pola1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	pola1
Entrez Gene ID	101069062
Full Name	DNA polymerase alpha 1, catalytic subunit
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003967875.2	XP_003967924.1	DNA polymerase alpha catalytic subunit
XM_003967875.3	XP_003967924.1	DNA polymerase alpha catalytic subunit

Pathways from BioSystems
Gene Ontology

The following pola1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pola1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo06822	XM_003967875.2 Latest version!	Takifugu rubripes polymerase (DNA directed), alpha 1, catalytic subunit (pola1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo43915	XM_003967875.3 Latest version!	Takifugu rubripes DNA polymerase alpha 1, catalytic subunit (pola1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pola1 ( XM_003967875.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003967924.1 Takifugu rubripes polymerase (DNA directed), alpha 1, catalytic subunit (pola1), mRNA.

Price & Availability↑

CloneID	OTo06822
Clone ID Related Accession (Same CDS sequence)	XM_003967875.2
Accession Version	XM_003967875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071023.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003967875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCGG TGTCACACCC AGACAAAGAC GTGGACGCTC CGGATGGCGA TGATGGAGGC 
GATACCTGCG GCCTGGCAAA GTCTCGCTCG AGACGGGAGA AGAGAGCAAA GTCCGGGAGA 
ATGTCGGCCC TAGATCAGCT CAAGAAAGCC AAGAAGGGAG AGAAAATCAA ATATAAGGTG 
GAGGAGTTGA CAAGTGTGTA TGAGGAGGTG GATGAAGAAC AGTATTCAAA GCTTGTTCGA 
GACCGACAAG AAGACGACTG GATTGTTGAT GATGATGGAA TAGGCTATGT TGAGGATGGA 
AGGGAGATCT TTGACGATGA CCTGGAAGAA GACATTGTGG AAAACAAAGG GAGAGCTGGT 
GCTAAAGGAG CAGACGCAAA GAAAAACATG AAGAAATGTA TTGTTGCCAA GCCGAACACC 
ATTAAGAGCC TGTTCATGAA CAGCAATGTA AAAAGACCTG CTGAGAAAGA TGTGGATTTA 
TCCAAAGATG AGCTGTTAGG AGATATTTTG CAAGACCTGC ACTCTGAGAA AAAAACTCTT 
CTGACTCCTC CACCAGTTGT CACTCTTAAG AAAAAGAAGT CTCATGGATC CCCCATGAAC 
CCCTTCTCCA TAAAACCTCA GATGTCAAAG GAGTCTATGG GGTCAAAATC CAAGGTCATC 
AAGCCACCAC CAGCTAATAG CACAGCCAGA CCGTCCATCC GTCACCCCTC CTCCATCCCG 
GCTCCTCCAA CAGAGAGACA CAAGACAAAA GAGGAGGAGC TAGAAACTGA AGTGAGTCAC 
ACACTGGACT TTGACGAGGT CGACTTCGAT GAGCTGATGG AGGTCGAGCT CGAGGAGGAG 
AAACCTGTGG TAAAGGATGA AGCTAAAAGT AAAGGAGAAG CAAAGGTGGC GCCTAAAGTG 
GAGCCTAAAA TGGAACCCCA TGATCCTATT CTATTCTCCA GCAAGACTGA AAGTTCGTGG 
GAACAAGAGG AAGAAGACAG GGCCAGCGCG GTTCCAGTGG ATATCCAAGT GGACACCAGC 
CAGCTCCCCC TGGTGGAGGA GCCAGATGGG GAGCAGGTGT TCCGCTTCTA TTGGTTGGAT 
GCCTTCGAAG AGCCATACAG CCATCCAGGT GTGGTGTACC TGTTTGGAAA AGTGTGGATC 
GAGTCAGCCA AGTCTCACGT CAGCTGCTGT GTTACAGTCA GAAACATTGA GAGGACCATG 
TATCTCCTGC CTCGGGAGTA TAGAGTAAAC CCCAAAACTG GGGAGGTGTC TGACACGCCT 
GTAGGAATGA TGGATGTCTA CCAGGAGTTT AATGAGCTTT CCGAGAAGTT CAAGATCATG 
AAATACAAGT CAAAGAAAGT GGAGAAGAAC TACGCATTTG AGATTCCAGA CGTTTCCAGT 
CACGGCGAGT ACCTGGAAGT CAGATATTCT GCTGAGTTTC CTGCACTACC TTCAGACCTG 
AAGGCCTCAA CATTTTCTCA CATATTTGGG ACCAACACCT CCTTTCTGGA GCACCTGCTG 
CTCAGCAGGA AAATTAAAGG CCCGTGTTGG CTCGACGTCA CGACGCCACA GCGCATGAGT 
CAGCCCGTCA GCTGGTGTAA GGTGGAGGCT CTGGCCCTGA AGCCTGACTT GATCAGTGTG 
ATCAAAGACC TGGTGCCCCC ACCCCTCACC GTCATGTCCA TCAGCCTCAA GACGGTGCAG 
AACCCCAAAA CGCTCCACAA CGAGATCGTG TCCTTGGCAG CTCTCATCCA CCATCAGTTC 
CAGATGGACA AAGCTGCGCC CGAGCCTCCA TACCAGTCAC ATTTTTGCGT CATCAGTAAA 
CCATCAGACT GTATCTTCCC ATATGATTTC AAAGAGGCCG TGAGGAAGAA GAACGGAAAA 
GTGGAAATAG CCCAGACAGA GCGAACTCTG CTCGGCTTCT TCTTGGCAAA GATGCACAAA 
ATTGACCCTG ATGTGCTGGT GGGTCACGAC ATCTTTGGTT TCGATTTAGA AGTGATTTTG 
CAGAGAATCA GCGTTTGCAA AGTCCCCCAC TGGTCTAAAA TTGGACGTCT CCGTCGGGCC 
AATATGCCCA AATTAGGGGG TCGCAGCGCC TTCGCAGAGA AGAGCGCCAC CTGTGGCCGC 
CTGGTGTGTG ATGTGGAGAT CTCAGCCAAG GAGCTAATAC GCTGTAAAAG TTACCACCTG 
ACTGAACTGA CCGCACACAT CCTGAAGGTG GAGAGAGTCA CGGTTCCTCA AGAAAACATC 
AGAAATCTTT ACAGCAACTC CCCTCACCTG CTTCGCCTGC TGGAGTTGAC GTGGACGGAC 
GCCAAGTTGA TCCTGCAGAT GATGTGTGAA CTGAATGTGT TGCCTCTGGC CCTGCAGATC 
ACCAACATCG CTGGCAACGT CATGTCCCGG ACTCTGATGG GCGGTCGCTC TGAGAGGAAC 
GAGTTTCTCC TGCTTCACGC CTTCCATGAG AAAAACTACA TTGTTCCTGA TAAACCCTCC 
TTCAAAAAGA CCCAGCTGGA AATGGCTGAA GGAGAAGACG AAGGTGCTGT GGGGAAAGGC 
AAGCGGAAGA AAAAGGCCGC CTATGCTGGA GGTTTGGTGC TGGATCCCAA AGTTGGTTTT 
TATGACAAGT TTGTGCTGTT GCTTGACTTC AACAGCCTGT ACCCCTCAAT CATCCAGGAG 
TTCAATATCT GCTTCACCAC TGTGCAGAGA GAGGCTCTCA ACTTGCAGAG AAAGAATGAG 
GAAGAGGATC AAGAAGAGAT TCCCGAGATT CCAGATTCAA GCCTGGAGAT GGGAATTCTC 
CCCAAAGAGA TTAGGAAGCT GGTGGAGCGA CGTAAACAAG TCAAACAGCT GATGAAACAG 
CAGGACATCA ACCCAGACGC CTACCTGCAG TATGACATCC GGCAGAAAGC TCTGAAGTTG 
ACTGCTAACA GCATGTACGG ATGTTTGGGC TTCAGCTATA GCCGCTTCTA CGCCAAGCCA 
CTCGCCGCCC TCGTCACTCA CAAAGGCAGA GAGATTTTGA TGCACACTAA AGAGATGGTT 
CAAAAGATGA ATCTGGATGT CATCTATGGC GATACAGACT CCATTATGAT AAACACCAAC 
AGCAAATCTT TGGAAGAGGT CTTCAAACTT GGCAATAAGG TGAAGGCTGA AGTAAACAAG 
CTCTACAAGC TCCTGGAGAT CGACATTGAC GGCGTGTTTA AGTCACTTCT TCTGCTGAAG 
AAGAAGAAGT ACGCAGCTTT GGTTGTGGAA AACCATGGCG ATGGACGCTA CACCTTGAAA 
CAGGAGCTCA AAGGTTTGGA CATCGTGCGC AGAGATTGGT GCGACCTGGC CAAAGAATGT 
GGCAACTATG TGATTGGTCA GATACTGTCG GATCAGAGCC GTGACGTGAT TGTGGAGAAT 
ATCCAGAAAC ATCTGGTGGA AATGGGGGAG AAGGTAGCAG CTGGAGACAT ACCACTAAAC 
CAGTATGAGA TACACAAGGC TCTGGCTAAA GATCCTCAGG ACTATCCAGA CAAGAAAAGC 
CTCCCTCATG TCCACGTGGC ACTGTGGATC AACTCTCAGG GTGGTCGCCG CGTCAAAGCT 
GGAGATACGG TCTCTTACAT CATTTGTAAG GACGGCTCCA CGCTGGCAGC CAGTCAGCGA 
GCTTACACAC AAGAGCAGCT CCAGAAACAG GAGGGTCTCA GTCTGGACAC TCAGTACTAC 
CTTGCCCAGC AGGTCCACCC TGTGGTGTCC CGAATCTGTG ACCCCATCGA GGGCATCGAT 
GGAGTGCTCA TAGCTACATG GCTGGGTCTG GATCCCAGTC AGTTCCGGGC TCAGCAGCAG 
TACCAGAGGG AGGACGAGAA TCACCTGCTG GGAGTTTCTC TCCAGCTGAC CGACGAGGAA 
CGTTACAAGG ACTGCGAGAA TTTCACCTTC ACCTGCCCAC AGTGCGGCAC AGACAACATC 
TGTGACAGTG TTTTTGAAGG AACCGGATTA AACCTGGAGC CCAGCTTGAT GCGATGCTGC 
CACATCCCCT GTGAGAGTCG GCCCATTGAC TACTCTGTCA ACATCTGCAA CAAACTGCTG 
CTCGACATCC GTCGACACAT CAAAAAATAC TACGCTGGCT GGTTGGTGTG TGAGGACCAG 
GCCTGCCAGA ACAGGACCAG ACGTTTGCCC ATTGCCTTCT CTCGCCAGGG ACCCATCTGC 
CCCGCCTGCT CCAGAGCCAC CCTCAGACCT GAGTATTCTG AGAAAGCTCT GTACAACCAG 
CTCTGCTTCT ATCGCTTCAT CTTCGACTGG GAGGCTGCAT TTTCCAAAAT CCAGGCTGAT 
GAGAAGAGTC ACGTGAAGAA CTGTAACAAA GAAAAAGAGG TGTACCGCCG GCTGAAAGAG 
GTGCCCGACA AAGCTTTAGC AGCCAGCAGC TACTCCGACA TTAACCTGGC CAAACTCTTC 
CAAGCGTTTA CCTCCCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003967924.1
CDS	117..4520
Translation	MAPVSHPDKDVDAPDGDDGGDTCGLAKSRSRREKRAKSGRMSALDQLKKAKKGEKIKYKVEELTSVYEEVDEEQYSKLVRDRQEDDWIVDDDGIGYVEDGREIFDDDLEEDIVENKGRAGAKGADAKKNMKKCIVAKPNTIKSLFMNSNVKRPAEKDVDLSKDELLGDILQDLHSEKKTLLTPPPVVTLKKKKSHGSPMNPFSIKPQMSKESMGSKSKVIKPPPANSTARPSIRHPSSIPAPPTERHKTKEEELETEVSHTLDFDEVDFDELMEVELEEEKPVVKDEAKSKGEAKVAPKVEPKMEPHDPILFSSKTESSWEQEEEDRASAVPVDIQVDTSQLPLVEEPDGEQVFRFYWLDAFEEPYSHPGVVYLFGKVWIESAKSHVSCCVTVRNIERTMYLLPREYRVNPKTGEVSDTPVGMMDVYQEFNELSEKFKIMKYKSKKVEKNYAFEIPDVSSHGEYLEVRYSAEFPALPSDLKASTFSHIFGTNTSFLEHLLLSRKIKGPCWLDVTTPQRMSQPVSWCKVEALALKPDLISVIKDLVPPPLTVMSISLKTVQNPKTLHNEIVSLAALIHHQFQMDKAAPEPPYQSHFCVISKPSDCIFPYDFKEAVRKKNGKVEIAQTERTLLGFFLAKMHKIDPDVLVGHDIFGFDLEVILQRISVCKVPHWSKIGRLRRANMPKLGGRSAFAEKSATCGRLVCDVEISAKELIRCKSYHLTELTAHILKVERVTVPQENIRNLYSNSPHLLRLLELTWTDAKLILQMMCELNVLPLALQITNIAGNVMSRTLMGGRSERNEFLLLHAFHEKNYIVPDKPSFKKTQLEMAEGEDEGAVGKGKRKKKAAYAGGLVLDPKVGFYDKFVLLLDFNSLYPSIIQEFNICFTTVQREALNLQRKNEEEDQEEIPEIPDSSLEMGILPKEIRKLVERRKQVKQLMKQQDINPDAYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHKGREILMHTKEMVQKMNLDVIYGDTDSIMINTNSKSLEEVFKLGNKVKAEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALVVENHGDGRYTLKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQSRDVIVENIQKHLVEMGEKVAAGDIPLNQYEIHKALAKDPQDYPDKKSLPHVHVALWINSQGGRRVKAGDTVSYIICKDGSTLAASQRAYTQEQLQKQEGLSLDTQYYLAQQVHPVVSRICDPIEGIDGVLIATWLGLDPSQFRAQQQYQREDENHLLGVSLQLTDEERYKDCENFTFTCPQCGTDNICDSVFEGTGLNLEPSLMRCCHIPCESRPIDYSVNICNKLLLDIRRHIKKYYAGWLVCEDQACQNRTRRLPIAFSRQGPICPACSRATLRPEYSEKALYNQLCFYRFIFDWEAAFSKIQADEKSHVKNCNKEKEVYRRLKEVPDKALAASSYSDINLAKLFQAFTSLK

Target ORF information:

RefSeq Version	XM_003967875.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes polymerase (DNA directed), alpha 1, catalytic subunit (pola1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003967875.2

ORF Insert Sequence:

ATGGCTCCGG TGTCACACCC AGACAAAGAC GTGGACGCTC CGGATGGCGA TGATGGAGGC 
GATACCTGCG GCCTGGCAAA GTCTCGCTCG AGACGGGAGA AGAGAGCAAA GTCCGGGAGA 
ATGTCGGCCC TAGATCAGCT CAAGAAAGCC AAGAAGGGAG AGAAAATCAA ATATAAGGTG 
GAGGAGTTGA CAAGTGTGTA TGAGGAGGTG GATGAAGAAC AGTATTCAAA GCTTGTTCGA 
GACCGACAAG AAGACGACTG GATTGTTGAT GATGATGGAA TAGGCTATGT TGAGGATGGA 
AGGGAGATCT TTGACGATGA CCTGGAAGAA GACATTGTGG AAAACAAAGG GAGAGCTGGT 
GCTAAAGGAG CAGACGCAAA GAAAAACATG AAGAAATGTA TTGTTGCCAA GCCGAACACC 
ATTAAGAGCC TGTTCATGAA CAGCAATGTA AAAAGACCTG CTGAGAAAGA TGTGGATTTA 
TCCAAAGATG AGCTGTTAGG AGATATTTTG CAAGACCTGC ACTCTGAGAA AAAAACTCTT 
CTGACTCCTC CACCAGTTGT CACTCTTAAG AAAAAGAAGT CTCATGGATC CCCCATGAAC 
CCCTTCTCCA TAAAACCTCA GATGTCAAAG GAGTCTATGG GGTCAAAATC CAAGGTCATC 
AAGCCACCAC CAGCTAATAG CACAGCCAGA CCGTCCATCC GTCACCCCTC CTCCATCCCG 
GCTCCTCCAA CAGAGAGACA CAAGACAAAA GAGGAGGAGC TAGAAACTGA AGTGAGTCAC 
ACACTGGACT TTGACGAGGT CGACTTCGAT GAGCTGATGG AGGTCGAGCT CGAGGAGGAG 
AAACCTGTGG TAAAGGATGA AGCTAAAAGT AAAGGAGAAG CAAAGGTGGC GCCTAAAGTG 
GAGCCTAAAA TGGAACCCCA TGATCCTATT CTATTCTCCA GCAAGACTGA AAGTTCGTGG 
GAACAAGAGG AAGAAGACAG GGCCAGCGCG GTTCCAGTGG ATATCCAAGT GGACACCAGC 
CAGCTCCCCC TGGTGGAGGA GCCAGATGGG GAGCAGGTGT TCCGCTTCTA TTGGTTGGAT 
GCCTTCGAAG AGCCATACAG CCATCCAGGT GTGGTGTACC TGTTTGGAAA AGTGTGGATC 
GAGTCAGCCA AGTCTCACGT CAGCTGCTGT GTTACAGTCA GAAACATTGA GAGGACCATG 
TATCTCCTGC CTCGGGAGTA TAGAGTAAAC CCCAAAACTG GGGAGGTGTC TGACACGCCT 
GTAGGAATGA TGGATGTCTA CCAGGAGTTT AATGAGCTTT CCGAGAAGTT CAAGATCATG 
AAATACAAGT CAAAGAAAGT GGAGAAGAAC TACGCATTTG AGATTCCAGA CGTTTCCAGT 
CACGGCGAGT ACCTGGAAGT CAGATATTCT GCTGAGTTTC CTGCACTACC TTCAGACCTG 
AAGGCCTCAA CATTTTCTCA CATATTTGGG ACCAACACCT CCTTTCTGGA GCACCTGCTG 
CTCAGCAGGA AAATTAAAGG CCCGTGTTGG CTCGACGTCA CGACGCCACA GCGCATGAGT 
CAGCCCGTCA GCTGGTGTAA GGTGGAGGCT CTGGCCCTGA AGCCTGACTT GATCAGTGTG 
ATCAAAGACC TGGTGCCCCC ACCCCTCACC GTCATGTCCA TCAGCCTCAA GACGGTGCAG 
AACCCCAAAA CGCTCCACAA CGAGATCGTG TCCTTGGCAG CTCTCATCCA CCATCAGTTC 
CAGATGGACA AAGCTGCGCC CGAGCCTCCA TACCAGTCAC ATTTTTGCGT CATCAGTAAA 
CCATCAGACT GTATCTTCCC ATATGATTTC AAAGAGGCCG TGAGGAAGAA GAACGGAAAA 
GTGGAAATAG CCCAGACAGA GCGAACTCTG CTCGGCTTCT TCTTGGCAAA GATGCACAAA 
ATTGACCCTG ATGTGCTGGT GGGTCACGAC ATCTTTGGTT TCGATTTAGA AGTGATTTTG 
CAGAGAATCA GCGTTTGCAA AGTCCCCCAC TGGTCTAAAA TTGGACGTCT CCGTCGGGCC 
AATATGCCCA AATTAGGGGG TCGCAGCGCC TTCGCAGAGA AGAGCGCCAC CTGTGGCCGC 
CTGGTGTGTG ATGTGGAGAT CTCAGCCAAG GAGCTAATAC GCTGTAAAAG TTACCACCTG 
ACTGAACTGA CCGCACACAT CCTGAAGGTG GAGAGAGTCA CGGTTCCTCA AGAAAACATC 
AGAAATCTTT ACAGCAACTC CCCTCACCTG CTTCGCCTGC TGGAGTTGAC GTGGACGGAC 
GCCAAGTTGA TCCTGCAGAT GATGTGTGAA CTGAATGTGT TGCCTCTGGC CCTGCAGATC 
ACCAACATCG CTGGCAACGT CATGTCCCGG ACTCTGATGG GCGGTCGCTC TGAGAGGAAC 
GAGTTTCTCC TGCTTCACGC CTTCCATGAG AAAAACTACA TTGTTCCTGA TAAACCCTCC 
TTCAAAAAGA CCCAGCTGGA AATGGCTGAA GGAGAAGACG AAGGTGCTGT GGGGAAAGGC 
AAGCGGAAGA AAAAGGCCGC CTATGCTGGA GGTTTGGTGC TGGATCCCAA AGTTGGTTTT 
TATGACAAGT TTGTGCTGTT GCTTGACTTC AACAGCCTGT ACCCCTCAAT CATCCAGGAG 
TTCAATATCT GCTTCACCAC TGTGCAGAGA GAGGCTCTCA ACTTGCAGAG AAAGAATGAG 
GAAGAGGATC AAGAAGAGAT TCCCGAGATT CCAGATTCAA GCCTGGAGAT GGGAATTCTC 
CCCAAAGAGA TTAGGAAGCT GGTGGAGCGA CGTAAACAAG TCAAACAGCT GATGAAACAG 
CAGGACATCA ACCCAGACGC CTACCTGCAG TATGACATCC GGCAGAAAGC TCTGAAGTTG 
ACTGCTAACA GCATGTACGG ATGTTTGGGC TTCAGCTATA GCCGCTTCTA CGCCAAGCCA 
CTCGCCGCCC TCGTCACTCA CAAAGGCAGA GAGATTTTGA TGCACACTAA AGAGATGGTT 
CAAAAGATGA ATCTGGATGT CATCTATGGC GATACAGACT CCATTATGAT AAACACCAAC 
AGCAAATCTT TGGAAGAGGT CTTCAAACTT GGCAATAAGG TGAAGGCTGA AGTAAACAAG 
CTCTACAAGC TCCTGGAGAT CGACATTGAC GGCGTGTTTA AGTCACTTCT TCTGCTGAAG 
AAGAAGAAGT ACGCAGCTTT GGTTGTGGAA AACCATGGCG ATGGACGCTA CACCTTGAAA 
CAGGAGCTCA AAGGTTTGGA CATCGTGCGC AGAGATTGGT GCGACCTGGC CAAAGAATGT 
GGCAACTATG TGATTGGTCA GATACTGTCG GATCAGAGCC GTGACGTGAT TGTGGAGAAT 
ATCCAGAAAC ATCTGGTGGA AATGGGGGAG AAGGTAGCAG CTGGAGACAT ACCACTAAAC 
CAGTATGAGA TACACAAGGC TCTGGCTAAA GATCCTCAGG ACTATCCAGA CAAGAAAAGC 
CTCCCTCATG TCCACGTGGC ACTGTGGATC AACTCTCAGG GTGGTCGCCG CGTCAAAGCT 
GGAGATACGG TCTCTTACAT CATTTGTAAG GACGGCTCCA CGCTGGCAGC CAGTCAGCGA 
GCTTACACAC AAGAGCAGCT CCAGAAACAG GAGGGTCTCA GTCTGGACAC TCAGTACTAC 
CTTGCCCAGC AGGTCCACCC TGTGGTGTCC CGAATCTGTG ACCCCATCGA GGGCATCGAT 
GGAGTGCTCA TAGCTACATG GCTGGGTCTG GATCCCAGTC AGTTCCGGGC TCAGCAGCAG 
TACCAGAGGG AGGACGAGAA TCACCTGCTG GGAGTTTCTC TCCAGCTGAC CGACGAGGAA 
CGTTACAAGG ACTGCGAGAA TTTCACCTTC ACCTGCCCAC AGTGCGGCAC AGACAACATC 
TGTGACAGTG TTTTTGAAGG AACCGGATTA AACCTGGAGC CCAGCTTGAT GCGATGCTGC 
CACATCCCCT GTGAGAGTCG GCCCATTGAC TACTCTGTCA ACATCTGCAA CAAACTGCTG 
CTCGACATCC GTCGACACAT CAAAAAATAC TACGCTGGCT GGTTGGTGTG TGAGGACCAG 
GCCTGCCAGA ACAGGACCAG ACGTTTGCCC ATTGCCTTCT CTCGCCAGGG ACCCATCTGC 
CCCGCCTGCT CCAGAGCCAC CCTCAGACCT GAGTATTCTG AGAAAGCTCT GTACAACCAG 
CTCTGCTTCT ATCGCTTCAT CTTCGACTGG GAGGCTGCAT TTTCCAAAAT CCAGGCTGAT 
GAGAAGAGTC ACGTGAAGAA CTGTAACAAA GAAAAAGAGG TGTACCGCCG GCTGAAAGAG 
GTGCCCGACA AAGCTTTAGC AGCCAGCAGC TACTCCGACA TTAACCTGGC CAAACTCTTC 
CAAGCGTTTA CCTCCCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola1 ( XM_003967875.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003967924.1 Takifugu rubripes DNA polymerase alpha 1, catalytic subunit (pola1), mRNA.

Price & Availability↑

CloneID	OTo43915
Clone ID Related Accession (Same CDS sequence)	XM_003967875.3
Accession Version	XM_003967875.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003967875.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCGG TGTCACACCC AGACAAAGAC GTGGACGCTC CGGATGGCGA TGATGGAGGC 
GATACCTGCG GCCTGGCAAA GTCTCGCTCG AGACGGGAGA AGAGAGCAAA GTCCGGGAGA 
ATGTCGGCCC TAGATCAGCT CAAGAAAGCC AAGAAGGGAG AGAAAATCAA ATATAAGGTG 
GAGGAGTTGA CAAGTGTGTA TGAGGAGGTG GATGAAGAAC AGTATTCAAA GCTTGTTCGA 
GACCGACAAG AAGACGACTG GATTGTTGAT GATGATGGAA TAGGCTATGT TGAGGATGGA 
AGGGAGATCT TTGACGATGA CCTGGAAGAA GACATTGTGG AAAACAAAGG GAGAGCTGGT 
GCTAAAGGAG CAGACGCAAA GAAAAACATG AAGAAATGTA TTGTTGCCAA GCCGAACACC 
ATTAAGAGCC TGTTCATGAA CAGCAATGTA AAAAGACCTG CTGAGAAAGA TGTGGATTTA 
TCCAAAGATG AGCTGTTAGG AGATATTTTG CAAGACCTGC ACTCTGAGAA AAAAACTCTT 
CTGACTCCTC CACCAGTTGT CACTCTTAAG AAAAAGAAGT CTCATGGATC CCCCATGAAC 
CCCTTCTCCA TAAAACCTCA GATGTCAAAG GAGTCTATGG GGTCAAAATC CAAGGTCATC 
AAGCCACCAC CAGCTAATAG CACAGCCAGA CCGTCCATCC GTCACCCCTC CTCCATCCCG 
GCTCCTCCAA CAGAGAGACA CAAGACAAAA GAGGAGGAGC TAGAAACTGA AGTGAGTCAC 
ACACTGGACT TTGACGAGGT CGACTTCGAT GAGCTGATGG AGGTCGAGCT CGAGGAGGAG 
AAACCTGTGG TAAAGGACGA AGCTAAAAGT AAAGGAGAAG CAAAGGTGGC GCCTAAAGTG 
GAGCCTAAAA TGGAACCCCA TGATCCTATT CTATTCTCCA GCAAGACTGA AAGTTCGTGG 
GAACAAGAGG AAGAAGACAG GGCCAGCGCG GTTCCAGTGG ATATCCAAGT GGACACCAGC 
CAGCTCCCCC TGGTGGAGGA GCCAGATGGG GAGCAGGTGT TCCGCTTCTA TTGGTTGGAT 
GCCTTCGAAG AGCCATACAG CCATCCAGGT GTGGTGTACC TGTTTGGAAA AGTGTGGATC 
GAGTCAGCCA AGTCTCACGT CAGCTGCTGT GTTACAGTCA GAAACATTGA GAGGACCATG 
TATCTCCTGC CTCGGGAGTA TAGAGTAAAC CCCAAAACTG GGGAGGTGTC TGACACGCCT 
GTAGGAATGA TGGATGTCTA CCAGGAGTTT AATGAGCTTT CCGAGAAGTT CAAGATCATG 
AAATACAAGT CAAAGAAAGT GGAGAAGAAC TACGCATTTG AGATTCCAGA CGTTTCCAGT 
CACGGCGAGT ACCTGGAAGT CAGATATTCT GCTGAGTTTC CTGCACTACC TTCAGACCTG 
AAGGCCTCAA CATTTTCTCA CATATTTGGG ACCAACACCT CCTTTCTGGA GCACCTGCTG 
CTCAGCAGGA AAATTAAAGG CCCGTGTTGG CTCGACGTCA CGACGCCACA GCGCATGAGT 
CAGCCCGTCA GCTGGTGTAA GGTGGAGGCT CTGGCCCTGA AGCCTGACTT GATCAGTGTG 
ATCAAAGACC TGGTGCCCCC ACCCCTCACC GTCATGTCCA TCAGCCTCAA GACGGTGCAG 
AACCCCAAAA CGCTCCACAA CGAGATCGTG TCCTTGGCAG CTCTCATCCA CCATCAGTTC 
CAGATGGACA AAGCTGCGCC CGAGCCTCCA TACCAGTCAC ATTTTTGCGT CATCAGTAAA 
CCATCAGACT GTATCTTCCC ATATGATTTC AAAGAGGCCG TGAGGAAGAA GAACGGAAAA 
GTGGAAATAG CCCAGACAGA GCGAACTCTG CTCGGCTTCT TCTTGGCAAA GATGCACAAA 
ATTGACCCTG ATGTGCTGGT GGGTCACGAC ATCTTTGGTT TCGATTTAGA AGTGATTTTG 
CAGAGAATCA GCGTTTGCAA AGTCCCCCAC TGGTCTAAAA TTGGACGTCT CCGTCGGGCC 
AATATGCCCA AATTAGGGGG TCGCAGCGCC TTCGCAGAGA AGAGCGCCAC CTGTGGCCGC 
CTGGTGTGTG ATGTGGAGAT CTCAGCCAAG GAGCTAATAC GCTGTAAAAG TTACCACCTG 
ACTGAACTGA CCGCACACAT CCTGAAGGTG GAGAGAGTCA CGGTTCCTCA AGAAAACATC 
AGAAATCTTT ACAGCAACTC CCCTCACCTG CTTCGCCTGC TGGAGTTGAC GTGGACGGAC 
GCCAAGTTGA TCCTGCAGAT GATGTGTGAA CTGAATGTGT TGCCTCTGGC CCTGCAGATC 
ACCAACATCG CTGGCAACGT CATGTCCCGG ACTCTGATGG GCGGTCGCTC TGAGAGGAAC 
GAGTTTCTCC TGCTTCACGC CTTCCATGAG AAAAACTACA TTGTTCCTGA TAAACCCTCC 
TTCAAAAAGA CCCAGCTGGA AATGGCTGAA GGAGAAGACG AAGGTGCTGT GGGGAAAGGC 
AAGCGGAAGA AAAAGGCCGC CTATGCTGGA GGTTTGGTGC TGGATCCCAA AGTTGGTTTT 
TATGACAAGT TTGTGCTGTT GCTTGACTTC AACAGCCTGT ACCCCTCAAT CATCCAGGAG 
TTCAATATCT GCTTCACCAC TGTGCAGAGA GAGGCTCTCA ACTTGCAGAG AAAGAATGAG 
GAAGAGGATC AAGAAGAGAT TCCCGAGATT CCAGATTCAA GCCTGGAGAT GGGAATTCTC 
CCCAAAGAGA TTAGGAAGCT GGTGGAGCGA CGTAAACAAG TCAAACAGCT GATGAAACAG 
CAGGACATCA ACCCAGACGC CTACCTGCAG TATGACATCC GGCAGAAAGC TCTGAAGTTG 
ACTGCTAACA GCATGTACGG ATGTTTGGGC TTCAGCTATA GCCGCTTCTA CGCCAAGCCA 
CTCGCCGCCC TCGTCACTCA CAAAGGCAGA GAGATTTTGA TGCACACTAA AGAGATGGTT 
CAAAAGATGA ATCTGGATGT CATCTATGGC GATACAGACT CCATTATGAT AAACACCAAC 
AGCAAATCTT TGGAAGAGGT CTTCAAACTT GGCAATAAGG TGAAGGCTGA AGTAAACAAG 
CTCTACAAGC TCCTGGAGAT CGACATTGAC GGCGTGTTTA AGTCACTTCT TCTGCTGAAG 
AAGAAGAAGT ACGCAGCTTT GGTTGTGGAA AACCATGGCG ATGGACGCTA CACCTTGAAA 
CAGGAGCTCA AAGGTTTGGA CATCGTGCGC AGAGATTGGT GCGACCTGGC CAAAGAATGT 
GGCAACTATG TGATTGGTCA GATACTGTCG GATCAGAGCC GTGACGTGAT TGTGGAGAAT 
ATCCAGAAAC ATCTGGTGGA AATGGGGGAG AAGGTAGCAG CTGGAGACAT ACCACTAAAC 
CAGTATGAGA TACACAAGGC TCTGGCTAAA GATCCTCAGG ACTATCCAGA CAAGAAAAGC 
CTCCCTCATG TCCACGTGGC ACTGTGGATC AACTCTCAGG GTGGTCGCCG CGTCAAAGCT 
GGAGATACGG TCTCTTACAT CATTTGTAAG GACGGCTCCA CGCTGGCAGC CAGTCAGCGA 
GCTTACACAC AAGAGCAGCT CCAGAAACAG GAGGGTCTCA GTCTGGACAC TCAGTACTAC 
CTTGCCCAGC AGGTCCACCC TGTGGTGTCC CGAATCTGTG ACCCCATCGA GGGCATCGAT 
GGAGTGCTCA TAGCTACATG GCTGGGTCTG GATCCCAGTC AGTTCCGGGC TCAGCAGCAG 
TACCAGAGGG AGGACGAGAA TCACCTGCTG GGAGTTTCTC TCCAGCTGAC CGACGAGGAA 
CGTTACAAGG ACTGCGAGAA TTTCACCTTC ACCTGCCCAC AGTGCGGCAC AGACAACATC 
TGTGACAGTG TTTTTGAAGG AACCGGATTA AACCTGGAGC CCAGCTTGAT GCGATGCTGC 
CACATCCCCT GTGAGAGTCG GCCCATTGAC TACTCTGTCA ACATCTGCAA CAAACTGCTG 
CTCGACATCC GTCGACACAT CAAAAAATAC TACGCTGGCT GGTTGGTGTG TGAGGACCAG 
GCCTGCCAGA ACAGGACCAG ACGTTTGCCC ATTGCCTTCT CTCGCCAGGG ACCCATCTGC 
CCCGCCTGCT CCAGAGCCAC CCTCAGACCT GAGTATTCTG AGAAAGCTCT GTACAACCAG 
CTCTGCTTCT ATCGCTTCAT CTTCGACTGG GAGGCTGCAT TTTCCAAAAT CCAGGCTGAT 
GAGAAGAGTC ACGTGAAGAA CTGTAACAAA GAAAAAGAGG TGTACCGCCG GCTGAAAGAG 
GTGCCCGACA AAGCTTTAGC AGCCAGCAGC TACTCCGACA TTAACCTGGC CAAACTCTTC 
CAAGCGTTTA CCTCCCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003967924.1
CDS	129..4532
Translation	MAPVSHPDKDVDAPDGDDGGDTCGLAKSRSRREKRAKSGRMSALDQLKKAKKGEKIKYKVEELTSVYEEVDEEQYSKLVRDRQEDDWIVDDDGIGYVEDGREIFDDDLEEDIVENKGRAGAKGADAKKNMKKCIVAKPNTIKSLFMNSNVKRPAEKDVDLSKDELLGDILQDLHSEKKTLLTPPPVVTLKKKKSHGSPMNPFSIKPQMSKESMGSKSKVIKPPPANSTARPSIRHPSSIPAPPTERHKTKEEELETEVSHTLDFDEVDFDELMEVELEEEKPVVKDEAKSKGEAKVAPKVEPKMEPHDPILFSSKTESSWEQEEEDRASAVPVDIQVDTSQLPLVEEPDGEQVFRFYWLDAFEEPYSHPGVVYLFGKVWIESAKSHVSCCVTVRNIERTMYLLPREYRVNPKTGEVSDTPVGMMDVYQEFNELSEKFKIMKYKSKKVEKNYAFEIPDVSSHGEYLEVRYSAEFPALPSDLKASTFSHIFGTNTSFLEHLLLSRKIKGPCWLDVTTPQRMSQPVSWCKVEALALKPDLISVIKDLVPPPLTVMSISLKTVQNPKTLHNEIVSLAALIHHQFQMDKAAPEPPYQSHFCVISKPSDCIFPYDFKEAVRKKNGKVEIAQTERTLLGFFLAKMHKIDPDVLVGHDIFGFDLEVILQRISVCKVPHWSKIGRLRRANMPKLGGRSAFAEKSATCGRLVCDVEISAKELIRCKSYHLTELTAHILKVERVTVPQENIRNLYSNSPHLLRLLELTWTDAKLILQMMCELNVLPLALQITNIAGNVMSRTLMGGRSERNEFLLLHAFHEKNYIVPDKPSFKKTQLEMAEGEDEGAVGKGKRKKKAAYAGGLVLDPKVGFYDKFVLLLDFNSLYPSIIQEFNICFTTVQREALNLQRKNEEEDQEEIPEIPDSSLEMGILPKEIRKLVERRKQVKQLMKQQDINPDAYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHKGREILMHTKEMVQKMNLDVIYGDTDSIMINTNSKSLEEVFKLGNKVKAEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALVVENHGDGRYTLKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQSRDVIVENIQKHLVEMGEKVAAGDIPLNQYEIHKALAKDPQDYPDKKSLPHVHVALWINSQGGRRVKAGDTVSYIICKDGSTLAASQRAYTQEQLQKQEGLSLDTQYYLAQQVHPVVSRICDPIEGIDGVLIATWLGLDPSQFRAQQQYQREDENHLLGVSLQLTDEERYKDCENFTFTCPQCGTDNICDSVFEGTGLNLEPSLMRCCHIPCESRPIDYSVNICNKLLLDIRRHIKKYYAGWLVCEDQACQNRTRRLPIAFSRQGPICPACSRATLRPEYSEKALYNQLCFYRFIFDWEAAFSKIQADEKSHVKNCNKEKEVYRRLKEVPDKALAASSYSDINLAKLFQAFTSLK

Target ORF information:

RefSeq Version	XM_003967875.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DNA polymerase alpha 1, catalytic subunit (pola1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003967875.3

ORF Insert Sequence:

ATGGCTCCGG TGTCACACCC AGACAAAGAC GTGGACGCTC CGGATGGCGA TGATGGAGGC 
GATACCTGCG GCCTGGCAAA GTCTCGCTCG AGACGGGAGA AGAGAGCAAA GTCCGGGAGA 
ATGTCGGCCC TAGATCAGCT CAAGAAAGCC AAGAAGGGAG AGAAAATCAA ATATAAGGTG 
GAGGAGTTGA CAAGTGTGTA TGAGGAGGTG GATGAAGAAC AGTATTCAAA GCTTGTTCGA 
GACCGACAAG AAGACGACTG GATTGTTGAT GATGATGGAA TAGGCTATGT TGAGGATGGA 
AGGGAGATCT TTGACGATGA CCTGGAAGAA GACATTGTGG AAAACAAAGG GAGAGCTGGT 
GCTAAAGGAG CAGACGCAAA GAAAAACATG AAGAAATGTA TTGTTGCCAA GCCGAACACC 
ATTAAGAGCC TGTTCATGAA CAGCAATGTA AAAAGACCTG CTGAGAAAGA TGTGGATTTA 
TCCAAAGATG AGCTGTTAGG AGATATTTTG CAAGACCTGC ACTCTGAGAA AAAAACTCTT 
CTGACTCCTC CACCAGTTGT CACTCTTAAG AAAAAGAAGT CTCATGGATC CCCCATGAAC 
CCCTTCTCCA TAAAACCTCA GATGTCAAAG GAGTCTATGG GGTCAAAATC CAAGGTCATC 
AAGCCACCAC CAGCTAATAG CACAGCCAGA CCGTCCATCC GTCACCCCTC CTCCATCCCG 
GCTCCTCCAA CAGAGAGACA CAAGACAAAA GAGGAGGAGC TAGAAACTGA AGTGAGTCAC 
ACACTGGACT TTGACGAGGT CGACTTCGAT GAGCTGATGG AGGTCGAGCT CGAGGAGGAG 
AAACCTGTGG TAAAGGACGA AGCTAAAAGT AAAGGAGAAG CAAAGGTGGC GCCTAAAGTG 
GAGCCTAAAA TGGAACCCCA TGATCCTATT CTATTCTCCA GCAAGACTGA AAGTTCGTGG 
GAACAAGAGG AAGAAGACAG GGCCAGCGCG GTTCCAGTGG ATATCCAAGT GGACACCAGC 
CAGCTCCCCC TGGTGGAGGA GCCAGATGGG GAGCAGGTGT TCCGCTTCTA TTGGTTGGAT 
GCCTTCGAAG AGCCATACAG CCATCCAGGT GTGGTGTACC TGTTTGGAAA AGTGTGGATC 
GAGTCAGCCA AGTCTCACGT CAGCTGCTGT GTTACAGTCA GAAACATTGA GAGGACCATG 
TATCTCCTGC CTCGGGAGTA TAGAGTAAAC CCCAAAACTG GGGAGGTGTC TGACACGCCT 
GTAGGAATGA TGGATGTCTA CCAGGAGTTT AATGAGCTTT CCGAGAAGTT CAAGATCATG 
AAATACAAGT CAAAGAAAGT GGAGAAGAAC TACGCATTTG AGATTCCAGA CGTTTCCAGT 
CACGGCGAGT ACCTGGAAGT CAGATATTCT GCTGAGTTTC CTGCACTACC TTCAGACCTG 
AAGGCCTCAA CATTTTCTCA CATATTTGGG ACCAACACCT CCTTTCTGGA GCACCTGCTG 
CTCAGCAGGA AAATTAAAGG CCCGTGTTGG CTCGACGTCA CGACGCCACA GCGCATGAGT 
CAGCCCGTCA GCTGGTGTAA GGTGGAGGCT CTGGCCCTGA AGCCTGACTT GATCAGTGTG 
ATCAAAGACC TGGTGCCCCC ACCCCTCACC GTCATGTCCA TCAGCCTCAA GACGGTGCAG 
AACCCCAAAA CGCTCCACAA CGAGATCGTG TCCTTGGCAG CTCTCATCCA CCATCAGTTC 
CAGATGGACA AAGCTGCGCC CGAGCCTCCA TACCAGTCAC ATTTTTGCGT CATCAGTAAA 
CCATCAGACT GTATCTTCCC ATATGATTTC AAAGAGGCCG TGAGGAAGAA GAACGGAAAA 
GTGGAAATAG CCCAGACAGA GCGAACTCTG CTCGGCTTCT TCTTGGCAAA GATGCACAAA 
ATTGACCCTG ATGTGCTGGT GGGTCACGAC ATCTTTGGTT TCGATTTAGA AGTGATTTTG 
CAGAGAATCA GCGTTTGCAA AGTCCCCCAC TGGTCTAAAA TTGGACGTCT CCGTCGGGCC 
AATATGCCCA AATTAGGGGG TCGCAGCGCC TTCGCAGAGA AGAGCGCCAC CTGTGGCCGC 
CTGGTGTGTG ATGTGGAGAT CTCAGCCAAG GAGCTAATAC GCTGTAAAAG TTACCACCTG 
ACTGAACTGA CCGCACACAT CCTGAAGGTG GAGAGAGTCA CGGTTCCTCA AGAAAACATC 
AGAAATCTTT ACAGCAACTC CCCTCACCTG CTTCGCCTGC TGGAGTTGAC GTGGACGGAC 
GCCAAGTTGA TCCTGCAGAT GATGTGTGAA CTGAATGTGT TGCCTCTGGC CCTGCAGATC 
ACCAACATCG CTGGCAACGT CATGTCCCGG ACTCTGATGG GCGGTCGCTC TGAGAGGAAC 
GAGTTTCTCC TGCTTCACGC CTTCCATGAG AAAAACTACA TTGTTCCTGA TAAACCCTCC 
TTCAAAAAGA CCCAGCTGGA AATGGCTGAA GGAGAAGACG AAGGTGCTGT GGGGAAAGGC 
AAGCGGAAGA AAAAGGCCGC CTATGCTGGA GGTTTGGTGC TGGATCCCAA AGTTGGTTTT 
TATGACAAGT TTGTGCTGTT GCTTGACTTC AACAGCCTGT ACCCCTCAAT CATCCAGGAG 
TTCAATATCT GCTTCACCAC TGTGCAGAGA GAGGCTCTCA ACTTGCAGAG AAAGAATGAG 
GAAGAGGATC AAGAAGAGAT TCCCGAGATT CCAGATTCAA GCCTGGAGAT GGGAATTCTC 
CCCAAAGAGA TTAGGAAGCT GGTGGAGCGA CGTAAACAAG TCAAACAGCT GATGAAACAG 
CAGGACATCA ACCCAGACGC CTACCTGCAG TATGACATCC GGCAGAAAGC TCTGAAGTTG 
ACTGCTAACA GCATGTACGG ATGTTTGGGC TTCAGCTATA GCCGCTTCTA CGCCAAGCCA 
CTCGCCGCCC TCGTCACTCA CAAAGGCAGA GAGATTTTGA TGCACACTAA AGAGATGGTT 
CAAAAGATGA ATCTGGATGT CATCTATGGC GATACAGACT CCATTATGAT AAACACCAAC 
AGCAAATCTT TGGAAGAGGT CTTCAAACTT GGCAATAAGG TGAAGGCTGA AGTAAACAAG 
CTCTACAAGC TCCTGGAGAT CGACATTGAC GGCGTGTTTA AGTCACTTCT TCTGCTGAAG 
AAGAAGAAGT ACGCAGCTTT GGTTGTGGAA AACCATGGCG ATGGACGCTA CACCTTGAAA 
CAGGAGCTCA AAGGTTTGGA CATCGTGCGC AGAGATTGGT GCGACCTGGC CAAAGAATGT 
GGCAACTATG TGATTGGTCA GATACTGTCG GATCAGAGCC GTGACGTGAT TGTGGAGAAT 
ATCCAGAAAC ATCTGGTGGA AATGGGGGAG AAGGTAGCAG CTGGAGACAT ACCACTAAAC 
CAGTATGAGA TACACAAGGC TCTGGCTAAA GATCCTCAGG ACTATCCAGA CAAGAAAAGC 
CTCCCTCATG TCCACGTGGC ACTGTGGATC AACTCTCAGG GTGGTCGCCG CGTCAAAGCT 
GGAGATACGG TCTCTTACAT CATTTGTAAG GACGGCTCCA CGCTGGCAGC CAGTCAGCGA 
GCTTACACAC AAGAGCAGCT CCAGAAACAG GAGGGTCTCA GTCTGGACAC TCAGTACTAC 
CTTGCCCAGC AGGTCCACCC TGTGGTGTCC CGAATCTGTG ACCCCATCGA GGGCATCGAT 
GGAGTGCTCA TAGCTACATG GCTGGGTCTG GATCCCAGTC AGTTCCGGGC TCAGCAGCAG 
TACCAGAGGG AGGACGAGAA TCACCTGCTG GGAGTTTCTC TCCAGCTGAC CGACGAGGAA 
CGTTACAAGG ACTGCGAGAA TTTCACCTTC ACCTGCCCAC AGTGCGGCAC AGACAACATC 
TGTGACAGTG TTTTTGAAGG AACCGGATTA AACCTGGAGC CCAGCTTGAT GCGATGCTGC 
CACATCCCCT GTGAGAGTCG GCCCATTGAC TACTCTGTCA ACATCTGCAA CAAACTGCTG 
CTCGACATCC GTCGACACAT CAAAAAATAC TACGCTGGCT GGTTGGTGTG TGAGGACCAG 
GCCTGCCAGA ACAGGACCAG ACGTTTGCCC ATTGCCTTCT CTCGCCAGGG ACCCATCTGC 
CCCGCCTGCT CCAGAGCCAC CCTCAGACCT GAGTATTCTG AGAAAGCTCT GTACAACCAG 
CTCTGCTTCT ATCGCTTCAT CTTCGACTGG GAGGCTGCAT TTTCCAAAAT CCAGGCTGAT 
GAGAAGAGTC ACGTGAAGAA CTGTAACAAA GAAAAAGAGG TGTACCGCCG GCTGAAAGAG 
GTGCCCGACA AAGCTTTAGC AGCCAGCAGC TACTCCGACA TTAACCTGGC CAAACTCTTC 
CAAGCGTTTA CCTCCCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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pola1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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