Anti-VHH Antibodies

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Anti-VHH Antibodies

VHH nanobody is a promising tool in therapeutics and immunodiagnostics applications, such as developing recombinant antibody drugs and CAR-T therapy. Due to the unique structure, VHHs overcome many of the limitations of traditional antibodies, such as long development times, low stability, and strict storage requirements, making them an increasingly important tool in biomedicine and clinical diagnostics. GenScript has developed a series of rabbit monoclonal (MonoRab™) anti-VHH antibodies to facilitate your research of VHHs.

: Exceptional Specificity
No cross-reactivity with mouse, rat, rabbit, goat, and human immunoglobulins

: Versatile Applicability
Ability to recognize camelid and humanized VHHs

: High Quality and Consistency
Monoclonal antibody manufacturing process consistently ensures high standards of quality, performance, and reliability

Applications

: High-throughput screening to identify VHH clones

: Sorting CAR-T cells for further expansion or functional testing

: Purification of VHHs

: Detection and Quantification of VHHs for WB, ELISA, IF, etc.

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Overview Selection Guide All Products Application Data Resources

Selection Guide

Product Type	Species Specificity	Unconjugated	Conjugated
Product Type	Species Specificity	Unconjugated	HRP	Biotin	iFluor 488	iFluor 555	iFluor 647	PE	FITC
Anti-Camelid VHH, mAb	Llama, Camel, Alpaca	A01860	A01861	A01995	A01862	A01863	A01994	A02227	A02226
Anti-Humanized VHH, mAb	Humanized, Llama, Camel, Alpaca	A02165	A02167	A02166	A02168	A02169	A02170	A02171	A02172
Anti-Camelid VHH, mAb Cocktail	Llama, Camel, Alpaca	A02014	A02016	A02015	A02021	A02020	A02019	A02018	A02017

Performance

MonoRab™ Rabbit Anti-Camelid VHH Antibody (GenScript Cat. No A01860)

Binding compatibility of Anti-Camelid VHH Antibody (A01860) with 20 different VHHs

Cross reactivity of Anti-Camelid VHH Antibody (A01860) with lgG of different species

ELISA binding of Anti-Camelid VHH Antibody (A01860) with different subclasses camelid lgG

ELISA of Anti-Camelid VHH Antibody (A01860) with denatured or native camelid IgG
MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb (GenScript Cat. No A02165 )

Binding compatibility comparison of MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb (Cat. No A02165 ), MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb (Cat. No A01860 ), and MonoRab™ Rabbit Anti-Camelid VHH Cocktail (Cat. No A02014 ) with humanized VHHs. The performance of A02165 is the best. It is ideal to be used for humanized VHH antibody detection.

MonoRab™ Rabbit Anti-Camelid VHH Cocktail Antibody (GenScript Cat.A02014)

Binding compatibility comparison of Anti-Camelid VHH Antibody (A01860) and Anti-Camelid VHH Antibody (A02014) with 17 different VHHs

The Affinity of Anti-Camelid VHH Antibody (A02014)

Cross reactivity of A02014 with lgG of different species

ELISA binding of A02014 with different subclasses camelid lgG

Product Type	Species Specificity	Unconjugated	Conjugated
Product Type	Species Specificity	Unconjugated	HRP	Biotin	iFluor 488	iFluor 555	iFluor 647	PE	FITC
Anti-VHH, mAb	Llama, Camel	A01860	A01861	A01995	A01862	A01863	A01994	-	-
Anti-VHH, mAb Cocktail	Llama, Camel, Alpaca	A02014	A02016	A02015	A02021	A02020	A02019	A02018	A02017

Application

FACS

Figure 1. Identification of CAR-T Cells by Flow Cytometry Using Different Concentrations of MonoRab™ Rabbit Anti-Camelid VHH Cocktail [PE] (GenScript，A02018)

As CAR-T therapies continue to advance, the use of VHH in CAR design has become increasingly common. This antibody demonstrates excellent binding activity to Jurkat-CAR cells based on VHH nanobodies, and is recommended for use in CAR-T cell validation and identification.
MACS

Figure 2. Application of MonoRab™ Rabbit Anti-Camelid VHH Cocktail [Biotin] (GenScript，A02015) in Magnetic Separation of CAR-T Cells.

In this experiment, primary T cells were used as CAR-negative controls. A mixture containing 9.43% CAR-positive T cells and primary T cells served as the positive sample for separation. Magnetic separation of CAR-T cells was performed using the biotin-labeled MonoRab™ Rabbit Anti-Camelid VHH Cocktail [Biotin] (GenScript, A02015) together with anti-biotin magnetic beads. The separated cells were analyzed by flow cytometry to evaluate the separation efficiency. As shown in the figure, the proportion of CAR-positive cells increased from 9.43% in the pre-mixed sample to 88.9% after magnetic separation, demonstrating that this antibody is well-suited for CAR-T cell magnetic separation.
Sandwich ELISA Application

Standard curve of VHH Sandwich ELISA. The VHH Sandwich ELISA assay is developed by using MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb (GenScript, A01860) and MonoRab™ Rabbit Anti-Camelid VHH Cocktail [HRP] (GenScript, A02016) as the capture and detection antibodies, respectively.

In this ELISA assay, the detection antibody was labeled with horseradish peroxidase (HRP), if you needs an unconjugated antibody you can choose MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014). GenScript can provide customized conjugation services for this product per the customer's request.

The sensitivity of detecting VHH is up to 39 pg/ml.