kdm6a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	kdm6a
Entrez Gene ID	100170558
Full Name	lysine demethylase 6A
Synonyms	tutx,utx,xtutx
General protein information	Preferred Names lysine demethylase 6A Names lysine-specific demethylase 6A lysine (K)-specific demethylase 6A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012956544.2	XP_012811998.1	lysine-specific demethylase 6A isoform X2
XM_012956542.2	XP_012811996.1	lysine-specific demethylase 6A isoform X1
XM_012956544.3	XP_012811998.1	lysine-specific demethylase 6A isoform X2
NM_001130335.1	NP_001123807.1	lysine-specific demethylase 6A
XM_031895734.1	XP_031751594.1	lysine-specific demethylase 6A isoform X3
XM_012956542.3	XP_012811996.1	lysine-specific demethylase 6A isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008509	XP_316935.4
Homo sapiens (human)	KDM6A	NP_066963.2
Danio rerio (zebrafish)	kdm6a	XP_697746.2
Xenopus tropicalis (tropical clawed frog)	kdm6a	NP_001123807.1
Bos taurus (cattle)	KDM6A	NP_001193504.1
Drosophila melanogaster (fruit fly)	Utx	NP_001188773.1
Pan troglodytes (chimpanzee)	KDM6A	XP_003954022.1
Canis lupus familiaris (dog)	KDM6A	NP_001184114.1
Caenorhabditis elegans (roundworm)	utx-1	NP_509450.1
Rattus norvegicus (Norway rat)	Kdm6a	XP_006256701.1
Mus musculus (house mouse)	Kdm6a	NP_033509.1
Gallus gallus (chicken)	KDM6A	XP_416762.4
Macaca mulatta (Rhesus monkey)	KDM6A	XP_002806253.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparative expression analysis of the H3K27 demethylases, JMJD3 and UTX, with the H3K27 methylase, EZH2, in Xenopus.
Kawaguchi A, Ochi H, Sudou N, Ogino H
The International journal of developmental biology56(4)295-300(2012)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following kdm6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa23259	NM_001130335.1 Latest version!	Xenopus tropicalis lysine demethylase 6A (kdm6a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32428	XM_012956542.3 Latest version!	Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32429	XM_012956544.3 Latest version!	Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32430	XM_031895734.1 Latest version!	Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OXa32429	XM_012956544.2	Xenopus tropicalis lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32428	XM_012956542.2	Xenopus tropicalis lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kdm6a ( NM_001130335.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123807.1 Xenopus tropicalis lysine demethylase 6A (kdm6a), mRNA.

Price & Availability↑

CloneID	OXa23259
Clone ID Related Accession (Same CDS sequence)	NM_001130335.1
Accession Version	NM_001130335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167610.1. ##Evidence-Data-START## Transcript exon combination :: BC167610.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT GCTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACTCAGCAC AGCAGAATAC TTCTGATACC TGGAGCAGTG GCCATTTGGC GTCACATACT 
CCAGGACAGC AACAAGTACA TTCGTGGTGT TTGCCGCCAC ACAAAGTACA GCAATTGGAA 
CAGCTACGAG CAAACAGGAA TAATCTGAAT CCAGCACAAA AAGCGGCGTT GGAGCAGTTG 
GAGGCTCAGT TTGTCTTAAT GCAGCAGCAA CAGCAGATGA GACAAACTGG ACTTTCACAG 
GTACGTTCTA CTGGAATTGC TAACGGGCCA ACTGCTGACT CAACACTGCC TACAAACTCA 
CTCACTGGTC AGCAACATCA GGTTGCATTA ACCAGGATGC CTAATGCCGC TCAGCCTGGA 
ATCCGTCCGG CTTGCCCTGC GCAGCCTGTG GCTAATGGAC CATTTCCAGT AGGCCAAGTT 
TCCTGCAGTA CCACAGGGCC AGTGGGCAGT GCAGACACTA TGTTGATAGG CAATAATCAT 
ATTACGGGAA ATGGAAGTAA TGGAAATGTG CCTTACCCGC AGCGAAACGC ACTCGCACTA 
CCTCATAACC GCACAAACCT GACCAGCAGC GCAGAGGAGC CGTGGAAAAA CCAACTAACT 
AACTCCACTC AGGGGCTTCA TAAAGGTCAG AGTTCACATT TGGCAGGTCC TAATTGTGAA 
CGACCTCCTT CTTCCACTGG GCCTTCCCAG CATCTCCAAG CAGCTGGCAC TGGTATACAA 
AATCAGGTTG GACATCCTAC CATGCCTAGC AATTCAGTAA CACAGGGGGC TGCTCTCAAT 
CACCTCTCCT CTCACACTGC TACCTCAGGT GGACAACAAG GCATTATTTT AACCAAAGAG 
AGCAAGCCTT CAGGAAATGG ATCAGTGCCT GAAACAAGCA GGCATTCTGA GGGGACACCT 
AACAGCACTG CCGGTGTAGA GGGACTTCCT AATCATGTCC ATCAGGTGAC GGCAGATACT 
GTTCCTAGCC CTAGCCATGG AGATTCAAAG TCACCAGGTT TACTTAGTTC AGACAATCCT 
CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC AATAACAATG TAGGTCCTGG AACCTGTGAC 
AAGGTCAACA ATGTTCATCC AGCTGTTCAT ACAAAAACTG AGAATTCTGT TGCCTCCTCT 
CCATCTTCAG CCATTTCCAC AGCAACACCT TCTCCAAAAT CTACCGAACA GACTATGACC 
AACAGTGTTG TCAGCCTTAA AAGCCCTCTC AGTGGACATG ATAAAGTGAA TGGAGAGCAA 
ACGGAGGACT CTCAGAGCCC CACAAAAAAG GACCACTCTT TACCTAGCCA CAAATTAAGT 
CCACAGACCA TACCATCCAT GTCTGTATCC ATTTACCCTA GCTCAGTAGA GGTTTTAAAG 
GCTTGTCGAA ATCTGGGTAG AAATGGTTTA TCGAACAGTA GTATTTTACT GGACAAGTGC 
CCACCACCAA GGCCGCCGCC TCCACCCTAT CCACCTTTAC CAACAGATAA ATTAAATCCT 
CCAACACCGA GCATTTACTT AGAAAACAAG AGAGATGCTT TCTTCCCACC ACTCCATCAG 
TTCTGCACAA ATCCAAACAA CCCTGTTACT GTCATACGAG GCCTTGCTGG AGCTCTTAAA 
TTAGATCTAG GACTATTTTC TACAAAAACA CTAGTAGAAG CGAATAATGA ACATATAGTT 
GAAGTGAGGA CACAGTTATT ACAGCCTGCA GACGAAAACT GGGACCCCAC TGGAACAAAG 
AAAATCTGGC GCTGTGAAAG CAGTCGTTCT CATACCACCA TTGCAAAATA TGCACAGTAT 
CAGGCCGCTT CATTCCAAGA ATCATTAAGA GAGGAAAATG AGAAGAAAAG CCAACACAAA 
GACCATTCAG ATAGTGAATC CACATCTTCA GATAACTCTG GTAGAAGACG TAAAGGCCCT 
TTTAAAACCA TTAAATTTGG GACCAATATT GATTTGTCTG ATGAAAGAAA GTGGAAACTA 
CAGCTTCATG AACTGACCAA ACTTCCTGCG TTTGCACGTG TTGTGTCAGC TGGAAATCTT 
CTGAGTCATG TTGGCCATAC TATACTAGGA ATGAACACCG TTCAACTCTA CATGAAGGTT 
CCAGGCAGTA GGACACCAGG CCATCAAGAG AACAACAACT TCTGTTCGGT TAATATTAAT 
ATTGGTCCGG GAGATTGTGA ATGGTTTGTG GTACCTGAGA CGTACTGGAG CGTAATGCAT 
GACTTCTGTG AAAAGAATAA TATAAACTTC CTTTTGGGAT CATGGTGGCC CAATCTTGAA 
GACCTATATG AAGCAAATGT GCCAGTCTAC AGGTTTATTC AAAGGCCTGG CGACTTAGTT 
TGGATTAATG CTGGCACAGT GCATTGGGTT CAAGCAATTG GTTGGTGTAA CAACATTGCA 
TGGAATGTTG GCCCTCTCAC AGCCTTCCAG TATAAACTGG CAGTGGAACG GTACGAATGG 
AATAAATTGC AGTGCGTCAA GTCTATTGTA CCAATGGTTC ATCTCTCTTG GAATATGGCA 
AAGAATATCA AAGTTTCAGA TCCAAAGCTT TTTGAAATGA TTAAATATTG CTTATTGAGG 
ACCTTGAAGC ACTGTCAAGC ACTGAGAGAA GTTCTTAATG CTTCTGGAAT TGAAATTGTG 
TGGCATGGCA GAACGAAAGA TGAGCCTGCT CATTACTGTA GCAACTGTGA GGTGGAAGTC 
TTCAATTTAC TTTTTGTGAC AAAAGAAAGC AATTCTCAGA AGACCTATGT TGTTCATTGC 
CATGACTGCT CACGAAAATC ATGCCCAAAC CTAGAAAATT TTGTGGTGCT AGAACAGTTC 
AAAATGGAGG AGCTGATGCA GATCTATGAC CTATTTACAT TAGCTCCTCC AATGCCTTCA 
CCATCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123807.1
CDS	603..4814
Translation	MKSCGVSLSAAAVVAAAAASLGDEKKKMAAGKASETEEDFPNLTAEEREALVGLDSRLYGFLRLHEDGARTKALLLKAIRCYEALILKSEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFHWAIRAFQEVLYVEPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDCNPCTLSKAEIQFHIAHLYETQKKYHSAKEAYEQLLQTENLPTQVKATVLQQLGWMHHTVDQLGDKATKASYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCNNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNSAQQNTSDTWSSGHLASHTPGQQQVHSWCLPPHKVQQLEQLRANRNNLNPAQKAALEQLEAQFVLMQQQQQMRQTGLSQVRSTGIANGPTADSTLPTNSLTGQQHQVALTRMPNAAQPGIRPACPAQPVANGPFPVGQVSCSTTGPVGSADTMLIGNNHITGNGSNGNVPYPQRNALALPHNRTNLTSSAEEPWKNQLTNSTQGLHKGQSSHLAGPNCERPPSSTGPSQHLQAAGTGIQNQVGHPTMPSNSVTQGAALNHLSSHTATSGGQQGIILTKESKPSGNGSVPETSRHSEGTPNSTAGVEGLPNHVHQVTADTVPSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNVHPAVHTKTENSVASSPSSAISTATPSPKSTEQTMTNSVVSLKSPLSGHDKVNGEQTEDSQSPTKKDHSLPSHKLSPQTIPSMSVSIYPSSVEVLKACRNLGRNGLSNSSILLDKCPPPRPPPPPYPPLPTDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHIVEVRTQLLQPADENWDPTGTKKIWRCESSRSHTTIAKYAQYQAASFQESLREENEKKSQHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDERKWKLQLHELTKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPETYWSVMHDFCEKNNINFLLGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTAFQYKLAVERYEWNKLQCVKSIVPMVHLSWNMAKNIKVSDPKLFEMIKYCLLRTLKHCQALREVLNASGIEIVWHGRTKDEPAHYCSNCEVEVFNLLFVTKESNSQKTYVVHCHDCSRKSCPNLENFVVLEQFKMEELMQIYDLFTLAPPMPSPSS

Target ORF information:

RefSeq Version	NM_001130335.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine demethylase 6A (kdm6a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130335.1

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT GCTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACTCAGCAC AGCAGAATAC TTCTGATACC TGGAGCAGTG GCCATTTGGC GTCACATACT 
CCAGGACAGC AACAAGTACA TTCGTGGTGT TTGCCGCCAC ACAAAGTACA GCAATTGGAA 
CAGCTACGAG CAAACAGGAA TAATCTGAAT CCAGCACAAA AAGCGGCGTT GGAGCAGTTG 
GAGGCTCAGT TTGTCTTAAT GCAGCAGCAA CAGCAGATGA GACAAACTGG ACTTTCACAG 
GTACGTTCTA CTGGAATTGC TAACGGGCCA ACTGCTGACT CAACACTGCC TACAAACTCA 
CTCACTGGTC AGCAACATCA GGTTGCATTA ACCAGGATGC CTAATGCCGC TCAGCCTGGA 
ATCCGTCCGG CTTGCCCTGC GCAGCCTGTG GCTAATGGAC CATTTCCAGT AGGCCAAGTT 
TCCTGCAGTA CCACAGGGCC AGTGGGCAGT GCAGACACTA TGTTGATAGG CAATAATCAT 
ATTACGGGAA ATGGAAGTAA TGGAAATGTG CCTTACCCGC AGCGAAACGC ACTCGCACTA 
CCTCATAACC GCACAAACCT GACCAGCAGC GCAGAGGAGC CGTGGAAAAA CCAACTAACT 
AACTCCACTC AGGGGCTTCA TAAAGGTCAG AGTTCACATT TGGCAGGTCC TAATTGTGAA 
CGACCTCCTT CTTCCACTGG GCCTTCCCAG CATCTCCAAG CAGCTGGCAC TGGTATACAA 
AATCAGGTTG GACATCCTAC CATGCCTAGC AATTCAGTAA CACAGGGGGC TGCTCTCAAT 
CACCTCTCCT CTCACACTGC TACCTCAGGT GGACAACAAG GCATTATTTT AACCAAAGAG 
AGCAAGCCTT CAGGAAATGG ATCAGTGCCT GAAACAAGCA GGCATTCTGA GGGGACACCT 
AACAGCACTG CCGGTGTAGA GGGACTTCCT AATCATGTCC ATCAGGTGAC GGCAGATACT 
GTTCCTAGCC CTAGCCATGG AGATTCAAAG TCACCAGGTT TACTTAGTTC AGACAATCCT 
CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC AATAACAATG TAGGTCCTGG AACCTGTGAC 
AAGGTCAACA ATGTTCATCC AGCTGTTCAT ACAAAAACTG AGAATTCTGT TGCCTCCTCT 
CCATCTTCAG CCATTTCCAC AGCAACACCT TCTCCAAAAT CTACCGAACA GACTATGACC 
AACAGTGTTG TCAGCCTTAA AAGCCCTCTC AGTGGACATG ATAAAGTGAA TGGAGAGCAA 
ACGGAGGACT CTCAGAGCCC CACAAAAAAG GACCACTCTT TACCTAGCCA CAAATTAAGT 
CCACAGACCA TACCATCCAT GTCTGTATCC ATTTACCCTA GCTCAGTAGA GGTTTTAAAG 
GCTTGTCGAA ATCTGGGTAG AAATGGTTTA TCGAACAGTA GTATTTTACT GGACAAGTGC 
CCACCACCAA GGCCGCCGCC TCCACCCTAT CCACCTTTAC CAACAGATAA ATTAAATCCT 
CCAACACCGA GCATTTACTT AGAAAACAAG AGAGATGCTT TCTTCCCACC ACTCCATCAG 
TTCTGCACAA ATCCAAACAA CCCTGTTACT GTCATACGAG GCCTTGCTGG AGCTCTTAAA 
TTAGATCTAG GACTATTTTC TACAAAAACA CTAGTAGAAG CGAATAATGA ACATATAGTT 
GAAGTGAGGA CACAGTTATT ACAGCCTGCA GACGAAAACT GGGACCCCAC TGGAACAAAG 
AAAATCTGGC GCTGTGAAAG CAGTCGTTCT CATACCACCA TTGCAAAATA TGCACAGTAT 
CAGGCCGCTT CATTCCAAGA ATCATTAAGA GAGGAAAATG AGAAGAAAAG CCAACACAAA 
GACCATTCAG ATAGTGAATC CACATCTTCA GATAACTCTG GTAGAAGACG TAAAGGCCCT 
TTTAAAACCA TTAAATTTGG GACCAATATT GATTTGTCTG ATGAAAGAAA GTGGAAACTA 
CAGCTTCATG AACTGACCAA ACTTCCTGCG TTTGCACGTG TTGTGTCAGC TGGAAATCTT 
CTGAGTCATG TTGGCCATAC TATACTAGGA ATGAACACCG TTCAACTCTA CATGAAGGTT 
CCAGGCAGTA GGACACCAGG CCATCAAGAG AACAACAACT TCTGTTCGGT TAATATTAAT 
ATTGGTCCGG GAGATTGTGA ATGGTTTGTG GTACCTGAGA CGTACTGGAG CGTAATGCAT 
GACTTCTGTG AAAAGAATAA TATAAACTTC CTTTTGGGAT CATGGTGGCC CAATCTTGAA 
GACCTATATG AAGCAAATGT GCCAGTCTAC AGGTTTATTC AAAGGCCTGG CGACTTAGTT 
TGGATTAATG CTGGCACAGT GCATTGGGTT CAAGCAATTG GTTGGTGTAA CAACATTGCA 
TGGAATGTTG GCCCTCTCAC AGCCTTCCAG TATAAACTGG CAGTGGAACG GTACGAATGG 
AATAAATTGC AGTGCGTCAA GTCTATTGTA CCAATGGTTC ATCTCTCTTG GAATATGGCA 
AAGAATATCA AAGTTTCAGA TCCAAAGCTT TTTGAAATGA TTAAATATTG CTTATTGAGG 
ACCTTGAAGC ACTGTCAAGC ACTGAGAGAA GTTCTTAATG CTTCTGGAAT TGAAATTGTG 
TGGCATGGCA GAACGAAAGA TGAGCCTGCT CATTACTGTA GCAACTGTGA GGTGGAAGTC 
TTCAATTTAC TTTTTGTGAC AAAAGAAAGC AATTCTCAGA AGACCTATGT TGTTCATTGC 
CATGACTGCT CACGAAAATC ATGCCCAAAC CTAGAAAATT TTGTGGTGCT AGAACAGTTC 
AAAATGGAGG AGCTGATGCA GATCTATGAC CTATTTACAT TAGCTCCTCC AATGCCTTCA 
CCATCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_012956542.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811996.1 Xenopus tropicalis lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32428
Clone ID Related Accession (Same CDS sequence)	XM_012956542.2 , XM_012956542.3
Accession Version	XM_012956542.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT GCTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACTCAGCAC AGCAGGCCTG TAAACCTCAT CATCAGAATG CTGAATCTGT ACTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCATTACACA TGATTCCTTC TAGCCAGGTA 
GATGAACTGT CAAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAGCAAA GAATACTTCT 
GATACCTGGA GCAGTGGCCA TTTGGCGTCA CATACTCCAG GACAGCAACA AGTACATTCG 
TGGTGTTTGC CGCCACACAA AGTACAGCAA TTGGAACAGC TACGAGCAAA CAGGAATAAT 
CTGAATCCAG CACAAAAAGC GGCGTTGGAG CAGTTGGAGG CTCAGTTTGT CTTAATGCAG 
CAGCAACAGC AGATGAGACA AACTGGACTT TCACAGGTAC GTTCTACTGG AATTGCTAAC 
GGGCCAACTG CTGACTCAAC ACTGCCTACA AACTCACTCA CTGGTCAGCA ACATCAGGTT 
GCATTAACCA GGATGCCTAA TGCCGCTCAG CCTGGAATCC GTCCGGCTTG CCCTGCGCAG 
CCTGTGGCTA ATGGACCATT TCCAGTAGGC CAAGTTTCCT GCAGTACCAC AGGGCCAGTG 
GGCAGTGCAG ACACTATGTT GATAGGCAAT AATCATATTA CGGGAAATGG AAGTAATGGA 
AATGTGCCTT ACCCGCAGCG AAACGCACTC GCACTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTAACTAACT CCACTCAGGG GCTTCATAAA 
GGTCAGAGTT CACATTTGGC AGGTCCTAAT TGTGAACGAC CTCCTTCTTC CACTGGGCCT 
TCCCAGCATC TCCAAGCAGC TGGCACTGGT ATACAAAATC AGGTTGGACA TCCTACCATG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TATTTTAACC AAAGAGAGCA AGCCTTCAGG AAATGGATCA 
GTGCCTGAAA CAAGCAGGCA TTCTGAGGGG ACACCTAACA GCACTGCCGG TGTAGAGGGA 
CTTCCTAATC ATGTCCATCA GGTGACGGCA GATACTGTTC CTAGCCCTAG CCATGGAGAT 
TCAAAGTCAC CAGGTTTACT TAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA 
AAAGCCAATA ACAATGTAGG TCCTGGAACC TGTGACAAGG TCAACAATGT TCATCCAGCT 
GTTCATACAA AAACTGAGAA TTCTGTTGCC TCCTCTCCAT CTTCAGCCAT TTCCACAGCA 
ACACCTTCTC CAAAATCTAC CGAACAGACT ATGACCAACA GTGTTGTCAG CCTTAAAAGC 
CCTCTCAGTG GACATGATAA AGTGAATGGA GAGCAAACGG AGGACTCTCA GAGCCCCACA 
AAAAAGGACC ACTCTTTACC TAGCCACAAA TTAAGTCCAC AGACCATACC ATCCATGTCT 
GTATCCATTT ACCCTAGCTC AGTAGAGGTT TTAAAGGCTT GTCGAAATCT GGGTAGAAAT 
GGTTTATCGA ACAGTAGTAT TTTACTGGAC AAGTGCCCAC CACCAAGGCC GCCGCCTCCA 
CCCTATCCAC CTTTACCAAC AGATAAATTA AATCCTCCAA CACCGAGCAT TTACTTAGAA 
AACAAGAGAG ATGCTTTCTT CCCACCACTC CATCAGTTCT GCACAAATCC AAACAACCCT 
GTTACTGTCA TACGAGGCCT TGCTGGAGCT CTTAAATTAG ATCTAGGACT ATTTTCTACA 
AAAACACTAG TAGAAGCGAA TAATGAACAT ATAGTTGAAG TGAGGACACA GTTATTACAG 
CCTGCAGACG AAAACTGGGA CCCCACTGGA ACAAAGAAAA TCTGGCGCTG TGAAAGCAGT 
CGTTCTCATA CCACCATTGC AAAATATGCA CAGTATCAGG CCGCTTCATT CCAAGAATCA 
TTAAGAGAGG AAAATGAGAA GAAAAGCCAA CACAAAGACC ATTCAGATAG TGAATCCACA 
TCTTCAGATA ACTCTGGTAG AAGACGTAAA GGCCCTTTTA AAACCATTAA ATTTGGGACC 
AATATTGATT TGTCTGATGA AAGAAAGTGG AAACTACAGC TTCATGAACT GACCAAACTT 
CCTGCGTTTG CACGTGTTGT GTCAGCTGGA AATCTTCTGA GTCATGTTGG CCATACTATA 
CTAGGAATGA ACACCGTTCA ACTCTACATG AAGGTTCCAG GCAGTAGGAC ACCAGGCCAT 
CAAGAGAACA ACAACTTCTG TTCGGTTAAT ATTAATATTG GTCCGGGAGA TTGTGAATGG 
TTTGTGGTAC CTGAGACGTA CTGGAGCGTA ATGCATGACT TCTGTGAAAA GAATAATATA 
AACTTCCTTT TGGGATCATG GTGGCCCAAT CTTGAAGACC TATATGAAGC AAATGTGCCA 
GTCTACAGGT TTATTCAAAG GCCTGGCGAC TTAGTTTGGA TTAATGCTGG CACAGTGCAT 
TGGGTTCAAG CAATTGGTTG GTGTAACAAC ATTGCATGGA ATGTTGGCCC TCTCACAGCC 
TTCCAGTATA AACTGGCAGT GGAACGGTAC GAATGGAATA AATTGCAGTG CGTCAAGTCT 
ATTGTGCCAA TGGTTCATCT CTCTTGGAAT ATGGCAAAGA ATATCAAAGT TTCAGATCCA 
AAGCTTTTTG AAATGATTAA ATATTGCTTA TTGAGGACCT TGAAGCACTG TCAAGCACTG 
AGAGAAGTTC TTAATGCTTC TGGAATTGAA ATTGTGTGGC ATGGCAGAAC GAAAGATGAG 
CCTGCTCATT ACTGTAGCAA CTGTGAGGTG GAAGTCTTCA ATTTACTTTT TGTGACAAAA 
GAAAGCAATT CTCAGAAGAC CTATGTTGTT CATTGCCATG ACTGCTCACG AAAATCATGC 
CCAAACCTAG AAAATTTTGT GGTGCTAGAA CAGTTCAAAA TGGAGGAGCT GATGCAGATC 
TATGACCTAT TTACATTAGC TCCTCCAATG CCTTCACCAT CATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811996.1
CDS	1089..5456
Translation	MKSCGVSLSAAAVVAAAAASLGDEKKKMAAGKASETEEDFPNLTAEEREALVGLDSRLYGFLRLHEDGARTKALLLKAIRCYEALILKSEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFHWAIRAFQEVLYVEPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDCNPCTLSKAEIQFHIAHLYETQKKYHSAKEAYEQLLQTENLPTQVKATVLQQLGWMHHTVDQLGDKATKASYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCNNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNSAQQACKPHHQNAESVLGLSQTPISQQSLPLHMIPSSQVDELSSPAKRKRTSSPAKNTSDTWSSGHLASHTPGQQQVHSWCLPPHKVQQLEQLRANRNNLNPAQKAALEQLEAQFVLMQQQQQMRQTGLSQVRSTGIANGPTADSTLPTNSLTGQQHQVALTRMPNAAQPGIRPACPAQPVANGPFPVGQVSCSTTGPVGSADTMLIGNNHITGNGSNGNVPYPQRNALALPHNRTNLTSSAEEPWKNQLTNSTQGLHKGQSSHLAGPNCERPPSSTGPSQHLQAAGTGIQNQVGHPTMPSNSVTQGAALNHLSSHTATSGGQQGIILTKESKPSGNGSVPETSRHSEGTPNSTAGVEGLPNHVHQVTADTVPSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNVHPAVHTKTENSVASSPSSAISTATPSPKSTEQTMTNSVVSLKSPLSGHDKVNGEQTEDSQSPTKKDHSLPSHKLSPQTIPSMSVSIYPSSVEVLKACRNLGRNGLSNSSILLDKCPPPRPPPPPYPPLPTDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHIVEVRTQLLQPADENWDPTGTKKIWRCESSRSHTTIAKYAQYQAASFQESLREENEKKSQHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDERKWKLQLHELTKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPETYWSVMHDFCEKNNINFLLGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTAFQYKLAVERYEWNKLQCVKSIVPMVHLSWNMAKNIKVSDPKLFEMIKYCLLRTLKHCQALREVLNASGIEIVWHGRTKDEPAHYCSNCEVEVFNLLFVTKESNSQKTYVVHCHDCSRKSCPNLENFVVLEQFKMEELMQIYDLFTLAPPMPSPSS

Target ORF information:

RefSeq Version	XM_012956542.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956542.2

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT GCTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACTCAGCAC AGCAGGCCTG TAAACCTCAT CATCAGAATG CTGAATCTGT ACTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCATTACACA TGATTCCTTC TAGCCAGGTA 
GATGAACTGT CAAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAGCAAA GAATACTTCT 
GATACCTGGA GCAGTGGCCA TTTGGCGTCA CATACTCCAG GACAGCAACA AGTACATTCG 
TGGTGTTTGC CGCCACACAA AGTACAGCAA TTGGAACAGC TACGAGCAAA CAGGAATAAT 
CTGAATCCAG CACAAAAAGC GGCGTTGGAG CAGTTGGAGG CTCAGTTTGT CTTAATGCAG 
CAGCAACAGC AGATGAGACA AACTGGACTT TCACAGGTAC GTTCTACTGG AATTGCTAAC 
GGGCCAACTG CTGACTCAAC ACTGCCTACA AACTCACTCA CTGGTCAGCA ACATCAGGTT 
GCATTAACCA GGATGCCTAA TGCCGCTCAG CCTGGAATCC GTCCGGCTTG CCCTGCGCAG 
CCTGTGGCTA ATGGACCATT TCCAGTAGGC CAAGTTTCCT GCAGTACCAC AGGGCCAGTG 
GGCAGTGCAG ACACTATGTT GATAGGCAAT AATCATATTA CGGGAAATGG AAGTAATGGA 
AATGTGCCTT ACCCGCAGCG AAACGCACTC GCACTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTAACTAACT CCACTCAGGG GCTTCATAAA 
GGTCAGAGTT CACATTTGGC AGGTCCTAAT TGTGAACGAC CTCCTTCTTC CACTGGGCCT 
TCCCAGCATC TCCAAGCAGC TGGCACTGGT ATACAAAATC AGGTTGGACA TCCTACCATG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TATTTTAACC AAAGAGAGCA AGCCTTCAGG AAATGGATCA 
GTGCCTGAAA CAAGCAGGCA TTCTGAGGGG ACACCTAACA GCACTGCCGG TGTAGAGGGA 
CTTCCTAATC ATGTCCATCA GGTGACGGCA GATACTGTTC CTAGCCCTAG CCATGGAGAT 
TCAAAGTCAC CAGGTTTACT TAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA 
AAAGCCAATA ACAATGTAGG TCCTGGAACC TGTGACAAGG TCAACAATGT TCATCCAGCT 
GTTCATACAA AAACTGAGAA TTCTGTTGCC TCCTCTCCAT CTTCAGCCAT TTCCACAGCA 
ACACCTTCTC CAAAATCTAC CGAACAGACT ATGACCAACA GTGTTGTCAG CCTTAAAAGC 
CCTCTCAGTG GACATGATAA AGTGAATGGA GAGCAAACGG AGGACTCTCA GAGCCCCACA 
AAAAAGGACC ACTCTTTACC TAGCCACAAA TTAAGTCCAC AGACCATACC ATCCATGTCT 
GTATCCATTT ACCCTAGCTC AGTAGAGGTT TTAAAGGCTT GTCGAAATCT GGGTAGAAAT 
GGTTTATCGA ACAGTAGTAT TTTACTGGAC AAGTGCCCAC CACCAAGGCC GCCGCCTCCA 
CCCTATCCAC CTTTACCAAC AGATAAATTA AATCCTCCAA CACCGAGCAT TTACTTAGAA 
AACAAGAGAG ATGCTTTCTT CCCACCACTC CATCAGTTCT GCACAAATCC AAACAACCCT 
GTTACTGTCA TACGAGGCCT TGCTGGAGCT CTTAAATTAG ATCTAGGACT ATTTTCTACA 
AAAACACTAG TAGAAGCGAA TAATGAACAT ATAGTTGAAG TGAGGACACA GTTATTACAG 
CCTGCAGACG AAAACTGGGA CCCCACTGGA ACAAAGAAAA TCTGGCGCTG TGAAAGCAGT 
CGTTCTCATA CCACCATTGC AAAATATGCA CAGTATCAGG CCGCTTCATT CCAAGAATCA 
TTAAGAGAGG AAAATGAGAA GAAAAGCCAA CACAAAGACC ATTCAGATAG TGAATCCACA 
TCTTCAGATA ACTCTGGTAG AAGACGTAAA GGCCCTTTTA AAACCATTAA ATTTGGGACC 
AATATTGATT TGTCTGATGA AAGAAAGTGG AAACTACAGC TTCATGAACT GACCAAACTT 
CCTGCGTTTG CACGTGTTGT GTCAGCTGGA AATCTTCTGA GTCATGTTGG CCATACTATA 
CTAGGAATGA ACACCGTTCA ACTCTACATG AAGGTTCCAG GCAGTAGGAC ACCAGGCCAT 
CAAGAGAACA ACAACTTCTG TTCGGTTAAT ATTAATATTG GTCCGGGAGA TTGTGAATGG 
TTTGTGGTAC CTGAGACGTA CTGGAGCGTA ATGCATGACT TCTGTGAAAA GAATAATATA 
AACTTCCTTT TGGGATCATG GTGGCCCAAT CTTGAAGACC TATATGAAGC AAATGTGCCA 
GTCTACAGGT TTATTCAAAG GCCTGGCGAC TTAGTTTGGA TTAATGCTGG CACAGTGCAT 
TGGGTTCAAG CAATTGGTTG GTGTAACAAC ATTGCATGGA ATGTTGGCCC TCTCACAGCC 
TTCCAGTATA AACTGGCAGT GGAACGGTAC GAATGGAATA AATTGCAGTG CGTCAAGTCT 
ATTGTGCCAA TGGTTCATCT CTCTTGGAAT ATGGCAAAGA ATATCAAAGT TTCAGATCCA 
AAGCTTTTTG AAATGATTAA ATATTGCTTA TTGAGGACCT TGAAGCACTG TCAAGCACTG 
AGAGAAGTTC TTAATGCTTC TGGAATTGAA ATTGTGTGGC ATGGCAGAAC GAAAGATGAG 
CCTGCTCATT ACTGTAGCAA CTGTGAGGTG GAAGTCTTCA ATTTACTTTT TGTGACAAAA 
GAAAGCAATT CTCAGAAGAC CTATGTTGTT CATTGCCATG ACTGCTCACG AAAATCATGC 
CCAAACCTAG AAAATTTTGT GGTGCTAGAA CAGTTCAAAA TGGAGGAGCT GATGCAGATC 
TATGACCTAT TTACATTAGC TCCTCCAATG CCTTCACCAT CATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_012956542.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811996.1 Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X1, mRNA.

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CloneID	OXa32428
Clone ID Related Accession (Same CDS sequence)	XM_012956542.2 , XM_012956542.3
Accession Version	XM_012956542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956542.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT GCTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACTCAGCAC AGCAGGCCTG TAAACCTCAT CATCAGAATG CTGAATCTGT ACTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCATTACACA TGATTCCTTC TAGCCAGGTA 
GATGAACTGT CAAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAGCAAA GAATACTTCT 
GATACCTGGA GCAGTGGCCA TTTGGCGTCA CATACTCCAG GACAGCAACA AGTACATTCG 
TGGTGTTTGC CGCCACACAA AGTACAGCAA TTGGAACAGC TACGAGCAAA CAGGAATAAT 
CTGAATCCAG CACAAAAAGC GGCGTTGGAG CAGTTGGAGG CTCAGTTTGT CTTAATGCAG 
CAGCAACAGC AGATGAGACA AACTGGACTT TCACAGGTAC GTTCTACTGG AATTGCTAAC 
GGGCCAACTG CTGACTCAAC ACTGCCTACA AACTCACTCA CTGGTCAGCA ACATCAGGTT 
GCATTAACCA GGATGCCTAA TGCCGCTCAG CCTGGAATCC GTCCGGCTTG CCCTGCGCAG 
CCTGTGGCTA ATGGACCATT TCCAGTAGGC CAAGTTTCCT GCAGTACCAC AGGGCCAGTG 
GGCAGTGCAG ACACTATGTT GATAGGCAAT AATCATATTA CGGGAAATGG AAGTAATGGA 
AATGTGCCTT ACCCGCAGCG AAACGCACTC GCACTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTAACTAACT CCACTCAGGG GCTTCATAAA 
GGTCAGAGTT CACATTTGGC AGGTCCTAAT TGTGAACGAC CTCCTTCTTC CACTGGGCCT 
TCCCAGCATC TCCAAGCAGC TGGCACTGGT ATACAAAATC AGGTTGGACA TCCTACCATG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TATTTTAACC AAAGAGAGCA AGCCTTCAGG AAATGGATCA 
GTGCCTGAAA CAAGCAGGCA TTCTGAGGGG ACACCTAACA GCACTGCCGG TGTAGAGGGA 
CTTCCTAATC ATGTCCATCA GGTGACGGCA GATACTGTTC CTAGCCCTAG CCATGGAGAT 
TCAAAGTCAC CAGGTTTACT TAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA 
AAAGCCAATA ACAATGTAGG TCCTGGAACC TGTGACAAGG TCAACAATGT TCATCCAGCT 
GTTCATACAA AAACTGAGAA TTCTGTTGCC TCCTCTCCAT CTTCAGCCAT TTCCACAGCA 
ACACCTTCTC CAAAATCTAC CGAACAGACT ATGACCAACA GTGTTGTCAG CCTTAAAAGC 
CCTCTCAGTG GACATGATAA AGTGAATGGA GAGCAAACGG AGGACTCTCA GAGCCCCACA 
AAAAAGGACC ACTCTTTACC TAGCCACAAA TTAAGTCCAC AGACCATACC ATCCATGTCT 
GTATCCATTT ACCCTAGCTC AGTAGAGGTT TTAAAGGCTT GTCGAAATCT GGGTAGAAAT 
GGTTTATCGA ACAGTAGTAT TTTACTGGAC AAGTGCCCAC CACCAAGGCC GCCGCCTCCA 
CCCTATCCAC CTTTACCAAC AGATAAATTA AATCCTCCAA CACCGAGCAT TTACTTAGAA 
AACAAGAGAG ATGCTTTCTT CCCACCACTC CATCAGTTCT GCACAAATCC AAACAACCCT 
GTTACTGTCA TACGAGGCCT TGCTGGAGCT CTTAAATTAG ATCTAGGACT ATTTTCTACA 
AAAACACTAG TAGAAGCGAA TAATGAACAT ATAGTTGAAG TGAGGACACA GTTATTACAG 
CCTGCAGACG AAAACTGGGA CCCCACTGGA ACAAAGAAAA TCTGGCGCTG TGAAAGCAGT 
CGTTCTCATA CCACCATTGC AAAATATGCA CAGTATCAGG CCGCTTCATT CCAAGAATCA 
TTAAGAGAGG AAAATGAGAA GAAAAGCCAA CACAAAGACC ATTCAGATAG TGAATCCACA 
TCTTCAGATA ACTCTGGTAG AAGACGTAAA GGCCCTTTTA AAACCATTAA ATTTGGGACC 
AATATTGATT TGTCTGATGA AAGAAAGTGG AAACTACAGC TTCATGAACT GACCAAACTT 
CCTGCGTTTG CACGTGTTGT GTCAGCTGGA AATCTTCTGA GTCATGTTGG CCATACTATA 
CTAGGAATGA ACACCGTTCA ACTCTACATG AAGGTTCCAG GCAGTAGGAC ACCAGGCCAT 
CAAGAGAACA ACAACTTCTG TTCGGTTAAT ATTAATATTG GTCCGGGAGA TTGTGAATGG 
TTTGTGGTAC CTGAGACGTA CTGGAGCGTA ATGCATGACT TCTGTGAAAA GAATAATATA 
AACTTCCTTT TGGGATCATG GTGGCCCAAT CTTGAAGACC TATATGAAGC AAATGTGCCA 
GTCTACAGGT TTATTCAAAG GCCTGGCGAC TTAGTTTGGA TTAATGCTGG CACAGTGCAT 
TGGGTTCAAG CAATTGGTTG GTGTAACAAC ATTGCATGGA ATGTTGGCCC TCTCACAGCC 
TTCCAGTATA AACTGGCAGT GGAACGGTAC GAATGGAATA AATTGCAGTG CGTCAAGTCT 
ATTGTGCCAA TGGTTCATCT CTCTTGGAAT ATGGCAAAGA ATATCAAAGT TTCAGATCCA 
AAGCTTTTTG AAATGATTAA ATATTGCTTA TTGAGGACCT TGAAGCACTG TCAAGCACTG 
AGAGAAGTTC TTAATGCTTC TGGAATTGAA ATTGTGTGGC ATGGCAGAAC GAAAGATGAG 
CCTGCTCATT ACTGTAGCAA CTGTGAGGTG GAAGTCTTCA ATTTACTTTT TGTGACAAAA 
GAAAGCAATT CTCAGAAGAC CTATGTTGTT CATTGCCATG ACTGCTCACG AAAATCATGC 
CCAAACCTAG AAAATTTTGT GGTGCTAGAA CAGTTCAAAA TGGAGGAGCT GATGCAGATC 
TATGACCTAT TTACATTAGC TCCTCCAATG CCTTCACCAT CATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811996.1
CDS	675..5042
Translation	MKSCGVSLSAAAVVAAAAASLGDEKKKMAAGKASETEEDFPNLTAEEREALVGLDSRLYGFLRLHEDGARTKALLLKAIRCYEALILKSEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFHWAIRAFQEVLYVEPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDCNPCTLSKAEIQFHIAHLYETQKKYHSAKEAYEQLLQTENLPTQVKATVLQQLGWMHHTVDQLGDKATKASYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCNNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNSAQQACKPHHQNAESVLGLSQTPISQQSLPLHMIPSSQVDELSSPAKRKRTSSPAKNTSDTWSSGHLASHTPGQQQVHSWCLPPHKVQQLEQLRANRNNLNPAQKAALEQLEAQFVLMQQQQQMRQTGLSQVRSTGIANGPTADSTLPTNSLTGQQHQVALTRMPNAAQPGIRPACPAQPVANGPFPVGQVSCSTTGPVGSADTMLIGNNHITGNGSNGNVPYPQRNALALPHNRTNLTSSAEEPWKNQLTNSTQGLHKGQSSHLAGPNCERPPSSTGPSQHLQAAGTGIQNQVGHPTMPSNSVTQGAALNHLSSHTATSGGQQGIILTKESKPSGNGSVPETSRHSEGTPNSTAGVEGLPNHVHQVTADTVPSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNVHPAVHTKTENSVASSPSSAISTATPSPKSTEQTMTNSVVSLKSPLSGHDKVNGEQTEDSQSPTKKDHSLPSHKLSPQTIPSMSVSIYPSSVEVLKACRNLGRNGLSNSSILLDKCPPPRPPPPPYPPLPTDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHIVEVRTQLLQPADENWDPTGTKKIWRCESSRSHTTIAKYAQYQAASFQESLREENEKKSQHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDERKWKLQLHELTKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPETYWSVMHDFCEKNNINFLLGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTAFQYKLAVERYEWNKLQCVKSIVPMVHLSWNMAKNIKVSDPKLFEMIKYCLLRTLKHCQALREVLNASGIEIVWHGRTKDEPAHYCSNCEVEVFNLLFVTKESNSQKTYVVHCHDCSRKSCPNLENFVVLEQFKMEELMQIYDLFTLAPPMPSPSS

Target ORF information:

RefSeq Version	XM_012956542.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956542.3

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT GCTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACTCAGCAC AGCAGGCCTG TAAACCTCAT CATCAGAATG CTGAATCTGT ACTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCATTACACA TGATTCCTTC TAGCCAGGTA 
GATGAACTGT CAAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAGCAAA GAATACTTCT 
GATACCTGGA GCAGTGGCCA TTTGGCGTCA CATACTCCAG GACAGCAACA AGTACATTCG 
TGGTGTTTGC CGCCACACAA AGTACAGCAA TTGGAACAGC TACGAGCAAA CAGGAATAAT 
CTGAATCCAG CACAAAAAGC GGCGTTGGAG CAGTTGGAGG CTCAGTTTGT CTTAATGCAG 
CAGCAACAGC AGATGAGACA AACTGGACTT TCACAGGTAC GTTCTACTGG AATTGCTAAC 
GGGCCAACTG CTGACTCAAC ACTGCCTACA AACTCACTCA CTGGTCAGCA ACATCAGGTT 
GCATTAACCA GGATGCCTAA TGCCGCTCAG CCTGGAATCC GTCCGGCTTG CCCTGCGCAG 
CCTGTGGCTA ATGGACCATT TCCAGTAGGC CAAGTTTCCT GCAGTACCAC AGGGCCAGTG 
GGCAGTGCAG ACACTATGTT GATAGGCAAT AATCATATTA CGGGAAATGG AAGTAATGGA 
AATGTGCCTT ACCCGCAGCG AAACGCACTC GCACTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTAACTAACT CCACTCAGGG GCTTCATAAA 
GGTCAGAGTT CACATTTGGC AGGTCCTAAT TGTGAACGAC CTCCTTCTTC CACTGGGCCT 
TCCCAGCATC TCCAAGCAGC TGGCACTGGT ATACAAAATC AGGTTGGACA TCCTACCATG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TATTTTAACC AAAGAGAGCA AGCCTTCAGG AAATGGATCA 
GTGCCTGAAA CAAGCAGGCA TTCTGAGGGG ACACCTAACA GCACTGCCGG TGTAGAGGGA 
CTTCCTAATC ATGTCCATCA GGTGACGGCA GATACTGTTC CTAGCCCTAG CCATGGAGAT 
TCAAAGTCAC CAGGTTTACT TAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA 
AAAGCCAATA ACAATGTAGG TCCTGGAACC TGTGACAAGG TCAACAATGT TCATCCAGCT 
GTTCATACAA AAACTGAGAA TTCTGTTGCC TCCTCTCCAT CTTCAGCCAT TTCCACAGCA 
ACACCTTCTC CAAAATCTAC CGAACAGACT ATGACCAACA GTGTTGTCAG CCTTAAAAGC 
CCTCTCAGTG GACATGATAA AGTGAATGGA GAGCAAACGG AGGACTCTCA GAGCCCCACA 
AAAAAGGACC ACTCTTTACC TAGCCACAAA TTAAGTCCAC AGACCATACC ATCCATGTCT 
GTATCCATTT ACCCTAGCTC AGTAGAGGTT TTAAAGGCTT GTCGAAATCT GGGTAGAAAT 
GGTTTATCGA ACAGTAGTAT TTTACTGGAC AAGTGCCCAC CACCAAGGCC GCCGCCTCCA 
CCCTATCCAC CTTTACCAAC AGATAAATTA AATCCTCCAA CACCGAGCAT TTACTTAGAA 
AACAAGAGAG ATGCTTTCTT CCCACCACTC CATCAGTTCT GCACAAATCC AAACAACCCT 
GTTACTGTCA TACGAGGCCT TGCTGGAGCT CTTAAATTAG ATCTAGGACT ATTTTCTACA 
AAAACACTAG TAGAAGCGAA TAATGAACAT ATAGTTGAAG TGAGGACACA GTTATTACAG 
CCTGCAGACG AAAACTGGGA CCCCACTGGA ACAAAGAAAA TCTGGCGCTG TGAAAGCAGT 
CGTTCTCATA CCACCATTGC AAAATATGCA CAGTATCAGG CCGCTTCATT CCAAGAATCA 
TTAAGAGAGG AAAATGAGAA GAAAAGCCAA CACAAAGACC ATTCAGATAG TGAATCCACA 
TCTTCAGATA ACTCTGGTAG AAGACGTAAA GGCCCTTTTA AAACCATTAA ATTTGGGACC 
AATATTGATT TGTCTGATGA AAGAAAGTGG AAACTACAGC TTCATGAACT GACCAAACTT 
CCTGCGTTTG CACGTGTTGT GTCAGCTGGA AATCTTCTGA GTCATGTTGG CCATACTATA 
CTAGGAATGA ACACCGTTCA ACTCTACATG AAGGTTCCAG GCAGTAGGAC ACCAGGCCAT 
CAAGAGAACA ACAACTTCTG TTCGGTTAAT ATTAATATTG GTCCGGGAGA TTGTGAATGG 
TTTGTGGTAC CTGAGACGTA CTGGAGCGTA ATGCATGACT TCTGTGAAAA GAATAATATA 
AACTTCCTTT TGGGATCATG GTGGCCCAAT CTTGAAGACC TATATGAAGC AAATGTGCCA 
GTCTACAGGT TTATTCAAAG GCCTGGCGAC TTAGTTTGGA TTAATGCTGG CACAGTGCAT 
TGGGTTCAAG CAATTGGTTG GTGTAACAAC ATTGCATGGA ATGTTGGCCC TCTCACAGCC 
TTCCAGTATA AACTGGCAGT GGAACGGTAC GAATGGAATA AATTGCAGTG CGTCAAGTCT 
ATTGTGCCAA TGGTTCATCT CTCTTGGAAT ATGGCAAAGA ATATCAAAGT TTCAGATCCA 
AAGCTTTTTG AAATGATTAA ATATTGCTTA TTGAGGACCT TGAAGCACTG TCAAGCACTG 
AGAGAAGTTC TTAATGCTTC TGGAATTGAA ATTGTGTGGC ATGGCAGAAC GAAAGATGAG 
CCTGCTCATT ACTGTAGCAA CTGTGAGGTG GAAGTCTTCA ATTTACTTTT TGTGACAAAA 
GAAAGCAATT CTCAGAAGAC CTATGTTGTT CATTGCCATG ACTGCTCACG AAAATCATGC 
CCAAACCTAG AAAATTTTGT GGTGCTAGAA CAGTTCAAAA TGGAGGAGCT GATGCAGATC 
TATGACCTAT TTACATTAGC TCCTCCAATG CCTTCACCAT CATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_012956544.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811998.1 Xenopus tropicalis lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.

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CloneID	OXa32429
Clone ID Related Accession (Same CDS sequence)	XM_012956544.2 , XM_012956544.3
Accession Version	XM_012956544.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCCTGTAAAC CTCATCATCA GAATGCTGAA TCTGTACTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCATT ACACATGATT CCTTCTAGCC AGGTAGATGA ACTGTCAAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA GCAAAGAATA CTTCTGATAC CTGGAGCAGT 
GGCCATTTGG CGTCACATAC TCCAGGACAG CAACAAGTAC ATTCGTGGTG TTTGCCGCCA 
CACAAAGTAC AGCAATTGGA ACAGCTACGA GCAAACAGGA ATAATCTGAA TCCAGCACAA 
AAAGCGGCGT TGGAGCAGTT GGAGGCTCAG TTTGTCTTAA TGCAGCAGCA ACAGCAGATG 
AGACAAACTG GACTTTCACA GGTACGTTCT ACTGGAATTG CTAACGGGCC AACTGCTGAC 
TCAACACTGC CTACAAACTC ACTCACTGGT CAGCAACATC AGGTTGCATT AACCAGGATG 
CCTAATGCCG CTCAGCCTGG AATCCGTCCG GCTTGCCCTG CGCAGCCTGT GGCTAATGGA 
CCATTTCCAG TAGGCCAAGT TTCCTGCAGT ACCACAGGGC CAGTGGGCAG TGCAGACACT 
ATGTTGATAG GCAATAATCA TATTACGGGA AATGGAAGTA ATGGAAATGT GCCTTACCCG 
CAGCGAAACG CACTCGCACT ACCTCATAAC CGCACAAACC TGACCAGCAG CGCAGAGGAG 
CCGTGGAAAA ACCAACTAAC TAACTCCACT CAGGGGCTTC ATAAAGGTCA GAGTTCACAT 
TTGGCAGGTC CTAATTGTGA ACGACCTCCT TCTTCCACTG GGCCTTCCCA GCATCTCCAA 
GCAGCTGGCA CTGGTATACA AAATCAGGTT GGACATCCTA CCATGCCTAG CAATTCAGTA 
ACACAGGGGG CTGCTCTCAA TCACCTCTCC TCTCACACTG CTACCTCAGG TGGACAACAA 
GGCATTATTT TAACCAAAGA GAGCAAGCCT TCAGGAAATG GATCAGTGCC TGAAACAAGC 
AGGCATTCTG AGGGGACACC TAACAGCACT GCCGGTGTAG AGGGACTTCC TAATCATGTC 
CATCAGGTGA CGGCAGATAC TGTTCCTAGC CCTAGCCATG GAGATTCAAA GTCACCAGGT 
TTACTTAGTT CAGACAATCC TCAGCTCTCT GCCTTGTTGA TGGGAAAAGC CAATAACAAT 
GTAGGTCCTG GAACCTGTGA CAAGGTCAAC AATGTTCATC CAGCTGTTCA TACAAAAACT 
GAGAATTCTG TTGCCTCCTC TCCATCTTCA GCCATTTCCA CAGCAACACC TTCTCCAAAA 
TCTACCGAAC AGACTATGAC CAACAGTGTT GTCAGCCTTA AAAGCCCTCT CAGTGGACAT 
GATAAAGTGA ATGGAGAGCA AACGGAGGAC TCTCAGAGCC CCACAAAAAA GGACCACTCT 
TTACCTAGCC ACAAATTAAG TCCACAGACC ATACCATCCA TGTCTGTATC CATTTACCCT 
AGCTCAGTAG AGGTTTTAAA GGCTTGTCGA AATCTGGGTA GAAATGGTTT ATCGAACAGT 
AGTATTTTAC TGGACAAGTG CCCACCACCA AGGCCGCCGC CTCCACCCTA TCCACCTTTA 
CCAACAGATA AATTAAATCC TCCAACACCG AGCATTTACT TAGAAAACAA GAGAGATGCT 
TTCTTCCCAC CACTCCATCA GTTCTGCACA AATCCAAACA ACCCTGTTAC TGTCATACGA 
GGCCTTGCTG GAGCTCTTAA ATTAGATCTA GGACTATTTT CTACAAAAAC ACTAGTAGAA 
GCGAATAATG AACATATAGT TGAAGTGAGG ACACAGTTAT TACAGCCTGC AGACGAAAAC 
TGGGACCCCA CTGGAACAAA GAAAATCTGG CGCTGTGAAA GCAGTCGTTC TCATACCACC 
ATTGCAAAAT ATGCACAGTA TCAGGCCGCT TCATTCCAAG AATCATTAAG AGAGGAAAAT 
GAGAAGAAAA GCCAACACAA AGACCATTCA GATAGTGAAT CCACATCTTC AGATAACTCT 
GGTAGAAGAC GTAAAGGCCC TTTTAAAACC ATTAAATTTG GGACCAATAT TGATTTGTCT 
GATGAAAGAA AGTGGAAACT ACAGCTTCAT GAACTGACCA AACTTCCTGC GTTTGCACGT 
GTTGTGTCAG CTGGAAATCT TCTGAGTCAT GTTGGCCATA CTATACTAGG AATGAACACC 
GTTCAACTCT ACATGAAGGT TCCAGGCAGT AGGACACCAG GCCATCAAGA GAACAACAAC 
TTCTGTTCGG TTAATATTAA TATTGGTCCG GGAGATTGTG AATGGTTTGT GGTACCTGAG 
ACGTACTGGA GCGTAATGCA TGACTTCTGT GAAAAGAATA ATATAAACTT CCTTTTGGGA 
TCATGGTGGC CCAATCTTGA AGACCTATAT GAAGCAAATG TGCCAGTCTA CAGGTTTATT 
CAAAGGCCTG GCGACTTAGT TTGGATTAAT GCTGGCACAG TGCATTGGGT TCAAGCAATT 
GGTTGGTGTA ACAACATTGC ATGGAATGTT GGCCCTCTCA CAGCCTTCCA GTATAAACTG 
GCAGTGGAAC GGTACGAATG GAATAAATTG CAGTGCGTCA AGTCTATTGT GCCAATGGTT 
CATCTCTCTT GGAATATGGC AAAGAATATC AAAGTTTCAG ATCCAAAGCT TTTTGAAATG 
ATTAAATATT GCTTATTGAG GACCTTGAAG CACTGTCAAG CACTGAGAGA AGTTCTTAAT 
GCTTCTGGAA TTGAAATTGT GTGGCATGGC AGAACGAAAG ATGAGCCTGC TCATTACTGT 
AGCAACTGTG AGGTGGAAGT CTTCAATTTA CTTTTTGTGA CAAAAGAAAG CAATTCTCAG 
AAGACCTATG TTGTTCATTG CCATGACTGC TCACGAAAAT CATGCCCAAA CCTAGAAAAT 
TTTGTGGTGC TAGAACAGTT CAAAATGGAG GAGCTGATGC AGATCTATGA CCTATTTACA 
TTAGCTCCTC CAATGCCTTC ACCATCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811998.1
CDS	1088..5320
Translation	MKSCGVSLSAAAVVAAAAASLGDEKKKMAAGKASETEEDFPNLTAEEREALVGLDSRLYGFLRLHEDGARTKALLLKAIRCYEALILKSEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFHWAIRAFQEVLYVEPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDCNPCTLSKAEIQFHIAHLYETQKKYHSAKEAYEQLLQTENLPTQVKATVLQQLGWMHHTVDQLGDKATKASYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCNNTSALAARIKYLQACKPHHQNAESVLGLSQTPISQQSLPLHMIPSSQVDELSSPAKRKRTSSPAKNTSDTWSSGHLASHTPGQQQVHSWCLPPHKVQQLEQLRANRNNLNPAQKAALEQLEAQFVLMQQQQQMRQTGLSQVRSTGIANGPTADSTLPTNSLTGQQHQVALTRMPNAAQPGIRPACPAQPVANGPFPVGQVSCSTTGPVGSADTMLIGNNHITGNGSNGNVPYPQRNALALPHNRTNLTSSAEEPWKNQLTNSTQGLHKGQSSHLAGPNCERPPSSTGPSQHLQAAGTGIQNQVGHPTMPSNSVTQGAALNHLSSHTATSGGQQGIILTKESKPSGNGSVPETSRHSEGTPNSTAGVEGLPNHVHQVTADTVPSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNVHPAVHTKTENSVASSPSSAISTATPSPKSTEQTMTNSVVSLKSPLSGHDKVNGEQTEDSQSPTKKDHSLPSHKLSPQTIPSMSVSIYPSSVEVLKACRNLGRNGLSNSSILLDKCPPPRPPPPPYPPLPTDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHIVEVRTQLLQPADENWDPTGTKKIWRCESSRSHTTIAKYAQYQAASFQESLREENEKKSQHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDERKWKLQLHELTKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPETYWSVMHDFCEKNNINFLLGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTAFQYKLAVERYEWNKLQCVKSIVPMVHLSWNMAKNIKVSDPKLFEMIKYCLLRTLKHCQALREVLNASGIEIVWHGRTKDEPAHYCSNCEVEVFNLLFVTKESNSQKTYVVHCHDCSRKSCPNLENFVVLEQFKMEELMQIYDLFTLAPPMPSPSS

Target ORF information:

RefSeq Version	XM_012956544.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956544.2

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCCTGTAAAC CTCATCATCA GAATGCTGAA TCTGTACTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCATT ACACATGATT CCTTCTAGCC AGGTAGATGA ACTGTCAAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA GCAAAGAATA CTTCTGATAC CTGGAGCAGT 
GGCCATTTGG CGTCACATAC TCCAGGACAG CAACAAGTAC ATTCGTGGTG TTTGCCGCCA 
CACAAAGTAC AGCAATTGGA ACAGCTACGA GCAAACAGGA ATAATCTGAA TCCAGCACAA 
AAAGCGGCGT TGGAGCAGTT GGAGGCTCAG TTTGTCTTAA TGCAGCAGCA ACAGCAGATG 
AGACAAACTG GACTTTCACA GGTACGTTCT ACTGGAATTG CTAACGGGCC AACTGCTGAC 
TCAACACTGC CTACAAACTC ACTCACTGGT CAGCAACATC AGGTTGCATT AACCAGGATG 
CCTAATGCCG CTCAGCCTGG AATCCGTCCG GCTTGCCCTG CGCAGCCTGT GGCTAATGGA 
CCATTTCCAG TAGGCCAAGT TTCCTGCAGT ACCACAGGGC CAGTGGGCAG TGCAGACACT 
ATGTTGATAG GCAATAATCA TATTACGGGA AATGGAAGTA ATGGAAATGT GCCTTACCCG 
CAGCGAAACG CACTCGCACT ACCTCATAAC CGCACAAACC TGACCAGCAG CGCAGAGGAG 
CCGTGGAAAA ACCAACTAAC TAACTCCACT CAGGGGCTTC ATAAAGGTCA GAGTTCACAT 
TTGGCAGGTC CTAATTGTGA ACGACCTCCT TCTTCCACTG GGCCTTCCCA GCATCTCCAA 
GCAGCTGGCA CTGGTATACA AAATCAGGTT GGACATCCTA CCATGCCTAG CAATTCAGTA 
ACACAGGGGG CTGCTCTCAA TCACCTCTCC TCTCACACTG CTACCTCAGG TGGACAACAA 
GGCATTATTT TAACCAAAGA GAGCAAGCCT TCAGGAAATG GATCAGTGCC TGAAACAAGC 
AGGCATTCTG AGGGGACACC TAACAGCACT GCCGGTGTAG AGGGACTTCC TAATCATGTC 
CATCAGGTGA CGGCAGATAC TGTTCCTAGC CCTAGCCATG GAGATTCAAA GTCACCAGGT 
TTACTTAGTT CAGACAATCC TCAGCTCTCT GCCTTGTTGA TGGGAAAAGC CAATAACAAT 
GTAGGTCCTG GAACCTGTGA CAAGGTCAAC AATGTTCATC CAGCTGTTCA TACAAAAACT 
GAGAATTCTG TTGCCTCCTC TCCATCTTCA GCCATTTCCA CAGCAACACC TTCTCCAAAA 
TCTACCGAAC AGACTATGAC CAACAGTGTT GTCAGCCTTA AAAGCCCTCT CAGTGGACAT 
GATAAAGTGA ATGGAGAGCA AACGGAGGAC TCTCAGAGCC CCACAAAAAA GGACCACTCT 
TTACCTAGCC ACAAATTAAG TCCACAGACC ATACCATCCA TGTCTGTATC CATTTACCCT 
AGCTCAGTAG AGGTTTTAAA GGCTTGTCGA AATCTGGGTA GAAATGGTTT ATCGAACAGT 
AGTATTTTAC TGGACAAGTG CCCACCACCA AGGCCGCCGC CTCCACCCTA TCCACCTTTA 
CCAACAGATA AATTAAATCC TCCAACACCG AGCATTTACT TAGAAAACAA GAGAGATGCT 
TTCTTCCCAC CACTCCATCA GTTCTGCACA AATCCAAACA ACCCTGTTAC TGTCATACGA 
GGCCTTGCTG GAGCTCTTAA ATTAGATCTA GGACTATTTT CTACAAAAAC ACTAGTAGAA 
GCGAATAATG AACATATAGT TGAAGTGAGG ACACAGTTAT TACAGCCTGC AGACGAAAAC 
TGGGACCCCA CTGGAACAAA GAAAATCTGG CGCTGTGAAA GCAGTCGTTC TCATACCACC 
ATTGCAAAAT ATGCACAGTA TCAGGCCGCT TCATTCCAAG AATCATTAAG AGAGGAAAAT 
GAGAAGAAAA GCCAACACAA AGACCATTCA GATAGTGAAT CCACATCTTC AGATAACTCT 
GGTAGAAGAC GTAAAGGCCC TTTTAAAACC ATTAAATTTG GGACCAATAT TGATTTGTCT 
GATGAAAGAA AGTGGAAACT ACAGCTTCAT GAACTGACCA AACTTCCTGC GTTTGCACGT 
GTTGTGTCAG CTGGAAATCT TCTGAGTCAT GTTGGCCATA CTATACTAGG AATGAACACC 
GTTCAACTCT ACATGAAGGT TCCAGGCAGT AGGACACCAG GCCATCAAGA GAACAACAAC 
TTCTGTTCGG TTAATATTAA TATTGGTCCG GGAGATTGTG AATGGTTTGT GGTACCTGAG 
ACGTACTGGA GCGTAATGCA TGACTTCTGT GAAAAGAATA ATATAAACTT CCTTTTGGGA 
TCATGGTGGC CCAATCTTGA AGACCTATAT GAAGCAAATG TGCCAGTCTA CAGGTTTATT 
CAAAGGCCTG GCGACTTAGT TTGGATTAAT GCTGGCACAG TGCATTGGGT TCAAGCAATT 
GGTTGGTGTA ACAACATTGC ATGGAATGTT GGCCCTCTCA CAGCCTTCCA GTATAAACTG 
GCAGTGGAAC GGTACGAATG GAATAAATTG CAGTGCGTCA AGTCTATTGT GCCAATGGTT 
CATCTCTCTT GGAATATGGC AAAGAATATC AAAGTTTCAG ATCCAAAGCT TTTTGAAATG 
ATTAAATATT GCTTATTGAG GACCTTGAAG CACTGTCAAG CACTGAGAGA AGTTCTTAAT 
GCTTCTGGAA TTGAAATTGT GTGGCATGGC AGAACGAAAG ATGAGCCTGC TCATTACTGT 
AGCAACTGTG AGGTGGAAGT CTTCAATTTA CTTTTTGTGA CAAAAGAAAG CAATTCTCAG 
AAGACCTATG TTGTTCATTG CCATGACTGC TCACGAAAAT CATGCCCAAA CCTAGAAAAT 
TTTGTGGTGC TAGAACAGTT CAAAATGGAG GAGCTGATGC AGATCTATGA CCTATTTACA 
TTAGCTCCTC CAATGCCTTC ACCATCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_012956544.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811998.1 Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X2, mRNA.

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CloneID	OXa32429
Clone ID Related Accession (Same CDS sequence)	XM_012956544.2 , XM_012956544.3
Accession Version	XM_012956544.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956544.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCCTGTAAAC CTCATCATCA GAATGCTGAA TCTGTACTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCATT ACACATGATT CCTTCTAGCC AGGTAGATGA ACTGTCAAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA GCAAAGAATA CTTCTGATAC CTGGAGCAGT 
GGCCATTTGG CGTCACATAC TCCAGGACAG CAACAAGTAC ATTCGTGGTG TTTGCCGCCA 
CACAAAGTAC AGCAATTGGA ACAGCTACGA GCAAACAGGA ATAATCTGAA TCCAGCACAA 
AAAGCGGCGT TGGAGCAGTT GGAGGCTCAG TTTGTCTTAA TGCAGCAGCA ACAGCAGATG 
AGACAAACTG GACTTTCACA GGTACGTTCT ACTGGAATTG CTAACGGGCC AACTGCTGAC 
TCAACACTGC CTACAAACTC ACTCACTGGT CAGCAACATC AGGTTGCATT AACCAGGATG 
CCTAATGCCG CTCAGCCTGG AATCCGTCCG GCTTGCCCTG CGCAGCCTGT GGCTAATGGA 
CCATTTCCAG TAGGCCAAGT TTCCTGCAGT ACCACAGGGC CAGTGGGCAG TGCAGACACT 
ATGTTGATAG GCAATAATCA TATTACGGGA AATGGAAGTA ATGGAAATGT GCCTTACCCG 
CAGCGAAACG CACTCGCACT ACCTCATAAC CGCACAAACC TGACCAGCAG CGCAGAGGAG 
CCGTGGAAAA ACCAACTAAC TAACTCCACT CAGGGGCTTC ATAAAGGTCA GAGTTCACAT 
TTGGCAGGTC CTAATTGTGA ACGACCTCCT TCTTCCACTG GGCCTTCCCA GCATCTCCAA 
GCAGCTGGCA CTGGTATACA AAATCAGGTT GGACATCCTA CCATGCCTAG CAATTCAGTA 
ACACAGGGGG CTGCTCTCAA TCACCTCTCC TCTCACACTG CTACCTCAGG TGGACAACAA 
GGCATTATTT TAACCAAAGA GAGCAAGCCT TCAGGAAATG GATCAGTGCC TGAAACAAGC 
AGGCATTCTG AGGGGACACC TAACAGCACT GCCGGTGTAG AGGGACTTCC TAATCATGTC 
CATCAGGTGA CGGCAGATAC TGTTCCTAGC CCTAGCCATG GAGATTCAAA GTCACCAGGT 
TTACTTAGTT CAGACAATCC TCAGCTCTCT GCCTTGTTGA TGGGAAAAGC CAATAACAAT 
GTAGGTCCTG GAACCTGTGA CAAGGTCAAC AATGTTCATC CAGCTGTTCA TACAAAAACT 
GAGAATTCTG TTGCCTCCTC TCCATCTTCA GCCATTTCCA CAGCAACACC TTCTCCAAAA 
TCTACCGAAC AGACTATGAC CAACAGTGTT GTCAGCCTTA AAAGCCCTCT CAGTGGACAT 
GATAAAGTGA ATGGAGAGCA AACGGAGGAC TCTCAGAGCC CCACAAAAAA GGACCACTCT 
TTACCTAGCC ACAAATTAAG TCCACAGACC ATACCATCCA TGTCTGTATC CATTTACCCT 
AGCTCAGTAG AGGTTTTAAA GGCTTGTCGA AATCTGGGTA GAAATGGTTT ATCGAACAGT 
AGTATTTTAC TGGACAAGTG CCCACCACCA AGGCCGCCGC CTCCACCCTA TCCACCTTTA 
CCAACAGATA AATTAAATCC TCCAACACCG AGCATTTACT TAGAAAACAA GAGAGATGCT 
TTCTTCCCAC CACTCCATCA GTTCTGCACA AATCCAAACA ACCCTGTTAC TGTCATACGA 
GGCCTTGCTG GAGCTCTTAA ATTAGATCTA GGACTATTTT CTACAAAAAC ACTAGTAGAA 
GCGAATAATG AACATATAGT TGAAGTGAGG ACACAGTTAT TACAGCCTGC AGACGAAAAC 
TGGGACCCCA CTGGAACAAA GAAAATCTGG CGCTGTGAAA GCAGTCGTTC TCATACCACC 
ATTGCAAAAT ATGCACAGTA TCAGGCCGCT TCATTCCAAG AATCATTAAG AGAGGAAAAT 
GAGAAGAAAA GCCAACACAA AGACCATTCA GATAGTGAAT CCACATCTTC AGATAACTCT 
GGTAGAAGAC GTAAAGGCCC TTTTAAAACC ATTAAATTTG GGACCAATAT TGATTTGTCT 
GATGAAAGAA AGTGGAAACT ACAGCTTCAT GAACTGACCA AACTTCCTGC GTTTGCACGT 
GTTGTGTCAG CTGGAAATCT TCTGAGTCAT GTTGGCCATA CTATACTAGG AATGAACACC 
GTTCAACTCT ACATGAAGGT TCCAGGCAGT AGGACACCAG GCCATCAAGA GAACAACAAC 
TTCTGTTCGG TTAATATTAA TATTGGTCCG GGAGATTGTG AATGGTTTGT GGTACCTGAG 
ACGTACTGGA GCGTAATGCA TGACTTCTGT GAAAAGAATA ATATAAACTT CCTTTTGGGA 
TCATGGTGGC CCAATCTTGA AGACCTATAT GAAGCAAATG TGCCAGTCTA CAGGTTTATT 
CAAAGGCCTG GCGACTTAGT TTGGATTAAT GCTGGCACAG TGCATTGGGT TCAAGCAATT 
GGTTGGTGTA ACAACATTGC ATGGAATGTT GGCCCTCTCA CAGCCTTCCA GTATAAACTG 
GCAGTGGAAC GGTACGAATG GAATAAATTG CAGTGCGTCA AGTCTATTGT GCCAATGGTT 
CATCTCTCTT GGAATATGGC AAAGAATATC AAAGTTTCAG ATCCAAAGCT TTTTGAAATG 
ATTAAATATT GCTTATTGAG GACCTTGAAG CACTGTCAAG CACTGAGAGA AGTTCTTAAT 
GCTTCTGGAA TTGAAATTGT GTGGCATGGC AGAACGAAAG ATGAGCCTGC TCATTACTGT 
AGCAACTGTG AGGTGGAAGT CTTCAATTTA CTTTTTGTGA CAAAAGAAAG CAATTCTCAG 
AAGACCTATG TTGTTCATTG CCATGACTGC TCACGAAAAT CATGCCCAAA CCTAGAAAAT 
TTTGTGGTGC TAGAACAGTT CAAAATGGAG GAGCTGATGC AGATCTATGA CCTATTTACA 
TTAGCTCCTC CAATGCCTTC ACCATCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811998.1
CDS	675..4907
Translation	MKSCGVSLSAAAVVAAAAASLGDEKKKMAAGKASETEEDFPNLTAEEREALVGLDSRLYGFLRLHEDGARTKALLLKAIRCYEALILKSEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFHWAIRAFQEVLYVEPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDCNPCTLSKAEIQFHIAHLYETQKKYHSAKEAYEQLLQTENLPTQVKATVLQQLGWMHHTVDQLGDKATKASYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCNNTSALAARIKYLQACKPHHQNAESVLGLSQTPISQQSLPLHMIPSSQVDELSSPAKRKRTSSPAKNTSDTWSSGHLASHTPGQQQVHSWCLPPHKVQQLEQLRANRNNLNPAQKAALEQLEAQFVLMQQQQQMRQTGLSQVRSTGIANGPTADSTLPTNSLTGQQHQVALTRMPNAAQPGIRPACPAQPVANGPFPVGQVSCSTTGPVGSADTMLIGNNHITGNGSNGNVPYPQRNALALPHNRTNLTSSAEEPWKNQLTNSTQGLHKGQSSHLAGPNCERPPSSTGPSQHLQAAGTGIQNQVGHPTMPSNSVTQGAALNHLSSHTATSGGQQGIILTKESKPSGNGSVPETSRHSEGTPNSTAGVEGLPNHVHQVTADTVPSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNVHPAVHTKTENSVASSPSSAISTATPSPKSTEQTMTNSVVSLKSPLSGHDKVNGEQTEDSQSPTKKDHSLPSHKLSPQTIPSMSVSIYPSSVEVLKACRNLGRNGLSNSSILLDKCPPPRPPPPPYPPLPTDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHIVEVRTQLLQPADENWDPTGTKKIWRCESSRSHTTIAKYAQYQAASFQESLREENEKKSQHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDERKWKLQLHELTKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPETYWSVMHDFCEKNNINFLLGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTAFQYKLAVERYEWNKLQCVKSIVPMVHLSWNMAKNIKVSDPKLFEMIKYCLLRTLKHCQALREVLNASGIEIVWHGRTKDEPAHYCSNCEVEVFNLLFVTKESNSQKTYVVHCHDCSRKSCPNLENFVVLEQFKMEELMQIYDLFTLAPPMPSPSS

Target ORF information:

RefSeq Version	XM_012956544.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956544.3

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCCTGTAAAC CTCATCATCA GAATGCTGAA TCTGTACTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCATT ACACATGATT CCTTCTAGCC AGGTAGATGA ACTGTCAAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA GCAAAGAATA CTTCTGATAC CTGGAGCAGT 
GGCCATTTGG CGTCACATAC TCCAGGACAG CAACAAGTAC ATTCGTGGTG TTTGCCGCCA 
CACAAAGTAC AGCAATTGGA ACAGCTACGA GCAAACAGGA ATAATCTGAA TCCAGCACAA 
AAAGCGGCGT TGGAGCAGTT GGAGGCTCAG TTTGTCTTAA TGCAGCAGCA ACAGCAGATG 
AGACAAACTG GACTTTCACA GGTACGTTCT ACTGGAATTG CTAACGGGCC AACTGCTGAC 
TCAACACTGC CTACAAACTC ACTCACTGGT CAGCAACATC AGGTTGCATT AACCAGGATG 
CCTAATGCCG CTCAGCCTGG AATCCGTCCG GCTTGCCCTG CGCAGCCTGT GGCTAATGGA 
CCATTTCCAG TAGGCCAAGT TTCCTGCAGT ACCACAGGGC CAGTGGGCAG TGCAGACACT 
ATGTTGATAG GCAATAATCA TATTACGGGA AATGGAAGTA ATGGAAATGT GCCTTACCCG 
CAGCGAAACG CACTCGCACT ACCTCATAAC CGCACAAACC TGACCAGCAG CGCAGAGGAG 
CCGTGGAAAA ACCAACTAAC TAACTCCACT CAGGGGCTTC ATAAAGGTCA GAGTTCACAT 
TTGGCAGGTC CTAATTGTGA ACGACCTCCT TCTTCCACTG GGCCTTCCCA GCATCTCCAA 
GCAGCTGGCA CTGGTATACA AAATCAGGTT GGACATCCTA CCATGCCTAG CAATTCAGTA 
ACACAGGGGG CTGCTCTCAA TCACCTCTCC TCTCACACTG CTACCTCAGG TGGACAACAA 
GGCATTATTT TAACCAAAGA GAGCAAGCCT TCAGGAAATG GATCAGTGCC TGAAACAAGC 
AGGCATTCTG AGGGGACACC TAACAGCACT GCCGGTGTAG AGGGACTTCC TAATCATGTC 
CATCAGGTGA CGGCAGATAC TGTTCCTAGC CCTAGCCATG GAGATTCAAA GTCACCAGGT 
TTACTTAGTT CAGACAATCC TCAGCTCTCT GCCTTGTTGA TGGGAAAAGC CAATAACAAT 
GTAGGTCCTG GAACCTGTGA CAAGGTCAAC AATGTTCATC CAGCTGTTCA TACAAAAACT 
GAGAATTCTG TTGCCTCCTC TCCATCTTCA GCCATTTCCA CAGCAACACC TTCTCCAAAA 
TCTACCGAAC AGACTATGAC CAACAGTGTT GTCAGCCTTA AAAGCCCTCT CAGTGGACAT 
GATAAAGTGA ATGGAGAGCA AACGGAGGAC TCTCAGAGCC CCACAAAAAA GGACCACTCT 
TTACCTAGCC ACAAATTAAG TCCACAGACC ATACCATCCA TGTCTGTATC CATTTACCCT 
AGCTCAGTAG AGGTTTTAAA GGCTTGTCGA AATCTGGGTA GAAATGGTTT ATCGAACAGT 
AGTATTTTAC TGGACAAGTG CCCACCACCA AGGCCGCCGC CTCCACCCTA TCCACCTTTA 
CCAACAGATA AATTAAATCC TCCAACACCG AGCATTTACT TAGAAAACAA GAGAGATGCT 
TTCTTCCCAC CACTCCATCA GTTCTGCACA AATCCAAACA ACCCTGTTAC TGTCATACGA 
GGCCTTGCTG GAGCTCTTAA ATTAGATCTA GGACTATTTT CTACAAAAAC ACTAGTAGAA 
GCGAATAATG AACATATAGT TGAAGTGAGG ACACAGTTAT TACAGCCTGC AGACGAAAAC 
TGGGACCCCA CTGGAACAAA GAAAATCTGG CGCTGTGAAA GCAGTCGTTC TCATACCACC 
ATTGCAAAAT ATGCACAGTA TCAGGCCGCT TCATTCCAAG AATCATTAAG AGAGGAAAAT 
GAGAAGAAAA GCCAACACAA AGACCATTCA GATAGTGAAT CCACATCTTC AGATAACTCT 
GGTAGAAGAC GTAAAGGCCC TTTTAAAACC ATTAAATTTG GGACCAATAT TGATTTGTCT 
GATGAAAGAA AGTGGAAACT ACAGCTTCAT GAACTGACCA AACTTCCTGC GTTTGCACGT 
GTTGTGTCAG CTGGAAATCT TCTGAGTCAT GTTGGCCATA CTATACTAGG AATGAACACC 
GTTCAACTCT ACATGAAGGT TCCAGGCAGT AGGACACCAG GCCATCAAGA GAACAACAAC 
TTCTGTTCGG TTAATATTAA TATTGGTCCG GGAGATTGTG AATGGTTTGT GGTACCTGAG 
ACGTACTGGA GCGTAATGCA TGACTTCTGT GAAAAGAATA ATATAAACTT CCTTTTGGGA 
TCATGGTGGC CCAATCTTGA AGACCTATAT GAAGCAAATG TGCCAGTCTA CAGGTTTATT 
CAAAGGCCTG GCGACTTAGT TTGGATTAAT GCTGGCACAG TGCATTGGGT TCAAGCAATT 
GGTTGGTGTA ACAACATTGC ATGGAATGTT GGCCCTCTCA CAGCCTTCCA GTATAAACTG 
GCAGTGGAAC GGTACGAATG GAATAAATTG CAGTGCGTCA AGTCTATTGT GCCAATGGTT 
CATCTCTCTT GGAATATGGC AAAGAATATC AAAGTTTCAG ATCCAAAGCT TTTTGAAATG 
ATTAAATATT GCTTATTGAG GACCTTGAAG CACTGTCAAG CACTGAGAGA AGTTCTTAAT 
GCTTCTGGAA TTGAAATTGT GTGGCATGGC AGAACGAAAG ATGAGCCTGC TCATTACTGT 
AGCAACTGTG AGGTGGAAGT CTTCAATTTA CTTTTTGTGA CAAAAGAAAG CAATTCTCAG 
AAGACCTATG TTGTTCATTG CCATGACTGC TCACGAAAAT CATGCCCAAA CCTAGAAAAT 
TTTGTGGTGC TAGAACAGTT CAAAATGGAG GAGCTGATGC AGATCTATGA CCTATTTACA 
TTAGCTCCTC CAATGCCTTC ACCATCATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_031895734.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751594.1 Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa32430
Clone ID Related Accession (Same CDS sequence)	XM_031895734.1
Accession Version	XM_031895734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT GCTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACTCAGCAC AGCAGGCCTG TAAACCTCAT CATCAGAATG CTGAATCTGT ACTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCATTACACA TGATTCCTTC TAGCCAGGTA 
GATGAACTGT CAAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAGCAAA GAATACTTCT 
GATACCTGGA GCAGTGGCCA TTTGGCGTCA CATACTCCAG GACAGCAACA AGTACATTCG 
TGGTGTTTGC CGCCACACAA AGTACAGCAA TTGGAACAGC TACGAGCAAA CAGGAATAAT 
CTGAATCCAG CACAAAAAGC GGCGTTGGAG CAGTTGGAGG CTCAGTTTGT CTTAATGCAG 
CAGCAACAGC AGATGAGACA AACTGGACTT TCACAGCGAA ACGCACTCGC ACTACCTCAT 
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT AACTAACTCC 
ACTCAGGGGC TTCATAAAGG TCAGAGTTCA CATTTGGCAG GTCCTAATTG TGAACGACCT 
CCTTCTTCCA CTGGGCCTTC CCAGCATCTC CAAGCAGCTG GCACTGGTAT ACAAAATCAG 
GTTGGACATC CTACCATGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC 
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA TTTTAACCAA AGAGAGCAAG 
CCTTCAGGAA ATGGATCAGT GCCTGAAACA AGCAGGCATT CTGAGGGGAC ACCTAACAGC 
ACTGCCGGTG TAGAGGGACT TCCTAATCAT GTCCATCAGG TGACGGCAGA TACTGTTCCT 
AGCCCTAGCC ATGGAGATTC AAAGTCACCA GGTTTACTTA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTAGGTC CTGGAACCTG TGACAAGGTC 
AACAATGTTC ATCCAGCTGT TCATACAAAA ACTGAGAATT CTGTTGCCTC CTCTCCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCA AAATCTACCG AACAGACTAT GACCAACAGT 
GTTGTCAGCC TTAAAAGCCC TCTCAGTGGA CATGATAAAG TGAATGGAGA GCAAACGGAG 
GACTCTCAGA GCCCCACAAA AAAGGACCAC TCTTTACCTA GCCACAAATT AAGTCCACAG 
ACCATACCAT CCATGTCTGT ATCCATTTAC CCTAGCTCAG TAGAGGTTTT AAAGGCTTGT 
CGAAATCTGG GTAGAAATGG TTTATCGAAC AGTAGTATTT TACTGGACAA GTGCCCACCA 
CCAAGGCCGC CGCCTCCACC CTATCCACCT TTACCAACAG ATAAATTAAA TCCTCCAACA 
CCGAGCATTT ACTTAGAAAA CAAGAGAGAT GCTTTCTTCC CACCACTCCA TCAGTTCTGC 
ACAAATCCAA ACAACCCTGT TACTGTCATA CGAGGCCTTG CTGGAGCTCT TAAATTAGAT 
CTAGGACTAT TTTCTACAAA AACACTAGTA GAAGCGAATA ATGAACATAT AGTTGAAGTG 
AGGACACAGT TATTACAGCC TGCAGACGAA AACTGGGACC CCACTGGAAC AAAGAAAATC 
TGGCGCTGTG AAAGCAGTCG TTCTCATACC ACCATTGCAA AATATGCACA GTATCAGGCC 
GCTTCATTCC AAGAATCATT AAGAGAGGAA AATGAGAAGA AAAGCCAACA CAAAGACCAT 
TCAGATAGTG AATCCACATC TTCAGATAAC TCTGGTAGAA GACGTAAAGG CCCTTTTAAA 
ACCATTAAAT TTGGGACCAA TATTGATTTG TCTGATGAAA GAAAGTGGAA ACTACAGCTT 
CATGAACTGA CCAAACTTCC TGCGTTTGCA CGTGTTGTGT CAGCTGGAAA TCTTCTGAGT 
CATGTTGGCC ATACTATACT AGGAATGAAC ACCGTTCAAC TCTACATGAA GGTTCCAGGC 
AGTAGGACAC CAGGCCATCA AGAGAACAAC AACTTCTGTT CGGTTAATAT TAATATTGGT 
CCGGGAGATT GTGAATGGTT TGTGGTACCT GAGACGTACT GGAGCGTAAT GCATGACTTC 
TGTGAAAAGA ATAATATAAA CTTCCTTTTG GGATCATGGT GGCCCAATCT TGAAGACCTA 
TATGAAGCAA ATGTGCCAGT CTACAGGTTT ATTCAAAGGC CTGGCGACTT AGTTTGGATT 
AATGCTGGCA CAGTGCATTG GGTTCAAGCA ATTGGTTGGT GTAACAACAT TGCATGGAAT 
GTTGGCCCTC TCACAGCCTT CCAGTATAAA CTGGCAGTGG AACGGTACGA ATGGAATAAA 
TTGCAGTGCG TCAAGTCTAT TGTGCCAATG GTTCATCTCT CTTGGAATAT GGCAAAGAAT 
ATCAAAGTTT CAGATCCAAA GCTTTTTGAA ATGATTAAAT ATTGCTTATT GAGGACCTTG 
AAGCACTGTC AAGCACTGAG AGAAGTTCTT AATGCTTCTG GAATTGAAAT TGTGTGGCAT 
GGCAGAACGA AAGATGAGCC TGCTCATTAC TGTAGCAACT GTGAGGTGGA AGTCTTCAAT 
TTACTTTTTG TGACAAAAGA AAGCAATTCT CAGAAGACCT ATGTTGTTCA TTGCCATGAC 
TGCTCACGAA AATCATGCCC AAACCTAGAA AATTTTGTGG TGCTAGAACA GTTCAAAATG 
GAGGAGCTGA TGCAGATCTA TGACCTATTT ACATTAGCTC CTCCAATGCC TTCACCATCA 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751594.1
CDS	676..4761
Translation	MKSCGVSLSAAAVVAAAAASLGDEKKKMAAGKASETEEDFPNLTAEEREALVGLDSRLYGFLRLHEDGARTKALLLKAIRCYEALILKSEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFHWAIRAFQEVLYVEPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDCNPCTLSKAEIQFHIAHLYETQKKYHSAKEAYEQLLQTENLPTQVKATVLQQLGWMHHTVDQLGDKATKASYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCNNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNSAQQACKPHHQNAESVLGLSQTPISQQSLPLHMIPSSQVDELSSPAKRKRTSSPAKNTSDTWSSGHLASHTPGQQQVHSWCLPPHKVQQLEQLRANRNNLNPAQKAALEQLEAQFVLMQQQQQMRQTGLSQRNALALPHNRTNLTSSAEEPWKNQLTNSTQGLHKGQSSHLAGPNCERPPSSTGPSQHLQAAGTGIQNQVGHPTMPSNSVTQGAALNHLSSHTATSGGQQGIILTKESKPSGNGSVPETSRHSEGTPNSTAGVEGLPNHVHQVTADTVPSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNVHPAVHTKTENSVASSPSSAISTATPSPKSTEQTMTNSVVSLKSPLSGHDKVNGEQTEDSQSPTKKDHSLPSHKLSPQTIPSMSVSIYPSSVEVLKACRNLGRNGLSNSSILLDKCPPPRPPPPPYPPLPTDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHIVEVRTQLLQPADENWDPTGTKKIWRCESSRSHTTIAKYAQYQAASFQESLREENEKKSQHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDERKWKLQLHELTKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPETYWSVMHDFCEKNNINFLLGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTAFQYKLAVERYEWNKLQCVKSIVPMVHLSWNMAKNIKVSDPKLFEMIKYCLLRTLKHCQALREVLNASGIEIVWHGRTKDEPAHYCSNCEVEVFNLLFVTKESNSQKTYVVHCHDCSRKSCPNLENFVVLEQFKMEELMQIYDLFTLAPPMPSPSS

Target ORF information:

RefSeq Version	XM_031895734.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine demethylase 6A (kdm6a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895734.1

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GCTCTCCGCC GCAGCTGTTG TCGCTGCTGC TGCCGCCTCC 
CTCGGTGATG AGAAAAAGAA AATGGCGGCG GGAAAAGCGA GCGAGACCGA GGAGGACTTC 
CCCAACCTGA CAGCAGAAGA ACGAGAAGCC CTGGTCGGGC TCGACAGCCG ACTTTATGGA 
TTTTTAAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTATTAAA GGCTATTCGC 
TGTTATGAGG CTCTAATATT GAAATCTGAG GGAAAAGTGG AGTCAGATTT CTTCTGTCAG 
TTAGGTCATT TCAACCTCTT GTTGGAAGAC TATCCGAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAATCTGA CTACTGGAAG AATGCTGCCT TTCTTTATGG CCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCACTGG GCAATTAGAG CATTTCAGGA AGTGCTTTAC 
GTCGAACCCA GCTTCTGTCG CGCCAAGGAA ATTCATTTAC GACTTGGACT GATGTTTAAA 
GTGAATACTG ACTATGAGTC AAGTTTAAAG CATTTTCAGC TGGCTTTGAT TGACTGTAAT 
CCCTGCACTT TGTCCAAAGC TGAGATTCAG TTTCATATTG CTCACTTGTA CGAAACACAG 
AAAAAGTATC ACTCTGCAAA AGAAGCTTAT GAACAACTTT TGCAAACCGA AAACCTTCCA 
ACTCAAGTTA AAGCTACGGT TTTACAGCAG TTAGGTTGGA TGCATCACAC AGTTGATCAG 
CTGGGAGACA AAGCAACAAA GGCAAGCTAT GCTATTCAGT ATCTACAGAA ATCTTTAGAA 
GCAGATCCCA ACTCTGGTCA ATCCTGGTAT TTCCTTGGAA GGTGCTATTC CAGTATAGGC 
AAAGTTCAGG ATGCCTTCAT ATCCTATAGG CAGTCTATTG ATAAATCTGA GGCAAGTGCA 
GATACTTGGT GTTCTATAGG TGTGCTCTAT CAGCAGCAAA ACCAACCTAT GGATGCTCTG 
CAGGCTTACA TATGTGCTGT TCAGTTGGAC CATGGTCATG CTGCAGCATG GATGGATTTG 
GGCACATTAT ATGAATCCTG CAATCAGCCT CAGGATGCTA TCAAATGCTA CCTAAATGCA 
ACAAGGAGCA AAAATTGTAA CAATACCTCT GCACTTGCAG CAAGGATCAA ATACTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT GCTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACTCAGCAC AGCAGGCCTG TAAACCTCAT CATCAGAATG CTGAATCTGT ACTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCATTACACA TGATTCCTTC TAGCCAGGTA 
GATGAACTGT CAAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAGCAAA GAATACTTCT 
GATACCTGGA GCAGTGGCCA TTTGGCGTCA CATACTCCAG GACAGCAACA AGTACATTCG 
TGGTGTTTGC CGCCACACAA AGTACAGCAA TTGGAACAGC TACGAGCAAA CAGGAATAAT 
CTGAATCCAG CACAAAAAGC GGCGTTGGAG CAGTTGGAGG CTCAGTTTGT CTTAATGCAG 
CAGCAACAGC AGATGAGACA AACTGGACTT TCACAGCGAA ACGCACTCGC ACTACCTCAT 
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT AACTAACTCC 
ACTCAGGGGC TTCATAAAGG TCAGAGTTCA CATTTGGCAG GTCCTAATTG TGAACGACCT 
CCTTCTTCCA CTGGGCCTTC CCAGCATCTC CAAGCAGCTG GCACTGGTAT ACAAAATCAG 
GTTGGACATC CTACCATGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC 
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA TTTTAACCAA AGAGAGCAAG 
CCTTCAGGAA ATGGATCAGT GCCTGAAACA AGCAGGCATT CTGAGGGGAC ACCTAACAGC 
ACTGCCGGTG TAGAGGGACT TCCTAATCAT GTCCATCAGG TGACGGCAGA TACTGTTCCT 
AGCCCTAGCC ATGGAGATTC AAAGTCACCA GGTTTACTTA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTAGGTC CTGGAACCTG TGACAAGGTC 
AACAATGTTC ATCCAGCTGT TCATACAAAA ACTGAGAATT CTGTTGCCTC CTCTCCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCA AAATCTACCG AACAGACTAT GACCAACAGT 
GTTGTCAGCC TTAAAAGCCC TCTCAGTGGA CATGATAAAG TGAATGGAGA GCAAACGGAG 
GACTCTCAGA GCCCCACAAA AAAGGACCAC TCTTTACCTA GCCACAAATT AAGTCCACAG 
ACCATACCAT CCATGTCTGT ATCCATTTAC CCTAGCTCAG TAGAGGTTTT AAAGGCTTGT 
CGAAATCTGG GTAGAAATGG TTTATCGAAC AGTAGTATTT TACTGGACAA GTGCCCACCA 
CCAAGGCCGC CGCCTCCACC CTATCCACCT TTACCAACAG ATAAATTAAA TCCTCCAACA 
CCGAGCATTT ACTTAGAAAA CAAGAGAGAT GCTTTCTTCC CACCACTCCA TCAGTTCTGC 
ACAAATCCAA ACAACCCTGT TACTGTCATA CGAGGCCTTG CTGGAGCTCT TAAATTAGAT 
CTAGGACTAT TTTCTACAAA AACACTAGTA GAAGCGAATA ATGAACATAT AGTTGAAGTG 
AGGACACAGT TATTACAGCC TGCAGACGAA AACTGGGACC CCACTGGAAC AAAGAAAATC 
TGGCGCTGTG AAAGCAGTCG TTCTCATACC ACCATTGCAA AATATGCACA GTATCAGGCC 
GCTTCATTCC AAGAATCATT AAGAGAGGAA AATGAGAAGA AAAGCCAACA CAAAGACCAT 
TCAGATAGTG AATCCACATC TTCAGATAAC TCTGGTAGAA GACGTAAAGG CCCTTTTAAA 
ACCATTAAAT TTGGGACCAA TATTGATTTG TCTGATGAAA GAAAGTGGAA ACTACAGCTT 
CATGAACTGA CCAAACTTCC TGCGTTTGCA CGTGTTGTGT CAGCTGGAAA TCTTCTGAGT 
CATGTTGGCC ATACTATACT AGGAATGAAC ACCGTTCAAC TCTACATGAA GGTTCCAGGC 
AGTAGGACAC CAGGCCATCA AGAGAACAAC AACTTCTGTT CGGTTAATAT TAATATTGGT 
CCGGGAGATT GTGAATGGTT TGTGGTACCT GAGACGTACT GGAGCGTAAT GCATGACTTC 
TGTGAAAAGA ATAATATAAA CTTCCTTTTG GGATCATGGT GGCCCAATCT TGAAGACCTA 
TATGAAGCAA ATGTGCCAGT CTACAGGTTT ATTCAAAGGC CTGGCGACTT AGTTTGGATT 
AATGCTGGCA CAGTGCATTG GGTTCAAGCA ATTGGTTGGT GTAACAACAT TGCATGGAAT 
GTTGGCCCTC TCACAGCCTT CCAGTATAAA CTGGCAGTGG AACGGTACGA ATGGAATAAA 
TTGCAGTGCG TCAAGTCTAT TGTGCCAATG GTTCATCTCT CTTGGAATAT GGCAAAGAAT 
ATCAAAGTTT CAGATCCAAA GCTTTTTGAA ATGATTAAAT ATTGCTTATT GAGGACCTTG 
AAGCACTGTC AAGCACTGAG AGAAGTTCTT AATGCTTCTG GAATTGAAAT TGTGTGGCAT 
GGCAGAACGA AAGATGAGCC TGCTCATTAC TGTAGCAACT GTGAGGTGGA AGTCTTCAAT 
TTACTTTTTG TGACAAAAGA AAGCAATTCT CAGAAGACCT ATGTTGTTCA TTGCCATGAC 
TGCTCACGAA AATCATGCCC AAACCTAGAA AATTTTGTGG TGCTAGAACA GTTCAAAATG 
GAGGAGCTGA TGCAGATCTA TGACCTATTT ACATTAGCTC CTCCAATGCC TTCACCATCA 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative expression analysis of the H3K27 demethylases, JMJD3 and UTX, with the H3K27 methylase, EZH2, in Xenopus.
The International journal of developmental biology56(4)295-300(2012)
Kawaguchi A,Ochi H,Sudou N,Ogino H

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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kdm6a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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