Efficient and gentle cell isolation, based on surface markers is essential for cell and gene research. Separating the desire cell from the rest of the sample minimizes interference and is key to identifying, studying, and analyzing specific cell populations for downstream application.
GenScript has renowned expertise in developing magnetic beads products for bio separation of various types of biomolecules. Our new CytoSinctTM magnetic beads are powered by immunomagnetic cell separation technology to isolate desired cell populations, by combing combining the specificity and selectivity of antibody based purification with gentleness of nanoparticles.
The CytoSinctTM cell separation manual kit is composed of antigen-specific paramagnetic Nanobeads, column and magnetic separator. The CytoSinctTM beads are nanometer-sized, coated with biodegradable matrix, nontoxic, easy to use, and enable highly efficient cell isolation. The CytoSinctColumnTM amplifies the magnetic field and enables efficient separation with minimum labeling. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis, and translational research.
Armed with the flexibility and specificity of the CytoSinctTM cell separation kit, you can use for variety of starting materials including PBMC or Leukapheresis, for isolation or depletion different cell type including T cell, NK cell, CD34 cell, TCR alpha beta etc.
For every experimental application, the CytoSinctTM cell separation technology can provide you with the separation results you require. GenScript’s CytoSinctTM Beads and columns are also compatible with column based cell separation platforms made by other manufacturers.
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Schematic diagram showing the structure and size of CytoSinct™ Nanobeads.
CytoSinct™ Cell Isolation kit including Nanobeads, Columns, and Magnetic Separator.
GenScript's CytoSinct™ Nanobeads purify cells using a column-based separation method.
*These beads are compatible with magnetic columns for cell isolation from GenScript or another vendor.
Efficient and gentle cell isolation based on surface markers is essential for researching specific cell populations, and for developing lifesaving cell and gene therapies. Purity, recovery and viability are among the most important characters of cells after isolation. CytoSinctTM Nanobeads are nanometer-sized beads that isolate your target cells gently and efficiently, making your post-isolation cells suitable for a variety of down-stream applications. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis, and translational research.
• High Purity, Efficient Recovery, High Viability, High Specificity
Human frozen PBMC samples were incubated with GenScript’s CytoSinctTM CD4 Nanobeads, isolated using column method, and analyzed using flow cytometry.
Figure 1: Figure shows representative pre- and post-isolation data of Purity, recovery, and viability of CD4+ T-cells.
Figure 2: Figure shows comparison of pre- and post-isolation data of different PBMC samples as starting material, using CytoSinctTM CD4 nano beads and competitor reagent.
For this expt Human PBMC samples were incubated with GenScript CytoSinctTM CD4 Nano beads and competitor’s reagents. Cells were then isolated using column method and analyzed using flow cytometry. Pre- and post-isolation samples were stained with the cell viability dye 7-AAD and with cell surface markers CD4+ CD45+, cells are gated from scatter/ singlets/ Live.
Purity, Recovery and Viability for CytoSinctTM CD4 nano beads are comparable or better than competitor.
Figure 3:Figure Shows comparison of pre and post isolation of T cell from Leukapheresis using GenScript CytoSinctTM CD4 Nanobeads and competitor products.
Performance of GenScript’s beads are robust and efficient for any start samples.
Efficient and gentle cell isolation based on surface markers is essential for researching specific cell populations, and for developing lifesaving cell and gene therapies. Purity, recovery and viability are among the most important characters of cells after isolation. CytoSinctTM Nanobeads are nanometer-sized beads that isolate your target cells gently and efficiently, making your post-isolation cells suitable for a variety of down-stream applications. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis, and translational research.
• High Purity, Efficient Recovery, High Viability, High Specificity
Human frozen PBMC samples were incubated with GenScript’s CytoSinctTM CD4. Nanobeads, isolated using column method, and analyzed using flow cytometry.
Figure 1: Figure shows representative pre- and post-isolation data of Purity, recovery, and viability of CD4+ T-cells.
Figure 2: Figure shows comparison of pre- and post-isolation data of different PBMC samples as starting material, using CytoSinctTM CD4 nano beads and competitor reagent.
For this expt Human PBMC. samples were incubated with GenScript CytoSinctTM CD4 Nano beads and competitor’s reagents. Cells were then isolated using column method and analyzed using flow cytometry. Pre- and post-isolation samples were stained with the cell viability dye 7-AAD and with cell surface markers CD4+ CD45+, cells are gated from scatter/ singlets/ Live.
Purity, Recovery and Viability for CytoSinctTM CD4 nano beads are comparable or better than competitor.
Figure 3: Figure Shows comparison of pre and post Co-isolation data of CD4 + CD8 T cell from Leukapheresis using CytoSinctTM CD4 + CytoSinctTM CD8 Nano beads and competitor reagent .
Performance of GenScript’s beads are robust and efficient for any start samples.
Efficient and gentle cell isolation based on surface markers is essential for researching specific cell populations, and for developing lifesaving cell and gene therapies. Purity, recovery and viability are among the most important characters of cells after isolation. CytoSinctTM Nanobeads are nanometer-sized beads that isolate your target cells gently and efficiently, making your post-isolation cells suitable for a variety of down-stream applications. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis, and translational research.
• High Purity, Efficient Recovery, High Viability, High Specificity
Human frozen PBMC samples were incubated with GenScript’s CytoSinctTM CD8 Nanobeads, isolated using column method, and analyzed using flow cytometry.
Figure 1:Figure shows representative pre- and post-isolation data of Purity, recovery, and viability of CD8+ T-cells.
Figure 2: Figure shows comparison of pre- and post-isolation data of different PBMC samples as starting material, using CytoSinctTM CD8 nano beads and competitor reagent.
In this expt Human PBMC samples were incubated with GenScript CytoSinctTM CD8 Nano beads and competitor’s reagents. Cells were then isolated using column method and analyzed using flow cytometry. Pre- and post-isolation samples were stained with the cell viability dye 7-AAD and with cell surface markers CD8+ CD45+, cells are gated from scatter/ singlets/ Live.
Purity, Recovery and Viability for CytoSinctTM CD8 nano beads are comparable or better than competitor.
Figure 3: Figure Shows comparison of pre and post Co-isolation data of CD4 + CD8 T cell from Leukapheresis using CytoSinctTM CD4 + CytoSinctTM CD8 Nano beads and competitor reagent .
Performance of GenScript’s beads are robust and efficient for any start samples.
Product List
| Catalog Number | Product Name | Package Size | Capability |
|---|---|---|---|
| L00863-1 | CytoSinct™ CD4 Nanobeads, human (RUO) | 1 mL | Up to 1X109 total MNCs |
| L00863-0.5 | CytoSinct™ CD4 Nanobeads, human (RUO) | 500 uL | Up to 5X108 total MNCs |
| L00864-1 | CytoSinct™ CD8 Nanobeads, human (RUO) | 1 mL | Up to 1X109 total MNCs |
| L00864-0.5 | CytoSinct™ CD8 Nanobeads, human (RUO) | 500 uL | Up to 5X108 total MNCs |
| L00896-1 | CytoSinct™ CD3 Nanobeads, human (RUO) | 1 mL | Up to 1X109 total MNCs |
| L00896-0.5 | CytoSinct™ CD3 Nanobeads, human (RUO) | 500 uL | Up to 5X108 total MNCs |
| L00903-1 | CytoSinct™ CD56 Nanobeads, human | 1 mL | Up to 1X109 total MNCs |
| L00903-0.5 | CytoSinct™ CD56 Nanobeads, human | 500 μl | Up to 5×108 total MNCs |
| D00006 | CytoSinct™ gStand | 1 pc | Compatible with all CytoSinct™ Magnets |
| D00007 | CytoSinct™ gM columns | 25 pc | Up to 107 magnetically labeled cells from up to 2×108 total cells (each column) |
| D00008 | CytoSinct™ gL columns | 25 pc | Up to 108 magnetically labeled cells from up to 2×109 total cells (each column) |
| D00009 | CytoSinct™ M1 magnet | 1 pc | Holds 1 CytoSinct™ gM column |
| D00010 | CytoSinct™ M8 magnet | 1 pc | Holds 8 CytoSinct™ gM columns |
| D00011 | CytoSinct™ L1 magnet | 1 pc | Holds 1 CytoSinct™ gL column |
| D00012 | CytoSinct™ L4 magnet | 1 pc | Holds 4 CytoSinct™ gL columns |
