trim28 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	trim28
Entrez Gene ID	100489687
Full Name	tripartite motif containing 28
Synonyms	kap1,rnf96,tf1b,tif1b
General protein information	Preferred Names tripartite motif containing 28 Names transcription intermediary factor 1-beta
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004916207.3	XP_004916264.1	transcription intermediary factor 1-beta isoform X1
XM_012967404.2	XP_012822858.1	transcription intermediary factor 1-beta isoform X4
XM_018095471.1	XP_017950960.1	transcription intermediary factor 1-beta isoform X3
XM_002937602.4	XP_002937648.1	transcription intermediary factor 1-beta isoform X2
XM_002937602.5	XP_002937648.1	transcription intermediary factor 1-beta isoform X1
XM_031905703.1	XP_031761563.1	transcription intermediary factor 1-beta isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRIM28	XP_533567.4
Xenopus tropicalis (tropical clawed frog)	LOC100489687	XP_004916264.1
Macaca mulatta (Rhesus monkey)	TRIM28	XP_001098702.2
Mus musculus (house mouse)	Trim28	NP_035718.2
Rattus norvegicus (Norway rat)	Trim28	NP_446368.1
Bos taurus (cattle)	TRIM28	NP_001193738.1
Homo sapiens (human)	TRIM28	NP_005753.1

Gene Ontology

The following trim28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trim28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa13278	XM_004916207.3 Latest version!	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa13279	XM_002937602.5 Latest version!	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa34567	XM_012967404.2 Latest version!	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa45438	XM_018095471.1 Latest version!	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa62118	XM_031905703.1 Latest version!	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OXa13279	XM_002937602.4	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trim28 ( XM_004916207.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916264.1 Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa13278
Clone ID Related Accession (Same CDS sequence)	XM_004916207.3
Accession Version	XM_004916207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription intermediary factor 1-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916207.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTAG CTGCAACGCG TGACAAGCGG AGTGTCCTGA GCACCGACAA TGGCAACGGA 
GGCAGCGGGG AGCCCGACGG GCAGGAGCTA CTGGAGAGCT GCGGGGTGTG CAAGAAGCAG 
TTACGGGCGG ACACGGAGCC CCAGCTCTTA CCGTGCCTGC ACTCCGTGTG CCGGGCCTGC 
CTTCCGCCAG AGACCCCTGC GCCTGAGGTG GCCACTAACA GCGGAGACTT GGCTTCAGCT 
GTAGTTAATT GCCCCATATG CAAGCACCAG TGCTTCCAGA AGGATATTGT TGAGAACTAC 
TTTCTAAAAG TGGAACCCAC TTCCGAACCC GTAACTGAAA CAATTCAGAG ATGTACCAGC 
TGTGATGACA ATACTGTAGC AAATAGTTAC TGCGTCGAGT GCACAGAGTG GCTATGTGAG 
ACCTGTGTGG AGGCGCATCA GCGAGTGAAA TACACAAAGG ATCACACCAT CAAAGTTAAA 
AGTTCTTCCA AAAAGGAAAC AGAGCGAGCA GTTTACTGTC CTTTGCATAA AAATGAACCT 
CTCATGCTTT ATTGTGAATC CTGTGATACC TTAACTTGCC GGGACTGCCA ACTACAGGGA 
CACAAAGATC ACCAGTACCA GTTTTTAGAG GATGCTGTTA AAAATCAAAG GAAGGTACTT 
TCTGCTCTGG TCAAGCGGCT TGGAGATAAG CATACGGCTT TACAGAAATC TACTAAAGAT 
GTTCGAACTT CGATTCGACA GGTGAGTGAT TTACAGAAGA GATTGCAAGT GGATGTAAAG 
ATGGCAATCT TGCACATAAT GAAGGAACTT AATAAACGTG GAAAACTACT AGTGAATGAT 
ATGCAGAAAG TAACAGAAGG TCATCAGGAT AAACTGGAAA AGCAGCACTG GGCTATGAAC 
AAATTGCAAA AGCATCAAGA GCACATCCTT AGGTTTGCCA CGTGGGCACT GGAAAGTGAC 
AACAACATAG CCTTATTGCT TTCAAAGAAA CTGATACATT TTCAGTTGCA TCGTGCTCTA 
AAAGTTATTG TAGATCCAGT TGAGCCTTTA GGTGATTTAA AGTTCCAGTG GGACTCTGAT 
ACCTGGACAA AGCATGCAGA GTTTTTTGGC AAAATTGTAT GCGACAAAAC AGGTGTTCCT 
CAGTCATCTT CTGGATCGTT GGCAGCCCAG TCTATAAATT CTGCGTCATC TCAGGTGCTG 
GCTTCTCTTA CTAACTTTAA TCCAATGGTA CAGAATCGAC CAAGGCTTGG TGCTTCCCAG 
ATTCAAGCAT CTCCAGCCAG AGCTGGTACA GCTGGTAACT TCATCCAGCC CATGCAGATG 
ATGGATGACT TTGATGGGTC TTCTGCAGAG GGAGAATCTT CTGGATTTGA TACTTTCCCT 
GGCCAGAAAA GAGGGCGCAG TTCTGAAGGT GATGTAAATG AGCTTTTAAA AAAAGTTCCA 
CGTGTCAGTT TAGAGCGCTT GGATGTAGAC CTTAACCCCG ACACTCAACC ACCAGTCTTC 
AAAGTTTTCC CCGGAAACAC AAATGAAGAT TATAATTTAA TTGTAATTGA GAGAGGGAAT 
CTTCCAGGGG AAGCTGTTGT GAAGGAAGAA GCCATGGAAA CAGGCATCCC TATCCCAACA 
TCTGACCGCT CTGAAAAATC CTTAGTTATT CGACCAGATG GCTCGACAGA AACTGTGCCA 
CCTGTAATCC GCCAGCCTAC TGCAGCTGGT AATGCCACTT CCTCTGATTT CCAAGGAGCA 
AGCAGCGGGG GATTTTCTGA TGGGCTCAGT GGTGCCCGTT GTAAAGTGTG CCAGAAGGAA 
GGAGCCCTAC TGACATGTAG CCAATGTAGA AGCCTTTTCC ACAGAGACTG CCACCTACCA 
TCAGTCATGG AAATGACAAG TGGTGCATGG AAGTGCATGT TGTGCCAGGA TCTCGTGCCC 
AAAAACGATC AAGGCGTCGA CATGATTCAG TCACCAGCTG TAGATTCCAG CCGTCTGTCT 
CCGTCTGATC AGAAGAAATG TGAACGTCTG TTGTTGGAAA TGCTTTGTCA TGAGCCGTGC 
CGGCCCTTCC ACAGGTTATC CTCCAGTTCA GAAAATGATC CTATGATTGA CCTTACATTT 
ATTCGTGCCA GACTGCAGCA GAAACGCTCC CCTTCATATA GTACACCTGA AGACTTTGTT 
AGAGACGTGT GGATGATGTT CAGAAGCGTG AGCAAACTTG CAGAGGACAA AGCTGTGGTG 
CAGTCGATCA TTGAGCTGCA GTCTTTCTTC GAAACAAGAC TTAACCACCT TTTTGGTGAC 
CGCAAGTTCT CCTGTCTCCT GGGGGAGTTT TTAAGTGAGG AGGAGCAGCA AAGCGTTACA 
CAAAGAAGTT CCCCGATGAG CACAACCAGC GAGAGTGTAT CATCTGTCAA AAACACCACT 
GAGCAGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916264.1
CDS	300..2711
Translation	MSVAATRDKRSVLSTDNGNGGSGEPDGQELLESCGVCKKQLRADTEPQLLPCLHSVCRACLPPETPAPEVATNSGDLASAVVNCPICKHQCFQKDIVENYFLKVEPTSEPVTETIQRCTSCDDNTVANSYCVECTEWLCETCVEAHQRVKYTKDHTIKVKSSSKKETERAVYCPLHKNEPLMLYCESCDTLTCRDCQLQGHKDHQYQFLEDAVKNQRKVLSALVKRLGDKHTALQKSTKDVRTSIRQVSDLQKRLQVDVKMAILHIMKELNKRGKLLVNDMQKVTEGHQDKLEKQHWAMNKLQKHQEHILRFATWALESDNNIALLLSKKLIHFQLHRALKVIVDPVEPLGDLKFQWDSDTWTKHAEFFGKIVCDKTGVPQSSSGSLAAQSINSASSQVLASLTNFNPMVQNRPRLGASQIQASPARAGTAGNFIQPMQMMDDFDGSSAEGESSGFDTFPGQKRGRSSEGDVNELLKKVPRVSLERLDVDLNPDTQPPVFKVFPGNTNEDYNLIVIERGNLPGEAVVKEEAMETGIPIPTSDRSEKSLVIRPDGSTETVPPVIRQPTAAGNATSSDFQGASSGGFSDGLSGARCKVCQKEGALLTCSQCRSLFHRDCHLPSVMEMTSGAWKCMLCQDLVPKNDQGVDMIQSPAVDSSRLSPSDQKKCERLLLEMLCHEPCRPFHRLSSSSENDPMIDLTFIRARLQQKRSPSYSTPEDFVRDVWMMFRSVSKLAEDKAVVQSIIELQSFFETRLNHLFGDRKFSCLLGEFLSEEEQQSVTQRSSPMSTTSESVSSVKNTTEQD

Target ORF information:

RefSeq Version	XM_004916207.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916207.3

ORF Insert Sequence:

ATGTCGGTAG CTGCAACGCG TGACAAGCGG AGTGTCCTGA GCACCGACAA TGGCAACGGA 
GGCAGCGGGG AGCCCGACGG GCAGGAGCTA CTGGAGAGCT GCGGGGTGTG CAAGAAGCAG 
TTACGGGCGG ACACGGAGCC CCAGCTCTTA CCGTGCCTGC ACTCCGTGTG CCGGGCCTGC 
CTTCCGCCAG AGACCCCTGC GCCTGAGGTG GCCACTAACA GCGGAGACTT GGCTTCAGCT 
GTAGTTAATT GCCCCATATG CAAGCACCAG TGCTTCCAGA AGGATATTGT TGAGAACTAC 
TTTCTAAAAG TGGAACCCAC TTCCGAACCC GTAACTGAAA CAATTCAGAG ATGTACCAGC 
TGTGATGACA ATACTGTAGC AAATAGTTAC TGCGTCGAGT GCACAGAGTG GCTATGTGAG 
ACCTGTGTGG AGGCGCATCA GCGAGTGAAA TACACAAAGG ATCACACCAT CAAAGTTAAA 
AGTTCTTCCA AAAAGGAAAC AGAGCGAGCA GTTTACTGTC CTTTGCATAA AAATGAACCT 
CTCATGCTTT ATTGTGAATC CTGTGATACC TTAACTTGCC GGGACTGCCA ACTACAGGGA 
CACAAAGATC ACCAGTACCA GTTTTTAGAG GATGCTGTTA AAAATCAAAG GAAGGTACTT 
TCTGCTCTGG TCAAGCGGCT TGGAGATAAG CATACGGCTT TACAGAAATC TACTAAAGAT 
GTTCGAACTT CGATTCGACA GGTGAGTGAT TTACAGAAGA GATTGCAAGT GGATGTAAAG 
ATGGCAATCT TGCACATAAT GAAGGAACTT AATAAACGTG GAAAACTACT AGTGAATGAT 
ATGCAGAAAG TAACAGAAGG TCATCAGGAT AAACTGGAAA AGCAGCACTG GGCTATGAAC 
AAATTGCAAA AGCATCAAGA GCACATCCTT AGGTTTGCCA CGTGGGCACT GGAAAGTGAC 
AACAACATAG CCTTATTGCT TTCAAAGAAA CTGATACATT TTCAGTTGCA TCGTGCTCTA 
AAAGTTATTG TAGATCCAGT TGAGCCTTTA GGTGATTTAA AGTTCCAGTG GGACTCTGAT 
ACCTGGACAA AGCATGCAGA GTTTTTTGGC AAAATTGTAT GCGACAAAAC AGGTGTTCCT 
CAGTCATCTT CTGGATCGTT GGCAGCCCAG TCTATAAATT CTGCGTCATC TCAGGTGCTG 
GCTTCTCTTA CTAACTTTAA TCCAATGGTA CAGAATCGAC CAAGGCTTGG TGCTTCCCAG 
ATTCAAGCAT CTCCAGCCAG AGCTGGTACA GCTGGTAACT TCATCCAGCC CATGCAGATG 
ATGGATGACT TTGATGGGTC TTCTGCAGAG GGAGAATCTT CTGGATTTGA TACTTTCCCT 
GGCCAGAAAA GAGGGCGCAG TTCTGAAGGT GATGTAAATG AGCTTTTAAA AAAAGTTCCA 
CGTGTCAGTT TAGAGCGCTT GGATGTAGAC CTTAACCCCG ACACTCAACC ACCAGTCTTC 
AAAGTTTTCC CCGGAAACAC AAATGAAGAT TATAATTTAA TTGTAATTGA GAGAGGGAAT 
CTTCCAGGGG AAGCTGTTGT GAAGGAAGAA GCCATGGAAA CAGGCATCCC TATCCCAACA 
TCTGACCGCT CTGAAAAATC CTTAGTTATT CGACCAGATG GCTCGACAGA AACTGTGCCA 
CCTGTAATCC GCCAGCCTAC TGCAGCTGGT AATGCCACTT CCTCTGATTT CCAAGGAGCA 
AGCAGCGGGG GATTTTCTGA TGGGCTCAGT GGTGCCCGTT GTAAAGTGTG CCAGAAGGAA 
GGAGCCCTAC TGACATGTAG CCAATGTAGA AGCCTTTTCC ACAGAGACTG CCACCTACCA 
TCAGTCATGG AAATGACAAG TGGTGCATGG AAGTGCATGT TGTGCCAGGA TCTCGTGCCC 
AAAAACGATC AAGGCGTCGA CATGATTCAG TCACCAGCTG TAGATTCCAG CCGTCTGTCT 
CCGTCTGATC AGAAGAAATG TGAACGTCTG TTGTTGGAAA TGCTTTGTCA TGAGCCGTGC 
CGGCCCTTCC ACAGGTTATC CTCCAGTTCA GAAAATGATC CTATGATTGA CCTTACATTT 
ATTCGTGCCA GACTGCAGCA GAAACGCTCC CCTTCATATA GTACACCTGA AGACTTTGTT 
AGAGACGTGT GGATGATGTT CAGAAGCGTG AGCAAACTTG CAGAGGACAA AGCTGTGGTG 
CAGTCGATCA TTGAGCTGCA GTCTTTCTTC GAAACAAGAC TTAACCACCT TTTTGGTGAC 
CGCAAGTTCT CCTGTCTCCT GGGGGAGTTT TTAAGTGAGG AGGAGCAGCA AAGCGTTACA 
CAAAGAAGTT CCCCGATGAG CACAACCAGC GAGAGTGTAT CATCTGTCAA AAACACCACT 
GAGCAGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim28 ( XM_002937602.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937648.1 Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa13279
Clone ID Related Accession (Same CDS sequence)	XM_002937602.4 , XM_002937602.5
Accession Version	XM_002937602.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription intermediary factor 1-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937602.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTAG CTGCAACGCG TGACAAGCGG AGTGTCCTGA GCACCGACAA TGGCAACGGA 
GGCAGCGGGG AGCCCGACGG GCAGGAGCTA CTGGAGAGCT GCGGGGTGTG CAAGAAGCAG 
TTACGGGCGG ACACGGAGCC CCAGCTCTTA CCGTGCCTGC ACTCCGTGTG CCGGGCCTGC 
CTTCCGCCAG AGACCCCTGC GCCTGAGGTG GCCACTAACA GCGGAGACTT GGCTTCAGCT 
GTAGTTAATT GCCCCATATG CAAGCACCAG TGCTTCCAGA AGGATATTGT TGAGAACTAC 
TTTCTAAAAG TGGAACCCAC TTCCGAACCC GTAACTGAAA CAATTCAGAG ATGTACCAGC 
TGTGATGACA ATACTGTAGC AAATAGTTAC TGCGTCGAGT GCACAGAGTG GCTATGTGAG 
ACCTGTGTGG AGGCGCATCA GCGAGTGAAA TACACAAAGG ATCACACCAT CAAAGTTAAA 
AGTTCTTCCA AAAAGGAAAC AGAGCGAGCA GTTTACTGTC CTTTGCATAA AAATGAACCT 
CTCATGCTTT ATTGTGAATC CTGTGATACC TTAACTTGCC GGGACTGCCA ACTACAGGGA 
CACAAAGATC ACCAGTACCA GTTTTTAGAG GATGCTGTTA AAAATCAAAG GAAGGTACTT 
TCTGCTCTGG TCAAGCGGCT TGGAGATAAG CATACGGCTT TACAGAAATC TACTAAAGAT 
GTTCGAACTT CGATTCGACA GGTGAGTGAT TTACAGAAGA GATTGCAAGT GGATGTAAAG 
ATGGCAATCT TGCACATAAT GAAGGAACTT AATAAACGTG GAAAACTACT AGTGAATGAT 
ATGCAGAAAG TAACAGAAGG TCATCAGGAT AAACTGGAAA AGCAGCACTG GGCTATGAAC 
AAATTGCAAA AGCATCAAGA GCACATCCTT AGGTTTGCCA CGTGGGCACT GGAAAGTGAC 
AACAACATAG CCTTATTGCT TTCAAAGAAA CTGATACATT TTCAGTTGCA TCGTGCTCTA 
AAAGTTATTG TAGATCCAGT TGAGCCTTTA GGTGATTTAA AGTTCCAGTG GGACTCTGAT 
ACCTGGACAA AGCATGCAGA GTTTTTTGGC AAAATTGTAT GCGACAAAAC AGGTGTTCCT 
CAGTCATCTT CTGGATCGTT GGCAGCCCAG TCTATAAATT CTGCGTCATC TCAGATGATG 
GATGACTTTG ATGGGTCTTC TGCAGAGGGA GAATCTTCTG GATTTGATAC TTTCCCTGGC 
CAGAAAAGAG GGCGCAGTTC TGAAGGTGAT GTAAATGAGC TTTTAAAAAA AGTTCCACGT 
GTCAGTTTAG AGCGCTTGGA TGTAGACCTT AACCCCGACA CTCAACCACC AGTCTTCAAA 
GTTTTCCCCG GAAACACAAA TGAAGATTAT AATTTAATTG TAATTGAGAG AGGGAATCTT 
CCAGGGGAAG CTGTTGTGAA GGAAGAAGCC ATGGAAACAG GCATCCCTAT CCCAACATCT 
GACCGCTCTG AAAAATCCTT AGTTATTCGA CCAGATGGCT CGACAGAAAC TGTGCCACCT 
GTAATCCGCC AGCCTACTGC AGCTGGTAAT GCCACTTCCT CTGATTTCCA AGGAGCAAGC 
AGCGGGGGAT TTTCTGATGG GCTCAGTGGT GCCCGTTGTA AAGTGTGCCA GAAGGAAGGA 
GCCCTACTGA CATGTAGCCA ATGTAGAAGC CTTTTCCACA GAGACTGCCA CCTACCATCA 
GTCATGGAAA TGACAAGTGG TGCATGGAAG TGCATGTTGT GCCAGGATCT CGTGCCCAAA 
AACGATCAAG GCGTCGACAT GATTCAGTCA CCAGCTGTAG ATTCCAGCCG TCTGTCTCCG 
TCTGATCAGA AGAAATGTGA ACGTCTGTTG TTGGAAATGC TTTGTCATGA GCCGTGCCGG 
CCCTTCCACA GGTTATCCTC CAGTTCAGAA AATGATCCTA TGATTGACCT TACATTTATT 
CGTGCCAGAC TGCAGCAGAA ACGCTCCCCT TCATATAGTA CACCTGAAGA CTTTGTTAGA 
GACGTGTGGA TGATGTTCAG AAGCGTGAGC AAACTTGCAG AGGACAAAGC TGTGGTGCAG 
TCGATCATTG AGCTGCAGTC TTTCTTCGAA ACAAGACTTA ACCACCTTTT TGGTGACCGC 
AAGTTCTCCT GTCTCCTGGG GGAGTTTTTA AGTGAGGAGG AGCAGCAAAG CGTTACACAA 
AGAAGTTCCC CGATGAGCAC AACCAGCGAG AGTGTATCAT CTGTCAAAAA CACCACTGAG 
CAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937648.1
CDS	298..2586
Translation	MSVAATRDKRSVLSTDNGNGGSGEPDGQELLESCGVCKKQLRADTEPQLLPCLHSVCRACLPPETPAPEVATNSGDLASAVVNCPICKHQCFQKDIVENYFLKVEPTSEPVTETIQRCTSCDDNTVANSYCVECTEWLCETCVEAHQRVKYTKDHTIKVKSSSKKETERAVYCPLHKNEPLMLYCESCDTLTCRDCQLQGHKDHQYQFLEDAVKNQRKVLSALVKRLGDKHTALQKSTKDVRTSIRQVSDLQKRLQVDVKMAILHIMKELNKRGKLLVNDMQKVTEGHQDKLEKQHWAMNKLQKHQEHILRFATWALESDNNIALLLSKKLIHFQLHRALKVIVDPVEPLGDLKFQWDSDTWTKHAEFFGKIVCDKTGVPQSSSGSLAAQSINSASSQMMDDFDGSSAEGESSGFDTFPGQKRGRSSEGDVNELLKKVPRVSLERLDVDLNPDTQPPVFKVFPGNTNEDYNLIVIERGNLPGEAVVKEEAMETGIPIPTSDRSEKSLVIRPDGSTETVPPVIRQPTAAGNATSSDFQGASSGGFSDGLSGARCKVCQKEGALLTCSQCRSLFHRDCHLPSVMEMTSGAWKCMLCQDLVPKNDQGVDMIQSPAVDSSRLSPSDQKKCERLLLEMLCHEPCRPFHRLSSSSENDPMIDLTFIRARLQQKRSPSYSTPEDFVRDVWMMFRSVSKLAEDKAVVQSIIELQSFFETRLNHLFGDRKFSCLLGEFLSEEEQQSVTQRSSPMSTTSESVSSVKNTTEQD

Target ORF information:

RefSeq Version	XM_002937602.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937602.4

ORF Insert Sequence:

ATGTCGGTAG CTGCAACGCG TGACAAGCGG AGTGTCCTGA GCACCGACAA TGGCAACGGA 
GGCAGCGGGG AGCCCGACGG GCAGGAGCTA CTGGAGAGCT GCGGGGTGTG CAAGAAGCAG 
TTACGGGCGG ACACGGAGCC CCAGCTCTTA CCGTGCCTGC ACTCCGTGTG CCGGGCCTGC 
CTTCCGCCAG AGACCCCTGC GCCTGAGGTG GCCACTAACA GCGGAGACTT GGCTTCAGCT 
GTAGTTAATT GCCCCATATG CAAGCACCAG TGCTTCCAGA AGGATATTGT TGAGAACTAC 
TTTCTAAAAG TGGAACCCAC TTCCGAACCC GTAACTGAAA CAATTCAGAG ATGTACCAGC 
TGTGATGACA ATACTGTAGC AAATAGTTAC TGCGTCGAGT GCACAGAGTG GCTATGTGAG 
ACCTGTGTGG AGGCGCATCA GCGAGTGAAA TACACAAAGG ATCACACCAT CAAAGTTAAA 
AGTTCTTCCA AAAAGGAAAC AGAGCGAGCA GTTTACTGTC CTTTGCATAA AAATGAACCT 
CTCATGCTTT ATTGTGAATC CTGTGATACC TTAACTTGCC GGGACTGCCA ACTACAGGGA 
CACAAAGATC ACCAGTACCA GTTTTTAGAG GATGCTGTTA AAAATCAAAG GAAGGTACTT 
TCTGCTCTGG TCAAGCGGCT TGGAGATAAG CATACGGCTT TACAGAAATC TACTAAAGAT 
GTTCGAACTT CGATTCGACA GGTGAGTGAT TTACAGAAGA GATTGCAAGT GGATGTAAAG 
ATGGCAATCT TGCACATAAT GAAGGAACTT AATAAACGTG GAAAACTACT AGTGAATGAT 
ATGCAGAAAG TAACAGAAGG TCATCAGGAT AAACTGGAAA AGCAGCACTG GGCTATGAAC 
AAATTGCAAA AGCATCAAGA GCACATCCTT AGGTTTGCCA CGTGGGCACT GGAAAGTGAC 
AACAACATAG CCTTATTGCT TTCAAAGAAA CTGATACATT TTCAGTTGCA TCGTGCTCTA 
AAAGTTATTG TAGATCCAGT TGAGCCTTTA GGTGATTTAA AGTTCCAGTG GGACTCTGAT 
ACCTGGACAA AGCATGCAGA GTTTTTTGGC AAAATTGTAT GCGACAAAAC AGGTGTTCCT 
CAGTCATCTT CTGGATCGTT GGCAGCCCAG TCTATAAATT CTGCGTCATC TCAGATGATG 
GATGACTTTG ATGGGTCTTC TGCAGAGGGA GAATCTTCTG GATTTGATAC TTTCCCTGGC 
CAGAAAAGAG GGCGCAGTTC TGAAGGTGAT GTAAATGAGC TTTTAAAAAA AGTTCCACGT 
GTCAGTTTAG AGCGCTTGGA TGTAGACCTT AACCCCGACA CTCAACCACC AGTCTTCAAA 
GTTTTCCCCG GAAACACAAA TGAAGATTAT AATTTAATTG TAATTGAGAG AGGGAATCTT 
CCAGGGGAAG CTGTTGTGAA GGAAGAAGCC ATGGAAACAG GCATCCCTAT CCCAACATCT 
GACCGCTCTG AAAAATCCTT AGTTATTCGA CCAGATGGCT CGACAGAAAC TGTGCCACCT 
GTAATCCGCC AGCCTACTGC AGCTGGTAAT GCCACTTCCT CTGATTTCCA AGGAGCAAGC 
AGCGGGGGAT TTTCTGATGG GCTCAGTGGT GCCCGTTGTA AAGTGTGCCA GAAGGAAGGA 
GCCCTACTGA CATGTAGCCA ATGTAGAAGC CTTTTCCACA GAGACTGCCA CCTACCATCA 
GTCATGGAAA TGACAAGTGG TGCATGGAAG TGCATGTTGT GCCAGGATCT CGTGCCCAAA 
AACGATCAAG GCGTCGACAT GATTCAGTCA CCAGCTGTAG ATTCCAGCCG TCTGTCTCCG 
TCTGATCAGA AGAAATGTGA ACGTCTGTTG TTGGAAATGC TTTGTCATGA GCCGTGCCGG 
CCCTTCCACA GGTTATCCTC CAGTTCAGAA AATGATCCTA TGATTGACCT TACATTTATT 
CGTGCCAGAC TGCAGCAGAA ACGCTCCCCT TCATATAGTA CACCTGAAGA CTTTGTTAGA 
GACGTGTGGA TGATGTTCAG AAGCGTGAGC AAACTTGCAG AGGACAAAGC TGTGGTGCAG 
TCGATCATTG AGCTGCAGTC TTTCTTCGAA ACAAGACTTA ACCACCTTTT TGGTGACCGC 
AAGTTCTCCT GTCTCCTGGG GGAGTTTTTA AGTGAGGAGG AGCAGCAAAG CGTTACACAA 
AGAAGTTCCC CGATGAGCAC AACCAGCGAG AGTGTATCAT CTGTCAAAAA CACCACTGAG 
CAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim28 ( XM_002937602.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937648.1 Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X1, mRNA.

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CloneID	OXa13279
Clone ID Related Accession (Same CDS sequence)	XM_002937602.4 , XM_002937602.5
Accession Version	XM_002937602.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription intermediary factor 1-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937602.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTAG CTGCAACGCG TGACAAGCGG AGTGTCCTGA GCACCGACAA TGGCAACGGA 
GGCAGCGGGG AGCCCGACGG GCAGGAGCTA CTGGAGAGCT GCGGGGTGTG CAAGAAGCAG 
TTACGGGCGG ACACGGAGCC CCAGCTCTTA CCGTGCCTGC ACTCCGTGTG CCGGGCCTGC 
CTTCCGCCAG AGACCCCTGC GCCTGAGGTG GCCACTAACA GCGGAGACTT GGCTTCAGCT 
GTAGTTAATT GCCCCATATG CAAGCACCAG TGCTTCCAGA AGGATATTGT TGAGAACTAC 
TTTCTAAAAG TGGAACCCAC TTCCGAACCC GTAACTGAAA CAATTCAGAG ATGTACCAGC 
TGTGATGACA ATACTGTAGC AAATAGTTAC TGCGTCGAGT GCACAGAGTG GCTATGTGAG 
ACCTGTGTGG AGGCGCATCA GCGAGTGAAA TACACAAAGG ATCACACCAT CAAAGTTAAA 
AGTTCTTCCA AAAAGGAAAC AGAGCGAGCA GTTTACTGTC CTTTGCATAA AAATGAACCT 
CTCATGCTTT ATTGTGAATC CTGTGATACC TTAACTTGCC GGGACTGCCA ACTACAGGGA 
CACAAAGATC ACCAGTACCA GTTTTTAGAG GATGCTGTTA AAAATCAAAG GAAGGTACTT 
TCTGCTCTGG TCAAGCGGCT TGGAGATAAG CATACGGCTT TACAGAAATC TACTAAAGAT 
GTTCGAACTT CGATTCGACA GGTGAGTGAT TTACAGAAGA GATTGCAAGT GGATGTAAAG 
ATGGCAATCT TGCACATAAT GAAGGAACTT AATAAACGTG GAAAACTACT AGTGAATGAT 
ATGCAGAAAG TAACAGAAGG TCATCAGGAT AAACTGGAAA AGCAGCACTG GGCTATGAAC 
AAATTGCAAA AGCATCAAGA GCACATCCTT AGGTTTGCCA CGTGGGCACT GGAAAGTGAC 
AACAACATAG CCTTATTGCT TTCAAAGAAA CTGATACATT TTCAGTTGCA TCGTGCTCTA 
AAAGTTATTG TAGATCCAGT TGAGCCTTTA GGTGATTTAA AGTTCCAGTG GGACTCTGAT 
ACCTGGACAA AGCATGCAGA GTTTTTTGGC AAAATTGTAT GCGACAAAAC AGGTGTTCCT 
CAGTCATCTT CTGGATCGTT GGCAGCCCAG TCTATAAATT CTGCGTCATC TCAGATGATG 
GATGACTTTG ATGGGTCTTC TGCAGAGGGA GAATCTTCTG GATTTGATAC TTTCCCTGGC 
CAGAAAAGAG GGCGCAGTTC TGAAGGTGAT GTAAATGAGC TTTTAAAAAA AGTTCCACGT 
GTCAGTTTAG AGCGCTTGGA TGTAGACCTT AACCCCGACA CTCAACCACC AGTCTTCAAA 
GTTTTCCCCG GAAACACAAA TGAAGATTAT AATTTAATTG TAATTGAGAG AGGGAATCTT 
CCAGGGGAAG CTGTTGTGAA GGAAGAAGCC ATGGAAACAG GCATCCCTAT CCCAACATCT 
GACCGCTCTG AAAAATCCTT AGTTATTCGA CCAGATGGCT CGACAGAAAC TGTGCCACCT 
GTAATCCGCC AGCCTACTGC AGCTGGTAAT GCCACTTCCT CTGATTTCCA AGGAGCAAGC 
AGCGGGGGAT TTTCTGATGG GCTCAGTGGT GCCCGTTGTA AAGTGTGCCA GAAGGAAGGA 
GCCCTACTGA CATGTAGCCA ATGTAGAAGC CTTTTCCACA GAGACTGCCA CCTACCATCA 
GTCATGGAAA TGACAAGTGG TGCATGGAAG TGCATGTTGT GCCAGGATCT CGTGCCCAAA 
AACGATCAAG GCGTCGACAT GATTCAGTCA CCAGCTGTAG ATTCCAGCCG TCTGTCTCCG 
TCTGATCAGA AGAAATGTGA ACGTCTGTTG TTGGAAATGC TTTGTCATGA GCCGTGCCGG 
CCCTTCCACA GGTTATCCTC CAGTTCAGAA AATGATCCTA TGATTGACCT TACATTTATT 
CGTGCCAGAC TGCAGCAGAA ACGCTCCCCT TCATATAGTA CACCTGAAGA CTTTGTTAGA 
GACGTGTGGA TGATGTTCAG AAGCGTGAGC AAACTTGCAG AGGACAAAGC TGTGGTGCAG 
TCGATCATTG AGCTGCAGTC TTTCTTCGAA ACAAGACTTA ACCACCTTTT TGGTGACCGC 
AAGTTCTCCT GTCTCCTGGG GGAGTTTTTA AGTGAGGAGG AGCAGCAAAG CGTTACACAA 
AGAAGTTCCC CGATGAGCAC AACCAGCGAG AGTGTATCAT CTGTCAAAAA CACCACTGAG 
CAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937648.1
CDS	242..2530
Translation	MSVAATRDKRSVLSTDNGNGGSGEPDGQELLESCGVCKKQLRADTEPQLLPCLHSVCRACLPPETPAPEVATNSGDLASAVVNCPICKHQCFQKDIVENYFLKVEPTSEPVTETIQRCTSCDDNTVANSYCVECTEWLCETCVEAHQRVKYTKDHTIKVKSSSKKETERAVYCPLHKNEPLMLYCESCDTLTCRDCQLQGHKDHQYQFLEDAVKNQRKVLSALVKRLGDKHTALQKSTKDVRTSIRQVSDLQKRLQVDVKMAILHIMKELNKRGKLLVNDMQKVTEGHQDKLEKQHWAMNKLQKHQEHILRFATWALESDNNIALLLSKKLIHFQLHRALKVIVDPVEPLGDLKFQWDSDTWTKHAEFFGKIVCDKTGVPQSSSGSLAAQSINSASSQMMDDFDGSSAEGESSGFDTFPGQKRGRSSEGDVNELLKKVPRVSLERLDVDLNPDTQPPVFKVFPGNTNEDYNLIVIERGNLPGEAVVKEEAMETGIPIPTSDRSEKSLVIRPDGSTETVPPVIRQPTAAGNATSSDFQGASSGGFSDGLSGARCKVCQKEGALLTCSQCRSLFHRDCHLPSVMEMTSGAWKCMLCQDLVPKNDQGVDMIQSPAVDSSRLSPSDQKKCERLLLEMLCHEPCRPFHRLSSSSENDPMIDLTFIRARLQQKRSPSYSTPEDFVRDVWMMFRSVSKLAEDKAVVQSIIELQSFFETRLNHLFGDRKFSCLLGEFLSEEEQQSVTQRSSPMSTTSESVSSVKNTTEQD

Target ORF information:

RefSeq Version	XM_002937602.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937602.5

ORF Insert Sequence:

ATGTCGGTAG CTGCAACGCG TGACAAGCGG AGTGTCCTGA GCACCGACAA TGGCAACGGA 
GGCAGCGGGG AGCCCGACGG GCAGGAGCTA CTGGAGAGCT GCGGGGTGTG CAAGAAGCAG 
TTACGGGCGG ACACGGAGCC CCAGCTCTTA CCGTGCCTGC ACTCCGTGTG CCGGGCCTGC 
CTTCCGCCAG AGACCCCTGC GCCTGAGGTG GCCACTAACA GCGGAGACTT GGCTTCAGCT 
GTAGTTAATT GCCCCATATG CAAGCACCAG TGCTTCCAGA AGGATATTGT TGAGAACTAC 
TTTCTAAAAG TGGAACCCAC TTCCGAACCC GTAACTGAAA CAATTCAGAG ATGTACCAGC 
TGTGATGACA ATACTGTAGC AAATAGTTAC TGCGTCGAGT GCACAGAGTG GCTATGTGAG 
ACCTGTGTGG AGGCGCATCA GCGAGTGAAA TACACAAAGG ATCACACCAT CAAAGTTAAA 
AGTTCTTCCA AAAAGGAAAC AGAGCGAGCA GTTTACTGTC CTTTGCATAA AAATGAACCT 
CTCATGCTTT ATTGTGAATC CTGTGATACC TTAACTTGCC GGGACTGCCA ACTACAGGGA 
CACAAAGATC ACCAGTACCA GTTTTTAGAG GATGCTGTTA AAAATCAAAG GAAGGTACTT 
TCTGCTCTGG TCAAGCGGCT TGGAGATAAG CATACGGCTT TACAGAAATC TACTAAAGAT 
GTTCGAACTT CGATTCGACA GGTGAGTGAT TTACAGAAGA GATTGCAAGT GGATGTAAAG 
ATGGCAATCT TGCACATAAT GAAGGAACTT AATAAACGTG GAAAACTACT AGTGAATGAT 
ATGCAGAAAG TAACAGAAGG TCATCAGGAT AAACTGGAAA AGCAGCACTG GGCTATGAAC 
AAATTGCAAA AGCATCAAGA GCACATCCTT AGGTTTGCCA CGTGGGCACT GGAAAGTGAC 
AACAACATAG CCTTATTGCT TTCAAAGAAA CTGATACATT TTCAGTTGCA TCGTGCTCTA 
AAAGTTATTG TAGATCCAGT TGAGCCTTTA GGTGATTTAA AGTTCCAGTG GGACTCTGAT 
ACCTGGACAA AGCATGCAGA GTTTTTTGGC AAAATTGTAT GCGACAAAAC AGGTGTTCCT 
CAGTCATCTT CTGGATCGTT GGCAGCCCAG TCTATAAATT CTGCGTCATC TCAGATGATG 
GATGACTTTG ATGGGTCTTC TGCAGAGGGA GAATCTTCTG GATTTGATAC TTTCCCTGGC 
CAGAAAAGAG GGCGCAGTTC TGAAGGTGAT GTAAATGAGC TTTTAAAAAA AGTTCCACGT 
GTCAGTTTAG AGCGCTTGGA TGTAGACCTT AACCCCGACA CTCAACCACC AGTCTTCAAA 
GTTTTCCCCG GAAACACAAA TGAAGATTAT AATTTAATTG TAATTGAGAG AGGGAATCTT 
CCAGGGGAAG CTGTTGTGAA GGAAGAAGCC ATGGAAACAG GCATCCCTAT CCCAACATCT 
GACCGCTCTG AAAAATCCTT AGTTATTCGA CCAGATGGCT CGACAGAAAC TGTGCCACCT 
GTAATCCGCC AGCCTACTGC AGCTGGTAAT GCCACTTCCT CTGATTTCCA AGGAGCAAGC 
AGCGGGGGAT TTTCTGATGG GCTCAGTGGT GCCCGTTGTA AAGTGTGCCA GAAGGAAGGA 
GCCCTACTGA CATGTAGCCA ATGTAGAAGC CTTTTCCACA GAGACTGCCA CCTACCATCA 
GTCATGGAAA TGACAAGTGG TGCATGGAAG TGCATGTTGT GCCAGGATCT CGTGCCCAAA 
AACGATCAAG GCGTCGACAT GATTCAGTCA CCAGCTGTAG ATTCCAGCCG TCTGTCTCCG 
TCTGATCAGA AGAAATGTGA ACGTCTGTTG TTGGAAATGC TTTGTCATGA GCCGTGCCGG 
CCCTTCCACA GGTTATCCTC CAGTTCAGAA AATGATCCTA TGATTGACCT TACATTTATT 
CGTGCCAGAC TGCAGCAGAA ACGCTCCCCT TCATATAGTA CACCTGAAGA CTTTGTTAGA 
GACGTGTGGA TGATGTTCAG AAGCGTGAGC AAACTTGCAG AGGACAAAGC TGTGGTGCAG 
TCGATCATTG AGCTGCAGTC TTTCTTCGAA ACAAGACTTA ACCACCTTTT TGGTGACCGC 
AAGTTCTCCT GTCTCCTGGG GGAGTTTTTA AGTGAGGAGG AGCAGCAAAG CGTTACACAA 
AGAAGTTCCC CGATGAGCAC AACCAGCGAG AGTGTATCAT CTGTCAAAAA CACCACTGAG 
CAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim28 ( XM_012967404.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822858.1 Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X4, mRNA.

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CloneID	OXa34567
Clone ID Related Accession (Same CDS sequence)	XM_012967404.2
Accession Version	XM_012967404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription intermediary factor 1-beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATGTA CCAGCTGTGA TGACAATACT GTAGCAAATA GTTACTGCGT CGAGTGCACA 
GAGTGGCTAT GTGAGACCTG TGTGGAGGCG CATCAGCGAG TGAAATACAC AAAGGATCAC 
ACCATCAAAG TTAAAAGTTC TTCCAAAAAG GAAACAGAGC GAGCAGTTTA CTGTCCTTTG 
CATAAAAATG AACCTCTCAT GCTTTATTGT GAATCCTGTG ATACCTTAAC TTGCCGGGAC 
TGCCAACTAC AGGGACACAA AGATCACCAG TACCAGTTTT TAGAGGATGC TGTTAAAAAT 
CAAAGGAAGG TACTTTCTGC TCTGGTCAAG CGGCTTGGAG ATAAGCATAC GGCTTTACAG 
AAATCTACTA AAGATGTTCG AACTTCGATT CGACAGGTGA GTGATTTACA GAAGAGATTG 
CAAGTGGATG TAAAGATGGC AATCTTGCAC ATAATGAAGG AACTTAATAA ACGTGGAAAA 
CTACTAGTGA ATGATATGCA GAAAGTAACA GAAGGTCATC AGGATAAACT GGAAAAGCAG 
CACTGGGCTA TGAACAAATT GCAAAAGCAT CAAGAGCACA TCCTTAGGTT TGCCACGTGG 
GCACTGGAAA GTGACAACAA CATAGCCTTA TTGCTTTCAA AGAAACTGAT ACATTTTCAG 
TTGCATCGTG CTCTAAAAGT TATTGTAGAT CCAGTTGAGC CTTTAGGTGA TTTAAAGTTC 
CAGTGGGACT CTGATACCTG GACAAAGCAT GCAGAGTTTT TTGGCAAAAT TGTATGCGAC 
AAAACAGGTG TTCCTCAGTC ATCTTCTGGA TCGTTGGCAG CCCAGTCTAT AAATTCTGCG 
TCATCTCAGG TGCTGGCTTC TCTTACTAAC TTTAATCCAA TGGTACAGAA TCGACCAAGG 
CTTGGTGCTT CCCAGATTCA AGCATCTCCA GCCAGAGCTG GTACAGCTGG TAACTTCATC 
CAGCCCATGC AGATGATGGA TGACTTTGAT GGGTCTTCTG CAGAGGGAGA ATCTTCTGGA 
TTTGATACTT TCCCTGGCCA GAAAAGAGGG CGCAGTTCTG AAGGTGATGT AAATGAGCTT 
TTAAAAAAAG TTCCACGTGT CAGTTTAGAG CGCTTGGATG TAGACCTTAA CCCCGACACT 
CAACCACCAG TCTTCAAAGT TTTCCCCGGA AACACAAATG AAGATTATAA TTTAATTGTA 
ATTGAGAGAG GGAATCTTCC AGGGGAAGCT GTTGTGAAGG AAGAAGCCAT GGAAACAGGC 
ATCCCTATCC CAACATCTGA CCGCTCTGAA AAATCCTTAG TTATTCGACC AGATGGCTCG 
ACAGAAACTG TGCCACCTGT AATCCGCCAG CCTACTGCAG CTGGTAATGC CACTTCCTCT 
GATTTCCAAG GAGCAAGCAG CGGGGGATTT TCTGATGGGC TCAGTGGTGC CCGTTGTAAA 
GTGTGCCAGA AGGAAGGAGC CCTACTGACA TGTAGCCAAT GTAGAAGCCT TTTCCACAGA 
GACTGCCACC TACCATCAGT CATGGAAATG ACAAGTGGTG CATGGAAGTG CATGTTGTGC 
CAGGATCTCG TGCCCAAAAA CGATCAAGGC GTCGACATGA TTCAGTCACC AGCTGTAGAT 
TCCAGCCGTC TGTCTCCGTC TGATCAGAAG AAATGTGAAC GTCTGTTGTT GGAAATGCTT 
TGTCATGAGC CGTGCCGGCC CTTCCACAGG TTATCCTCCA GTTCAGAAAA TGATCCTATG 
ATTGACCTTA CATTTATTCG TGCCAGACTG CAGCAGAAAC GCTCCCCTTC ATATAGTACA 
CCTGAAGACT TTGTTAGAGA CGTGTGGATG ATGTTCAGAA GCGTGAGCAA ACTTGCAGAG 
GACAAAGCTG TGGTGCAGTC GATCATTGAG CTGCAGTCTT TCTTCGAAAC AAGACTTAAC 
CACCTTTTTG GTGACCGCAA GTTCTCCTGT CTCCTGGGGG AGTTTTTAAG TGAGGAGGAG 
CAGCAAAGCG TTACACAAAG AAGTTCCCCG ATGAGCACAA CCAGCGAGAG TGTATCATCT 
GTCAAAAACA CCACTGAGCA GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822858.1
CDS	367..2433
Translation	MRCTSCDDNTVANSYCVECTEWLCETCVEAHQRVKYTKDHTIKVKSSSKKETERAVYCPLHKNEPLMLYCESCDTLTCRDCQLQGHKDHQYQFLEDAVKNQRKVLSALVKRLGDKHTALQKSTKDVRTSIRQVSDLQKRLQVDVKMAILHIMKELNKRGKLLVNDMQKVTEGHQDKLEKQHWAMNKLQKHQEHILRFATWALESDNNIALLLSKKLIHFQLHRALKVIVDPVEPLGDLKFQWDSDTWTKHAEFFGKIVCDKTGVPQSSSGSLAAQSINSASSQVLASLTNFNPMVQNRPRLGASQIQASPARAGTAGNFIQPMQMMDDFDGSSAEGESSGFDTFPGQKRGRSSEGDVNELLKKVPRVSLERLDVDLNPDTQPPVFKVFPGNTNEDYNLIVIERGNLPGEAVVKEEAMETGIPIPTSDRSEKSLVIRPDGSTETVPPVIRQPTAAGNATSSDFQGASSGGFSDGLSGARCKVCQKEGALLTCSQCRSLFHRDCHLPSVMEMTSGAWKCMLCQDLVPKNDQGVDMIQSPAVDSSRLSPSDQKKCERLLLEMLCHEPCRPFHRLSSSSENDPMIDLTFIRARLQQKRSPSYSTPEDFVRDVWMMFRSVSKLAEDKAVVQSIIELQSFFETRLNHLFGDRKFSCLLGEFLSEEEQQSVTQRSSPMSTTSESVSSVKNTTEQD

Target ORF information:

RefSeq Version	XM_012967404.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967404.2

ORF Insert Sequence:

ATGAGATGTA CCAGCTGTGA TGACAATACT GTAGCAAATA GTTACTGCGT CGAGTGCACA 
GAGTGGCTAT GTGAGACCTG TGTGGAGGCG CATCAGCGAG TGAAATACAC AAAGGATCAC 
ACCATCAAAG TTAAAAGTTC TTCCAAAAAG GAAACAGAGC GAGCAGTTTA CTGTCCTTTG 
CATAAAAATG AACCTCTCAT GCTTTATTGT GAATCCTGTG ATACCTTAAC TTGCCGGGAC 
TGCCAACTAC AGGGACACAA AGATCACCAG TACCAGTTTT TAGAGGATGC TGTTAAAAAT 
CAAAGGAAGG TACTTTCTGC TCTGGTCAAG CGGCTTGGAG ATAAGCATAC GGCTTTACAG 
AAATCTACTA AAGATGTTCG AACTTCGATT CGACAGGTGA GTGATTTACA GAAGAGATTG 
CAAGTGGATG TAAAGATGGC AATCTTGCAC ATAATGAAGG AACTTAATAA ACGTGGAAAA 
CTACTAGTGA ATGATATGCA GAAAGTAACA GAAGGTCATC AGGATAAACT GGAAAAGCAG 
CACTGGGCTA TGAACAAATT GCAAAAGCAT CAAGAGCACA TCCTTAGGTT TGCCACGTGG 
GCACTGGAAA GTGACAACAA CATAGCCTTA TTGCTTTCAA AGAAACTGAT ACATTTTCAG 
TTGCATCGTG CTCTAAAAGT TATTGTAGAT CCAGTTGAGC CTTTAGGTGA TTTAAAGTTC 
CAGTGGGACT CTGATACCTG GACAAAGCAT GCAGAGTTTT TTGGCAAAAT TGTATGCGAC 
AAAACAGGTG TTCCTCAGTC ATCTTCTGGA TCGTTGGCAG CCCAGTCTAT AAATTCTGCG 
TCATCTCAGG TGCTGGCTTC TCTTACTAAC TTTAATCCAA TGGTACAGAA TCGACCAAGG 
CTTGGTGCTT CCCAGATTCA AGCATCTCCA GCCAGAGCTG GTACAGCTGG TAACTTCATC 
CAGCCCATGC AGATGATGGA TGACTTTGAT GGGTCTTCTG CAGAGGGAGA ATCTTCTGGA 
TTTGATACTT TCCCTGGCCA GAAAAGAGGG CGCAGTTCTG AAGGTGATGT AAATGAGCTT 
TTAAAAAAAG TTCCACGTGT CAGTTTAGAG CGCTTGGATG TAGACCTTAA CCCCGACACT 
CAACCACCAG TCTTCAAAGT TTTCCCCGGA AACACAAATG AAGATTATAA TTTAATTGTA 
ATTGAGAGAG GGAATCTTCC AGGGGAAGCT GTTGTGAAGG AAGAAGCCAT GGAAACAGGC 
ATCCCTATCC CAACATCTGA CCGCTCTGAA AAATCCTTAG TTATTCGACC AGATGGCTCG 
ACAGAAACTG TGCCACCTGT AATCCGCCAG CCTACTGCAG CTGGTAATGC CACTTCCTCT 
GATTTCCAAG GAGCAAGCAG CGGGGGATTT TCTGATGGGC TCAGTGGTGC CCGTTGTAAA 
GTGTGCCAGA AGGAAGGAGC CCTACTGACA TGTAGCCAAT GTAGAAGCCT TTTCCACAGA 
GACTGCCACC TACCATCAGT CATGGAAATG ACAAGTGGTG CATGGAAGTG CATGTTGTGC 
CAGGATCTCG TGCCCAAAAA CGATCAAGGC GTCGACATGA TTCAGTCACC AGCTGTAGAT 
TCCAGCCGTC TGTCTCCGTC TGATCAGAAG AAATGTGAAC GTCTGTTGTT GGAAATGCTT 
TGTCATGAGC CGTGCCGGCC CTTCCACAGG TTATCCTCCA GTTCAGAAAA TGATCCTATG 
ATTGACCTTA CATTTATTCG TGCCAGACTG CAGCAGAAAC GCTCCCCTTC ATATAGTACA 
CCTGAAGACT TTGTTAGAGA CGTGTGGATG ATGTTCAGAA GCGTGAGCAA ACTTGCAGAG 
GACAAAGCTG TGGTGCAGTC GATCATTGAG CTGCAGTCTT TCTTCGAAAC AAGACTTAAC 
CACCTTTTTG GTGACCGCAA GTTCTCCTGT CTCCTGGGGG AGTTTTTAAG TGAGGAGGAG 
CAGCAAAGCG TTACACAAAG AAGTTCCCCG ATGAGCACAA CCAGCGAGAG TGTATCATCT 
GTCAAAAACA CCACTGAGCA GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim28 ( XM_018095471.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950960.1 Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X3, mRNA.

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CloneID	OXa45438
Clone ID Related Accession (Same CDS sequence)	XM_018095471.1
Accession Version	XM_018095471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription intermediary factor 1-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACGG AGGCAGCGGG GAGCCCGACG GGCAGGAGCT ACTGGAGAGC TGCGGGGTGT 
GCAAGAAGCA GTTACGGGCG GACACGGAGC CCCAGCTCTT ACCGTGCCTG CACTCCGTGT 
GCCGGGCCTG CCTTCCGCCA GAGACCCCTG CGCCTGAGGT GGCCACTAAC AGCGGAGACT 
TGGCTTCAGA GATGTACCAG CTGTGATGAC AATACTGTAG CAAATAGTTA CTGCGTCGAG 
TGCACAGAGT GGCTATGTGA GACCTGTGTG GAGGCGCATC AGCGAGTGAA ATACACAAAG 
GATCACACCA TCAAAGTTAA AAGTTCTTCC AAAAAGGAAA CAGAGCGAGC AGTTTACTGT 
CCTTTGCATA AAAATGAACC TCTCATGCTT TATTGTGAAT CCTGTGATAC CTTAACTTGC 
CGGGACTGCC AACTACAGGG ACACAAAGAT CACCAGTACC AGTTTTTAGA GGATGCTGTT 
AAAAATCAAA GGAAGGTACT TTCTGCTCTG GTCAAGCGGC TTGGAGATAA GCATACGGCT 
TTACAGAAAT CTACTAAAGA TGTTCGAACT TCGATTCGAC AGGTGAGTGA TTTACAGAAG 
AGATTGCAAG TGGATGTAAA GATGGCAATC TTGCACATAA TGAAGGAACT TAATAAACGT 
GGAAAACTAC TAGTGAATGA TATGCAGAAA GTAACAGAAG GTCATCAGGA TAAACTGGAA 
AAGCAGCACT GGGCTATGAA CAAATTGCAA AAGCATCAAG AGCACATCCT TAGGTTTGCC 
ACGTGGGCAC TGGAAAGTGA CAACAACATA GCCTTATTGC TTTCAAAGAA ACTGATACAT 
TTTCAGTTGC ATCGTGCTCT AAAAGTTATT GTAGATCCAG TTGAGCCTTT AGGTGATTTA 
AAGTTCCAGT GGGACTCTGA TACCTGGACA AAGCATGCAG AGTTTTTTGG CAAAATTGTA 
TGCGACAAAA CAGGTGTTCC TCAGTCATCT TCTGGATCGT TGGCAGCCCA GTCTATAAAT 
TCTGCGTCAT CTCAGGTGCT GGCTTCTCTT ACTAACTTTA ATCCAATGGT ACAGAATCGA 
CCAAGGCTTG GTGCTTCCCA GATTCAAGCA TCTCCAGCCA GAGCTGGTAC AGCTGGTAAC 
TTCATCCAGC CCATGCAGAT GATGGATGAC TTTGATGGGT CTTCTGCAGA GGGAGAATCT 
TCTGGATTTG ATACTTTCCC TGGCCAGAAA AGAGGGCGCA GTTCTGAAGG TGATGTAAAT 
GAGCTTTTAA AAAAAGTTCC ACGTGTCAGT TTAGAGCGCT TGGATGTAGA CCTTAACCCC 
GACACTCAAC CACCAGTCTT CAAAGTTTTC CCCGGAAACA CAAATGAAGA TTATAATTTA 
ATTGTAATTG AGAGAGGGAA TCTTCCAGGG GAAGCTGTTG TGAAGGAAGA AGCCATGGAA 
ACAGGCATCC CTATCCCAAC ATCTGACCGC TCTGAAAAAT CCTTAGTTAT TCGACCAGAT 
GGCTCGACAG AAACTGTGCC ACCTGTAATC CGCCAGCCTA CTGCAGCTGG TAATGCCACT 
TCCTCTGATT TCCAAGGAGC AAGCAGCGGG GGATTTTCTG ATGGGCTCAG TGGTGCCCGT 
TGTAAAGTGT GCCAGAAGGA AGGAGCCCTA CTGACATGTA GCCAATGTAG AAGCCTTTTC 
CACAGAGACT GCCACCTACC ATCAGTCATG GAAATGACAA GTGGTGCATG GAAGTGCATG 
TTGTGCCAGG ATCTCGTGCC CAAAAACGAT CAAGGCGTCG ACATGATTCA GTCACCAGCT 
GTAGATTCCA GCCGTCTGTC TCCGTCTGAT CAGAAGAAAT GTGAACGTCT GTTGTTGGAA 
ATGCTTTGTC ATGAGCCGTG CCGGCCCTTC CACAGGTTAT CCTCCAGTTC AGAAAATGAT 
CCTATGATTG ACCTTACATT TATTCGTGCC AGACTGCAGC AGAAACGCTC CCCTTCATAT 
AGTACACCTG AAGACTTTGT TAGAGACGTG TGGATGATGT TCAGAAGCGT GAGCAAACTT 
GCAGAGGACA AAGCTGTGGT GCAGTCGATC ATTGAGCTGC AGTCTTTCTT CGAAACAAGA 
CTTAACCACC TTTTTGGTGA CCGCAAGTTC TCCTGTCTCC TGGGGGAGTT TTTAAGTGAG 
GAGGAGCAGC AAAGCGTTAC ACAAAGAAGT TCCCCGATGA GCACAACCAG CGAGAGTGTA 
TCATCTGTCA AAAACACCAC TGAGCAGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950960.1
CDS	350..2602
Translation	MATEAAGSPTGRSYWRAAGCARSSYGRTRSPSSYRACTPCAGPAFRQRPLRLRWPLTAETWLQRCTSCDDNTVANSYCVECTEWLCETCVEAHQRVKYTKDHTIKVKSSSKKETERAVYCPLHKNEPLMLYCESCDTLTCRDCQLQGHKDHQYQFLEDAVKNQRKVLSALVKRLGDKHTALQKSTKDVRTSIRQVSDLQKRLQVDVKMAILHIMKELNKRGKLLVNDMQKVTEGHQDKLEKQHWAMNKLQKHQEHILRFATWALESDNNIALLLSKKLIHFQLHRALKVIVDPVEPLGDLKFQWDSDTWTKHAEFFGKIVCDKTGVPQSSSGSLAAQSINSASSQVLASLTNFNPMVQNRPRLGASQIQASPARAGTAGNFIQPMQMMDDFDGSSAEGESSGFDTFPGQKRGRSSEGDVNELLKKVPRVSLERLDVDLNPDTQPPVFKVFPGNTNEDYNLIVIERGNLPGEAVVKEEAMETGIPIPTSDRSEKSLVIRPDGSTETVPPVIRQPTAAGNATSSDFQGASSGGFSDGLSGARCKVCQKEGALLTCSQCRSLFHRDCHLPSVMEMTSGAWKCMLCQDLVPKNDQGVDMIQSPAVDSSRLSPSDQKKCERLLLEMLCHEPCRPFHRLSSSSENDPMIDLTFIRARLQQKRSPSYSTPEDFVRDVWMMFRSVSKLAEDKAVVQSIIELQSFFETRLNHLFGDRKFSCLLGEFLSEEEQQSVTQRSSPMSTTSESVSSVKNTTEQD

Target ORF information:

RefSeq Version	XM_018095471.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095471.1

ORF Insert Sequence:

ATGGCAACGG AGGCAGCGGG GAGCCCGACG GGCAGGAGCT ACTGGAGAGC TGCGGGGTGT 
GCAAGAAGCA GTTACGGGCG GACACGGAGC CCCAGCTCTT ACCGTGCCTG CACTCCGTGT 
GCCGGGCCTG CCTTCCGCCA GAGACCCCTG CGCCTGAGGT GGCCACTAAC AGCGGAGACT 
TGGCTTCAGA GATGTACCAG CTGTGATGAC AATACTGTAG CAAATAGTTA CTGCGTCGAG 
TGCACAGAGT GGCTATGTGA GACCTGTGTG GAGGCGCATC AGCGAGTGAA ATACACAAAG 
GATCACACCA TCAAAGTTAA AAGTTCTTCC AAAAAGGAAA CAGAGCGAGC AGTTTACTGT 
CCTTTGCATA AAAATGAACC TCTCATGCTT TATTGTGAAT CCTGTGATAC CTTAACTTGC 
CGGGACTGCC AACTACAGGG ACACAAAGAT CACCAGTACC AGTTTTTAGA GGATGCTGTT 
AAAAATCAAA GGAAGGTACT TTCTGCTCTG GTCAAGCGGC TTGGAGATAA GCATACGGCT 
TTACAGAAAT CTACTAAAGA TGTTCGAACT TCGATTCGAC AGGTGAGTGA TTTACAGAAG 
AGATTGCAAG TGGATGTAAA GATGGCAATC TTGCACATAA TGAAGGAACT TAATAAACGT 
GGAAAACTAC TAGTGAATGA TATGCAGAAA GTAACAGAAG GTCATCAGGA TAAACTGGAA 
AAGCAGCACT GGGCTATGAA CAAATTGCAA AAGCATCAAG AGCACATCCT TAGGTTTGCC 
ACGTGGGCAC TGGAAAGTGA CAACAACATA GCCTTATTGC TTTCAAAGAA ACTGATACAT 
TTTCAGTTGC ATCGTGCTCT AAAAGTTATT GTAGATCCAG TTGAGCCTTT AGGTGATTTA 
AAGTTCCAGT GGGACTCTGA TACCTGGACA AAGCATGCAG AGTTTTTTGG CAAAATTGTA 
TGCGACAAAA CAGGTGTTCC TCAGTCATCT TCTGGATCGT TGGCAGCCCA GTCTATAAAT 
TCTGCGTCAT CTCAGGTGCT GGCTTCTCTT ACTAACTTTA ATCCAATGGT ACAGAATCGA 
CCAAGGCTTG GTGCTTCCCA GATTCAAGCA TCTCCAGCCA GAGCTGGTAC AGCTGGTAAC 
TTCATCCAGC CCATGCAGAT GATGGATGAC TTTGATGGGT CTTCTGCAGA GGGAGAATCT 
TCTGGATTTG ATACTTTCCC TGGCCAGAAA AGAGGGCGCA GTTCTGAAGG TGATGTAAAT 
GAGCTTTTAA AAAAAGTTCC ACGTGTCAGT TTAGAGCGCT TGGATGTAGA CCTTAACCCC 
GACACTCAAC CACCAGTCTT CAAAGTTTTC CCCGGAAACA CAAATGAAGA TTATAATTTA 
ATTGTAATTG AGAGAGGGAA TCTTCCAGGG GAAGCTGTTG TGAAGGAAGA AGCCATGGAA 
ACAGGCATCC CTATCCCAAC ATCTGACCGC TCTGAAAAAT CCTTAGTTAT TCGACCAGAT 
GGCTCGACAG AAACTGTGCC ACCTGTAATC CGCCAGCCTA CTGCAGCTGG TAATGCCACT 
TCCTCTGATT TCCAAGGAGC AAGCAGCGGG GGATTTTCTG ATGGGCTCAG TGGTGCCCGT 
TGTAAAGTGT GCCAGAAGGA AGGAGCCCTA CTGACATGTA GCCAATGTAG AAGCCTTTTC 
CACAGAGACT GCCACCTACC ATCAGTCATG GAAATGACAA GTGGTGCATG GAAGTGCATG 
TTGTGCCAGG ATCTCGTGCC CAAAAACGAT CAAGGCGTCG ACATGATTCA GTCACCAGCT 
GTAGATTCCA GCCGTCTGTC TCCGTCTGAT CAGAAGAAAT GTGAACGTCT GTTGTTGGAA 
ATGCTTTGTC ATGAGCCGTG CCGGCCCTTC CACAGGTTAT CCTCCAGTTC AGAAAATGAT 
CCTATGATTG ACCTTACATT TATTCGTGCC AGACTGCAGC AGAAACGCTC CCCTTCATAT 
AGTACACCTG AAGACTTTGT TAGAGACGTG TGGATGATGT TCAGAAGCGT GAGCAAACTT 
GCAGAGGACA AAGCTGTGGT GCAGTCGATC ATTGAGCTGC AGTCTTTCTT CGAAACAAGA 
CTTAACCACC TTTTTGGTGA CCGCAAGTTC TCCTGTCTCC TGGGGGAGTT TTTAAGTGAG 
GAGGAGCAGC AAAGCGTTAC ACAAAGAAGT TCCCCGATGA GCACAACCAG CGAGAGTGTA 
TCATCTGTCA AAAACACCAC TGAGCAGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim28 ( XM_031905703.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761563.1 Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X2, mRNA.

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CloneID	OXa62118
Clone ID Related Accession (Same CDS sequence)	XM_031905703.1
Accession Version	XM_031905703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcription intermediary factor 1-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATGTA CCAGCTGTGA TGACAATACT GTAGCAAATA GTTACTGCGT CGAGTGCACA 
GAGTGGCTAT GTGAGACCTG TGTGGAGGCG CATCAGCGAG TGAAATACAC AAAGGATCAC 
ACCATCAAAG TTAAAAGTTC TTCCAAAAAG GAAACAGAGC GAGCAGTTTA CTGTCCTTTG 
CATAAAAATG AACCTCTCAT GCTTTATTGT GAATCCTGTG ATACCTTAAC TTGCCGGGAC 
TGCCAACTAC AGGGACACAA AGATCACCAG TACCAGTTTT TAGAGGATGC TGTTAAAAAT 
CAAAGGAAGG TACTTTCTGC TCTGGTCAAG CGGCTTGGAG ATAAGCATAC GGCTTTACAG 
AAATCTACTA AAGATGTTCG AACTTCGATT CGACAGGTGA GTGATTTACA GAAGAGATTG 
CAAGTGGATG TAAAGATGGC AATCTTGCAC ATAATGAAGG AACTTAATAA ACGTGGAAAA 
CTACTAGTGA ATGATATGCA GAAAGTAACA GAAGGTCATC AGGATAAACT GGAAAAGCAG 
CACTGGGCTA TGAACAAATT GCAAAAGCAT CAAGAGCACA TCCTTAGGTT TGCCACGTGG 
GCACTGGAAA GTGACAACAA CATAGCCTTA TTGCTTTCAA AGAAACTGAT ACATTTTCAG 
TTGCATCGTG CTCTAAAAGT TATTGTAGAT CCAGTTGAGC CTTTAGGTGA TTTAAAGTTC 
CAGTGGGACT CTGATACCTG GACAAAGCAT GCAGAGTTTT TTGGCAAAAT TGTATGCGAC 
AAAACAGGTG TTCCTCAGTC ATCTTCTGGA TCGTTGGCAG CCCAGTCTAT AAATTCTGCG 
TCATCTCAGA TGATGGATGA CTTTGATGGG TCTTCTGCAG AGGGAGAATC TTCTGGATTT 
GATACTTTCC CTGGCCAGAA AAGAGGGCGC AGTTCTGAAG GTGATGTAAA TGAGCTTTTA 
AAAAAAGTTC CACGTGTCAG TTTAGAGCGC TTGGATGTAG ACCTTAACCC CGACACTCAA 
CCACCAGTCT TCAAAGTTTT CCCCGGAAAC ACAAATGAAG ATTATAATTT AATTGTAATT 
GAGAGAGGGA ATCTTCCAGG GGAAGCTGTT GTGAAGGAAG AAGCCATGGA AACAGGCATC 
CCTATCCCAA CATCTGACCG CTCTGAAAAA TCCTTAGTTA TTCGACCAGA TGGCTCGACA 
GAAACTGTGC CACCTGTAAT CCGCCAGCCT ACTGCAGCTG GTAATGCCAC TTCCTCTGAT 
TTCCAAGGAG CAAGCAGCGG GGGATTTTCT GATGGGCTCA GTGGTGCCCG TTGTAAAGTG 
TGCCAGAAGG AAGGAGCCCT ACTGACATGT AGCCAATGTA GAAGCCTTTT CCACAGAGAC 
TGCCACCTAC CATCAGTCAT GGAAATGACA AGTGGTGCAT GGAAGTGCAT GTTGTGCCAG 
GATCTCGTGC CCAAAAACGA TCAAGGCGTC GACATGATTC AGTCACCAGC TGTAGATTCC 
AGCCGTCTGT CTCCGTCTGA TCAGAAGAAA TGTGAACGTC TGTTGTTGGA AATGCTTTGT 
CATGAGCCGT GCCGGCCCTT CCACAGGTTA TCCTCCAGTT CAGAAAATGA TCCTATGATT 
GACCTTACAT TTATTCGTGC CAGACTGCAG CAGAAACGCT CCCCTTCATA TAGTACACCT 
GAAGACTTTG TTAGAGACGT GTGGATGATG TTCAGAAGCG TGAGCAAACT TGCAGAGGAC 
AAAGCTGTGG TGCAGTCGAT CATTGAGCTG CAGTCTTTCT TCGAAACAAG ACTTAACCAC 
CTTTTTGGTG ACCGCAAGTT CTCCTGTCTC CTGGGGGAGT TTTTAAGTGA GGAGGAGCAG 
CAAAGCGTTA CACAAAGAAG TTCCCCGATG AGCACAACCA GCGAGAGTGT ATCATCTGTC 
AAAAACACCA CTGAGCAGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761563.1
CDS	284..2227
Translation	MRCTSCDDNTVANSYCVECTEWLCETCVEAHQRVKYTKDHTIKVKSSSKKETERAVYCPLHKNEPLMLYCESCDTLTCRDCQLQGHKDHQYQFLEDAVKNQRKVLSALVKRLGDKHTALQKSTKDVRTSIRQVSDLQKRLQVDVKMAILHIMKELNKRGKLLVNDMQKVTEGHQDKLEKQHWAMNKLQKHQEHILRFATWALESDNNIALLLSKKLIHFQLHRALKVIVDPVEPLGDLKFQWDSDTWTKHAEFFGKIVCDKTGVPQSSSGSLAAQSINSASSQMMDDFDGSSAEGESSGFDTFPGQKRGRSSEGDVNELLKKVPRVSLERLDVDLNPDTQPPVFKVFPGNTNEDYNLIVIERGNLPGEAVVKEEAMETGIPIPTSDRSEKSLVIRPDGSTETVPPVIRQPTAAGNATSSDFQGASSGGFSDGLSGARCKVCQKEGALLTCSQCRSLFHRDCHLPSVMEMTSGAWKCMLCQDLVPKNDQGVDMIQSPAVDSSRLSPSDQKKCERLLLEMLCHEPCRPFHRLSSSSENDPMIDLTFIRARLQQKRSPSYSTPEDFVRDVWMMFRSVSKLAEDKAVVQSIIELQSFFETRLNHLFGDRKFSCLLGEFLSEEEQQSVTQRSSPMSTTSESVSSVKNTTEQD

Target ORF information:

RefSeq Version	XM_031905703.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 28 (trim28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905703.1

ORF Insert Sequence:

ATGAGATGTA CCAGCTGTGA TGACAATACT GTAGCAAATA GTTACTGCGT CGAGTGCACA 
GAGTGGCTAT GTGAGACCTG TGTGGAGGCG CATCAGCGAG TGAAATACAC AAAGGATCAC 
ACCATCAAAG TTAAAAGTTC TTCCAAAAAG GAAACAGAGC GAGCAGTTTA CTGTCCTTTG 
CATAAAAATG AACCTCTCAT GCTTTATTGT GAATCCTGTG ATACCTTAAC TTGCCGGGAC 
TGCCAACTAC AGGGACACAA AGATCACCAG TACCAGTTTT TAGAGGATGC TGTTAAAAAT 
CAAAGGAAGG TACTTTCTGC TCTGGTCAAG CGGCTTGGAG ATAAGCATAC GGCTTTACAG 
AAATCTACTA AAGATGTTCG AACTTCGATT CGACAGGTGA GTGATTTACA GAAGAGATTG 
CAAGTGGATG TAAAGATGGC AATCTTGCAC ATAATGAAGG AACTTAATAA ACGTGGAAAA 
CTACTAGTGA ATGATATGCA GAAAGTAACA GAAGGTCATC AGGATAAACT GGAAAAGCAG 
CACTGGGCTA TGAACAAATT GCAAAAGCAT CAAGAGCACA TCCTTAGGTT TGCCACGTGG 
GCACTGGAAA GTGACAACAA CATAGCCTTA TTGCTTTCAA AGAAACTGAT ACATTTTCAG 
TTGCATCGTG CTCTAAAAGT TATTGTAGAT CCAGTTGAGC CTTTAGGTGA TTTAAAGTTC 
CAGTGGGACT CTGATACCTG GACAAAGCAT GCAGAGTTTT TTGGCAAAAT TGTATGCGAC 
AAAACAGGTG TTCCTCAGTC ATCTTCTGGA TCGTTGGCAG CCCAGTCTAT AAATTCTGCG 
TCATCTCAGA TGATGGATGA CTTTGATGGG TCTTCTGCAG AGGGAGAATC TTCTGGATTT 
GATACTTTCC CTGGCCAGAA AAGAGGGCGC AGTTCTGAAG GTGATGTAAA TGAGCTTTTA 
AAAAAAGTTC CACGTGTCAG TTTAGAGCGC TTGGATGTAG ACCTTAACCC CGACACTCAA 
CCACCAGTCT TCAAAGTTTT CCCCGGAAAC ACAAATGAAG ATTATAATTT AATTGTAATT 
GAGAGAGGGA ATCTTCCAGG GGAAGCTGTT GTGAAGGAAG AAGCCATGGA AACAGGCATC 
CCTATCCCAA CATCTGACCG CTCTGAAAAA TCCTTAGTTA TTCGACCAGA TGGCTCGACA 
GAAACTGTGC CACCTGTAAT CCGCCAGCCT ACTGCAGCTG GTAATGCCAC TTCCTCTGAT 
TTCCAAGGAG CAAGCAGCGG GGGATTTTCT GATGGGCTCA GTGGTGCCCG TTGTAAAGTG 
TGCCAGAAGG AAGGAGCCCT ACTGACATGT AGCCAATGTA GAAGCCTTTT CCACAGAGAC 
TGCCACCTAC CATCAGTCAT GGAAATGACA AGTGGTGCAT GGAAGTGCAT GTTGTGCCAG 
GATCTCGTGC CCAAAAACGA TCAAGGCGTC GACATGATTC AGTCACCAGC TGTAGATTCC 
AGCCGTCTGT CTCCGTCTGA TCAGAAGAAA TGTGAACGTC TGTTGTTGGA AATGCTTTGT 
CATGAGCCGT GCCGGCCCTT CCACAGGTTA TCCTCCAGTT CAGAAAATGA TCCTATGATT 
GACCTTACAT TTATTCGTGC CAGACTGCAG CAGAAACGCT CCCCTTCATA TAGTACACCT 
GAAGACTTTG TTAGAGACGT GTGGATGATG TTCAGAAGCG TGAGCAAACT TGCAGAGGAC 
AAAGCTGTGG TGCAGTCGAT CATTGAGCTG CAGTCTTTCT TCGAAACAAG ACTTAACCAC 
CTTTTTGGTG ACCGCAAGTT CTCCTGTCTC CTGGGGGAGT TTTTAAGTGA GGAGGAGCAG 
CAAAGCGTTA CACAAAGAAG TTCCCCGATG AGCACAACCA GCGAGAGTGT ATCATCTGTC 
AAAAACACCA CTGAGCAGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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trim28 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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