clspn.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	clspn.S
Entrez Gene ID	398192
Full Name	claspin S homeolog
Synonyms	clspn
General protein information	Preferred Names claspin S homeolog Names claspin xClaspin
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018248110.1	XP_018103599.1	claspin isoform X2
NM_001088572.1	NP_001082041.1	claspin
XM_018248109.1	XP_018103598.1	claspin isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Claspin - checkpoint adaptor and DNA replication factor.
Smits VAJ, Cabrera E, Freire R, Gillespie DA
The FEBS journal286(3)441-455(2019 02)

SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin.
Ryu H, Yoshida MM, Sridharan V, Kumagai A, Dunphy WG, Dasso M, Azuma Y
Cell cycle (Georgetown, Tex.)14(17)2777-84(2015)

Phosphorylation of Claspin is triggered by the nucleocytoplasmic ratio at the Xenopus laevis midblastula transition.
Gotoh T, Kishimoto T, Sible JC
Developmental biology353(2)302-8(2011 May)

Drf1-dependent kinase interacts with Claspin through a conserved protein motif.
Gold DA, Dunphy WG
The Journal of biological chemistry285(17)12638-46(2010 Apr)

Claspin: timing the cell cycle arrest when the genome is damaged.
Freire R, van Vugt MA, Mamely I, Medema RH
Cell cycle (Georgetown, Tex.)5(24)2831-4(2006 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following clspn.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clspn.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf03574	NM_001088572.1 Latest version!	Xenopus laevis claspin S homeolog (clspn.S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAf21250	XM_018248109.1 Latest version!	Xenopus laevis claspin S homeolog (clspn.S), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAf21251	XM_018248110.1 Latest version!	Xenopus laevis claspin S homeolog (clspn.S), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clspn.S ( NM_001088572.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001082041.1 Xenopus laevis claspin S homeolog (clspn.S), mRNA.

Price & Availability↑

CloneID	OAf03574
Clone ID Related Accession (Same CDS sequence)	NM_001088572.1
Accession Version	NM_001088572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569081600000
Organism	Xenopus laevis(African clawed frog)
Product	claspin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF297867.2. On Jul 25, 2007 this sequence version replaced NM_001088573.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF297867.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04157050 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCGC TTTGCGAAGA AGAGCAGGTA TTTTTGGAAC CAGAAGACAT CAGTCTGAAA 
ATTGTGGAGA CTGATTCTGA CAGTGGTCAA GGCAGCTGTG AAATGGCTGA TCAGAATAAA 
TTATTGGGTT GTGTGGAGGA TAAAGATACA GATGATGAAA TCTTGGTTCG TAAAAAATCT 
AAAAAGAAGG AAGTATTGGT GGATAGTGAC AGTGACGAAG AATTGGAAAT GCGTAATTTT 
GCAGATAATG TAAAGGGGCA CTCTGATAAT GAGGAGAATG AGGAGACTAT GTCTGCTTAT 
AGAGAAAAAC CAAGAAAGAT CCGTTCAGCT GTATTGGACA GTGACAATAG TGATCATGAG 
CTTGATGTTC AAATAAGTAC AAGTCAAAAT GCAGCTGAAA TACCTGAGTC AGAACATGAT 
AGCTTGGAGA AGGAAACTCA TACTGTGAAG CCTAAAACAA GCAAGTCCTT GAAAAAACAA 
ACTGACACTA ATAAAGAGGA AATCGTGAAG AATAAATCAA AGCGCAAAAT TCCGAAAGAG 
AAGATTAAAA GGAGGACAAA ACAGAAGTCA AAAGCAGTTG CTGAAGCTAG GCCAAATTTA 
AATGACAGTG GCTGCTTACT CACAGATGGA GATCTTTTTG ACAATGGGGT GGAAAATGAG 
ATGGATTCTA ATGAAGAAGA GGATTCTCTT GAAGCTATCC GGGCAAAAAT GAAAAGCAAA 
CTGAATAGTC ATTCTGCTGA AAATTTTGAA GACTTTGAAC TTGATACTGA AGGCAATCAA 
GAATCCCCAG AAAAAAGAAA GGAACGAAAA GCTGCGCGAC TTGGTAAAGA AGCCATGAAA 
CAAATGCACA GTGAGACCCA AAGACTAATA CGTGAATCTT CTGTATCTTT ACCATATCAT 
CTACCTGAAC CAAAAACAAT CCATGATTTT TTCAAAAGGC GTCCAAGGCC TCTTTGTCAA 
GGAAATGCAA TGCAGCTTAT AAAGTCAACA AAATACCAGC CCTGCACTGA AGAGAAAAAA 
AAACCCAATG AGGAAATATG TGCTGAAGTT CCAGAGTTTG ATTATGTTTC AAAGGAAGAT 
TTAGAAATCA GTCCAGAGCA ACCTTTACTA AATACTCAGT GTTCACATGC TGCAGTCCTA 
TGTGTTGTGC AAAATGATGC TCGGACTGAG GGGTTAAGTA AATCCACAGA GGCAGTTGTG 
ACTGGTCAAA TGAATGACCA TGAGGATGCT TTCAGTGATT CAAACATTGT TCATGAACAA 
GAAACAGTTG GATTAATAAC CGTAACTGAA ACCTTTCAGA CACCCTTTAT TCCCCAACCA 
GAGAGCGTAG TATGTGAACA AATCCAGAAT GATGTAGTAG AGATGCAACG TATGCCTGAA 
CAACCCACGC ATAAACCCAA GTTATCCAAG CTTGAAAAGC TGAAAGCTCT TGGAGTGGAC 
TTGTCTATAA AACCTCGCCT TTGCCCTGAT GATGGTTCTT TTGTCAACTT GGATGAACCA 
AAGCCGAATA AAGAATTTGA AGCTTTGAAG GAGCGTTTCC TGAAGCACAC TCTGCAAAAG 
TCCAAACCCA GAACTGAGCG GAAAGTCAAT CTTAATATTA TCCGCAAGGA GACCACTGCT 
GATGGAAAAG AAGAACTAAA AGCAGACGTT GTGCCAATAG TTATGGCTAC AGAAAAACCA 
GACAAGAGCA TTTATCAAAA GCCAGGTGAG AAGCTGCAGG TATTGAAAGT CAAACTGCAG 
GAAGCAATGA AAATCCGTCG CAGTGAGGAG CGCCTGAAGC GGCAAGCCTT GTATAAGCTT 
GACAATGAAG ATGGCTTTGA AGATGATGAA GAGGAGGAAG AAATGACAGA GGAGTCTGAA 
GATGATGGGG ATGGAAATGC TGAGACTGCA GATTATCCTG GAGGGGAAGA TGAGGAAGAG 
GTTGGTGATG CTGAAGATGA CAATGATGAG GATGATACTG TAAATGATAG ATTGTTGGGA 
AATGTGCCTG AAATTGTTAT CCCACTGCCG AGACCAGTAA CTACTGATTC TAGCCTCATG 
CTGTTCAAGG ACAATTCTTC AAAGCTAGGA GATTCGCTAC CTGATGAAAG TGGATGCAAG 
AGAAGCAGCA GGCTAGAATA TGAAGAAGAC TCCCTGTTGC CACAATTAAA AGAAAACAGC 
CATAATAGTA GCTTTGAACT TATTAGTTCA ATGATACCAT CATACCAGCC ATGTAATAAA 
ACAACTCGAG TTGTGATCAA CTCCAATAAC CTTGGCTTTC GCTCACCATC TCCGGTTCAT 
TTCAAAACAA GTTTTCTCAG CTCTGCATCA AAGAGTTCTG GCAAGATGTC TGAACCATCC 
CTTCCCGTGG AAGACTCACA GGATCTATAT AATGCTTCCC CAGAGCCCAA AGCCTCATAT 
CTCTGTGCTG GAAGAAACTC TCAATTTCAA TTTTCGTTGG AGGATGACAC CCAGAGCCAA 
CTGCTTGATG CTGATGGGTT TCTGAATGTT GGTCGCCATA AATCTAGCTC TGCCAAACAC 
AGGCTAGCTT TGGATACAAT GGACGAGAAT GCTATGGATG CCAATATGGA TGAACTACTA 
GACCTTTGCT CAGGACAGTT CAAAGAATCT CTTTCAGGCA CATCACAGGC AGCTGAAAGT 
GATGCTAAGA AACAACCAAT GGATGAATTG CTTGAATTGT GTTCTGGCAA ATTTGTATCT 
CAAGCTGACT GCTCCACACA AGATTCTTCT GCTTCAGCTA AGGACCGTTC TACAGCTGTA 
AAAAAGGACA TTTCTGATGA AGTGGCAACG GTTTCAAGTT CATTCCTTAC TGAGAGAGAA 
CAGGAAGAAG ATGAGGAAGA AGAATTTGGT GAATTCAAGC TCTTACCCTG TGATGATTCG 
GAGAGCGAAA ACGAAGAACA AAATGAAGAG GAAGAAGAAG AAGAGGATGC TAAGGATGAT 
GAAGATGAGG AAGAAATTTT GCAGAAGCAG CAAAAGAGAA AATTGAGGCT GAATGACTTC 
ATGGAAGATG AAGCCGAATT GTCTGGAAGT GATGTAGGTA GCGGAGATGA GTATGAAGGA 
GATGATGATG AGTATGAGGA AGAAGCCATA GATGAAGATC TCCCATCTGA TGAGGAACTG 
CAGGATCAAG TCAATAAAAT TCATATGAAA GTTACCATGG ATGAAGACCA GCGACAGCTT 
CGTTTCTATC AGGAGCGGTA CCTGGCTGAT GGGGATCTCC ATAGTGACGG ACCAGGGAGA 
ACAAGAAAGT TCAGATGGAA ACATCTTGAT GATGCCTCAC AGGTGGACAT GTTCCGCCGA 
GACTCTGAAT TGGAAGAGGT GGACGGAGAG AATGAGGAAA CTGAGGAAAC CGAACTTAAA 
TGGAGGAAAG AGCGGTTCGA AAGGGAACAA TGGCTGCGAG AACAGCCACA AGGTAGTAGA 
GATAACAATG AGGAGGAGGA GGAGGATATT GGAGAAGACA GCCAGTTTAT GAAATTGGCA 
AAGAAGGTCA CTGCAAAAGC CCTACAGAGA AAAGTGAGTA CAGAGACTAA TGAACCAAAG 
AAACCTGGGC CTAGAAATCC ATATGAAGTG ATCAGGCCTT TCAGCCTCCC CAAGTTGCGT 
ACTGGTTCGC TGTTAAGCAA ACCAAAAGAA GTTTTACAGA AGCTGGCAGC TGTGTCAGAC 
CTGAATCCAA ATGCACCTCG AAACTCAAGA AACTTTGTCT TCCAAACTGT CTCACCCGGA 
AAGAAAGAAG AAACTACAGA CAAGCCAAGA TCAAAGGTAC GAAAGAATAT AGCTGTTGCC 
ATGCCTTCCC CTAAACGTTT TAAAAGGGAC AGCACCCCTA CTGTTAAAAG CCGCAGTATA 
TTTCAGTTGT TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082041.1
CDS	71..3928
Translation	MAALCEEEQVFLEPEDISLKIVETDSDSGQGSCEMADQNKLLGCVEDKDTDDEILVRKKSKKKEVLVDSDSDEELEMRNFADNVKGHSDNEENEETMSAYREKPRKIRSAVLDSDNSDHELDVQISTSQNAAEIPESEHDSLEKETHTVKPKTSKSLKKQTDTNKEEIVKNKSKRKIPKEKIKRRTKQKSKAVAEARPNLNDSGCLLTDGDLFDNGVENEMDSNEEEDSLEAIRAKMKSKLNSHSAENFEDFELDTEGNQESPEKRKERKAARLGKEAMKQMHSETQRLIRESSVSLPYHLPEPKTIHDFFKRRPRPLCQGNAMQLIKSTKYQPCTEEKKKPNEEICAEVPEFDYVSKEDLEISPEQPLLNTQCSHAAVLCVVQNDARTEGLSKSTEAVVTGQMNDHEDAFSDSNIVHEQETVGLITVTETFQTPFIPQPESVVCEQIQNDVVEMQRMPEQPTHKPKLSKLEKLKALGVDLSIKPRLCPDDGSFVNLDEPKPNKEFEALKERFLKHTLQKSKPRTERKVNLNIIRKETTADGKEELKADVVPIVMATEKPDKSIYQKPGEKLQVLKVKLQEAMKIRRSEERLKRQALYKLDNEDGFEDDEEEEEMTEESEDDGDGNAETADYPGGEDEEEVGDAEDDNDEDDTVNDRLLGNVPEIVIPLPRPVTTDSSLMLFKDNSSKLGDSLPDESGCKRSSRLEYEEDSLLPQLKENSHNSSFELISSMIPSYQPCNKTTRVVINSNNLGFRSPSPVHFKTSFLSSASKSSGKMSEPSLPVEDSQDLYNASPEPKASYLCAGRNSQFQFSLEDDTQSQLLDADGFLNVGRHKSSSAKHRLALDTMDENAMDANMDELLDLCSGQFKESLSGTSQAAESDAKKQPMDELLELCSGKFVSQADCSTQDSSASAKDRSTAVKKDISDEVATVSSSFLTEREQEEDEEEEFGEFKLLPCDDSESENEEQNEEEEEEEDAKDDEDEEEILQKQQKRKLRLNDFMEDEAELSGSDVGSGDEYEGDDDEYEEEAIDEDLPSDEELQDQVNKIHMKVTMDEDQRQLRFYQERYLADGDLHSDGPGRTRKFRWKHLDDASQVDMFRRDSELEEVDGENEETEETELKWRKERFEREQWLREQPQGSRDNNEEEEEDIGEDSQFMKLAKKVTAKALQRKVSTETNEPKKPGPRNPYEVIRPFSLPKLRTGSLLSKPKEVLQKLAAVSDLNPNAPRNSRNFVFQTVSPGKKEETTDKPRSKVRKNIAVAMPSPKRFKRDSTPTVKSRSIFQLLE

Target ORF information:

RefSeq Version	NM_001088572.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis claspin S homeolog (clspn.S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001088572.1

ORF Insert Sequence:

ATGGCCGCGC TTTGCGAAGA AGAGCAGGTA TTTTTGGAAC CAGAAGACAT CAGTCTGAAA 
ATTGTGGAGA CTGATTCTGA CAGTGGTCAA GGCAGCTGTG AAATGGCTGA TCAGAATAAA 
TTATTGGGTT GTGTGGAGGA TAAAGATACA GATGATGAAA TCTTGGTTCG TAAAAAATCT 
AAAAAGAAGG AAGTATTGGT GGATAGTGAC AGTGACGAAG AATTGGAAAT GCGTAATTTT 
GCAGATAATG TAAAGGGGCA CTCTGATAAT GAGGAGAATG AGGAGACTAT GTCTGCTTAT 
AGAGAAAAAC CAAGAAAGAT CCGTTCAGCT GTATTGGACA GTGACAATAG TGATCATGAG 
CTTGATGTTC AAATAAGTAC AAGTCAAAAT GCAGCTGAAA TACCTGAGTC AGAACATGAT 
AGCTTGGAGA AGGAAACTCA TACTGTGAAG CCTAAAACAA GCAAGTCCTT GAAAAAACAA 
ACTGACACTA ATAAAGAGGA AATCGTGAAG AATAAATCAA AGCGCAAAAT TCCGAAAGAG 
AAGATTAAAA GGAGGACAAA ACAGAAGTCA AAAGCAGTTG CTGAAGCTAG GCCAAATTTA 
AATGACAGTG GCTGCTTACT CACAGATGGA GATCTTTTTG ACAATGGGGT GGAAAATGAG 
ATGGATTCTA ATGAAGAAGA GGATTCTCTT GAAGCTATCC GGGCAAAAAT GAAAAGCAAA 
CTGAATAGTC ATTCTGCTGA AAATTTTGAA GACTTTGAAC TTGATACTGA AGGCAATCAA 
GAATCCCCAG AAAAAAGAAA GGAACGAAAA GCTGCGCGAC TTGGTAAAGA AGCCATGAAA 
CAAATGCACA GTGAGACCCA AAGACTAATA CGTGAATCTT CTGTATCTTT ACCATATCAT 
CTACCTGAAC CAAAAACAAT CCATGATTTT TTCAAAAGGC GTCCAAGGCC TCTTTGTCAA 
GGAAATGCAA TGCAGCTTAT AAAGTCAACA AAATACCAGC CCTGCACTGA AGAGAAAAAA 
AAACCCAATG AGGAAATATG TGCTGAAGTT CCAGAGTTTG ATTATGTTTC AAAGGAAGAT 
TTAGAAATCA GTCCAGAGCA ACCTTTACTA AATACTCAGT GTTCACATGC TGCAGTCCTA 
TGTGTTGTGC AAAATGATGC TCGGACTGAG GGGTTAAGTA AATCCACAGA GGCAGTTGTG 
ACTGGTCAAA TGAATGACCA TGAGGATGCT TTCAGTGATT CAAACATTGT TCATGAACAA 
GAAACAGTTG GATTAATAAC CGTAACTGAA ACCTTTCAGA CACCCTTTAT TCCCCAACCA 
GAGAGCGTAG TATGTGAACA AATCCAGAAT GATGTAGTAG AGATGCAACG TATGCCTGAA 
CAACCCACGC ATAAACCCAA GTTATCCAAG CTTGAAAAGC TGAAAGCTCT TGGAGTGGAC 
TTGTCTATAA AACCTCGCCT TTGCCCTGAT GATGGTTCTT TTGTCAACTT GGATGAACCA 
AAGCCGAATA AAGAATTTGA AGCTTTGAAG GAGCGTTTCC TGAAGCACAC TCTGCAAAAG 
TCCAAACCCA GAACTGAGCG GAAAGTCAAT CTTAATATTA TCCGCAAGGA GACCACTGCT 
GATGGAAAAG AAGAACTAAA AGCAGACGTT GTGCCAATAG TTATGGCTAC AGAAAAACCA 
GACAAGAGCA TTTATCAAAA GCCAGGTGAG AAGCTGCAGG TATTGAAAGT CAAACTGCAG 
GAAGCAATGA AAATCCGTCG CAGTGAGGAG CGCCTGAAGC GGCAAGCCTT GTATAAGCTT 
GACAATGAAG ATGGCTTTGA AGATGATGAA GAGGAGGAAG AAATGACAGA GGAGTCTGAA 
GATGATGGGG ATGGAAATGC TGAGACTGCA GATTATCCTG GAGGGGAAGA TGAGGAAGAG 
GTTGGTGATG CTGAAGATGA CAATGATGAG GATGATACTG TAAATGATAG ATTGTTGGGA 
AATGTGCCTG AAATTGTTAT CCCACTGCCG AGACCAGTAA CTACTGATTC TAGCCTCATG 
CTGTTCAAGG ACAATTCTTC AAAGCTAGGA GATTCGCTAC CTGATGAAAG TGGATGCAAG 
AGAAGCAGCA GGCTAGAATA TGAAGAAGAC TCCCTGTTGC CACAATTAAA AGAAAACAGC 
CATAATAGTA GCTTTGAACT TATTAGTTCA ATGATACCAT CATACCAGCC ATGTAATAAA 
ACAACTCGAG TTGTGATCAA CTCCAATAAC CTTGGCTTTC GCTCACCATC TCCGGTTCAT 
TTCAAAACAA GTTTTCTCAG CTCTGCATCA AAGAGTTCTG GCAAGATGTC TGAACCATCC 
CTTCCCGTGG AAGACTCACA GGATCTATAT AATGCTTCCC CAGAGCCCAA AGCCTCATAT 
CTCTGTGCTG GAAGAAACTC TCAATTTCAA TTTTCGTTGG AGGATGACAC CCAGAGCCAA 
CTGCTTGATG CTGATGGGTT TCTGAATGTT GGTCGCCATA AATCTAGCTC TGCCAAACAC 
AGGCTAGCTT TGGATACAAT GGACGAGAAT GCTATGGATG CCAATATGGA TGAACTACTA 
GACCTTTGCT CAGGACAGTT CAAAGAATCT CTTTCAGGCA CATCACAGGC AGCTGAAAGT 
GATGCTAAGA AACAACCAAT GGATGAATTG CTTGAATTGT GTTCTGGCAA ATTTGTATCT 
CAAGCTGACT GCTCCACACA AGATTCTTCT GCTTCAGCTA AGGACCGTTC TACAGCTGTA 
AAAAAGGACA TTTCTGATGA AGTGGCAACG GTTTCAAGTT CATTCCTTAC TGAGAGAGAA 
CAGGAAGAAG ATGAGGAAGA AGAATTTGGT GAATTCAAGC TCTTACCCTG TGATGATTCG 
GAGAGCGAAA ACGAAGAACA AAATGAAGAG GAAGAAGAAG AAGAGGATGC TAAGGATGAT 
GAAGATGAGG AAGAAATTTT GCAGAAGCAG CAAAAGAGAA AATTGAGGCT GAATGACTTC 
ATGGAAGATG AAGCCGAATT GTCTGGAAGT GATGTAGGTA GCGGAGATGA GTATGAAGGA 
GATGATGATG AGTATGAGGA AGAAGCCATA GATGAAGATC TCCCATCTGA TGAGGAACTG 
CAGGATCAAG TCAATAAAAT TCATATGAAA GTTACCATGG ATGAAGACCA GCGACAGCTT 
CGTTTCTATC AGGAGCGGTA CCTGGCTGAT GGGGATCTCC ATAGTGACGG ACCAGGGAGA 
ACAAGAAAGT TCAGATGGAA ACATCTTGAT GATGCCTCAC AGGTGGACAT GTTCCGCCGA 
GACTCTGAAT TGGAAGAGGT GGACGGAGAG AATGAGGAAA CTGAGGAAAC CGAACTTAAA 
TGGAGGAAAG AGCGGTTCGA AAGGGAACAA TGGCTGCGAG AACAGCCACA AGGTAGTAGA 
GATAACAATG AGGAGGAGGA GGAGGATATT GGAGAAGACA GCCAGTTTAT GAAATTGGCA 
AAGAAGGTCA CTGCAAAAGC CCTACAGAGA AAAGTGAGTA CAGAGACTAA TGAACCAAAG 
AAACCTGGGC CTAGAAATCC ATATGAAGTG ATCAGGCCTT TCAGCCTCCC CAAGTTGCGT 
ACTGGTTCGC TGTTAAGCAA ACCAAAAGAA GTTTTACAGA AGCTGGCAGC TGTGTCAGAC 
CTGAATCCAA ATGCACCTCG AAACTCAAGA AACTTTGTCT TCCAAACTGT CTCACCCGGA 
AAGAAAGAAG AAACTACAGA CAAGCCAAGA TCAAAGGTAC GAAAGAATAT AGCTGTTGCC 
ATGCCTTCCC CTAAACGTTT TAAAAGGGAC AGCACCCCTA CTGTTAAAAG CCGCAGTATA 
TTTCAGTTGT TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clspn.S ( XM_018248109.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018103598.1 Xenopus laevis claspin S homeolog (clspn.S), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf21250
Clone ID Related Accession (Same CDS sequence)	XM_018248109.1
Accession Version	XM_018248109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	claspin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAGG CACTGGCTCA CTTTGTACCT ATACTCCGGC GCAAAACGGT ATTTTTGGAA 
CCAGAAGACA TCAGTCTGAA AATTGTGGAG ACTGATTCTG ACAGTGGTCA AGGCAGCTGT 
GAAATGGCTG ATCAGAATAA ATTATTGGGT TGTGTGGAGG ATAAAGATAC AGATGATGAA 
ATCTTGGTTC GTAAAAAATC TAAAAAGAAG AAAGTATTGG TGGATAGTGA CAGTGACGAA 
GAATTGGAAA TGCGTAATTT TGCAGATAAT GTAAAGGGGC ACTCTGATAA TGAGGAGAAT 
GAGGAGACTA TGTCTGCTTA TAGAGAAAAA CCAAGAAAGA TCCGTTCAGC TGTATTGGAC 
AGTGACAATA GTGATCATGA GCTTGATGTT CAAATAAGTA CAAGTCAAAA TGCAGCTGAA 
ATACCTGAGT CAGAACATGG TAGCTTGGAG AAGGAAACTC ATACTGTGAA GCCTAAAACA 
AGCAAGTCCT TGAAAAAACA AACTGACACT AATAAAGAGG AAATCGTGAA GAATAAATCA 
AAGCGCAAAA TTCCGAAAGA GGAGAAGATT AAAAGGAGGA CAAAACAGAA GTCAAAAGCA 
GTTGCTGAAG CTAGGCCAAA TTTAAATGAC AGTGGCTGCC TACTCACAGA TGGAGATCTT 
TTTGACAATG GGGTGGAAAA TGAGATGGAT TCTAATGAAG AAGAGGATTC TCTTGAAGCT 
ATCCGGGCAA AAATGAAAAG CAAACTGAAT AGTCATTCTG CTGAAAATTT TGAAGACTTT 
GAACTTGATA CTGAAGGCAA TCAAGAATCC CCAGAAAAAA GAAAGGAACG AAAAGCTGCG 
CGACTTGGTA AAGAAGCCAT GAAACAAATG CACAGTGAGA CCCAAAGACT AATACGTGAA 
TCTTCTGTAT CTTTACCATA TCATCTACCT GAACCAAAAA CAATCCATGA TTTTTTCAAA 
AGGCGTCCAA GGCCTCTTTG TCAAGGAAAT GCAATGCAGC TTATAAAGTC AACAAAATAC 
CAGCCCTGCA CTGAAGAGAA AAAAAAACCC AATGAGGAAA TATGTGCTGA AGTTCCAGAG 
TTTGATTATG TTTCAAAGGA AGATTTAGAA ATCAGTCCAG AGCAACCTTT ACTAAATACT 
CAGTGTTCAC ATGCTGCAGT CCTATGTGTT GTGCAAAATG ATGCTCGGAC TGAGGGGTTA 
AGTAAATCCA CAGAGGCAGT TGTGACTGGT CAAATGAATG ACCATGAGGA TGCTTTCAGT 
GATTCAAACA TTGTTCATGA ACAAGAAACA GTTGGATTAA TAACCGTAAC TGAAACCTTT 
CAGACACCCT TTATTCCCCA ACCAGAGAGC GTAGTATGTG AACAAATCCA GAATGATGTA 
GTAGAGATGC AACGTATGCC TGAACAACCC ACGCATAAAC CCAAGTTATC CAAGCTTGAA 
AAGCTGAAAG CTCTTGGAGT GGACTTGTCT ATAAAACCTC GCCTTTGCCC TGATGATGGT 
TCTTTTGTCA ACTTGGATGA ACCAAAGCCG AATAAAGAAT TTGAAGCTTT GAAGGAGCGT 
TTCCTGAAGC ACACTCTGCA AAAGTCCAAA CCCAGAACTG AGCGGAAAGT CAATCTTAAT 
ATTATCCGCA AGGAGACCAC TGCTGATGGA AAAGAAGAAC TAAAAGCAGA CGTTGTGCCA 
ATAGTTATGG CTACAGAAAA ACCAGACAAG AGCATTTATC AAAAGCCAGG TGAGAAGCTG 
CAGGTATTGA AAGTCAAACT GCAGGAAGCA ATGAAAATCC GTCGCAGTGA GGAGCGCCTG 
AAGCGGCAAG CCTTGTATAA GCTTGACAAT GAAGATGGCT TTGAAGATGA TGAAGAGGAG 
GAAGAAATGA CAGAGGAGTC TGAAGATGAT GGGGATGGAA ATGCTGAGAC TGCAGATTAT 
CCTGGAGGGG AAGATGAGGA AGAGGTTGGT GATGCTGAAG ATGACAATGA TGAGGATGAT 
ACTGTAAATG ATAGATTGTT GGGAAATGTG CCTGAAATTG TTATCCCACT GCCGAGACCA 
GTAACTACTG ATTCTAGCCT CATGCTGTTC AAGGACAATT CTTCAAAGCT AGGAGATTCG 
CTACCTGATG AAAGTGGATG CAAGAGAAGC AGCAGGCTAG AATATGAAGA AGACTCCCTG 
TTGCCACAAT TAAAAGAAAA CAGCCATAAT AGTAGCTTTG AACTTATTAG TTCAATGATA 
CCATCATACC AGCCATGTAA TAAAACAACT CGAGTTGTGA TCAACTCCAA TAACCTTGGC 
TTTCGCTCAC CATCTCCGGT TCATTTCAAA ACAAGTTTTC TCAGCTCTGC ATCAAAGAGT 
TCTGGCAAGA TGTCTGAACC ATCCCTTCCC GTGGAAGACT CACAGGATCT ATATAATGCT 
TCCCCAGAGC CCAAAGCCTC ATATCTCTGT GCTGGAAGAA ACTCTCAATT TCAATTTTCG 
TTGGAGGATG ACACCCAGAG CCAACTGCTT GATGCTGATG GGTTTCTGAA TGTTGGTCGC 
CATAAATCTA GCTCTGCCAA ACACAGGCTA GCTTTGGATA CAATGGACGA GAATGCTATG 
GATGCCAATA TGGATGAACT ACTAGACCTT TGCTCAGGAC AGTTCAAAGA ATCTCTTTCA 
GGCACATCAC AGGCAGCTGA AAGTGATGCT AAGAAACAAC CAATGGATGA ATTGCTTGAA 
TTGTGTTCTG GCAAATTTGT ATCTCAAGCT GACTGCTCCA CACAAGATTC TTCTGCTTCA 
GCTAAGGACC GTTCTACAGC TGTAAAAAAG GACATTTCTG ATGAAGTGGC AACGGTTTCA 
AGTTCATTCC TTACTGAGAG AGAACAGGAA GAAGATGAGG AAGAAGAATT TGGTGAATTC 
AAGCTCTTAC CCTGTGATGA TTCGGAGAGC GAAAACGAAG AACAAAATGA AGAGGAAGAA 
GAAGAAGAGG ATGCTAAGGA TGATGAAGAT GAGGAAGAAA TTTTGCAGAA GCAGCAAAAG 
AGAAAATTGA GGCTGAATGA CTTCATGGAA GATGAAGCCG AATTGTCTGG AAGTGATGTA 
GGTAGCGGAG ATGAGTATGA AGGAGATGAT GATGAGTATG AGGAAGAAGC CATAGATGAA 
GATCTCCCAT CTGATGAGGA ACTGCAGGAT CAAGTCAATA AAATTCATAT GAAAGTTACC 
ATGGATGAAG ACCAGCGACA GCTTCGTTTC TATCAGGAGC GGTACCTGGC TGATGGGGAT 
CTCCATAGTG ACGGACCAGG GAGAACAAGA AAGTTCAGAT GGAAACATCT TGATGATGCC 
TCACAGGTGG ACATGTTCCG CCGAGACTCT GAATTGGAAG AGGTGGACGG AGAGAATGAG 
GAAACTGAGG AAACCGAACT TAAATGGAGG AAAGAGCGGT TCGAAAGGGA ACAATGGCTG 
CGAGAACAGC CACAAGGTAG TAGAGATAAC AATGAGGAGG AGGAGGAGGA TATTGGAGAA 
GACAGCCAGT TTATGAAATT GGCAAAGAAG GTCACTGCAA AAGCCCTACA GAGAAAAGTG 
AGTACAGAGA CTAATGAACC AAAGAAACCT GGGCCTAGAA ATCCATATGA AGTGATCAGG 
CCTTTCAGCC TCCCCAAGTT GCGTACTGGT TCGCTGTTAA GCAAACCAAA AGAAGTTTTA 
CAGAAGCTGG CAGCTGTGTC AGACCTGAAT CCAAATGCAC CTCGAAACTC AAGAAACTTT 
GTCTTCCAAA CTGTCTCACC CGGAAAGAAA GAAGAAACTA CAGACAAGCC AAGATCAAAG 
GTACGAAAGA ATATAGCTGT TGCCATGCCT TCCCCTAAAC GTTTTAAAAG GGACAGCACC 
CCTACTGTTA AAAGCCGCAG TATATTTCAG TTGTTGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018103598.1
CDS	127..4008
Translation	MHKALAHFVPILRRKTVFLEPEDISLKIVETDSDSGQGSCEMADQNKLLGCVEDKDTDDEILVRKKSKKKKVLVDSDSDEELEMRNFADNVKGHSDNEENEETMSAYREKPRKIRSAVLDSDNSDHELDVQISTSQNAAEIPESEHGSLEKETHTVKPKTSKSLKKQTDTNKEEIVKNKSKRKIPKEEKIKRRTKQKSKAVAEARPNLNDSGCLLTDGDLFDNGVENEMDSNEEEDSLEAIRAKMKSKLNSHSAENFEDFELDTEGNQESPEKRKERKAARLGKEAMKQMHSETQRLIRESSVSLPYHLPEPKTIHDFFKRRPRPLCQGNAMQLIKSTKYQPCTEEKKKPNEEICAEVPEFDYVSKEDLEISPEQPLLNTQCSHAAVLCVVQNDARTEGLSKSTEAVVTGQMNDHEDAFSDSNIVHEQETVGLITVTETFQTPFIPQPESVVCEQIQNDVVEMQRMPEQPTHKPKLSKLEKLKALGVDLSIKPRLCPDDGSFVNLDEPKPNKEFEALKERFLKHTLQKSKPRTERKVNLNIIRKETTADGKEELKADVVPIVMATEKPDKSIYQKPGEKLQVLKVKLQEAMKIRRSEERLKRQALYKLDNEDGFEDDEEEEEMTEESEDDGDGNAETADYPGGEDEEEVGDAEDDNDEDDTVNDRLLGNVPEIVIPLPRPVTTDSSLMLFKDNSSKLGDSLPDESGCKRSSRLEYEEDSLLPQLKENSHNSSFELISSMIPSYQPCNKTTRVVINSNNLGFRSPSPVHFKTSFLSSASKSSGKMSEPSLPVEDSQDLYNASPEPKASYLCAGRNSQFQFSLEDDTQSQLLDADGFLNVGRHKSSSAKHRLALDTMDENAMDANMDELLDLCSGQFKESLSGTSQAAESDAKKQPMDELLELCSGKFVSQADCSTQDSSASAKDRSTAVKKDISDEVATVSSSFLTEREQEEDEEEEFGEFKLLPCDDSESENEEQNEEEEEEEDAKDDEDEEEILQKQQKRKLRLNDFMEDEAELSGSDVGSGDEYEGDDDEYEEEAIDEDLPSDEELQDQVNKIHMKVTMDEDQRQLRFYQERYLADGDLHSDGPGRTRKFRWKHLDDASQVDMFRRDSELEEVDGENEETEETELKWRKERFEREQWLREQPQGSRDNNEEEEEDIGEDSQFMKLAKKVTAKALQRKVSTETNEPKKPGPRNPYEVIRPFSLPKLRTGSLLSKPKEVLQKLAAVSDLNPNAPRNSRNFVFQTVSPGKKEETTDKPRSKVRKNIAVAMPSPKRFKRDSTPTVKSRSIFQLLE

Target ORF information:

RefSeq Version	XM_018248109.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis claspin S homeolog (clspn.S), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018248109.1

ORF Insert Sequence:

ATGCATAAGG CACTGGCTCA CTTTGTACCT ATACTCCGGC GCAAAACGGT ATTTTTGGAA 
CCAGAAGACA TCAGTCTGAA AATTGTGGAG ACTGATTCTG ACAGTGGTCA AGGCAGCTGT 
GAAATGGCTG ATCAGAATAA ATTATTGGGT TGTGTGGAGG ATAAAGATAC AGATGATGAA 
ATCTTGGTTC GTAAAAAATC TAAAAAGAAG AAAGTATTGG TGGATAGTGA CAGTGACGAA 
GAATTGGAAA TGCGTAATTT TGCAGATAAT GTAAAGGGGC ACTCTGATAA TGAGGAGAAT 
GAGGAGACTA TGTCTGCTTA TAGAGAAAAA CCAAGAAAGA TCCGTTCAGC TGTATTGGAC 
AGTGACAATA GTGATCATGA GCTTGATGTT CAAATAAGTA CAAGTCAAAA TGCAGCTGAA 
ATACCTGAGT CAGAACATGG TAGCTTGGAG AAGGAAACTC ATACTGTGAA GCCTAAAACA 
AGCAAGTCCT TGAAAAAACA AACTGACACT AATAAAGAGG AAATCGTGAA GAATAAATCA 
AAGCGCAAAA TTCCGAAAGA GGAGAAGATT AAAAGGAGGA CAAAACAGAA GTCAAAAGCA 
GTTGCTGAAG CTAGGCCAAA TTTAAATGAC AGTGGCTGCC TACTCACAGA TGGAGATCTT 
TTTGACAATG GGGTGGAAAA TGAGATGGAT TCTAATGAAG AAGAGGATTC TCTTGAAGCT 
ATCCGGGCAA AAATGAAAAG CAAACTGAAT AGTCATTCTG CTGAAAATTT TGAAGACTTT 
GAACTTGATA CTGAAGGCAA TCAAGAATCC CCAGAAAAAA GAAAGGAACG AAAAGCTGCG 
CGACTTGGTA AAGAAGCCAT GAAACAAATG CACAGTGAGA CCCAAAGACT AATACGTGAA 
TCTTCTGTAT CTTTACCATA TCATCTACCT GAACCAAAAA CAATCCATGA TTTTTTCAAA 
AGGCGTCCAA GGCCTCTTTG TCAAGGAAAT GCAATGCAGC TTATAAAGTC AACAAAATAC 
CAGCCCTGCA CTGAAGAGAA AAAAAAACCC AATGAGGAAA TATGTGCTGA AGTTCCAGAG 
TTTGATTATG TTTCAAAGGA AGATTTAGAA ATCAGTCCAG AGCAACCTTT ACTAAATACT 
CAGTGTTCAC ATGCTGCAGT CCTATGTGTT GTGCAAAATG ATGCTCGGAC TGAGGGGTTA 
AGTAAATCCA CAGAGGCAGT TGTGACTGGT CAAATGAATG ACCATGAGGA TGCTTTCAGT 
GATTCAAACA TTGTTCATGA ACAAGAAACA GTTGGATTAA TAACCGTAAC TGAAACCTTT 
CAGACACCCT TTATTCCCCA ACCAGAGAGC GTAGTATGTG AACAAATCCA GAATGATGTA 
GTAGAGATGC AACGTATGCC TGAACAACCC ACGCATAAAC CCAAGTTATC CAAGCTTGAA 
AAGCTGAAAG CTCTTGGAGT GGACTTGTCT ATAAAACCTC GCCTTTGCCC TGATGATGGT 
TCTTTTGTCA ACTTGGATGA ACCAAAGCCG AATAAAGAAT TTGAAGCTTT GAAGGAGCGT 
TTCCTGAAGC ACACTCTGCA AAAGTCCAAA CCCAGAACTG AGCGGAAAGT CAATCTTAAT 
ATTATCCGCA AGGAGACCAC TGCTGATGGA AAAGAAGAAC TAAAAGCAGA CGTTGTGCCA 
ATAGTTATGG CTACAGAAAA ACCAGACAAG AGCATTTATC AAAAGCCAGG TGAGAAGCTG 
CAGGTATTGA AAGTCAAACT GCAGGAAGCA ATGAAAATCC GTCGCAGTGA GGAGCGCCTG 
AAGCGGCAAG CCTTGTATAA GCTTGACAAT GAAGATGGCT TTGAAGATGA TGAAGAGGAG 
GAAGAAATGA CAGAGGAGTC TGAAGATGAT GGGGATGGAA ATGCTGAGAC TGCAGATTAT 
CCTGGAGGGG AAGATGAGGA AGAGGTTGGT GATGCTGAAG ATGACAATGA TGAGGATGAT 
ACTGTAAATG ATAGATTGTT GGGAAATGTG CCTGAAATTG TTATCCCACT GCCGAGACCA 
GTAACTACTG ATTCTAGCCT CATGCTGTTC AAGGACAATT CTTCAAAGCT AGGAGATTCG 
CTACCTGATG AAAGTGGATG CAAGAGAAGC AGCAGGCTAG AATATGAAGA AGACTCCCTG 
TTGCCACAAT TAAAAGAAAA CAGCCATAAT AGTAGCTTTG AACTTATTAG TTCAATGATA 
CCATCATACC AGCCATGTAA TAAAACAACT CGAGTTGTGA TCAACTCCAA TAACCTTGGC 
TTTCGCTCAC CATCTCCGGT TCATTTCAAA ACAAGTTTTC TCAGCTCTGC ATCAAAGAGT 
TCTGGCAAGA TGTCTGAACC ATCCCTTCCC GTGGAAGACT CACAGGATCT ATATAATGCT 
TCCCCAGAGC CCAAAGCCTC ATATCTCTGT GCTGGAAGAA ACTCTCAATT TCAATTTTCG 
TTGGAGGATG ACACCCAGAG CCAACTGCTT GATGCTGATG GGTTTCTGAA TGTTGGTCGC 
CATAAATCTA GCTCTGCCAA ACACAGGCTA GCTTTGGATA CAATGGACGA GAATGCTATG 
GATGCCAATA TGGATGAACT ACTAGACCTT TGCTCAGGAC AGTTCAAAGA ATCTCTTTCA 
GGCACATCAC AGGCAGCTGA AAGTGATGCT AAGAAACAAC CAATGGATGA ATTGCTTGAA 
TTGTGTTCTG GCAAATTTGT ATCTCAAGCT GACTGCTCCA CACAAGATTC TTCTGCTTCA 
GCTAAGGACC GTTCTACAGC TGTAAAAAAG GACATTTCTG ATGAAGTGGC AACGGTTTCA 
AGTTCATTCC TTACTGAGAG AGAACAGGAA GAAGATGAGG AAGAAGAATT TGGTGAATTC 
AAGCTCTTAC CCTGTGATGA TTCGGAGAGC GAAAACGAAG AACAAAATGA AGAGGAAGAA 
GAAGAAGAGG ATGCTAAGGA TGATGAAGAT GAGGAAGAAA TTTTGCAGAA GCAGCAAAAG 
AGAAAATTGA GGCTGAATGA CTTCATGGAA GATGAAGCCG AATTGTCTGG AAGTGATGTA 
GGTAGCGGAG ATGAGTATGA AGGAGATGAT GATGAGTATG AGGAAGAAGC CATAGATGAA 
GATCTCCCAT CTGATGAGGA ACTGCAGGAT CAAGTCAATA AAATTCATAT GAAAGTTACC 
ATGGATGAAG ACCAGCGACA GCTTCGTTTC TATCAGGAGC GGTACCTGGC TGATGGGGAT 
CTCCATAGTG ACGGACCAGG GAGAACAAGA AAGTTCAGAT GGAAACATCT TGATGATGCC 
TCACAGGTGG ACATGTTCCG CCGAGACTCT GAATTGGAAG AGGTGGACGG AGAGAATGAG 
GAAACTGAGG AAACCGAACT TAAATGGAGG AAAGAGCGGT TCGAAAGGGA ACAATGGCTG 
CGAGAACAGC CACAAGGTAG TAGAGATAAC AATGAGGAGG AGGAGGAGGA TATTGGAGAA 
GACAGCCAGT TTATGAAATT GGCAAAGAAG GTCACTGCAA AAGCCCTACA GAGAAAAGTG 
AGTACAGAGA CTAATGAACC AAAGAAACCT GGGCCTAGAA ATCCATATGA AGTGATCAGG 
CCTTTCAGCC TCCCCAAGTT GCGTACTGGT TCGCTGTTAA GCAAACCAAA AGAAGTTTTA 
CAGAAGCTGG CAGCTGTGTC AGACCTGAAT CCAAATGCAC CTCGAAACTC AAGAAACTTT 
GTCTTCCAAA CTGTCTCACC CGGAAAGAAA GAAGAAACTA CAGACAAGCC AAGATCAAAG 
GTACGAAAGA ATATAGCTGT TGCCATGCCT TCCCCTAAAC GTTTTAAAAG GGACAGCACC 
CCTACTGTTA AAAGCCGCAG TATATTTCAG TTGTTGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clspn.S ( XM_018248110.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018103599.1 Xenopus laevis claspin S homeolog (clspn.S), transcript variant X2, mRNA.

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CloneID	OAf21251
Clone ID Related Accession (Same CDS sequence)	XM_018248110.1
Accession Version	XM_018248110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	claspin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATC AGAATAAATT ATTGGGTTGT GTGGAGGATA AAGATACAGA TGATGAAATC 
TTGGTTCGTA AAAAATCTAA AAAGAAGAAA GTATTGGTGG ATAGTGACAG TGACGAAGAA 
TTGGAAATGC GTAATTTTGC AGATAATGTA AAGGGGCACT CTGATAATGA GGAGAATGAG 
GAGACTATGT CTGCTTATAG AGAAAAACCA AGAAAGATCC GTTCAGCTGT ATTGGACAGT 
GACAATAGTG ATCATGAGCT TGATGTTCAA ATAAGTACAA GTCAAAATGC AGCTGAAATA 
CCTGAGTCAG AACATGGTAG CTTGGAGAAG GAAACTCATA CTGTGAAGCC TAAAACAAGC 
AAGTCCTTGA AAAAACAAAC TGACACTAAT AAAGAGGAAA TCGTGAAGAA TAAATCAAAG 
CGCAAAATTC CGAAAGAGGA GAAGATTAAA AGGAGGACAA AACAGAAGTC AAAAGCAGTT 
GCTGAAGCTA GGCCAAATTT AAATGACAGT GGCTGCCTAC TCACAGATGG AGATCTTTTT 
GACAATGGGG TGGAAAATGA GATGGATTCT AATGAAGAAG AGGATTCTCT TGAAGCTATC 
CGGGCAAAAA TGAAAAGCAA ACTGAATAGT CATTCTGCTG AAAATTTTGA AGACTTTGAA 
CTTGATACTG AAGGCAATCA AGAATCCCCA GAAAAAAGAA AGGAACGAAA AGCTGCGCGA 
CTTGGTAAAG AAGCCATGAA ACAAATGCAC AGTGAGACCC AAAGACTAAT ACGTGAATCT 
TCTGTATCTT TACCATATCA TCTACCTGAA CCAAAAACAA TCCATGATTT TTTCAAAAGG 
CGTCCAAGGC CTCTTTGTCA AGGAAATGCA ATGCAGCTTA TAAAGTCAAC AAAATACCAG 
CCCTGCACTG AAGAGAAAAA AAAACCCAAT GAGGAAATAT GTGCTGAAGT TCCAGAGTTT 
GATTATGTTT CAAAGGAAGA TTTAGAAATC AGTCCAGAGC AACCTTTACT AAATACTCAG 
TGTTCACATG CTGCAGTCCT ATGTGTTGTG CAAAATGATG CTCGGACTGA GGGGTTAAGT 
AAATCCACAG AGGCAGTTGT GACTGGTCAA ATGAATGACC ATGAGGATGC TTTCAGTGAT 
TCAAACATTG TTCATGAACA AGAAACAGTT GGATTAATAA CCGTAACTGA AACCTTTCAG 
ACACCCTTTA TTCCCCAACC AGAGAGCGTA GTATGTGAAC AAATCCAGAA TGATGTAGTA 
GAGATGCAAC GTATGCCTGA ACAACCCACG CATAAACCCA AGTTATCCAA GCTTGAAAAG 
CTGAAAGCTC TTGGAGTGGA CTTGTCTATA AAACCTCGCC TTTGCCCTGA TGATGGTTCT 
TTTGTCAACT TGGATGAACC AAAGCCGAAT AAAGAATTTG AAGCTTTGAA GGAGCGTTTC 
CTGAAGCACA CTCTGCAAAA GTCCAAACCC AGAACTGAGC GGAAAGTCAA TCTTAATATT 
ATCCGCAAGG AGACCACTGC TGATGGAAAA GAAGAACTAA AAGCAGACGT TGTGCCAATA 
GTTATGGCTA CAGAAAAACC AGACAAGAGC ATTTATCAAA AGCCAGGTGA GAAGCTGCAG 
GTATTGAAAG TCAAACTGCA GGAAGCAATG AAAATCCGTC GCAGTGAGGA GCGCCTGAAG 
CGGCAAGCCT TGTATAAGCT TGACAATGAA GATGGCTTTG AAGATGATGA AGAGGAGGAA 
GAAATGACAG AGGAGTCTGA AGATGATGGG GATGGAAATG CTGAGACTGC AGATTATCCT 
GGAGGGGAAG ATGAGGAAGA GGTTGGTGAT GCTGAAGATG ACAATGATGA GGATGATACT 
GTAAATGATA GATTGTTGGG AAATGTGCCT GAAATTGTTA TCCCACTGCC GAGACCAGTA 
ACTACTGATT CTAGCCTCAT GCTGTTCAAG GACAATTCTT CAAAGCTAGG AGATTCGCTA 
CCTGATGAAA GTGGATGCAA GAGAAGCAGC AGGCTAGAAT ATGAAGAAGA CTCCCTGTTG 
CCACAATTAA AAGAAAACAG CCATAATAGT AGCTTTGAAC TTATTAGTTC AATGATACCA 
TCATACCAGC CATGTAATAA AACAACTCGA GTTGTGATCA ACTCCAATAA CCTTGGCTTT 
CGCTCACCAT CTCCGGTTCA TTTCAAAACA AGTTTTCTCA GCTCTGCATC AAAGAGTTCT 
GGCAAGATGT CTGAACCATC CCTTCCCGTG GAAGACTCAC AGGATCTATA TAATGCTTCC 
CCAGAGCCCA AAGCCTCATA TCTCTGTGCT GGAAGAAACT CTCAATTTCA ATTTTCGTTG 
GAGGATGACA CCCAGAGCCA ACTGCTTGAT GCTGATGGGT TTCTGAATGT TGGTCGCCAT 
AAATCTAGCT CTGCCAAACA CAGGCTAGCT TTGGATACAA TGGACGAGAA TGCTATGGAT 
GCCAATATGG ATGAACTACT AGACCTTTGC TCAGGACAGT TCAAAGAATC TCTTTCAGGC 
ACATCACAGG CAGCTGAAAG TGATGCTAAG AAACAACCAA TGGATGAATT GCTTGAATTG 
TGTTCTGGCA AATTTGTATC TCAAGCTGAC TGCTCCACAC AAGATTCTTC TGCTTCAGCT 
AAGGACCGTT CTACAGCTGT AAAAAAGGAC ATTTCTGATG AAGTGGCAAC GGTTTCAAGT 
TCATTCCTTA CTGAGAGAGA ACAGGAAGAA GATGAGGAAG AAGAATTTGG TGAATTCAAG 
CTCTTACCCT GTGATGATTC GGAGAGCGAA AACGAAGAAC AAAATGAAGA GGAAGAAGAA 
GAAGAGGATG CTAAGGATGA TGAAGATGAG GAAGAAATTT TGCAGAAGCA GCAAAAGAGA 
AAATTGAGGC TGAATGACTT CATGGAAGAT GAAGCCGAAT TGTCTGGAAG TGATGTAGGT 
AGCGGAGATG AGTATGAAGG AGATGATGAT GAGTATGAGG AAGAAGCCAT AGATGAAGAT 
CTCCCATCTG ATGAGGAACT GCAGGATCAA GTCAATAAAA TTCATATGAA AGTTACCATG 
GATGAAGACC AGCGACAGCT TCGTTTCTAT CAGGAGCGGT ACCTGGCTGA TGGGGATCTC 
CATAGTGACG GACCAGGGAG AACAAGAAAG TTCAGATGGA AACATCTTGA TGATGCCTCA 
CAGGTGGACA TGTTCCGCCG AGACTCTGAA TTGGAAGAGG TGGACGGAGA GAATGAGGAA 
ACTGAGGAAA CCGAACTTAA ATGGAGGAAA GAGCGGTTCG AAAGGGAACA ATGGCTGCGA 
GAACAGCCAC AAGGTAGTAG AGATAACAAT GAGGAGGAGG AGGAGGATAT TGGAGAAGAC 
AGCCAGTTTA TGAAATTGGC AAAGAAGGTC ACTGCAAAAG CCCTACAGAG AAAAGTGAGT 
ACAGAGACTA ATGAACCAAA GAAACCTGGG CCTAGAAATC CATATGAAGT GATCAGGCCT 
TTCAGCCTCC CCAAGTTGCG TACTGGTTCG CTGTTAAGCA AACCAAAAGA AGTTTTACAG 
AAGCTGGCAG CTGTGTCAGA CCTGAATCCA AATGCACCTC GAAACTCAAG AAACTTTGTC 
TTCCAAACTG TCTCACCCGG AAAGAAAGAA GAAACTACAG ACAAGCCAAG ATCAAAGGTA 
CGAAAGAATA TAGCTGTTGC CATGCCTTCC CCTAAACGTT TTAAAAGGGA CAGCACCCCT 
ACTGTTAAAA GCCGCAGTAT ATTTCAGTTG TTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018103599.1
CDS	162..3920
Translation	MADQNKLLGCVEDKDTDDEILVRKKSKKKKVLVDSDSDEELEMRNFADNVKGHSDNEENEETMSAYREKPRKIRSAVLDSDNSDHELDVQISTSQNAAEIPESEHGSLEKETHTVKPKTSKSLKKQTDTNKEEIVKNKSKRKIPKEEKIKRRTKQKSKAVAEARPNLNDSGCLLTDGDLFDNGVENEMDSNEEEDSLEAIRAKMKSKLNSHSAENFEDFELDTEGNQESPEKRKERKAARLGKEAMKQMHSETQRLIRESSVSLPYHLPEPKTIHDFFKRRPRPLCQGNAMQLIKSTKYQPCTEEKKKPNEEICAEVPEFDYVSKEDLEISPEQPLLNTQCSHAAVLCVVQNDARTEGLSKSTEAVVTGQMNDHEDAFSDSNIVHEQETVGLITVTETFQTPFIPQPESVVCEQIQNDVVEMQRMPEQPTHKPKLSKLEKLKALGVDLSIKPRLCPDDGSFVNLDEPKPNKEFEALKERFLKHTLQKSKPRTERKVNLNIIRKETTADGKEELKADVVPIVMATEKPDKSIYQKPGEKLQVLKVKLQEAMKIRRSEERLKRQALYKLDNEDGFEDDEEEEEMTEESEDDGDGNAETADYPGGEDEEEVGDAEDDNDEDDTVNDRLLGNVPEIVIPLPRPVTTDSSLMLFKDNSSKLGDSLPDESGCKRSSRLEYEEDSLLPQLKENSHNSSFELISSMIPSYQPCNKTTRVVINSNNLGFRSPSPVHFKTSFLSSASKSSGKMSEPSLPVEDSQDLYNASPEPKASYLCAGRNSQFQFSLEDDTQSQLLDADGFLNVGRHKSSSAKHRLALDTMDENAMDANMDELLDLCSGQFKESLSGTSQAAESDAKKQPMDELLELCSGKFVSQADCSTQDSSASAKDRSTAVKKDISDEVATVSSSFLTEREQEEDEEEEFGEFKLLPCDDSESENEEQNEEEEEEEDAKDDEDEEEILQKQQKRKLRLNDFMEDEAELSGSDVGSGDEYEGDDDEYEEEAIDEDLPSDEELQDQVNKIHMKVTMDEDQRQLRFYQERYLADGDLHSDGPGRTRKFRWKHLDDASQVDMFRRDSELEEVDGENEETEETELKWRKERFEREQWLREQPQGSRDNNEEEEEDIGEDSQFMKLAKKVTAKALQRKVSTETNEPKKPGPRNPYEVIRPFSLPKLRTGSLLSKPKEVLQKLAAVSDLNPNAPRNSRNFVFQTVSPGKKEETTDKPRSKVRKNIAVAMPSPKRFKRDSTPTVKSRSIFQLLE

Target ORF information:

RefSeq Version	XM_018248110.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis claspin S homeolog (clspn.S), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018248110.1

ORF Insert Sequence:

ATGGCTGATC AGAATAAATT ATTGGGTTGT GTGGAGGATA AAGATACAGA TGATGAAATC 
TTGGTTCGTA AAAAATCTAA AAAGAAGAAA GTATTGGTGG ATAGTGACAG TGACGAAGAA 
TTGGAAATGC GTAATTTTGC AGATAATGTA AAGGGGCACT CTGATAATGA GGAGAATGAG 
GAGACTATGT CTGCTTATAG AGAAAAACCA AGAAAGATCC GTTCAGCTGT ATTGGACAGT 
GACAATAGTG ATCATGAGCT TGATGTTCAA ATAAGTACAA GTCAAAATGC AGCTGAAATA 
CCTGAGTCAG AACATGGTAG CTTGGAGAAG GAAACTCATA CTGTGAAGCC TAAAACAAGC 
AAGTCCTTGA AAAAACAAAC TGACACTAAT AAAGAGGAAA TCGTGAAGAA TAAATCAAAG 
CGCAAAATTC CGAAAGAGGA GAAGATTAAA AGGAGGACAA AACAGAAGTC AAAAGCAGTT 
GCTGAAGCTA GGCCAAATTT AAATGACAGT GGCTGCCTAC TCACAGATGG AGATCTTTTT 
GACAATGGGG TGGAAAATGA GATGGATTCT AATGAAGAAG AGGATTCTCT TGAAGCTATC 
CGGGCAAAAA TGAAAAGCAA ACTGAATAGT CATTCTGCTG AAAATTTTGA AGACTTTGAA 
CTTGATACTG AAGGCAATCA AGAATCCCCA GAAAAAAGAA AGGAACGAAA AGCTGCGCGA 
CTTGGTAAAG AAGCCATGAA ACAAATGCAC AGTGAGACCC AAAGACTAAT ACGTGAATCT 
TCTGTATCTT TACCATATCA TCTACCTGAA CCAAAAACAA TCCATGATTT TTTCAAAAGG 
CGTCCAAGGC CTCTTTGTCA AGGAAATGCA ATGCAGCTTA TAAAGTCAAC AAAATACCAG 
CCCTGCACTG AAGAGAAAAA AAAACCCAAT GAGGAAATAT GTGCTGAAGT TCCAGAGTTT 
GATTATGTTT CAAAGGAAGA TTTAGAAATC AGTCCAGAGC AACCTTTACT AAATACTCAG 
TGTTCACATG CTGCAGTCCT ATGTGTTGTG CAAAATGATG CTCGGACTGA GGGGTTAAGT 
AAATCCACAG AGGCAGTTGT GACTGGTCAA ATGAATGACC ATGAGGATGC TTTCAGTGAT 
TCAAACATTG TTCATGAACA AGAAACAGTT GGATTAATAA CCGTAACTGA AACCTTTCAG 
ACACCCTTTA TTCCCCAACC AGAGAGCGTA GTATGTGAAC AAATCCAGAA TGATGTAGTA 
GAGATGCAAC GTATGCCTGA ACAACCCACG CATAAACCCA AGTTATCCAA GCTTGAAAAG 
CTGAAAGCTC TTGGAGTGGA CTTGTCTATA AAACCTCGCC TTTGCCCTGA TGATGGTTCT 
TTTGTCAACT TGGATGAACC AAAGCCGAAT AAAGAATTTG AAGCTTTGAA GGAGCGTTTC 
CTGAAGCACA CTCTGCAAAA GTCCAAACCC AGAACTGAGC GGAAAGTCAA TCTTAATATT 
ATCCGCAAGG AGACCACTGC TGATGGAAAA GAAGAACTAA AAGCAGACGT TGTGCCAATA 
GTTATGGCTA CAGAAAAACC AGACAAGAGC ATTTATCAAA AGCCAGGTGA GAAGCTGCAG 
GTATTGAAAG TCAAACTGCA GGAAGCAATG AAAATCCGTC GCAGTGAGGA GCGCCTGAAG 
CGGCAAGCCT TGTATAAGCT TGACAATGAA GATGGCTTTG AAGATGATGA AGAGGAGGAA 
GAAATGACAG AGGAGTCTGA AGATGATGGG GATGGAAATG CTGAGACTGC AGATTATCCT 
GGAGGGGAAG ATGAGGAAGA GGTTGGTGAT GCTGAAGATG ACAATGATGA GGATGATACT 
GTAAATGATA GATTGTTGGG AAATGTGCCT GAAATTGTTA TCCCACTGCC GAGACCAGTA 
ACTACTGATT CTAGCCTCAT GCTGTTCAAG GACAATTCTT CAAAGCTAGG AGATTCGCTA 
CCTGATGAAA GTGGATGCAA GAGAAGCAGC AGGCTAGAAT ATGAAGAAGA CTCCCTGTTG 
CCACAATTAA AAGAAAACAG CCATAATAGT AGCTTTGAAC TTATTAGTTC AATGATACCA 
TCATACCAGC CATGTAATAA AACAACTCGA GTTGTGATCA ACTCCAATAA CCTTGGCTTT 
CGCTCACCAT CTCCGGTTCA TTTCAAAACA AGTTTTCTCA GCTCTGCATC AAAGAGTTCT 
GGCAAGATGT CTGAACCATC CCTTCCCGTG GAAGACTCAC AGGATCTATA TAATGCTTCC 
CCAGAGCCCA AAGCCTCATA TCTCTGTGCT GGAAGAAACT CTCAATTTCA ATTTTCGTTG 
GAGGATGACA CCCAGAGCCA ACTGCTTGAT GCTGATGGGT TTCTGAATGT TGGTCGCCAT 
AAATCTAGCT CTGCCAAACA CAGGCTAGCT TTGGATACAA TGGACGAGAA TGCTATGGAT 
GCCAATATGG ATGAACTACT AGACCTTTGC TCAGGACAGT TCAAAGAATC TCTTTCAGGC 
ACATCACAGG CAGCTGAAAG TGATGCTAAG AAACAACCAA TGGATGAATT GCTTGAATTG 
TGTTCTGGCA AATTTGTATC TCAAGCTGAC TGCTCCACAC AAGATTCTTC TGCTTCAGCT 
AAGGACCGTT CTACAGCTGT AAAAAAGGAC ATTTCTGATG AAGTGGCAAC GGTTTCAAGT 
TCATTCCTTA CTGAGAGAGA ACAGGAAGAA GATGAGGAAG AAGAATTTGG TGAATTCAAG 
CTCTTACCCT GTGATGATTC GGAGAGCGAA AACGAAGAAC AAAATGAAGA GGAAGAAGAA 
GAAGAGGATG CTAAGGATGA TGAAGATGAG GAAGAAATTT TGCAGAAGCA GCAAAAGAGA 
AAATTGAGGC TGAATGACTT CATGGAAGAT GAAGCCGAAT TGTCTGGAAG TGATGTAGGT 
AGCGGAGATG AGTATGAAGG AGATGATGAT GAGTATGAGG AAGAAGCCAT AGATGAAGAT 
CTCCCATCTG ATGAGGAACT GCAGGATCAA GTCAATAAAA TTCATATGAA AGTTACCATG 
GATGAAGACC AGCGACAGCT TCGTTTCTAT CAGGAGCGGT ACCTGGCTGA TGGGGATCTC 
CATAGTGACG GACCAGGGAG AACAAGAAAG TTCAGATGGA AACATCTTGA TGATGCCTCA 
CAGGTGGACA TGTTCCGCCG AGACTCTGAA TTGGAAGAGG TGGACGGAGA GAATGAGGAA 
ACTGAGGAAA CCGAACTTAA ATGGAGGAAA GAGCGGTTCG AAAGGGAACA ATGGCTGCGA 
GAACAGCCAC AAGGTAGTAG AGATAACAAT GAGGAGGAGG AGGAGGATAT TGGAGAAGAC 
AGCCAGTTTA TGAAATTGGC AAAGAAGGTC ACTGCAAAAG CCCTACAGAG AAAAGTGAGT 
ACAGAGACTA ATGAACCAAA GAAACCTGGG CCTAGAAATC CATATGAAGT GATCAGGCCT 
TTCAGCCTCC CCAAGTTGCG TACTGGTTCG CTGTTAAGCA AACCAAAAGA AGTTTTACAG 
AAGCTGGCAG CTGTGTCAGA CCTGAATCCA AATGCACCTC GAAACTCAAG AAACTTTGTC 
TTCCAAACTG TCTCACCCGG AAAGAAAGAA GAAACTACAG ACAAGCCAAG ATCAAAGGTA 
CGAAAGAATA TAGCTGTTGC CATGCCTTCC CCTAAACGTT TTAAAAGGGA CAGCACCCCT 
ACTGTTAAAA GCCGCAGTAT ATTTCAGTTG TTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Claspin - checkpoint adaptor and DNA replication factor.
The FEBS journal286(3)441-455(2019 02)
Smits VAJ,Cabrera E,Freire R,Gillespie DA

SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin.
Cell cycle (Georgetown, Tex.)14(17)2777-84(2015)
Ryu H,Yoshida MM,Sridharan V,Kumagai A,Dunphy WG,Dasso M,Azuma Y

Phosphorylation of Claspin is triggered by the nucleocytoplasmic ratio at the Xenopus laevis midblastula transition.
Developmental biology353(2)302-8(2011 May)
Gotoh T,Kishimoto T,Sible JC

Drf1-dependent kinase interacts with Claspin through a conserved protein motif.
The Journal of biological chemistry285(17)12638-46(2010 Apr)
Gold DA,Dunphy WG

Claspin: timing the cell cycle arrest when the genome is damaged.
Cell cycle (Georgetown, Tex.)5(24)2831-4(2006 Dec)
Freire R,van Vugt MA,Mamely I,Medema RH

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clspn.S cDNA ORF clone, Xenopus laevis(African clawed frog)

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Target ORF information:

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