GenScript's Plasmid DNA Preparation Service is accommodating to both small research labs as well as large-scale manufacturing biotech and pharmaceutical companies, providing you with high quality plasmids with 100% full insert sequence accuracy. Choose from a variety of our packages to help you achieve highly efficient cell transfection, and to support your DNA vaccine development, antibody production, and other preclinical research projects. Request a quote now for New plasmids to be synthesized, or Re-prep of previously synthesized plasmid.
With close to 20 years of CRO experience, GenScript truly understands your needs. From transformation to extraction, we have developed a robust protocol with years of process optimization to provide you top-notch quality plasmid preps with fully customizable services.
Research Grade
Industrial Grade
Pre-clinical Grade
Plasmid prep shouldn't be a time and energy-consuming job in your lab! Highly economical, GenScript's research grade plasmid DNA can help you with standard preps for cloning, mutagenesis and transformation. From microgram- to gram-scale with reliable quality and fast turnaround time to accommodate tight deadlines.
Are uncontrolled or unexplained failures in your plasmid prep causing reproducibility issues and delays in your experiments? GenScript's Industrial Grade Plasmid DNA can help your experiments achieve highly efficient cell transfection, helping to improve experimental outcomes in research areas such as protein expression, antibody production, and other research projects.
GenScript offers preclinical grade plasmids with the most comprehensive and stringent quality specifications and analysis for your preclinical project needs. Ideal for preclinical in vivo animal studies, from mice to non-human primates, in gene therapy, cell therapy, and vaccine research. Available in quantities of 10 mg and up.
| Specs | Research Grade | Industrial Grade | HT Grade | Pre-clinical Grade |
|---|---|---|---|---|
| Price | From $39 | From $99 | From $29 | From $1199 |
| Volume | 100 µg-2 g | 100 µg-2 g | 10 µg-30 µg | 10 mg-2 g |
| Turnaround Time | From 2BD (bundled)
From 8BD (stand alone) |
From 2BD (bundled)
From 8BD (stand alone) |
From 2BD (bundled)
From 9BD (stand alone) |
From 2BD (bundled)
From 9BD (stand alone) |
| Homogeneity | Predominantly supercoiled | ≥90±10% supercoiled | ≥90±10% supercoiled | ≥90% supercoiled |
| Endotoxin | - | Quantitative LAL assay <0.01 EU/µg |
Quantitative LAL assay <0.1 EU/µg |
Quantitative LAL assay ≤0.01 EU/µg or ≤0.005 EU/µg |
| Residual RNA | Non-detectable by gel electrophoresis at 200ng | |||
| Residual E. coli DNA | Non-detectable by gel electrophoresis | Quantitative PCR ≤ 5% |
||
| Residual Host Protein | - | HCP ELISA ≤1% | ||
| Bioburden Testing | - | No growth on agar plate after 48 hours | ||
| Animal Free Production* | Available with additional charge | |||
| Enzyme Free Production | - | Available with additional charge | ||
| Advanced Endotoxin Removal <0.005 EU/µg | - | Available with additional charge | ||
| Mycoplasma Contamination | - | Available with additional charge | ||
| Material Archiving | - | Available with additional charge | ||
| Appearance | Clear, colorless, no visible particulates | |||
| A260:280 | 1.8 - 2.0 | |||
| pH | - | 8.0 ± 0.5 (in TE buffer) 7.4 ± 0.5 (in PBS buffer) (5-7) ± 0.5 (in ddH2O buffer) |
||
| Restriction Analysis | Conforming to reference pattern | |||
Additional QC available upon request
Animal Free Statement
Plasmid Prep QC
*: For research, industrial and HT grade plasmid preps: Since densitometry is semi quantitative, the genomic DNA is considered nonvisible if the comparative intensity of genomic DNA < 15% of plasmid band. Quantitative genomic DNA measurement by qPCR is available with additional charges.
**: Sequence verification of the full gene insert only applies when bundled with gene synthesis (an insert to be cloned into a plasmid).
***: Percentage of supercoiled plasmid may vary when analyzing with different methods or can be altered due to shipping conditions.
Gene Therapy Tidbits
Learn how improved ITR integrity can impact AAV plasmid expression and accelerate your gene therapy pipeline!
GLP Plasmid Prep Services
As a leading biotech company and the largest gene synthesis supplier in the USA, GenScript has
accumulated a wealth of experience in GLP-level plasmid preparation.
We are happy to offer GLP
plasmid prep services to provide you with ultra transparent documentation system and communication.
Strict
Operating Procedurces
Comprehensive
Final Report
Data Archive
Management
Testimonials
We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!
”Dr. Melissa A. Melan,
University of Pittsburgh Medical Center, USA
