCC CTGGAACAGA AAAAGAAACA AATACGGTTA 
CAGAGAGAGC AGATTAAAGA GCTTGAGGGA AAGCAAGAAA CTCAGGGAAA CATCCTGGAA 
CATCAAGCTC TGGATTTACA GAAGAAAACA CAAACATTAG AGTCCCAGAG AGAACATATT 
CAAGATCTGG AAAACCAGTT GGTCACTCTG GAACATCTGG TTTTAGAAGT GAAAAAGAAT 
CATCAAGAAA TAGAGTCTCA GAAAAAAAAG ATTGAGGACT TAGAGAACCA GAGAGAAATG 
CAGAGGGTCA CTCTGACCCA TCTTACTTTG GACTTGGAGG AGAAAAGTCA GGAAATACAA 
TCTCAGAACA AAAAGATTAA AGAGTTAGAA AAGCACAATG CTCTCCTGAC AGTAAATCTG 
GAGGAGAGAT GCCAAGAGAT GAAGTCCTAC AGAGACCAGG TGGAAGAGTT AAAGAAGCAA 
AAGGATCAGC TTACTTGGGA CCTAGAGAAA AAAAACCAGG AGTTGGTCCT ACAAGAAGAG 
AAAATTCTGG TCCTGGAAGA TCAGAGAATG TTGCAGAACA GAGTATTAGA GGAAGACTTT 
GAACAGATGA AATTGGCCTT GAGGGAAAAA GATCAGGAAA TTGAGTCTCA AAGACAGCTC 
ATGATAGAAC GGGAAGAGGA AGAAAAGGGA CAAGCCAAGG CCCAACGCAG CAGCCTGGAG 
CATATGAAGT TGATCCTGAG GGACAAGGAG AAGGAGGTCG AGTACCAGCG AGAGAGGATT 
CACATGTTCC AACAGTATGG AGAACAGCGG GAAGAGCAAC TACAGGGCCT GCGGAGAAAG 
ATTGGGGAGA TGACGCTGCT CTTAACTCAG AAAGATCAAG AGTTGGAGAC CCAACAACAG 
CATATCAGGA GAGTCCAGGA GAGACGGGAG TCCAGGGAAA GGGCCCTGCA GGACCAACTG 
GAGAGGGTGC AGGAGGCCTT GCAGACCAAA GTGCAAGACC TGGAAGCCCT CCAGCAGAAG 
GCACAATTAC AGCAGGAAAG CCAGGAAGAA CAGATGAGCA CTTTGCAAGC AGCCCTGGAA 
CAGGCCAGGG CAGCTCTGGA GGAGCATGAG GAGGCAATTG AGGCTCACAA GGAACAGAAC 
CAGAAAGTAC AGCAACAACA AACGGCTGCA GAGCTGCAGG TTCAAGCTTT GGAAGAGCTG 
CTAGGAGATC TCAGGGCTGA GTTTCAGGAG CAAGAGAAGG TTCTGGAGAC CCTGCGACAG 
CAGTGTACAG AGCAAGGACA TGAGCAGGAG ACACAGGCCT GGCACCAGCA CACAGAAGCC 
AGGCAGTGGG AAAAAACTAC AGCAGGCCAG GTCCAGGCCC TCAAGGAAGC CCTCAGAGTG 
GCCCAGGCCT CCCTTCAGAA GAAAGACCAG GAGCTCCTCC AGCAGGCAGA ACTAAGCCAG 
CGCTTGGAAG GCAGTGCAGC CGCTTTGCAG GTGGCCTTGG ACTCATGTCA GACTCAGGCC 
CGGCACCTGG AGGAGGCCCT GAGGGAGCGA GAGGAAGAGA TCAGGGAGCA GCAGCTACAG 
CACCAAGAAA TGGTACAGCA ACTGCAGCTG GCCCTGGCCC AGAGGGATGG GGAGCTGAGG 
CAACTCAAGG AAAAGGGGCA GCAGCAAGAG GCAGCCCTGA ACCAATCAGA CAAAGGTGAG 
GAAAAAGAAG AGAAGGTCCT GAGACAAAAC CTCAGGCATC TCCAGCTGAC CTTGTCCAGA 
AAGGAGTTCC AGCTGCAGGA GACCCACCGG AGGCATGGAC AGGAGTCTTA CTATGAAAGC 
CACAGCCCAG AGAAAATGGA GGATGGGGAG GAGGAGGAGG TCTATGGGAC AGGGACGATG 
GAGAAAATGT CCCTCCGTGA GATATCGCCT GTGTCCATGG AAGAGGCCAA GCTGCAAGAG 
GAGCTTGAGA GAGTGAAGAA TGTCCTGAGG CAAACAGAGG CTCGGGAAAT TGAGTGGCAA 
GAGAAGGCCC AAGCCTTGGC ATTCTCCCTA GCTGAGAGTG AAGCCAGCAT CTGTAACCTG 
CGTGAGGCTG CCCTATTCTT ACAGGCAAAG ACAGCAGAGC AAAACACAGA ACAGCTACAA 
CTTGAGGAGC AGTTGCAGCT CACAAGACAA GCTCTAGAGG ATGAGCAATC CCACAACCGG 
GGCTCAGCTT GGAGGACAGA GCCAGAGATC CAGGGAGATG AGGGTACTTC CCAAAGAAAG 
GCCACAGGAC CAGAGGATGA TGGATCTTGT ACAGAACTTG AGAAGACACA GCAATGGCAG 
CAAAGGCTAA AGTATCTGCT ACATGCTATG GCAAGTCTAG AGCATGAGAG GAACCGGCTT 
CAGCGACACA ACTTCCAATT GAGGGTCACC TTAGAGCAGG TAGAACGGGA GCGGAGGAAA 
CTGAAGAGGA ATTTTAACCG TATGACCCAT ACTACACCTC CCCTGCCTAG GGAGACCACA 
GCCCTCACAC CAGGCCAGAA GCAGGAGAAA GGGGGACAGG AGAATTCCTC AGAATTGAAA 
CATGTGGCTG AGTTACAGAG GCAGGTGACA GCCCTGCAGA CACAGCTGGC ACTGGAGCGG 
CAGCAGAAAC AGGACTACAT CTCCCGCTGT GTGCAGACAA GCCGAGAGCT AACAGATCTG 
CACCACAACC TTTCCAACTC ACTGTGGGCT GTAGCTCGGG CCCCTGAAGC CAACATCCTG 
GAGGCAGAGG CCCGAAAGCT AGATAAGTCA CTGACCCAGA GCCTGACATC TTCCGCACCA 
TCCCCACCCT GCTGCACTCC CAGGTCAGCC CAGGCTACCT CCCGTAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_016427979.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016283465.1 Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

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CloneID	OMc04275
Clone ID Related Accession (Same CDS sequence)	XM_007474241.2 , XM_007474240.2 , XM_016427979.1
Accession Version	XM_016427979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
AAGATGGATC AAATCAAGCA CAGGCAAGAA TTAGAGGCCA CATTAGAACA AATGAAAAGA 
GAGAAAGTGG ACCTGGAGGA GACCTGTGAG AAGAAACTAA GGGAACTGCA GGAGGAGTCA 
GAAGCTGCCC TGGCTCAGAG GATAAAGGAT CATGCCCAAG CTGAGAGCAA CAGATGTCAG 
ATGCAGCTAG AGATGGAAAA GGAAAGGATG TCACTACTGG AGACACTCTT GAAGACCCAG 
AATGAACTAG CTGATGCTGG CCATCAATTG GAGCAGCTGA GGCAGGACAT AAAGGACCAG 
AAGTTAAAGG AACAGGAGAA TATGGAGGAC CTTTGGGCCC AGCTGCAAGA GGCTCAGAAG 
ACACTGATGG AGACAAGAAG AAAGCATCAC AATGACCTTG CTGCCCTTGA GGAAGAGAAA 
AGCATCCTGC TACAGCAAAA AGAGAACCTG GAACAACAGG TACAAGACTT GAAGGCCCAA 
CTAGTCACAA GTGAAGATTC ATGTAGGCTG GCAGAGCATG AGGCCCAGAA GCATCTACAA 
GAGGTCCAGC AGTGTACTCA TATCCAGGAG GAGAGGGAGC AGGAAAAGGC CAGCCTGGTT 
CAGTCACTAG CAGAGAAGGA ACAAAGACTC CTCATTTTAC AAGAGGCTGA TTCACTCCAA 
CAGCAAGAGC TGAGTTCCTT GCGTCAGGAA GTGAAGGAGG CCCAGGAAGA ACAGGAGCAG 
CTCAGTGCCC AGGTACTGTT TCTAAAGGAA GAGATGCAGA AAAAAGAAGC TTCTCTTTTG 
GATCAAGAAA TCCTTTTGGA GAAGTTGGGA GCATCAAAGA AAACAGAGCA GCAGCTGAGA 
GCCTCACTGT GGGCACAAGA GGCCAAGGCA GCCCAGCTGC AACTCCAGCT GTGCAGTGTA 
GAAAGCCAAG TAGGGGCTCT AATATTAGAG CAGCAGAACG GACATCAGGC ACAGGCACAG 
CTAATGGGTC TCTACTCCAT TCTGCAGCAA GCCTTTGGAA GTGCTATTGA GAGTCGGCCA 
GAGCTGAGTG GTGCGGGTGA TGCTTCTCCC CTTTCCTGGA GCCCCAGAAC TTTGGAGCCA 
GGACAAGAGA ATTTCCTTAA AAACCAGATT GCTGTAACGA ACTTTACTAC TGAGGATGTG 
GCATCTTTAC TTCACAAGCT CCAGCGAGAC CTCTGGCAGG CTCGTCATAC TCGGGATGAT 
ATGAAATGTC AGGTTGAGAA GCTGGAGCAA CATCTGTCCC AAGCTGAGGC TGAGCGGAAC 
CAGTTTTCTA CGGAAATTCA AGAGCTTCAA AGTTTGCTCT CCCAGAATCA AAAAGAGAAA 
TTGAAATGGG AGGGAGAGCA GAGCTCTTTG GAGACAGAGT TGGCTGTACT TCAGGAAAGA 
GTGGCTTCCC TGCAGAACAG TCTGAAGAGA TCTGAGCAGC AAAGGACAGA AGCCCAGAAT 
GAGCAGGCAA CATTGTGGGC AGCCAATGAA AAATTGGTAT CAGAGGTGGA ACACCTTCAA 
GCATCAGTCG CAGTCACCAA GGCACAAGCT AATGCCAGGG AGGCCCTGGA AGCAGAACTG 
AGAGCAACAC ACTCAGCCCT GAGAATGAAA AATGAAGAAG CAGAGAGTGA GCGAGAGAAA 
GCAGAGGCCC TAAGGAAGAA GCTGGCCCAA GGGAAGGCCC TGCAGGAAAA TGTTGATCTC 
TTGACCAGTA CCCTTTCTAA GAGAGAGAAG GAATTAGAAG CCCTGCAGGG AGTCATTCAA 
GAACTGGAGA AACAGAGAGA ATCACAAATG GTGATGCAAG AACATCTGTC TCTGAACCTA 
AAGAAGAGAA GTCAAGAGCT GGAATCCCGG CAAGAACAGA TCCAAGAGCT GGAGAAACAG 
AAAGCTGGAC TAGAACATCT GCCTGTGGCC CTGGAACAGA AAAAGAAACA AATACGGTTA 
CAGAGAGAGC AGATTAAAGA GCTTGAGGGA AAGCAAGAAA CTCAGGGAAA CATCCTGGAA 
CATCAAGCTC TGGATTTACA GAAGAAAACA CAAACATTAG AGTCCCAGAG AGAACATATT 
CAAGATCTGG AAAACCAGTT GGTCACTCTG GAACATCTGG TTTTAGAAGT GAAAAAGAAT 
CATCAAGAAA TAGAGTCTCA GAAAAAAAAG ATTGAGGACT TAGAGAACCA GAGAGAAATG 
CAGAGGGTCA CTCTGACCCA TCTTACTTTG GACTTGGAGG AGAAAAGTCA GGAAATACAA 
TCTCAGAACA AAAAGATTAA AGAGTTAGAA AAGCACAATG CTCTCCTGAC AGTAAATCTG 
GAGGAGAGAT GCCAAGAGAT GAAGTCCTAC AGAGACCAGG TGGAAGAGTT AAAGAAGCAA 
AAGGATCAGC TTACTTGGGA CCTAGAGAAA AAAAACCAGG AGTTGGTCCT ACAAGAAGAG 
AAAATTCTGG TCCTGGAAGA TCAGAGAATG TTGCAGAACA GAGTATTAGA GGAAGACTTT 
GAACAGATGA AATTGGCCTT GAGGGAAAAA GATCAGGAAA TTGAGTCTCA AAGACAGCTC 
ATGATAGAAC GGGAAGAGGA AGAAAAGGGA CAAGCCAAGG CCCAACGCAG CAGCCTGGAG 
CATATGAAGT TGATCCTGAG GGACAAGGAG AAGGAGGTCG AGTACCAGCG AGAGAGGATT 
CACATGTTCC AACAGTATGG AGAACAGCGG GAAGAGCAAC TACAGGGCCT GCGGAGAAAG 
ATTGGGGAGA TGACGCTGCT CTTAACTCAG AAAGATCAAG AGTTGGAGAC CCAACAACAG 
CATATCAGGA GAGTCCAGGA GAGACGGGAG TCCAGGGAAA GGGCCCTGCA GGACCAACTG 
GAGAGGGTGC AGGAGGCCTT GCAGACCAAA GTGCAAGACC TGGAAGCCCT CCAGCAGAAG 
GCACAATTAC AGCAGGAAAG CCAGGAAGAA CAGATGAGCA CTTTGCAAGC AGCCCTGGAA 
CAGGCCAGGG CAGCTCTGGA GGAGCATGAG GAGGCAATTG AGGCTCACAA GGAACAGAAC 
CAGAAAGTAC AGCAACAACA AACGGCTGCA GAGCTGCAGG TTCAAGCTTT GGAAGAGCTG 
CTAGGAGATC TCAGGGCTGA GTTTCAGGAG CAAGAGAAGG TTCTGGAGAC CCTGCGACAG 
CAGTGTACAG AGCAAGGACA TGAGCAGGAG ACACAGGCCT GGCACCAGCA CACAGAAGCC 
AGGCAGTGGG AAAAAACTAC AGCAGGCCAG GTCCAGGCCC TCAAGGAAGC CCTCAGAGTG 
GCCCAGGCCT CCCTTCAGAA GAAAGACCAG GAGCTCCTCC AGCAGGCAGA ACTAAGCCAG 
CGCTTGGAAG GCAGTGCAGC CGCTTTGCAG GTGGCCTTGG ACTCATGTCA GACTCAGGCC 
CGGCACCTGG AGGAGGCCCT GAGGGAGCGA GAGGAAGAGA TCAGGGAGCA GCAGCTACAG 
CACCAAGAAA TGGTACAGCA ACTGCAGCTG GCCCTGGCCC AGAGGGATGG GGAGCTGAGG 
CAACTCAAGG AAAAGGGGCA GCAGCAAGAG GCAGCCCTGA ACCAATCAGA CAAAGGTGAG 
GAAAAAGAAG AGAAGGTCCT GAGACAAAAC CTCAGGCATC TCCAGCTGAC CTTGTCCAGA 
AAGGAGTTCC AGCTGCAGGA GACCCACCGG AGGCATGGAC AGGAGTCTTA CTATGAAAGC 
CACAGCCCAG AGAAAATGGA GGATGGGGAG GAGGAGGAGG TCTATGGGAC AGGGACGATG 
GAGAAAATGT CCCTCCGTGA GATATCGCCT GTGTCCATGG AAGAGGCCAA GCTGCAAGAG 
GAGCTTGAGA GAGTGAAGAA TGTCCTGAGG CAAACAGAGG CTCGGGAAAT TGAGTGGCAA 
GAGAAGGCCC AAGCCTTGGC ATTCTCCCTA GCTGAGAGTG AAGCCAGCAT CTGTAACCTG 
CGTGAGGCTG CCCTATTCTT ACAGGCAAAG ACAGCAGAGC AAAACACAGA ACAGCTACAA 
CTTGAGGAGC AGTTGCAGCT CACAAGACAA GCTCTAGAGG ATGAGCAATC CCACAACCGG 
GGCTCAGCTT GGAGGACAGA GCCAGAGATC CAGGGAGATG AGGGTACTTC CCAAAGAAAG 
GCCACAGGAC CAGAGGATGA TGGATCTTGT ACAGAACTTG AGAAGACACA GCAATGGCAG 
CAAAGGCTAA AGTATCTGCT ACATGCTATG GCAAGTCTAG AGCATGAGAG GAACCGGCTT 
CAGCGACACA ACTTCCAATT GAGGGTCACC TTAGAGCAGG TAGAACGGGA GCGGAGGAAA 
CTGAAGAGGA ATTTTAACCG TATGACCCAT ACTACACCTC CCCTGCCTAG GGAGACCACA 
GCCCTCACAC CAGGCCAGAA GCAGGAGAAA GGGGGACAGG AGAATTCCTC AGAATTGAAA 
CATGTGGCTG AGTTACAGAG GCAGGTGACA GCCCTGCAGA CACAGCTGGC ACTGGAGCGG 
CAGCAGAAAC AGGACTACAT CTCCCGCTGT GTGCAGACAA GCCGAGAGCT AACAGATCTG 
CACCACAACC TTTCCAACTC ACTGTGGGCT GTAGCTCGGG CCCCTGAAGC CAACATCCTG 
GAGGCAGAGG CCCGAAAGCT AGATAAGTCA CTGACCCAGA GCCTGACATC TTCCGCACCA 
TCCCCACCCT GCTGCACTCC CAGGTCAGCC CAGGCTACCT CCCGTAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016283465.1
CDS	419..7669
Translation	MEARDQGHNMKPQPLNMVLEKQILALQQQMAENQAASWHKLKISQEAQQRQAVLVRKLQAKVLQYRNWCQELEKRLEDMGGPMPKRWTSAEEPRLEQLLIRLEEEHQRSEGLVKVNTQLQEHLEEADVVNKALQEDVGKLTVDWSQAQDELARRQSDWQMEREFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKTATDRDLAELKAEQVKISGSLLTSCLHLSLELQSREAQGSGRLAGNEQVHLQLVAKTQELEKEIHKRSQELILLKARGNMEKTELQDRVTELSALLAQSKKQNEEQEKMLKALKDTMDILETNRSELMEHEASLSRNSQKEKLSLQQVIKDITQVVVDDEDSENQSFGLESSLEMESSIFCQLRSEDPDKAVTLVRSILAKKHQAVQGLRQQLSTCQEAVSFWQHQHTQWEEEGEALRQRLQKLTGERDVLAGQTQGLQGEVDSLNRDRGHLEKAREELQQQLEILEQEAWRLRRLNTELQLQGDLAQGEKVEQEEELQQAVRERDCLQETLKGLEARQSSSLSELISLKEALEKSHLEGELLNQERAEMARALAKAEQSIAELTGTERTLKAEVADLRDAAAKLSALNEALALDKVELNQQLLQLEQENQSVHGKVEASEKARSAVQMELDESEQRKEALLEEKACLEMQLQKSKDAEAELQGDLSREREEKIETQEKLNEAQRQLEVATTELEQLHQETTRQELLREKVIQEKEILVRDKAALEVHLKALERDQQDLSEQLLELSSAKELLETNLFEAQQQGSLMEITKEQLMMQIQTVTQAKEVIQGEVKCLQMELEAQRQQAEQEQDTAAKHRDQMEQESQAALQQQRATYEEELHLLQEERKMDQIKHRQELEATLEQMKREKVDLEETCEKKLRELQEESEAALAQRIKDHAQAESNRCQMQLEMEKERMSLLETLLKTQNELADAGHQLEQLRQDIKDQKLKEQENMEDLWAQLQEAQKTLMETRRKHHNDLAALEEEKSILLQQKENLEQQVQDLKAQLVTSEDSCRLAEHEAQKHLQEVQQCTHIQEEREQEKASLVQSLAEKEQRLLILQEADSLQQQELSSLRQEVKEAQEEQEQLSAQVLFLKEEMQKKEASLLDQEILLEKLGASKKTEQQLRASLWAQEAKAAQLQLQLCSVESQVGALILEQQNGHQAQAQLMGLYSILQQAFGSAIESRPELSGAGDASPLSWSPRTLEPGQENFLKNQIAVTNFTTEDVASLLHKLQRDLWQARHTRDDMKCQVEKLEQHLSQAEAERNQFSTEIQELQSLLSQNQKEKLKWEGEQSSLETELAVLQERVASLQNSLKRSEQQRTEAQNEQATLWAANEKLVSEVEHLQASVAVTKAQANAREALEAELRATHSALRMKNEEAESEREKAEALRKKLAQGKALQENVDLLTSTLSKREKELEALQGVIQELEKQRESQMVMQEHLSLNLKKRSQELESRQEQIQELEKQKAGLEHLPVALEQKKKQIRLQREQIKELEGKQETQGNILEHQALDLQKKTQTLESQREHIQDLENQLVTLEHLVLEVKKNHQEIESQKKKIEDLENQREMQRVTLTHLTLDLEEKSQEIQSQNKKIKELEKHNALLTVNLEERCQEMKSYRDQVEELKKQKDQLTWDLEKKNQELVLQEEKILVLEDQRMLQNRVLEEDFEQMKLALREKDQEIESQRQLMIEREEEEKGQAKAQRSSLEHMKLILRDKEKEVEYQRERIHMFQQYGEQREEQLQGLRRKIGEMTLLLTQKDQELETQQQHIRRVQERRESRERALQDQLERVQEALQTKVQDLEALQQKAQLQQESQEEQMSTLQAALEQARAALEEHEEAIEAHKEQNQKVQQQQTAAELQVQALEELLGDLRAEFQEQEKVLETLRQQCTEQGHEQETQAWHQHTEARQWEKTTAGQVQALKEALRVAQASLQKKDQELLQQAELSQRLEGSAAALQVALDSCQTQARHLEEALREREEEIREQQLQHQEMVQQLQLALAQRDGELRQLKEKGQQQEAALNQSDKGEEKEEKVLRQNLRHLQLTLSRKEFQLQETHRRHGQESYYESHSPEKMEDGEEEEVYGTGTMEKMSLREISPVSMEEAKLQEELERVKNVLRQTEAREIEWQEKAQALAFSLAESEASICNLREAALFLQAKTAEQNTEQLQLEEQLQLTRQALEDEQSHNRGSAWRTEPEIQGDEGTSQRKATGPEDDGSCTELEKTQQWQQRLKYLLHAMASLEHERNRLQRHNFQLRVTLEQVERERRKLKRNFNRMTHTTPPLPRETTALTPGQKQEKGGQENSSELKHVAELQRQVTALQTQLALERQQKQDYISRCVQTSRELTDLHHNLSNSLWAVARAPEANILEAEARKLDKSLTQSLTSSAPSPPCCTPRSAQATSRS

Target ORF information:

RefSeq Version	XM_016427979.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016427979.1

ORF Insert Sequence:

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
AAGATGGATC AAATCAAGCA CAGGCAAGAA TTAGAGGCCA CATTAGAACA AATGAAAAGA 
GAGAAAGTGG ACCTGGAGGA GACCTGTGAG AAGAAACTAA GGGAACTGCA GGAGGAGTCA 
GAAGCTGCCC TGGCTCAGAG GATAAAGGAT CATGCCCAAG CTGAGAGCAA CAGATGTCAG 
ATGCAGCTAG AGATGGAAAA GGAAAGGATG TCACTACTGG AGACACTCTT GAAGACCCAG 
AATGAACTAG CTGATGCTGG CCATCAATTG GAGCAGCTGA GGCAGGACAT AAAGGACCAG 
AAGTTAAAGG AACAGGAGAA TATGGAGGAC CTTTGGGCCC AGCTGCAAGA GGCTCAGAAG 
ACACTGATGG AGACAAGAAG AAAGCATCAC AATGACCTTG CTGCCCTTGA GGAAGAGAAA 
AGCATCCTGC TACAGCAAAA AGAGAACCTG GAACAACAGG TACAAGACTT GAAGGCCCAA 
CTAGTCACAA GTGAAGATTC ATGTAGGCTG GCAGAGCATG AGGCCCAGAA GCATCTACAA 
GAGGTCCAGC AGTGTACTCA TATCCAGGAG GAGAGGGAGC AGGAAAAGGC CAGCCTGGTT 
CAGTCACTAG CAGAGAAGGA ACAAAGACTC CTCATTTTAC AAGAGGCTGA TTCACTCCAA 
CAGCAAGAGC TGAGTTCCTT GCGTCAGGAA GTGAAGGAGG CCCAGGAAGA ACAGGAGCAG 
CTCAGTGCCC AGGTACTGTT TCTAAAGGAA GAGATGCAGA AAAAAGAAGC TTCTCTTTTG 
GATCAAGAAA TCCTTTTGGA GAAGTTGGGA GCATCAAAGA AAACAGAGCA GCAGCTGAGA 
GCCTCACTGT GGGCACAAGA GGCCAAGGCA GCCCAGCTGC AACTCCAGCT GTGCAGTGTA 
GAAAGCCAAG TAGGGGCTCT AATATTAGAG CAGCAGAACG GACATCAGGC ACAGGCACAG 
CTAATGGGTC TCTACTCCAT TCTGCAGCAA GCCTTTGGAA GTGCTATTGA GAGTCGGCCA 
GAGCTGAGTG GTGCGGGTGA TGCTTCTCCC CTTTCCTGGA GCCCCAGAAC TTTGGAGCCA 
GGACAAGAGA ATTTCCTTAA AAACCAGATT GCTGTAACGA ACTTTACTAC TGAGGATGTG 
GCATCTTTAC TTCACAAGCT CCAGCGAGAC CTCTGGCAGG CTCGTCATAC TCGGGATGAT 
ATGAAATGTC AGGTTGAGAA GCTGGAGCAA CATCTGTCCC AAGCTGAGGC TGAGCGGAAC 
CAGTTTTCTA CGGAAATTCA AGAGCTTCAA AGTTTGCTCT CCCAGAATCA AAAAGAGAAA 
TTGAAATGGG AGGGAGAGCA GAGCTCTTTG GAGACAGAGT TGGCTGTACT TCAGGAAAGA 
GTGGCTTCCC TGCAGAACAG TCTGAAGAGA TCTGAGCAGC AAAGGACAGA AGCCCAGAAT 
GAGCAGGCAA CATTGTGGGC AGCCAATGAA AAATTGGTAT CAGAGGTGGA ACACCTTCAA 
GCATCAGTCG CAGTCACCAA GGCACAAGCT AATGCCAGGG AGGCCCTGGA AGCAGAACTG 
AGAGCAACAC ACTCAGCCCT GAGAATGAAA AATGAAGAAG CAGAGAGTGA GCGAGAGAAA 
GCAGAGGCCC TAAGGAAGAA GCTGGCCCAA GGGAAGGCCC TGCAGGAAAA TGTTGATCTC 
TTGACCAGTA CCCTTTCTAA GAGAGAGAAG GAATTAGAAG CCCTGCAGGG AGTCATTCAA 
GAACTGGAGA AACAGAGAGA ATCACAAATG GTGATGCAAG AACATCTGTC TCTGAACCTA 
AAGAAGAGAA GTCAAGAGCT GGAATCCCGG CAAGAACAGA TCCAAGAGCT GGAGAAACAG 
AAAGCTGGAC TAGAACATCT GCCTGTGGCC CTGGAACAGA AAAAGAAACA AATACGGTTA 
CAGAGAGAGC AGATTAAAGA GCTTGAGGGA AAGCAAGAAA CTCAGGGAAA CATCCTGGAA 
CATCAAGCTC TGGATTTACA GAAGAAAACA CAAACATTAG AGTCCCAGAG AGAACATATT 
CAAGATCTGG AAAACCAGTT GGTCACTCTG GAACATCTGG TTTTAGAAGT GAAAAAGAAT 
CATCAAGAAA TAGAGTCTCA GAAAAAAAAG ATTGAGGACT TAGAGAACCA GAGAGAAATG 
CAGAGGGTCA CTCTGACCCA TCTTACTTTG GACTTGGAGG AGAAAAGTCA GGAAATACAA 
TCTCAGAACA AAAAGATTAA AGAGTTAGAA AAGCACAATG CTCTCCTGAC AGTAAATCTG 
GAGGAGAGAT GCCAAGAGAT GAAGTCCTAC AGAGACCAGG TGGAAGAGTT AAAGAAGCAA 
AAGGATCAGC TTACTTGGGA CCTAGAGAAA AAAAACCAGG AGTTGGTCCT ACAAGAAGAG 
AAAATTCTGG TCCTGGAAGA TCAGAGAATG TTGCAGAACA GAGTATTAGA GGAAGACTTT 
GAACAGATGA AATTGGCCTT GAGGGAAAAA GATCAGGAAA TTGAGTCTCA AAGACAGCTC 
ATGATAGAAC GGGAAGAGGA AGAAAAGGGA CAAGCCAAGG CCCAACGCAG CAGCCTGGAG 
CATATGAAGT TGATCCTGAG GGACAAGGAG AAGGAGGTCG AGTACCAGCG AGAGAGGATT 
CACATGTTCC AACAGTATGG AGAACAGCGG GAAGAGCAAC TACAGGGCCT GCGGAGAAAG 
ATTGGGGAGA TGACGCTGCT CTTAACTCAG AAAGATCAAG AGTTGGAGAC CCAACAACAG 
CATATCAGGA GAGTCCAGGA GAGACGGGAG TCCAGGGAAA GGGCCCTGCA GGACCAACTG 
GAGAGGGTGC AGGAGGCCTT GCAGACCAAA GTGCAAGACC TGGAAGCCCT CCAGCAGAAG 
GCACAATTAC AGCAGGAAAG CCAGGAAGAA CAGATGAGCA CTTTGCAAGC AGCCCTGGAA 
CAGGCCAGGG CAGCTCTGGA GGAGCATGAG GAGGCAATTG AGGCTCACAA GGAACAGAAC 
CAGAAAGTAC AGCAACAACA AACGGCTGCA GAGCTGCAGG TTCAAGCTTT GGAAGAGCTG 
CTAGGAGATC TCAGGGCTGA GTTTCAGGAG CAAGAGAAGG TTCTGGAGAC CCTGCGACAG 
CAGTGTACAG AGCAAGGACA TGAGCAGGAG ACACAGGCCT GGCACCAGCA CACAGAAGCC 
AGGCAGTGGG AAAAAACTAC AGCAGGCCAG GTCCAGGCCC TCAAGGAAGC CCTCAGAGTG 
GCCCAGGCCT CCCTTCAGAA GAAAGACCAG GAGCTCCTCC AGCAGGCAGA ACTAAGCCAG 
CGCTTGGAAG GCAGTGCAGC CGCTTTGCAG GTGGCCTTGG ACTCATGTCA GACTCAGGCC 
CGGCACCTGG AGGAGGCCCT GAGGGAGCGA GAGGAAGAGA TCAGGGAGCA GCAGCTACAG 
CACCAAGAAA TGGTACAGCA ACTGCAGCTG GCCCTGGCCC AGAGGGATGG GGAGCTGAGG 
CAACTCAAGG AAAAGGGGCA GCAGCAAGAG GCAGCCCTGA ACCAATCAGA CAAAGGTGAG 
GAAAAAGAAG AGAAGGTCCT GAGACAAAAC CTCAGGCATC TCCAGCTGAC CTTGTCCAGA 
AAGGAGTTCC AGCTGCAGGA GACCCACCGG AGGCATGGAC AGGAGTCTTA CTATGAAAGC 
CACAGCCCAG AGAAAATGGA GGATGGGGAG GAGGAGGAGG TCTATGGGAC AGGGACGATG 
GAGAAAATGT CCCTCCGTGA GATATCGCCT GTGTCCATGG AAGAGGCCAA GCTGCAAGAG 
GAGCTTGAGA GAGTGAAGAA TGTCCTGAGG CAAACAGAGG CTCGGGAAAT TGAGTGGCAA 
GAGAAGGCCC AAGCCTTGGC ATTCTCCCTA GCTGAGAGTG AAGCCAGCAT CTGTAACCTG 
CGTGAGGCTG CCCTATTCTT ACAGGCAAAG ACAGCAGAGC AAAACACAGA ACAGCTACAA 
CTTGAGGAGC AGTTGCAGCT CACAAGACAA GCTCTAGAGG ATGAGCAATC CCACAACCGG 
GGCTCAGCTT GGAGGACAGA GCCAGAGATC CAGGGAGATG AGGGTACTTC CCAAAGAAAG 
GCCACAGGAC CAGAGGATGA TGGATCTTGT ACAGAACTTG AGAAGACACA GCAATGGCAG 
CAAAGGCTAA AGTATCTGCT ACATGCTATG GCAAGTCTAG AGCATGAGAG GAACCGGCTT 
CAGCGACACA ACTTCCAATT GAGGGTCACC TTAGAGCAGG TAGAACGGGA GCGGAGGAAA 
CTGAAGAGGA ATTTTAACCG TATGACCCAT ACTACACCTC CCCTGCCTAG GGAGACCACA 
GCCCTCACAC CAGGCCAGAA GCAGGAGAAA GGGGGACAGG AGAATTCCTC AGAATTGAAA 
CATGTGGCTG AGTTACAGAG GCAGGTGACA GCCCTGCAGA CACAGCTGGC ACTGGAGCGG 
CAGCAGAAAC AGGACTACAT CTCCCGCTGT GTGCAGACAA GCCGAGAGCT AACAGATCTG 
CACCACAACC TTTCCAACTC ACTGTGGGCT GTAGCTCGGG CCCCTGAAGC CAACATCCTG 
GAGGCAGAGG CCCGAAAGCT AGATAAGTCA CTGACCCAGA GCCTGACATC TTCCGCACCA 
TCCCCACCCT GCTGCACTCC CAGGTCAGCC CAGGCTACCT CCCGTAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_007474242.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007474304.1 Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

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CloneID	OMc04276
Clone ID Related Accession (Same CDS sequence)	XM_007474242.2
Accession Version	XM_007474242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007474242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
ATGGATCAAA TCAAGCACAG GCAAGAATTA GAGGCCACAT TAGAACAAAT GAAAAGAGAG 
AAAGTGGACC TGGAGGAGAC CTGTGAGAAG AAACTAAGGG AACTGCAGGA GGAGTCAGAA 
GCTGCCCTGG CTCAGAGGAT AAAGGATCAT GCCCAAGCTG AGAGCAACAG ATGTCAGATG 
CAGCTAGAGA TGGAAAAGGA AAGGATGTCA CTACTGGAGA CACTCTTGAA GACCCAGAAT 
GAACTAGCTG ATGCTGGCCA TCAATTGGAG CAGCTGAGGC AGGACATAAA GGACCAGAAG 
TTAAAGGAAC AGGAGAATAT GGAGGACCTT TGGGCCCAGC TGCAAGAGGC TCAGAAGACA 
CTGATGGAGA CAAGAAGAAA GCATCACAAT GACCTTGCTG CCCTTGAGGA AGAGAAAAGC 
ATCCTGCTAC AGCAAAAAGA GAACCTGGAA CAACAGGTAC AAGACTTGAA GGCCCAACTA 
GTCACAAGTG AAGATTCATG TAGGCTGGCA GAGCATGAGG CCCAGAAGCA TCTACAAGAG 
GTCCAGCAGT GTACTCATAT CCAGGAGGAG AGGGAGCAGG AAAAGGCCAG CCTGGTTCAG 
TCACTAGCAG AGAAGGAACA AAGACTCCTC ATTTTACAAG AGGCTGATTC ACTCCAACAG 
CAAGAGCTGA GTTCCTTGCG TCAGGAAGTG AAGGAGGCCC AGGAAGAACA GGAGCAGCTC 
AGTGCCCAGG TACTGTTTCT AAAGGAAGAG ATGCAGAAAA AAGAAGCTTC TCTTTTGGAT 
CAAGAAATCC TTTTGGAGAA GTTGGGAGCA TCAAAGAAAA CAGAGCAGCA GCTGAGAGCC 
TCACTGTGGG CACAAGAGGC CAAGGCAGCC CAGCTGCAAC TCCAGCTGTG CAGTGTAGAA 
AGCCAAGTAG GGGCTCTAAT ATTAGAGCAG CAGAACGGAC ATCAGGCACA GGCACAGCTA 
ATGGGTCTCT ACTCCATTCT GCAGCAAGCC TTTGGAAGTG CTATTGAGAG TCGGCCAGAG 
CTGAGTGGTG CGGGTGATGC TTCTCCCCTT TCCTGGAGCC CCAGAACTTT GGAGCCAGGA 
CAAGAGAATT TCCTTAAAAA CCAGATTGCT GTAACGAACT TTACTACTGA GGATGTGGCA 
TCTTTACTTC ACAAGCTCCA GCGAGACCTC TGGCAGGCTC GTCATACTCG GGATGATATG 
AAATGTCAGG TTGAGAAGCT GGAGCAACAT CTGTCCCAAG CTGAGGCTGA GCGGAACCAG 
TTTTCTACGG AAATTCAAGA GCTTCAAAGT TTGCTCTCCC AGAATCAAAA AGAGAAATTG 
AAATGGGAGG GAGAGCAGAG CTCTTTGGAG ACAGAGTTGG CTGTACTTCA GGAAAGAGTG 
GCTTCCCTGC AGAACAGTCT GAAGAGATCT GAGCAGCAAA GGACAGAAGC CCAGAATGAG 
CAGGCAACAT TGTGGGCAGC CAATGAAAAA TTGGTATCAG AGGTGGAACA CCTTCAAGCA 
TCAGTCGCAG TCACCAAGGC ACAAGCTAAT GCCAGGGAGG CCCTGGAAGC AGAACTGAGA 
GCAACACACT CAGCCCTGAG AATGAAAAAT GAAGAAGCAG AGAGTGAGCG AGAGAAAGCA 
GAGGCCCTAA GGAAGAAGCT GGCCCAAGGG AAGGCCCTGC AGGAAAATGT TGATCTCTTG 
ACCAGTACCC TTTCTAAGAG AGAGAAGGAA TTAGAAGCCC TGCAGGGAGT CATTCAAGAA 
CTGGAGAAAC AGAGAGAATC ACAAATGGTG ATGCAAGAAC ATCTGTCTCT GAACCTAAAG 
AAGAGAAGTC AAGAGCTGGA ATCCCGGCAA GAACAGATCC AAGAGCTGGA GAAACAGAAA 
GCTGGACTAG AACATCTGCC TGTGGCCCTG GAACAGAAAA AGAAACAAAT ACGGTTACAG 
AGAGAGCAGA TTAAAGAGCT TGAGGGAAAG CAAGAAACTC AGGGAAACAT CCTGGAACAT 
CAAGCTCTGG ATTTACAGAA GAAAACACAA ACATTAGAGT CCCAGAGAGA ACATATTCAA 
GATCTGGAAA ACCAGTTGGT CACTCTGGAA CATCTGGTTT TAGAAGTGAA AAAGAATCAT 
CAAGAAATAG AGTCTCAGAA AAAAAAGATT GAGGACTTAG AGAACCAGAG AGAAATGCAG 
AGGGTCACTC TGACCCATCT TACTTTGGAC TTGGAGGAGA AAAGTCAGGA AATACAATCT 
CAGAACAAAA AGATTAAAGA GTTAGAAAAG CACAATGCTC TCCTGACAGT AAATCTGGAG 
GAGAGATGCC AAGAGATGAA GTCCTACAGA GACCAGGTGG AAGAGTTAAA GAAGCAAAAG 
GATCAGCTTA CTTGGGACCT AGAGAAAAAA AACCAGGAGT TGGTCCTACA AGAAGAGAAA 
ATTCTGGTCC TGGAAGATCA GAGAATGTTG CAGAACAGAG TATTAGAGGA AGACTTTGAA 
CAGATGAAAT TGGCCTTGAG GGAAAAAGAT CAGGAAATTG AGTCTCAAAG ACAGCTCATG 
ATAGAACGGG AAGAGGAAGA AAAGGGACAA GCCAAGGCCC AACGCAGCAG CCTGGAGCAT 
ATGAAGTTGA TCCTGAGGGA CAAGGAGAAG GAGGTCGAGT ACCAGCGAGA GAGGATTCAC 
ATGTTCCAAC AGTATGGAGA ACAGCGGGAA GAGCAACTAC AGGGCCTGCG GAGAAAGATT 
GGGGAGATGA CGCTGCTCTT AACTCAGAAA GATCAAGAGT TGGAGACCCA ACAACAGCAT 
ATCAGGAGAG TCCAGGAGAG ACGGGAGTCC AGGGAAAGGG CCCTGCAGGA CCAACTGGAG 
AGGGTGCAGG AGGCCTTGCA GACCAAAGTG CAAGACCTGG AAGCCCTCCA GCAGAAGGCA 
CAATTACAGC AGGAAAGCCA GGAAGAACAG ATGAGCACTT TGCAAGCAGC CCTGGAACAG 
GCCAGGGCAG CTCTGGAGGA GCATGAGGAG GCAATTGAGG CTCACAAGGA ACAGAACCAG 
AAAGTACAGC AACAACAAAC GGCTGCAGAG CTGCAGGTTC AAGCTTTGGA AGAGCTGCTA 
GGAGATCTCA GGGCTGAGTT TCAGGAGCAA GAGAAGGTTC TGGAGACCCT GCGACAGCAG 
TGTACAGAGC AAGGACATGA GCAGGAGACA CAGGCCTGGC ACCAGCACAC AGAAGCCAGG 
CAGTGGGAAA AAACTACAGC AGGCCAGGTC CAGGCCCTCA AGGAAGCCCT CAGAGTGGCC 
CAGGCCTCCC TTCAGAAGAA AGACCAGGAG CTCCTCCAGC AGGCAGAACT AAGCCAGCGC 
TTGGAAGGCA GTGCAGCCGC TTTGCAGGTG GCCTTGGACT CATGTCAGAC TCAGGCCCGG 
CACCTGGAGG AGGCCCTGAG GGAGCGAGAG GAAGAGATCA GGGAGCAGCA GCTACAGCAC 
CAAGAAATGG TACAGCAACT GCAGCTGGCC CTGGCCCAGA GGGATGGGGA GCTGAGGCAA 
CTCAAGGAAA AGGGGCAGCA GCAAGAGGCA GCCCTGAACC AATCAGACAA AGGTGAGGAA 
AAAGAAGAGA AGGTCCTGAG ACAAAACCTC AGGCATCTCC AGCTGACCTT GTCCAGAAAG 
GAGTTCCAGC TGCAGGAGAC CCACCGGAGG CATGGACAGG AGTCTTACTA TGAAAGCCAC 
AGCCCAGAGA AAATGGAGGA TGGGGAGGAG GAGGAGGTCT ATGGGACAGG GACGATGGAG 
AAAATGTCCC TCCGTGAGAT ATCGCCTGTG TCCATGGAAG AGGCCAAGCT GCAAGAGGAG 
CTTGAGAGAG TGAAGAATGT CCTGAGGCAA ACAGAGGCTC GGGAAATTGA GTGGCAAGAG 
AAGGCCCAAG CCTTGGCATT CTCCCTAGCT GAGAGTGAAG CCAGCATCTG TAACCTGCGT 
GAGGCTGCCC TATTCTTACA GGCAAAGACA GCAGAGCAAA ACACAGAACA GCTACAACTT 
GAGGAGCAGT TGCAGCTCAC AAGACAAGCT CTAGAGGATG AGCAATCCCA CAACCGGGGC 
TCAGCTTGGA GGACAGAGCC AGAGATCCAG GGAGATGAGG GTACTTCCCA AAGAAAGGCC 
ACAGGACCAG AGGATGATGG ATCTTGTACA GAACTTGAGA AGACACAGCA ATGGCAGCAA 
AGGCTAAAGT ATCTGCTACA TGCTATGGCA AGTCTAGAGC ATGAGAGGAA CCGGCTTCAG 
CGACACAACT TCCAATTGAG GGTCACCTTA GAGCAGGTAG AACGGGAGCG GAGGAAACTG 
AAGAGGAATT TTAACCGTAT GACCCATACT ACACCTCCCC TGCCTAGGGA GACCACAGCC 
CTCACACCAG GCCAGAAGCA GGAGAAAGGG GGACAGGAGA ATTCCTCAGA ATTGAAACAT 
GTGGCTGAGT TACAGAGGCA GGTGACAGCC CTGCAGACAC AGCTGGCACT GGAGCGGCAG 
CAGAAACAGG ACTACATCTC CCGCTGTGTG CAGACAAGCC GAGAGCTAAC AGATCTGCAC 
CACAACCTTT CCAACTCACT GTGGGCTGTA GCTCGGGCCC CTGAAGCCAA CATCCTGGAG 
GCAGAGGCCC GAAAGCTAGA TAAGTCACTG ACCCAGAGCC TGACATCTTC CGCACCATCC 
CCACCCTGCT GCACTCCCAG GTCAGCCCAG GCTACCTCCC GTAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007474304.1
CDS	364..7611
Translation	MEARDQGHNMKPQPLNMVLEKQILALQQQMAENQAASWHKLKISQEAQQRQAVLVRKLQAKVLQYRNWCQELEKRLEDMGGPMPKRWTSAEEPRLEQLLIRLEEEHQRSEGLVKVNTQLQEHLEEADVVNKALQEDVGKLTVDWSQAQDELARRQSDWQMEREFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKTATDRDLAELKAEQVKISGSLLTSCLHLSLELQSREAQGSGRLAGNEQVHLQLVAKTQELEKEIHKRSQELILLKARGNMEKTELQDRVTELSALLAQSKKQNEEQEKMLKALKDTMDILETNRSELMEHEASLSRNSQKEKLSLQQVIKDITQVVVDDEDSENQSFGLESSLEMESSIFCQLRSEDPDKAVTLVRSILAKKHQAVQGLRQQLSTCQEAVSFWQHQHTQWEEEGEALRQRLQKLTGERDVLAGQTQGLQGEVDSLNRDRGHLEKAREELQQQLEILEQEAWRLRRLNTELQLQGDLAQGEKVEQEEELQQAVRERDCLQETLKGLEARQSSSLSELISLKEALEKSHLEGELLNQERAEMARALAKAEQSIAELTGTERTLKAEVADLRDAAAKLSALNEALALDKVELNQQLLQLEQENQSVHGKVEASEKARSAVQMELDESEQRKEALLEEKACLEMQLQKSKDAEAELQGDLSREREEKIETQEKLNEAQRQLEVATTELEQLHQETTRQELLREKVIQEKEILVRDKAALEVHLKALERDQQDLSEQLLELSSAKELLETNLFEAQQQGSLMEITKEQLMMQIQTVTQAKEVIQGEVKCLQMELEAQRQQAEQEQDTAAKHRDQMEQESQAALQQQRATYEEELHLLQEERMDQIKHRQELEATLEQMKREKVDLEETCEKKLRELQEESEAALAQRIKDHAQAESNRCQMQLEMEKERMSLLETLLKTQNELADAGHQLEQLRQDIKDQKLKEQENMEDLWAQLQEAQKTLMETRRKHHNDLAALEEEKSILLQQKENLEQQVQDLKAQLVTSEDSCRLAEHEAQKHLQEVQQCTHIQEEREQEKASLVQSLAEKEQRLLILQEADSLQQQELSSLRQEVKEAQEEQEQLSAQVLFLKEEMQKKEASLLDQEILLEKLGASKKTEQQLRASLWAQEAKAAQLQLQLCSVESQVGALILEQQNGHQAQAQLMGLYSILQQAFGSAIESRPELSGAGDASPLSWSPRTLEPGQENFLKNQIAVTNFTTEDVASLLHKLQRDLWQARHTRDDMKCQVEKLEQHLSQAEAERNQFSTEIQELQSLLSQNQKEKLKWEGEQSSLETELAVLQERVASLQNSLKRSEQQRTEAQNEQATLWAANEKLVSEVEHLQASVAVTKAQANAREALEAELRATHSALRMKNEEAESEREKAEALRKKLAQGKALQENVDLLTSTLSKREKELEALQGVIQELEKQRESQMVMQEHLSLNLKKRSQELESRQEQIQELEKQKAGLEHLPVALEQKKKQIRLQREQIKELEGKQETQGNILEHQALDLQKKTQTLESQREHIQDLENQLVTLEHLVLEVKKNHQEIESQKKKIEDLENQREMQRVTLTHLTLDLEEKSQEIQSQNKKIKELEKHNALLTVNLEERCQEMKSYRDQVEELKKQKDQLTWDLEKKNQELVLQEEKILVLEDQRMLQNRVLEEDFEQMKLALREKDQEIESQRQLMIEREEEEKGQAKAQRSSLEHMKLILRDKEKEVEYQRERIHMFQQYGEQREEQLQGLRRKIGEMTLLLTQKDQELETQQQHIRRVQERRESRERALQDQLERVQEALQTKVQDLEALQQKAQLQQESQEEQMSTLQAALEQARAALEEHEEAIEAHKEQNQKVQQQQTAAELQVQALEELLGDLRAEFQEQEKVLETLRQQCTEQGHEQETQAWHQHTEARQWEKTTAGQVQALKEALRVAQASLQKKDQELLQQAELSQRLEGSAAALQVALDSCQTQARHLEEALREREEEIREQQLQHQEMVQQLQLALAQRDGELRQLKEKGQQQEAALNQSDKGEEKEEKVLRQNLRHLQLTLSRKEFQLQETHRRHGQESYYESHSPEKMEDGEEEEVYGTGTMEKMSLREISPVSMEEAKLQEELERVKNVLRQTEAREIEWQEKAQALAFSLAESEASICNLREAALFLQAKTAEQNTEQLQLEEQLQLTRQALEDEQSHNRGSAWRTEPEIQGDEGTSQRKATGPEDDGSCTELEKTQQWQQRLKYLLHAMASLEHERNRLQRHNFQLRVTLEQVERERRKLKRNFNRMTHTTPPLPRETTALTPGQKQEKGGQENSSELKHVAELQRQVTALQTQLALERQQKQDYISRCVQTSRELTDLHHNLSNSLWAVARAPEANILEAEARKLDKSLTQSLTSSAPSPPCCTPRSAQATSRS

Target ORF information:

RefSeq Version	XM_007474242.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007474242.2

ORF Insert Sequence:

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
ATGGATCAAA TCAAGCACAG GCAAGAATTA GAGGCCACAT TAGAACAAAT GAAAAGAGAG 
AAAGTGGACC TGGAGGAGAC CTGTGAGAAG AAACTAAGGG AACTGCAGGA GGAGTCAGAA 
GCTGCCCTGG CTCAGAGGAT AAAGGATCAT GCCCAAGCTG AGAGCAACAG ATGTCAGATG 
CAGCTAGAGA TGGAAAAGGA AAGGATGTCA CTACTGGAGA CACTCTTGAA GACCCAGAAT 
GAACTAGCTG ATGCTGGCCA TCAATTGGAG CAGCTGAGGC AGGACATAAA GGACCAGAAG 
TTAAAGGAAC AGGAGAATAT GGAGGACCTT TGGGCCCAGC TGCAAGAGGC TCAGAAGACA 
CTGATGGAGA CAAGAAGAAA GCATCACAAT GACCTTGCTG CCCTTGAGGA AGAGAAAAGC 
ATCCTGCTAC AGCAAAAAGA GAACCTGGAA CAACAGGTAC AAGACTTGAA GGCCCAACTA 
GTCACAAGTG AAGATTCATG TAGGCTGGCA GAGCATGAGG CCCAGAAGCA TCTACAAGAG 
GTCCAGCAGT GTACTCATAT CCAGGAGGAG AGGGAGCAGG AAAAGGCCAG CCTGGTTCAG 
TCACTAGCAG AGAAGGAACA AAGACTCCTC ATTTTACAAG AGGCTGATTC ACTCCAACAG 
CAAGAGCTGA GTTCCTTGCG TCAGGAAGTG AAGGAGGCCC AGGAAGAACA GGAGCAGCTC 
AGTGCCCAGG TACTGTTTCT AAAGGAAGAG ATGCAGAAAA AAGAAGCTTC TCTTTTGGAT 
CAAGAAATCC TTTTGGAGAA GTTGGGAGCA TCAAAGAAAA CAGAGCAGCA GCTGAGAGCC 
TCACTGTGGG CACAAGAGGC CAAGGCAGCC CAGCTGCAAC TCCAGCTGTG CAGTGTAGAA 
AGCCAAGTAG GGGCTCTAAT ATTAGAGCAG CAGAACGGAC ATCAGGCACA GGCACAGCTA 
ATGGGTCTCT ACTCCATTCT GCAGCAAGCC TTTGGAAGTG CTATTGAGAG TCGGCCAGAG 
CTGAGTGGTG CGGGTGATGC TTCTCCCCTT TCCTGGAGCC CCAGAACTTT GGAGCCAGGA 
CAAGAGAATT TCCTTAAAAA CCAGATTGCT GTAACGAACT TTACTACTGA GGATGTGGCA 
TCTTTACTTC ACAAGCTCCA GCGAGACCTC TGGCAGGCTC GTCATACTCG GGATGATATG 
AAATGTCAGG TTGAGAAGCT GGAGCAACAT CTGTCCCAAG CTGAGGCTGA GCGGAACCAG 
TTTTCTACGG AAATTCAAGA GCTTCAAAGT TTGCTCTCCC AGAATCAAAA AGAGAAATTG 
AAATGGGAGG GAGAGCAGAG CTCTTTGGAG ACAGAGTTGG CTGTACTTCA GGAAAGAGTG 
GCTTCCCTGC AGAACAGTCT GAAGAGATCT GAGCAGCAAA GGACAGAAGC CCAGAATGAG 
CAGGCAACAT TGTGGGCAGC CAATGAAAAA TTGGTATCAG AGGTGGAACA CCTTCAAGCA 
TCAGTCGCAG TCACCAAGGC ACAAGCTAAT GCCAGGGAGG CCCTGGAAGC AGAACTGAGA 
GCAACACACT CAGCCCTGAG AATGAAAAAT GAAGAAGCAG AGAGTGAGCG AGAGAAAGCA 
GAGGCCCTAA GGAAGAAGCT GGCCCAAGGG AAGGCCCTGC AGGAAAATGT TGATCTCTTG 
ACCAGTACCC TTTCTAAGAG AGAGAAGGAA TTAGAAGCCC TGCAGGGAGT CATTCAAGAA 
CTGGAGAAAC AGAGAGAATC ACAAATGGTG ATGCAAGAAC ATCTGTCTCT GAACCTAAAG 
AAGAGAAGTC AAGAGCTGGA ATCCCGGCAA GAACAGATCC AAGAGCTGGA GAAACAGAAA 
GCTGGACTAG AACATCTGCC TGTGGCCCTG GAACAGAAAA AGAAACAAAT ACGGTTACAG 
AGAGAGCAGA TTAAAGAGCT TGAGGGAAAG CAAGAAACTC AGGGAAACAT CCTGGAACAT 
CAAGCTCTGG ATTTACAGAA GAAAACACAA ACATTAGAGT CCCAGAGAGA ACATATTCAA 
GATCTGGAAA ACCAGTTGGT CACTCTGGAA CATCTGGTTT TAGAAGTGAA AAAGAATCAT 
CAAGAAATAG AGTCTCAGAA AAAAAAGATT GAGGACTTAG AGAACCAGAG AGAAATGCAG 
AGGGTCACTC TGACCCATCT TACTTTGGAC TTGGAGGAGA AAAGTCAGGA AATACAATCT 
CAGAACAAAA AGATTAAAGA GTTAGAAAAG CACAATGCTC TCCTGACAGT AAATCTGGAG 
GAGAGATGCC AAGAGATGAA GTCCTACAGA GACCAGGTGG AAGAGTTAAA GAAGCAAAAG 
GATCAGCTTA CTTGGGACCT AGAGAAAAAA AACCAGGAGT TGGTCCTACA AGAAGAGAAA 
ATTCTGGTCC TGGAAGATCA GAGAATGTTG CAGAACAGAG TATTAGAGGA AGACTTTGAA 
CAGATGAAAT TGGCCTTGAG GGAAAAAGAT CAGGAAATTG AGTCTCAAAG ACAGCTCATG 
ATAGAACGGG AAGAGGAAGA AAAGGGACAA GCCAAGGCCC AACGCAGCAG CCTGGAGCAT 
ATGAAGTTGA TCCTGAGGGA CAAGGAGAAG GAGGTCGAGT ACCAGCGAGA GAGGATTCAC 
ATGTTCCAAC AGTATGGAGA ACAGCGGGAA GAGCAACTAC AGGGCCTGCG GAGAAAGATT 
GGGGAGATGA CGCTGCTCTT AACTCAGAAA GATCAAGAGT TGGAGACCCA ACAACAGCAT 
ATCAGGAGAG TCCAGGAGAG ACGGGAGTCC AGGGAAAGGG CCCTGCAGGA CCAACTGGAG 
AGGGTGCAGG AGGCCTTGCA GACCAAAGTG CAAGACCTGG AAGCCCTCCA GCAGAAGGCA 
CAATTACAGC AGGAAAGCCA GGAAGAACAG ATGAGCACTT TGCAAGCAGC CCTGGAACAG 
GCCAGGGCAG CTCTGGAGGA GCATGAGGAG GCAATTGAGG CTCACAAGGA ACAGAACCAG 
AAAGTACAGC AACAACAAAC GGCTGCAGAG CTGCAGGTTC AAGCTTTGGA AGAGCTGCTA 
GGAGATCTCA GGGCTGAGTT TCAGGAGCAA GAGAAGGTTC TGGAGACCCT GCGACAGCAG 
TGTACAGAGC AAGGACATGA GCAGGAGACA CAGGCCTGGC ACCAGCACAC AGAAGCCAGG 
CAGTGGGAAA AAACTACAGC AGGCCAGGTC CAGGCCCTCA AGGAAGCCCT CAGAGTGGCC 
CAGGCCTCCC TTCAGAAGAA AGACCAGGAG CTCCTCCAGC AGGCAGAACT AAGCCAGCGC 
TTGGAAGGCA GTGCAGCCGC TTTGCAGGTG GCCTTGGACT CATGTCAGAC TCAGGCCCGG 
CACCTGGAGG AGGCCCTGAG GGAGCGAGAG GAAGAGATCA GGGAGCAGCA GCTACAGCAC 
CAAGAAATGG TACAGCAACT GCAGCTGGCC CTGGCCCAGA GGGATGGGGA GCTGAGGCAA 
CTCAAGGAAA AGGGGCAGCA GCAAGAGGCA GCCCTGAACC AATCAGACAA AGGTGAGGAA 
AAAGAAGAGA AGGTCCTGAG ACAAAACCTC AGGCATCTCC AGCTGACCTT GTCCAGAAAG 
GAGTTCCAGC TGCAGGAGAC CCACCGGAGG CATGGACAGG AGTCTTACTA TGAAAGCCAC 
AGCCCAGAGA AAATGGAGGA TGGGGAGGAG GAGGAGGTCT ATGGGACAGG GACGATGGAG 
AAAATGTCCC TCCGTGAGAT ATCGCCTGTG TCCATGGAAG AGGCCAAGCT GCAAGAGGAG 
CTTGAGAGAG TGAAGAATGT CCTGAGGCAA ACAGAGGCTC GGGAAATTGA GTGGCAAGAG 
AAGGCCCAAG CCTTGGCATT CTCCCTAGCT GAGAGTGAAG CCAGCATCTG TAACCTGCGT 
GAGGCTGCCC TATTCTTACA GGCAAAGACA GCAGAGCAAA ACACAGAACA GCTACAACTT 
GAGGAGCAGT TGCAGCTCAC AAGACAAGCT CTAGAGGATG AGCAATCCCA CAACCGGGGC 
TCAGCTTGGA GGACAGAGCC AGAGATCCAG GGAGATGAGG GTACTTCCCA AAGAAAGGCC 
ACAGGACCAG AGGATGATGG ATCTTGTACA GAACTTGAGA AGACACAGCA ATGGCAGCAA 
AGGCTAAAGT ATCTGCTACA TGCTATGGCA AGTCTAGAGC ATGAGAGGAA CCGGCTTCAG 
CGACACAACT TCCAATTGAG GGTCACCTTA GAGCAGGTAG AACGGGAGCG GAGGAAACTG 
AAGAGGAATT TTAACCGTAT GACCCATACT ACACCTCCCC TGCCTAGGGA GACCACAGCC 
CTCACACCAG GCCAGAAGCA GGAGAAAGGG GGACAGGAGA ATTCCTCAGA ATTGAAACAT 
GTGGCTGAGT TACAGAGGCA GGTGACAGCC CTGCAGACAC AGCTGGCACT GGAGCGGCAG 
CAGAAACAGG ACTACATCTC CCGCTGTGTG CAGACAAGCC GAGAGCTAAC AGATCTGCAC 
CACAACCTTT CCAACTCACT GTGGGCTGTA GCTCGGGCCC CTGAAGCCAA CATCCTGGAG 
GCAGAGGCCC GAAAGCTAGA TAAGTCACTG ACCCAGAGCC TGACATCTTC CGCACCATCC 
CCACCCTGCT GCACTCCCAG GTCAGCCCAG GCTACCTCCC GTAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_007474243.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007474305.1 Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

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CloneID	OMc04277
Clone ID Related Accession (Same CDS sequence)	XM_007474243.2
Accession Version	XM_007474243.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007474243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
AAGATGGATC AAATCAAGCA CAGGCAAGAA TTAGAGGCCA CATTAGAACA AATGAAAAGA 
GAGAAAGTGG ACCTGGAGGA GACCTGTGAG AAGAAACTAA GGGAACTGCA GGAGGAGTCA 
GAAGCTGCCC TGGCTCAGAG GATAAAGGAT CATGCCCAAG CTGAGAGCAA CAGATGTCAG 
ATGCAGCTAG AGATGGAAAA GGAAAGGATG TCACTACTGG AGACACTCTT GAAGACCCAG 
AATGAACTAG CTGATGCTGG CCATCAATTG GAGCAGCTGA GGCAGGACAT AAAGGACCAG 
AAGTTAAAGG AACAGGAGAA TATGGAGGAC CTTTGGGCCC AGCTGCAAGA GGCTCAGAAG 
ACACTGATGG AGACAAGAAG AAAGCATCAC AATGACCTTG CTGCCCTTGA GGAAGAGAAA 
AGCATCCTGC TACAGCAAAA AGAGAACCTG GAACAACAGG TACAAGACTT GAAGGCCCAA 
CTAGTCACAA GTGAAGATTC ATGTAGGCTG GCAGAGCATG AGGCCCAGAA GCATCTACAA 
GAGGTCCAGC AGTGTACTCA TATCCAGGAG GAGAGGGAGC AGGAAAAGGC CAGCCTGGTT 
CAGTCACTAG CAGAGAAGGA ACAAAGACTC CTCATTTTAC AAGAGGCTGA TTCACTCCAA 
CAGCAAGAGC TGAGTTCCTT GCGTCAGGAA GTGAAGGAGG CCCAGGAAGA ACAGGAGCAG 
CTCAGTGCCC AGGTACTGTT TCTAAAGGAA GAGATGCAGA AAAAAGAAGC TTCTCTTTTG 
GATCAAGAAA TCCTTTTGGA GAAGTTGGGA GCATCAAAGA AAACAGAGCA GCAGCTGAGA 
GCCTCACTGT GGGCACAAGA GGCCAAGGCA GCCCAGCTGC AACTCCAGCT GTGCAGTGTA 
GAAAGCCAAG TAGGGGCTCT AATATTAGAG CAGCAGAACG GACATCAGGC ACAGGCACAG 
CTAATGGGTC TCTACTCCAT TCTGCAGCAA GCCTTTGGAA GTGCTATTGA GAGTCGGCCA 
GAGCTGAGTG GTGCGGGTGA TGCTTCTCCC CTTTCCTGGA GCCCCAGAAC TTTGGAGCCA 
GGACAAGAGA ATTTCCTTAA AAACCAGATT GCTGTAACGA ACTTTACTAC TGAGGATGTG 
GCATCTTTAC TTCACAAGCT CCAGCGAGAC CTCTGGCAGG CTCGTCATAC TCGGGATGAT 
ATGAAATGTC AGGTTGAGAA GCTGGAGCAA CATCTGTCCC AAGCTGAGGC TGAGCGGAAC 
CAGTTTTCTA CGGAAATTCA AGAGCTTCAA AGTTTGCTCT CCCAGAATCA AAAAGAGAAA 
TTGAAATGGG AGGGAGAGCA GAGCTCTTTG GAGACAGAGT TGGCTGTACT TCAGGAAAGA 
GTGGCTTCCC TGCAGAACAG TCTGAAGAGA TCTGAGCAGC AAAGGACAGA AGCCCAGAAT 
GAGCAGGCAA CATTGTGGGC AGCCAATGAA AAATTGGTAT CAGAGGTGGA ACACCTTCAA 
GCATCAGTCG CAGTCACCAA GGCACAAGCT AATGCCAGGG AGGCCCTGGA AGCAGAACTG 
AGAGCAACAC ACTCAGCCCT GAGAATGAAA AATGAAGAAG CAGAGAGTGA GCGAGAGAAA 
GCAGAGGCCC TAAGGAAGAA GCTGGCCCAA GGGAAGGCCC TGCAGGAAAA TGTTGATCTC 
TTGACCAGTA CCCTTTCTAA GAGAGAGAAG GAATTAGAAG CCCTGCAGGG AGTCATTCAA 
GAACTGGAGA AACAGAGAGA ATCACAAATG GTGATGCAAG AACATCTGTC TCTGAACCTA 
AAGAAGAGAA GTCAAGAGCT GGAATCCCGG CAAGAACAGA TCCAAGAGCT GGAGAAACAG 
AAAGCTGGAC TAGAACATCT GCCTGTGGCC CTGGAACAGA AAAAGAAACA AATACGGTTA 
CAGAGAGAGC AGATTAAAGA GCTTGAGGGA AAGCAAGAAA CTCAGGGAAA CATCCTGGAA 
CATCAAGCTC TGGATTTACA GAAGAAAACA CAAACATTAG AGTCCCAGAG AGAACATATT 
CAAGATCTGG AAAACCAGTT GGTCACTCTG GAACATCTGG TTTTAGAAGT GAAAAAGAAT 
CATCAAGAAA TAGAGTCTCA GAAAAAAAAG ATTGAGGACT TAGAGAACCA GAGAGAAATG 
CAGAGGGTCA CTCTGACCCA TCTTACTTTG GACTTGGAGG AGAAAAGTCA GGAAATACAA 
TCTCAGAACA AAAAGATTAA AGAGTTAGAA AAGCACAATG CTCTCCTGAC AGTAAATCTG 
GAGGAGAGAT GCCAAGAGAT GAAGTCCTAC AGAGACCAGG TGGAAGAGTT AAAGAAGCAA 
AAGGATCAGC TTACTTGGGA CCTAGAGAAA AAAAACCAGG AGTTGGTCCT ACAAGAAGAG 
AAAATTCTGG TCCTGGAAGA TCAGAGAATG TTGCAGAACA GAGTATTAGA GGAAGACTTT 
GAACAGATGA AATTGGCCTT GAGGGAAAAA GATCAGGAAA TTGAGTCTCA AAGACAGCTC 
ATGATAGAAC GGGAAGAGGA AGAAAAGGGA CAAGCCAAGG CCCAACGCAG CAGCCTGGAG 
CATATGAAGT TGATCCTGAG GGACAAGGAG AAGGAGGTCG AGTACCAGCG AGAGAGGATT 
CACATGTTCC AACAGTATGG AGAACAGCGG GAAGAGCAAC TACAGGGCCT GCGGAGAAAG 
ATTGGGGAGA TGACGCTGCT CTTAACTCAG AAAGATCAAG AGTTGGAGAC CCAACAACAG 
CATATCAGGA GAGTCCAGGA GAGACGGGAG TCCAGGGAAA GGGCCCTGCA GGACCAACTG 
GAGAGGGTGC AGGAGGCCTT GCAGACCAAA GTGCAAGACC TGGAAGCCCT CCAGCAGAAG 
GCACAATTAC AGCAGGAAAG CCAGGAAGAA CAGATGAGCA CTTTGCAAGC AGCCCTGGAA 
CAGGCCAGGG CAGCTCTGGA GGAGCATGAG GAGGCAATTG AGGCTCACAA GGAACAGAAC 
CAGAAAGTAC AGCAACAACA AACGGCTGCA GAGCTGCAGG TTCAAGCTTT GGAAGAGCTG 
CTAGGAGATC TCAGGGCTGA GTTTCAGGAG CAAGAGAAGG TTCTGGAGAC CCTGCGACAG 
CAGTGTACAG AGCAAGGACA TGAGCAGGAG ACACAGGCCT GGCACCAGCA CACAGAAGCC 
AGGCAGTGGG AAAAAACTAC AGCAGGCCAG GTCCAGGCCC TCAAGGAAGC CCTCAGAGTG 
GCCCAGGCCT CCCTTCAGAA GAAAGACCAG GAGCTCCTCC AGCAGGCAGA ACTAAGCCAG 
CGCTTGGAAG GCAGTGCAGC CGCTTTGCAG GTGGCCTTGG ACTCATGTCA GACTCAGGCC 
CGGCACCTGG AGGAGGCCCT GAGGGAGCGA GAGGAAGAGA TCAGGGAGCA GCAGCTACAG 
CACCAAGAAA TGGTACAGCA ACTGCAGCTG GCCCTGGCCC AGAGGGATGG GGAGCTGAGG 
CAACTCAAGG AAAAGGGGCA GCAGCAAGAG GCAGCCCTGA ACCAATCAGA CAAAGGTGAG 
GAAAAAGAAG AGAAGGTCCT GAGACAAAAC CTCAGGCATC TCCAGCTGAC CTTGTCCAGA 
AAGGAGTTCC AGCTGCAGGA GACCCACCGG AGGCATGGAC AGGAGTCTTA CTATGAAAGC 
CACAGCCCAG AGAAAATGGA GGATGGGGAG GAGGAGGAGG TCTATGGGAC AGGGACGATG 
GAGAAAATGT CCCTCCGTGA GATATCGCCT GTGTCCATGG AAGAGGCCAA GCTGCAAGAG 
GAGCTTGAGA GAGTGAAGAA TGTCCTGAGG CAAACAGAGG CTCGGGAAAT TGAGTGGCAA 
GAGAAGGCCC AAGCCTTGGC ATTCTCCCTA GCTGAGAGTG AAGCCAGCAT CTGTAACCTG 
CGTGAGGCTG CCCTATTCTT ACAGGCAAAG ACAGCAGAGC AAAACACAGA ACAGCTACAA 
CTTGAGGAGC AGTTGCAGCT CACAAGACAA GCTCTAGAGG ATGAGCAATC CCACAACCGG 
GGCTCAGCTT GGAGGACAGA GCCAGAGATC CAGGGAGATG AGGGTACTTC CCAAAGAAAG 
GCCACAGGAC CAGAGGATGA TGGATCTTGT ACAGAACTTG AGAAGACACA GCAATGGCAG 
CAAAGGCTAA AGTATCTGCT ACATGCTATG GCAAGTCTAG AGCATGAGAG GAACCGGCTT 
CAGCGACACA ACTTCCAATT GAGGGTCACC TTAGAGCAGG TAGAACGGGA GCGGAGGAAA 
CTGAAGAGGA ATTTTAACCG TATGACCCAT ACTACACCTC CCCTGCCTAG GGAGACCACA 
GCCCTCACAC CAGGCCAGAA GGAGAAAGGG GGACAGGAGA ATTCCTCAGA ATTGAAACAT 
GTGGCTGAGT TACAGAGGCA GGTGACAGCC CTGCAGACAC AGCTGGCACT GGAGCGGCAG 
CAGAAACAGG ACTACATCTC CCGCTGTGTG CAGACAAGCC GAGAGCTAAC AGATCTGCAC 
CACAACCTTT CCAACTCACT GTGGGCTGTA GCTCGGGCCC CTGAAGCCAA CATCCTGGAG 
GCAGAGGCCC GAAAGCTAGA TAAGTCACTG ACCCAGAGCC TGACATCTTC CGCACCATCC 
CCACCCTGCT GCACTCCCAG GTCAGCCCAG GCTACCTCCC GTAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007474305.1
CDS	365..7612
Translation	MEARDQGHNMKPQPLNMVLEKQILALQQQMAENQAASWHKLKISQEAQQRQAVLVRKLQAKVLQYRNWCQELEKRLEDMGGPMPKRWTSAEEPRLEQLLIRLEEEHQRSEGLVKVNTQLQEHLEEADVVNKALQEDVGKLTVDWSQAQDELARRQSDWQMEREFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKTATDRDLAELKAEQVKISGSLLTSCLHLSLELQSREAQGSGRLAGNEQVHLQLVAKTQELEKEIHKRSQELILLKARGNMEKTELQDRVTELSALLAQSKKQNEEQEKMLKALKDTMDILETNRSELMEHEASLSRNSQKEKLSLQQVIKDITQVVVDDEDSENQSFGLESSLEMESSIFCQLRSEDPDKAVTLVRSILAKKHQAVQGLRQQLSTCQEAVSFWQHQHTQWEEEGEALRQRLQKLTGERDVLAGQTQGLQGEVDSLNRDRGHLEKAREELQQQLEILEQEAWRLRRLNTELQLQGDLAQGEKVEQEEELQQAVRERDCLQETLKGLEARQSSSLSELISLKEALEKSHLEGELLNQERAEMARALAKAEQSIAELTGTERTLKAEVADLRDAAAKLSALNEALALDKVELNQQLLQLEQENQSVHGKVEASEKARSAVQMELDESEQRKEALLEEKACLEMQLQKSKDAEAELQGDLSREREEKIETQEKLNEAQRQLEVATTELEQLHQETTRQELLREKVIQEKEILVRDKAALEVHLKALERDQQDLSEQLLELSSAKELLETNLFEAQQQGSLMEITKEQLMMQIQTVTQAKEVIQGEVKCLQMELEAQRQQAEQEQDTAAKHRDQMEQESQAALQQQRATYEEELHLLQEERKMDQIKHRQELEATLEQMKREKVDLEETCEKKLRELQEESEAALAQRIKDHAQAESNRCQMQLEMEKERMSLLETLLKTQNELADAGHQLEQLRQDIKDQKLKEQENMEDLWAQLQEAQKTLMETRRKHHNDLAALEEEKSILLQQKENLEQQVQDLKAQLVTSEDSCRLAEHEAQKHLQEVQQCTHIQEEREQEKASLVQSLAEKEQRLLILQEADSLQQQELSSLRQEVKEAQEEQEQLSAQVLFLKEEMQKKEASLLDQEILLEKLGASKKTEQQLRASLWAQEAKAAQLQLQLCSVESQVGALILEQQNGHQAQAQLMGLYSILQQAFGSAIESRPELSGAGDASPLSWSPRTLEPGQENFLKNQIAVTNFTTEDVASLLHKLQRDLWQARHTRDDMKCQVEKLEQHLSQAEAERNQFSTEIQELQSLLSQNQKEKLKWEGEQSSLETELAVLQERVASLQNSLKRSEQQRTEAQNEQATLWAANEKLVSEVEHLQASVAVTKAQANAREALEAELRATHSALRMKNEEAESEREKAEALRKKLAQGKALQENVDLLTSTLSKREKELEALQGVIQELEKQRESQMVMQEHLSLNLKKRSQELESRQEQIQELEKQKAGLEHLPVALEQKKKQIRLQREQIKELEGKQETQGNILEHQALDLQKKTQTLESQREHIQDLENQLVTLEHLVLEVKKNHQEIESQKKKIEDLENQREMQRVTLTHLTLDLEEKSQEIQSQNKKIKELEKHNALLTVNLEERCQEMKSYRDQVEELKKQKDQLTWDLEKKNQELVLQEEKILVLEDQRMLQNRVLEEDFEQMKLALREKDQEIESQRQLMIEREEEEKGQAKAQRSSLEHMKLILRDKEKEVEYQRERIHMFQQYGEQREEQLQGLRRKIGEMTLLLTQKDQELETQQQHIRRVQERRESRERALQDQLERVQEALQTKVQDLEALQQKAQLQQESQEEQMSTLQAALEQARAALEEHEEAIEAHKEQNQKVQQQQTAAELQVQALEELLGDLRAEFQEQEKVLETLRQQCTEQGHEQETQAWHQHTEARQWEKTTAGQVQALKEALRVAQASLQKKDQELLQQAELSQRLEGSAAALQVALDSCQTQARHLEEALREREEEIREQQLQHQEMVQQLQLALAQRDGELRQLKEKGQQQEAALNQSDKGEEKEEKVLRQNLRHLQLTLSRKEFQLQETHRRHGQESYYESHSPEKMEDGEEEEVYGTGTMEKMSLREISPVSMEEAKLQEELERVKNVLRQTEAREIEWQEKAQALAFSLAESEASICNLREAALFLQAKTAEQNTEQLQLEEQLQLTRQALEDEQSHNRGSAWRTEPEIQGDEGTSQRKATGPEDDGSCTELEKTQQWQQRLKYLLHAMASLEHERNRLQRHNFQLRVTLEQVERERRKLKRNFNRMTHTTPPLPRETTALTPGQKEKGGQENSSELKHVAELQRQVTALQTQLALERQQKQDYISRCVQTSRELTDLHHNLSNSLWAVARAPEANILEAEARKLDKSLTQSLTSSAPSPPCCTPRSAQATSRS

Target ORF information:

RefSeq Version	XM_007474243.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007474243.2

ORF Insert Sequence:

ATGGAGGCAA GAGACCAAGG GCACAACATG AAGCCCCAGC CTCTGAATAT GGTGCTGGAG 
AAGCAGATCC TGGCACTGCA GCAGCAAATG GCCGAGAACC AGGCTGCTTC CTGGCACAAG 
CTGAAGATCT CCCAGGAGGC CCAACAGAGA CAAGCAGTAC TTGTGAGAAA GTTGCAAGCC 
AAGGTACTAC AGTACAGAAA CTGGTGTCAG GAACTAGAGA AGAGACTGGA GGACATGGGG 
GGACCTATGC CAAAGAGGTG GACAAGTGCT GAAGAGCCCA GACTGGAGCA GCTGCTGATC 
CGGCTGGAGG AAGAGCACCA GAGGTCTGAG GGTTTGGTGA AAGTGAACAC GCAGCTTCAG 
GAGCACCTGG AGGAAGCTGA TGTGGTGAAC AAGGCCCTTC AGGAGGATGT GGGGAAGTTA 
ACAGTGGACT GGAGCCAAGC TCAGGATGAG CTGGCAAGGA GACAGAGTGA CTGGCAAATG 
GAGAGGGAGT TCTTCAAGGG CTACCTGAAA GGGGAACATG GCCGCCTTCT CAGCCTGTGG 
CGGGAGGTGG TGACCTTCCG ACGCCATTTC CTGGAAATGA AGACAGCCAC TGACAGGGAT 
TTGGCTGAGC TAAAAGCTGA ACAAGTGAAG ATTTCTGGTT CCCTCCTGAC CAGCTGCCTG 
CACCTGAGCT TAGAATTACA GTCCCGGGAG GCTCAGGGGT CAGGGAGACT GGCTGGCAAT 
GAGCAGGTGC ATCTACAACT AGTAGCCAAA ACCCAGGAGC TGGAGAAGGA AATACACAAA 
AGAAGCCAAG AACTTATACT CCTGAAGGCT AGAGGGAACA TGGAGAAGAC AGAACTTCAG 
GATAGGGTGA CTGAACTTTC TGCTTTGCTG GCTCAGAGTA AAAAACAAAA TGAGGAGCAG 
GAGAAGATGT TGAAGGCTCT GAAGGACACC ATGGATATCC TGGAGACAAA TCGATCAGAA 
TTAATGGAAC ATGAGGCATC TCTCTCCAGA AATTCTCAGA AAGAGAAGTT GTCTTTGCAG 
CAAGTGATCA AGGACATAAC CCAGGTAGTG GTAGATGATG AAGACAGTGA AAATCAAAGT 
TTTGGTCTGG AGAGCTCCCT AGAGATGGAG TCTAGCATTT TCTGCCAGCT GCGTTCTGAA 
GATCCAGACA AGGCTGTCAC ACTGGTGCGC TCGATTCTGG CGAAGAAGCA CCAGGCAGTG 
CAGGGCCTGA GGCAGCAGCT TTCAACCTGT CAAGAGGCTG TGAGCTTCTG GCAGCATCAG 
CATACCCAGT GGGAGGAAGA GGGCGAGGCC CTGAGGCAGC GACTCCAGAA GCTCACTGGG 
GAACGGGACG TCCTGGCAGG ACAGACCCAG GGCCTTCAGG GGGAAGTCGA CTCTCTCAAC 
AGAGATCGAG GGCACCTGGA AAAGGCTAGG GAGGAGCTCC AGCAGCAGCT GGAGATTCTG 
GAACAAGAGG CCTGGCGCCT CCGCCGACTC AACACAGAGC TTCAGCTGCA AGGGGACTTG 
GCTCAGGGGG AGAAGGTGGA GCAGGAAGAG GAGCTGCAGC AAGCTGTTCG AGAGAGGGAT 
TGCCTTCAAG AGACTTTGAA GGGCCTGGAA GCCAGACAGT CTTCATCATT GAGTGAGCTG 
ATCTCCCTGA AGGAGGCACT AGAAAAGAGC CATCTTGAAG GGGAGCTGCT GAATCAAGAG 
AGAGCAGAGA TGGCAAGAGC TCTGGCTAAG GCAGAACAGT CAATTGCTGA ACTGACAGGA 
ACAGAGAGAA CTCTCAAGGC AGAAGTAGCT GATCTTCGGG ATGCAGCAGC CAAGCTGAGC 
GCACTGAATG AAGCCTTGGC CCTAGATAAA GTGGAGCTGA ACCAACAACT CTTACAGTTA 
GAGCAGGAAA ACCAGTCTGT CCATGGTAAA GTAGAGGCAT CAGAGAAAGC CCGAAGTGCT 
GTGCAGATGG AACTGGATGA ATCTGAACAG AGAAAGGAAG CCCTCTTGGA GGAAAAAGCT 
TGCCTTGAAA TGCAACTGCA AAAGTCTAAA GATGCTGAGG CAGAGCTTCA GGGGGATCTA 
AGCAGGGAGA GAGAAGAGAA GATCGAAACC CAGGAGAAAT TGAATGAGGC ACAACGACAG 
TTGGAGGTGG CCACTACAGA GCTGGAACAG CTGCACCAGG AGACAACTCG ACAAGAACTC 
TTGAGGGAGA AGGTGATCCA GGAGAAAGAG ATCTTGGTCC GAGACAAAGC TGCCCTGGAG 
GTGCACCTGA AGGCCTTGGA GCGGGACCAG CAGGATCTTT CTGAGCAACT ACTGGAACTC 
AGCTCAGCCA AGGAACTGTT AGAAACCAAC TTGTTTGAGG CTCAGCAGCA GGGCTCTCTG 
ATGGAGATTA CCAAGGAGCA GCTGATGATG CAGATTCAAA CAGTCACACA AGCCAAGGAG 
GTGATCCAAG GGGAGGTAAA GTGCCTTCAG ATGGAATTGG AAGCACAGCG ACAGCAGGCA 
GAACAGGAGC AGGACACAGC AGCCAAACAT CGAGACCAGA TGGAGCAAGA GAGTCAGGCA 
GCTCTACAGC AGCAGCGGGC CACCTATGAA GAAGAACTTC ACCTGCTGCA GGAGGAGAGG 
AAGATGGATC AAATCAAGCA CAGGCAAGAA TTAGAGGCCA CATTAGAACA AATGAAAAGA 
GAGAAAGTGG ACCTGGAGGA GACCTGTGAG AAGAAACTAA GGGAACTGCA GGAGGAGTCA 
GAAGCTGCCC TGGCTCAGAG GATAAAGGAT CATGCCCAAG CTGAGAGCAA CAGATGTCAG 
ATGCAGCTAG AGATGGAAAA GGAAAGGATG TCACTACTGG AGACACTCTT GAAGACCCAG 
AATGAACTAG CTGATGCTGG CCATCAATTG GAGCAGCTGA GGCAGGACAT AAAGGACCAG 
AAGTTAAAGG AACAGGAGAA TATGGAGGAC CTTTGGGCCC AGCTGCAAGA GGCTCAGAAG 
ACACTGATGG AGACAAGAAG AAAGCATCAC AATGACCTTG CTGCCCTTGA GGAAGAGAAA 
AGCATCCTGC TACAGCAAAA AGAGAACCTG GAACAACAGG TACAAGACTT GAAGGCCCAA 
CTAGTCACAA GTGAAGATTC ATGTAGGCTG GCAGAGCATG AGGCCCAGAA GCATCTACAA 
GAGGTCCAGC AGTGTACTCA TATCCAGGAG GAGAGGGAGC AGGAAAAGGC CAGCCTGGTT 
CAGTCACTAG CAGAGAAGGA ACAAAGACTC CTCATTTTAC AAGAGGCTGA TTCACTCCAA 
CAGCAAGAGC TGAGTTCCTT GCGTCAGGAA GTGAAGGAGG CCCAGGAAGA ACAGGAGCAG 
CTCAGTGCCC AGGTACTGTT TCTAAAGGAA GAGATGCAGA AAAAAGAAGC TTCTCTTTTG 
GATCAAGAAA TCCTTTTGGA GAAGTTGGGA GCATCAAAGA AAACAGAGCA GCAGCTGAGA 
GCCTCACTGT GGGCACAAGA GGCCAAGGCA GCCCAGCTGC AACTCCAGCT GTGCAGTGTA 
GAAAGCCAAG TAGGGGCTCT AATATTAGAG CAGCAGAACG GACATCAGGC ACAGGCACAG 
CTAATGGGTC TCTACTCCAT TCTGCAGCAA GCCTTTGGAA GTGCTATTGA GAGTCGGCCA 
GAGCTGAGTG GTGCGGGTGA TGCTTCTCCC CTTTCCTGGA GCCCCAGAAC TTTGGAGCCA 
GGACAAGAGA ATTTCCTTAA AAACCAGATT GCTGTAACGA ACTTTACTAC TGAGGATGTG 
GCATCTTTAC TTCACAAGCT CCAGCGAGAC CTCTGGCAGG CTCGTCATAC TCGGGATGAT 
ATGAAATGTC AGGTTGAGAA GCTGGAGCAA CATCTGTCCC AAGCTGAGGC TGAGCGGAAC 
CAGTTTTCTA CGGAAATTCA AGAGCTTCAA AGTTTGCTCT CCCAGAATCA AAAAGAGAAA 
TTGAAATGGG AGGGAGAGCA GAGCTCTTTG GAGACAGAGT TGGCTGTACT TCAGGAAAGA 
GTGGCTTCCC TGCAGAACAG TCTGAAGAGA TCTGAGCAGC AAAGGACAGA AGCCCAGAAT 
GAGCAGGCAA CATTGTGGGC AGCCAATGAA AAATTGGTAT CAGAGGTGGA ACACCTTCAA 
GCATCAGTCG CAGTCACCAA GGCACAAGCT AATGCCAGGG AGGCCCTGGA AGCAGAACTG 
AGAGCAACAC ACTCAGCCCT GAGAATGAAA AATGAAGAAG CAGAGAGTGA GCGAGAGAAA 
GCAGAGGCCC TAAGGAAGAA GCTGGCCCAA GGGAAGGCCC TGCAGGAAAA TGTTGATCTC 
TTGACCAGTA CCCTTTCTAA GAGAGAGAAG GAATTAGAAG CCCTGCAGGG AGTCATTCAA 
GAACTGGAGA AACAGAGAGA ATCACAAATG GTGATGCAAG AACATCTGTC TCTGAACCTA 
AAGAAGAGAA GTCAAGAGCT GGAATCCCGG CAAGAACAGA TCCAAGAGCT GGAGAAACAG 
AAAGCTGGAC TAGAACATCT GCCTGTGGCC CTGGAACAGA AAAAGAAACA AATACGGTTA 
CAGAGAGAGC AGATTAAAGA GCTTGAGGGA AAGCAAGAAA CTCAGGGAAA CATCCTGGAA 
CATCAAGCTC TGGATTTACA GAAGAAAACA CAAACATTAG AGTCCCAGAG AGAACATATT 
CAAGATCTGG AAAACCAGTT GGTCACTCTG GAACATCTGG TTTTAGAAGT GAAAAAGAAT 
CATCAAGAAA TAGAGTCTCA GAAAAAAAAG ATTGAGGACT TAGAGAACCA GAGAGAAATG 
CAGAGGGTCA CTCTGACCCA TCTTACTTTG GACTTGGAGG AGAAAAGTCA GGAAATACAA 
TCTCAGAACA AAAAGATTAA AGAGTTAGAA AAGCACAATG CTCTCCTGAC AGTAAATCTG 
GAGGAGAGAT GCCAAGAGAT GAAGTCCTAC AGAGACCAGG TGGAAGAGTT AAAGAAGCAA 
AAGGATCAGC TTACTTGGGA CCTAGAGAAA AAAAACCAGG AGTTGGTCCT ACAAGAAGAG 
AAAATTCTGG TCCTGGAAGA TCAGAGAATG TTGCAGAACA GAGTATTAGA GGAAGACTTT 
GAACAGATGA AATTGGCCTT GAGGGAAAAA GATCAGGAAA TTGAGTCTCA AAGACAGCTC 
ATGATAGAAC GGGAAGAGGA AGAAAAGGGA CAAGCCAAGG CCCAACGCAG CAGCCTGGAG 
CATATGAAGT TGATCCTGAG GGACAAGGAG AAGGAGGTCG AGTACCAGCG AGAGAGGATT 
CACATGTTCC AACAGTATGG AGAACAGCGG GAAGAGCAAC TACAGGGCCT GCGGAGAAAG 
ATTGGGGAGA TGACGCTGCT CTTAACTCAG AAAGATCAAG AGTTGGAGAC CCAACAACAG 
CATATCAGGA GAGTCCAGGA GAGACGGGAG TCCAGGGAAA GGGCCCTGCA GGACCAACTG 
GAGAGGGTGC AGGAGGCCTT GCAGACCAAA GTGCAAGACC TGGAAGCCCT CCAGCAGAAG 
GCACAATTAC AGCAGGAAAG CCAGGAAGAA CAGATGAGCA CTTTGCAAGC AGCCCTGGAA 
CAGGCCAGGG CAGCTCTGGA GGAGCATGAG GAGGCAATTG AGGCTCACAA GGAACAGAAC 
CAGAAAGTAC AGCAACAACA AACGGCTGCA GAGCTGCAGG TTCAAGCTTT GGAAGAGCTG 
CTAGGAGATC TCAGGGCTGA GTTTCAGGAG CAAGAGAAGG TTCTGGAGAC CCTGCGACAG 
CAGTGTACAG AGCAAGGACA TGAGCAGGAG ACACAGGCCT GGCACCAGCA CACAGAAGCC 
AGGCAGTGGG AAAAAACTAC AGCAGGCCAG GTCCAGGCCC TCAAGGAAGC CCTCAGAGTG 
GCCCAGGCCT CCCTTCAGAA GAAAGACCAG GAGCTCCTCC AGCAGGCAGA ACTAAGCCAG 
CGCTTGGAAG GCAGTGCAGC CGCTTTGCAG GTGGCCTTGG ACTCATGTCA GACTCAGGCC 
CGGCACCTGG AGGAGGCCCT GAGGGAGCGA GAGGAAGAGA TCAGGGAGCA GCAGCTACAG 
CACCAAGAAA TGGTACAGCA ACTGCAGCTG GCCCTGGCCC AGAGGGATGG GGAGCTGAGG 
CAACTCAAGG AAAAGGGGCA GCAGCAAGAG GCAGCCCTGA ACCAATCAGA CAAAGGTGAG 
GAAAAAGAAG AGAAGGTCCT GAGACAAAAC CTCAGGCATC TCCAGCTGAC CTTGTCCAGA 
AAGGAGTTCC AGCTGCAGGA GACCCACCGG AGGCATGGAC AGGAGTCTTA CTATGAAAGC 
CACAGCCCAG AGAAAATGGA GGATGGGGAG GAGGAGGAGG TCTATGGGAC AGGGACGATG 
GAGAAAATGT CCCTCCGTGA GATATCGCCT GTGTCCATGG AAGAGGCCAA GCTGCAAGAG 
GAGCTTGAGA GAGTGAAGAA TGTCCTGAGG CAAACAGAGG CTCGGGAAAT TGAGTGGCAA 
GAGAAGGCCC AAGCCTTGGC ATTCTCCCTA GCTGAGAGTG AAGCCAGCAT CTGTAACCTG 
CGTGAGGCTG CCCTATTCTT ACAGGCAAAG ACAGCAGAGC AAAACACAGA ACAGCTACAA 
CTTGAGGAGC AGTTGCAGCT CACAAGACAA GCTCTAGAGG ATGAGCAATC CCACAACCGG 
GGCTCAGCTT GGAGGACAGA GCCAGAGATC CAGGGAGATG AGGGTACTTC CCAAAGAAAG 
GCCACAGGAC CAGAGGATGA TGGATCTTGT ACAGAACTTG AGAAGACACA GCAATGGCAG 
CAAAGGCTAA AGTATCTGCT ACATGCTATG GCAAGTCTAG AGCATGAGAG GAACCGGCTT 
CAGCGACACA ACTTCCAATT GAGGGTCACC TTAGAGCAGG TAGAACGGGA GCGGAGGAAA 
CTGAAGAGGA ATTTTAACCG TATGACCCAT ACTACACCTC CCCTGCCTAG GGAGACCACA 
GCCCTCACAC CAGGCCAGAA GGAGAAAGGG GGACAGGAGA ATTCCTCAGA ATTGAAACAT 
GTGGCTGAGT TACAGAGGCA GGTGACAGCC CTGCAGACAC AGCTGGCACT GGAGCGGCAG 
CAGAAACAGG ACTACATCTC CCGCTGTGTG CAGACAAGCC GAGAGCTAAC AGATCTGCAC 
CACAACCTTT CCAACTCACT GTGGGCTGTA GCTCGGGCCC CTGAAGCCAA CATCCTGGAG 
GCAGAGGCCC GAAAGCTAGA TAAGTCACTG ACCCAGAGCC TGACATCTTC CGCACCATCC 
CCACCCTGCT GCACTCCCAG GTCAGCCCAG GCTACCTCCC GTAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CEP250 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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