cacna1d cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1d
Entrez Gene ID	101066777
Full Name	calcium voltage-gated channel subunit alpha1 D
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029833248.1	XP_029689108.1	voltage-dependent L-type calcium channel subunit alpha-1D
XM_011621847.1	XP_011620149.1	voltage-dependent L-type calcium channel subunit alpha-1D

Pathways from BioSystems
Gene Ontology

The following cacna1d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo22781	XM_011621847.1 Latest version!	Takifugu rubripes calcium channel, voltage-dependent, L type, alpha 1D subunit (cacna1d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo63162	XM_029833248.1 Latest version!	Takifugu rubripes calcium voltage-gated channel subunit alpha1 D (cacna1d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1d ( XM_011621847.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011620149.1 Takifugu rubripes calcium channel, voltage-dependent, L type, alpha 1D subunit (cacna1d), mRNA.

Price & Availability↑

CloneID	OTo22781
Clone ID Related Accession (Same CDS sequence)	XM_011621847.1
Accession Version	XM_011621847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	voltage-dependent L-type calcium channel subunit alpha-1D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070929.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 10% of CDS bases ##RefSeq-Attributes-END##

ATGCAAGAGG CAGATGAACA GACTGAAGGG GCACAGTATG CCGGCAGCAG AAGGACCACT 
GTCTCCACCA ACGCCTCAGC ACTCTGGGCA GAAAACCAAA CCTCTTTGCA CCAGCTCCAC 
CCGTCAGAGC ACCCACAGGC TCCAGCTAGC ACCGGCAAGC CTGCGGGCCC CTACCCTGGA 
CTTCTCGGGA CCAACAGTGG CATTCCGACC CACGGAGGTG CCGGTTCAGG CGTCAACCCT 
GTCCTCGCTT GGCATGCCGC CATCAACGCC GCACGCCAAG CGCAAGGCGA CGGTGCAAAG 
CATGACGTGA GCTCGCCGCC ACCTGTCTGC ACCACTGGAC CACCTCCCGT GGGCTCGCTG 
GCGCAGAAGA AGAGGCAGCA GTACGCCAAA AGCAAAAAGC AGGGAGGCTC TACCAACAGC 
AGGCCACCGA GAGCCCTGTT CTGCCTCACC CTCAACAACC CCATCCGCAG GGCCTGCATC 
AGCCTGGTGG AGTGGAAACC ATTTGATATC TTTATACTTC TGTCTATTTT TGCCAACTGC 
GTGGCCTTAG CCATTTACAT CCCCTTTCCT GGAGACGACT CCAACTCCAC CAACCAAGAA 
CTGGAAACAG TGGAATATGC CTTCCTGATC ATTTTCACTA TAGAGACGTT TCTGAAGATT 
ATTGCCTACG GCCTGGTCAT GCACCAAAAC TCTTACGTCC GAAACGGCTG GAACTTGCTG 
GATTTTGTGA TCGTCATCGT TGGGTTGTTC AGTGTGGCTC TGGAGATGAT CACCAAAGAC 
GCCGACTCCA GCGGACAGTC TGGAGGCAAA CCCGGGGGGT TCGATGTCAA GGCCCTCCGA 
GCCTTTCGTG TCCTTCGTCC CCTTCGCCTT GTCTCGGGAG TTCCCAGTTT ACAGGTGGTT 
TTAAACTCCA TTATTAAAGC CATGGTTCCC CTGCTTCACA TCGCGCTGCT GGTACTCTTC 
GTCATCATCA TCTACGCCGT CATCGGCCTG GAGCTTTTCA TTGGCAAAAT GCACGCCACG 
TGCTACGTGA TAGGAACAGG TAACCTTGCA GAAGAGGAGC CCACACCGTG TGCTGTGTCC 
GGGCATGGCC GCCACTGCCT GCTCAACGGC ACGCTTTGCA GGGAGGGCTG GCAAGGTCCC 
AACAATGGCA TCACCAACTT CGACAATTTT TTGTTTGCCA TGCTGACTGT GTTCCAGTGC 
ATCACTATGG AGGGCTGGAC GGATGTTCTT TACTGGATGA ATGATGCGAT GGGCTTCGAG 
TTGCCGTGGG TCTACTTTGT CAGCCTGGTC ATCTTTGGAT CCTTCTTTGT TCTCAACCTG 
GTTTTGGGCG TGTTGAGCGG AGAGTTCTCC AAAGAGCGCG AGAAGGCCAA AGCTCGGGGA 
GATTTCCAAA AGCTACGCGA GAAGCAGCAG TTAGAGGAAG ACCTGAAGGG TTACCTGGAC 
TGGATCACCC AGGCTGAGGA CATCGACCCC GAGAATGAGG AGGAGGGAGA GGAAGATGGC 
AAACGCAATC GGGTCACTCT GGCTGACCTC ACTGAGAAGA AGAAAGGAAA GTTTGGCTGG 
TTCACCCAGT CCACAGAGAC ACAGGCGAGC ATGCCGAGCG AGAACGAGTC GGCGAACACG 
GACAACCACG CTGGCGCGGA TGCCAAATCG CCCTGCTGTG GCCCGCTGTG TCAAAAAATA 
ACTAAGTCAA AATTCAGCCG CCAGTGGAGG CGCTGGAACC GCTTCTGCCG CAGGAAGTGC 
CGCGCCGCTG TGAAATCCGT CACATTTTAC TGGCTGGTCA TCATCCTGGT GTTTCTCAAC 
ACTCTCACCA TTGCCTCTGA GCACTACAAC CAGCCAGACT GGCTGACCGA AGTCCAGGAC 
GTTGCCAACA AAGTCCTCCT GGCCATGTTC ACCCTGGAGA TGCTCGTGAA GTTGTACAGC 
CTGGGGCTGC AGGCCTACTT TGTGTCGCTG TTTAATCGCT TCGACTGTTT CGTGGTGTGC 
GGCGGCATCG TGGAGACCAT CCTGGTGGAG CTCGCTATCA TGTCGCCGCT CGGTATCTCC 
GTCTTCCGCT GCGTACGACT CCTGCGGATC TTCAAGGTCA CGCGACATTG GGCGTCTCTC 
AGCAACCTGG TGGCCTCCCT GCTGAACTCC ATGAAGTCCA TCGCCTCTCT GTTGCTGCTG 
CTCTTCCTCT TCATCATCAT CTTTTCCCTG CTGGGCATGC AGCTTTTCGG GGGGAAGTTT 
AATTTTGACG AGACTGTGAC CAAAAGAAGC ACTTTTGACA ACTTCCCTCA GGCTCTGCTC 
ACTGTGTTCC AGATCCTGAC AGGAGAAGAC TGGAATACTG TGATGTACGA TGGCATCATG 
GCGTACGGCG GGCCGGCCTC CTCTGGAATG TTGGTCTCCA TTTACTTCAT CATCCTCTTC 
ATCTGTGGAA ATTACATCCT GCTAAATGTC TTCTTGGCTA TCGCTGTTGA CAACCTGGCA 
GATGCAGAAA GCCTCAACAC AGCACAGAAG GAAGAAGAAG AAGCAAAGAA GAGGAGGAAC 
AGTGCCAAGG ATAATAGAGT GGATCTAATA GAAGGCAAAG AGACCAAGAA AAACCAACAA 
AAACGTAATT TTATTTCAGT TTCTGATGAT GATGAGGAGG AGGAGAATTA TGAGGACCTC 
CCAGAAGTCC CACCGGGGCC TCGCCCTCAG AGGTTGTCTG AACTCACCAT CAAGGAGAAG 
ATTCCACCCA TCCCAGCAGG AAGCGCCTTC TTCGTCTTCA GTAGCACCAA CCCGTTCCGT 
GTGTTCTGCC ACAAACTGAT CAACCACCAG ATCTTCACCA ACCTCATCTT GGTCTTCATC 
ATGCTCAGCT CCGTGTCCCT GGCTGCCGAG GACCCCATAC GCAACTTCTC GGCCCGCAAC 
ATCATACTTG GTTATTTTGA CTATGCTTTC ACCGCAATCT TTACTGTTGA GATCCTGTTG 
AAGATGACAG CGTTCGGAGC GTTTCTCCAT CAAGGAGCAT TTTGTCGGAA CTACTTCAAC 
CTTTTGGATC TGCTGGTGGT CGGAGTGTCT CTGGTCTCCT TCGGCATTCA GTCCTCTGCT 
ATCTCAGTCG TGAAGATCCT TCGTGTTTTA AGAGTCCTTC GTCCACTCAG GGCCATCAAT 
AGAGCCAAAG GCCTCAAGCA CGTGGTCCAG TGTGTCTTCG TGGCCATCAG GACCATCGGT 
AACATCATGA TTGTCACCAC GCTGCTGCAG TTCATGTTCG CCTGTATCGG AGTGCAGCTC 
TTTAAGGGAA AATTTTATCG CTGCACCGAT GATGCAAAGT CCAGTCCAGA GGAGTGCAAA 
GGCACCTACA TTCTGTACAA CAACGGGGAT GCAGCGCTGC CTATGGCGAA GGAGAGGATC 
TGGTACAACA GCGACTTCAA CTTTGACAAT GTCCTCATGG CCATGATGGC CCTCTTTACT 
GTCTCCACCT TTGAAGGATG GCCCACGCTT CTGTACAAGG CTATTGACTC CAACAAAGAG 
AACATGGGTC CCATCTACAA CTACCGCATC GAGATCTCCA TCTTCTTCAT CATCTACATC 
ATCATCATCG CTTTCTTCAT GATGAACATC TTTGTCGGTT TTGTTATTGT CACCTTTCAG 
GAGCAGGGGG AGAAAGAATA CAAAAACTGC GAACTGGACA AGAACCAGCG TCAGTGTGTG 
GAATACGCTC TTAAAGCTCG TCCACTGCGG AGATATATCC CCAAAAACCC GTACCAGTAC 
AAGTTCTGGT ACGTGGTGAA CTCTACTGGC TTCGAGTATG TCATGTTCAT ACTTATCATC 
CTCAACACCT TGTGTCTGGC CATTCGTAAG TTGCACCATG GTCAGTCTCA TTTGTTCAAC 
TATGCCATGG ACATCCTCAA CATGGTTTTC ACTGGGGTTT TCACTGTGGA GATGATCCTC 
AAGCTCATTG CCTTCAGCTC ACAGAACTAT TTTGCTGATG CATGGAACAC ATTTGATGCC 
TTAATTGTTG TGGGTAGCGT GGTTGATATT GCCATCACTG AGATCAATGT AAGTAGTCAT 
TTTGGCACCC ACACGGAGGA CAGCGCTCGC ATCTCAATCA CCTTCTTCCG CTTATTTAGA 
GTGATGAGGC TGGTCAAGCT CCTGAGCAGA GGGGAGGGGA TCAGGACTCT TCTGTGGACC 
TTCATTAAAT CCTTCCAGGC CCTTCCCTAC GTCGCGCTCC TGATAGCCAT GCTCTTCTTC 
ATCTATGCTG TAATCGGAAT GCAGGTTTTT GGGAAAATAG CCATGGTCGA CGGAACGCAA 
ATCAATCGCA ACAACAACTT TCAAACCTTC CCTCAAGCTG TCCTGATGCT CTTCAGGTGC 
GCCACAGGTG AGGCCTGGCA GGAGATCATG CTGGCCTGCC TGCCAGGGAA GCTTTGCGAC 
TCAGAGTCAG ACTACAACCC CGGCGAGGAG CGAACCTGCG GCAGCGGCTT CGCCATCTTC 
TACTTCATCA GCTTTTACAT GCTCTGTGCT TTTCTGATCA TTAATCTATT CGTAGCCGTC 
ATCATGGATA ACTTTGACTA CCTGACGCGT GATTGGTCAA TCCTTGGACC ACATCATCTC 
GATGAGTTTA AAAGGATTTG GTCTGAATAC GACCCTGAAG CCAAGGGTCG GATAAAGCAC 
CTTGATGTGG TGACCCTGTT GAGGAGGATC CAGCCCCCGC TGGGCTTTGG CAAGCTTTGT 
CCTCACAGAG TGGCCTGTAA GCGCCTGGTG GCGATGAACA TGCCCCTCAA CAGTGACGGG 
ACAGTCATGT TTAATGCCAC CTTGTTCGCT CTGGTCCGCA CCGCTCTCAA GATTAAGACG 
GAGGGAAACT TGGATCAGGC CAATGAGGAG CTCAGAGCTG TGATCAAGAA GATCTGGAAG 
AGGACCAGCA TGAAGCTGCT GGACCAAGTT GTGCCCCCTG CCGGCGATGA CGAGGTCACT 
GTGGGGAAGT TCTATGCCAC CTTCCTGATA CAGGACTACT TCAGGAAATT CAAGAGGCGC 
AAAGAGCAAG GTCTTGTGGG TAAGCGCTCG AGGGAGAGGC TCAGCGCCAC CATGGCTTTA 
CAGGCCGGCC TCCGCACCCT GCACGACATT GGACCAGAGA TTCGCAGGGC CATATCATGT 
GACCTGCAGC AGGAGGGGCT GGTAGATGGT AATGAAGAAG AGGAAGAAGA GCAAATCTAC 
AGGCGCAACG GAGGCCTTTT TGGGAACTAC ATCAACCAAC TCAATGCAGA CAAACAAGGT 
TTCTCCAATC TGACCACCGC CACCCAGCGG CCCTTGCAGA TCCTACCCCT CTCCCACACC 
GCGGTCGAGC CCATGACCAC AGTCAGGAGA GCCAGCGGAG GGGAGAGGCA GCTCAACGCC 
TCACCCATCC ACAATCATTG TCACCACCCT CACCCCTACA ACCACCCCGC CTGCCAGCAG 
TCACCCATCC CCTCCAATGC CAACCTCAAC AACGCCAACG TGCCCTCACT TCTGGCCATG 
AACACTGGGA GGCAGCAGAG GTGTTTGTTA AGGCGCGACC GCCCCTCCTC CACTAAGAAC 
GGCTCGTCAG CGTGTACTTA CAGTAAAGGT GTCCATGAGA AACTGTGGAA GTCACACTCA 
AGCAGGTCAG AAAGCAGCCT CACCGTCTCA AGGGAGGATG TCAGAGACGA GGACAGCGAG 
GAGGAGAGAG GCTCGGGGGA GTACTACAGC GGGGAGGAGT TTAATGAAGA TGACATCATG 
CTTGCGAGGG ACAGGTTAGT GAAGCCTGAC AAACTTGACG CCGAGGACGA CGCTGCCCTG 
CAGGTTTCCG CCGACGACCA CCTCGATGTT TTCTACGATG ACGACCAGCA GCCGATATAC 
CAAACAGACA GGCGTTCGCC CAGGAGATGG TTTCTCCCCT CACCTCAAAC CTCAAAAAGG 
CCGCCATTCA ATTTTGAGTG TCTTCGCAAA AAGAGCAGTG AAGAGGAGAC CCCCCCGCCA 
CCTTCCCCAT CCTGCACAGC TCTGCCACTG CATCTGGTGC AGCAGCAGGT GATGGCGGTG 
GCCGGCCTCG ATGCCAGCAG ACTGCGCCAC CTGTCGCCAG CACGGTCGCT TTGCTCCTGG 
GCCACACCCC CCGCCTCACC CATCCACCTT GACGGCTCCC CTAACTACAC GCCCCTGATA 
CAGGTGGACT GGCACCGATC CGATAGCGTG GGCAGCATCC CCACGGCTGT GAAGAGGAGT 
TTGTGGTACA CGCACGGCCA GGAGCTCCCT CCCAGCCGCA GCCATTCTCC GTCACACCTG 
CAGCTGCCAG CTGACGGCCT CTCGCGCTTC CTGCAGAAGA GGGGCAGCGC CAACAGCTTG 
GTGGAGGCGG TGCTGATCTC CGAAGGTTTG GCAAAATACG CCAAGGACCC CAAATTCGTG 
TCCGCGACCA AGCACGAGAT CGCGGACGCC TGCGAGATGA CCATCGACGA GATGGACAGC 
GCCGCCAGCA ACCTCCTGAA CGGCGGGATG GACAACGGAA GCAGCGGGAC AACCAGCAGC 
GGTGACTCAG ACCCGGACGC GGGTCACCTC AGCGACCGCA GCGTCCGCGA CTACAGCGAC 
GAGGAGCCGA GCGCGACCGT CAAGCGGGAC GAGGACCTGA CCGACGAGAT GATCTGCATC 
ACCTCGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011620149.1
CDS	1..6612
Translation	MQEADEQTEGAQYAGSRRTTVSTNASALWAENQTSLHQLHPSEHPQAPASTGKPAGPYPGLLGTNSGIPTHGGAGSGVNPVLAWHAAINAARQAQGDGAKHDVSSPPPVCTTGPPPVGSLAQKKRQQYAKSKKQGGSTNSRPPRALFCLTLNNPIRRACISLVEWKPFDIFILLSIFANCVALAIYIPFPGDDSNSTNQELETVEYAFLIIFTIETFLKIIAYGLVMHQNSYVRNGWNLLDFVIVIVGLFSVALEMITKDADSSGQSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAVIGLELFIGKMHATCYVIGTGNLAEEEPTPCAVSGHGRHCLLNGTLCREGWQGPNNGITNFDNFLFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGEEDGKRNRVTLADLTEKKKGKFGWFTQSTETQASMPSENESANTDNHAGADAKSPCCGPLCQKITKSKFSRQWRRWNRFCRRKCRAAVKSVTFYWLVIILVFLNTLTIASEHYNQPDWLTEVQDVANKVLLAMFTLEMLVKLYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELAIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETVTKRSTFDNFPQALLTVFQILTGEDWNTVMYDGIMAYGGPASSGMLVSIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEEEAKKRRNSAKDNRVDLIEGKETKKNQQKRNFISVSDDDEEEENYEDLPEVPPGPRPQRLSELTIKEKIPPIPAGSAFFVFSSTNPFRVFCHKLINHQIFTNLILVFIMLSSVSLAAEDPIRNFSARNIILGYFDYAFTAIFTVEILLKMTAFGAFLHQGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDDAKSSPEECKGTYILYNNGDAALPMAKERIWYNSDFNFDNVLMAMMALFTVSTFEGWPTLLYKAIDSNKENMGPIYNYRIEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYVMFILIILNTLCLAIRKLHHGQSHLFNYAMDILNMVFTGVFTVEMILKLIAFSSQNYFADAWNTFDALIVVGSVVDIAITEINVSSHFGTHTEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLMLFRCATGEAWQEIMLACLPGKLCDSESDYNPGEERTCGSGFAIFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLDQANEELRAVIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKRRKEQGLVGKRSRERLSATMALQAGLRTLHDIGPEIRRAISCDLQQEGLVDGNEEEEEEQIYRRNGGLFGNYINQLNADKQGFSNLTTATQRPLQILPLSHTAVEPMTTVRRASGGERQLNASPIHNHCHHPHPYNHPACQQSPIPSNANLNNANVPSLLAMNTGRQQRCLLRRDRPSSTKNGSSACTYSKGVHEKLWKSHSSRSESSLTVSREDVRDEDSEEERGSGEYYSGEEFNEDDIMLARDRLVKPDKLDAEDDAALQVSADDHLDVFYDDDQQPIYQTDRRSPRRWFLPSPQTSKRPPFNFECLRKKSSEEETPPPPSPSCTALPLHLVQQQVMAVAGLDASRLRHLSPARSLCSWATPPASPIHLDGSPNYTPLIQVDWHRSDSVGSIPTAVKRSLWYTHGQELPPSRSHSPSHLQLPADGLSRFLQKRGSANSLVEAVLISEGLAKYAKDPKFVSATKHEIADACEMTIDEMDSAASNLLNGGMDNGSSGTTSSGDSDPDAGHLSDRSVRDYSDEEPSATVKRDEDLTDEMICITSL

Target ORF information:

RefSeq Version	XM_011621847.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes calcium channel, voltage-dependent, L type, alpha 1D subunit (cacna1d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011621847.1

ORF Insert Sequence:

ATGCAAGAGG CAGATGAACA GACTGAAGGG GCACAGTATG CCGGCAGCAG AAGGACCACT 
GTCTCCACCA ACGCCTCAGC ACTCTGGGCA GAAAACCAAA CCTCTTTGCA CCAGCTCCAC 
CCGTCAGAGC ACCCACAGGC TCCAGCTAGC ACCGGCAAGC CTGCGGGCCC CTACCCTGGA 
CTTCTCGGGA CCAACAGTGG CATTCCGACC CACGGAGGTG CCGGTTCAGG CGTCAACCCT 
GTCCTCGCTT GGCATGCCGC CATCAACGCC GCACGCCAAG CGCAAGGCGA CGGTGCAAAG 
CATGACGTGA GCTCGCCGCC ACCTGTCTGC ACCACTGGAC CACCTCCCGT GGGCTCGCTG 
GCGCAGAAGA AGAGGCAGCA GTACGCCAAA AGCAAAAAGC AGGGAGGCTC TACCAACAGC 
AGGCCACCGA GAGCCCTGTT CTGCCTCACC CTCAACAACC CCATCCGCAG GGCCTGCATC 
AGCCTGGTGG AGTGGAAACC ATTTGATATC TTTATACTTC TGTCTATTTT TGCCAACTGC 
GTGGCCTTAG CCATTTACAT CCCCTTTCCT GGAGACGACT CCAACTCCAC CAACCAAGAA 
CTGGAAACAG TGGAATATGC CTTCCTGATC ATTTTCACTA TAGAGACGTT TCTGAAGATT 
ATTGCCTACG GCCTGGTCAT GCACCAAAAC TCTTACGTCC GAAACGGCTG GAACTTGCTG 
GATTTTGTGA TCGTCATCGT TGGGTTGTTC AGTGTGGCTC TGGAGATGAT CACCAAAGAC 
GCCGACTCCA GCGGACAGTC TGGAGGCAAA CCCGGGGGGT TCGATGTCAA GGCCCTCCGA 
GCCTTTCGTG TCCTTCGTCC CCTTCGCCTT GTCTCGGGAG TTCCCAGTTT ACAGGTGGTT 
TTAAACTCCA TTATTAAAGC CATGGTTCCC CTGCTTCACA TCGCGCTGCT GGTACTCTTC 
GTCATCATCA TCTACGCCGT CATCGGCCTG GAGCTTTTCA TTGGCAAAAT GCACGCCACG 
TGCTACGTGA TAGGAACAGG TAACCTTGCA GAAGAGGAGC CCACACCGTG TGCTGTGTCC 
GGGCATGGCC GCCACTGCCT GCTCAACGGC ACGCTTTGCA GGGAGGGCTG GCAAGGTCCC 
AACAATGGCA TCACCAACTT CGACAATTTT TTGTTTGCCA TGCTGACTGT GTTCCAGTGC 
ATCACTATGG AGGGCTGGAC GGATGTTCTT TACTGGATGA ATGATGCGAT GGGCTTCGAG 
TTGCCGTGGG TCTACTTTGT CAGCCTGGTC ATCTTTGGAT CCTTCTTTGT TCTCAACCTG 
GTTTTGGGCG TGTTGAGCGG AGAGTTCTCC AAAGAGCGCG AGAAGGCCAA AGCTCGGGGA 
GATTTCCAAA AGCTACGCGA GAAGCAGCAG TTAGAGGAAG ACCTGAAGGG TTACCTGGAC 
TGGATCACCC AGGCTGAGGA CATCGACCCC GAGAATGAGG AGGAGGGAGA GGAAGATGGC 
AAACGCAATC GGGTCACTCT GGCTGACCTC ACTGAGAAGA AGAAAGGAAA GTTTGGCTGG 
TTCACCCAGT CCACAGAGAC ACAGGCGAGC ATGCCGAGCG AGAACGAGTC GGCGAACACG 
GACAACCACG CTGGCGCGGA TGCCAAATCG CCCTGCTGTG GCCCGCTGTG TCAAAAAATA 
ACTAAGTCAA AATTCAGCCG CCAGTGGAGG CGCTGGAACC GCTTCTGCCG CAGGAAGTGC 
CGCGCCGCTG TGAAATCCGT CACATTTTAC TGGCTGGTCA TCATCCTGGT GTTTCTCAAC 
ACTCTCACCA TTGCCTCTGA GCACTACAAC CAGCCAGACT GGCTGACCGA AGTCCAGGAC 
GTTGCCAACA AAGTCCTCCT GGCCATGTTC ACCCTGGAGA TGCTCGTGAA GTTGTACAGC 
CTGGGGCTGC AGGCCTACTT TGTGTCGCTG TTTAATCGCT TCGACTGTTT CGTGGTGTGC 
GGCGGCATCG TGGAGACCAT CCTGGTGGAG CTCGCTATCA TGTCGCCGCT CGGTATCTCC 
GTCTTCCGCT GCGTACGACT CCTGCGGATC TTCAAGGTCA CGCGACATTG GGCGTCTCTC 
AGCAACCTGG TGGCCTCCCT GCTGAACTCC ATGAAGTCCA TCGCCTCTCT GTTGCTGCTG 
CTCTTCCTCT TCATCATCAT CTTTTCCCTG CTGGGCATGC AGCTTTTCGG GGGGAAGTTT 
AATTTTGACG AGACTGTGAC CAAAAGAAGC ACTTTTGACA ACTTCCCTCA GGCTCTGCTC 
ACTGTGTTCC AGATCCTGAC AGGAGAAGAC TGGAATACTG TGATGTACGA TGGCATCATG 
GCGTACGGCG GGCCGGCCTC CTCTGGAATG TTGGTCTCCA TTTACTTCAT CATCCTCTTC 
ATCTGTGGAA ATTACATCCT GCTAAATGTC TTCTTGGCTA TCGCTGTTGA CAACCTGGCA 
GATGCAGAAA GCCTCAACAC AGCACAGAAG GAAGAAGAAG AAGCAAAGAA GAGGAGGAAC 
AGTGCCAAGG ATAATAGAGT GGATCTAATA GAAGGCAAAG AGACCAAGAA AAACCAACAA 
AAACGTAATT TTATTTCAGT TTCTGATGAT GATGAGGAGG AGGAGAATTA TGAGGACCTC 
CCAGAAGTCC CACCGGGGCC TCGCCCTCAG AGGTTGTCTG AACTCACCAT CAAGGAGAAG 
ATTCCACCCA TCCCAGCAGG AAGCGCCTTC TTCGTCTTCA GTAGCACCAA CCCGTTCCGT 
GTGTTCTGCC ACAAACTGAT CAACCACCAG ATCTTCACCA ACCTCATCTT GGTCTTCATC 
ATGCTCAGCT CCGTGTCCCT GGCTGCCGAG GACCCCATAC GCAACTTCTC GGCCCGCAAC 
ATCATACTTG GTTATTTTGA CTATGCTTTC ACCGCAATCT TTACTGTTGA GATCCTGTTG 
AAGATGACAG CGTTCGGAGC GTTTCTCCAT CAAGGAGCAT TTTGTCGGAA CTACTTCAAC 
CTTTTGGATC TGCTGGTGGT CGGAGTGTCT CTGGTCTCCT TCGGCATTCA GTCCTCTGCT 
ATCTCAGTCG TGAAGATCCT TCGTGTTTTA AGAGTCCTTC GTCCACTCAG GGCCATCAAT 
AGAGCCAAAG GCCTCAAGCA CGTGGTCCAG TGTGTCTTCG TGGCCATCAG GACCATCGGT 
AACATCATGA TTGTCACCAC GCTGCTGCAG TTCATGTTCG CCTGTATCGG AGTGCAGCTC 
TTTAAGGGAA AATTTTATCG CTGCACCGAT GATGCAAAGT CCAGTCCAGA GGAGTGCAAA 
GGCACCTACA TTCTGTACAA CAACGGGGAT GCAGCGCTGC CTATGGCGAA GGAGAGGATC 
TGGTACAACA GCGACTTCAA CTTTGACAAT GTCCTCATGG CCATGATGGC CCTCTTTACT 
GTCTCCACCT TTGAAGGATG GCCCACGCTT CTGTACAAGG CTATTGACTC CAACAAAGAG 
AACATGGGTC CCATCTACAA CTACCGCATC GAGATCTCCA TCTTCTTCAT CATCTACATC 
ATCATCATCG CTTTCTTCAT GATGAACATC TTTGTCGGTT TTGTTATTGT CACCTTTCAG 
GAGCAGGGGG AGAAAGAATA CAAAAACTGC GAACTGGACA AGAACCAGCG TCAGTGTGTG 
GAATACGCTC TTAAAGCTCG TCCACTGCGG AGATATATCC CCAAAAACCC GTACCAGTAC 
AAGTTCTGGT ACGTGGTGAA CTCTACTGGC TTCGAGTATG TCATGTTCAT ACTTATCATC 
CTCAACACCT TGTGTCTGGC CATTCGTAAG TTGCACCATG GTCAGTCTCA TTTGTTCAAC 
TATGCCATGG ACATCCTCAA CATGGTTTTC ACTGGGGTTT TCACTGTGGA GATGATCCTC 
AAGCTCATTG CCTTCAGCTC ACAGAACTAT TTTGCTGATG CATGGAACAC ATTTGATGCC 
TTAATTGTTG TGGGTAGCGT GGTTGATATT GCCATCACTG AGATCAATGT AAGTAGTCAT 
TTTGGCACCC ACACGGAGGA CAGCGCTCGC ATCTCAATCA CCTTCTTCCG CTTATTTAGA 
GTGATGAGGC TGGTCAAGCT CCTGAGCAGA GGGGAGGGGA TCAGGACTCT TCTGTGGACC 
TTCATTAAAT CCTTCCAGGC CCTTCCCTAC GTCGCGCTCC TGATAGCCAT GCTCTTCTTC 
ATCTATGCTG TAATCGGAAT GCAGGTTTTT GGGAAAATAG CCATGGTCGA CGGAACGCAA 
ATCAATCGCA ACAACAACTT TCAAACCTTC CCTCAAGCTG TCCTGATGCT CTTCAGGTGC 
GCCACAGGTG AGGCCTGGCA GGAGATCATG CTGGCCTGCC TGCCAGGGAA GCTTTGCGAC 
TCAGAGTCAG ACTACAACCC CGGCGAGGAG CGAACCTGCG GCAGCGGCTT CGCCATCTTC 
TACTTCATCA GCTTTTACAT GCTCTGTGCT TTTCTGATCA TTAATCTATT CGTAGCCGTC 
ATCATGGATA ACTTTGACTA CCTGACGCGT GATTGGTCAA TCCTTGGACC ACATCATCTC 
GATGAGTTTA AAAGGATTTG GTCTGAATAC GACCCTGAAG CCAAGGGTCG GATAAAGCAC 
CTTGATGTGG TGACCCTGTT GAGGAGGATC CAGCCCCCGC TGGGCTTTGG CAAGCTTTGT 
CCTCACAGAG TGGCCTGTAA GCGCCTGGTG GCGATGAACA TGCCCCTCAA CAGTGACGGG 
ACAGTCATGT TTAATGCCAC CTTGTTCGCT CTGGTCCGCA CCGCTCTCAA GATTAAGACG 
GAGGGAAACT TGGATCAGGC CAATGAGGAG CTCAGAGCTG TGATCAAGAA GATCTGGAAG 
AGGACCAGCA TGAAGCTGCT GGACCAAGTT GTGCCCCCTG CCGGCGATGA CGAGGTCACT 
GTGGGGAAGT TCTATGCCAC CTTCCTGATA CAGGACTACT TCAGGAAATT CAAGAGGCGC 
AAAGAGCAAG GTCTTGTGGG TAAGCGCTCG AGGGAGAGGC TCAGCGCCAC CATGGCTTTA 
CAGGCCGGCC TCCGCACCCT GCACGACATT GGACCAGAGA TTCGCAGGGC CATATCATGT 
GACCTGCAGC AGGAGGGGCT GGTAGATGGT AATGAAGAAG AGGAAGAAGA GCAAATCTAC 
AGGCGCAACG GAGGCCTTTT TGGGAACTAC ATCAACCAAC TCAATGCAGA CAAACAAGGT 
TTCTCCAATC TGACCACCGC CACCCAGCGG CCCTTGCAGA TCCTACCCCT CTCCCACACC 
GCGGTCGAGC CCATGACCAC AGTCAGGAGA GCCAGCGGAG GGGAGAGGCA GCTCAACGCC 
TCACCCATCC ACAATCATTG TCACCACCCT CACCCCTACA ACCACCCCGC CTGCCAGCAG 
TCACCCATCC CCTCCAATGC CAACCTCAAC AACGCCAACG TGCCCTCACT TCTGGCCATG 
AACACTGGGA GGCAGCAGAG GTGTTTGTTA AGGCGCGACC GCCCCTCCTC CACTAAGAAC 
GGCTCGTCAG CGTGTACTTA CAGTAAAGGT GTCCATGAGA AACTGTGGAA GTCACACTCA 
AGCAGGTCAG AAAGCAGCCT CACCGTCTCA AGGGAGGATG TCAGAGACGA GGACAGCGAG 
GAGGAGAGAG GCTCGGGGGA GTACTACAGC GGGGAGGAGT TTAATGAAGA TGACATCATG 
CTTGCGAGGG ACAGGTTAGT GAAGCCTGAC AAACTTGACG CCGAGGACGA CGCTGCCCTG 
CAGGTTTCCG CCGACGACCA CCTCGATGTT TTCTACGATG ACGACCAGCA GCCGATATAC 
CAAACAGACA GGCGTTCGCC CAGGAGATGG TTTCTCCCCT CACCTCAAAC CTCAAAAAGG 
CCGCCATTCA ATTTTGAGTG TCTTCGCAAA AAGAGCAGTG AAGAGGAGAC CCCCCCGCCA 
CCTTCCCCAT CCTGCACAGC TCTGCCACTG CATCTGGTGC AGCAGCAGGT GATGGCGGTG 
GCCGGCCTCG ATGCCAGCAG ACTGCGCCAC CTGTCGCCAG CACGGTCGCT TTGCTCCTGG 
GCCACACCCC CCGCCTCACC CATCCACCTT GACGGCTCCC CTAACTACAC GCCCCTGATA 
CAGGTGGACT GGCACCGATC CGATAGCGTG GGCAGCATCC CCACGGCTGT GAAGAGGAGT 
TTGTGGTACA CGCACGGCCA GGAGCTCCCT CCCAGCCGCA GCCATTCTCC GTCACACCTG 
CAGCTGCCAG CTGACGGCCT CTCGCGCTTC CTGCAGAAGA GGGGCAGCGC CAACAGCTTG 
GTGGAGGCGG TGCTGATCTC CGAAGGTTTG GCAAAATACG CCAAGGACCC CAAATTCGTG 
TCCGCGACCA AGCACGAGAT CGCGGACGCC TGCGAGATGA CCATCGACGA GATGGACAGC 
GCCGCCAGCA ACCTCCTGAA CGGCGGGATG GACAACGGAA GCAGCGGGAC AACCAGCAGC 
GGTGACTCAG ACCCGGACGC GGGTCACCTC AGCGACCGCA GCGTCCGCGA CTACAGCGAC 
GAGGAGCCGA GCGCGACCGT CAAGCGGGAC GAGGACCTGA CCGACGAGAT GATCTGCATC 
ACCTCGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1d ( XM_029833248.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029689108.1 Takifugu rubripes calcium voltage-gated channel subunit alpha1 D (cacna1d), mRNA.

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CloneID	OTo63162
Clone ID Related Accession (Same CDS sequence)	XM_029833248.1
Accession Version	XM_029833248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	voltage-dependent L-type calcium channel subunit alpha-1D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042287.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTA TTATCAGGGG AAGACCCCTG AGGGGGGCAC AGTATGCCGG CAGCAGAAGG 
ACCACTGTCT CCACCAACGC CTCAGCACTC TGGGCAGAAA ACCAAACCTC TTTGCACCAG 
CTCCACCCGT CAGAGCACCC ACAGGCTCCA GCTAGCACCG GCAAGCCTGC GGGCCCCTAC 
CCTGGACTTC TCGGGACCAA CAGTGGCATT CCGACCCACG GAGGTGCCGG TTCAGGCGTC 
AACCCTGTCC TCGCTTGGCA TGCCGCCATC AACGCCGCAC GCCAAGCGCA AGGCGACGGT 
GCAAAGCATG ACGTGAGCTC GCCGCCACCT GTCTGCACCA CTGGACCACC TCCCGTGGGC 
TCGCTGGCGC AGAAGAAGAG GCAGCAGTAC GCCAAAAGCA AAAAGCAGGG AGGCTCTACC 
AACAGCAGGC CACCGAGAGC CCTGTTCTGC CTCACCCTCA ACAACCCCAT CCGCAGGGCC 
TGCATCAGCC TGGTGGAGTG GAAACCATTT GATATCTTTA TACTTCTGTC TATTTTTGCC 
AACTGCGTGG CCTTAGCCAT TTACATCCCC TTTCCTGGAG ACGACTCCAA CTCCACCAAC 
CAAGAACTGG AAACAGTGGA ATATGCCTTC CTGATCATTT TCACTATAGA GACGTTTCTG 
AAGATTATTG CCTACGGCCT GGTCATGCAC CAAAACTCTT ACGTCCGAAA CGGCTGGAAC 
TTGCTGGATT TTGTGATCGT CATCGTTGGG TTGTTCAGTG TGGCTCTGGA GATGATCACC 
AAAGACGCCG ACTCCAGCGG ACAGTCTGGA GGCAAACCCG GGGGGTTCGA TGTCAAGGCC 
CTCCGAGCCT TTCGTGTCCT TCGTCCCCTT CGCCTTGTCT CGGGAGTTCC CAGTTTACAG 
GTGGTTTTAA ACTCCATTAT TAAAGCCATG GTTCCCCTGC TTCACATCGC GCTGCTGGTA 
CTCTTCGTCA TCATCATCTA CGCCGTCATC GGCCTGGAGC TTTTCATTGG CAAAATGCAC 
GCCACGTGCT ACGTGATAGG AACAGGTAAC CTTGCAGAAG AGGAGCCCAC ACCGTGTGCT 
GTGTCCGGGC ATGGCCGCCA CTGCCTGCTC AACGGCACGC TTTGCAGGGA GGGCTGGCAA 
GGTCCCAACA ATGGCATCAC CAACTTCGAC AATTTTTTGT TTGCCATGCT GACTGTGTTC 
CAGTGCATCA CTATGGAGGG CTGGACGGAT GTTCTTTACT GGATGAATGA TGCGATGGGC 
TTCGAGTTGC CGTGGGTCTA CTTTGTCAGC CTGGTCATCT TTGGATCCTT CTTTGTTCTC 
AACCTGGTTT TGGGCGTGTT GAGCGGAGAG TTCTCCAAAG AGCGCGAGAA GGCCAAAGCT 
CGGGGAGATT TCCAAAAGCT ACGCGAGAAG CAGCAGTTAG AGGAAGACCT GAAGGGTTAC 
CTGGACTGGA TCACCCAGGC TGAGGACATC GACCCCGAGA ATGAGGAGGA GGGAGAGGAA 
GATGGCAAAC GCAATCGGGT CACTCTGGCT GACCTCACTG AGAAGAAGAA AGGAAAGTTT 
GGCTGGTTCA CCCAGTCCAC AGAGACACAG GCGAGCATGC CGAGCGAGAA CGAGTCGGCG 
AACACGGACA ACCACGCTGG CGCGGATGCC AAATCGCCCT GCTGTGGCCC GCTGTGTCAA 
AAAATAACTA AGTCAAAATT CAGCCGCCAG TGGAGGCGCT GGAACCGCTT CTGCCGCAGG 
AAGTGCCGCG CCGCTGTGAA ATCCGTCACA TTTTACTGGC TGGTCATCAT CCTGGTGTTC 
CTCAACACTC TCACCATTGC CTCTGAGCAC TACAACCAGC CAGACTGGCT GACCGAAGTC 
CAGGACGTTG CCAACAAAGT CCTCCTGGCC ATGTTCACCC TGGAGATGCT CGTGAAGTTG 
TACAGCCTGG GGCTGCAGGC CTACTTTGTG TCGCTGTTTA ATCGCTTCGA CTGTTTCGTG 
GTGTGCGGCG GCATCGTGGA GACCATCCTG GTGGAGCTCG CTATCATGTC GCCGCTCGGT 
ATCTCCGTCT TCCGCTGCGT ACGACTCCTG CGGATCTTCA AGGTCACGCG ACATTGGGCG 
TCTCTCAGCA ACCTGGTGGC CTCCCTGCTG AACTCCATGA AGTCCATCGC CTCTCTGTTG 
CTGCTGCTCT TCCTCTTCAT CATCATCTTT TCCCTGCTGG GCATGCAGCT TTTCGGGGGG 
AAGTTTAATT TTGACGAGAC TGTGACCAAA AGAAGCACTT TTGACAACTT CCCTCAGGCT 
CTGCTCACTG TGTTCCAGAT CCTGACAGGA GAAGACTGGA ATACTGTGAT GTACGATGGC 
ATCATGGCGT ACGGCGGGCC GGCCTCCTCT GGAATGTTGG TCTCCATTTA CTTCATCATC 
CTCTTCATCT GTGGAAATTA CATCCTGCTA AATGTCTTCT TGGCTATCGC TGTTGACAAC 
CTGGCAGATG CAGAAAGCCT CAACACAGCA CAGAAGGAAG AAGAAGAAGC AAAGAAGAGG 
AGGAACAGTG CCAAGGATAA TAGAGTGGAT CTAATAGAAG GCAAAGAGAC CAAGGCTCTT 
TCACAGGTTC TGTTGATGGA AGACAAGGAG TCTAATCCTG AAGTCAACTC ACCAGTTTCT 
GATGATGATG AGGAGGAGGA GAATTATGAG GACCTCCCAG AAGTCCCACC GGGGCCTCGC 
CCTCAGAGGT TGTCTGAACT CACCATCAAG GAGAAGATTC CACCCATCCC AGCAGGAAGC 
GCCTTCTTCG TCTTCAGTAG CACCAACCCG TTCCGTGTGT TCTGCCACAA ACTGATCAAC 
CACCAGATCT TCACCAACCT CATCTTGGTC TTCATCATGC TCAGCTCCGT GTCCCTGGCT 
GCCGAGGACC CCATACGCAA CTTCTCGGCC CGCAACATCA TACTTGGTTA TTTTGACTAT 
GCTTTCACCG CAATCTTTAC TGTTGAGATC CTGTTGAAGA TCCTAGGCTA TGCAGACTAT 
GTCTTCACTA GTATGTTTAC ATTTGAGATC CTTATTAAGA TGACAGCGTT CGGAGCGTTT 
CTCCATCAAG GAGCATTTTG TCGGAACTAC TTCAACCTTT TGGATCTGCT GGTGGTCGGA 
GTGTCTCTGG TCTCCTTCGG CATTCAGTCC TCTGCTATCT CAGTCGTGAA GATCCTTCGT 
GTTTTAAGAG TCCTTCGTCC ACTCAGGGCC ATCAATAGAG CCAAAGGCCT CAAGCACGTG 
GTCCAGTGTG TCTTCGTGGC CATCAGGACC ATCGGTAACA TCATGATTGT CACCACGCTG 
CTGCAGTTCA TGTTCGCCTG TATCGGAGTG CAGCTCTTTA AAGGGAAATT TTATCGCTGC 
ACCGATGATG CAAAGTCCAG TCCAGAGGAG TGCAAAGGCA CCTACATTCT GTACAACAAC 
GGGGATGCAG CGCTGCCTAT GGCGAAGGAG AGGATCTGGT ACAACAGCGA CTTCAACTTT 
GACAATGTCC TCATGGCCAT GATGGCCCTC TTTACTGTCT CCACCTTTGA AGGATGGCCC 
ACGCTTCTGT ACAAGGCTAT TGACTCCAAC AAAGAGAACA TGGGTCCCAT CTACAACTAC 
CGCATCGAGA TCTCCATCTT CTTCATCATC TACATCATCA TCATCGCTTT CTTCATGATG 
AACATCTTTG TCGGTTTTGT TATTGTCACC TTTCAGGAGC AGGGGGAGAA AGAATACAAA 
AACTGCGAAC TGGACAAGAA CCAGCGTCAG TGTGTGGAAT ACGCTCTTAA AGCTCGTCCA 
CTGCGGAGAT ATATCCCCAA AAACCCGTAC CAGTACAAGT TCTGGTACGT GGTGAACTCT 
ACTGGCTTCG AGTATGTCAT GTTCATACTT ATCATCCTCA ACACCTTGTG TCTGGCCATT 
CAGCACCATG GTCAGTCTCA TTTGTTCAAC TATGCCATGG ACATCCTCAA CATGGTTTTC 
ACTGGGGTTT TCACTGTGGA GATGATCCTC AAGCTCATTG CCTTCAAACC CAGAGGCTAC 
GTCAGGGACC CCTGGAACGT CTTCGACGCT CTAGTGGTGA TCGGCAGTGT AGTCGACATC 
ATACTCAGCC AGAACTATTT TGCTGATGCA TGGAACACAT TTGATGCCTT AATTGTTGTG 
GGTAGCGTGG TTGATATTGC CATCACTGAG ATCAATTCGG CAGCCATTCC TGTCGTCAAG 
GTGATAGTGG ATCCAGGGAA CACGGAGGAC AGCGCTCGCA TCTCAATCAC CTTCTTCCGC 
TTATTTAGAG TGATGAGGCT GGTCAAGCTC CTGAGCAGAG GGGAGGGGAT CAGGACTCTT 
CTGTGGACCT TTATTAAATC CTTCCAGGCC CTTCCCTACG TCGCGCTCCT GATAGCCATG 
CTCTTCTTCA TCTATGCTGT AATCGGAATG CAGGTTTTTG GGAAAATAGC CATGGTCGAC 
GGAACGCAAA TCAATCGCAA CAACAACTTT CAAACCTTCC CTCAAGCTGT CCTGATGCTC 
TTCAGGTGCG CCACAGGTGA GGCCTGGCAG GAGATCATGC TGGCCTGCCT GCCAGGGAAG 
CTTTGCGACT CAGAGTCAGA CTACAACCCC GGCGAGGAGC GAACCTGCGG CAGCGGCTTC 
GCCATCTTCT ACTTCATCAG CTTTTACATG CTCTGTGCTT TTCTGATCAT TAATCTATTC 
GTAGCCGTCA TCATGGATAA CTTTGACTAC CTGACGCGTG ATTGGTCAAT CCTTGGACCA 
CATCATCTCG ATGAGTTTAA AAGGATTTGG TCTGAATACG ACCCTGAAGC CAAGGGTCGG 
ATAAAGCACC TTGATGTGGT GACCCTGTTG AGGAGGATCC AGCCCCCGCT GGGCTTTGGC 
AAGCTTTGTC CTCACAGAGT GGCCTGTAAG CGCCTGGTGG CGATGAACAT GCCCCTCAAC 
AGTGACGGGA CAGTCATGTT TAATGCCACC TTGTTCGCTC TGGTCCGCAC CGCTCTCAAG 
ATTAAGACGG AGGGAAACTT GGATCAGGCC AATGAGGAGC TCAGAGCTGT GATCAAGAAG 
ATCTGGAAGA GGACCAGCAT GAAGCTGCTG GACCAAGTTG TGCCCCCTGC CGGCGATGAC 
GAGGTCACTG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT CAGGAAATTC 
AAGAGGCGCA AAGAGCAAGG TCTTGTGGGT AAGCGCTCGA GGGAGAGGCT CAGCGCCACC 
ATGGCTTTAC AGGCCGGCCT CCGCACCCTG CACGACATTG GACCAGAGAT TCGCAGGGCC 
ATATCATGTG ACCTGCAGCA GGAGGGGCTG GTAGATGGTA ATGAAGAAGA GGAAGAAGAG 
CAAATCTACA GGCGCAACGG AGGCCTTTTT GGGAACTACA TCAACCAACT CAATGCAGAC 
AAACAAGGTT TCTCCAATCT GACCACCGCC ACCCAGCGGC CCTTGCAGAT CCTACCCCTC 
TCCCACACCG CGGTCGAGCC CATGACCACA GTCAGGAGAG CCAGCGGAGG GGAGAGGCAG 
ATCAACGCCT CCCCCATCCA CAATCATTGT CACCACCCTC ACCCCTACAA CCACCCCGCC 
TGCCAGCAGT CACCCATCCC CTCCAATGCC AACCTCAACA ACGCCAACGT GCCCTCACTT 
CTGGCCATGA ACACTGGGAG GCAGCAGAGG TGTTTGTTAA GGCGCGACCG CCCCTCCTCC 
ACTAAGAACG GCTCGTCAGC GTGTACTTAC AGTAAAGGTG TCCATGAGAA ACTGTGGAAG 
TCACACTCAA GCAGTCCACG GAGCAGGACC CTCTGCTTCG AGTCCCACAT TAGGTCAGAA 
AGCAGCCTCA CCGTCTCAAG GGAGGATGTC AGAGACGAGG ACAGCGAGGA GGAGAGAGGC 
TCGGGGGAGT ACTACAGCGG GGAGGAGTTT AATGAAGATG ACATCATGCT TGCGAGGGAC 
AGGTTAGTGA AGCCTGACAA ACTTGACGCC GAGGACGACG CTGCCCTGCA GGTTTCCGCC 
GACGACCACC TCGATGTTTT CTACGATGAC GACCAGCAGC CGATATACCA AACAGACAGG 
CGTTCGCCCA GGAGATGGTT TCTCCCCTCA CCTCAAACCT CAAAAAGGCC GCCATTCAAT 
TTTGAGTGTC TTCGCAAAAA GAGCAGTGAA GAGGAGACCC CCCCGCCACC TTCCCCATCC 
TGCACAGCTC TGCCACTGCA TCTGGTGCAG CAGCAGGTGA TGGCGGTGGC CGGCCTCGAT 
GCCAGCAGAC TGCGCCACCT GTCGCCAGCA CGGTCGCTTT GCTCCTGGGC CACACCCCCC 
GCCTCACCCA TCCACCTTGA CGGCTCCCCT AACTACACGC CCCTGATACA GGTGGACTGG 
CACCGATCCG ATAGCGTGGG CAGCATCCCC ACGGCTGTGA AGAGGAGTTT GTGGTACACG 
CACGGCCAGG AGCTCCCTCC CAGCCGCAGC CATTCTCCGT CACACCTGCA GCTGCCAGCT 
GACGGCCTCT CGCGCTTCCT GCAGAAGAGG GGCAGCGCCA ACAGCTTGGT GGAGGCGGTG 
CTGATCTCCG AGGGTTTGGC AAAATACGCC AAGGACCCCA AATTCGTGTC CGCGACCAAG 
CACGAGATCG CGGACGCCTG CGAGATGACC ATCGACGAGA TGGACAGCGC CGCCAGCAAC 
CTCCTGAACG GCGGGATGGA CAACGGAAGC AGCGGGACAA CCAGCAGCGG TGACTCAGAC 
CCGGACGCGG GTCACCTCAG CGACCGCAGC GTCCGCGACT ACAGCGACGA GGAGCCGAGC 
GCGACCGTCA AGCGTGACGA GGACCTGACC GACGAGATGA TCTGCATCAC CTCGCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029689108.1
CDS	122..6961
Translation	MPFIIRGRPLRGAQYAGSRRTTVSTNASALWAENQTSLHQLHPSEHPQAPASTGKPAGPYPGLLGTNSGIPTHGGAGSGVNPVLAWHAAINAARQAQGDGAKHDVSSPPPVCTTGPPPVGSLAQKKRQQYAKSKKQGGSTNSRPPRALFCLTLNNPIRRACISLVEWKPFDIFILLSIFANCVALAIYIPFPGDDSNSTNQELETVEYAFLIIFTIETFLKIIAYGLVMHQNSYVRNGWNLLDFVIVIVGLFSVALEMITKDADSSGQSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAVIGLELFIGKMHATCYVIGTGNLAEEEPTPCAVSGHGRHCLLNGTLCREGWQGPNNGITNFDNFLFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGEEDGKRNRVTLADLTEKKKGKFGWFTQSTETQASMPSENESANTDNHAGADAKSPCCGPLCQKITKSKFSRQWRRWNRFCRRKCRAAVKSVTFYWLVIILVFLNTLTIASEHYNQPDWLTEVQDVANKVLLAMFTLEMLVKLYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELAIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETVTKRSTFDNFPQALLTVFQILTGEDWNTVMYDGIMAYGGPASSGMLVSIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEEEAKKRRNSAKDNRVDLIEGKETKALSQVLLMEDKESNPEVNSPVSDDDEEEENYEDLPEVPPGPRPQRLSELTIKEKIPPIPAGSAFFVFSSTNPFRVFCHKLINHQIFTNLILVFIMLSSVSLAAEDPIRNFSARNIILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEILIKMTAFGAFLHQGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDDAKSSPEECKGTYILYNNGDAALPMAKERIWYNSDFNFDNVLMAMMALFTVSTFEGWPTLLYKAIDSNKENMGPIYNYRIEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYVMFILIILNTLCLAIQHHGQSHLFNYAMDILNMVFTGVFTVEMILKLIAFKPRGYVRDPWNVFDALVVIGSVVDIILSQNYFADAWNTFDALIVVGSVVDIAITEINSAAIPVVKVIVDPGNTEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLMLFRCATGEAWQEIMLACLPGKLCDSESDYNPGEERTCGSGFAIFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLDQANEELRAVIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKRRKEQGLVGKRSRERLSATMALQAGLRTLHDIGPEIRRAISCDLQQEGLVDGNEEEEEEQIYRRNGGLFGNYINQLNADKQGFSNLTTATQRPLQILPLSHTAVEPMTTVRRASGGERQINASPIHNHCHHPHPYNHPACQQSPIPSNANLNNANVPSLLAMNTGRQQRCLLRRDRPSSTKNGSSACTYSKGVHEKLWKSHSSSPRSRTLCFESHIRSESSLTVSREDVRDEDSEEERGSGEYYSGEEFNEDDIMLARDRLVKPDKLDAEDDAALQVSADDHLDVFYDDDQQPIYQTDRRSPRRWFLPSPQTSKRPPFNFECLRKKSSEEETPPPPSPSCTALPLHLVQQQVMAVAGLDASRLRHLSPARSLCSWATPPASPIHLDGSPNYTPLIQVDWHRSDSVGSIPTAVKRSLWYTHGQELPPSRSHSPSHLQLPADGLSRFLQKRGSANSLVEAVLISEGLAKYAKDPKFVSATKHEIADACEMTIDEMDSAASNLLNGGMDNGSSGTTSSGDSDPDAGHLSDRSVRDYSDEEPSATVKRDEDLTDEMICITSL

Target ORF information:

RefSeq Version	XM_029833248.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes calcium voltage-gated channel subunit alpha1 D (cacna1d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029833248.1

ORF Insert Sequence:

ATGCCGTTTA TTATCAGGGG AAGACCCCTG AGGGGGGCAC AGTATGCCGG CAGCAGAAGG 
ACCACTGTCT CCACCAACGC CTCAGCACTC TGGGCAGAAA ACCAAACCTC TTTGCACCAG 
CTCCACCCGT CAGAGCACCC ACAGGCTCCA GCTAGCACCG GCAAGCCTGC GGGCCCCTAC 
CCTGGACTTC TCGGGACCAA CAGTGGCATT CCGACCCACG GAGGTGCCGG TTCAGGCGTC 
AACCCTGTCC TCGCTTGGCA TGCCGCCATC AACGCCGCAC GCCAAGCGCA AGGCGACGGT 
GCAAAGCATG ACGTGAGCTC GCCGCCACCT GTCTGCACCA CTGGACCACC TCCCGTGGGC 
TCGCTGGCGC AGAAGAAGAG GCAGCAGTAC GCCAAAAGCA AAAAGCAGGG AGGCTCTACC 
AACAGCAGGC CACCGAGAGC CCTGTTCTGC CTCACCCTCA ACAACCCCAT CCGCAGGGCC 
TGCATCAGCC TGGTGGAGTG GAAACCATTT GATATCTTTA TACTTCTGTC TATTTTTGCC 
AACTGCGTGG CCTTAGCCAT TTACATCCCC TTTCCTGGAG ACGACTCCAA CTCCACCAAC 
CAAGAACTGG AAACAGTGGA ATATGCCTTC CTGATCATTT TCACTATAGA GACGTTTCTG 
AAGATTATTG CCTACGGCCT GGTCATGCAC CAAAACTCTT ACGTCCGAAA CGGCTGGAAC 
TTGCTGGATT TTGTGATCGT CATCGTTGGG TTGTTCAGTG TGGCTCTGGA GATGATCACC 
AAAGACGCCG ACTCCAGCGG ACAGTCTGGA GGCAAACCCG GGGGGTTCGA TGTCAAGGCC 
CTCCGAGCCT TTCGTGTCCT TCGTCCCCTT CGCCTTGTCT CGGGAGTTCC CAGTTTACAG 
GTGGTTTTAA ACTCCATTAT TAAAGCCATG GTTCCCCTGC TTCACATCGC GCTGCTGGTA 
CTCTTCGTCA TCATCATCTA CGCCGTCATC GGCCTGGAGC TTTTCATTGG CAAAATGCAC 
GCCACGTGCT ACGTGATAGG AACAGGTAAC CTTGCAGAAG AGGAGCCCAC ACCGTGTGCT 
GTGTCCGGGC ATGGCCGCCA CTGCCTGCTC AACGGCACGC TTTGCAGGGA GGGCTGGCAA 
GGTCCCAACA ATGGCATCAC CAACTTCGAC AATTTTTTGT TTGCCATGCT GACTGTGTTC 
CAGTGCATCA CTATGGAGGG CTGGACGGAT GTTCTTTACT GGATGAATGA TGCGATGGGC 
TTCGAGTTGC CGTGGGTCTA CTTTGTCAGC CTGGTCATCT TTGGATCCTT CTTTGTTCTC 
AACCTGGTTT TGGGCGTGTT GAGCGGAGAG TTCTCCAAAG AGCGCGAGAA GGCCAAAGCT 
CGGGGAGATT TCCAAAAGCT ACGCGAGAAG CAGCAGTTAG AGGAAGACCT GAAGGGTTAC 
CTGGACTGGA TCACCCAGGC TGAGGACATC GACCCCGAGA ATGAGGAGGA GGGAGAGGAA 
GATGGCAAAC GCAATCGGGT CACTCTGGCT GACCTCACTG AGAAGAAGAA AGGAAAGTTT 
GGCTGGTTCA CCCAGTCCAC AGAGACACAG GCGAGCATGC CGAGCGAGAA CGAGTCGGCG 
AACACGGACA ACCACGCTGG CGCGGATGCC AAATCGCCCT GCTGTGGCCC GCTGTGTCAA 
AAAATAACTA AGTCAAAATT CAGCCGCCAG TGGAGGCGCT GGAACCGCTT CTGCCGCAGG 
AAGTGCCGCG CCGCTGTGAA ATCCGTCACA TTTTACTGGC TGGTCATCAT CCTGGTGTTC 
CTCAACACTC TCACCATTGC CTCTGAGCAC TACAACCAGC CAGACTGGCT GACCGAAGTC 
CAGGACGTTG CCAACAAAGT CCTCCTGGCC ATGTTCACCC TGGAGATGCT CGTGAAGTTG 
TACAGCCTGG GGCTGCAGGC CTACTTTGTG TCGCTGTTTA ATCGCTTCGA CTGTTTCGTG 
GTGTGCGGCG GCATCGTGGA GACCATCCTG GTGGAGCTCG CTATCATGTC GCCGCTCGGT 
ATCTCCGTCT TCCGCTGCGT ACGACTCCTG CGGATCTTCA AGGTCACGCG ACATTGGGCG 
TCTCTCAGCA ACCTGGTGGC CTCCCTGCTG AACTCCATGA AGTCCATCGC CTCTCTGTTG 
CTGCTGCTCT TCCTCTTCAT CATCATCTTT TCCCTGCTGG GCATGCAGCT TTTCGGGGGG 
AAGTTTAATT TTGACGAGAC TGTGACCAAA AGAAGCACTT TTGACAACTT CCCTCAGGCT 
CTGCTCACTG TGTTCCAGAT CCTGACAGGA GAAGACTGGA ATACTGTGAT GTACGATGGC 
ATCATGGCGT ACGGCGGGCC GGCCTCCTCT GGAATGTTGG TCTCCATTTA CTTCATCATC 
CTCTTCATCT GTGGAAATTA CATCCTGCTA AATGTCTTCT TGGCTATCGC TGTTGACAAC 
CTGGCAGATG CAGAAAGCCT CAACACAGCA CAGAAGGAAG AAGAAGAAGC AAAGAAGAGG 
AGGAACAGTG CCAAGGATAA TAGAGTGGAT CTAATAGAAG GCAAAGAGAC CAAGGCTCTT 
TCACAGGTTC TGTTGATGGA AGACAAGGAG TCTAATCCTG AAGTCAACTC ACCAGTTTCT 
GATGATGATG AGGAGGAGGA GAATTATGAG GACCTCCCAG AAGTCCCACC GGGGCCTCGC 
CCTCAGAGGT TGTCTGAACT CACCATCAAG GAGAAGATTC CACCCATCCC AGCAGGAAGC 
GCCTTCTTCG TCTTCAGTAG CACCAACCCG TTCCGTGTGT TCTGCCACAA ACTGATCAAC 
CACCAGATCT TCACCAACCT CATCTTGGTC TTCATCATGC TCAGCTCCGT GTCCCTGGCT 
GCCGAGGACC CCATACGCAA CTTCTCGGCC CGCAACATCA TACTTGGTTA TTTTGACTAT 
GCTTTCACCG CAATCTTTAC TGTTGAGATC CTGTTGAAGA TCCTAGGCTA TGCAGACTAT 
GTCTTCACTA GTATGTTTAC ATTTGAGATC CTTATTAAGA TGACAGCGTT CGGAGCGTTT 
CTCCATCAAG GAGCATTTTG TCGGAACTAC TTCAACCTTT TGGATCTGCT GGTGGTCGGA 
GTGTCTCTGG TCTCCTTCGG CATTCAGTCC TCTGCTATCT CAGTCGTGAA GATCCTTCGT 
GTTTTAAGAG TCCTTCGTCC ACTCAGGGCC ATCAATAGAG CCAAAGGCCT CAAGCACGTG 
GTCCAGTGTG TCTTCGTGGC CATCAGGACC ATCGGTAACA TCATGATTGT CACCACGCTG 
CTGCAGTTCA TGTTCGCCTG TATCGGAGTG CAGCTCTTTA AAGGGAAATT TTATCGCTGC 
ACCGATGATG CAAAGTCCAG TCCAGAGGAG TGCAAAGGCA CCTACATTCT GTACAACAAC 
GGGGATGCAG CGCTGCCTAT GGCGAAGGAG AGGATCTGGT ACAACAGCGA CTTCAACTTT 
GACAATGTCC TCATGGCCAT GATGGCCCTC TTTACTGTCT CCACCTTTGA AGGATGGCCC 
ACGCTTCTGT ACAAGGCTAT TGACTCCAAC AAAGAGAACA TGGGTCCCAT CTACAACTAC 
CGCATCGAGA TCTCCATCTT CTTCATCATC TACATCATCA TCATCGCTTT CTTCATGATG 
AACATCTTTG TCGGTTTTGT TATTGTCACC TTTCAGGAGC AGGGGGAGAA AGAATACAAA 
AACTGCGAAC TGGACAAGAA CCAGCGTCAG TGTGTGGAAT ACGCTCTTAA AGCTCGTCCA 
CTGCGGAGAT ATATCCCCAA AAACCCGTAC CAGTACAAGT TCTGGTACGT GGTGAACTCT 
ACTGGCTTCG AGTATGTCAT GTTCATACTT ATCATCCTCA ACACCTTGTG TCTGGCCATT 
CAGCACCATG GTCAGTCTCA TTTGTTCAAC TATGCCATGG ACATCCTCAA CATGGTTTTC 
ACTGGGGTTT TCACTGTGGA GATGATCCTC AAGCTCATTG CCTTCAAACC CAGAGGCTAC 
GTCAGGGACC CCTGGAACGT CTTCGACGCT CTAGTGGTGA TCGGCAGTGT AGTCGACATC 
ATACTCAGCC AGAACTATTT TGCTGATGCA TGGAACACAT TTGATGCCTT AATTGTTGTG 
GGTAGCGTGG TTGATATTGC CATCACTGAG ATCAATTCGG CAGCCATTCC TGTCGTCAAG 
GTGATAGTGG ATCCAGGGAA CACGGAGGAC AGCGCTCGCA TCTCAATCAC CTTCTTCCGC 
TTATTTAGAG TGATGAGGCT GGTCAAGCTC CTGAGCAGAG GGGAGGGGAT CAGGACTCTT 
CTGTGGACCT TTATTAAATC CTTCCAGGCC CTTCCCTACG TCGCGCTCCT GATAGCCATG 
CTCTTCTTCA TCTATGCTGT AATCGGAATG CAGGTTTTTG GGAAAATAGC CATGGTCGAC 
GGAACGCAAA TCAATCGCAA CAACAACTTT CAAACCTTCC CTCAAGCTGT CCTGATGCTC 
TTCAGGTGCG CCACAGGTGA GGCCTGGCAG GAGATCATGC TGGCCTGCCT GCCAGGGAAG 
CTTTGCGACT CAGAGTCAGA CTACAACCCC GGCGAGGAGC GAACCTGCGG CAGCGGCTTC 
GCCATCTTCT ACTTCATCAG CTTTTACATG CTCTGTGCTT TTCTGATCAT TAATCTATTC 
GTAGCCGTCA TCATGGATAA CTTTGACTAC CTGACGCGTG ATTGGTCAAT CCTTGGACCA 
CATCATCTCG ATGAGTTTAA AAGGATTTGG TCTGAATACG ACCCTGAAGC CAAGGGTCGG 
ATAAAGCACC TTGATGTGGT GACCCTGTTG AGGAGGATCC AGCCCCCGCT GGGCTTTGGC 
AAGCTTTGTC CTCACAGAGT GGCCTGTAAG CGCCTGGTGG CGATGAACAT GCCCCTCAAC 
AGTGACGGGA CAGTCATGTT TAATGCCACC TTGTTCGCTC TGGTCCGCAC CGCTCTCAAG 
ATTAAGACGG AGGGAAACTT GGATCAGGCC AATGAGGAGC TCAGAGCTGT GATCAAGAAG 
ATCTGGAAGA GGACCAGCAT GAAGCTGCTG GACCAAGTTG TGCCCCCTGC CGGCGATGAC 
GAGGTCACTG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT CAGGAAATTC 
AAGAGGCGCA AAGAGCAAGG TCTTGTGGGT AAGCGCTCGA GGGAGAGGCT CAGCGCCACC 
ATGGCTTTAC AGGCCGGCCT CCGCACCCTG CACGACATTG GACCAGAGAT TCGCAGGGCC 
ATATCATGTG ACCTGCAGCA GGAGGGGCTG GTAGATGGTA ATGAAGAAGA GGAAGAAGAG 
CAAATCTACA GGCGCAACGG AGGCCTTTTT GGGAACTACA TCAACCAACT CAATGCAGAC 
AAACAAGGTT TCTCCAATCT GACCACCGCC ACCCAGCGGC CCTTGCAGAT CCTACCCCTC 
TCCCACACCG CGGTCGAGCC CATGACCACA GTCAGGAGAG CCAGCGGAGG GGAGAGGCAG 
ATCAACGCCT CCCCCATCCA CAATCATTGT CACCACCCTC ACCCCTACAA CCACCCCGCC 
TGCCAGCAGT CACCCATCCC CTCCAATGCC AACCTCAACA ACGCCAACGT GCCCTCACTT 
CTGGCCATGA ACACTGGGAG GCAGCAGAGG TGTTTGTTAA GGCGCGACCG CCCCTCCTCC 
ACTAAGAACG GCTCGTCAGC GTGTACTTAC AGTAAAGGTG TCCATGAGAA ACTGTGGAAG 
TCACACTCAA GCAGTCCACG GAGCAGGACC CTCTGCTTCG AGTCCCACAT TAGGTCAGAA 
AGCAGCCTCA CCGTCTCAAG GGAGGATGTC AGAGACGAGG ACAGCGAGGA GGAGAGAGGC 
TCGGGGGAGT ACTACAGCGG GGAGGAGTTT AATGAAGATG ACATCATGCT TGCGAGGGAC 
AGGTTAGTGA AGCCTGACAA ACTTGACGCC GAGGACGACG CTGCCCTGCA GGTTTCCGCC 
GACGACCACC TCGATGTTTT CTACGATGAC GACCAGCAGC CGATATACCA AACAGACAGG 
CGTTCGCCCA GGAGATGGTT TCTCCCCTCA CCTCAAACCT CAAAAAGGCC GCCATTCAAT 
TTTGAGTGTC TTCGCAAAAA GAGCAGTGAA GAGGAGACCC CCCCGCCACC TTCCCCATCC 
TGCACAGCTC TGCCACTGCA TCTGGTGCAG CAGCAGGTGA TGGCGGTGGC CGGCCTCGAT 
GCCAGCAGAC TGCGCCACCT GTCGCCAGCA CGGTCGCTTT GCTCCTGGGC CACACCCCCC 
GCCTCACCCA TCCACCTTGA CGGCTCCCCT AACTACACGC CCCTGATACA GGTGGACTGG 
CACCGATCCG ATAGCGTGGG CAGCATCCCC ACGGCTGTGA AGAGGAGTTT GTGGTACACG 
CACGGCCAGG AGCTCCCTCC CAGCCGCAGC CATTCTCCGT CACACCTGCA GCTGCCAGCT 
GACGGCCTCT CGCGCTTCCT GCAGAAGAGG GGCAGCGCCA ACAGCTTGGT GGAGGCGGTG 
CTGATCTCCG AGGGTTTGGC AAAATACGCC AAGGACCCCA AATTCGTGTC CGCGACCAAG 
CACGAGATCG CGGACGCCTG CGAGATGACC ATCGACGAGA TGGACAGCGC CGCCAGCAAC 
CTCCTGAACG GCGGGATGGA CAACGGAAGC AGCGGGACAA CCAGCAGCGG TGACTCAGAC 
CCGGACGCGG GTCACCTCAG CGACCGCAGC GTCCGCGACT ACAGCGACGA GGAGCCGAGC 
GCGACCGTCA AGCGTGACGA GGACCTGACC GACGAGATGA TCTGCATCAC CTCGCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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cacna1d cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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