lama1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	lama1
Entrez Gene ID	101079183
Full Name	laminin subunit alpha-1-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029842715.1	XP_029698575.1	laminin subunit alpha-1 isoform X4
XM_011619215.1	XP_011617517.1	laminin subunit alpha-1-like
XM_029842712.1	XP_029698572.1	laminin subunit alpha-1 isoform X1
XM_029842713.1	XP_029698573.1	laminin subunit alpha-1 isoform X2
XM_029842714.1	XP_029698574.1	laminin subunit alpha-1 isoform X3

Pathways from BioSystems
Gene Ontology

The following lama1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lama1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo31636	XM_011619215.1 Latest version!	Takifugu rubripes laminin subunit alpha-1-like (LOC101079183), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo43637	XM_029842712.1 Latest version!	Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo43638	XM_029842713.1 Latest version!	Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo43639	XM_029842714.1 Latest version!	Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo43640	XM_029842715.1 Latest version!	Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lama1 ( XM_011619215.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011617517.1 Takifugu rubripes laminin subunit alpha-1-like (LOC101079183), mRNA.

Price & Availability↑

CloneID	OTo31636
Clone ID Related Accession (Same CDS sequence)	XM_011619215.1
Accession Version	XM_011619215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	laminin subunit alpha-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004072408.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 22% of CDS bases ##RefSeq-Attributes-END##

ATGGAGGCGG GGTCTCCCCC GGTTCCTCGG AAACGACATC CCATCACCAA CGCCATCGAC 
GGAACCAACC GCTGGTGGCA GAGTCCCAGT ATCAAGAATG GGCGGCAGTT TCACTGGGTC 
ACCATCACGC TGGACCTTAA ACAGATCTTC CAGGTCGCCT ACGTCATCAT CAAAGCAGCG 
AACTCTCCTC GCCCAGGTAA CTGGATCCTG GAGCGCTCCC TGGACGGGGT GACCTTTGAC 
CCCTGGCAGT ATTACGCAAT TAGCAACTCC GAGTGCTTGT CCCATTACAA CGTCACCCCG 
AGACTCGGAC CTCCGACCTA CAAGAGGGAC ACGGAGGTCA TCTGCACGTC CTACTACTCC 
AGACTGAACC CTCTGGAGAA CGGAGAGATC CACACCTCGC TGATCAACGG CCGCCCCGGA 
GCGGACGACC TGACGCCCGA GCTCTTGGAC TTCACCTCGG CCCGTTACAT CCGACTCAGG 
CTCCAGCGGA TCAGGACGCT CAACGCCGAC CTCATGATGC TGAGCACGCG GGACCCCCGA 
GACATCGACC CCATCGTCAC ACGGAGGTAT TATTACTCCA TCAAAGATGT GTCCGTGGGT 
GGAATGTGCA TCTGTTATGG CCACGCCCAG AATTGCCCCT TGGACCCGGT CACAAAGCTG 
CAGTCTCTCC GGTATTCAGA CTTTCCGTGT GTTGCCTGTA ACTGTGACCT GAGAGGATCA 
GAGTCACCTG TGTGCTCCAG GGACGACAAG CATCCGGGTC AGTGTGTGTG TAAGGAGGGT 
TTCGCAGGTG AACGGTGCGA CACCTGTGCG TTTGGATACA GAGACTTTCC CCAGTGTGTT 
CGCTGTGAGT GTGACGCTGC TGGCAGCACC AACGCGGACC CCTGCAGACC CTGCACCTGC 
AAGGTGAACG TGATGGGAGC CCACTGTGAT CTGTGTAAAC CCGGATTCTA CAACCTCCAG 
GAGAAGAATC CGCTCGGCTG CACCGACTGT TTCTGCTTCG GCGTGTCCGA CGTCTGTGAG 
AGTTCTGCTT GGTCCACAAC TCAGGTGCTT CATAGAGATG CTTGGCTCCT CCCCCGCCCG 
GCTCCTCAGA ACATTACAGT CCTCCAGGAC AATGATTTAC CCTCACCTGG AAACACTTCC 
TCTGGCCACT CCCACCAGGA CGTTCTGCTG TGGGATGCAC CTGACGGTTT CCTTGGTAAC 
AAGCTGGGCT CCTATGGAGG CTTCCTGAAC TACTCTCTGC TGTATGACAT TCCATCGGAC 
AATGAGGACC GCTCTATTCC CGCCCACTCT GACGTCATGA TCAAGGGAAA CGGTCGTGCT 
CTACATTCCA TCCCTCACTT CCTCTTTGTC TCTCCTCTTG CTGAGAGTGC GGTTGCCATA 
GTGATGGTTG ACGGACAGTT CAGGGATGTT CAGTCAGGCG ACCCCGTGAG CCGCGATGAG 
CTCCTGTCGG CCCTCGCCGA CGTTACGACT CTGACCGTCA GGGTCGGTCT GAACGCCACC 
GCAGATGGAC CGATACGTCT CAGCTGGATC TCCCTGGACG CTGCTGACCC GGGCCCAGGT 
TCTGCCCTGG ACGCCGCTGA CCCGGGCCCA GGTTCTGCCC AACAGGCGGT CGCCGTGGAA 
AAGTGTGAAT GTCCGTGGGG TTACACCGGC ACTTCCTGTG AGACGTGTCT CCCGGGGTTC 
TACAGAGTGG GCGGGGTTTT GTTCGGAGGA AACTGTATGC AGTGCGAGTG TAACGACCAC 
GCGTCAGAGT GTGACATCAG TGGCGACTGT TGGGGTTGTA CACATAACAC CAGCGGTCCT 
CGCTGTGAAC AGTGTCTCCC TGGTTTCTAT GGCAACGCCA CAGAGGGGAC AGCAGATGAC 
TGCCAGTTGT GTCCTTGTCC TCTGACAAAC CCGTCCAACA GTTTCAGTCC CACCTGCGTG 
CTGGAGGGGT CAGGAAAGGT GGTGTGTGAC CAGTGCCAGG AGGGACACAC AGGAAGCAAC 
TGTGAAAGGT GTGCGAGTGG TTTCTATGGT AACCCACGGG CAGCCAATGG CACCTGTGTG 
CGCTGTGATT GTCACGGTAA TGTGAACGCA AGTGAGGCGG GACACTGTGA CGTCATCACC 
GGCGAGTGCC TGCGGTGCCT TTTCAACACC GGCGGACGCC ACTGCGAGGT CTGTCAGCCC 
GGTTACTATG GAGACGCAGT GCTTGCCAAG AACTGTCAGG AATGTGGCTG TGACGTCAAC 
GGCGCCCCCT CCAGTGTGTG TGACGTCACA ACGGGTCAAT GTCTTTGCAA GGAAAACGTG 
ACAGGACGCA CTTGTGACCA GTGTCAGTCG GGCTTTTTCG GCCTCCACAG TGAGATGGGC 
TGTCAGCTCT GTGGCTGCAA CCAATCGGGA TCGCTGTCTG AGTCATGTGA CGAGCAGGGC 
CGCTGCCCGT GCGTGAAGGG TGTGGGGGGA GACAAGTGTG ACCGCTGTGG TCACGGCTAC 
TACGGCGTGG ACGTCAGCAG CTGCAGAGCA TGCAACTGTG TCGACACGGG AGGGAACTGT 
GATCCCCAGA GCGGGGAGTG CATGTGCCCC GCCTTTACAG AGGGACCCAA CTGCAACAGA 
TGTGAAGCTG GATACTGGGG TCACGACCCC ACAACGGGCT GCACGCCGTG CAGCTGCAGC 
AAGGACGGAA GCTCCGCCCC TGACTGTGAT GCGACCAGCG GCCAGTGTTG GTGCAGGAAG 
GGGTTTGGTG GCCGGTCATG TGACCACTGT GCCGCTGGGC ACCATGGTTA CCCGTCATGT 
TTACCATGCG GCTGTGACCT GGCAGGAACT GATGAAAAGT TCTGCAACAC GACGCTGAAC 
ACGTGTGACT GTCTGGACAC GGGAGTGTGT GTGTGCAAGG TGGGCGTGTC TGGGCGCCGC 
TGCGAGGAGT GTGTCGCCGG CCGATTCGCT CTGTCGGTGG ATAATCCCGA TGGCTGCTCT 
CCGTGTTTCT GTTCGGGGGT GAGCGGCGAC TGTGAGGAGC AGGGGGGGCT GATCCGACTA 
CCGGTCACTC TGGACCAGTC TCCTGGCCTC TTGTCAGTGG TTAGTCAGTT GAACCTGCAG 
GGTGTCGTGT CTGGAGTTTA CCAGCAGGGC GGCGACATGC TGCTCGACAC CAGGCACCTC 
AACACCAGTG GCTTGGCTGG CCCGCTCTAC TGGAGACTGC CCCCCCAGTT TGAAGGCCAA 
CAGCTTCTGT CCTACGGCGG CTCCTTATCC TACATCATCA CCTTTTATGC TGAGGATGGC 
GCTGGGCTGT CCAATCAGGA GCCCCAGATC ATGATAAAAG GTGGGACCCT CAAGAAGATG 
GTGATATACA TGGACGTGGT TGCCCCTAGC AACGGCATTA GGACAAACCA TGAGATCAGG 
ATGACGGAGC ACAAGTGGAA ATACTTTAAC TCAGTGTTGG AGACGTCGGT GGGTCGCTCG 
GACTTCATGT CCGTCCTGGG CAACATCGAG TACGTCCTCA TCAAAGCGTC TTACGGGAGC 
AGGCTGCAGC AGAGCAGGAT CTCAAATATT ACCATGGAAA CGGCGCTGGA GAACGAGGAC 
GGGTCGGAGG TCGGCGGGGA CATGGCAAGA TCGATCGAGT CCTGCATCTG TCCCACGGGA 
TACACCGGAC TGTCCTGTCA GGACTGTGCC GCTGGATTCT TCCGTCAGCC GCTGTCCGAG 
TTGTCACCTC AGAGTCAAAG GTTGATGTCG GTGCGTCCGT GCGTTCCGTG TCGCTGCAAC 
AACCACAGCG AGAGCTGTGA TGCAGCGAGT GGAGAATGTC AGCAGTGTCA GCATCACACC 
AGCGGGTGGA GCTGTCAGCA GTGTTCCCAA GGGTATCACG GGAACGTTAG CGGCTCCATA 
AGTGACTGCT CTCTTTGCGC CTGCCCCCTG AAGGACAACA GCTTCAGCCT CACCTGTGTG 
TCGGAGGGAG TGCTGGGAGA CTTCCGCTGC ACAGCCTGTG TTCCCGGATA CGAGGGGAGA 
TACTGTGAAA AGTGTTCCGT CGGTTACTAT GGTAACCCCA CTTTGCCAGG CGGCGTGTGT 
TCACGGTGTA ACTGTTCTGG GTGGGGTTCT CTTCAGCCGC TGTGTGACCA GCTGAATGGG 
AAGTGCGACT GTAAGGCTGG TGTCAAAGGT CAATCATGTG ACCAGTGCGA GGACAGACAC 
ATCCTGCAGG AAGGAGAGTG TGTGTCGTGT GAGGACAACT GCACCGGTGT GATGCTCAAC 
GACCTGGAAG ACTTGGACGA CCACCTCGCC GCCGTCAACA TCAGCAGCTT TGTTGTGGCC 
TCGTACCTTC GGTTAGCGGC GCTGGAGAAC CAGACCAGAG GCGTCCAGGT GGTAAATATG 
TCTGGTGACC TTCAGAGACT GGACACATCT GCTCAGGACA CCATCCATCA GGGCTCACAG 
CAAATCCAGA ACATCAACAT CCTTCAAGAC AAAGTGCAGG CTCTTCAGCA GGAGGCCGAA 
CGCCTGAATG CGACTGCAGA AGAAGAGCTG ACGACAGCCA ACAGAACACA TCTGCTGCAA 
GAACTTGAGC TGATGTTGAG AAGCATGAGA GTGACCAACC TCACCGCTGC CAGCAGCCTG 
GCCAATCAGG AGCTCCTACG CGCCACATCT GTCCTCTCCC TGCAGTCGGA CCTGCTCTCC 
CGCAGGGCCG CAGTGGACGA CGGCCTCCGC CACCTTTCGA CCTCCCTCAG CAGTAACAGC 
GGAACACTGC GCCACCTACA GGCACAACTG AGCGGCGCCA CGCAACAAAA CATGCAGACA 
CACGTGCTTC TGGAGGTCCT GCACAACCTG ACGCAGCAGC ACCAGTCTGT CCAGCAGGAC 
ATCAGCCAGG AGCGCCTCCT GGTGGCGGGA CTGATGCAGG ACACTCAGCT CGTGCTGGGC 
GACAGCCTCA GCATCATGGA GGACCTAACC AACACCACCT CGCAGGTGGA GGTGTTTGGC 
AGTTACCTGG ATCAGTGGAG CCCCCTGCTG AGGAAACGGG TGGATGGTTT GGTTGGTGGT 
CTGAAGATGA CGGATTCCCT GGAGAAGGTC TACCTTTCAG AGAAACACGC CCAGCAGCTG 
GAAAACGACG CCCACTCCCT GCACAGCTCG CTGCTGTCTG TGTATAATGC GTCTCAAAAT 
GGCAGCCAGC TGCTTCAGGG GGGGAACAGC GGGGTCCCGG AGCAGGTGGA GGCGCTGCGG 
CAGGAGGCCC TCATGGCCGA CCGGTCCGCC CGGCTGCTGT TCAACGCGAC GGTCCAATCA 
GAACCGCCCC TGCCAGAGGA GAGCAGGACT CGCCTGAACA TCAGCGCCGC TGTTTTGGAA 
ACCTGTCGAC AGATCGGCGC TTCATCCGCA GACTTGCAGA TGAACGTCTC CATGGTAACC 
AGCAGACTAG GATTATTTAA AGAAAGCTTC CAGAAGTTCG GTCTCCTATT TCATCATTCG 
GTCACAGAGC TGCAGAGCCT CTCAAATGGT TCACTGACCG AGCTGCAACA AGTTCAGCTG 
CAGGCTGCAG AAGCAGACTC CAGGCTACAG GGGGCGCTAC TGAGGCTACA GAAACTCAGA 
CAGCAGCTCC AGGATTCTTC CTCTGCAGTG GAAAACACCA GCAACACCGT CACAGAGACC 
CAGCAGCTCA CAGCCCTCAC ACACTCCACA GTCGATCAGG CGCGTCAGAA ACTGGGCGAG 
GTGGAGCGTC GCACAGAGCG CCTTATCGAC CAGATCAAGC CCCTGAGCAT GCTGGGAGAA 
ACGATAAACA GGAACCTGTC TGACATCAGA GAGCTGATCA ATCAGGCCCG AAGACAGGCC 
GCCTCGATTA AGGTGGCGGT GCAGGCAGAC AGAAGCTGCG TGCGCTCCTA CAGGCCACAG 
TCCGAGTCCA GGAACTTCAA CACGCTGAGT CTTGTCTTGA GGACGTCCAG TCCAAACAAC 
CTGCTGTTCT TCCAGGGCAG CAACACCACG GTGGACTTCC TGGCTCTGGA GATGTATCGT 
GGGAAGGTGT CTCTGCTGTG GGATCTGGGT TCAGGTAGCA CCAGACTGGA GTTTCCCGGC 
CTGGACATCA CCAACAACAG ATGGACCAGA ATCAACGCCA CACGGTTTGG GGCTCATGCG 
TCTCTGTGGG TTCATCAGCT GGAGTCGGAC TCGGAGCCCC TCCCTGCAGT AACGGCCACC 
TCCCCGGGTC CCGCCAGGAT TTTAGACACG GACAGAAACT CTTGGCTCTA CATTGGTGGA 
CTGGGAGCTC ACGGACAGAG GCCGGCAGCC CTGCGCTCCA CCACATTCCA GGGATGTCTG 
GGCGAAGCGG CCTTGAACGA GCGGAACATC GGCCTCTGGA ACTACCACAG AAGGGAGGGA 
GAGTGTGGAG GCTGCTTCAG CAGTCCACAA ACGGAGGAGA CCTCCTTCCA TTTCGACGGA 
TCCGGATTCT CTTTGGTCCA GAAGCCTCTA CGGGCGACCT CCACCTCCAT CGTCTTGCTG 
TTCAAAACAC TGTCACCAGG GGGGTTACTG TTGTACCTGG CCTCCAACAA CACAAGGGAC 
TTTCTGTCCA TCGAGCTAGT AGAGGGGCAG GTTCGCCTGA CCTTTGACCT CGGCTCAGGT 
GCACTCGTCC TGACCTCCAG CAGGAAGTAC AACACTGGCG TCTGGTACAA GATCACGCTG 
CAGAGGAACA AACGTAAAGG TTACCTTTCC ATCATGGCTG CTGACCAGCC GTCGGAAAAG 
GAGGTGTTGG AGGCGGAGTC TCCCGGGCTG GCGTCCGACC TTAACCGCTC CGACTTTGAC 
CCCATCTACA TCGGAGGACT TCCTGCCTCC AGACCCATCA GGCGACAGGT GGTGTCCAGG 
GCTTACGTGG GCTGTATAAA AAATGTGGAG ATCGCCCGGT CCAACTTTGA CCTGCTGAGA 
GACGCTTTTG GCGTCAGGAA GGGCTGCGTT CTTGAGGCCC TCAGGAGCGT GTCGCTGTTG 
ACCGGCGGCT TCGTTCAAAT GGCTCCTCGA GCTCTTGGAC CAGAGGTGGA GCTTCTCTTT 
TCCTTCTCCA CCAACAACCG GTCAGGAATT CTGCTCGCTG CCGTCACCGA CGACCACGTT 
CAGAGGCGGC GCTTCCTGTC CATCCACCTG GCCTCAGGTG CGGTGGAGGC CGACATTGGT 
GAAGTGGGAG GAGCCACACA AACCGTGACA GTGGTGAAAC CTAAGGGAGG ATTTTTTAGC 
GATGGTCTGA AACACTCTGT CATCGTTACC GTCAACAAGA AGACCCTGTC CATACAGGTG 
GATGAGACAC ACCTGAAGTC CATCCCTCTG TTGCCGGGCA GATTTTCTAA TTTGTCTCCA 
GCCTCATTTT TTATTGGTGG AGTACCTGTA GGGGCGGGGC TCCAGCTGCC CTCCAGGCTG 
CGGCAGATTT CTTTATCCTT CAGAGGCTGC ATCCAACACC TGGTGTTAGG AGGAGGAGCG 
CTCGTTGACC TCTCAGGAGC GTTGAGGTAT GAGGGGGTGA CCCTGGACAG CTGCCTGCTG 
GAAGAGGCGG CCAGGGGGGC GGTGCTTCCT GATGACCCGG ACACAGAACC AACCATGGAC 
CCGGTCCGCC TCCCTGTGGT ACCGCCCACC CCGCTGGGTG TCCTGCCCCC AGGCGCTCTA 
ACGTGTGCAG CACAGGTGGA CTCCTCCTTC CTGCCCTCCG CAGCTCAGTT CGGTTTGTCC 
AAACACAGTC ACATGACGTT CCTGATCAAC CCCGGCGCCC TCAGGAAAAG TGTGTCTCTG 
AAGATATCGG CTCGGACCCG ATCTCCGGAC GGCCTGCTCC TCCTCCTCTC CGACGCCAAA 
CAGATGGACT TCGCCGTCGT CAAACTGGCG GGGGGGAAGG TGACGTTGTC AGCCGACCTG 
GGGAAGGGTC CCACCTCCGT CACCTCACCT GTGTCCATCA CTGACGGACT CTGGCACACT 
GTGAGCGCCG AGGTCAGCCG CCGCTCGCTC GTCCTGGCCG TCGACGGTTC AAAGCCCGGC 
TCGGCGTCCA TCAGGGGGAA CCAGCTGGAC GTGGACAGGC GCCTGTACCT GGGGGGGCTG 
CCGCACGCAC ACGCCACCAG GAGGATTAAC GTAGGCGTCC CCGACTCGGT CAGCAGCAGC 
TTCCAGGGCT GCGTTCGGTC CGTCCACCTG AACGGCGCCG TGTTGGATCT GACCAAACCG 
GCCTCGCGCC ACAACGTCAC TTCCTGTTTC ACCGATGACC AGGCAGGAAG CTACTTCGAC 
GGCAGCGGAC ACGCCCTCTT CATGCGCGAT GGCTATAAAG TCGGATCCGA TGTTTCTGTA 
TCTCTGGAGT TCCGGACCAG CCAATCAGAA GGAGTGTTTT TAGGAATTAG CAGTGCCAAA 
ATTGATGCCA TCGGACTCGA GATGATCAAA GGACAGGTGG TGTTTAACGT GAACAATGGC 
GCCGGGAGAG TGACGGTGCG CTCCGTCGGT CCGATGCTGT GTGACGGCCG ATGGCACCGC 
GTCCTGGCCA GGAAAACCAA ACACAGCCTG AATTTACAGG TGGATGGGCG GAGCTACATC 
ACCCCCAACC CTTACCCACA ATCCACCTCG GCTGAGACGA AAAACCCAGT TTACCTGGGA 
GGCTACCCTG TCGGGGTAAA GCAGAACTGC CTGTCAATCA CCACCCGGTT CAGAGGTTGT 
CTGAAGAAGA TACAGCTCAT TAAACCGCAC CTGAGCGATA TGCTGGACCT AAGCTCCGCC 
CACGTCCTCT CAGGTGTCAC TCCTGACTCC TGCCCTGTAG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011617517.1
CDS	1..8685
Translation	MEAGSPPVPRKRHPITNAIDGTNRWWQSPSIKNGRQFHWVTITLDLKQIFQVAYVIIKAANSPRPGNWILERSLDGVTFDPWQYYAISNSECLSHYNVTPRLGPPTYKRDTEVICTSYYSRLNPLENGEIHTSLINGRPGADDLTPELLDFTSARYIRLRLQRIRTLNADLMMLSTRDPRDIDPIVTRRYYYSIKDVSVGGMCICYGHAQNCPLDPVTKLQSLRYSDFPCVACNCDLRGSESPVCSRDDKHPGQCVCKEGFAGERCDTCAFGYRDFPQCVRCECDAAGSTNADPCRPCTCKVNVMGAHCDLCKPGFYNLQEKNPLGCTDCFCFGVSDVCESSAWSTTQVLHRDAWLLPRPAPQNITVLQDNDLPSPGNTSSGHSHQDVLLWDAPDGFLGNKLGSYGGFLNYSLLYDIPSDNEDRSIPAHSDVMIKGNGRALHSIPHFLFVSPLAESAVAIVMVDGQFRDVQSGDPVSRDELLSALADVTTLTVRVGLNATADGPIRLSWISLDAADPGPGSALDAADPGPGSAQQAVAVEKCECPWGYTGTSCETCLPGFYRVGGVLFGGNCMQCECNDHASECDISGDCWGCTHNTSGPRCEQCLPGFYGNATEGTADDCQLCPCPLTNPSNSFSPTCVLEGSGKVVCDQCQEGHTGSNCERCASGFYGNPRAANGTCVRCDCHGNVNASEAGHCDVITGECLRCLFNTGGRHCEVCQPGYYGDAVLAKNCQECGCDVNGAPSSVCDVTTGQCLCKENVTGRTCDQCQSGFFGLHSEMGCQLCGCNQSGSLSESCDEQGRCPCVKGVGGDKCDRCGHGYYGVDVSSCRACNCVDTGGNCDPQSGECMCPAFTEGPNCNRCEAGYWGHDPTTGCTPCSCSKDGSSAPDCDATSGQCWCRKGFGGRSCDHCAAGHHGYPSCLPCGCDLAGTDEKFCNTTLNTCDCLDTGVCVCKVGVSGRRCEECVAGRFALSVDNPDGCSPCFCSGVSGDCEEQGGLIRLPVTLDQSPGLLSVVSQLNLQGVVSGVYQQGGDMLLDTRHLNTSGLAGPLYWRLPPQFEGQQLLSYGGSLSYIITFYAEDGAGLSNQEPQIMIKGGTLKKMVIYMDVVAPSNGIRTNHEIRMTEHKWKYFNSVLETSVGRSDFMSVLGNIEYVLIKASYGSRLQQSRISNITMETALENEDGSEVGGDMARSIESCICPTGYTGLSCQDCAAGFFRQPLSELSPQSQRLMSVRPCVPCRCNNHSESCDAASGECQQCQHHTSGWSCQQCSQGYHGNVSGSISDCSLCACPLKDNSFSLTCVSEGVLGDFRCTACVPGYEGRYCEKCSVGYYGNPTLPGGVCSRCNCSGWGSLQPLCDQLNGKCDCKAGVKGQSCDQCEDRHILQEGECVSCEDNCTGVMLNDLEDLDDHLAAVNISSFVVASYLRLAALENQTRGVQVVNMSGDLQRLDTSAQDTIHQGSQQIQNINILQDKVQALQQEAERLNATAEEELTTANRTHLLQELELMLRSMRVTNLTAASSLANQELLRATSVLSLQSDLLSRRAAVDDGLRHLSTSLSSNSGTLRHLQAQLSGATQQNMQTHVLLEVLHNLTQQHQSVQQDISQERLLVAGLMQDTQLVLGDSLSIMEDLTNTTSQVEVFGSYLDQWSPLLRKRVDGLVGGLKMTDSLEKVYLSEKHAQQLENDAHSLHSSLLSVYNASQNGSQLLQGGNSGVPEQVEALRQEALMADRSARLLFNATVQSEPPLPEESRTRLNISAAVLETCRQIGASSADLQMNVSMVTSRLGLFKESFQKFGLLFHHSVTELQSLSNGSLTELQQVQLQAAEADSRLQGALLRLQKLRQQLQDSSSAVENTSNTVTETQQLTALTHSTVDQARQKLGEVERRTERLIDQIKPLSMLGETINRNLSDIRELINQARRQAASIKVAVQADRSCVRSYRPQSESRNFNTLSLVLRTSSPNNLLFFQGSNTTVDFLALEMYRGKVSLLWDLGSGSTRLEFPGLDITNNRWTRINATRFGAHASLWVHQLESDSEPLPAVTATSPGPARILDTDRNSWLYIGGLGAHGQRPAALRSTTFQGCLGEAALNERNIGLWNYHRREGECGGCFSSPQTEETSFHFDGSGFSLVQKPLRATSTSIVLLFKTLSPGGLLLYLASNNTRDFLSIELVEGQVRLTFDLGSGALVLTSSRKYNTGVWYKITLQRNKRKGYLSIMAADQPSEKEVLEAESPGLASDLNRSDFDPIYIGGLPASRPIRRQVVSRAYVGCIKNVEIARSNFDLLRDAFGVRKGCVLEALRSVSLLTGGFVQMAPRALGPEVELLFSFSTNNRSGILLAAVTDDHVQRRRFLSIHLASGAVEADIGEVGGATQTVTVVKPKGGFFSDGLKHSVIVTVNKKTLSIQVDETHLKSIPLLPGRFSNLSPASFFIGGVPVGAGLQLPSRLRQISLSFRGCIQHLVLGGGALVDLSGALRYEGVTLDSCLLEEAARGAVLPDDPDTEPTMDPVRLPVVPPTPLGVLPPGALTCAAQVDSSFLPSAAQFGLSKHSHMTFLINPGALRKSVSLKISARTRSPDGLLLLLSDAKQMDFAVVKLAGGKVTLSADLGKGPTSVTSPVSITDGLWHTVSAEVSRRSLVLAVDGSKPGSASIRGNQLDVDRRLYLGGLPHAHATRRINVGVPDSVSSSFQGCVRSVHLNGAVLDLTKPASRHNVTSCFTDDQAGSYFDGSGHALFMRDGYKVGSDVSVSLEFRTSQSEGVFLGISSAKIDAIGLEMIKGQVVFNVNNGAGRVTVRSVGPMLCDGRWHRVLARKTKHSLNLQVDGRSYITPNPYPQSTSAETKNPVYLGGYPVGVKQNCLSITTRFRGCLKKIQLIKPHLSDMLDLSSAHVLSGVTPDSCPVG

Target ORF information:

RefSeq Version	XM_011619215.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes laminin subunit alpha-1-like (LOC101079183), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011619215.1

ORF Insert Sequence:

ATGGAGGCGG GGTCTCCCCC GGTTCCTCGG AAACGACATC CCATCACCAA CGCCATCGAC 
GGAACCAACC GCTGGTGGCA GAGTCCCAGT ATCAAGAATG GGCGGCAGTT TCACTGGGTC 
ACCATCACGC TGGACCTTAA ACAGATCTTC CAGGTCGCCT ACGTCATCAT CAAAGCAGCG 
AACTCTCCTC GCCCAGGTAA CTGGATCCTG GAGCGCTCCC TGGACGGGGT GACCTTTGAC 
CCCTGGCAGT ATTACGCAAT TAGCAACTCC GAGTGCTTGT CCCATTACAA CGTCACCCCG 
AGACTCGGAC CTCCGACCTA CAAGAGGGAC ACGGAGGTCA TCTGCACGTC CTACTACTCC 
AGACTGAACC CTCTGGAGAA CGGAGAGATC CACACCTCGC TGATCAACGG CCGCCCCGGA 
GCGGACGACC TGACGCCCGA GCTCTTGGAC TTCACCTCGG CCCGTTACAT CCGACTCAGG 
CTCCAGCGGA TCAGGACGCT CAACGCCGAC CTCATGATGC TGAGCACGCG GGACCCCCGA 
GACATCGACC CCATCGTCAC ACGGAGGTAT TATTACTCCA TCAAAGATGT GTCCGTGGGT 
GGAATGTGCA TCTGTTATGG CCACGCCCAG AATTGCCCCT TGGACCCGGT CACAAAGCTG 
CAGTCTCTCC GGTATTCAGA CTTTCCGTGT GTTGCCTGTA ACTGTGACCT GAGAGGATCA 
GAGTCACCTG TGTGCTCCAG GGACGACAAG CATCCGGGTC AGTGTGTGTG TAAGGAGGGT 
TTCGCAGGTG AACGGTGCGA CACCTGTGCG TTTGGATACA GAGACTTTCC CCAGTGTGTT 
CGCTGTGAGT GTGACGCTGC TGGCAGCACC AACGCGGACC CCTGCAGACC CTGCACCTGC 
AAGGTGAACG TGATGGGAGC CCACTGTGAT CTGTGTAAAC CCGGATTCTA CAACCTCCAG 
GAGAAGAATC CGCTCGGCTG CACCGACTGT TTCTGCTTCG GCGTGTCCGA CGTCTGTGAG 
AGTTCTGCTT GGTCCACAAC TCAGGTGCTT CATAGAGATG CTTGGCTCCT CCCCCGCCCG 
GCTCCTCAGA ACATTACAGT CCTCCAGGAC AATGATTTAC CCTCACCTGG AAACACTTCC 
TCTGGCCACT CCCACCAGGA CGTTCTGCTG TGGGATGCAC CTGACGGTTT CCTTGGTAAC 
AAGCTGGGCT CCTATGGAGG CTTCCTGAAC TACTCTCTGC TGTATGACAT TCCATCGGAC 
AATGAGGACC GCTCTATTCC CGCCCACTCT GACGTCATGA TCAAGGGAAA CGGTCGTGCT 
CTACATTCCA TCCCTCACTT CCTCTTTGTC TCTCCTCTTG CTGAGAGTGC GGTTGCCATA 
GTGATGGTTG ACGGACAGTT CAGGGATGTT CAGTCAGGCG ACCCCGTGAG CCGCGATGAG 
CTCCTGTCGG CCCTCGCCGA CGTTACGACT CTGACCGTCA GGGTCGGTCT GAACGCCACC 
GCAGATGGAC CGATACGTCT CAGCTGGATC TCCCTGGACG CTGCTGACCC GGGCCCAGGT 
TCTGCCCTGG ACGCCGCTGA CCCGGGCCCA GGTTCTGCCC AACAGGCGGT CGCCGTGGAA 
AAGTGTGAAT GTCCGTGGGG TTACACCGGC ACTTCCTGTG AGACGTGTCT CCCGGGGTTC 
TACAGAGTGG GCGGGGTTTT GTTCGGAGGA AACTGTATGC AGTGCGAGTG TAACGACCAC 
GCGTCAGAGT GTGACATCAG TGGCGACTGT TGGGGTTGTA CACATAACAC CAGCGGTCCT 
CGCTGTGAAC AGTGTCTCCC TGGTTTCTAT GGCAACGCCA CAGAGGGGAC AGCAGATGAC 
TGCCAGTTGT GTCCTTGTCC TCTGACAAAC CCGTCCAACA GTTTCAGTCC CACCTGCGTG 
CTGGAGGGGT CAGGAAAGGT GGTGTGTGAC CAGTGCCAGG AGGGACACAC AGGAAGCAAC 
TGTGAAAGGT GTGCGAGTGG TTTCTATGGT AACCCACGGG CAGCCAATGG CACCTGTGTG 
CGCTGTGATT GTCACGGTAA TGTGAACGCA AGTGAGGCGG GACACTGTGA CGTCATCACC 
GGCGAGTGCC TGCGGTGCCT TTTCAACACC GGCGGACGCC ACTGCGAGGT CTGTCAGCCC 
GGTTACTATG GAGACGCAGT GCTTGCCAAG AACTGTCAGG AATGTGGCTG TGACGTCAAC 
GGCGCCCCCT CCAGTGTGTG TGACGTCACA ACGGGTCAAT GTCTTTGCAA GGAAAACGTG 
ACAGGACGCA CTTGTGACCA GTGTCAGTCG GGCTTTTTCG GCCTCCACAG TGAGATGGGC 
TGTCAGCTCT GTGGCTGCAA CCAATCGGGA TCGCTGTCTG AGTCATGTGA CGAGCAGGGC 
CGCTGCCCGT GCGTGAAGGG TGTGGGGGGA GACAAGTGTG ACCGCTGTGG TCACGGCTAC 
TACGGCGTGG ACGTCAGCAG CTGCAGAGCA TGCAACTGTG TCGACACGGG AGGGAACTGT 
GATCCCCAGA GCGGGGAGTG CATGTGCCCC GCCTTTACAG AGGGACCCAA CTGCAACAGA 
TGTGAAGCTG GATACTGGGG TCACGACCCC ACAACGGGCT GCACGCCGTG CAGCTGCAGC 
AAGGACGGAA GCTCCGCCCC TGACTGTGAT GCGACCAGCG GCCAGTGTTG GTGCAGGAAG 
GGGTTTGGTG GCCGGTCATG TGACCACTGT GCCGCTGGGC ACCATGGTTA CCCGTCATGT 
TTACCATGCG GCTGTGACCT GGCAGGAACT GATGAAAAGT TCTGCAACAC GACGCTGAAC 
ACGTGTGACT GTCTGGACAC GGGAGTGTGT GTGTGCAAGG TGGGCGTGTC TGGGCGCCGC 
TGCGAGGAGT GTGTCGCCGG CCGATTCGCT CTGTCGGTGG ATAATCCCGA TGGCTGCTCT 
CCGTGTTTCT GTTCGGGGGT GAGCGGCGAC TGTGAGGAGC AGGGGGGGCT GATCCGACTA 
CCGGTCACTC TGGACCAGTC TCCTGGCCTC TTGTCAGTGG TTAGTCAGTT GAACCTGCAG 
GGTGTCGTGT CTGGAGTTTA CCAGCAGGGC GGCGACATGC TGCTCGACAC CAGGCACCTC 
AACACCAGTG GCTTGGCTGG CCCGCTCTAC TGGAGACTGC CCCCCCAGTT TGAAGGCCAA 
CAGCTTCTGT CCTACGGCGG CTCCTTATCC TACATCATCA CCTTTTATGC TGAGGATGGC 
GCTGGGCTGT CCAATCAGGA GCCCCAGATC ATGATAAAAG GTGGGACCCT CAAGAAGATG 
GTGATATACA TGGACGTGGT TGCCCCTAGC AACGGCATTA GGACAAACCA TGAGATCAGG 
ATGACGGAGC ACAAGTGGAA ATACTTTAAC TCAGTGTTGG AGACGTCGGT GGGTCGCTCG 
GACTTCATGT CCGTCCTGGG CAACATCGAG TACGTCCTCA TCAAAGCGTC TTACGGGAGC 
AGGCTGCAGC AGAGCAGGAT CTCAAATATT ACCATGGAAA CGGCGCTGGA GAACGAGGAC 
GGGTCGGAGG TCGGCGGGGA CATGGCAAGA TCGATCGAGT CCTGCATCTG TCCCACGGGA 
TACACCGGAC TGTCCTGTCA GGACTGTGCC GCTGGATTCT TCCGTCAGCC GCTGTCCGAG 
TTGTCACCTC AGAGTCAAAG GTTGATGTCG GTGCGTCCGT GCGTTCCGTG TCGCTGCAAC 
AACCACAGCG AGAGCTGTGA TGCAGCGAGT GGAGAATGTC AGCAGTGTCA GCATCACACC 
AGCGGGTGGA GCTGTCAGCA GTGTTCCCAA GGGTATCACG GGAACGTTAG CGGCTCCATA 
AGTGACTGCT CTCTTTGCGC CTGCCCCCTG AAGGACAACA GCTTCAGCCT CACCTGTGTG 
TCGGAGGGAG TGCTGGGAGA CTTCCGCTGC ACAGCCTGTG TTCCCGGATA CGAGGGGAGA 
TACTGTGAAA AGTGTTCCGT CGGTTACTAT GGTAACCCCA CTTTGCCAGG CGGCGTGTGT 
TCACGGTGTA ACTGTTCTGG GTGGGGTTCT CTTCAGCCGC TGTGTGACCA GCTGAATGGG 
AAGTGCGACT GTAAGGCTGG TGTCAAAGGT CAATCATGTG ACCAGTGCGA GGACAGACAC 
ATCCTGCAGG AAGGAGAGTG TGTGTCGTGT GAGGACAACT GCACCGGTGT GATGCTCAAC 
GACCTGGAAG ACTTGGACGA CCACCTCGCC GCCGTCAACA TCAGCAGCTT TGTTGTGGCC 
TCGTACCTTC GGTTAGCGGC GCTGGAGAAC CAGACCAGAG GCGTCCAGGT GGTAAATATG 
TCTGGTGACC TTCAGAGACT GGACACATCT GCTCAGGACA CCATCCATCA GGGCTCACAG 
CAAATCCAGA ACATCAACAT CCTTCAAGAC AAAGTGCAGG CTCTTCAGCA GGAGGCCGAA 
CGCCTGAATG CGACTGCAGA AGAAGAGCTG ACGACAGCCA ACAGAACACA TCTGCTGCAA 
GAACTTGAGC TGATGTTGAG AAGCATGAGA GTGACCAACC TCACCGCTGC CAGCAGCCTG 
GCCAATCAGG AGCTCCTACG CGCCACATCT GTCCTCTCCC TGCAGTCGGA CCTGCTCTCC 
CGCAGGGCCG CAGTGGACGA CGGCCTCCGC CACCTTTCGA CCTCCCTCAG CAGTAACAGC 
GGAACACTGC GCCACCTACA GGCACAACTG AGCGGCGCCA CGCAACAAAA CATGCAGACA 
CACGTGCTTC TGGAGGTCCT GCACAACCTG ACGCAGCAGC ACCAGTCTGT CCAGCAGGAC 
ATCAGCCAGG AGCGCCTCCT GGTGGCGGGA CTGATGCAGG ACACTCAGCT CGTGCTGGGC 
GACAGCCTCA GCATCATGGA GGACCTAACC AACACCACCT CGCAGGTGGA GGTGTTTGGC 
AGTTACCTGG ATCAGTGGAG CCCCCTGCTG AGGAAACGGG TGGATGGTTT GGTTGGTGGT 
CTGAAGATGA CGGATTCCCT GGAGAAGGTC TACCTTTCAG AGAAACACGC CCAGCAGCTG 
GAAAACGACG CCCACTCCCT GCACAGCTCG CTGCTGTCTG TGTATAATGC GTCTCAAAAT 
GGCAGCCAGC TGCTTCAGGG GGGGAACAGC GGGGTCCCGG AGCAGGTGGA GGCGCTGCGG 
CAGGAGGCCC TCATGGCCGA CCGGTCCGCC CGGCTGCTGT TCAACGCGAC GGTCCAATCA 
GAACCGCCCC TGCCAGAGGA GAGCAGGACT CGCCTGAACA TCAGCGCCGC TGTTTTGGAA 
ACCTGTCGAC AGATCGGCGC TTCATCCGCA GACTTGCAGA TGAACGTCTC CATGGTAACC 
AGCAGACTAG GATTATTTAA AGAAAGCTTC CAGAAGTTCG GTCTCCTATT TCATCATTCG 
GTCACAGAGC TGCAGAGCCT CTCAAATGGT TCACTGACCG AGCTGCAACA AGTTCAGCTG 
CAGGCTGCAG AAGCAGACTC CAGGCTACAG GGGGCGCTAC TGAGGCTACA GAAACTCAGA 
CAGCAGCTCC AGGATTCTTC CTCTGCAGTG GAAAACACCA GCAACACCGT CACAGAGACC 
CAGCAGCTCA CAGCCCTCAC ACACTCCACA GTCGATCAGG CGCGTCAGAA ACTGGGCGAG 
GTGGAGCGTC GCACAGAGCG CCTTATCGAC CAGATCAAGC CCCTGAGCAT GCTGGGAGAA 
ACGATAAACA GGAACCTGTC TGACATCAGA GAGCTGATCA ATCAGGCCCG AAGACAGGCC 
GCCTCGATTA AGGTGGCGGT GCAGGCAGAC AGAAGCTGCG TGCGCTCCTA CAGGCCACAG 
TCCGAGTCCA GGAACTTCAA CACGCTGAGT CTTGTCTTGA GGACGTCCAG TCCAAACAAC 
CTGCTGTTCT TCCAGGGCAG CAACACCACG GTGGACTTCC TGGCTCTGGA GATGTATCGT 
GGGAAGGTGT CTCTGCTGTG GGATCTGGGT TCAGGTAGCA CCAGACTGGA GTTTCCCGGC 
CTGGACATCA CCAACAACAG ATGGACCAGA ATCAACGCCA CACGGTTTGG GGCTCATGCG 
TCTCTGTGGG TTCATCAGCT GGAGTCGGAC TCGGAGCCCC TCCCTGCAGT AACGGCCACC 
TCCCCGGGTC CCGCCAGGAT TTTAGACACG GACAGAAACT CTTGGCTCTA CATTGGTGGA 
CTGGGAGCTC ACGGACAGAG GCCGGCAGCC CTGCGCTCCA CCACATTCCA GGGATGTCTG 
GGCGAAGCGG CCTTGAACGA GCGGAACATC GGCCTCTGGA ACTACCACAG AAGGGAGGGA 
GAGTGTGGAG GCTGCTTCAG CAGTCCACAA ACGGAGGAGA CCTCCTTCCA TTTCGACGGA 
TCCGGATTCT CTTTGGTCCA GAAGCCTCTA CGGGCGACCT CCACCTCCAT CGTCTTGCTG 
TTCAAAACAC TGTCACCAGG GGGGTTACTG TTGTACCTGG CCTCCAACAA CACAAGGGAC 
TTTCTGTCCA TCGAGCTAGT AGAGGGGCAG GTTCGCCTGA CCTTTGACCT CGGCTCAGGT 
GCACTCGTCC TGACCTCCAG CAGGAAGTAC AACACTGGCG TCTGGTACAA GATCACGCTG 
CAGAGGAACA AACGTAAAGG TTACCTTTCC ATCATGGCTG CTGACCAGCC GTCGGAAAAG 
GAGGTGTTGG AGGCGGAGTC TCCCGGGCTG GCGTCCGACC TTAACCGCTC CGACTTTGAC 
CCCATCTACA TCGGAGGACT TCCTGCCTCC AGACCCATCA GGCGACAGGT GGTGTCCAGG 
GCTTACGTGG GCTGTATAAA AAATGTGGAG ATCGCCCGGT CCAACTTTGA CCTGCTGAGA 
GACGCTTTTG GCGTCAGGAA GGGCTGCGTT CTTGAGGCCC TCAGGAGCGT GTCGCTGTTG 
ACCGGCGGCT TCGTTCAAAT GGCTCCTCGA GCTCTTGGAC CAGAGGTGGA GCTTCTCTTT 
TCCTTCTCCA CCAACAACCG GTCAGGAATT CTGCTCGCTG CCGTCACCGA CGACCACGTT 
CAGAGGCGGC GCTTCCTGTC CATCCACCTG GCCTCAGGTG CGGTGGAGGC CGACATTGGT 
GAAGTGGGAG GAGCCACACA AACCGTGACA GTGGTGAAAC CTAAGGGAGG ATTTTTTAGC 
GATGGTCTGA AACACTCTGT CATCGTTACC GTCAACAAGA AGACCCTGTC CATACAGGTG 
GATGAGACAC ACCTGAAGTC CATCCCTCTG TTGCCGGGCA GATTTTCTAA TTTGTCTCCA 
GCCTCATTTT TTATTGGTGG AGTACCTGTA GGGGCGGGGC TCCAGCTGCC CTCCAGGCTG 
CGGCAGATTT CTTTATCCTT CAGAGGCTGC ATCCAACACC TGGTGTTAGG AGGAGGAGCG 
CTCGTTGACC TCTCAGGAGC GTTGAGGTAT GAGGGGGTGA CCCTGGACAG CTGCCTGCTG 
GAAGAGGCGG CCAGGGGGGC GGTGCTTCCT GATGACCCGG ACACAGAACC AACCATGGAC 
CCGGTCCGCC TCCCTGTGGT ACCGCCCACC CCGCTGGGTG TCCTGCCCCC AGGCGCTCTA 
ACGTGTGCAG CACAGGTGGA CTCCTCCTTC CTGCCCTCCG CAGCTCAGTT CGGTTTGTCC 
AAACACAGTC ACATGACGTT CCTGATCAAC CCCGGCGCCC TCAGGAAAAG TGTGTCTCTG 
AAGATATCGG CTCGGACCCG ATCTCCGGAC GGCCTGCTCC TCCTCCTCTC CGACGCCAAA 
CAGATGGACT TCGCCGTCGT CAAACTGGCG GGGGGGAAGG TGACGTTGTC AGCCGACCTG 
GGGAAGGGTC CCACCTCCGT CACCTCACCT GTGTCCATCA CTGACGGACT CTGGCACACT 
GTGAGCGCCG AGGTCAGCCG CCGCTCGCTC GTCCTGGCCG TCGACGGTTC AAAGCCCGGC 
TCGGCGTCCA TCAGGGGGAA CCAGCTGGAC GTGGACAGGC GCCTGTACCT GGGGGGGCTG 
CCGCACGCAC ACGCCACCAG GAGGATTAAC GTAGGCGTCC CCGACTCGGT CAGCAGCAGC 
TTCCAGGGCT GCGTTCGGTC CGTCCACCTG AACGGCGCCG TGTTGGATCT GACCAAACCG 
GCCTCGCGCC ACAACGTCAC TTCCTGTTTC ACCGATGACC AGGCAGGAAG CTACTTCGAC 
GGCAGCGGAC ACGCCCTCTT CATGCGCGAT GGCTATAAAG TCGGATCCGA TGTTTCTGTA 
TCTCTGGAGT TCCGGACCAG CCAATCAGAA GGAGTGTTTT TAGGAATTAG CAGTGCCAAA 
ATTGATGCCA TCGGACTCGA GATGATCAAA GGACAGGTGG TGTTTAACGT GAACAATGGC 
GCCGGGAGAG TGACGGTGCG CTCCGTCGGT CCGATGCTGT GTGACGGCCG ATGGCACCGC 
GTCCTGGCCA GGAAAACCAA ACACAGCCTG AATTTACAGG TGGATGGGCG GAGCTACATC 
ACCCCCAACC CTTACCCACA ATCCACCTCG GCTGAGACGA AAAACCCAGT TTACCTGGGA 
GGCTACCCTG TCGGGGTAAA GCAGAACTGC CTGTCAATCA CCACCCGGTT CAGAGGTTGT 
CTGAAGAAGA TACAGCTCAT TAAACCGCAC CTGAGCGATA TGCTGGACCT AAGCTCCGCC 
CACGTCCTCT CAGGTGTCAC TCCTGACTCC TGCCCTGTAG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lama1 ( XM_029842712.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029698572.1 Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X1, mRNA.

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CloneID	OTo43637
Clone ID Related Accession (Same CDS sequence)	XM_029842712.1
Accession Version	XM_029842712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	laminin subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGC TGCTGTTGGT GCTGCTGCTG GATGGAGCTG CAGCTCAGCT GAGAGGCCTG 
TTTCCTGCCA TCTTGAACTT GGCCACCAAC GCCGTGGTCG ACAGCAACGC CACGTGCGGC 
GACCCGGACC CGGAGGTCTA CTGTAAACTG GTGGAGCACG TTCCGGGTCG ACCCGTAAAG 
AACCCTCACT GCGCACGCTG TGACGCCGCC TCGCTTCTGC TGAGAGAACG ACATCCCATC 
ACCAACGCCA TCGACGGAAC CAACCGCTGG TGGCAGAGTC CCAGTATCAA GAATGGGCGG 
CAGTTTCACT GGGTCACCAT CACGCTGGAC CTGAAACAGA TCTTCCAGGT CGCCTACGTC 
ATCATCAAAG CAGCGAACTC TCCTCGCCCA GGTAACTGGA TCCTGGAGCG CTCCCTGGAC 
GGGGTGACCT TTGACCCCTG GCAGTATTAC GCAATTAGCA ACTCCGAGTG CTTGTCCCAT 
TACAACGTCA CCCCGAGACT CGGACCTCCG ACCTACAAGA GGGACACGGA GGTCATCTGC 
ACGTCCTACT ACTCCAGACT GAACCCTCTG GAGAACGGAG AGATCCACAC CTCGCTGATC 
AACGGCCGCC CCGGAGCGGA CGACCTGACG CCCGAGCTCT TGGACTTCAC CTCGGCCCGT 
TACATCCGAC TCAGGCTCCA GCGGATCAGG ACGCTCAACG CCGACCTCAT GATGCTGAGC 
ACGCGGGACC CCCGAGACAT CGACCCCATC GTCACACGGA GGTATTATTA CTCCATCAAA 
GATGTGTCCG TGGGTGGAAT GTGCATCTGT TATGGCCACG CCCAGAATTG CCCCTTGGAC 
CCGGTCACAA AGAAACTACA CTGTGTGTGC GAACACAACA CCTGTGGAGA GAACTGTAAC 
GAGTGTTGCC CTGGTTACCA TCAGCAGCCA TGGCAACCAG GAACGGTTCG TGAGGGAAAC 
ACCTGTGAAA AATGTAACTG TCACAACAAA GCAACCGACT GTTTCTACAA TCAGACGGTG 
GCCGACCTCT CTCTGAGTTT GAATATCCAC GGCGTCCGTC GAGGGGGCGG AGTCTGTGTC 
TCCTGTCTAC ACAACACCGC CGGGCTCAAC TGTGAGAGCT GCACTGATGG CTACTACAGA 
CCTGCTGAGG TCTCTCCGTA TTCAGACTTT CCGTGTGTTG CCTGTAACTG TGACCTGAGA 
GGATCAGAGT CACCTGTGTG CTCCAGGGAC GACAAGCATC CGGGTCAGTG TGTGTGTAAG 
GAGGGTTTCG CAGGTGAACG GTGCGACACC TGTGCGTTTG GATACAGAGA CTTTCCCCAG 
TGTGTTCGCT GTGAGTGTGA CGCTGCTGGC AGCACCAACG CGGACCCCTG CAGACCCTGC 
ACCTGCAAGG TGAACGTGAT GGGAGCCCAC TGTGATCTGT GTAAACCCGG ATTCTACAAC 
CTCCAGGAGA AGAATCCGCT CGGCTGCACC GACTGTTTCT GCTTCGGCGT GTCCGACGTC 
TGTGAGAGTT CTGCTTGGTC CACAACTCAG GTGCTTCATA GAGATGCTTG GCTCCTCCCC 
CGCCCGGCTC CTCAGAACAT TACAGTCCTC CAGGACAATG ATTTACCCTC ACCTGGAAAC 
ACTTCCTCTG GCCACTCCCA CCAGGACGTT CTGCTGTGGG ATGCACCTGA CGGTTTCCTT 
GGTAACAAGC TGGGCTCCTA TGGAGGCTTC CTGAACTACT CTCTGCTGTA TGACATTCCA 
TCGGACAATG AGGACCGCTC TATTCCCGCC CACTCTGACG TCATGATCAA GGGAAACGGT 
CGTGCTCTAC ATTCCATCCC TCACTTCCTC TTTGTCTCTC CTCTTGCTGA GAGTGTGGTT 
GCCATAGTGA TGGTTGACGG GCAGTTCAGG GATGTTCAGT CAGGCGACCC CGTGAGCCGC 
GATGAGCTCC TGTCGGCCCT CGCCGACGTT ACGACTCTGA CCGTCAGGGT CGGTCTGAAC 
GCCACCGCAG ATGGACCGAT ACGTCTCAGC TGGATCTCCC TGGACGCCGC TGACCCGGGC 
CCAGGTTCTG CCCTGGACGC CGCTGACCCG GGCCCAGGTT CTGCCCAAGA GGCGGTCGCC 
GTGGAAAAGT GTGAATGTCC GTGGGGTTAC ACCGGCACTT CCTGTGAGAC GTGTCTCCCG 
GGGTTCTACA GAGTGGGCGG GGTTTTGTTC GGAGGAAACT GTATGCAGTG CGAGTGTAAC 
GACCACGCGT CAGAGTGTGA CATCAGTGGC GACTGTTGGG GTTGTACACA TAACACCAGC 
GGTCCTCACT GTGAAGAGTG TCTCCCTGGT TTCTATGGCA ACGCCACAGA GGGGACAGCA 
GATGACTGCC AGTTGTGTCC TTGTCCTCTG ACAAACCCGT CCAACAGTTT CAGTCCCACC 
TGCGTGCTGG AAGGGTCAGG AAAGGTGGTG TGTGACCAGT GCCAGGAGGG ACACACAGGA 
AGCAACTGTG AAAGGTGTGC GAGTGGTTTC TATGGTAACC CACGGGCAGC CAATGGCACC 
TGTGTGCGCT GTGATTGTCA CGGTAATGTG AACGCAAGTG AGGCGGGACA CTGTGACGTC 
ATCACCGGCG AGTGCCTGCG GTGCCTTTTC AACACCGGCG GACGCCACTG CGAGGTCTGT 
CAGCCCGGTT ACTATGGAGA CGCAGTGCTT GCCAAGAACT GTCAGGAATG TGGCTGTGAC 
GTCAACGGCG CCCCCTCCAG TGTGTGTGAC GTCACAACGG GTCAATGTCT TTGCAAGGAA 
AACGTGACAG GACGCACTTG TGACCAGTGT CAGTCGGGCT TTTTCGGCCT CCGCAGTGAG 
ATGGGCTGTC AGCTCTGTGG CTGCAACCAA TCGGGATCGC TGTCTGAGTC ATGTGACGAG 
CAGGGCCGCT GCCCGTGCGT GAAGGGTGTG GGGGGAGACA AGTGTGACCG CTGTGGTCAC 
GGCTACTACG GCGTGGACGT CAGCAGCTGC AGAGCATGCA ACTGTGTCGA CACGGGAGGG 
AACTGTGATC CCCAGAGCGG GGAGTGCATG TGCCCCGCCT TTACAGAGGG ACCCAACTGC 
AACAGATGTG AAACTGGATA CTGGGGTCAC GACCCCACAA CTGGCTGCAC GCCGTGCAGC 
TGCAGCAAGG ACGGAAGCTC CGCCCCTGAC TGTGATGCGA CCAGCGGCCA GTGTTGGTGC 
AGGAAGGGGT TTGGTGGCCG GTCATGTGAC CACTGTGCCG CTGGGCACCA TGGTTACCCG 
TCATGTTTAC CATGCGGCTG TGACCTGGCA GGAACTGATG AAAAGTTCTG CAACACGACG 
CTGAACACGT GTGACTGTCT GGACACGGGA GTGTGTGTGT GCAAGGTGGG CGTGTCTGGG 
CGCCGCTGCG AGGAGTGTGT CGCCGGCCGA TTCGCTCTGT CGGTGGACAA TCCCGATGGC 
TGCTCTCCGT GTTTCTGTTC GGGGGTGAGC GGCGACTGTG AGGAGCAGGG GGGGCTGATC 
CGACTACCGG TCACTCTGGA CCAGTCTCCT GGCCTCTTGT CAGTGGTTAG TCAGTTGAAC 
TTGCAGGGTG TCGTGTCTGG AGTTTACCAG CAGGGCGGCG ACATGCTGCT CGACACCAGG 
CACCTCAACA CCAGTGGCTG GGCTGGCCCA CTCTACTGGA GACTGCCCCC CCAGTTTGAA 
GGCCAACAGC TTCTGTCCTA CGGCGGCTCC TTATCCTACA TCATCACCTT TTATGCTGAG 
GATGGCGCTG GGCTGTCCAA TCAGGAGCCC CAGATCATGA TAAAAGGTGG GACCCTCAAG 
AAGATGGTGA TATACATGGA CGTGGTTGCC CCTAGCAACG GCATTAGGAC AAACCATGAG 
ATCAGGATGA CGGAGCACAA GTGGAAATAC TTTAACTCAG TGTTGGAGAC GTCGGTGGGT 
CGCTCGGACT TCATGTCCGT CCTGGGCAAC ATCGAGTACG TCCTCATCAA AGCGTCTTAC 
GGGAGCAGGC TGCAGCAGAG CAGGATCTCA AATATTACCA TGGAAACGGC GCTGGAGAAC 
GAGGACGGGT CAGAGGTCGG CGGGGACATG GCAAGATCGA TCGAGTCCTG CATCTGTCCC 
ACGGGATACA CCGGACTGTC CTGTCAGGAC TGTGCCGCTG GATTCTTCCG TCAGCCGCTG 
TCCGAGTTGT CACCTCAGAG TCAAAGGTTG ATGTCGGTGC GTCCGTGCGT TCCGTGTCGC 
TGCAACAACC ACAGCGAGAG CTGTGATGCA GCGAGTGGAG AATGTCAGCA GTGTCAGCAT 
CACACCAGCG GGTGGAGCTG TCAGCAGTGT TCCCAAGGGT ATCACGGGAA CGTTAGCGGC 
TCCATAAGTG ACTGCTCTCT TTGCGCCTGC CCCCTGAAGG ACAACAGCTT CAGCCCCACC 
TGTGTGTCGG AGGGAGTGCT GGGAGACTTC CGCTGCACAG CCTGTGTTCC CGGATACGAG 
GGGAGATACT GTGAAAAGTG TTCCGTCGGT TACTATGGTA ACCCCACTTT GCCAGGCGGC 
GTGTGTTCAC GGTGTAACTG TTCTGGGTGG GGTTCTCTTC AGCCGCTGTG TGACCAGCTG 
AATGGGAAGT GCGACTGTAA GGCTGGTGTC AAAGGTCAAT CATGTGACCA GTGCGAGGAC 
AGACACATCC TGCAGGAAGG AGAGTGTGTG TCGTGTGAGG ACAACTGCAC CGGTGTGATG 
CTCAACGACC TGGAAGACTT GGACGACCAC CTCGCCGCCG TCAACATCAG CAGCTTTGTT 
GTGGCCTCGT ACCTTCGGTT AGCGGCGCTG GAGAACCAGA CCAGAGGCGT CCAGGCGATG 
GCGGCGGAAA ACACTTCAGC CTCTCTGCAG CTGAGCGGCC TCGAAGAGCA ACTCGGCCTC 
ATGAGCCTCG ACCTCGGCGT CTTGCTGCAG CTGGTGGTAA ATATGTCTGG TGACCTTCAG 
AGACTGGACA CATCTGCTCA GGACACCATC CATCAGGGCT CACAGCAAAT CCAGAACATC 
AACATCCTTC AAGACAAAGT GCAGGCTCTT CAGCAGGAGG CCGAACGCCT GAATGCGACT 
GCAGAAGAAG AGCTGACGAC AGCCAACAGA ACACATCTGC TGCAAGAACT TGAGTTGATG 
TTGAGAAGCA TGAGAGTGAC CAACCTCACC GCTGCCAGCA GCCTGGCCAA TCAGGAGCTC 
CTACGCGCCA CATCTGTCCT CTCCCTGCAG TCGGACCTGC TCTCCCGCAG GGCCGCAGTG 
GACGACGGCC TCCGCCACCT TTCGACCTCC CTCAGCAGTA ACAGCGGAAC ACTGCGCCAC 
CTACAGGCAC AACTGAGCGG CGCCACACAA CAAAACATGC AGACACACGT GCTTCTGGAG 
GTCCTGCACA ACCTGACGCA GCAGCACCAG TCTGTCCAGC AGGACATCAG CCAGGAGCGC 
CTCCTGGTGG CGGGACTGAT GCAGGACACT CAGCTCGTGC TGGGCGACAG CTTCAGCATC 
ATGGAGGACC TAACCAACAC CACCTCGCAG GTGGAGGTGT TTGGCAGTTA CCTGGATCAG 
TGGAGCCCCC TGCTGAGGAA ACGGGTGGAT GGTTTGGTTG GTGGTCTGAA GATGACGGAT 
TCCCTGGAGA AGGTCTACCT TTCAGAGAAA CACGCCCAGC AGCTGGACAA CGACGCCCAC 
TCCCTGCACA GCTCGCTGCT GTCTGTGTAT AATGCGTCTC AAAATGGCAG CCAGCTGCTT 
CAGGGGGGGA ACAGCGGCGT CCCGGAGCAG GTGGAGGCGC TGCGGCAGGA GGCCCTCATG 
GCCGACCGGT CCGCCCGGCT GCTGTTCAAC GCGACGGTCC AATCAGAACC GCCCCTGCCA 
GAGGAGAGCA GGACTCGCCT GAACATCAGC GCCGCTGTTT TGGAAACCTG TCGACAGATC 
GGCGCTTCAT CCGCAGACTT GCAGATGAAC GTCTCCATGG TAACCAGCAG ACTAGGATTA 
TTTAAAGAAA GCTTCCAGAA GTTCGGTCTC CTATTTCATC ATTCGATCAC AGAGCTGCAG 
AGCCTCTCAA ATGGTTCACT GACCGAGCTG CAACAAGTTC AGCTGCAGGC TGCAGAAGCA 
GACTCCAGGC TACAGGGGGC GCTACTGAGG CTACAGAAAC TCAGACAGCA GCTCCAGGAT 
TCTTCCTCTG CAGTGGAAAA CACCAGCAAC ACCGTCACAG AGACCCAGCA GCTCACAGCC 
CTCACACACT CCACAGTCGA TCAGGCGCGT CAGAAACTGG GCGAGGTGGA GCGTCGCACA 
GAGCGCCTTA TCGACCAGAT CAAGCCCCTG AGCATGCTGG GAGAAACGAT AAACAGGAAC 
CTGTCTGACA TCAGAGAGCT GATCAATCAG GCCCGAAGAC AGGCCGCCTC GATTAAGGTG 
GCGGTGCAGG CAGACAGAAG CTGCGTGCGC TCCTACAGGC CACAGTCCGA GTCCAGGAAC 
TTCAACACGC TGAGTCTTGT CTTGAGGACG TCCAGTCCAA ACAACCTGCT GTTCTTCCAG 
GGCAGCAACA CCACGGTGGA CTTCCTGGCT CTGGAGATGT ATCGTGGGAA GGTGTCTCTG 
CTGTGGGATC TGGGTTCAGG TAGCACCAGA CTGGAGTTTC CCGGACTGGA CATCACCAAC 
AACAGATGGA CCAGAATCAA CGCCACGCGG TTTGGGGCTC ATGCGTCTCT GTGGGTTCAT 
CAGCTGGAGT CGGACTCGGA GCCCCTCCCT GCAGTAACGG CCACCTCCCC GGGTCCCGCC 
AGGATTTTAG ACACGGACAG AAACTCTTGG CTCTACATTG GTGGACTGGG AGCTCACGGA 
CAGAGGCCGG CAGCCCTGCG CTCCACCACA TTCCAGGGAT GTCTGGGCGA AGCGGCCTTG 
AACGAGCGGA ACATCGGCCT CTGGAACTAC CACAGAAGGG AGGGAGAGTG TGGAGGCTGC 
TTCAGCAGTC CACAAACGGA GGAGACCTCC TTCCATTTCG ACGGATCCGG ATTCTCTTTG 
GTCCAGAAGC CTCTACGGGC GACCTCCACC TCCATCGTCT TGCTGTTCAA AACACTGTCA 
CCAGGGGGGT TACTGTTGTA CCTGGCCTCC AACAACACGA GGGACTTTCT GTCCATCGAG 
CTAGTAGAGG GGCAGGTTCG CCTGACCTTT GACCTCGGCT CAGGTGCACT CGTCCTGACC 
TCCAGCAGGA AGTACAACAC TGGCGTCTGG TACAAGATCA CGCTGCAGAG GAACAAACGT 
AAAGGTTACC TTTCCATCAT GGCTGCTGAC CAGTCGTCGG AAAAGGAGGT GTTGGAGGCG 
GAGTCTCCCG GGCTGGCGTC CGACCTTAAC CGCTCCGACT TTGACCCCAT CTACATCGGA 
GGACTTCCTG CCTCCAGACC CATCAGGCGA CAGGTGGTGT CCAGGGCTTA CGTGGGCTGT 
ATAAAAAATG TGGAGATCGC CCGGTCCAAC TTTGACCTGC TGAGAGACGC TTTTGGCGTC 
AGGAAGGGCT GCGTTCTTGA GGCCCTCAGG AGCGTGTCGC TGTTGACCGG CGGCTTCGTT 
CAAATGGCTC CTCGAGCTCT TGGACCAGAG GTGGAGCTTC TCTTTTCCTT CTCCACCAAC 
AACCGGTCAG GAATTCTGCT CGCTGCCGTC ACCGACGACC ACGTTCAGAG GCGGGTAGGG 
CGGAAAGTCC GCTTCCTGTC CATCCACCTG GCCTCAGGTG CGGTGGAGGC CGACATTGGT 
GAAGTGGGAG GAGCCACACA AACCGTGACA GTGGTGAAAC CTAAGGGAGG ATTTTTTAGC 
GATGGTCTGA AACACTCTGT CATCGTTACC GTCAACAAGA AGACCCTGTC CATACAGGTG 
GATGAGACAC ACCTGAAGTC CATCCCTCTG TTGCCGGGCA GATTTTCTAA TTTGTCTCCA 
GCCTCATTTT TTATTGGTGG AGTACCTGTA GGGGCGGGGC TCCAGCTGCC CTCCAGGCTG 
CGGCAGATTT CTTTATCCTT CAGAGGCTGC ATCCAACACC TGGTGTTAGG AGGAGGAGCG 
CTCGTTGACC TCTCAGGAGC GTTGAGGTAT GAGGGGGTGA CCCTGGACAG CTGCCTGCTG 
GAAGAGGCGG CCAGGGGGGC GGTGCTTCCT GATGACCCGG ACACAGAACC AACCATGGAC 
CCGGTCCGCC TCCCTGTGGT ACCGCCCACC CCGCTGGGTG TCCTGCCCCC AGGCGCTCTA 
ACGTGTGCAG CACAGGTGGA CTCCTCCTTC CTGCCCTCCG CAGCTCAGTT CGGTTTGTCC 
AAACACAGTC ACATGACGTT CCTGATCAAC CCCGGCGCCC TCAGGAAAAG TGTGTCTCTG 
AAGATATCGG CTCGGACCCG ATCTCCGGAC GGCCTGCTCC TCCTCCTCTC CGACGCCAAA 
CAGATGGACT TCGCCGTCGT CAAACTAGCG GGGGGGAAGG TGACGTTGTC AGCCGACCTG 
GGGAAGGGTC CCACCTCCGT CACCTCACCT GTGTCCATCA CTGACGGACT CTGGCACACT 
GTGAGCGCCG AGGTCAGCCG CCGCTCGCTC GTCCTGGCGG TCGACGGTTC AAAGCCCGGC 
TCGGCGTCCA TCAGGGGGAA CCAGCTGGAC GTGGACAGGC GCCTGTACCT GGGGGGGCTG 
CCGCACGCAC ACGCCACCAG GAGGATTAAC GTCAGCAGCA GCTTCCAGGG CTGCGTTCGG 
TCCGTCCACC TGAACGGCGC CGTGTTGGAT CTGACCAAAC CGGCCTCGCG CCACAACGTC 
ACTTCCTGTT TCACCGATGA CCAGGCAGGA AGCTACTTCG ACGGCAGCGG ACACGCCCTC 
TTCATGCGCG ATGGCTATAA AGTCGGATCC GATGTTTCTG TATCTCTGGA GTTCCGGACC 
AGCCAATCAG AAGGAGTGTT TTTAGGAATT AGCAGTGCCA AAATTGATGC CATCGGACTC 
GAGATGATCA AAGGACAGGT GGTGTTTAAC GTGAACAATG GCGCCGGGAG AGTGACGGTG 
CGCTCCGTCG GTCCGATGCT GTGTGACGGC CGATGGCACC GCGTCCTGGC CAGGAAAACC 
AAACACAGCC TGAATTTACA GGTGGATGGG CGGAGCTACA TCACCCCCAA CCCTTACCCA 
CAATCCACCT CGGCTGAGAC GAAAAACCCA GTTTACCTGG GAGGCTACCC TGTCGGGGTA 
AAGCAGAACT GCCTGTCAAT CACCACCCGG TTCAGAGGTT GTCTGAAGAA GATACAGCTC 
ATTAAACCGC ACCTGAGCGA TACGCTGGAC CTAAGCTCCG CCCACGTCCT CTCAGGTGTC 
ACTCCTGACT CCTGCCCTGT AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029698572.1
CDS	90..9356
Translation	MKLLLLVLLLDGAAAQLRGLFPAILNLATNAVVDSNATCGDPDPEVYCKLVEHVPGRPVKNPHCARCDAASLLLRERHPITNAIDGTNRWWQSPSIKNGRQFHWVTITLDLKQIFQVAYVIIKAANSPRPGNWILERSLDGVTFDPWQYYAISNSECLSHYNVTPRLGPPTYKRDTEVICTSYYSRLNPLENGEIHTSLINGRPGADDLTPELLDFTSARYIRLRLQRIRTLNADLMMLSTRDPRDIDPIVTRRYYYSIKDVSVGGMCICYGHAQNCPLDPVTKKLHCVCEHNTCGENCNECCPGYHQQPWQPGTVREGNTCEKCNCHNKATDCFYNQTVADLSLSLNIHGVRRGGGVCVSCLHNTAGLNCESCTDGYYRPAEVSPYSDFPCVACNCDLRGSESPVCSRDDKHPGQCVCKEGFAGERCDTCAFGYRDFPQCVRCECDAAGSTNADPCRPCTCKVNVMGAHCDLCKPGFYNLQEKNPLGCTDCFCFGVSDVCESSAWSTTQVLHRDAWLLPRPAPQNITVLQDNDLPSPGNTSSGHSHQDVLLWDAPDGFLGNKLGSYGGFLNYSLLYDIPSDNEDRSIPAHSDVMIKGNGRALHSIPHFLFVSPLAESVVAIVMVDGQFRDVQSGDPVSRDELLSALADVTTLTVRVGLNATADGPIRLSWISLDAADPGPGSALDAADPGPGSAQEAVAVEKCECPWGYTGTSCETCLPGFYRVGGVLFGGNCMQCECNDHASECDISGDCWGCTHNTSGPHCEECLPGFYGNATEGTADDCQLCPCPLTNPSNSFSPTCVLEGSGKVVCDQCQEGHTGSNCERCASGFYGNPRAANGTCVRCDCHGNVNASEAGHCDVITGECLRCLFNTGGRHCEVCQPGYYGDAVLAKNCQECGCDVNGAPSSVCDVTTGQCLCKENVTGRTCDQCQSGFFGLRSEMGCQLCGCNQSGSLSESCDEQGRCPCVKGVGGDKCDRCGHGYYGVDVSSCRACNCVDTGGNCDPQSGECMCPAFTEGPNCNRCETGYWGHDPTTGCTPCSCSKDGSSAPDCDATSGQCWCRKGFGGRSCDHCAAGHHGYPSCLPCGCDLAGTDEKFCNTTLNTCDCLDTGVCVCKVGVSGRRCEECVAGRFALSVDNPDGCSPCFCSGVSGDCEEQGGLIRLPVTLDQSPGLLSVVSQLNLQGVVSGVYQQGGDMLLDTRHLNTSGWAGPLYWRLPPQFEGQQLLSYGGSLSYIITFYAEDGAGLSNQEPQIMIKGGTLKKMVIYMDVVAPSNGIRTNHEIRMTEHKWKYFNSVLETSVGRSDFMSVLGNIEYVLIKASYGSRLQQSRISNITMETALENEDGSEVGGDMARSIESCICPTGYTGLSCQDCAAGFFRQPLSELSPQSQRLMSVRPCVPCRCNNHSESCDAASGECQQCQHHTSGWSCQQCSQGYHGNVSGSISDCSLCACPLKDNSFSPTCVSEGVLGDFRCTACVPGYEGRYCEKCSVGYYGNPTLPGGVCSRCNCSGWGSLQPLCDQLNGKCDCKAGVKGQSCDQCEDRHILQEGECVSCEDNCTGVMLNDLEDLDDHLAAVNISSFVVASYLRLAALENQTRGVQAMAAENTSASLQLSGLEEQLGLMSLDLGVLLQLVVNMSGDLQRLDTSAQDTIHQGSQQIQNINILQDKVQALQQEAERLNATAEEELTTANRTHLLQELELMLRSMRVTNLTAASSLANQELLRATSVLSLQSDLLSRRAAVDDGLRHLSTSLSSNSGTLRHLQAQLSGATQQNMQTHVLLEVLHNLTQQHQSVQQDISQERLLVAGLMQDTQLVLGDSFSIMEDLTNTTSQVEVFGSYLDQWSPLLRKRVDGLVGGLKMTDSLEKVYLSEKHAQQLDNDAHSLHSSLLSVYNASQNGSQLLQGGNSGVPEQVEALRQEALMADRSARLLFNATVQSEPPLPEESRTRLNISAAVLETCRQIGASSADLQMNVSMVTSRLGLFKESFQKFGLLFHHSITELQSLSNGSLTELQQVQLQAAEADSRLQGALLRLQKLRQQLQDSSSAVENTSNTVTETQQLTALTHSTVDQARQKLGEVERRTERLIDQIKPLSMLGETINRNLSDIRELINQARRQAASIKVAVQADRSCVRSYRPQSESRNFNTLSLVLRTSSPNNLLFFQGSNTTVDFLALEMYRGKVSLLWDLGSGSTRLEFPGLDITNNRWTRINATRFGAHASLWVHQLESDSEPLPAVTATSPGPARILDTDRNSWLYIGGLGAHGQRPAALRSTTFQGCLGEAALNERNIGLWNYHRREGECGGCFSSPQTEETSFHFDGSGFSLVQKPLRATSTSIVLLFKTLSPGGLLLYLASNNTRDFLSIELVEGQVRLTFDLGSGALVLTSSRKYNTGVWYKITLQRNKRKGYLSIMAADQSSEKEVLEAESPGLASDLNRSDFDPIYIGGLPASRPIRRQVVSRAYVGCIKNVEIARSNFDLLRDAFGVRKGCVLEALRSVSLLTGGFVQMAPRALGPEVELLFSFSTNNRSGILLAAVTDDHVQRRVGRKVRFLSIHLASGAVEADIGEVGGATQTVTVVKPKGGFFSDGLKHSVIVTVNKKTLSIQVDETHLKSIPLLPGRFSNLSPASFFIGGVPVGAGLQLPSRLRQISLSFRGCIQHLVLGGGALVDLSGALRYEGVTLDSCLLEEAARGAVLPDDPDTEPTMDPVRLPVVPPTPLGVLPPGALTCAAQVDSSFLPSAAQFGLSKHSHMTFLINPGALRKSVSLKISARTRSPDGLLLLLSDAKQMDFAVVKLAGGKVTLSADLGKGPTSVTSPVSITDGLWHTVSAEVSRRSLVLAVDGSKPGSASIRGNQLDVDRRLYLGGLPHAHATRRINVSSSFQGCVRSVHLNGAVLDLTKPASRHNVTSCFTDDQAGSYFDGSGHALFMRDGYKVGSDVSVSLEFRTSQSEGVFLGISSAKIDAIGLEMIKGQVVFNVNNGAGRVTVRSVGPMLCDGRWHRVLARKTKHSLNLQVDGRSYITPNPYPQSTSAETKNPVYLGGYPVGVKQNCLSITTRFRGCLKKIQLIKPHLSDTLDLSSAHVLSGVTPDSCPVG

Target ORF information:

RefSeq Version	XM_029842712.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029842712.1

ORF Insert Sequence:

ATGAAGTTGC TGCTGTTGGT GCTGCTGCTG GATGGAGCTG CAGCTCAGCT GAGAGGCCTG 
TTTCCTGCCA TCTTGAACTT GGCCACCAAC GCCGTGGTCG ACAGCAACGC CACGTGCGGC 
GACCCGGACC CGGAGGTCTA CTGTAAACTG GTGGAGCACG TTCCGGGTCG ACCCGTAAAG 
AACCCTCACT GCGCACGCTG TGACGCCGCC TCGCTTCTGC TGAGAGAACG ACATCCCATC 
ACCAACGCCA TCGACGGAAC CAACCGCTGG TGGCAGAGTC CCAGTATCAA GAATGGGCGG 
CAGTTTCACT GGGTCACCAT CACGCTGGAC CTGAAACAGA TCTTCCAGGT CGCCTACGTC 
ATCATCAAAG CAGCGAACTC TCCTCGCCCA GGTAACTGGA TCCTGGAGCG CTCCCTGGAC 
GGGGTGACCT TTGACCCCTG GCAGTATTAC GCAATTAGCA ACTCCGAGTG CTTGTCCCAT 
TACAACGTCA CCCCGAGACT CGGACCTCCG ACCTACAAGA GGGACACGGA GGTCATCTGC 
ACGTCCTACT ACTCCAGACT GAACCCTCTG GAGAACGGAG AGATCCACAC CTCGCTGATC 
AACGGCCGCC CCGGAGCGGA CGACCTGACG CCCGAGCTCT TGGACTTCAC CTCGGCCCGT 
TACATCCGAC TCAGGCTCCA GCGGATCAGG ACGCTCAACG CCGACCTCAT GATGCTGAGC 
ACGCGGGACC CCCGAGACAT CGACCCCATC GTCACACGGA GGTATTATTA CTCCATCAAA 
GATGTGTCCG TGGGTGGAAT GTGCATCTGT TATGGCCACG CCCAGAATTG CCCCTTGGAC 
CCGGTCACAA AGAAACTACA CTGTGTGTGC GAACACAACA CCTGTGGAGA GAACTGTAAC 
GAGTGTTGCC CTGGTTACCA TCAGCAGCCA TGGCAACCAG GAACGGTTCG TGAGGGAAAC 
ACCTGTGAAA AATGTAACTG TCACAACAAA GCAACCGACT GTTTCTACAA TCAGACGGTG 
GCCGACCTCT CTCTGAGTTT GAATATCCAC GGCGTCCGTC GAGGGGGCGG AGTCTGTGTC 
TCCTGTCTAC ACAACACCGC CGGGCTCAAC TGTGAGAGCT GCACTGATGG CTACTACAGA 
CCTGCTGAGG TCTCTCCGTA TTCAGACTTT CCGTGTGTTG CCTGTAACTG TGACCTGAGA 
GGATCAGAGT CACCTGTGTG CTCCAGGGAC GACAAGCATC CGGGTCAGTG TGTGTGTAAG 
GAGGGTTTCG CAGGTGAACG GTGCGACACC TGTGCGTTTG GATACAGAGA CTTTCCCCAG 
TGTGTTCGCT GTGAGTGTGA CGCTGCTGGC AGCACCAACG CGGACCCCTG CAGACCCTGC 
ACCTGCAAGG TGAACGTGAT GGGAGCCCAC TGTGATCTGT GTAAACCCGG ATTCTACAAC 
CTCCAGGAGA AGAATCCGCT CGGCTGCACC GACTGTTTCT GCTTCGGCGT GTCCGACGTC 
TGTGAGAGTT CTGCTTGGTC CACAACTCAG GTGCTTCATA GAGATGCTTG GCTCCTCCCC 
CGCCCGGCTC CTCAGAACAT TACAGTCCTC CAGGACAATG ATTTACCCTC ACCTGGAAAC 
ACTTCCTCTG GCCACTCCCA CCAGGACGTT CTGCTGTGGG ATGCACCTGA CGGTTTCCTT 
GGTAACAAGC TGGGCTCCTA TGGAGGCTTC CTGAACTACT CTCTGCTGTA TGACATTCCA 
TCGGACAATG AGGACCGCTC TATTCCCGCC CACTCTGACG TCATGATCAA GGGAAACGGT 
CGTGCTCTAC ATTCCATCCC TCACTTCCTC TTTGTCTCTC CTCTTGCTGA GAGTGTGGTT 
GCCATAGTGA TGGTTGACGG GCAGTTCAGG GATGTTCAGT CAGGCGACCC CGTGAGCCGC 
GATGAGCTCC TGTCGGCCCT CGCCGACGTT ACGACTCTGA CCGTCAGGGT CGGTCTGAAC 
GCCACCGCAG ATGGACCGAT ACGTCTCAGC TGGATCTCCC TGGACGCCGC TGACCCGGGC 
CCAGGTTCTG CCCTGGACGC CGCTGACCCG GGCCCAGGTT CTGCCCAAGA GGCGGTCGCC 
GTGGAAAAGT GTGAATGTCC GTGGGGTTAC ACCGGCACTT CCTGTGAGAC GTGTCTCCCG 
GGGTTCTACA GAGTGGGCGG GGTTTTGTTC GGAGGAAACT GTATGCAGTG CGAGTGTAAC 
GACCACGCGT CAGAGTGTGA CATCAGTGGC GACTGTTGGG GTTGTACACA TAACACCAGC 
GGTCCTCACT GTGAAGAGTG TCTCCCTGGT TTCTATGGCA ACGCCACAGA GGGGACAGCA 
GATGACTGCC AGTTGTGTCC TTGTCCTCTG ACAAACCCGT CCAACAGTTT CAGTCCCACC 
TGCGTGCTGG AAGGGTCAGG AAAGGTGGTG TGTGACCAGT GCCAGGAGGG ACACACAGGA 
AGCAACTGTG AAAGGTGTGC GAGTGGTTTC TATGGTAACC CACGGGCAGC CAATGGCACC 
TGTGTGCGCT GTGATTGTCA CGGTAATGTG AACGCAAGTG AGGCGGGACA CTGTGACGTC 
ATCACCGGCG AGTGCCTGCG GTGCCTTTTC AACACCGGCG GACGCCACTG CGAGGTCTGT 
CAGCCCGGTT ACTATGGAGA CGCAGTGCTT GCCAAGAACT GTCAGGAATG TGGCTGTGAC 
GTCAACGGCG CCCCCTCCAG TGTGTGTGAC GTCACAACGG GTCAATGTCT TTGCAAGGAA 
AACGTGACAG GACGCACTTG TGACCAGTGT CAGTCGGGCT TTTTCGGCCT CCGCAGTGAG 
ATGGGCTGTC AGCTCTGTGG CTGCAACCAA TCGGGATCGC TGTCTGAGTC ATGTGACGAG 
CAGGGCCGCT GCCCGTGCGT GAAGGGTGTG GGGGGAGACA AGTGTGACCG CTGTGGTCAC 
GGCTACTACG GCGTGGACGT CAGCAGCTGC AGAGCATGCA ACTGTGTCGA CACGGGAGGG 
AACTGTGATC CCCAGAGCGG GGAGTGCATG TGCCCCGCCT TTACAGAGGG ACCCAACTGC 
AACAGATGTG AAACTGGATA CTGGGGTCAC GACCCCACAA CTGGCTGCAC GCCGTGCAGC 
TGCAGCAAGG ACGGAAGCTC CGCCCCTGAC TGTGATGCGA CCAGCGGCCA GTGTTGGTGC 
AGGAAGGGGT TTGGTGGCCG GTCATGTGAC CACTGTGCCG CTGGGCACCA TGGTTACCCG 
TCATGTTTAC CATGCGGCTG TGACCTGGCA GGAACTGATG AAAAGTTCTG CAACACGACG 
CTGAACACGT GTGACTGTCT GGACACGGGA GTGTGTGTGT GCAAGGTGGG CGTGTCTGGG 
CGCCGCTGCG AGGAGTGTGT CGCCGGCCGA TTCGCTCTGT CGGTGGACAA TCCCGATGGC 
TGCTCTCCGT GTTTCTGTTC GGGGGTGAGC GGCGACTGTG AGGAGCAGGG GGGGCTGATC 
CGACTACCGG TCACTCTGGA CCAGTCTCCT GGCCTCTTGT CAGTGGTTAG TCAGTTGAAC 
TTGCAGGGTG TCGTGTCTGG AGTTTACCAG CAGGGCGGCG ACATGCTGCT CGACACCAGG 
CACCTCAACA CCAGTGGCTG GGCTGGCCCA CTCTACTGGA GACTGCCCCC CCAGTTTGAA 
GGCCAACAGC TTCTGTCCTA CGGCGGCTCC TTATCCTACA TCATCACCTT TTATGCTGAG 
GATGGCGCTG GGCTGTCCAA TCAGGAGCCC CAGATCATGA TAAAAGGTGG GACCCTCAAG 
AAGATGGTGA TATACATGGA CGTGGTTGCC CCTAGCAACG GCATTAGGAC AAACCATGAG 
ATCAGGATGA CGGAGCACAA GTGGAAATAC TTTAACTCAG TGTTGGAGAC GTCGGTGGGT 
CGCTCGGACT TCATGTCCGT CCTGGGCAAC ATCGAGTACG TCCTCATCAA AGCGTCTTAC 
GGGAGCAGGC TGCAGCAGAG CAGGATCTCA AATATTACCA TGGAAACGGC GCTGGAGAAC 
GAGGACGGGT CAGAGGTCGG CGGGGACATG GCAAGATCGA TCGAGTCCTG CATCTGTCCC 
ACGGGATACA CCGGACTGTC CTGTCAGGAC TGTGCCGCTG GATTCTTCCG TCAGCCGCTG 
TCCGAGTTGT CACCTCAGAG TCAAAGGTTG ATGTCGGTGC GTCCGTGCGT TCCGTGTCGC 
TGCAACAACC ACAGCGAGAG CTGTGATGCA GCGAGTGGAG AATGTCAGCA GTGTCAGCAT 
CACACCAGCG GGTGGAGCTG TCAGCAGTGT TCCCAAGGGT ATCACGGGAA CGTTAGCGGC 
TCCATAAGTG ACTGCTCTCT TTGCGCCTGC CCCCTGAAGG ACAACAGCTT CAGCCCCACC 
TGTGTGTCGG AGGGAGTGCT GGGAGACTTC CGCTGCACAG CCTGTGTTCC CGGATACGAG 
GGGAGATACT GTGAAAAGTG TTCCGTCGGT TACTATGGTA ACCCCACTTT GCCAGGCGGC 
GTGTGTTCAC GGTGTAACTG TTCTGGGTGG GGTTCTCTTC AGCCGCTGTG TGACCAGCTG 
AATGGGAAGT GCGACTGTAA GGCTGGTGTC AAAGGTCAAT CATGTGACCA GTGCGAGGAC 
AGACACATCC TGCAGGAAGG AGAGTGTGTG TCGTGTGAGG ACAACTGCAC CGGTGTGATG 
CTCAACGACC TGGAAGACTT GGACGACCAC CTCGCCGCCG TCAACATCAG CAGCTTTGTT 
GTGGCCTCGT ACCTTCGGTT AGCGGCGCTG GAGAACCAGA CCAGAGGCGT CCAGGCGATG 
GCGGCGGAAA ACACTTCAGC CTCTCTGCAG CTGAGCGGCC TCGAAGAGCA ACTCGGCCTC 
ATGAGCCTCG ACCTCGGCGT CTTGCTGCAG CTGGTGGTAA ATATGTCTGG TGACCTTCAG 
AGACTGGACA CATCTGCTCA GGACACCATC CATCAGGGCT CACAGCAAAT CCAGAACATC 
AACATCCTTC AAGACAAAGT GCAGGCTCTT CAGCAGGAGG CCGAACGCCT GAATGCGACT 
GCAGAAGAAG AGCTGACGAC AGCCAACAGA ACACATCTGC TGCAAGAACT TGAGTTGATG 
TTGAGAAGCA TGAGAGTGAC CAACCTCACC GCTGCCAGCA GCCTGGCCAA TCAGGAGCTC 
CTACGCGCCA CATCTGTCCT CTCCCTGCAG TCGGACCTGC TCTCCCGCAG GGCCGCAGTG 
GACGACGGCC TCCGCCACCT TTCGACCTCC CTCAGCAGTA ACAGCGGAAC ACTGCGCCAC 
CTACAGGCAC AACTGAGCGG CGCCACACAA CAAAACATGC AGACACACGT GCTTCTGGAG 
GTCCTGCACA ACCTGACGCA GCAGCACCAG TCTGTCCAGC AGGACATCAG CCAGGAGCGC 
CTCCTGGTGG CGGGACTGAT GCAGGACACT CAGCTCGTGC TGGGCGACAG CTTCAGCATC 
ATGGAGGACC TAACCAACAC CACCTCGCAG GTGGAGGTGT TTGGCAGTTA CCTGGATCAG 
TGGAGCCCCC TGCTGAGGAA ACGGGTGGAT GGTTTGGTTG GTGGTCTGAA GATGACGGAT 
TCCCTGGAGA AGGTCTACCT TTCAGAGAAA CACGCCCAGC AGCTGGACAA CGACGCCCAC 
TCCCTGCACA GCTCGCTGCT GTCTGTGTAT AATGCGTCTC AAAATGGCAG CCAGCTGCTT 
CAGGGGGGGA ACAGCGGCGT CCCGGAGCAG GTGGAGGCGC TGCGGCAGGA GGCCCTCATG 
GCCGACCGGT CCGCCCGGCT GCTGTTCAAC GCGACGGTCC AATCAGAACC GCCCCTGCCA 
GAGGAGAGCA GGACTCGCCT GAACATCAGC GCCGCTGTTT TGGAAACCTG TCGACAGATC 
GGCGCTTCAT CCGCAGACTT GCAGATGAAC GTCTCCATGG TAACCAGCAG ACTAGGATTA 
TTTAAAGAAA GCTTCCAGAA GTTCGGTCTC CTATTTCATC ATTCGATCAC AGAGCTGCAG 
AGCCTCTCAA ATGGTTCACT GACCGAGCTG CAACAAGTTC AGCTGCAGGC TGCAGAAGCA 
GACTCCAGGC TACAGGGGGC GCTACTGAGG CTACAGAAAC TCAGACAGCA GCTCCAGGAT 
TCTTCCTCTG CAGTGGAAAA CACCAGCAAC ACCGTCACAG AGACCCAGCA GCTCACAGCC 
CTCACACACT CCACAGTCGA TCAGGCGCGT CAGAAACTGG GCGAGGTGGA GCGTCGCACA 
GAGCGCCTTA TCGACCAGAT CAAGCCCCTG AGCATGCTGG GAGAAACGAT AAACAGGAAC 
CTGTCTGACA TCAGAGAGCT GATCAATCAG GCCCGAAGAC AGGCCGCCTC GATTAAGGTG 
GCGGTGCAGG CAGACAGAAG CTGCGTGCGC TCCTACAGGC CACAGTCCGA GTCCAGGAAC 
TTCAACACGC TGAGTCTTGT CTTGAGGACG TCCAGTCCAA ACAACCTGCT GTTCTTCCAG 
GGCAGCAACA CCACGGTGGA CTTCCTGGCT CTGGAGATGT ATCGTGGGAA GGTGTCTCTG 
CTGTGGGATC TGGGTTCAGG TAGCACCAGA CTGGAGTTTC CCGGACTGGA CATCACCAAC 
AACAGATGGA CCAGAATCAA CGCCACGCGG TTTGGGGCTC ATGCGTCTCT GTGGGTTCAT 
CAGCTGGAGT CGGACTCGGA GCCCCTCCCT GCAGTAACGG CCACCTCCCC GGGTCCCGCC 
AGGATTTTAG ACACGGACAG AAACTCTTGG CTCTACATTG GTGGACTGGG AGCTCACGGA 
CAGAGGCCGG CAGCCCTGCG CTCCACCACA TTCCAGGGAT GTCTGGGCGA AGCGGCCTTG 
AACGAGCGGA ACATCGGCCT CTGGAACTAC CACAGAAGGG AGGGAGAGTG TGGAGGCTGC 
TTCAGCAGTC CACAAACGGA GGAGACCTCC TTCCATTTCG ACGGATCCGG ATTCTCTTTG 
GTCCAGAAGC CTCTACGGGC GACCTCCACC TCCATCGTCT TGCTGTTCAA AACACTGTCA 
CCAGGGGGGT TACTGTTGTA CCTGGCCTCC AACAACACGA GGGACTTTCT GTCCATCGAG 
CTAGTAGAGG GGCAGGTTCG CCTGACCTTT GACCTCGGCT CAGGTGCACT CGTCCTGACC 
TCCAGCAGGA AGTACAACAC TGGCGTCTGG TACAAGATCA CGCTGCAGAG GAACAAACGT 
AAAGGTTACC TTTCCATCAT GGCTGCTGAC CAGTCGTCGG AAAAGGAGGT GTTGGAGGCG 
GAGTCTCCCG GGCTGGCGTC CGACCTTAAC CGCTCCGACT TTGACCCCAT CTACATCGGA 
GGACTTCCTG CCTCCAGACC CATCAGGCGA CAGGTGGTGT CCAGGGCTTA CGTGGGCTGT 
ATAAAAAATG TGGAGATCGC CCGGTCCAAC TTTGACCTGC TGAGAGACGC TTTTGGCGTC 
AGGAAGGGCT GCGTTCTTGA GGCCCTCAGG AGCGTGTCGC TGTTGACCGG CGGCTTCGTT 
CAAATGGCTC CTCGAGCTCT TGGACCAGAG GTGGAGCTTC TCTTTTCCTT CTCCACCAAC 
AACCGGTCAG GAATTCTGCT CGCTGCCGTC ACCGACGACC ACGTTCAGAG GCGGGTAGGG 
CGGAAAGTCC GCTTCCTGTC CATCCACCTG GCCTCAGGTG CGGTGGAGGC CGACATTGGT 
GAAGTGGGAG GAGCCACACA AACCGTGACA GTGGTGAAAC CTAAGGGAGG ATTTTTTAGC 
GATGGTCTGA AACACTCTGT CATCGTTACC GTCAACAAGA AGACCCTGTC CATACAGGTG 
GATGAGACAC ACCTGAAGTC CATCCCTCTG TTGCCGGGCA GATTTTCTAA TTTGTCTCCA 
GCCTCATTTT TTATTGGTGG AGTACCTGTA GGGGCGGGGC TCCAGCTGCC CTCCAGGCTG 
CGGCAGATTT CTTTATCCTT CAGAGGCTGC ATCCAACACC TGGTGTTAGG AGGAGGAGCG 
CTCGTTGACC TCTCAGGAGC GTTGAGGTAT GAGGGGGTGA CCCTGGACAG CTGCCTGCTG 
GAAGAGGCGG CCAGGGGGGC GGTGCTTCCT GATGACCCGG ACACAGAACC AACCATGGAC 
CCGGTCCGCC TCCCTGTGGT ACCGCCCACC CCGCTGGGTG TCCTGCCCCC AGGCGCTCTA 
ACGTGTGCAG CACAGGTGGA CTCCTCCTTC CTGCCCTCCG CAGCTCAGTT CGGTTTGTCC 
AAACACAGTC ACATGACGTT CCTGATCAAC CCCGGCGCCC TCAGGAAAAG TGTGTCTCTG 
AAGATATCGG CTCGGACCCG ATCTCCGGAC GGCCTGCTCC TCCTCCTCTC CGACGCCAAA 
CAGATGGACT TCGCCGTCGT CAAACTAGCG GGGGGGAAGG TGACGTTGTC AGCCGACCTG 
GGGAAGGGTC CCACCTCCGT CACCTCACCT GTGTCCATCA CTGACGGACT CTGGCACACT 
GTGAGCGCCG AGGTCAGCCG CCGCTCGCTC GTCCTGGCGG TCGACGGTTC AAAGCCCGGC 
TCGGCGTCCA TCAGGGGGAA CCAGCTGGAC GTGGACAGGC GCCTGTACCT GGGGGGGCTG 
CCGCACGCAC ACGCCACCAG GAGGATTAAC GTCAGCAGCA GCTTCCAGGG CTGCGTTCGG 
TCCGTCCACC TGAACGGCGC CGTGTTGGAT CTGACCAAAC CGGCCTCGCG CCACAACGTC 
ACTTCCTGTT TCACCGATGA CCAGGCAGGA AGCTACTTCG ACGGCAGCGG ACACGCCCTC 
TTCATGCGCG ATGGCTATAA AGTCGGATCC GATGTTTCTG TATCTCTGGA GTTCCGGACC 
AGCCAATCAG AAGGAGTGTT TTTAGGAATT AGCAGTGCCA AAATTGATGC CATCGGACTC 
GAGATGATCA AAGGACAGGT GGTGTTTAAC GTGAACAATG GCGCCGGGAG AGTGACGGTG 
CGCTCCGTCG GTCCGATGCT GTGTGACGGC CGATGGCACC GCGTCCTGGC CAGGAAAACC 
AAACACAGCC TGAATTTACA GGTGGATGGG CGGAGCTACA TCACCCCCAA CCCTTACCCA 
CAATCCACCT CGGCTGAGAC GAAAAACCCA GTTTACCTGG GAGGCTACCC TGTCGGGGTA 
AAGCAGAACT GCCTGTCAAT CACCACCCGG TTCAGAGGTT GTCTGAAGAA GATACAGCTC 
ATTAAACCGC ACCTGAGCGA TACGCTGGAC CTAAGCTCCG CCCACGTCCT CTCAGGTGTC 
ACTCCTGACT CCTGCCCTGT AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lama1 ( XM_029842713.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029698573.1 Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X2, mRNA.

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CloneID	OTo43638
Clone ID Related Accession (Same CDS sequence)	XM_029842713.1
Accession Version	XM_029842713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	laminin subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGC TGCTGTTGGT GCTGCTGCTG GATGGAGCTG CAGCTCAGCT GAGAGGCCTG 
TTTCCTGCCA TCTTGAACTT GGCCACCAAC GCCGTGGTCG ACAGCAACGC CACGTGCGGC 
GACCCGGACC CGGAGGTCTA CTGTAAACTG GTGGAGCACG TTCCGGGTCG ACCCGTAAAG 
AACCCTCACT GCGCACGCTG TGACGCCGCC TCGCTTCTGC TGAGAGAACG ACATCCCATC 
ACCAACGCCA TCGACGGAAC CAACCGCTGG TGGCAGAGTC CCAGTATCAA GAATGGGCGG 
CAGTTTCACT GGGTCACCAT CACGCTGGAC CTGAAACAGA TCTTCCAGGT CGCCTACGTC 
ATCATCAAAG CAGCGAACTC TCCTCGCCCA GGTAACTGGA TCCTGGAGCG CTCCCTGGAC 
GGGGTGACCT TTGACCCCTG GCAGTATTAC GCAATTAGCA ACTCCGAGTG CTTGTCCCAT 
TACAACGTCA CCCCGAGACT CGGACCTCCG ACCTACAAGA GGGACACGGA GGTCATCTGC 
ACGTCCTACT ACTCCAGACT GAACCCTCTG GAGAACGGAG AGATCCACAC CTCGCTGATC 
AACGGCCGCC CCGGAGCGGA CGACCTGACG CCCGAGCTCT TGGACTTCAC CTCGGCCCGT 
TACATCCGAC TCAGGCTCCA GCGGATCAGG ACGCTCAACG CCGACCTCAT GATGCTGAGC 
ACGCGGGACC CCCGAGACAT CGACCCCATC GTCACACGGA GGTATTATTA CTCCATCAAA 
GATGTGTCCG TGGGTGGAAT GTGCATCTGT TATGGCCACG CCCAGAATTG CCCCTTGGAC 
CCGGTCACAA AGAAACTACA CTGTGTGTGC GAACACAACA CCTGTGGAGA GAACTGTAAC 
GAGTGTTGCC CTGGTTACCA TCAGCAGCCA TGGCAACCAG GAACGGTTCG TGAGGGAAAC 
ACCTGTGAAA AATGTAACTG TCACAACAAA GCAACCGACT GTTTCTACAA TCAGACGGTG 
GCCGACCTCT CTCTGAGTTT GAATATCCAC GGCGTCCGTC GAGGGGGCGG AGTCTGTGTC 
TCCTGTCTAC ACAACACCGC CGGGCTCAAC TGTGAGAGCT GCACTGATGG CTACTACAGA 
CCTGCTGAGG TCTCTCCGTA TTCAGACTTT CCGTGTGTTG CCTGTAACTG TGACCTGAGA 
GGATCAGAGT CACCTGTGTG CTCCAGGGAC GACAAGCATC CGGGTCAGTG TGTGTGTAAG 
GAGGGTTTCG CAGGTGAACG GTGCGACACC TGTGCGTTTG GATACAGAGA CTTTCCCCAG 
TGTGTTCGCT GTGAGTGTGA CGCTGCTGGC AGCACCAACG CGGACCCCTG CAGACCCTGC 
ACCTGCAAGG TGAACGTGAT GGGAGCCCAC TGTGATCTGT GTAAACCCGG ATTCTACAAC 
CTCCAGGAGA AGAATCCGCT CGGCTGCACC GACTGTTTCT GCTTCGGCGT GTCCGACGTC 
TGTGAGAGTT CTGCTTGGTC CACAACTCAG GTGCTTCATA GAGATGCTTG GCTCCTCCCC 
CGCCCGGCTC CTCAGAACAT TACAGTCCTC CAGGACAATG ATTTACCCTC ACCTGGAAAC 
ACTTCCTCTG GCCACTCCCA CCAGGACGTT CTGCTGTGGG ATGCACCTGA CGGTTTCCTT 
GGTAACAAGC TGGGCTCCTA TGGAGGCTTC CTGAACTACT CTCTGCTGTA TGACATTCCA 
TCGGACAATG AGGACCGCTC TATTCCCGCC CACTCTGACG TCATGATCAA GGGAAACGGT 
CGTGCTCTAC ATTCCATCCC TCACTTCCTC TTTGTCTCTC CTCTTGCTGA GAGTGTGGTT 
GCCATAGTGA TGGTTGACGG GCAGTTCAGG GATGTTCAGT CAGGCGACCC CGTGAGCCGC 
GATGAGCTCC TGTCGGCCCT CGCCGACGTT ACGACTCTGA CCGTCAGGGT CGGTCTGAAC 
GCCACCGCAG ATGGACCGAT ACGTCTCAGC TGGATCTCCC TGGACGCCGC TGACCCGGGC 
CCAGGTTCTG CCCTGGACGC CGCTGACCCG GGCCCAGGTT CTGCCCAAGA GGCGGTCGCC 
GTGGAAAAGT GTGAATGTCC GTGGGGTTAC ACCGGCACTT CCTGTGAGAC GTGTCTCCCG 
GGGTTCTACA GAGTGGGCGG GGTTTTGTTC GGAGGAAACT GTATGCAGTG CGAGTGTAAC 
GACCACGCGT CAGAGTGTGA CATCAGTGGC GACTGTTGGG GTTGTACACA TAACACCAGC 
GGTCCTCACT GTGAAGAGTG TCTCCCTGGT TTCTATGGCA ACGCCACAGA GGGGACAGCA 
GATGACTGCC AGTTGTGTCC TTGTCCTCTG ACAAACCCGT CCAACAGTTT CAGTCCCACC 
TGCGTGCTGG AAGGGTCAGG AAAGGTGGTG TGTGACCAGT GCCAGGAGGG ACACACAGGA 
AGCAACTGTG AAAGGTGTGC GAGTGGTTTC TATGGTAACC CACGGGCAGC CAATGGCACC 
TGTGTGCGCT GTGATTGTCA CGGTAATGTG AACGCAAGTG AGGCGGGACA CTGTGACGTC 
ATCACCGGCG AGTGCCTGCG GTGCCTTTTC AACACCGGCG GACGCCACTG CGAGGTCTGT 
CAGCCCGGTT ACTATGGAGA CGCAGTGCTT GCCAAGAACT GTCAGGAATG TGGCTGTGAC 
GTCAACGGCG CCCCCTCCAG TGTGTGTGAC GTCACAACGG GTCAATGTCT TTGCAAGGAA 
AACGTGACAG GACGCACTTG TGACCAGTGT CAGTCGGGCT TTTTCGGCCT CCGCAGTGAG 
ATGGGCTGTC AGCTCTGTGG CTGCAACCAA TCGGGATCGC TGTCTGAGTC ATGTGACGAG 
CAGGGCCGCT GCCCGTGCGT GAAGGGTGTG GGGGGAGACA AGTGTGACCG CTGTGGTCAC 
GGCTACTACG GCGTGGACGT CAGCAGCTGC AGAGCATGCA ACTGTGTCGA CACGGGAGGG 
AACTGTGATC CCCAGAGCGG GGAGTGCATG TGCCCCGCCT TTACAGAGGG ACCCAACTGC 
AACAGATGTG AAACTGGATA CTGGGGTCAC GACCCCACAA CTGGCTGCAC GCCGTGCAGC 
TGCAGCAAGG ACGGAAGCTC CGCCCCTGAC TGTGATGCGA CCAGCGGCCA GTGTTGGTGC 
AGGAAGGGGT TTGGTGGCCG GTCATGTGAC CACTGTGCCG CTGGGCACCA TGGTTACCCG 
TCATGTTTAC CATGCGGCTG TGACCTGGCA GGAACTGATG AAAAGTTCTG CAACACGACG 
CTGAACACGT GTGACTGTCT GGACACGGGA GTGTGTGTGT GCAAGGTGGG CGTGTCTGGG 
CGCCGCTGCG AGGAGTGTGT CGCCGGCCGA TTCGCTCTGT CGGTGGACAA TCCCGATGGC 
TGCTCTCCGT GTTTCTGTTC GGGGGTGAGC GGCGACTGTG AGGAGCAGGG GGGGCTGATC 
CGACTACCGG TCACTCTGGA CCAGTCTCCT GGCCTCTTGT CAGTGGTTAG TCAGTTGAAC 
TTGCAGGGTG TCGTGTCTGG AGTTTACCAG CAGGGCGGCG ACATGCTGCT CGACACCAGG 
CACCTCAACA CCAGTGGCTG GGCTGGCCCA CTCTACTGGA GACTGCCCCC CCAGTTTGAA 
GGCCAACAGC TTCTGTCCTA CGGCGGCTCC TTATCCTACA TCATCACCTT TTATGCTGAG 
GATGGCGCTG GGCTGTCCAA TCAGGAGCCC CAGATCATGA TAAAAGGTGG GACCCTCAAG 
AAGATGGTGA TATACATGGA CGTGGTTGCC CCTAGCAACG GCATTAGGAC AAACCATGAG 
ATCAGGATGA CGGAGCACAA GTGGAAATAC TTTAACTCAG TGTTGGAGAC GTCGGTGGGT 
CGCTCGGACT TCATGTCCGT CCTGGGCAAC ATCGAGTACG TCCTCATCAA AGCGTCTTAC 
GGGAGCAGGC TGCAGCAGAG CAGGATCTCA AATATTACCA TGGAAACGGC GCTGGAGAAC 
GAGGACGGGT CAGAGGTCGG CGGGGACATG GCAAGATCGA TCGAGTCCTG CATCTGTCCC 
ACGGGATACA CCGGACTGTC CTGTCAGGAC TGTGCCGCTG GATTCTTCCG TCAGCCGCTG 
TCCGAGTTGT CACCTCAGAG TCAAAGGTTG ATGTCGGTGC GTCCGTGCGT TCCGTGTCGC 
TGCAACAACC ACAGCGAGAG CTGTGATGCA GCGAGTGGAG AATGTCAGTG TCAGCATCAC 
ACCAGCGGGT GGAGCTGTCA GCAGTGTTCC CAAGGGTATC ACGGGAACGT TAGCGGCTCC 
ATAAGTGACT GCTCTCTTTG CGCCTGCCCC CTGAAGGACA ACAGCTTCAG CCCCACCTGT 
GTGTCGGAGG GAGTGCTGGG AGACTTCCGC TGCACAGCCT GTGTTCCCGG ATACGAGGGG 
AGATACTGTG AAAAGTGTTC CGTCGGTTAC TATGGTAACC CCACTTTGCC AGGCGGCGTG 
TGTTCACGGT GTAACTGTTC TGGGTGGGGT TCTCTTCAGC CGCTGTGTGA CCAGCTGAAT 
GGGAAGTGCG ACTGTAAGGC TGGTGTCAAA GGTCAATCAT GTGACCAGTG CGAGGACAGA 
CACATCCTGC AGGAAGGAGA GTGTGTGTCG TGTGAGGACA ACTGCACCGG TGTGATGCTC 
AACGACCTGG AAGACTTGGA CGACCACCTC GCCGCCGTCA ACATCAGCAG CTTTGTTGTG 
GCCTCGTACC TTCGGTTAGC GGCGCTGGAG AACCAGACCA GAGGCGTCCA GGCGATGGCG 
GCGGAAAACA CTTCAGCCTC TCTGCAGCTG AGCGGCCTCG AAGAGCAACT CGGCCTCATG 
AGCCTCGACC TCGGCGTCTT GCTGCAGCTG GTGGTAAATA TGTCTGGTGA CCTTCAGAGA 
CTGGACACAT CTGCTCAGGA CACCATCCAT CAGGGCTCAC AGCAAATCCA GAACATCAAC 
ATCCTTCAAG ACAAAGTGCA GGCTCTTCAG CAGGAGGCCG AACGCCTGAA TGCGACTGCA 
GAAGAAGAGC TGACGACAGC CAACAGAACA CATCTGCTGC AAGAACTTGA GTTGATGTTG 
AGAAGCATGA GAGTGACCAA CCTCACCGCT GCCAGCAGCC TGGCCAATCA GGAGCTCCTA 
CGCGCCACAT CTGTCCTCTC CCTGCAGTCG GACCTGCTCT CCCGCAGGGC CGCAGTGGAC 
GACGGCCTCC GCCACCTTTC GACCTCCCTC AGCAGTAACA GCGGAACACT GCGCCACCTA 
CAGGCACAAC TGAGCGGCGC CACACAACAA AACATGCAGA CACACGTGCT TCTGGAGGTC 
CTGCACAACC TGACGCAGCA GCACCAGTCT GTCCAGCAGG ACATCAGCCA GGAGCGCCTC 
CTGGTGGCGG GACTGATGCA GGACACTCAG CTCGTGCTGG GCGACAGCTT CAGCATCATG 
GAGGACCTAA CCAACACCAC CTCGCAGGTG GAGGTGTTTG GCAGTTACCT GGATCAGTGG 
AGCCCCCTGC TGAGGAAACG GGTGGATGGT TTGGTTGGTG GTCTGAAGAT GACGGATTCC 
CTGGAGAAGG TCTACCTTTC AGAGAAACAC GCCCAGCAGC TGGACAACGA CGCCCACTCC 
CTGCACAGCT CGCTGCTGTC TGTGTATAAT GCGTCTCAAA ATGGCAGCCA GCTGCTTCAG 
GGGGGGAACA GCGGCGTCCC GGAGCAGGTG GAGGCGCTGC GGCAGGAGGC CCTCATGGCC 
GACCGGTCCG CCCGGCTGCT GTTCAACGCG ACGGTCCAAT CAGAACCGCC CCTGCCAGAG 
GAGAGCAGGA CTCGCCTGAA CATCAGCGCC GCTGTTTTGG AAACCTGTCG ACAGATCGGC 
GCTTCATCCG CAGACTTGCA GATGAACGTC TCCATGGTAA CCAGCAGACT AGGATTATTT 
AAAGAAAGCT TCCAGAAGTT CGGTCTCCTA TTTCATCATT CGATCACAGA GCTGCAGAGC 
CTCTCAAATG GTTCACTGAC CGAGCTGCAA CAAGTTCAGC TGCAGGCTGC AGAAGCAGAC 
TCCAGGCTAC AGGGGGCGCT ACTGAGGCTA CAGAAACTCA GACAGCAGCT CCAGGATTCT 
TCCTCTGCAG TGGAAAACAC CAGCAACACC GTCACAGAGA CCCAGCAGCT CACAGCCCTC 
ACACACTCCA CAGTCGATCA GGCGCGTCAG AAACTGGGCG AGGTGGAGCG TCGCACAGAG 
CGCCTTATCG ACCAGATCAA GCCCCTGAGC ATGCTGGGAG AAACGATAAA CAGGAACCTG 
TCTGACATCA GAGAGCTGAT CAATCAGGCC CGAAGACAGG CCGCCTCGAT TAAGGTGGCG 
GTGCAGGCAG ACAGAAGCTG CGTGCGCTCC TACAGGCCAC AGTCCGAGTC CAGGAACTTC 
AACACGCTGA GTCTTGTCTT GAGGACGTCC AGTCCAAACA ACCTGCTGTT CTTCCAGGGC 
AGCAACACCA CGGTGGACTT CCTGGCTCTG GAGATGTATC GTGGGAAGGT GTCTCTGCTG 
TGGGATCTGG GTTCAGGTAG CACCAGACTG GAGTTTCCCG GACTGGACAT CACCAACAAC 
AGATGGACCA GAATCAACGC CACGCGGTTT GGGGCTCATG CGTCTCTGTG GGTTCATCAG 
CTGGAGTCGG ACTCGGAGCC CCTCCCTGCA GTAACGGCCA CCTCCCCGGG TCCCGCCAGG 
ATTTTAGACA CGGACAGAAA CTCTTGGCTC TACATTGGTG GACTGGGAGC TCACGGACAG 
AGGCCGGCAG CCCTGCGCTC CACCACATTC CAGGGATGTC TGGGCGAAGC GGCCTTGAAC 
GAGCGGAACA TCGGCCTCTG GAACTACCAC AGAAGGGAGG GAGAGTGTGG AGGCTGCTTC 
AGCAGTCCAC AAACGGAGGA GACCTCCTTC CATTTCGACG GATCCGGATT CTCTTTGGTC 
CAGAAGCCTC TACGGGCGAC CTCCACCTCC ATCGTCTTGC TGTTCAAAAC ACTGTCACCA 
GGGGGGTTAC TGTTGTACCT GGCCTCCAAC AACACGAGGG ACTTTCTGTC CATCGAGCTA 
GTAGAGGGGC AGGTTCGCCT GACCTTTGAC CTCGGCTCAG GTGCACTCGT CCTGACCTCC 
AGCAGGAAGT ACAACACTGG CGTCTGGTAC AAGATCACGC TGCAGAGGAA CAAACGTAAA 
GGTTACCTTT CCATCATGGC TGCTGACCAG TCGTCGGAAA AGGAGGTGTT GGAGGCGGAG 
TCTCCCGGGC TGGCGTCCGA CCTTAACCGC TCCGACTTTG ACCCCATCTA CATCGGAGGA 
CTTCCTGCCT CCAGACCCAT CAGGCGACAG GTGGTGTCCA GGGCTTACGT GGGCTGTATA 
AAAAATGTGG AGATCGCCCG GTCCAACTTT GACCTGCTGA GAGACGCTTT TGGCGTCAGG 
AAGGGCTGCG TTCTTGAGGC CCTCAGGAGC GTGTCGCTGT TGACCGGCGG CTTCGTTCAA 
ATGGCTCCTC GAGCTCTTGG ACCAGAGGTG GAGCTTCTCT TTTCCTTCTC CACCAACAAC 
CGGTCAGGAA TTCTGCTCGC TGCCGTCACC GACGACCACG TTCAGAGGCG GGTAGGGCGG 
AAAGTCCGCT TCCTGTCCAT CCACCTGGCC TCAGGTGCGG TGGAGGCCGA CATTGGTGAA 
GTGGGAGGAG CCACACAAAC CGTGACAGTG GTGAAACCTA AGGGAGGATT TTTTAGCGAT 
GGTCTGAAAC ACTCTGTCAT CGTTACCGTC AACAAGAAGA CCCTGTCCAT ACAGGTGGAT 
GAGACACACC TGAAGTCCAT CCCTCTGTTG CCGGGCAGAT TTTCTAATTT GTCTCCAGCC 
TCATTTTTTA TTGGTGGAGT ACCTGTAGGG GCGGGGCTCC AGCTGCCCTC CAGGCTGCGG 
CAGATTTCTT TATCCTTCAG AGGCTGCATC CAACACCTGG TGTTAGGAGG AGGAGCGCTC 
GTTGACCTCT CAGGAGCGTT GAGGTATGAG GGGGTGACCC TGGACAGCTG CCTGCTGGAA 
GAGGCGGCCA GGGGGGCGGT GCTTCCTGAT GACCCGGACA CAGAACCAAC CATGGACCCG 
GTCCGCCTCC CTGTGGTACC GCCCACCCCG CTGGGTGTCC TGCCCCCAGG CGCTCTAACG 
TGTGCAGCAC AGGTGGACTC CTCCTTCCTG CCCTCCGCAG CTCAGTTCGG TTTGTCCAAA 
CACAGTCACA TGACGTTCCT GATCAACCCC GGCGCCCTCA GGAAAAGTGT GTCTCTGAAG 
ATATCGGCTC GGACCCGATC TCCGGACGGC CTGCTCCTCC TCCTCTCCGA CGCCAAACAG 
ATGGACTTCG CCGTCGTCAA ACTAGCGGGG GGGAAGGTGA CGTTGTCAGC CGACCTGGGG 
AAGGGTCCCA CCTCCGTCAC CTCACCTGTG TCCATCACTG ACGGACTCTG GCACACTGTG 
AGCGCCGAGG TCAGCCGCCG CTCGCTCGTC CTGGCGGTCG ACGGTTCAAA GCCCGGCTCG 
GCGTCCATCA GGGGGAACCA GCTGGACGTG GACAGGCGCC TGTACCTGGG GGGGCTGCCG 
CACGCACACG CCACCAGGAG GATTAACGTC AGCAGCAGCT TCCAGGGCTG CGTTCGGTCC 
GTCCACCTGA ACGGCGCCGT GTTGGATCTG ACCAAACCGG CCTCGCGCCA CAACGTCACT 
TCCTGTTTCA CCGATGACCA GGCAGGAAGC TACTTCGACG GCAGCGGACA CGCCCTCTTC 
ATGCGCGATG GCTATAAAGT CGGATCCGAT GTTTCTGTAT CTCTGGAGTT CCGGACCAGC 
CAATCAGAAG GAGTGTTTTT AGGAATTAGC AGTGCCAAAA TTGATGCCAT CGGACTCGAG 
ATGATCAAAG GACAGGTGGT GTTTAACGTG AACAATGGCG CCGGGAGAGT GACGGTGCGC 
TCCGTCGGTC CGATGCTGTG TGACGGCCGA TGGCACCGCG TCCTGGCCAG GAAAACCAAA 
CACAGCCTGA ATTTACAGGT GGATGGGCGG AGCTACATCA CCCCCAACCC TTACCCACAA 
TCCACCTCGG CTGAGACGAA AAACCCAGTT TACCTGGGAG GCTACCCTGT CGGGGTAAAG 
CAGAACTGCC TGTCAATCAC CACCCGGTTC AGAGGTTGTC TGAAGAAGAT ACAGCTCATT 
AAACCGCACC TGAGCGATAC GCTGGACCTA AGCTCCGCCC ACGTCCTCTC AGGTGTCACT 
CCTGACTCCT GCCCTGTAGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029698573.1
CDS	90..9353
Translation	MKLLLLVLLLDGAAAQLRGLFPAILNLATNAVVDSNATCGDPDPEVYCKLVEHVPGRPVKNPHCARCDAASLLLRERHPITNAIDGTNRWWQSPSIKNGRQFHWVTITLDLKQIFQVAYVIIKAANSPRPGNWILERSLDGVTFDPWQYYAISNSECLSHYNVTPRLGPPTYKRDTEVICTSYYSRLNPLENGEIHTSLINGRPGADDLTPELLDFTSARYIRLRLQRIRTLNADLMMLSTRDPRDIDPIVTRRYYYSIKDVSVGGMCICYGHAQNCPLDPVTKKLHCVCEHNTCGENCNECCPGYHQQPWQPGTVREGNTCEKCNCHNKATDCFYNQTVADLSLSLNIHGVRRGGGVCVSCLHNTAGLNCESCTDGYYRPAEVSPYSDFPCVACNCDLRGSESPVCSRDDKHPGQCVCKEGFAGERCDTCAFGYRDFPQCVRCECDAAGSTNADPCRPCTCKVNVMGAHCDLCKPGFYNLQEKNPLGCTDCFCFGVSDVCESSAWSTTQVLHRDAWLLPRPAPQNITVLQDNDLPSPGNTSSGHSHQDVLLWDAPDGFLGNKLGSYGGFLNYSLLYDIPSDNEDRSIPAHSDVMIKGNGRALHSIPHFLFVSPLAESVVAIVMVDGQFRDVQSGDPVSRDELLSALADVTTLTVRVGLNATADGPIRLSWISLDAADPGPGSALDAADPGPGSAQEAVAVEKCECPWGYTGTSCETCLPGFYRVGGVLFGGNCMQCECNDHASECDISGDCWGCTHNTSGPHCEECLPGFYGNATEGTADDCQLCPCPLTNPSNSFSPTCVLEGSGKVVCDQCQEGHTGSNCERCASGFYGNPRAANGTCVRCDCHGNVNASEAGHCDVITGECLRCLFNTGGRHCEVCQPGYYGDAVLAKNCQECGCDVNGAPSSVCDVTTGQCLCKENVTGRTCDQCQSGFFGLRSEMGCQLCGCNQSGSLSESCDEQGRCPCVKGVGGDKCDRCGHGYYGVDVSSCRACNCVDTGGNCDPQSGECMCPAFTEGPNCNRCETGYWGHDPTTGCTPCSCSKDGSSAPDCDATSGQCWCRKGFGGRSCDHCAAGHHGYPSCLPCGCDLAGTDEKFCNTTLNTCDCLDTGVCVCKVGVSGRRCEECVAGRFALSVDNPDGCSPCFCSGVSGDCEEQGGLIRLPVTLDQSPGLLSVVSQLNLQGVVSGVYQQGGDMLLDTRHLNTSGWAGPLYWRLPPQFEGQQLLSYGGSLSYIITFYAEDGAGLSNQEPQIMIKGGTLKKMVIYMDVVAPSNGIRTNHEIRMTEHKWKYFNSVLETSVGRSDFMSVLGNIEYVLIKASYGSRLQQSRISNITMETALENEDGSEVGGDMARSIESCICPTGYTGLSCQDCAAGFFRQPLSELSPQSQRLMSVRPCVPCRCNNHSESCDAASGECQCQHHTSGWSCQQCSQGYHGNVSGSISDCSLCACPLKDNSFSPTCVSEGVLGDFRCTACVPGYEGRYCEKCSVGYYGNPTLPGGVCSRCNCSGWGSLQPLCDQLNGKCDCKAGVKGQSCDQCEDRHILQEGECVSCEDNCTGVMLNDLEDLDDHLAAVNISSFVVASYLRLAALENQTRGVQAMAAENTSASLQLSGLEEQLGLMSLDLGVLLQLVVNMSGDLQRLDTSAQDTIHQGSQQIQNINILQDKVQALQQEAERLNATAEEELTTANRTHLLQELELMLRSMRVTNLTAASSLANQELLRATSVLSLQSDLLSRRAAVDDGLRHLSTSLSSNSGTLRHLQAQLSGATQQNMQTHVLLEVLHNLTQQHQSVQQDISQERLLVAGLMQDTQLVLGDSFSIMEDLTNTTSQVEVFGSYLDQWSPLLRKRVDGLVGGLKMTDSLEKVYLSEKHAQQLDNDAHSLHSSLLSVYNASQNGSQLLQGGNSGVPEQVEALRQEALMADRSARLLFNATVQSEPPLPEESRTRLNISAAVLETCRQIGASSADLQMNVSMVTSRLGLFKESFQKFGLLFHHSITELQSLSNGSLTELQQVQLQAAEADSRLQGALLRLQKLRQQLQDSSSAVENTSNTVTETQQLTALTHSTVDQARQKLGEVERRTERLIDQIKPLSMLGETINRNLSDIRELINQARRQAASIKVAVQADRSCVRSYRPQSESRNFNTLSLVLRTSSPNNLLFFQGSNTTVDFLALEMYRGKVSLLWDLGSGSTRLEFPGLDITNNRWTRINATRFGAHASLWVHQLESDSEPLPAVTATSPGPARILDTDRNSWLYIGGLGAHGQRPAALRSTTFQGCLGEAALNERNIGLWNYHRREGECGGCFSSPQTEETSFHFDGSGFSLVQKPLRATSTSIVLLFKTLSPGGLLLYLASNNTRDFLSIELVEGQVRLTFDLGSGALVLTSSRKYNTGVWYKITLQRNKRKGYLSIMAADQSSEKEVLEAESPGLASDLNRSDFDPIYIGGLPASRPIRRQVVSRAYVGCIKNVEIARSNFDLLRDAFGVRKGCVLEALRSVSLLTGGFVQMAPRALGPEVELLFSFSTNNRSGILLAAVTDDHVQRRVGRKVRFLSIHLASGAVEADIGEVGGATQTVTVVKPKGGFFSDGLKHSVIVTVNKKTLSIQVDETHLKSIPLLPGRFSNLSPASFFIGGVPVGAGLQLPSRLRQISLSFRGCIQHLVLGGGALVDLSGALRYEGVTLDSCLLEEAARGAVLPDDPDTEPTMDPVRLPVVPPTPLGVLPPGALTCAAQVDSSFLPSAAQFGLSKHSHMTFLINPGALRKSVSLKISARTRSPDGLLLLLSDAKQMDFAVVKLAGGKVTLSADLGKGPTSVTSPVSITDGLWHTVSAEVSRRSLVLAVDGSKPGSASIRGNQLDVDRRLYLGGLPHAHATRRINVSSSFQGCVRSVHLNGAVLDLTKPASRHNVTSCFTDDQAGSYFDGSGHALFMRDGYKVGSDVSVSLEFRTSQSEGVFLGISSAKIDAIGLEMIKGQVVFNVNNGAGRVTVRSVGPMLCDGRWHRVLARKTKHSLNLQVDGRSYITPNPYPQSTSAETKNPVYLGGYPVGVKQNCLSITTRFRGCLKKIQLIKPHLSDTLDLSSAHVLSGVTPDSCPVG

Target ORF information:

RefSeq Version	XM_029842713.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029842713.1

ORF Insert Sequence:

ATGAAGTTGC TGCTGTTGGT GCTGCTGCTG GATGGAGCTG CAGCTCAGCT GAGAGGCCTG 
TTTCCTGCCA TCTTGAACTT GGCCACCAAC GCCGTGGTCG ACAGCAACGC CACGTGCGGC 
GACCCGGACC CGGAGGTCTA CTGTAAACTG GTGGAGCACG TTCCGGGTCG ACCCGTAAAG 
AACCCTCACT GCGCACGCTG TGACGCCGCC TCGCTTCTGC TGAGAGAACG ACATCCCATC 
ACCAACGCCA TCGACGGAAC CAACCGCTGG TGGCAGAGTC CCAGTATCAA GAATGGGCGG 
CAGTTTCACT GGGTCACCAT CACGCTGGAC CTGAAACAGA TCTTCCAGGT CGCCTACGTC 
ATCATCAAAG CAGCGAACTC TCCTCGCCCA GGTAACTGGA TCCTGGAGCG CTCCCTGGAC 
GGGGTGACCT TTGACCCCTG GCAGTATTAC GCAATTAGCA ACTCCGAGTG CTTGTCCCAT 
TACAACGTCA CCCCGAGACT CGGACCTCCG ACCTACAAGA GGGACACGGA GGTCATCTGC 
ACGTCCTACT ACTCCAGACT GAACCCTCTG GAGAACGGAG AGATCCACAC CTCGCTGATC 
AACGGCCGCC CCGGAGCGGA CGACCTGACG CCCGAGCTCT TGGACTTCAC CTCGGCCCGT 
TACATCCGAC TCAGGCTCCA GCGGATCAGG ACGCTCAACG CCGACCTCAT GATGCTGAGC 
ACGCGGGACC CCCGAGACAT CGACCCCATC GTCACACGGA GGTATTATTA CTCCATCAAA 
GATGTGTCCG TGGGTGGAAT GTGCATCTGT TATGGCCACG CCCAGAATTG CCCCTTGGAC 
CCGGTCACAA AGAAACTACA CTGTGTGTGC GAACACAACA CCTGTGGAGA GAACTGTAAC 
GAGTGTTGCC CTGGTTACCA TCAGCAGCCA TGGCAACCAG GAACGGTTCG TGAGGGAAAC 
ACCTGTGAAA AATGTAACTG TCACAACAAA GCAACCGACT GTTTCTACAA TCAGACGGTG 
GCCGACCTCT CTCTGAGTTT GAATATCCAC GGCGTCCGTC GAGGGGGCGG AGTCTGTGTC 
TCCTGTCTAC ACAACACCGC CGGGCTCAAC TGTGAGAGCT GCACTGATGG CTACTACAGA 
CCTGCTGAGG TCTCTCCGTA TTCAGACTTT CCGTGTGTTG CCTGTAACTG TGACCTGAGA 
GGATCAGAGT CACCTGTGTG CTCCAGGGAC GACAAGCATC CGGGTCAGTG TGTGTGTAAG 
GAGGGTTTCG CAGGTGAACG GTGCGACACC TGTGCGTTTG GATACAGAGA CTTTCCCCAG 
TGTGTTCGCT GTGAGTGTGA CGCTGCTGGC AGCACCAACG CGGACCCCTG CAGACCCTGC 
ACCTGCAAGG TGAACGTGAT GGGAGCCCAC TGTGATCTGT GTAAACCCGG ATTCTACAAC 
CTCCAGGAGA AGAATCCGCT CGGCTGCACC GACTGTTTCT GCTTCGGCGT GTCCGACGTC 
TGTGAGAGTT CTGCTTGGTC CACAACTCAG GTGCTTCATA GAGATGCTTG GCTCCTCCCC 
CGCCCGGCTC CTCAGAACAT TACAGTCCTC CAGGACAATG ATTTACCCTC ACCTGGAAAC 
ACTTCCTCTG GCCACTCCCA CCAGGACGTT CTGCTGTGGG ATGCACCTGA CGGTTTCCTT 
GGTAACAAGC TGGGCTCCTA TGGAGGCTTC CTGAACTACT CTCTGCTGTA TGACATTCCA 
TCGGACAATG AGGACCGCTC TATTCCCGCC CACTCTGACG TCATGATCAA GGGAAACGGT 
CGTGCTCTAC ATTCCATCCC TCACTTCCTC TTTGTCTCTC CTCTTGCTGA GAGTGTGGTT 
GCCATAGTGA TGGTTGACGG GCAGTTCAGG GATGTTCAGT CAGGCGACCC CGTGAGCCGC 
GATGAGCTCC TGTCGGCCCT CGCCGACGTT ACGACTCTGA CCGTCAGGGT CGGTCTGAAC 
GCCACCGCAG ATGGACCGAT ACGTCTCAGC TGGATCTCCC TGGACGCCGC TGACCCGGGC 
CCAGGTTCTG CCCTGGACGC CGCTGACCCG GGCCCAGGTT CTGCCCAAGA GGCGGTCGCC 
GTGGAAAAGT GTGAATGTCC GTGGGGTTAC ACCGGCACTT CCTGTGAGAC GTGTCTCCCG 
GGGTTCTACA GAGTGGGCGG GGTTTTGTTC GGAGGAAACT GTATGCAGTG CGAGTGTAAC 
GACCACGCGT CAGAGTGTGA CATCAGTGGC GACTGTTGGG GTTGTACACA TAACACCAGC 
GGTCCTCACT GTGAAGAGTG TCTCCCTGGT TTCTATGGCA ACGCCACAGA GGGGACAGCA 
GATGACTGCC AGTTGTGTCC TTGTCCTCTG ACAAACCCGT CCAACAGTTT CAGTCCCACC 
TGCGTGCTGG AAGGGTCAGG AAAGGTGGTG TGTGACCAGT GCCAGGAGGG ACACACAGGA 
AGCAACTGTG AAAGGTGTGC GAGTGGTTTC TATGGTAACC CACGGGCAGC CAATGGCACC 
TGTGTGCGCT GTGATTGTCA CGGTAATGTG AACGCAAGTG AGGCGGGACA CTGTGACGTC 
ATCACCGGCG AGTGCCTGCG GTGCCTTTTC AACACCGGCG GACGCCACTG CGAGGTCTGT 
CAGCCCGGTT ACTATGGAGA CGCAGTGCTT GCCAAGAACT GTCAGGAATG TGGCTGTGAC 
GTCAACGGCG CCCCCTCCAG TGTGTGTGAC GTCACAACGG GTCAATGTCT TTGCAAGGAA 
AACGTGACAG GACGCACTTG TGACCAGTGT CAGTCGGGCT TTTTCGGCCT CCGCAGTGAG 
ATGGGCTGTC AGCTCTGTGG CTGCAACCAA TCGGGATCGC TGTCTGAGTC ATGTGACGAG 
CAGGGCCGCT GCCCGTGCGT GAAGGGTGTG GGGGGAGACA AGTGTGACCG CTGTGGTCAC 
GGCTACTACG GCGTGGACGT CAGCAGCTGC AGAGCATGCA ACTGTGTCGA CACGGGAGGG 
AACTGTGATC CCCAGAGCGG GGAGTGCATG TGCCCCGCCT TTACAGAGGG ACCCAACTGC 
AACAGATGTG AAACTGGATA CTGGGGTCAC GACCCCACAA CTGGCTGCAC GCCGTGCAGC 
TGCAGCAAGG ACGGAAGCTC CGCCCCTGAC TGTGATGCGA CCAGCGGCCA GTGTTGGTGC 
AGGAAGGGGT TTGGTGGCCG GTCATGTGAC CACTGTGCCG CTGGGCACCA TGGTTACCCG 
TCATGTTTAC CATGCGGCTG TGACCTGGCA GGAACTGATG AAAAGTTCTG CAACACGACG 
CTGAACACGT GTGACTGTCT GGACACGGGA GTGTGTGTGT GCAAGGTGGG CGTGTCTGGG 
CGCCGCTGCG AGGAGTGTGT CGCCGGCCGA TTCGCTCTGT CGGTGGACAA TCCCGATGGC 
TGCTCTCCGT GTTTCTGTTC GGGGGTGAGC GGCGACTGTG AGGAGCAGGG GGGGCTGATC 
CGACTACCGG TCACTCTGGA CCAGTCTCCT GGCCTCTTGT CAGTGGTTAG TCAGTTGAAC 
TTGCAGGGTG TCGTGTCTGG AGTTTACCAG CAGGGCGGCG ACATGCTGCT CGACACCAGG 
CACCTCAACA CCAGTGGCTG GGCTGGCCCA CTCTACTGGA GACTGCCCCC CCAGTTTGAA 
GGCCAACAGC TTCTGTCCTA CGGCGGCTCC TTATCCTACA TCATCACCTT TTATGCTGAG 
GATGGCGCTG GGCTGTCCAA TCAGGAGCCC CAGATCATGA TAAAAGGTGG GACCCTCAAG 
AAGATGGTGA TATACATGGA CGTGGTTGCC CCTAGCAACG GCATTAGGAC AAACCATGAG 
ATCAGGATGA CGGAGCACAA GTGGAAATAC TTTAACTCAG TGTTGGAGAC GTCGGTGGGT 
CGCTCGGACT TCATGTCCGT CCTGGGCAAC ATCGAGTACG TCCTCATCAA AGCGTCTTAC 
GGGAGCAGGC TGCAGCAGAG CAGGATCTCA AATATTACCA TGGAAACGGC GCTGGAGAAC 
GAGGACGGGT CAGAGGTCGG CGGGGACATG GCAAGATCGA TCGAGTCCTG CATCTGTCCC 
ACGGGATACA CCGGACTGTC CTGTCAGGAC TGTGCCGCTG GATTCTTCCG TCAGCCGCTG 
TCCGAGTTGT CACCTCAGAG TCAAAGGTTG ATGTCGGTGC GTCCGTGCGT TCCGTGTCGC 
TGCAACAACC ACAGCGAGAG CTGTGATGCA GCGAGTGGAG AATGTCAGTG TCAGCATCAC 
ACCAGCGGGT GGAGCTGTCA GCAGTGTTCC CAAGGGTATC ACGGGAACGT TAGCGGCTCC 
ATAAGTGACT GCTCTCTTTG CGCCTGCCCC CTGAAGGACA ACAGCTTCAG CCCCACCTGT 
GTGTCGGAGG GAGTGCTGGG AGACTTCCGC TGCACAGCCT GTGTTCCCGG ATACGAGGGG 
AGATACTGTG AAAAGTGTTC CGTCGGTTAC TATGGTAACC CCACTTTGCC AGGCGGCGTG 
TGTTCACGGT GTAACTGTTC TGGGTGGGGT TCTCTTCAGC CGCTGTGTGA CCAGCTGAAT 
GGGAAGTGCG ACTGTAAGGC TGGTGTCAAA GGTCAATCAT GTGACCAGTG CGAGGACAGA 
CACATCCTGC AGGAAGGAGA GTGTGTGTCG TGTGAGGACA ACTGCACCGG TGTGATGCTC 
AACGACCTGG AAGACTTGGA CGACCACCTC GCCGCCGTCA ACATCAGCAG CTTTGTTGTG 
GCCTCGTACC TTCGGTTAGC GGCGCTGGAG AACCAGACCA GAGGCGTCCA GGCGATGGCG 
GCGGAAAACA CTTCAGCCTC TCTGCAGCTG AGCGGCCTCG AAGAGCAACT CGGCCTCATG 
AGCCTCGACC TCGGCGTCTT GCTGCAGCTG GTGGTAAATA TGTCTGGTGA CCTTCAGAGA 
CTGGACACAT CTGCTCAGGA CACCATCCAT CAGGGCTCAC AGCAAATCCA GAACATCAAC 
ATCCTTCAAG ACAAAGTGCA GGCTCTTCAG CAGGAGGCCG AACGCCTGAA TGCGACTGCA 
GAAGAAGAGC TGACGACAGC CAACAGAACA CATCTGCTGC AAGAACTTGA GTTGATGTTG 
AGAAGCATGA GAGTGACCAA CCTCACCGCT GCCAGCAGCC TGGCCAATCA GGAGCTCCTA 
CGCGCCACAT CTGTCCTCTC CCTGCAGTCG GACCTGCTCT CCCGCAGGGC CGCAGTGGAC 
GACGGCCTCC GCCACCTTTC GACCTCCCTC AGCAGTAACA GCGGAACACT GCGCCACCTA 
CAGGCACAAC TGAGCGGCGC CACACAACAA AACATGCAGA CACACGTGCT TCTGGAGGTC 
CTGCACAACC TGACGCAGCA GCACCAGTCT GTCCAGCAGG ACATCAGCCA GGAGCGCCTC 
CTGGTGGCGG GACTGATGCA GGACACTCAG CTCGTGCTGG GCGACAGCTT CAGCATCATG 
GAGGACCTAA CCAACACCAC CTCGCAGGTG GAGGTGTTTG GCAGTTACCT GGATCAGTGG 
AGCCCCCTGC TGAGGAAACG GGTGGATGGT TTGGTTGGTG GTCTGAAGAT GACGGATTCC 
CTGGAGAAGG TCTACCTTTC AGAGAAACAC GCCCAGCAGC TGGACAACGA CGCCCACTCC 
CTGCACAGCT CGCTGCTGTC TGTGTATAAT GCGTCTCAAA ATGGCAGCCA GCTGCTTCAG 
GGGGGGAACA GCGGCGTCCC GGAGCAGGTG GAGGCGCTGC GGCAGGAGGC CCTCATGGCC 
GACCGGTCCG CCCGGCTGCT GTTCAACGCG ACGGTCCAAT CAGAACCGCC CCTGCCAGAG 
GAGAGCAGGA CTCGCCTGAA CATCAGCGCC GCTGTTTTGG AAACCTGTCG ACAGATCGGC 
GCTTCATCCG CAGACTTGCA GATGAACGTC TCCATGGTAA CCAGCAGACT AGGATTATTT 
AAAGAAAGCT TCCAGAAGTT CGGTCTCCTA TTTCATCATT CGATCACAGA GCTGCAGAGC 
CTCTCAAATG GTTCACTGAC CGAGCTGCAA CAAGTTCAGC TGCAGGCTGC AGAAGCAGAC 
TCCAGGCTAC AGGGGGCGCT ACTGAGGCTA CAGAAACTCA GACAGCAGCT CCAGGATTCT 
TCCTCTGCAG TGGAAAACAC CAGCAACACC GTCACAGAGA CCCAGCAGCT CACAGCCCTC 
ACACACTCCA CAGTCGATCA GGCGCGTCAG AAACTGGGCG AGGTGGAGCG TCGCACAGAG 
CGCCTTATCG ACCAGATCAA GCCCCTGAGC ATGCTGGGAG AAACGATAAA CAGGAACCTG 
TCTGACATCA GAGAGCTGAT CAATCAGGCC CGAAGACAGG CCGCCTCGAT TAAGGTGGCG 
GTGCAGGCAG ACAGAAGCTG CGTGCGCTCC TACAGGCCAC AGTCCGAGTC CAGGAACTTC 
AACACGCTGA GTCTTGTCTT GAGGACGTCC AGTCCAAACA ACCTGCTGTT CTTCCAGGGC 
AGCAACACCA CGGTGGACTT CCTGGCTCTG GAGATGTATC GTGGGAAGGT GTCTCTGCTG 
TGGGATCTGG GTTCAGGTAG CACCAGACTG GAGTTTCCCG GACTGGACAT CACCAACAAC 
AGATGGACCA GAATCAACGC CACGCGGTTT GGGGCTCATG CGTCTCTGTG GGTTCATCAG 
CTGGAGTCGG ACTCGGAGCC CCTCCCTGCA GTAACGGCCA CCTCCCCGGG TCCCGCCAGG 
ATTTTAGACA CGGACAGAAA CTCTTGGCTC TACATTGGTG GACTGGGAGC TCACGGACAG 
AGGCCGGCAG CCCTGCGCTC CACCACATTC CAGGGATGTC TGGGCGAAGC GGCCTTGAAC 
GAGCGGAACA TCGGCCTCTG GAACTACCAC AGAAGGGAGG GAGAGTGTGG AGGCTGCTTC 
AGCAGTCCAC AAACGGAGGA GACCTCCTTC CATTTCGACG GATCCGGATT CTCTTTGGTC 
CAGAAGCCTC TACGGGCGAC CTCCACCTCC ATCGTCTTGC TGTTCAAAAC ACTGTCACCA 
GGGGGGTTAC TGTTGTACCT GGCCTCCAAC AACACGAGGG ACTTTCTGTC CATCGAGCTA 
GTAGAGGGGC AGGTTCGCCT GACCTTTGAC CTCGGCTCAG GTGCACTCGT CCTGACCTCC 
AGCAGGAAGT ACAACACTGG CGTCTGGTAC AAGATCACGC TGCAGAGGAA CAAACGTAAA 
GGTTACCTTT CCATCATGGC TGCTGACCAG TCGTCGGAAA AGGAGGTGTT GGAGGCGGAG 
TCTCCCGGGC TGGCGTCCGA CCTTAACCGC TCCGACTTTG ACCCCATCTA CATCGGAGGA 
CTTCCTGCCT CCAGACCCAT CAGGCGACAG GTGGTGTCCA GGGCTTACGT GGGCTGTATA 
AAAAATGTGG AGATCGCCCG GTCCAACTTT GACCTGCTGA GAGACGCTTT TGGCGTCAGG 
AAGGGCTGCG TTCTTGAGGC CCTCAGGAGC GTGTCGCTGT TGACCGGCGG CTTCGTTCAA 
ATGGCTCCTC GAGCTCTTGG ACCAGAGGTG GAGCTTCTCT TTTCCTTCTC CACCAACAAC 
CGGTCAGGAA TTCTGCTCGC TGCCGTCACC GACGACCACG TTCAGAGGCG GGTAGGGCGG 
AAAGTCCGCT TCCTGTCCAT CCACCTGGCC TCAGGTGCGG TGGAGGCCGA CATTGGTGAA 
GTGGGAGGAG CCACACAAAC CGTGACAGTG GTGAAACCTA AGGGAGGATT TTTTAGCGAT 
GGTCTGAAAC ACTCTGTCAT CGTTACCGTC AACAAGAAGA CCCTGTCCAT ACAGGTGGAT 
GAGACACACC TGAAGTCCAT CCCTCTGTTG CCGGGCAGAT TTTCTAATTT GTCTCCAGCC 
TCATTTTTTA TTGGTGGAGT ACCTGTAGGG GCGGGGCTCC AGCTGCCCTC CAGGCTGCGG 
CAGATTTCTT TATCCTTCAG AGGCTGCATC CAACACCTGG TGTTAGGAGG AGGAGCGCTC 
GTTGACCTCT CAGGAGCGTT GAGGTATGAG GGGGTGACCC TGGACAGCTG CCTGCTGGAA 
GAGGCGGCCA GGGGGGCGGT GCTTCCTGAT GACCCGGACA CAGAACCAAC CATGGACCCG 
GTCCGCCTCC CTGTGGTACC GCCCACCCCG CTGGGTGTCC TGCCCCCAGG CGCTCTAACG 
TGTGCAGCAC AGGTGGACTC CTCCTTCCTG CCCTCCGCAG CTCAGTTCGG TTTGTCCAAA 
CACAGTCACA TGACGTTCCT GATCAACCCC GGCGCCCTCA GGAAAAGTGT GTCTCTGAAG 
ATATCGGCTC GGACCCGATC TCCGGACGGC CTGCTCCTCC TCCTCTCCGA CGCCAAACAG 
ATGGACTTCG CCGTCGTCAA ACTAGCGGGG GGGAAGGTGA CGTTGTCAGC CGACCTGGGG 
AAGGGTCCCA CCTCCGTCAC CTCACCTGTG TCCATCACTG ACGGACTCTG GCACACTGTG 
AGCGCCGAGG TCAGCCGCCG CTCGCTCGTC CTGGCGGTCG ACGGTTCAAA GCCCGGCTCG 
GCGTCCATCA GGGGGAACCA GCTGGACGTG GACAGGCGCC TGTACCTGGG GGGGCTGCCG 
CACGCACACG CCACCAGGAG GATTAACGTC AGCAGCAGCT TCCAGGGCTG CGTTCGGTCC 
GTCCACCTGA ACGGCGCCGT GTTGGATCTG ACCAAACCGG CCTCGCGCCA CAACGTCACT 
TCCTGTTTCA CCGATGACCA GGCAGGAAGC TACTTCGACG GCAGCGGACA CGCCCTCTTC 
ATGCGCGATG GCTATAAAGT CGGATCCGAT GTTTCTGTAT CTCTGGAGTT CCGGACCAGC 
CAATCAGAAG GAGTGTTTTT AGGAATTAGC AGTGCCAAAA TTGATGCCAT CGGACTCGAG 
ATGATCAAAG GACAGGTGGT GTTTAACGTG AACAATGGCG CCGGGAGAGT GACGGTGCGC 
TCCGTCGGTC CGATGCTGTG TGACGGCCGA TGGCACCGCG TCCTGGCCAG GAAAACCAAA 
CACAGCCTGA ATTTACAGGT GGATGGGCGG AGCTACATCA CCCCCAACCC TTACCCACAA 
TCCACCTCGG CTGAGACGAA AAACCCAGTT TACCTGGGAG GCTACCCTGT CGGGGTAAAG 
CAGAACTGCC TGTCAATCAC CACCCGGTTC AGAGGTTGTC TGAAGAAGAT ACAGCTCATT 
AAACCGCACC TGAGCGATAC GCTGGACCTA AGCTCCGCCC ACGTCCTCTC AGGTGTCACT 
CCTGACTCCT GCCCTGTAGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lama1 ( XM_029842714.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029698574.1 Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X3, mRNA.

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CloneID	OTo43639
Clone ID Related Accession (Same CDS sequence)	XM_029842714.1
Accession Version	XM_029842714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	laminin subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGC TGCTGTTGGT GCTGCTGCTG GATGGAGCTG CAGCTCAGCT GAGAGGCCTG 
TTTCCTGCCA TCTTGAACTT GGCCACCAAC GCCGTGGTCG ACAGCAACGC CACGTGCGGC 
GACCCGGACC CGGAGGTCTA CTGTAAACTG GTGGAGCACG TTCCGGGTCG ACCCGTAAAG 
AACCCTCACT GCGCACGCTG TGACGCCGCC TCGCTTCTGC TGAGAGAACG ACATCCCATC 
ACCAACGCCA TCGACGGAAC CAACCGCTGG TGGCAGAGTC CCAGTATCAA GAATGGGCGG 
CAGTTTCACT GGGTCACCAT CACGCTGGAC CTGAAACAGA TCTTCCAGGT CGCCTACGTC 
ATCATCAAAG CAGCGAACTC TCCTCGCCCA GGTAACTGGA TCCTGGAGCG CTCCCTGGAC 
GGGGTGACCT TTGACCCCTG GCAGTATTAC GCAATTAGCA ACTCCGAGTG CTTGTCCCAT 
TACAACGTCA CCCCGAGACT CGGACCTCCG ACCTACAAGA GGGACACGGA GGTCATCTGC 
ACGTCCTACT ACTCCAGACT GAACCCTCTG GAGAACGGAG AGATCCACAC CTCGCTGATC 
AACGGCCGCC CCGGAGCGGA CGACCTGACG CCCGAGCTCT TGGACTTCAC CTCGGCCCGT 
TACATCCGAC TCAGGCTCCA GCGGATCAGG ACGCTCAACG CCGACCTCAT GATGCTGAGC 
ACGCGGGACC CCCGAGACAT CGACCCCATC GTCACACGGA GGTATTATTA CTCCATCAAA 
GATGTGTCCG TGGGTGGAAT GTGCATCTGT TATGGCCACG CCCAGAATTG CCCCTTGGAC 
CCGGTCACAA AGAAACTACA CTGTGTGTGC GAACACAACA CCTGTGGAGA GAACTGTAAC 
GAGTGTTGCC CTGGTTACCA TCAGCAGCCA TGGCAACCAG GAACGGTTCG TGAGGGAAAC 
ACCTGTGAAA AATGTAACTG TCACAACAAA GCAACCGACT GTTTCTACAA TCAGACGGTG 
GCCGACCTCT CTCTGAGTTT GAATATCCAC GGCGTCCGTC GAGGGGGCGG AGTCTGTGTC 
TCCTGTCTAC ACAACACCGC CGGGCTCAAC TGTGAGAGCT GCACTGATGG CTACTACAGA 
CCTGCTGAGG TCTCTCCGTA TTCAGACTTT CCGTGTGTTG CCTGTAACTG TGACCTGAGA 
GGATCAGAGT CACCTGTGTG CTCCAGGGAC GACAAGCATC CGGGTCAGTG TGTGTGTAAG 
GAGGGTTTCG CAGGTGAACG GTGCGACACC TGTGCGTTTG GATACAGAGA CTTTCCCCAG 
TGTGTTCGCT GTGAGTGTGA CGCTGCTGGC AGCACCAACG CGGACCCCTG CAGACCCTGC 
ACCTGCAAGG TGAACGTGAT GGGAGCCCAC TGTGATCTGT GTAAACCCGG ATTCTACAAC 
CTCCAGGAGA AGAATCCGCT CGGCTGCACC GACTGTTTCT GCTTCGGCGT GTCCGACGTC 
TGTGAGAGTT CTGCTTGGTC CACAACTCAG GTGCTTCATA GAGATGCTTG GCTCCTCCCC 
CGCCCGGCTC CTCAGAACAT TACAGTCCTC CAGGACAATG ATTTACCCTC ACCTGGAAAC 
ACTTCCTCTG GCCACTCCCA CCAGGACGTT CTGCTGTGGG ATGCACCTGA CGGTTTCCTT 
GGTAACAAGC TGGGCTCCTA TGGAGGCTTC CTGAACTACT CTCTGCTGTA TGACATTCCA 
TCGGACAATG AGGACCGCTC TATTCCCGCC CACTCTGACG TCATGATCAA GGGAAACGGT 
CGTGCTCTAC ATTCCATCCC TCACTTCCTC TTTGTCTCTC CTCTTGCTGA GAGTGTGGTT 
GCCATAGTGA TGGTTGACGG GCAGTTCAGG GATGTTCAGT CAGGCGACCC CGTGAGCCGC 
GATGAGCTCC TGTCGGCCCT CGCCGACGTT ACGACTCTGA CCGTCAGGGT CGGTCTGAAC 
GCCACCGCAG ATGGACCGAT ACGTCTCAGC TGGATCTCCC TGGACGCCGC TGACCCGGGC 
CCAGGTTCTG CCCTGGACGC CGCTGACCCG GGCCCAGGTT CTGCCCAAGA GGCGGTCGCC 
GTGGAAAAGT GTGAATGTCC GTGGGGTTAC ACCGGCACTT CCTGTGAGAC GTGTCTCCCG 
GGGTTCTACA GAGTGGGCGG GGTTTTGTTC GGAGGAAACT GTATGCAGTG CGAGTGTAAC 
GACCACGCGT CAGAGTGTGA CATCAGTGGC GACTGTTGGG GTTGTACACA TAACACCAGC 
GGTCCTCACT GTGAAGAGTG TCTCCCTGGT TTCTATGGCA ACGCCACAGA GGGGACAGCA 
GATGACTGCC AGTTGTGTCC TTGTCCTCTG ACAAACCCGT CCAACAGTTT CAGTCCCACC 
TGCGTGCTGG AAGGGTCAGG AAAGGTGGTG TGTGACCAGT GCCAGGAGGG ACACACAGGA 
AGCAACTGTG AAAGGTGTGC GAGTGGTTTC TATGGTAACC CACGGGCAGC CAATGGCACC 
TGTGTGCGCT GTGATTGTCA CGGTAATGTG AACGCAAGTG AGGCGGGACA CTGTGACGTC 
ATCACCGGCG AGTGCCTGCG GTGCCTTTTC AACACCGGCG GACGCCACTG CGAGGTCTGT 
CAGCCCGGTT ACTATGGAGA CGCAGTGCTT GCCAAGAACT GTCAGGAATG TGGCTGTGAC 
GTCAACGGCG CCCCCTCCAG TGTGTGTGAC GTCACAACGG GTCAATGTCT TTGCAAGGAA 
AACGTGACAG GACGCACTTG TGACCAGTGT CAGTCGGGCT TTTTCGGCCT CCGCAGTGAG 
ATGGGCTGTC AGCTCTGTGG CTGCAACCAA TCGGGATCGC TGTCTGAGTC ATGTGACGAG 
CAGGGCCGCT GCCCGTGCGT GAAGGGTGTG GGGGGAGACA AGTGTGACCG CTGTGGTCAC 
GGCTACTACG GCGTGGACGT CAGCAGCTGC AGAGCATGCA ACTGTGTCGA CACGGGAGGG 
AACTGTGATC CCCAGAGCGG GGAGTGCATG TGCCCCGCCT TTACAGAGGG ACCCAACTGC 
AACAGATGTG AAACTGGATA CTGGGGTCAC GACCCCACAA CTGGCTGCAC GCCGTGCAGC 
TGCAGCAAGG ACGGAAGCTC CGCCCCTGAC TGTGATGCGA CCAGCGGCCA GTGTTGGTGC 
AGGAAGGGGT TTGGTGGCCG GTCATGTGAC CACTGTGCCG CTGGGCACCA TGGTTACCCG 
TCATGTTTAC CATGCGGCTG TGACCTGGCA GGAACTGATG AAAAGTTCTG CAACACGACG 
CTGAACACGT GTGACTGTCT GGACACGGGA GTGTGTGTGT GCAAGGTGGG CGTGTCTGGG 
CGCCGCTGCG AGGAGTGTGT CGCCGGCCGA TTCGCTCTGT CGGTGGACAA TCCCGATGGC 
TGCTCTCCGT GTTTCTGTTC GGGGGTGAGC GGCGACTGTG AGGAGCAGGG GGGGCTGATC 
CGACTACCGG TCACTCTGGA CCAGTCTCCT GGCCTCTTGT CAGTGGTTAG TCAGTTGAAC 
TTGCAGGGTG TCGTGTCTGG AGTTTACCAG CAGGGCGGCG ACATGCTGCT CGACACCAGG 
CACCTCAACA CCAGTGGCTG GGCTGGCCCA CTCTACTGGA GACTGCCCCC CCAGTTTGAA 
GGCCAACAGC TTCTGTCCTA CGGCGGCTCC TTATCCTACA TCATCACCTT TTATGCTGAG 
GATGGCGCTG GGCTGTCCAA TCAGGAGCCC CAGATCATGA TAAAAGGTGG GACCCTCAAG 
AAGATGGTGA TATACATGGA CGTGGTTGCC CCTAGCAACG GCATTAGGAC AAACCATGAG 
ATCAGGATGA CGGAGCACAA GTGGAAATAC TTTAACTCAG TGTTGGAGAC GTCGGTGGGT 
CGCTCGGACT TCATGTCCGT CCTGGGCAAC ATCGAGTACG TCCTCATCAA AGCGTCTTAC 
GGGAGCAGGC TGCAGCAGAG CAGGATCTCA AATATTACCA TGGAAACGGC GCTGGAGAAC 
GAGGACGGGT CAGAGGTCGG CGGGGACATG GCAAGATCGA TCGAGTCCTG CATCTGTCCC 
ACGGGATACA CCGGACTGTC CTGTCAGGAC TGTGCCGCTG GATTCTTCCG TCAGCCGCTG 
TCCGAGTTGT CACCTCAGAG TCAAAGGTTG ATGTCGGTGC GTCCGTGCGT TCCGTGTCGC 
TGCAACAACC ACAGCGAGAG CTGTGATGCA GCGAGTGGAG AATGTCAGCA GTGTCAGCAT 
CACACCAGCG GGTGGAGCTG TCAGCAGTGT TCCCAAGGGT ATCACGGGAA CGTTAGCGGC 
TCCATAAGTG ACTGCTCTCT TTGCGCCTGC CCCCTGAAGG ACAACAGCTT CAGCCCCACC 
TGTGTGTCGG AGGGAGTGCT GGGAGACTTC CGCTGCACAG CCTGTGTTCC CGGATACGAG 
GGGAGATACT GTGAAAAGTG TTCCGTCGGT TACTATGGTA ACCCCACTTT GCCAGGCGGC 
GTGTGTTCAC GGTGTAACTG TTCTGGGTGG GGTTCTCTTC AGCCGCTGTG TGACCAGCTG 
AATGGGAAGT GCGACTGTAA GGCTGGTGTC AAAGGTCAAT CATGTGACCA GTGCGAGGAC 
AGACACATCC TGCAGGAAGG AGAGTGTGTG TCGTGTGAGG ACAACTGCAC CGGTGTGATG 
CTCAACGACC TGGAAGACTT GGACGACCAC CTCGCCGCCG TCAACATCAG CAGCTTTGTT 
GTGGCCTCGT ACCTTCGGTT AGCGGCGCTG GAGAACCAGA CCAGAGGCGT CCAGGCGATG 
GCGGCGGAAA ACACTTCAGC CTCTCTGCAG CTGAGCGGCC TCGAAGAGCA ACTCGGCCTC 
ATGAGCCTCG ACCTCGGCGT CTTGCTGCAG CTGGTGGTAA ATATGTCTGG TGACCTTCAG 
AGACTGGACA CATCTGCTCA GGACACCATC CATCAGGGCT CACAGCAAAT CCAGAACATC 
AACATCCTTC AAGACAAAGT GCAGGCTCTT CAGCAGGAGG CCGAACGCCT GAATGCGACT 
GCAGAAGAAG AGCTGACGAC AGCCAACAGA ACACATCTGC TGCAAGAACT TGAGTTGATG 
TTGAGAAGCA TGAGAGTGAC CAACCTCACC GCTGCCAGCA GCCTGGCCAA TCAGGAGCTC 
CTACGCGCCA CATCTGTCCT CTCCCTGCAG TCGGACCTGC TCTCCCGCAG GGCCGCAGTG 
GACGACGGCC TCCGCCACCT TTCGACCTCC CTCAGCAGTA ACAGCGGAAC ACTGCGCCAC 
CTACAGGCAC AACTGAGCGG CGCCACACAA CAAAACATGC AGACACACGT GCTTCTGGAG 
GTCCTGCACA ACCTGACGCA GCAGCACCAG TCTGTCCAGC AGGACATCAG CCAGGAGCGC 
CTCCTGGTGG CGGGACTGAT GCAGGACACT CAGCTCGTGC TGGGCGACAG CTTCAGCATC 
ATGGAGGACC TAACCAACAC CACCTCGGTG GAGGTGTTTG GCAGTTACCT GGATCAGTGG 
AGCCCCCTGC TGAGGAAACG GGTGGATGGT TTGGTTGGTG GTCTGAAGAT GACGGATTCC 
CTGGAGAAGG TCTACCTTTC AGAGAAACAC GCCCAGCAGC TGGACAACGA CGCCCACTCC 
CTGCACAGCT CGCTGCTGTC TGTGTATAAT GCGTCTCAAA ATGGCAGCCA GCTGCTTCAG 
GGGGGGAACA GCGGCGTCCC GGAGCAGGTG GAGGCGCTGC GGCAGGAGGC CCTCATGGCC 
GACCGGTCCG CCCGGCTGCT GTTCAACGCG ACGGTCCAAT CAGAACCGCC CCTGCCAGAG 
GAGAGCAGGA CTCGCCTGAA CATCAGCGCC GCTGTTTTGG AAACCTGTCG ACAGATCGGC 
GCTTCATCCG CAGACTTGCA GATGAACGTC TCCATGGTAA CCAGCAGACT AGGATTATTT 
AAAGAAAGCT TCCAGAAGTT CGGTCTCCTA TTTCATCATT CGATCACAGA GCTGCAGAGC 
CTCTCAAATG GTTCACTGAC CGAGCTGCAA CAAGTTCAGC TGCAGGCTGC AGAAGCAGAC 
TCCAGGCTAC AGGGGGCGCT ACTGAGGCTA CAGAAACTCA GACAGCAGCT CCAGGATTCT 
TCCTCTGCAG TGGAAAACAC CAGCAACACC GTCACAGAGA CCCAGCAGCT CACAGCCCTC 
ACACACTCCA CAGTCGATCA GGCGCGTCAG AAACTGGGCG AGGTGGAGCG TCGCACAGAG 
CGCCTTATCG ACCAGATCAA GCCCCTGAGC ATGCTGGGAG AAACGATAAA CAGGAACCTG 
TCTGACATCA GAGAGCTGAT CAATCAGGCC CGAAGACAGG CCGCCTCGAT TAAGGTGGCG 
GTGCAGGCAG ACAGAAGCTG CGTGCGCTCC TACAGGCCAC AGTCCGAGTC CAGGAACTTC 
AACACGCTGA GTCTTGTCTT GAGGACGTCC AGTCCAAACA ACCTGCTGTT CTTCCAGGGC 
AGCAACACCA CGGTGGACTT CCTGGCTCTG GAGATGTATC GTGGGAAGGT GTCTCTGCTG 
TGGGATCTGG GTTCAGGTAG CACCAGACTG GAGTTTCCCG GACTGGACAT CACCAACAAC 
AGATGGACCA GAATCAACGC CACGCGGTTT GGGGCTCATG CGTCTCTGTG GGTTCATCAG 
CTGGAGTCGG ACTCGGAGCC CCTCCCTGCA GTAACGGCCA CCTCCCCGGG TCCCGCCAGG 
ATTTTAGACA CGGACAGAAA CTCTTGGCTC TACATTGGTG GACTGGGAGC TCACGGACAG 
AGGCCGGCAG CCCTGCGCTC CACCACATTC CAGGGATGTC TGGGCGAAGC GGCCTTGAAC 
GAGCGGAACA TCGGCCTCTG GAACTACCAC AGAAGGGAGG GAGAGTGTGG AGGCTGCTTC 
AGCAGTCCAC AAACGGAGGA GACCTCCTTC CATTTCGACG GATCCGGATT CTCTTTGGTC 
CAGAAGCCTC TACGGGCGAC CTCCACCTCC ATCGTCTTGC TGTTCAAAAC ACTGTCACCA 
GGGGGGTTAC TGTTGTACCT GGCCTCCAAC AACACGAGGG ACTTTCTGTC CATCGAGCTA 
GTAGAGGGGC AGGTTCGCCT GACCTTTGAC CTCGGCTCAG GTGCACTCGT CCTGACCTCC 
AGCAGGAAGT ACAACACTGG CGTCTGGTAC AAGATCACGC TGCAGAGGAA CAAACGTAAA 
GGTTACCTTT CCATCATGGC TGCTGACCAG TCGTCGGAAA AGGAGGTGTT GGAGGCGGAG 
TCTCCCGGGC TGGCGTCCGA CCTTAACCGC TCCGACTTTG ACCCCATCTA CATCGGAGGA 
CTTCCTGCCT CCAGACCCAT CAGGCGACAG GTGGTGTCCA GGGCTTACGT GGGCTGTATA 
AAAAATGTGG AGATCGCCCG GTCCAACTTT GACCTGCTGA GAGACGCTTT TGGCGTCAGG 
AAGGGCTGCG TTCTTGAGGC CCTCAGGAGC GTGTCGCTGT TGACCGGCGG CTTCGTTCAA 
ATGGCTCCTC GAGCTCTTGG ACCAGAGGTG GAGCTTCTCT TTTCCTTCTC CACCAACAAC 
CGGTCAGGAA TTCTGCTCGC TGCCGTCACC GACGACCACG TTCAGAGGCG GGTAGGGCGG 
AAAGTCCGCT TCCTGTCCAT CCACCTGGCC TCAGGTGCGG TGGAGGCCGA CATTGGTGAA 
GTGGGAGGAG CCACACAAAC CGTGACAGTG GTGAAACCTA AGGGAGGATT TTTTAGCGAT 
GGTCTGAAAC ACTCTGTCAT CGTTACCGTC AACAAGAAGA CCCTGTCCAT ACAGGTGGAT 
GAGACACACC TGAAGTCCAT CCCTCTGTTG CCGGGCAGAT TTTCTAATTT GTCTCCAGCC 
TCATTTTTTA TTGGTGGAGT ACCTGTAGGG GCGGGGCTCC AGCTGCCCTC CAGGCTGCGG 
CAGATTTCTT TATCCTTCAG AGGCTGCATC CAACACCTGG TGTTAGGAGG AGGAGCGCTC 
GTTGACCTCT CAGGAGCGTT GAGGTATGAG GGGGTGACCC TGGACAGCTG CCTGCTGGAA 
GAGGCGGCCA GGGGGGCGGT GCTTCCTGAT GACCCGGACA CAGAACCAAC CATGGACCCG 
GTCCGCCTCC CTGTGGTACC GCCCACCCCG CTGGGTGTCC TGCCCCCAGG CGCTCTAACG 
TGTGCAGCAC AGGTGGACTC CTCCTTCCTG CCCTCCGCAG CTCAGTTCGG TTTGTCCAAA 
CACAGTCACA TGACGTTCCT GATCAACCCC GGCGCCCTCA GGAAAAGTGT GTCTCTGAAG 
ATATCGGCTC GGACCCGATC TCCGGACGGC CTGCTCCTCC TCCTCTCCGA CGCCAAACAG 
ATGGACTTCG CCGTCGTCAA ACTAGCGGGG GGGAAGGTGA CGTTGTCAGC CGACCTGGGG 
AAGGGTCCCA CCTCCGTCAC CTCACCTGTG TCCATCACTG ACGGACTCTG GCACACTGTG 
AGCGCCGAGG TCAGCCGCCG CTCGCTCGTC CTGGCGGTCG ACGGTTCAAA GCCCGGCTCG 
GCGTCCATCA GGGGGAACCA GCTGGACGTG GACAGGCGCC TGTACCTGGG GGGGCTGCCG 
CACGCACACG CCACCAGGAG GATTAACGTC AGCAGCAGCT TCCAGGGCTG CGTTCGGTCC 
GTCCACCTGA ACGGCGCCGT GTTGGATCTG ACCAAACCGG CCTCGCGCCA CAACGTCACT 
TCCTGTTTCA CCGATGACCA GGCAGGAAGC TACTTCGACG GCAGCGGACA CGCCCTCTTC 
ATGCGCGATG GCTATAAAGT CGGATCCGAT GTTTCTGTAT CTCTGGAGTT CCGGACCAGC 
CAATCAGAAG GAGTGTTTTT AGGAATTAGC AGTGCCAAAA TTGATGCCAT CGGACTCGAG 
ATGATCAAAG GACAGGTGGT GTTTAACGTG AACAATGGCG CCGGGAGAGT GACGGTGCGC 
TCCGTCGGTC CGATGCTGTG TGACGGCCGA TGGCACCGCG TCCTGGCCAG GAAAACCAAA 
CACAGCCTGA ATTTACAGGT GGATGGGCGG AGCTACATCA CCCCCAACCC TTACCCACAA 
TCCACCTCGG CTGAGACGAA AAACCCAGTT TACCTGGGAG GCTACCCTGT CGGGGTAAAG 
CAGAACTGCC TGTCAATCAC CACCCGGTTC AGAGGTTGTC TGAAGAAGAT ACAGCTCATT 
AAACCGCACC TGAGCGATAC GCTGGACCTA AGCTCCGCCC ACGTCCTCTC AGGTGTCACT 
CCTGACTCCT GCCCTGTAGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029698574.1
CDS	90..9353
Translation	MKLLLLVLLLDGAAAQLRGLFPAILNLATNAVVDSNATCGDPDPEVYCKLVEHVPGRPVKNPHCARCDAASLLLRERHPITNAIDGTNRWWQSPSIKNGRQFHWVTITLDLKQIFQVAYVIIKAANSPRPGNWILERSLDGVTFDPWQYYAISNSECLSHYNVTPRLGPPTYKRDTEVICTSYYSRLNPLENGEIHTSLINGRPGADDLTPELLDFTSARYIRLRLQRIRTLNADLMMLSTRDPRDIDPIVTRRYYYSIKDVSVGGMCICYGHAQNCPLDPVTKKLHCVCEHNTCGENCNECCPGYHQQPWQPGTVREGNTCEKCNCHNKATDCFYNQTVADLSLSLNIHGVRRGGGVCVSCLHNTAGLNCESCTDGYYRPAEVSPYSDFPCVACNCDLRGSESPVCSRDDKHPGQCVCKEGFAGERCDTCAFGYRDFPQCVRCECDAAGSTNADPCRPCTCKVNVMGAHCDLCKPGFYNLQEKNPLGCTDCFCFGVSDVCESSAWSTTQVLHRDAWLLPRPAPQNITVLQDNDLPSPGNTSSGHSHQDVLLWDAPDGFLGNKLGSYGGFLNYSLLYDIPSDNEDRSIPAHSDVMIKGNGRALHSIPHFLFVSPLAESVVAIVMVDGQFRDVQSGDPVSRDELLSALADVTTLTVRVGLNATADGPIRLSWISLDAADPGPGSALDAADPGPGSAQEAVAVEKCECPWGYTGTSCETCLPGFYRVGGVLFGGNCMQCECNDHASECDISGDCWGCTHNTSGPHCEECLPGFYGNATEGTADDCQLCPCPLTNPSNSFSPTCVLEGSGKVVCDQCQEGHTGSNCERCASGFYGNPRAANGTCVRCDCHGNVNASEAGHCDVITGECLRCLFNTGGRHCEVCQPGYYGDAVLAKNCQECGCDVNGAPSSVCDVTTGQCLCKENVTGRTCDQCQSGFFGLRSEMGCQLCGCNQSGSLSESCDEQGRCPCVKGVGGDKCDRCGHGYYGVDVSSCRACNCVDTGGNCDPQSGECMCPAFTEGPNCNRCETGYWGHDPTTGCTPCSCSKDGSSAPDCDATSGQCWCRKGFGGRSCDHCAAGHHGYPSCLPCGCDLAGTDEKFCNTTLNTCDCLDTGVCVCKVGVSGRRCEECVAGRFALSVDNPDGCSPCFCSGVSGDCEEQGGLIRLPVTLDQSPGLLSVVSQLNLQGVVSGVYQQGGDMLLDTRHLNTSGWAGPLYWRLPPQFEGQQLLSYGGSLSYIITFYAEDGAGLSNQEPQIMIKGGTLKKMVIYMDVVAPSNGIRTNHEIRMTEHKWKYFNSVLETSVGRSDFMSVLGNIEYVLIKASYGSRLQQSRISNITMETALENEDGSEVGGDMARSIESCICPTGYTGLSCQDCAAGFFRQPLSELSPQSQRLMSVRPCVPCRCNNHSESCDAASGECQQCQHHTSGWSCQQCSQGYHGNVSGSISDCSLCACPLKDNSFSPTCVSEGVLGDFRCTACVPGYEGRYCEKCSVGYYGNPTLPGGVCSRCNCSGWGSLQPLCDQLNGKCDCKAGVKGQSCDQCEDRHILQEGECVSCEDNCTGVMLNDLEDLDDHLAAVNISSFVVASYLRLAALENQTRGVQAMAAENTSASLQLSGLEEQLGLMSLDLGVLLQLVVNMSGDLQRLDTSAQDTIHQGSQQIQNINILQDKVQALQQEAERLNATAEEELTTANRTHLLQELELMLRSMRVTNLTAASSLANQELLRATSVLSLQSDLLSRRAAVDDGLRHLSTSLSSNSGTLRHLQAQLSGATQQNMQTHVLLEVLHNLTQQHQSVQQDISQERLLVAGLMQDTQLVLGDSFSIMEDLTNTTSVEVFGSYLDQWSPLLRKRVDGLVGGLKMTDSLEKVYLSEKHAQQLDNDAHSLHSSLLSVYNASQNGSQLLQGGNSGVPEQVEALRQEALMADRSARLLFNATVQSEPPLPEESRTRLNISAAVLETCRQIGASSADLQMNVSMVTSRLGLFKESFQKFGLLFHHSITELQSLSNGSLTELQQVQLQAAEADSRLQGALLRLQKLRQQLQDSSSAVENTSNTVTETQQLTALTHSTVDQARQKLGEVERRTERLIDQIKPLSMLGETINRNLSDIRELINQARRQAASIKVAVQADRSCVRSYRPQSESRNFNTLSLVLRTSSPNNLLFFQGSNTTVDFLALEMYRGKVSLLWDLGSGSTRLEFPGLDITNNRWTRINATRFGAHASLWVHQLESDSEPLPAVTATSPGPARILDTDRNSWLYIGGLGAHGQRPAALRSTTFQGCLGEAALNERNIGLWNYHRREGECGGCFSSPQTEETSFHFDGSGFSLVQKPLRATSTSIVLLFKTLSPGGLLLYLASNNTRDFLSIELVEGQVRLTFDLGSGALVLTSSRKYNTGVWYKITLQRNKRKGYLSIMAADQSSEKEVLEAESPGLASDLNRSDFDPIYIGGLPASRPIRRQVVSRAYVGCIKNVEIARSNFDLLRDAFGVRKGCVLEALRSVSLLTGGFVQMAPRALGPEVELLFSFSTNNRSGILLAAVTDDHVQRRVGRKVRFLSIHLASGAVEADIGEVGGATQTVTVVKPKGGFFSDGLKHSVIVTVNKKTLSIQVDETHLKSIPLLPGRFSNLSPASFFIGGVPVGAGLQLPSRLRQISLSFRGCIQHLVLGGGALVDLSGALRYEGVTLDSCLLEEAARGAVLPDDPDTEPTMDPVRLPVVPPTPLGVLPPGALTCAAQVDSSFLPSAAQFGLSKHSHMTFLINPGALRKSVSLKISARTRSPDGLLLLLSDAKQMDFAVVKLAGGKVTLSADLGKGPTSVTSPVSITDGLWHTVSAEVSRRSLVLAVDGSKPGSASIRGNQLDVDRRLYLGGLPHAHATRRINVSSSFQGCVRSVHLNGAVLDLTKPASRHNVTSCFTDDQAGSYFDGSGHALFMRDGYKVGSDVSVSLEFRTSQSEGVFLGISSAKIDAIGLEMIKGQVVFNVNNGAGRVTVRSVGPMLCDGRWHRVLARKTKHSLNLQVDGRSYITPNPYPQSTSAETKNPVYLGGYPVGVKQNCLSITTRFRGCLKKIQLIKPHLSDTLDLSSAHVLSGVTPDSCPVG

Target ORF information:

RefSeq Version	XM_029842714.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029842714.1

ORF Insert Sequence:

ATGAAGTTGC TGCTGTTGGT GCTGCTGCTG GATGGAGCTG CAGCTCAGCT GAGAGGCCTG 
TTTCCTGCCA TCTTGAACTT GGCCACCAAC GCCGTGGTCG ACAGCAACGC CACGTGCGGC 
GACCCGGACC CGGAGGTCTA CTGTAAACTG GTGGAGCACG TTCCGGGTCG ACCCGTAAAG 
AACCCTCACT GCGCACGCTG TGACGCCGCC TCGCTTCTGC TGAGAGAACG ACATCCCATC 
ACCAACGCCA TCGACGGAAC CAACCGCTGG TGGCAGAGTC CCAGTATCAA GAATGGGCGG 
CAGTTTCACT GGGTCACCAT CACGCTGGAC CTGAAACAGA TCTTCCAGGT CGCCTACGTC 
ATCATCAAAG CAGCGAACTC TCCTCGCCCA GGTAACTGGA TCCTGGAGCG CTCCCTGGAC 
GGGGTGACCT TTGACCCCTG GCAGTATTAC GCAATTAGCA ACTCCGAGTG CTTGTCCCAT 
TACAACGTCA CCCCGAGACT CGGACCTCCG ACCTACAAGA GGGACACGGA GGTCATCTGC 
ACGTCCTACT ACTCCAGACT GAACCCTCTG GAGAACGGAG AGATCCACAC CTCGCTGATC 
AACGGCCGCC CCGGAGCGGA CGACCTGACG CCCGAGCTCT TGGACTTCAC CTCGGCCCGT 
TACATCCGAC TCAGGCTCCA GCGGATCAGG ACGCTCAACG CCGACCTCAT GATGCTGAGC 
ACGCGGGACC CCCGAGACAT CGACCCCATC GTCACACGGA GGTATTATTA CTCCATCAAA 
GATGTGTCCG TGGGTGGAAT GTGCATCTGT TATGGCCACG CCCAGAATTG CCCCTTGGAC 
CCGGTCACAA AGAAACTACA CTGTGTGTGC GAACACAACA CCTGTGGAGA GAACTGTAAC 
GAGTGTTGCC CTGGTTACCA TCAGCAGCCA TGGCAACCAG GAACGGTTCG TGAGGGAAAC 
ACCTGTGAAA AATGTAACTG TCACAACAAA GCAACCGACT GTTTCTACAA TCAGACGGTG 
GCCGACCTCT CTCTGAGTTT GAATATCCAC GGCGTCCGTC GAGGGGGCGG AGTCTGTGTC 
TCCTGTCTAC ACAACACCGC CGGGCTCAAC TGTGAGAGCT GCACTGATGG CTACTACAGA 
CCTGCTGAGG TCTCTCCGTA TTCAGACTTT CCGTGTGTTG CCTGTAACTG TGACCTGAGA 
GGATCAGAGT CACCTGTGTG CTCCAGGGAC GACAAGCATC CGGGTCAGTG TGTGTGTAAG 
GAGGGTTTCG CAGGTGAACG GTGCGACACC TGTGCGTTTG GATACAGAGA CTTTCCCCAG 
TGTGTTCGCT GTGAGTGTGA CGCTGCTGGC AGCACCAACG CGGACCCCTG CAGACCCTGC 
ACCTGCAAGG TGAACGTGAT GGGAGCCCAC TGTGATCTGT GTAAACCCGG ATTCTACAAC 
CTCCAGGAGA AGAATCCGCT CGGCTGCACC GACTGTTTCT GCTTCGGCGT GTCCGACGTC 
TGTGAGAGTT CTGCTTGGTC CACAACTCAG GTGCTTCATA GAGATGCTTG GCTCCTCCCC 
CGCCCGGCTC CTCAGAACAT TACAGTCCTC CAGGACAATG ATTTACCCTC ACCTGGAAAC 
ACTTCCTCTG GCCACTCCCA CCAGGACGTT CTGCTGTGGG ATGCACCTGA CGGTTTCCTT 
GGTAACAAGC TGGGCTCCTA TGGAGGCTTC CTGAACTACT CTCTGCTGTA TGACATTCCA 
TCGGACAATG AGGACCGCTC TATTCCCGCC CACTCTGACG TCATGATCAA GGGAAACGGT 
CGTGCTCTAC ATTCCATCCC TCACTTCCTC TTTGTCTCTC CTCTTGCTGA GAGTGTGGTT 
GCCATAGTGA TGGTTGACGG GCAGTTCAGG GATGTTCAGT CAGGCGACCC CGTGAGCCGC 
GATGAGCTCC TGTCGGCCCT CGCCGACGTT ACGACTCTGA CCGTCAGGGT CGGTCTGAAC 
GCCACCGCAG ATGGACCGAT ACGTCTCAGC TGGATCTCCC TGGACGCCGC TGACCCGGGC 
CCAGGTTCTG CCCTGGACGC CGCTGACCCG GGCCCAGGTT CTGCCCAAGA GGCGGTCGCC 
GTGGAAAAGT GTGAATGTCC GTGGGGTTAC ACCGGCACTT CCTGTGAGAC GTGTCTCCCG 
GGGTTCTACA GAGTGGGCGG GGTTTTGTTC GGAGGAAACT GTATGCAGTG CGAGTGTAAC 
GACCACGCGT CAGAGTGTGA CATCAGTGGC GACTGTTGGG GTTGTACACA TAACACCAGC 
GGTCCTCACT GTGAAGAGTG TCTCCCTGGT TTCTATGGCA ACGCCACAGA GGGGACAGCA 
GATGACTGCC AGTTGTGTCC TTGTCCTCTG ACAAACCCGT CCAACAGTTT CAGTCCCACC 
TGCGTGCTGG AAGGGTCAGG AAAGGTGGTG TGTGACCAGT GCCAGGAGGG ACACACAGGA 
AGCAACTGTG AAAGGTGTGC GAGTGGTTTC TATGGTAACC CACGGGCAGC CAATGGCACC 
TGTGTGCGCT GTGATTGTCA CGGTAATGTG AACGCAAGTG AGGCGGGACA CTGTGACGTC 
ATCACCGGCG AGTGCCTGCG GTGCCTTTTC AACACCGGCG GACGCCACTG CGAGGTCTGT 
CAGCCCGGTT ACTATGGAGA CGCAGTGCTT GCCAAGAACT GTCAGGAATG TGGCTGTGAC 
GTCAACGGCG CCCCCTCCAG TGTGTGTGAC GTCACAACGG GTCAATGTCT TTGCAAGGAA 
AACGTGACAG GACGCACTTG TGACCAGTGT CAGTCGGGCT TTTTCGGCCT CCGCAGTGAG 
ATGGGCTGTC AGCTCTGTGG CTGCAACCAA TCGGGATCGC TGTCTGAGTC ATGTGACGAG 
CAGGGCCGCT GCCCGTGCGT GAAGGGTGTG GGGGGAGACA AGTGTGACCG CTGTGGTCAC 
GGCTACTACG GCGTGGACGT CAGCAGCTGC AGAGCATGCA ACTGTGTCGA CACGGGAGGG 
AACTGTGATC CCCAGAGCGG GGAGTGCATG TGCCCCGCCT TTACAGAGGG ACCCAACTGC 
AACAGATGTG AAACTGGATA CTGGGGTCAC GACCCCACAA CTGGCTGCAC GCCGTGCAGC 
TGCAGCAAGG ACGGAAGCTC CGCCCCTGAC TGTGATGCGA CCAGCGGCCA GTGTTGGTGC 
AGGAAGGGGT TTGGTGGCCG GTCATGTGAC CACTGTGCCG CTGGGCACCA TGGTTACCCG 
TCATGTTTAC CATGCGGCTG TGACCTGGCA GGAACTGATG AAAAGTTCTG CAACACGACG 
CTGAACACGT GTGACTGTCT GGACACGGGA GTGTGTGTGT GCAAGGTGGG CGTGTCTGGG 
CGCCGCTGCG AGGAGTGTGT CGCCGGCCGA TTCGCTCTGT CGGTGGACAA TCCCGATGGC 
TGCTCTCCGT GTTTCTGTTC GGGGGTGAGC GGCGACTGTG AGGAGCAGGG GGGGCTGATC 
CGACTACCGG TCACTCTGGA CCAGTCTCCT GGCCTCTTGT CAGTGGTTAG TCAGTTGAAC 
TTGCAGGGTG TCGTGTCTGG AGTTTACCAG CAGGGCGGCG ACATGCTGCT CGACACCAGG 
CACCTCAACA CCAGTGGCTG GGCTGGCCCA CTCTACTGGA GACTGCCCCC CCAGTTTGAA 
GGCCAACAGC TTCTGTCCTA CGGCGGCTCC TTATCCTACA TCATCACCTT TTATGCTGAG 
GATGGCGCTG GGCTGTCCAA TCAGGAGCCC CAGATCATGA TAAAAGGTGG GACCCTCAAG 
AAGATGGTGA TATACATGGA CGTGGTTGCC CCTAGCAACG GCATTAGGAC AAACCATGAG 
ATCAGGATGA CGGAGCACAA GTGGAAATAC TTTAACTCAG TGTTGGAGAC GTCGGTGGGT 
CGCTCGGACT TCATGTCCGT CCTGGGCAAC ATCGAGTACG TCCTCATCAA AGCGTCTTAC 
GGGAGCAGGC TGCAGCAGAG CAGGATCTCA AATATTACCA TGGAAACGGC GCTGGAGAAC 
GAGGACGGGT CAGAGGTCGG CGGGGACATG GCAAGATCGA TCGAGTCCTG CATCTGTCCC 
ACGGGATACA CCGGACTGTC CTGTCAGGAC TGTGCCGCTG GATTCTTCCG TCAGCCGCTG 
TCCGAGTTGT CACCTCAGAG TCAAAGGTTG ATGTCGGTGC GTCCGTGCGT TCCGTGTCGC 
TGCAACAACC ACAGCGAGAG CTGTGATGCA GCGAGTGGAG AATGTCAGCA GTGTCAGCAT 
CACACCAGCG GGTGGAGCTG TCAGCAGTGT TCCCAAGGGT ATCACGGGAA CGTTAGCGGC 
TCCATAAGTG ACTGCTCTCT TTGCGCCTGC CCCCTGAAGG ACAACAGCTT CAGCCCCACC 
TGTGTGTCGG AGGGAGTGCT GGGAGACTTC CGCTGCACAG CCTGTGTTCC CGGATACGAG 
GGGAGATACT GTGAAAAGTG TTCCGTCGGT TACTATGGTA ACCCCACTTT GCCAGGCGGC 
GTGTGTTCAC GGTGTAACTG TTCTGGGTGG GGTTCTCTTC AGCCGCTGTG TGACCAGCTG 
AATGGGAAGT GCGACTGTAA GGCTGGTGTC AAAGGTCAAT CATGTGACCA GTGCGAGGAC 
AGACACATCC TGCAGGAAGG AGAGTGTGTG TCGTGTGAGG ACAACTGCAC CGGTGTGATG 
CTCAACGACC TGGAAGACTT GGACGACCAC CTCGCCGCCG TCAACATCAG CAGCTTTGTT 
GTGGCCTCGT ACCTTCGGTT AGCGGCGCTG GAGAACCAGA CCAGAGGCGT CCAGGCGATG 
GCGGCGGAAA ACACTTCAGC CTCTCTGCAG CTGAGCGGCC TCGAAGAGCA ACTCGGCCTC 
ATGAGCCTCG ACCTCGGCGT CTTGCTGCAG CTGGTGGTAA ATATGTCTGG TGACCTTCAG 
AGACTGGACA CATCTGCTCA GGACACCATC CATCAGGGCT CACAGCAAAT CCAGAACATC 
AACATCCTTC AAGACAAAGT GCAGGCTCTT CAGCAGGAGG CCGAACGCCT GAATGCGACT 
GCAGAAGAAG AGCTGACGAC AGCCAACAGA ACACATCTGC TGCAAGAACT TGAGTTGATG 
TTGAGAAGCA TGAGAGTGAC CAACCTCACC GCTGCCAGCA GCCTGGCCAA TCAGGAGCTC 
CTACGCGCCA CATCTGTCCT CTCCCTGCAG TCGGACCTGC TCTCCCGCAG GGCCGCAGTG 
GACGACGGCC TCCGCCACCT TTCGACCTCC CTCAGCAGTA ACAGCGGAAC ACTGCGCCAC 
CTACAGGCAC AACTGAGCGG CGCCACACAA CAAAACATGC AGACACACGT GCTTCTGGAG 
GTCCTGCACA ACCTGACGCA GCAGCACCAG TCTGTCCAGC AGGACATCAG CCAGGAGCGC 
CTCCTGGTGG CGGGACTGAT GCAGGACACT CAGCTCGTGC TGGGCGACAG CTTCAGCATC 
ATGGAGGACC TAACCAACAC CACCTCGGTG GAGGTGTTTG GCAGTTACCT GGATCAGTGG 
AGCCCCCTGC TGAGGAAACG GGTGGATGGT TTGGTTGGTG GTCTGAAGAT GACGGATTCC 
CTGGAGAAGG TCTACCTTTC AGAGAAACAC GCCCAGCAGC TGGACAACGA CGCCCACTCC 
CTGCACAGCT CGCTGCTGTC TGTGTATAAT GCGTCTCAAA ATGGCAGCCA GCTGCTTCAG 
GGGGGGAACA GCGGCGTCCC GGAGCAGGTG GAGGCGCTGC GGCAGGAGGC CCTCATGGCC 
GACCGGTCCG CCCGGCTGCT GTTCAACGCG ACGGTCCAAT CAGAACCGCC CCTGCCAGAG 
GAGAGCAGGA CTCGCCTGAA CATCAGCGCC GCTGTTTTGG AAACCTGTCG ACAGATCGGC 
GCTTCATCCG CAGACTTGCA GATGAACGTC TCCATGGTAA CCAGCAGACT AGGATTATTT 
AAAGAAAGCT TCCAGAAGTT CGGTCTCCTA TTTCATCATT CGATCACAGA GCTGCAGAGC 
CTCTCAAATG GTTCACTGAC CGAGCTGCAA CAAGTTCAGC TGCAGGCTGC AGAAGCAGAC 
TCCAGGCTAC AGGGGGCGCT ACTGAGGCTA CAGAAACTCA GACAGCAGCT CCAGGATTCT 
TCCTCTGCAG TGGAAAACAC CAGCAACACC GTCACAGAGA CCCAGCAGCT CACAGCCCTC 
ACACACTCCA CAGTCGATCA GGCGCGTCAG AAACTGGGCG AGGTGGAGCG TCGCACAGAG 
CGCCTTATCG ACCAGATCAA GCCCCTGAGC ATGCTGGGAG AAACGATAAA CAGGAACCTG 
TCTGACATCA GAGAGCTGAT CAATCAGGCC CGAAGACAGG CCGCCTCGAT TAAGGTGGCG 
GTGCAGGCAG ACAGAAGCTG CGTGCGCTCC TACAGGCCAC AGTCCGAGTC CAGGAACTTC 
AACACGCTGA GTCTTGTCTT GAGGACGTCC AGTCCAAACA ACCTGCTGTT CTTCCAGGGC 
AGCAACACCA CGGTGGACTT CCTGGCTCTG GAGATGTATC GTGGGAAGGT GTCTCTGCTG 
TGGGATCTGG GTTCAGGTAG CACCAGACTG GAGTTTCCCG GACTGGACAT CACCAACAAC 
AGATGGACCA GAATCAACGC CACGCGGTTT GGGGCTCATG CGTCTCTGTG GGTTCATCAG 
CTGGAGTCGG ACTCGGAGCC CCTCCCTGCA GTAACGGCCA CCTCCCCGGG TCCCGCCAGG 
ATTTTAGACA CGGACAGAAA CTCTTGGCTC TACATTGGTG GACTGGGAGC TCACGGACAG 
AGGCCGGCAG CCCTGCGCTC CACCACATTC CAGGGATGTC TGGGCGAAGC GGCCTTGAAC 
GAGCGGAACA TCGGCCTCTG GAACTACCAC AGAAGGGAGG GAGAGTGTGG AGGCTGCTTC 
AGCAGTCCAC AAACGGAGGA GACCTCCTTC CATTTCGACG GATCCGGATT CTCTTTGGTC 
CAGAAGCCTC TACGGGCGAC CTCCACCTCC ATCGTCTTGC TGTTCAAAAC ACTGTCACCA 
GGGGGGTTAC TGTTGTACCT GGCCTCCAAC AACACGAGGG ACTTTCTGTC CATCGAGCTA 
GTAGAGGGGC AGGTTCGCCT GACCTTTGAC CTCGGCTCAG GTGCACTCGT CCTGACCTCC 
AGCAGGAAGT ACAACACTGG CGTCTGGTAC AAGATCACGC TGCAGAGGAA CAAACGTAAA 
GGTTACCTTT CCATCATGGC TGCTGACCAG TCGTCGGAAA AGGAGGTGTT GGAGGCGGAG 
TCTCCCGGGC TGGCGTCCGA CCTTAACCGC TCCGACTTTG ACCCCATCTA CATCGGAGGA 
CTTCCTGCCT CCAGACCCAT CAGGCGACAG GTGGTGTCCA GGGCTTACGT GGGCTGTATA 
AAAAATGTGG AGATCGCCCG GTCCAACTTT GACCTGCTGA GAGACGCTTT TGGCGTCAGG 
AAGGGCTGCG TTCTTGAGGC CCTCAGGAGC GTGTCGCTGT TGACCGGCGG CTTCGTTCAA 
ATGGCTCCTC GAGCTCTTGG ACCAGAGGTG GAGCTTCTCT TTTCCTTCTC CACCAACAAC 
CGGTCAGGAA TTCTGCTCGC TGCCGTCACC GACGACCACG TTCAGAGGCG GGTAGGGCGG 
AAAGTCCGCT TCCTGTCCAT CCACCTGGCC TCAGGTGCGG TGGAGGCCGA CATTGGTGAA 
GTGGGAGGAG CCACACAAAC CGTGACAGTG GTGAAACCTA AGGGAGGATT TTTTAGCGAT 
GGTCTGAAAC ACTCTGTCAT CGTTACCGTC AACAAGAAGA CCCTGTCCAT ACAGGTGGAT 
GAGACACACC TGAAGTCCAT CCCTCTGTTG CCGGGCAGAT TTTCTAATTT GTCTCCAGCC 
TCATTTTTTA TTGGTGGAGT ACCTGTAGGG GCGGGGCTCC AGCTGCCCTC CAGGCTGCGG 
CAGATTTCTT TATCCTTCAG AGGCTGCATC CAACACCTGG TGTTAGGAGG AGGAGCGCTC 
GTTGACCTCT CAGGAGCGTT GAGGTATGAG GGGGTGACCC TGGACAGCTG CCTGCTGGAA 
GAGGCGGCCA GGGGGGCGGT GCTTCCTGAT GACCCGGACA CAGAACCAAC CATGGACCCG 
GTCCGCCTCC CTGTGGTACC GCCCACCCCG CTGGGTGTCC TGCCCCCAGG CGCTCTAACG 
TGTGCAGCAC AGGTGGACTC CTCCTTCCTG CCCTCCGCAG CTCAGTTCGG TTTGTCCAAA 
CACAGTCACA TGACGTTCCT GATCAACCCC GGCGCCCTCA GGAAAAGTGT GTCTCTGAAG 
ATATCGGCTC GGACCCGATC TCCGGACGGC CTGCTCCTCC TCCTCTCCGA CGCCAAACAG 
ATGGACTTCG CCGTCGTCAA ACTAGCGGGG GGGAAGGTGA CGTTGTCAGC CGACCTGGGG 
AAGGGTCCCA CCTCCGTCAC CTCACCTGTG TCCATCACTG ACGGACTCTG GCACACTGTG 
AGCGCCGAGG TCAGCCGCCG CTCGCTCGTC CTGGCGGTCG ACGGTTCAAA GCCCGGCTCG 
GCGTCCATCA GGGGGAACCA GCTGGACGTG GACAGGCGCC TGTACCTGGG GGGGCTGCCG 
CACGCACACG CCACCAGGAG GATTAACGTC AGCAGCAGCT TCCAGGGCTG CGTTCGGTCC 
GTCCACCTGA ACGGCGCCGT GTTGGATCTG ACCAAACCGG CCTCGCGCCA CAACGTCACT 
TCCTGTTTCA CCGATGACCA GGCAGGAAGC TACTTCGACG GCAGCGGACA CGCCCTCTTC 
ATGCGCGATG GCTATAAAGT CGGATCCGAT GTTTCTGTAT CTCTGGAGTT CCGGACCAGC 
CAATCAGAAG GAGTGTTTTT AGGAATTAGC AGTGCCAAAA TTGATGCCAT CGGACTCGAG 
ATGATCAAAG GACAGGTGGT GTTTAACGTG AACAATGGCG CCGGGAGAGT GACGGTGCGC 
TCCGTCGGTC CGATGCTGTG TGACGGCCGA TGGCACCGCG TCCTGGCCAG GAAAACCAAA 
CACAGCCTGA ATTTACAGGT GGATGGGCGG AGCTACATCA CCCCCAACCC TTACCCACAA 
TCCACCTCGG CTGAGACGAA AAACCCAGTT TACCTGGGAG GCTACCCTGT CGGGGTAAAG 
CAGAACTGCC TGTCAATCAC CACCCGGTTC AGAGGTTGTC TGAAGAAGAT ACAGCTCATT 
AAACCGCACC TGAGCGATAC GCTGGACCTA AGCTCCGCCC ACGTCCTCTC AGGTGTCACT 
CCTGACTCCT GCCCTGTAGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lama1 ( XM_029842715.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029698575.1 Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X4, mRNA.

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CloneID	OTo43640
Clone ID Related Accession (Same CDS sequence)	XM_029842715.1
Accession Version	XM_029842715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	laminin subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGC TGCTGTTGGT GCTGCTGCTG GATGGAGCTG CAGCTCAGCT GAGAGGCCTG 
TTTCCTGCCA TCTTGAACTT GGCCACCAAC GCCGTGGTCG ACAGCAACGC CACGTGCGGC 
GACCCGGACC CGGAGGTCTA CTGTAAACTG GTGGAGCACG TTCCGGGTCG ACCCGTAAAG 
AACCCTCACT GCGCACGCTG TGACGCCGCC TCGCTTCTGC TGAGAGAACG ACATCCCATC 
ACCAACGCCA TCGACGGAAC CAACCGCTGG TGGCAGAGTC CCAGTATCAA GAATGGGCGG 
CAGTTTCACT GGGTCACCAT CACGCTGGAC CTGAAACAGA TCTTCCAGGT CGCCTACGTC 
ATCATCAAAG CAGCGAACTC TCCTCGCCCA GGTAACTGGA TCCTGGAGCG CTCCCTGGAC 
GGGGTGACCT TTGACCCCTG GCAGTATTAC GCAATTAGCA ACTCCGAGTG CTTGTCCCAT 
TACAACGTCA CCCCGAGACT CGGACCTCCG ACCTACAAGA GGGACACGGA GGTCATCTGC 
ACGTCCTACT ACTCCAGACT GAACCCTCTG GAGAACGGAG AGATCCACAC CTCGCTGATC 
AACGGCCGCC CCGGAGCGGA CGACCTGACG CCCGAGCTCT TGGACTTCAC CTCGGCCCGT 
TACATCCGAC TCAGGCTCCA GCGGATCAGG ACGCTCAACG CCGACCTCAT GATGCTGAGC 
ACGCGGGACC CCCGAGACAT CGACCCCATC GTCACACGGA GGTATTATTA CTCCATCAAA 
GATGTGTCCG TGGGTGGAAT GTGCATCTGT TATGGCCACG CCCAGAATTG CCCCTTGGAC 
CCGGTCACAA AGAAACTACA CTGTGTGTGC GAACACAACA CCTGTGGAGA GAACTGTAAC 
GAGTGTTGCC CTGGTTACCA TCAGCAGCCA TGGCAACCAG GAACGGTTCG TGAGGGAAAC 
ACCTGTGAAA AATGTAACTG TCACAACAAA GCAACCGACT GTTTCTACAA TCAGACGGTG 
GCCGACCTCT CTCTGAGTTT GAATATCCAC GGCGTCCGTC GAGGGGGCGG AGTCTGTGTC 
TCCTGTCTAC ACAACACCGC CGGGCTCAAC TGTGAGAGCT GCACTGATGG CTACTACAGA 
CCTGCTGAGG TCTCTCCGTA TTCAGACTTT CCGTGTGTTG CCTGTAACTG TGACCTGAGA 
GGATCAGAGT CACCTGTGTG CTCCAGGGAC GACAAGCATC CGGGTCAGTG TGTGTGTAAG 
GAGGGTTTCG CAGGTGAACG GTGCGACACC TGTGCGTTTG GATACAGAGA CTTTCCCCAG 
TGTGTTCGCT GTGAGTGTGA CGCTGCTGGC AGCACCAACG CGGACCCCTG CAGACCCTGC 
ACCTGCAAGG TGAACGTGAT GGGAGCCCAC TGTGATCTGT GTAAACCCGG ATTCTACAAC 
CTCCAGGAGA AGAATCCGCT CGGCTGCACC GACTGTTTCT GCTTCGGCGT GTCCGACGTC 
TGTGAGAGTT CTGCTTGGTC CACAACTCAG GTGCTTCATA GAGATGCTTG GCTCCTCCCC 
CGCCCGGCTC CTCAGAACAT TACAGTCCTC CAGGACAATG ATTTACCCTC ACCTGGAAAC 
ACTTCCTCTG GCCACTCCCA CCAGGACGTT CTGCTGTGGG ATGCACCTGA CGGTTTCCTT 
GGTAACAAGC TGGGCTCCTA TGGAGGCTTC CTGAACTACT CTCTGCTGTA TGACATTCCA 
TCGGACAATG AGGACCGCTC TATTCCCGCC CACTCTGACG TCATGATCAA GGGAAACGGT 
CGTGCTCTAC ATTCCATCCC TCACTTCCTC TTTGTCTCTC CTCTTGCTGA GAGTGTGGTT 
GCCATAGTGA TGGTTGACGG GCAGTTCAGG GATGTTCAGT CAGGCGACCC CGTGAGCCGC 
GATGAGCTCC TGTCGGCCCT CGCCGACGTT ACGACTCTGA CCGTCAGGGT CGGTCTGAAC 
GCCACCGCAG ATGGACCGAT ACGTCTCAGC TGGATCTCCC TGGACGCCGC TGACCCGGGC 
CCAGGTTCTG CCCTGGACGC CGCTGACCCG GGCCCAGGTT CTGCCCAAGA GGCGGTCGCC 
GTGGAAAAGT GTGAATGTCC GTGGGGTTAC ACCGGCACTT CCTGTGAGAC GTGTCTCCCG 
GGGTTCTACA GAGTGGGCGG GGTTTTGTTC GGAGGAAACT GTATGCAGTG CGAGTGTAAC 
GACCACGCGT CAGAGTGTGA CATCAGTGGC GACTGTTGGG GTTGTACACA TAACACCAGC 
GGTCCTCACT GTGAAGAGTG TCTCCCTGGT TTCTATGGCA ACGCCACAGA GGGGACAGCA 
GATGACTGCC AGTTGTGTCC TTGTCCTCTG ACAAACCCGT CCAACAGTTT CAGTCCCACC 
TGCGTGCTGG AAGGGTCAGG AAAGGTGGTG TGTGACCAGT GCCAGGAGGG ACACACAGGA 
AGCAACTGTG AAAGGTGTGC GAGTGGTTTC TATGGTAACC CACGGGCAGC CAATGGCACC 
TGTGTGCGCT GTGATTGTCA CGGTAATGTG AACGCAAGTG AGGCGGGACA CTGTGACGTC 
ATCACCGGCG AGTGCCTGCG GTGCCTTTTC AACACCGGCG GACGCCACTG CGAGGTCTGT 
CAGCCCGGTT ACTATGGAGA CGCAGTGCTT GCCAAGAACT GTCAGGAATG TGGCTGTGAC 
GTCAACGGCG CCCCCTCCAG TGTGTGTGAC GTCACAACGG GTCAATGTCT TTGCAAGGAA 
AACGTGACAG GACGCACTTG TGACCAGTGT CAGTCGGGCT TTTTCGGCCT CCGCAGTGAG 
ATGGGCTGTC AGCTCTGTGG CTGCAACCAA TCGGGATCGC TGTCTGAGTC ATGTGACGAG 
CAGGGCCGCT GCCCGTGCGT GAAGGGTGTG GGGGGAGACA AGTGTGACCG CTGTGGTCAC 
GGCTACTACG GCGTGGACGT CAGCAGCTGC AGAGCATGCA ACTGTGTCGA CACGGGAGGG 
AACTGTGATC CCCAGAGCGG GGAGTGCATG TGCCCCGCCT TTACAGAGGG ACCCAACTGC 
AACAGATGTG AAACTGGATA CTGGGGTCAC GACCCCACAA CTGGCTGCAC GCCGTGCAGC 
TGCAGCAAGG ACGGAAGCTC CGCCCCTGAC TGTGATGCGA CCAGCGGCCA GTGTTGGTGC 
AGGAAGGGGT TTGGTGGCCG GTCATGTGAC CACTGTGCCG CTGGGCACCA TGGTTACCCG 
TCATGTTTAC CATGCGGCTG TGACCTGGCA GGAACTGATG AAAAGTTCTG CAACACGACG 
CTGAACACGT GTGACTGTCT GGACACGGGA GTGTGTGTGT GCAAGGTGGG CGTGTCTGGG 
CGCCGCTGCG AGGAGTGTGT CGCCGGCCGA TTCGCTCTGT CGGTGGACAA TCCCGATGGC 
TGCTCTCCGT GTTTCTGTTC GGGGGTGAGC GGCGACTGTG AGGAGCAGGG GGGGCTGATC 
CGACTACCGG TCACTCTGGA CCAGTCTCCT GGCCTCTTGT CAGTGGTTAG TCAGTTGAAC 
TTGCAGGGTG TCGTGTCTGG AGTTTACCAG CAGGGCGGCG ACATGCTGCT CGACACCAGG 
CACCTCAACA CCAGTGGCTG GGCTGGCCCA CTCTACTGGA GACTGCCCCC CCAGTTTGAA 
GGCCAACAGC TTCTGTCCTA CGGCGGCTCC TTATCCTACA TCATCACCTT TTATGCTGAG 
GATGGCGCTG GGCTGTCCAA TCAGGAGCCC CAGATCATGA TAAAAGGTGG GACCCTCAAG 
AAGATGGTGA TATACATGGA CGTGGTTGCC CCTAGCAACG GCATTAGGAC AAACCATGAG 
ATCAGGATGA CGGAGCACAA GTGGAAATAC TTTAACTCAG TGTTGGAGAC GTCGGTGGGT 
CGCTCGGACT TCATGTCCGT CCTGGGCAAC ATCGAGTACG TCCTCATCAA AGCGTCTTAC 
GGGAGCAGGC TGCAGCAGAG CAGGATCTCA AATATTACCA TGGAAACGGC GCTGGAGAAC 
GAGGACGGGT CAGAGGTCGG CGGGGACATG GCAAGATCGA TCGAGTCCTG CATCTGTCCC 
ACGGGATACA CCGGACTGTC CTGTCAGGAC TGTGCCGCTG GATTCTTCCG TCAGCCGCTG 
TCCGAGTTGT CACCTCAGAG TCAAAGGTTG ATGTCGGTGC GTCCGTGCGT TCCGTGTCGC 
TGCAACAACC ACAGCGAGAG CTGTGATGCA GCGAGTGGAG AATGTCAGCA GTGTCAGCAT 
CACACCAGCG GGTGGAGCTG TCAGCAGTGT TCCCAAGGGT ATCACGGGAA CGTTAGCGGC 
TCCATAAGTG ACTGCTCTCT TTGCGCCTGC CCCCTGAAGG ACAACAGCTT CAGCCCCACC 
TGTGTGTCGG AGGGAGTGCT GGGAGACTTC CGCTGCACAG CCTGTGTTCC CGGATACGAG 
GGGAGATACT GTGAAAAGTG TTCCGTCGGT TACTATGGTA ACCCCACTTT GCCAGGCGGC 
GTGTGTTCAC GGTGTAACTG TTCTGGGTGG GGTTCTCTTC AGCCGCTGTG TGACCAGCTG 
AATGGGAAGT GCGACTGTAA GGCTGGTGTC AAAGGTCAAT CATGTGACCA GTGCGAGGAC 
AGACACATCC TGCAGGAAGG AGAGTGTGTG TCGTGTGAGG ACAACTGCAC CGGTGTGATG 
CTCAACGACC TGGAAGACTT GGACGACCAC CTCGCCGCCG TCAACATCAG CAGCTTTGTT 
GTGGCCTCGT ACCTTCGGTT AGCGGCGCTG GAGAACCAGA CCAGAGGCGT CCAGGCGATG 
GCGGCGGAAA ACACTTCAGC CTCTCTGCAG CTGAGCGGCC TCGAAGAGCA ACTCGGCCTC 
ATGAGCCTCG ACCTCGGCGT CTTGCTGCAG CTGGTGGTAA ATATGTCTGG TGACCTTCAG 
AGACTGGACA CATCTGCTCA GGACACCATC CATCAGGGCT CACAGCAAAT CCAGAACATC 
AACATCCTTC AAGACAAAGT GCAGGCTCTT CAGCAGGAGG CCGAACGCCT GAATGCGACT 
GCAGAAGAAG AGCTGACGAC AGCCAACAGA ACACATCTGC TGCAAGAACT TGAGTTGATG 
TTGAGAAGCA TGAGAGTGAC CAACCTCACC GCTGCCAGCA GCCTGGCCAA TCAGGAGCTC 
CTACGCGCCA CATCTGTCCT CTCCCTGCAG TCGGACCTGC TCTCCCGCAG GGCCGCAGTG 
GACGACGGCC TCCGCCACCT TTCGACCTCC CTCAGCAGTA ACAGCGGAAC ACTGCGCCAC 
CTACAGGCAC AACTGAGCGG CGCCACACAA CAAAACATGC AGACACACGT GCTTCTGGAG 
GTCCTGCACA ACCTGACGCA GCAGCACCAG TCTGTCCAGC AGGACATCAG CCAGGAGCGC 
CTCCTGGTGG CGGGACTGAT GCAGGACACT CAGCTCGTGC TGGGCGACAG CTTCAGCATC 
ATGGAGGACC TAACCAACAC CACCTCGCAG GTGGAGGTGT TTGGCAGTTA CCTGGATCAG 
TGGAGCCCCC TGCTGAGGAA ACGGGTGGAT GGTTTGGTTG GTGGTCTGAA GATGACGGAT 
TCCCTGGAGA AGGTCTACCT TTCAGAGAAA CACGCCCAGC AGCTGGACAA CGACGCCCAC 
TCCCTGCACA GCTCGCTGCT GTCTGTGTAT AATGCGTCTC AAAATGGCAG CCAGCTGCTT 
CAGGGGGGGA ACAGCGGCGT CCCGGAGCAG GTGGAGGCGC TGCGGCAGGA GGCCCTCATG 
GCCGACCGGT CCGCCCGGCT GCTGTTCAAC GCGACGGTCC AATCAGAACC GCCCCTGCCA 
GAGGAGAGCA GGACTCGCCT GAACATCAGC GCCGCTGTTT TGGAAACCTG TCGACAGATC 
GGCGCTTCAT CCGCAGACTT GCAGATGAAC GTCTCCATGG TAACCAGCAG ACTAGGATTA 
TTTAAAGAAA GCTTCCAGAA GTTCGGTCTC CTATTTCATC ATTCGATCAC AGAGCTGCAG 
AGCCTCTCAA ATGGTTCACT GACCGAGCTG CAACAAGTTC AGCTGCAGGC TGCAGAAGCA 
GACTCCAGGC TACAGGGGGC GCTACTGAGG CTACAGAAAC TCAGACAGCA GCTCCAGGAT 
TCTTCCTCTG CAGTGGAAAA CACCAGCAAC ACCGTCACAG AGACCCAGCA GCTCACAGCC 
CTCACACACT CCACAGTCGA TCAGGCGCGT CAGAAACTGG GCGAGGTGGA GCGTCGCACA 
GAGCGCCTTA TCGACCAGAT CAAGCCCCTG AGCATGCTGG GAGAAACGAT AAACAGGAAC 
CTGTCTGACA TCAGAGAGCT GATCAATCAG GCCCGAAGAC AGGCCGCCTC GATTAAGGTG 
GCGGTGCAGG CAGACAGAAG CTGCGTGCGC TCCTACAGGC CACAGTCCGA GTCCAGGAAC 
TTCAACACGC TGAGTCTTGT CTTGAGGACG TCCAGTCCAA ACAACCTGCT GTTCTTCCAG 
GGCAGCAACA CCACGGTGGA CTTCCTGGCT CTGGAGATGT ATCGTGGGAA GGTGTCTCTG 
CTGTGGGATC TGGGTTCAGG TAGCACCAGA CTGGAGTTTC CCGGACTGGA CATCACCAAC 
AACAGATGGA CCAGAATCAA CGCCACGCGG TTTGGGGCTC ATGCGTCTCT GTGGGTTCAT 
CAGCTGGAGT CGGACTCGGA GCCCCTCCCT GCAGTAACGG CCACCTCCCC GGGTCCCGCC 
AGGATTTTAG ACACGGACAG AAACTCTTGG CTCTACATTG GTGGACTGGG AGCTCACGGA 
CAGAGGCCGG CAGCCCTGCG CTCCACCACA TTCCAGGGAT GTCTGGGCGA AGCGGCCTTG 
AACGAGCGGA ACATCGGCCT CTGGAACTAC CACAGAAGGG AGGGAGAGTG TGGAGGCTGC 
TTCAGCAGTC CACAAACGGA GGAGACCTCC TTCCATTTCG ACGGATCCGG ATTCTCTTTG 
GTCCAGAAGC CTCTACGGGC GACCTCCACC TCCATCGTCT TGCTGTTCAA AACACTGTCA 
CCAGGGGGGT TACTGTTGTA CCTGGCCTCC AACAACACGA GGGACTTTCT GTCCATCGAG 
CTAGTAGAGG GGCAGGTTCG CCTGACCTTT GACCTCGGCT CAGGTGCACT CGTCCTGACC 
TCCAGCAGGA AGTACAACAC TGGCGTCTGG TACAAGATCA CGCTGCAGAG GAACAAACGT 
AAAGGTTACC TTTCCATCAT GGCTGCTGAC CAGTCGTCGG AAAAGGAGGT GTTGGAGGCG 
GAGTCTCCCG GGCTGGCGTC CGACCTTAAC CGCTCCGACT TTGACCCCAT CTACATCGGA 
GGACTTCCTG CCTCCAGACC CATCAGGCGA CAGGTGGTGT CCAGGGCTTA CGTGGGCTGT 
ATAAAAAATG TGGAGATCGC CCGGTCCAAC TTTGACCTGC TGAGAGACGC TTTTGGCGTC 
AGGAAGGGCT GCGTTCTTGA GGCCCTCAGG AGCGTGTCGC TGTTGACCGG CGGCTTCGTT 
CAAATGGCTC CTCGAGCTCT TGGACCAGAG GTGGAGCTTC TCTTTTCCTT CTCCACCAAC 
AACCGGTCAG GAATTCTGCT CGCTGCCGTC ACCGACGACC ACGTTCAGAG GCGGCGCTTC 
CTGTCCATCC ACCTGGCCTC AGGTGCGGTG GAGGCCGACA TTGGTGAAGT GGGAGGAGCC 
ACACAAACCG TGACAGTGGT GAAACCTAAG GGAGGATTTT TTAGCGATGG TCTGAAACAC 
TCTGTCATCG TTACCGTCAA CAAGAAGACC CTGTCCATAC AGGTGGATGA GACACACCTG 
AAGTCCATCC CTCTGTTGCC GGGCAGATTT TCTAATTTGT CTCCAGCCTC ATTTTTTATT 
GGTGGAGTAC CTGTAGGGGC GGGGCTCCAG CTGCCCTCCA GGCTGCGGCA GATTTCTTTA 
TCCTTCAGAG GCTGCATCCA ACACCTGGTG TTAGGAGGAG GAGCGCTCGT TGACCTCTCA 
GGAGCGTTGA GGTATGAGGG GGTGACCCTG GACAGCTGCC TGCTGGAAGA GGCGGCCAGG 
GGGGCGGTGC TTCCTGATGA CCCGGACACA GAACCAACCA TGGACCCGGT CCGCCTCCCT 
GTGGTACCGC CCACCCCGCT GGGTGTCCTG CCCCCAGGCG CTCTAACGTG TGCAGCACAG 
GTGGACTCCT CCTTCCTGCC CTCCGCAGCT CAGTTCGGTT TGTCCAAACA CAGTCACATG 
ACGTTCCTGA TCAACCCCGG CGCCCTCAGG AAAAGTGTGT CTCTGAAGAT ATCGGCTCGG 
ACCCGATCTC CGGACGGCCT GCTCCTCCTC CTCTCCGACG CCAAACAGAT GGACTTCGCC 
GTCGTCAAAC TAGCGGGGGG GAAGGTGACG TTGTCAGCCG ACCTGGGGAA GGGTCCCACC 
TCCGTCACCT CACCTGTGTC CATCACTGAC GGACTCTGGC ACACTGTGAG CGCCGAGGTC 
AGCCGCCGCT CGCTCGTCCT GGCGGTCGAC GGTTCAAAGC CCGGCTCGGC GTCCATCAGG 
GGGAACCAGC TGGACGTGGA CAGGCGCCTG TACCTGGGGG GGCTGCCGCA CGCACACGCC 
ACCAGGAGGA TTAACGTCAG CAGCAGCTTC CAGGGCTGCG TTCGGTCCGT CCACCTGAAC 
GGCGCCGTGT TGGATCTGAC CAAACCGGCC TCGCGCCACA ACGTCACTTC CTGTTTCACC 
GATGACCAGG CAGGAAGCTA CTTCGACGGC AGCGGACACG CCCTCTTCAT GCGCGATGGC 
TATAAAGTCG GATCCGATGT TTCTGTATCT CTGGAGTTCC GGACCAGCCA ATCAGAAGGA 
GTGTTTTTAG GAATTAGCAG TGCCAAAATT GATGCCATCG GACTCGAGAT GATCAAAGGA 
CAGGTGGTGT TTAACGTGAA CAATGGCGCC GGGAGAGTGA CGGTGCGCTC CGTCGGTCCG 
ATGCTGTGTG ACGGCCGATG GCACCGCGTC CTGGCCAGGA AAACCAAACA CAGCCTGAAT 
TTACAGGTGG ATGGGCGGAG CTACATCACC CCCAACCCTT ACCCACAATC CACCTCGGCT 
GAGACGAAAA ACCCAGTTTA CCTGGGAGGC TACCCTGTCG GGGTAAAGCA GAACTGCCTG 
TCAATCACCA CCCGGTTCAG AGGTTGTCTG AAGAAGATAC AGCTCATTAA ACCGCACCTG 
AGCGATACGC TGGACCTAAG CTCCGCCCAC GTCCTCTCAG GTGTCACTCC TGACTCCTGC 
CCTGTAGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029698575.1
CDS	90..9341
Translation	MKLLLLVLLLDGAAAQLRGLFPAILNLATNAVVDSNATCGDPDPEVYCKLVEHVPGRPVKNPHCARCDAASLLLRERHPITNAIDGTNRWWQSPSIKNGRQFHWVTITLDLKQIFQVAYVIIKAANSPRPGNWILERSLDGVTFDPWQYYAISNSECLSHYNVTPRLGPPTYKRDTEVICTSYYSRLNPLENGEIHTSLINGRPGADDLTPELLDFTSARYIRLRLQRIRTLNADLMMLSTRDPRDIDPIVTRRYYYSIKDVSVGGMCICYGHAQNCPLDPVTKKLHCVCEHNTCGENCNECCPGYHQQPWQPGTVREGNTCEKCNCHNKATDCFYNQTVADLSLSLNIHGVRRGGGVCVSCLHNTAGLNCESCTDGYYRPAEVSPYSDFPCVACNCDLRGSESPVCSRDDKHPGQCVCKEGFAGERCDTCAFGYRDFPQCVRCECDAAGSTNADPCRPCTCKVNVMGAHCDLCKPGFYNLQEKNPLGCTDCFCFGVSDVCESSAWSTTQVLHRDAWLLPRPAPQNITVLQDNDLPSPGNTSSGHSHQDVLLWDAPDGFLGNKLGSYGGFLNYSLLYDIPSDNEDRSIPAHSDVMIKGNGRALHSIPHFLFVSPLAESVVAIVMVDGQFRDVQSGDPVSRDELLSALADVTTLTVRVGLNATADGPIRLSWISLDAADPGPGSALDAADPGPGSAQEAVAVEKCECPWGYTGTSCETCLPGFYRVGGVLFGGNCMQCECNDHASECDISGDCWGCTHNTSGPHCEECLPGFYGNATEGTADDCQLCPCPLTNPSNSFSPTCVLEGSGKVVCDQCQEGHTGSNCERCASGFYGNPRAANGTCVRCDCHGNVNASEAGHCDVITGECLRCLFNTGGRHCEVCQPGYYGDAVLAKNCQECGCDVNGAPSSVCDVTTGQCLCKENVTGRTCDQCQSGFFGLRSEMGCQLCGCNQSGSLSESCDEQGRCPCVKGVGGDKCDRCGHGYYGVDVSSCRACNCVDTGGNCDPQSGECMCPAFTEGPNCNRCETGYWGHDPTTGCTPCSCSKDGSSAPDCDATSGQCWCRKGFGGRSCDHCAAGHHGYPSCLPCGCDLAGTDEKFCNTTLNTCDCLDTGVCVCKVGVSGRRCEECVAGRFALSVDNPDGCSPCFCSGVSGDCEEQGGLIRLPVTLDQSPGLLSVVSQLNLQGVVSGVYQQGGDMLLDTRHLNTSGWAGPLYWRLPPQFEGQQLLSYGGSLSYIITFYAEDGAGLSNQEPQIMIKGGTLKKMVIYMDVVAPSNGIRTNHEIRMTEHKWKYFNSVLETSVGRSDFMSVLGNIEYVLIKASYGSRLQQSRISNITMETALENEDGSEVGGDMARSIESCICPTGYTGLSCQDCAAGFFRQPLSELSPQSQRLMSVRPCVPCRCNNHSESCDAASGECQQCQHHTSGWSCQQCSQGYHGNVSGSISDCSLCACPLKDNSFSPTCVSEGVLGDFRCTACVPGYEGRYCEKCSVGYYGNPTLPGGVCSRCNCSGWGSLQPLCDQLNGKCDCKAGVKGQSCDQCEDRHILQEGECVSCEDNCTGVMLNDLEDLDDHLAAVNISSFVVASYLRLAALENQTRGVQAMAAENTSASLQLSGLEEQLGLMSLDLGVLLQLVVNMSGDLQRLDTSAQDTIHQGSQQIQNINILQDKVQALQQEAERLNATAEEELTTANRTHLLQELELMLRSMRVTNLTAASSLANQELLRATSVLSLQSDLLSRRAAVDDGLRHLSTSLSSNSGTLRHLQAQLSGATQQNMQTHVLLEVLHNLTQQHQSVQQDISQERLLVAGLMQDTQLVLGDSFSIMEDLTNTTSQVEVFGSYLDQWSPLLRKRVDGLVGGLKMTDSLEKVYLSEKHAQQLDNDAHSLHSSLLSVYNASQNGSQLLQGGNSGVPEQVEALRQEALMADRSARLLFNATVQSEPPLPEESRTRLNISAAVLETCRQIGASSADLQMNVSMVTSRLGLFKESFQKFGLLFHHSITELQSLSNGSLTELQQVQLQAAEADSRLQGALLRLQKLRQQLQDSSSAVENTSNTVTETQQLTALTHSTVDQARQKLGEVERRTERLIDQIKPLSMLGETINRNLSDIRELINQARRQAASIKVAVQADRSCVRSYRPQSESRNFNTLSLVLRTSSPNNLLFFQGSNTTVDFLALEMYRGKVSLLWDLGSGSTRLEFPGLDITNNRWTRINATRFGAHASLWVHQLESDSEPLPAVTATSPGPARILDTDRNSWLYIGGLGAHGQRPAALRSTTFQGCLGEAALNERNIGLWNYHRREGECGGCFSSPQTEETSFHFDGSGFSLVQKPLRATSTSIVLLFKTLSPGGLLLYLASNNTRDFLSIELVEGQVRLTFDLGSGALVLTSSRKYNTGVWYKITLQRNKRKGYLSIMAADQSSEKEVLEAESPGLASDLNRSDFDPIYIGGLPASRPIRRQVVSRAYVGCIKNVEIARSNFDLLRDAFGVRKGCVLEALRSVSLLTGGFVQMAPRALGPEVELLFSFSTNNRSGILLAAVTDDHVQRRRFLSIHLASGAVEADIGEVGGATQTVTVVKPKGGFFSDGLKHSVIVTVNKKTLSIQVDETHLKSIPLLPGRFSNLSPASFFIGGVPVGAGLQLPSRLRQISLSFRGCIQHLVLGGGALVDLSGALRYEGVTLDSCLLEEAARGAVLPDDPDTEPTMDPVRLPVVPPTPLGVLPPGALTCAAQVDSSFLPSAAQFGLSKHSHMTFLINPGALRKSVSLKISARTRSPDGLLLLLSDAKQMDFAVVKLAGGKVTLSADLGKGPTSVTSPVSITDGLWHTVSAEVSRRSLVLAVDGSKPGSASIRGNQLDVDRRLYLGGLPHAHATRRINVSSSFQGCVRSVHLNGAVLDLTKPASRHNVTSCFTDDQAGSYFDGSGHALFMRDGYKVGSDVSVSLEFRTSQSEGVFLGISSAKIDAIGLEMIKGQVVFNVNNGAGRVTVRSVGPMLCDGRWHRVLARKTKHSLNLQVDGRSYITPNPYPQSTSAETKNPVYLGGYPVGVKQNCLSITTRFRGCLKKIQLIKPHLSDTLDLSSAHVLSGVTPDSCPVG

Target ORF information:

RefSeq Version	XM_029842715.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes laminin subunit alpha 1 (lama1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029842715.1

ORF Insert Sequence:

ATGAAGTTGC TGCTGTTGGT GCTGCTGCTG GATGGAGCTG CAGCTCAGCT GAGAGGCCTG 
TTTCCTGCCA TCTTGAACTT GGCCACCAAC GCCGTGGTCG ACAGCAACGC CACGTGCGGC 
GACCCGGACC CGGAGGTCTA CTGTAAACTG GTGGAGCACG TTCCGGGTCG ACCCGTAAAG 
AACCCTCACT GCGCACGCTG TGACGCCGCC TCGCTTCTGC TGAGAGAACG ACATCCCATC 
ACCAACGCCA TCGACGGAAC CAACCGCTGG TGGCAGAGTC CCAGTATCAA GAATGGGCGG 
CAGTTTCACT GGGTCACCAT CACGCTGGAC CTGAAACAGA TCTTCCAGGT CGCCTACGTC 
ATCATCAAAG CAGCGAACTC TCCTCGCCCA GGTAACTGGA TCCTGGAGCG CTCCCTGGAC 
GGGGTGACCT TTGACCCCTG GCAGTATTAC GCAATTAGCA ACTCCGAGTG CTTGTCCCAT 
TACAACGTCA CCCCGAGACT CGGACCTCCG ACCTACAAGA GGGACACGGA GGTCATCTGC 
ACGTCCTACT ACTCCAGACT GAACCCTCTG GAGAACGGAG AGATCCACAC CTCGCTGATC 
AACGGCCGCC CCGGAGCGGA CGACCTGACG CCCGAGCTCT TGGACTTCAC CTCGGCCCGT 
TACATCCGAC TCAGGCTCCA GCGGATCAGG ACGCTCAACG CCGACCTCAT GATGCTGAGC 
ACGCGGGACC CCCGAGACAT CGACCCCATC GTCACACGGA GGTATTATTA CTCCATCAAA 
GATGTGTCCG TGGGTGGAAT GTGCATCTGT TATGGCCACG CCCAGAATTG CCCCTTGGAC 
CCGGTCACAA AGAAACTACA CTGTGTGTGC GAACACAACA CCTGTGGAGA GAACTGTAAC 
GAGTGTTGCC CTGGTTACCA TCAGCAGCCA TGGCAACCAG GAACGGTTCG TGAGGGAAAC 
ACCTGTGAAA AATGTAACTG TCACAACAAA GCAACCGACT GTTTCTACAA TCAGACGGTG 
GCCGACCTCT CTCTGAGTTT GAATATCCAC GGCGTCCGTC GAGGGGGCGG AGTCTGTGTC 
TCCTGTCTAC ACAACACCGC CGGGCTCAAC TGTGAGAGCT GCACTGATGG CTACTACAGA 
CCTGCTGAGG TCTCTCCGTA TTCAGACTTT CCGTGTGTTG CCTGTAACTG TGACCTGAGA 
GGATCAGAGT CACCTGTGTG CTCCAGGGAC GACAAGCATC CGGGTCAGTG TGTGTGTAAG 
GAGGGTTTCG CAGGTGAACG GTGCGACACC TGTGCGTTTG GATACAGAGA CTTTCCCCAG 
TGTGTTCGCT GTGAGTGTGA CGCTGCTGGC AGCACCAACG CGGACCCCTG CAGACCCTGC 
ACCTGCAAGG TGAACGTGAT GGGAGCCCAC TGTGATCTGT GTAAACCCGG ATTCTACAAC 
CTCCAGGAGA AGAATCCGCT CGGCTGCACC GACTGTTTCT GCTTCGGCGT GTCCGACGTC 
TGTGAGAGTT CTGCTTGGTC CACAACTCAG GTGCTTCATA GAGATGCTTG GCTCCTCCCC 
CGCCCGGCTC CTCAGAACAT TACAGTCCTC CAGGACAATG ATTTACCCTC ACCTGGAAAC 
ACTTCCTCTG GCCACTCCCA CCAGGACGTT CTGCTGTGGG ATGCACCTGA CGGTTTCCTT 
GGTAACAAGC TGGGCTCCTA TGGAGGCTTC CTGAACTACT CTCTGCTGTA TGACATTCCA 
TCGGACAATG AGGACCGCTC TATTCCCGCC CACTCTGACG TCATGATCAA GGGAAACGGT 
CGTGCTCTAC ATTCCATCCC TCACTTCCTC TTTGTCTCTC CTCTTGCTGA GAGTGTGGTT 
GCCATAGTGA TGGTTGACGG GCAGTTCAGG GATGTTCAGT CAGGCGACCC CGTGAGCCGC 
GATGAGCTCC TGTCGGCCCT CGCCGACGTT ACGACTCTGA CCGTCAGGGT CGGTCTGAAC 
GCCACCGCAG ATGGACCGAT ACGTCTCAGC TGGATCTCCC TGGACGCCGC TGACCCGGGC 
CCAGGTTCTG CCCTGGACGC CGCTGACCCG GGCCCAGGTT CTGCCCAAGA GGCGGTCGCC 
GTGGAAAAGT GTGAATGTCC GTGGGGTTAC ACCGGCACTT CCTGTGAGAC GTGTCTCCCG 
GGGTTCTACA GAGTGGGCGG GGTTTTGTTC GGAGGAAACT GTATGCAGTG CGAGTGTAAC 
GACCACGCGT CAGAGTGTGA CATCAGTGGC GACTGTTGGG GTTGTACACA TAACACCAGC 
GGTCCTCACT GTGAAGAGTG TCTCCCTGGT TTCTATGGCA ACGCCACAGA GGGGACAGCA 
GATGACTGCC AGTTGTGTCC TTGTCCTCTG ACAAACCCGT CCAACAGTTT CAGTCCCACC 
TGCGTGCTGG AAGGGTCAGG AAAGGTGGTG TGTGACCAGT GCCAGGAGGG ACACACAGGA 
AGCAACTGTG AAAGGTGTGC GAGTGGTTTC TATGGTAACC CACGGGCAGC CAATGGCACC 
TGTGTGCGCT GTGATTGTCA CGGTAATGTG AACGCAAGTG AGGCGGGACA CTGTGACGTC 
ATCACCGGCG AGTGCCTGCG GTGCCTTTTC AACACCGGCG GACGCCACTG CGAGGTCTGT 
CAGCCCGGTT ACTATGGAGA CGCAGTGCTT GCCAAGAACT GTCAGGAATG TGGCTGTGAC 
GTCAACGGCG CCCCCTCCAG TGTGTGTGAC GTCACAACGG GTCAATGTCT TTGCAAGGAA 
AACGTGACAG GACGCACTTG TGACCAGTGT CAGTCGGGCT TTTTCGGCCT CCGCAGTGAG 
ATGGGCTGTC AGCTCTGTGG CTGCAACCAA TCGGGATCGC TGTCTGAGTC ATGTGACGAG 
CAGGGCCGCT GCCCGTGCGT GAAGGGTGTG GGGGGAGACA AGTGTGACCG CTGTGGTCAC 
GGCTACTACG GCGTGGACGT CAGCAGCTGC AGAGCATGCA ACTGTGTCGA CACGGGAGGG 
AACTGTGATC CCCAGAGCGG GGAGTGCATG TGCCCCGCCT TTACAGAGGG ACCCAACTGC 
AACAGATGTG AAACTGGATA CTGGGGTCAC GACCCCACAA CTGGCTGCAC GCCGTGCAGC 
TGCAGCAAGG ACGGAAGCTC CGCCCCTGAC TGTGATGCGA CCAGCGGCCA GTGTTGGTGC 
AGGAAGGGGT TTGGTGGCCG GTCATGTGAC CACTGTGCCG CTGGGCACCA TGGTTACCCG 
TCATGTTTAC CATGCGGCTG TGACCTGGCA GGAACTGATG AAAAGTTCTG CAACACGACG 
CTGAACACGT GTGACTGTCT GGACACGGGA GTGTGTGTGT GCAAGGTGGG CGTGTCTGGG 
CGCCGCTGCG AGGAGTGTGT CGCCGGCCGA TTCGCTCTGT CGGTGGACAA TCCCGATGGC 
TGCTCTCCGT GTTTCTGTTC GGGGGTGAGC GGCGACTGTG AGGAGCAGGG GGGGCTGATC 
CGACTACCGG TCACTCTGGA CCAGTCTCCT GGCCTCTTGT CAGTGGTTAG TCAGTTGAAC 
TTGCAGGGTG TCGTGTCTGG AGTTTACCAG CAGGGCGGCG ACATGCTGCT CGACACCAGG 
CACCTCAACA CCAGTGGCTG GGCTGGCCCA CTCTACTGGA GACTGCCCCC CCAGTTTGAA 
GGCCAACAGC TTCTGTCCTA CGGCGGCTCC TTATCCTACA TCATCACCTT TTATGCTGAG 
GATGGCGCTG GGCTGTCCAA TCAGGAGCCC CAGATCATGA TAAAAGGTGG GACCCTCAAG 
AAGATGGTGA TATACATGGA CGTGGTTGCC CCTAGCAACG GCATTAGGAC AAACCATGAG 
ATCAGGATGA CGGAGCACAA GTGGAAATAC TTTAACTCAG TGTTGGAGAC GTCGGTGGGT 
CGCTCGGACT TCATGTCCGT CCTGGGCAAC ATCGAGTACG TCCTCATCAA AGCGTCTTAC 
GGGAGCAGGC TGCAGCAGAG CAGGATCTCA AATATTACCA TGGAAACGGC GCTGGAGAAC 
GAGGACGGGT CAGAGGTCGG CGGGGACATG GCAAGATCGA TCGAGTCCTG CATCTGTCCC 
ACGGGATACA CCGGACTGTC CTGTCAGGAC TGTGCCGCTG GATTCTTCCG TCAGCCGCTG 
TCCGAGTTGT CACCTCAGAG TCAAAGGTTG ATGTCGGTGC GTCCGTGCGT TCCGTGTCGC 
TGCAACAACC ACAGCGAGAG CTGTGATGCA GCGAGTGGAG AATGTCAGCA GTGTCAGCAT 
CACACCAGCG GGTGGAGCTG TCAGCAGTGT TCCCAAGGGT ATCACGGGAA CGTTAGCGGC 
TCCATAAGTG ACTGCTCTCT TTGCGCCTGC CCCCTGAAGG ACAACAGCTT CAGCCCCACC 
TGTGTGTCGG AGGGAGTGCT GGGAGACTTC CGCTGCACAG CCTGTGTTCC CGGATACGAG 
GGGAGATACT GTGAAAAGTG TTCCGTCGGT TACTATGGTA ACCCCACTTT GCCAGGCGGC 
GTGTGTTCAC GGTGTAACTG TTCTGGGTGG GGTTCTCTTC AGCCGCTGTG TGACCAGCTG 
AATGGGAAGT GCGACTGTAA GGCTGGTGTC AAAGGTCAAT CATGTGACCA GTGCGAGGAC 
AGACACATCC TGCAGGAAGG AGAGTGTGTG TCGTGTGAGG ACAACTGCAC CGGTGTGATG 
CTCAACGACC TGGAAGACTT GGACGACCAC CTCGCCGCCG TCAACATCAG CAGCTTTGTT 
GTGGCCTCGT ACCTTCGGTT AGCGGCGCTG GAGAACCAGA CCAGAGGCGT CCAGGCGATG 
GCGGCGGAAA ACACTTCAGC CTCTCTGCAG CTGAGCGGCC TCGAAGAGCA ACTCGGCCTC 
ATGAGCCTCG ACCTCGGCGT CTTGCTGCAG CTGGTGGTAA ATATGTCTGG TGACCTTCAG 
AGACTGGACA CATCTGCTCA GGACACCATC CATCAGGGCT CACAGCAAAT CCAGAACATC 
AACATCCTTC AAGACAAAGT GCAGGCTCTT CAGCAGGAGG CCGAACGCCT GAATGCGACT 
GCAGAAGAAG AGCTGACGAC AGCCAACAGA ACACATCTGC TGCAAGAACT TGAGTTGATG 
TTGAGAAGCA TGAGAGTGAC CAACCTCACC GCTGCCAGCA GCCTGGCCAA TCAGGAGCTC 
CTACGCGCCA CATCTGTCCT CTCCCTGCAG TCGGACCTGC TCTCCCGCAG GGCCGCAGTG 
GACGACGGCC TCCGCCACCT TTCGACCTCC CTCAGCAGTA ACAGCGGAAC ACTGCGCCAC 
CTACAGGCAC AACTGAGCGG CGCCACACAA CAAAACATGC AGACACACGT GCTTCTGGAG 
GTCCTGCACA ACCTGACGCA GCAGCACCAG TCTGTCCAGC AGGACATCAG CCAGGAGCGC 
CTCCTGGTGG CGGGACTGAT GCAGGACACT CAGCTCGTGC TGGGCGACAG CTTCAGCATC 
ATGGAGGACC TAACCAACAC CACCTCGCAG GTGGAGGTGT TTGGCAGTTA CCTGGATCAG 
TGGAGCCCCC TGCTGAGGAA ACGGGTGGAT GGTTTGGTTG GTGGTCTGAA GATGACGGAT 
TCCCTGGAGA AGGTCTACCT TTCAGAGAAA CACGCCCAGC AGCTGGACAA CGACGCCCAC 
TCCCTGCACA GCTCGCTGCT GTCTGTGTAT AATGCGTCTC AAAATGGCAG CCAGCTGCTT 
CAGGGGGGGA ACAGCGGCGT CCCGGAGCAG GTGGAGGCGC TGCGGCAGGA GGCCCTCATG 
GCCGACCGGT CCGCCCGGCT GCTGTTCAAC GCGACGGTCC AATCAGAACC GCCCCTGCCA 
GAGGAGAGCA GGACTCGCCT GAACATCAGC GCCGCTGTTT TGGAAACCTG TCGACAGATC 
GGCGCTTCAT CCGCAGACTT GCAGATGAAC GTCTCCATGG TAACCAGCAG ACTAGGATTA 
TTTAAAGAAA GCTTCCAGAA GTTCGGTCTC CTATTTCATC ATTCGATCAC AGAGCTGCAG 
AGCCTCTCAA ATGGTTCACT GACCGAGCTG CAACAAGTTC AGCTGCAGGC TGCAGAAGCA 
GACTCCAGGC TACAGGGGGC GCTACTGAGG CTACAGAAAC TCAGACAGCA GCTCCAGGAT 
TCTTCCTCTG CAGTGGAAAA CACCAGCAAC ACCGTCACAG AGACCCAGCA GCTCACAGCC 
CTCACACACT CCACAGTCGA TCAGGCGCGT CAGAAACTGG GCGAGGTGGA GCGTCGCACA 
GAGCGCCTTA TCGACCAGAT CAAGCCCCTG AGCATGCTGG GAGAAACGAT AAACAGGAAC 
CTGTCTGACA TCAGAGAGCT GATCAATCAG GCCCGAAGAC AGGCCGCCTC GATTAAGGTG 
GCGGTGCAGG CAGACAGAAG CTGCGTGCGC TCCTACAGGC CACAGTCCGA GTCCAGGAAC 
TTCAACACGC TGAGTCTTGT CTTGAGGACG TCCAGTCCAA ACAACCTGCT GTTCTTCCAG 
GGCAGCAACA CCACGGTGGA CTTCCTGGCT CTGGAGATGT ATCGTGGGAA GGTGTCTCTG 
CTGTGGGATC TGGGTTCAGG TAGCACCAGA CTGGAGTTTC CCGGACTGGA CATCACCAAC 
AACAGATGGA CCAGAATCAA CGCCACGCGG TTTGGGGCTC ATGCGTCTCT GTGGGTTCAT 
CAGCTGGAGT CGGACTCGGA GCCCCTCCCT GCAGTAACGG CCACCTCCCC GGGTCCCGCC 
AGGATTTTAG ACACGGACAG AAACTCTTGG CTCTACATTG GTGGACTGGG AGCTCACGGA 
CAGAGGCCGG CAGCCCTGCG CTCCACCACA TTCCAGGGAT GTCTGGGCGA AGCGGCCTTG 
AACGAGCGGA ACATCGGCCT CTGGAACTAC CACAGAAGGG AGGGAGAGTG TGGAGGCTGC 
TTCAGCAGTC CACAAACGGA GGAGACCTCC TTCCATTTCG ACGGATCCGG ATTCTCTTTG 
GTCCAGAAGC CTCTACGGGC GACCTCCACC TCCATCGTCT TGCTGTTCAA AACACTGTCA 
CCAGGGGGGT TACTGTTGTA CCTGGCCTCC AACAACACGA GGGACTTTCT GTCCATCGAG 
CTAGTAGAGG GGCAGGTTCG CCTGACCTTT GACCTCGGCT CAGGTGCACT CGTCCTGACC 
TCCAGCAGGA AGTACAACAC TGGCGTCTGG TACAAGATCA CGCTGCAGAG GAACAAACGT 
AAAGGTTACC TTTCCATCAT GGCTGCTGAC CAGTCGTCGG AAAAGGAGGT GTTGGAGGCG 
GAGTCTCCCG GGCTGGCGTC CGACCTTAAC CGCTCCGACT TTGACCCCAT CTACATCGGA 
GGACTTCCTG CCTCCAGACC CATCAGGCGA CAGGTGGTGT CCAGGGCTTA CGTGGGCTGT 
ATAAAAAATG TGGAGATCGC CCGGTCCAAC TTTGACCTGC TGAGAGACGC TTTTGGCGTC 
AGGAAGGGCT GCGTTCTTGA GGCCCTCAGG AGCGTGTCGC TGTTGACCGG CGGCTTCGTT 
CAAATGGCTC CTCGAGCTCT TGGACCAGAG GTGGAGCTTC TCTTTTCCTT CTCCACCAAC 
AACCGGTCAG GAATTCTGCT CGCTGCCGTC ACCGACGACC ACGTTCAGAG GCGGCGCTTC 
CTGTCCATCC ACCTGGCCTC AGGTGCGGTG GAGGCCGACA TTGGTGAAGT GGGAGGAGCC 
ACACAAACCG TGACAGTGGT GAAACCTAAG GGAGGATTTT TTAGCGATGG TCTGAAACAC 
TCTGTCATCG TTACCGTCAA CAAGAAGACC CTGTCCATAC AGGTGGATGA GACACACCTG 
AAGTCCATCC CTCTGTTGCC GGGCAGATTT TCTAATTTGT CTCCAGCCTC ATTTTTTATT 
GGTGGAGTAC CTGTAGGGGC GGGGCTCCAG CTGCCCTCCA GGCTGCGGCA GATTTCTTTA 
TCCTTCAGAG GCTGCATCCA ACACCTGGTG TTAGGAGGAG GAGCGCTCGT TGACCTCTCA 
GGAGCGTTGA GGTATGAGGG GGTGACCCTG GACAGCTGCC TGCTGGAAGA GGCGGCCAGG 
GGGGCGGTGC TTCCTGATGA CCCGGACACA GAACCAACCA TGGACCCGGT CCGCCTCCCT 
GTGGTACCGC CCACCCCGCT GGGTGTCCTG CCCCCAGGCG CTCTAACGTG TGCAGCACAG 
GTGGACTCCT CCTTCCTGCC CTCCGCAGCT CAGTTCGGTT TGTCCAAACA CAGTCACATG 
ACGTTCCTGA TCAACCCCGG CGCCCTCAGG AAAAGTGTGT CTCTGAAGAT ATCGGCTCGG 
ACCCGATCTC CGGACGGCCT GCTCCTCCTC CTCTCCGACG CCAAACAGAT GGACTTCGCC 
GTCGTCAAAC TAGCGGGGGG GAAGGTGACG TTGTCAGCCG ACCTGGGGAA GGGTCCCACC 
TCCGTCACCT CACCTGTGTC CATCACTGAC GGACTCTGGC ACACTGTGAG CGCCGAGGTC 
AGCCGCCGCT CGCTCGTCCT GGCGGTCGAC GGTTCAAAGC CCGGCTCGGC GTCCATCAGG 
GGGAACCAGC TGGACGTGGA CAGGCGCCTG TACCTGGGGG GGCTGCCGCA CGCACACGCC 
ACCAGGAGGA TTAACGTCAG CAGCAGCTTC CAGGGCTGCG TTCGGTCCGT CCACCTGAAC 
GGCGCCGTGT TGGATCTGAC CAAACCGGCC TCGCGCCACA ACGTCACTTC CTGTTTCACC 
GATGACCAGG CAGGAAGCTA CTTCGACGGC AGCGGACACG CCCTCTTCAT GCGCGATGGC 
TATAAAGTCG GATCCGATGT TTCTGTATCT CTGGAGTTCC GGACCAGCCA ATCAGAAGGA 
GTGTTTTTAG GAATTAGCAG TGCCAAAATT GATGCCATCG GACTCGAGAT GATCAAAGGA 
CAGGTGGTGT TTAACGTGAA CAATGGCGCC GGGAGAGTGA CGGTGCGCTC CGTCGGTCCG 
ATGCTGTGTG ACGGCCGATG GCACCGCGTC CTGGCCAGGA AAACCAAACA CAGCCTGAAT 
TTACAGGTGG ATGGGCGGAG CTACATCACC CCCAACCCTT ACCCACAATC CACCTCGGCT 
GAGACGAAAA ACCCAGTTTA CCTGGGAGGC TACCCTGTCG GGGTAAAGCA GAACTGCCTG 
TCAATCACCA CCCGGTTCAG AGGTTGTCTG AAGAAGATAC AGCTCATTAA ACCGCACCTG 
AGCGATACGC TGGACCTAAG CTCCGCCCAC GTCCTCTCAG GTGTCACTCC TGACTCCTGC 
CCTGTAGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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lama1 cDNA ORF clone, Takifugu rubripes(torafugu)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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