GenScript's Benz-Neburase™ is a highly effective, nonspecific, genetically engineered endonuclease capable of digesting all forms of DNA and RNA into 3-5 base nucleotides. The nucleic acid forms include double stranded, single stranded, linearized, and circular forms.
The Benz-Neburase™ offerings provide cost-effective solutions for removing contaminating nucleic acids. This endonuclease is commonly used in biopharmaceutical production such as vaccine, viral vector, gene and cell therapy manufacturing facilities.
Powerful
Highly effective, nonspecific endonuclease capable of digesting all forms of DNA and RNA.
Fast
One unit of Benz-Neburase is capable of digesting 37μg of DNA in less than 30 minutes.
Pure
SDS-PAGE analysis of Benz-Neburase showed the enzyme at >95% purity.
- Benz-Neburase™ GMP, tag-free
- Benz-Neburase™, tag-free
- Benz-Neburase™ GMP, 6x histidine tag
- Benz-Neburase™, 6x histidine tag
- Benz-Neburase™ Nuclease ELISA Kit
Benz-Neburase™ GMP, tag-free
Benz-Neburase™, tag-free
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03695-500 |
Benz-Neburase™, tag-free - 500 kU | - | |
Z03695-100 |
Benz-Neburase™, tag-free - 100 kU | - | |
Z03695-10 |
Benz-Neburase™, tag-free - 10 kU |
$49.00 |
Benz-Neburase™ GMP, 6x histidine tag
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03627-500 |
Benz-Neburase™ GMP, His-tag - 500 kU |
$900.00 |
|
Z03627-100 |
Benz-Neburase™ GMP, His-tag - 100 kU |
$225.00 |
|
Z03627-10 |
Benz-Neburase™ GMP, His-tag - 10 kU |
$58.50 |
Benz-Neburase™, 6x histidine tag
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03626-500 |
Benz-Neburase™, His - 500 kU |
$600.00 |
|
Z03626-100 |
Benz-Neburase™, His - 100 kU |
$150.00 |
|
Z03626-10 |
Benz-Neburase™, His - 10 kU |
$39.00 |
Benz-Neburase™ Nuclease ELISA Kit
Impact of reaction conditions on the performance of Benz-Neburase™
| Condition | Optimal* | Effective** |
|---|---|---|
| Mg2+ | 1 - 2 mM | 1 - 10 mM |
| pH | 8.0 - 9.2 | 5.0 - 11.0 |
| Temperature | 37 - 45 ℃ | 22 - 55 ℃ |
| Salt ions (Na+, K+, etc.) | 0 - 20 mM | 0 - 300 mM |
| PO43- | 0 - 10 mM | 0 - 40 mM |
| Urea | 4 M | 0-6 M |
| SDS | SDS inactivates Benz-Neburase in 10 minutes at any concentration. | |
* Optimal is defined as the condition under which Benz-Neburase retains > 90% of its activity.
** Effective is defined as the condition under which Benz-Neburase retains > 15% of its activity.
Figure 5: The test results show that Benz-Neburase can remove DNA and plasmid residue in virus production process. Fluorescence Activated Cell Sorting (FACS) was used to determine the functional titer of lentiviral particles. The analysis data indicate that the use of Benz-Neburase has minimal impact on the viral production recovery. The data further indicates that the GenScript Benz-Neburase outperforms the competitor product.
Application:
- Expression system: HEK293
- Virus type: LV
- Process flow: adding nuclease to virus supernatant
- Purpose: Sample plasmid residue and host DNA residue
- Dosage: 20 units/mL
- Temperature: 37°C
- Reaction time: 1 h
Figure 6: The test results show that Benz-Neburase can also remove DNA and plasmid residue in AAV virus production process more effectively than the competitor product. Viral genome (Vg) recovery was used to quantify the adeno associated viral production. The analysis data indicate that the use of Benz-Neburase has minimal impact on the viral production. The data further indicates that the GenScript Benz-Neburase outperforms the competitor product, while the Benz-Neburase GMP has comparative performance.
Application:
- Expression system: HEK293
- Virus type: AAV
- Process flow: adding nuclease to virus supernatant
- Purpose: Remove sample plasmid residue and host DNA residue
- Dosage: 50 units/mL
- Temperature: 37°C
- Reaction time: 1 h
