NOS2 cDNA ORF clone, Taeniopygia guttata(zebra finch)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS2
Entrez Gene ID	100230954
Full Name	nitric oxide synthase 2
Gene Type	protein-coding
Organism	Taeniopygia guttata(zebra finch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012568691.1	XP_012424145.1	nitric oxide synthase, inducible
XM_030287970.1	XP_030143830.1	nitric oxide synthase, inducible isoform X1
XM_030287971.1	XP_030143831.1	nitric oxide synthase, inducible isoform X1
XM_030287974.1	XP_030143834.1	nitric oxide synthase, inducible isoform X2
XM_030287973.1	XP_030143833.1	nitric oxide synthase, inducible isoform X1

Pathways from BioSystems
Gene Ontology

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTd19876	XM_012568691.1 Latest version!	Taeniopygia guttata nitric oxide synthase 2, inducible (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTd31999	XM_030287970.1 Latest version!	Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTd31999	XM_030287971.1 Latest version!	Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTd31999	XM_030287973.1 Latest version!	Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTd32000	XM_030287974.1 Latest version!	Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS2 ( XM_012568691.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_012424145.1 Taeniopygia guttata nitric oxide synthase 2, inducible (NOS2), mRNA.

Price & Availability↑

CloneID	OTd19876
Clone ID Related Accession (Same CDS sequence)	XM_012568691.1
Accession Version	XM_012568691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1432483200000
Organism	Taeniopygia guttata(zebra finch)
Product	nitric oxide synthase, inducible
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002197521.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Taeniopygia guttata Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC TGCCTATCAT AACTTCAGCA AAGCCCAGTG AGGGAGGAGA GAATCTCCCT 
TCAAATGACA TCAAACTTTC AAACCAAGTA TCAGGATGTC CAAGACATGT AAGAGTAAGA 
AACTTGGAAA ATGGATCCAG CTTTCTTGAC ACACTACACC TGACTGCAAA AGAGGTTATC 
AATTGCCAGA CCAAAGCCTG CCAAGGGTCA CTCATGACCC CAAAGGGCCT GGTGAGAGGT 
ACCAGAGATG GGCCGGTTCC ACTACAGGAT CTTTTACCTC AGGCAATAGA CTTCCTCAAG 
CAATACTACA GTTCATTTAA AGAGCCAAAA ATCAAAGAAG AGCTGGGCCG GCTGGAAGCA 
GTGACCAAGG AGATAGAAAC AACAGGAACT TACCACCTGA CAAAGGATGA GTTGGCCTTT 
GCTGCCAAGC AGGCCTGGAG GAATGCCCCC AGGTGCATTG GGAGGATCCA GTGGTCCAAC 
CTGCAGGTGT TTGATGCACG AGACTGTAAA ACAGCCAAGG AAATGTTTGA GCATATCTGT 
CATCATATTC AGTATGCCAC AAACAATGGT AATATAAGGT CAGCCATCAC GGTGTTCCCG 
CACAGGACGG ACGGGCAGCA CGATTTCCGT GTGTGGAACA GCCAGCTGAT CCGCTACGCT 
GGCTACCAAA TGTCAGATGG CTCTGTCCTT GGAGACCCTG CTGCTGTGGA GTTCACTCAG 
TTGTGCATTG AACTTGGGTG GAAGCCGAAA TATGGCCGCT TTGATGTACT TCCACTTGTT 
CTCCAAGCAA ATGGCCAAGA CCCAGAAATA TTTGAATTAC CTCCAGAAAT TGTCCTCCAA 
GTGCCAATGG AGCATCCAAA GTACAAGTGG TTTAAGGAGC TGGAGCTGAA GTGGTACGCG 
CTGCCTGCTG TGGCCAACAT GCTTCTGGAG GCAGGAGGGC TGGAGTTCCC CGCATGCCCT 
TTCAATGGCT GGTACATGGG GACAGAGATC GGCGTGCGGG ACTTCTGCGA CGCGCAGCGC 
TACAACATCC TGAAGGAGGT AGGGAGGAGA ATGGGACTGG AAACAAATAA ACTGTCATCA 
TTATGGAAGG ACCGAGCTGT TGTAGAGATC AATGTGGCTG TGCTTCACAG CTTCCAGAGA 
CAGAATGTAA CCATCATGGA TCACCACTCA GCCTCCGAGT CCTTCCTGAA GTACATGCAG 
AGCGAGTACC GCGCGCGGGG CGGCTGCCCG GCAGATTGGG TGTGGATCGT GCCTCCCATG 
TCAGGCAGCA TAACCCCCGT GTTCCACCAA GAGATGTTGA ACTATGTCCT CACTCCCTTC 
TATTACTACC AGGTAGATGC ATGGAGAACA CATGTATGGC ATGATGAGAC CCGAAGGCGA 
AAGAAAAAAG AAATAAAGTT TAGCATCTTG GCAAAGGCTG TGCTCTTTGC ATCTTCACTG 
ATGCGAGGGG CAGTGGCCAC CAGGGCCAAG GTCACCGTGA TCTACGCGAC AGAAACGGGC 
AAGTCGGAGA CGCTCGCCAA CAGCCTGTGC AGCCTGTTCA GCTGTGCCTT CAGCACCAAG 
ATCCTCTGCA TGGATGAGTA CAATATTTGT GACCTGGAAA AAGAAACACT TCTTTTAGTG 
GTTACTAGCA CTTTTGGAAA TGGAGATTCT CCAGGTAACG GAAAGACCTT GAAGGACTCT 
TTGCTCAGAC TAAAACTGCT GAGAAATAAA ATTAGGTATG CTGTGTTTGG TCTGGGGTCC 
AGCATGTACC CAGAGTTCTG TGCCTTTGCT CATGCCATTG ACCAAAAACT GGCCCAACTC 
GGGGCTTCCC AGCTCACTCC AGTGGGTGAA GGGGATGAAC TCAATGGGCA AGAAGAAGCT 
TTCCGCAGCT GGGCAGTCAG TGCTTTCAAG GCTGCCTGTG ACATCTTTAA CGTCCGTGGG 
AGACACAGTA TTCAGTTGCC TAAGATCTAC ACCTCTGAGG AAAGGTGGGA CCCTACCAAT 
TACAGGCTAG TGCATGACTC CCAGCCCATG GACCTGGCTA AAGCTCTTGC AAACATTCAT 
GCCAAGGATG TAATTCCCAT GAAGCTGAAA TTTAGGCAGA ATCTTCAAAG TTTGAAGTCC 
AGTCGTGTCA CCATTCTGGT TAAACTTCAC TGTGAGACAG ATCAGGAAGT GCACTACCTT 
CCCGGGGAGC ACATCGGGAT TTTCCCAGGC AACCAGGCAG AATTAGTCCA TGGCATTATT 
GCACATGTCA AGGATGCCCC TCCAGCTGAT CAGACTATCA GGCTTGAAAC CTGCATTGAT 
GGTGGGTACT GGGCAAGTCA CAAAAAGATT CCAGCCTGCA CACTACCTGA GGCTTTGACA 
TACTTGCTTG ATATCACTAC TCCACCCTCC CAACAACTGC TAAAAAAACT CTCCCAGCTG 
GTGACAGCAG AAGGAGATAA GCAAAGGCTG GAGGTGCTGT GTCAGGTGAA AGAATACAAT 
AAATGGAAGT TTTACAACAG CCCAAACATC CTGGAGGTGC TGGAGGAGTT CCCCTCTGCT 
GAGGTCTCTG CAGCTTTCCT GCTGACTCAG CTGCCACTTC TGAAGCCCAG GTACTATTCT 
GTCAGCTCCT CCTGTGACCT GACAGCCAGA GAGATTCATC TGACAGTTGC AGTTGTAAAC 
TACAGGACAA GAGATGGTGA AGGGCCTTTG CACCATGGAG TCTGCAGCAC CTGGCTGAAC 
ACAATAGAGC TCAACGAAAC CGTTCCCTGC TTCATCCGCA GTGCTAATGA GTTCCACCTC 
CCGGAGGAGC CATCCAAGCC CTGCATTCTG ATCGGCTCGG GCACGGGGAT TGCTCCCTTC 
AGGAGCTTCT GGCTGCAGCG CCTCTATGAC CTGGAGCACA GAGGGCTCAG AGGAGGGGAC 
ATGACCCTGC TGTTCGGCTG CCGGCATCCA GACCTGGATC ACATCTACAG GGAAGAGACT 
GAGGAGATGA AGAGGAAGGG AGTCCTGAGT GATGTCTACA CAGCTTACTC CAGACTGCCT 
GGCCAAGAGA AAGTGTACGT CCAGGACGTG CTGCAGGGGC AGCTGGCGGC CCGCGTGTGC 
GAGGTGCTGC AGGGGCAGCA GGGCCATGTG TACGTGTGCG GGGACGTGCG CATGGCGCGG 
GACGTGGCCG GGGCGCTGCG GGCGCTGCTC GCCCGCGCCC TGCGCCTCAG CCAGCAGCAG 
GCCAGCGAGT TCCTGCAGCA GCTCAAGAGC CAAAAGCGAT ACCATGAAGA TATATTTGGG 
GCTGTGTTCG CACATGAAGT CAAAAGAGCT TTGCAACCCA CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012424145.1
CDS	106..3393
Translation	MERLPIITSAKPSEGGENLPSNDIKLSNQVSGCPRHVRVRNLENGSSFLDTLHLTAKEVINCQTKACQGSLMTPKGLVRGTRDGPVPLQDLLPQAIDFLKQYYSSFKEPKIKEELGRLEAVTKEIETTGTYHLTKDELAFAAKQAWRNAPRCIGRIQWSNLQVFDARDCKTAKEMFEHICHHIQYATNNGNIRSAITVFPHRTDGQHDFRVWNSQLIRYAGYQMSDGSVLGDPAAVEFTQLCIELGWKPKYGRFDVLPLVLQANGQDPEIFELPPEIVLQVPMEHPKYKWFKELELKWYALPAVANMLLEAGGLEFPACPFNGWYMGTEIGVRDFCDAQRYNILKEVGRRMGLETNKLSSLWKDRAVVEINVAVLHSFQRQNVTIMDHHSASESFLKYMQSEYRARGGCPADWVWIVPPMSGSITPVFHQEMLNYVLTPFYYYQVDAWRTHVWHDETRRRKKKEIKFSILAKAVLFASSLMRGAVATRAKVTVIYATETGKSETLANSLCSLFSCAFSTKILCMDEYNICDLEKETLLLVVTSTFGNGDSPGNGKTLKDSLLRLKLLRNKIRYAVFGLGSSMYPEFCAFAHAIDQKLAQLGASQLTPVGEGDELNGQEEAFRSWAVSAFKAACDIFNVRGRHSIQLPKIYTSEERWDPTNYRLVHDSQPMDLAKALANIHAKDVIPMKLKFRQNLQSLKSSRVTILVKLHCETDQEVHYLPGEHIGIFPGNQAELVHGIIAHVKDAPPADQTIRLETCIDGGYWASHKKIPACTLPEALTYLLDITTPPSQQLLKKLSQLVTAEGDKQRLEVLCQVKEYNKWKFYNSPNILEVLEEFPSAEVSAAFLLTQLPLLKPRYYSVSSSCDLTAREIHLTVAVVNYRTRDGEGPLHHGVCSTWLNTIELNETVPCFIRSANEFHLPEEPSKPCILIGSGTGIAPFRSFWLQRLYDLEHRGLRGGDMTLLFGCRHPDLDHIYREETEEMKRKGVLSDVYTAYSRLPGQEKVYVQDVLQGQLAARVCEVLQGQQGHVYVCGDVRMARDVAGALRALLARALRLSQQQASEFLQQLKSQKRYHEDIFGAVFAHEVKRALQPTS

Target ORF information:

RefSeq Version	XM_012568691.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata nitric oxide synthase 2, inducible (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012568691.1

ORF Insert Sequence:

ATGGAGAGGC TGCCTATCAT AACTTCAGCA AAGCCCAGTG AGGGAGGAGA GAATCTCCCT 
TCAAATGACA TCAAACTTTC AAACCAAGTA TCAGGATGTC CAAGACATGT AAGAGTAAGA 
AACTTGGAAA ATGGATCCAG CTTTCTTGAC ACACTACACC TGACTGCAAA AGAGGTTATC 
AATTGCCAGA CCAAAGCCTG CCAAGGGTCA CTCATGACCC CAAAGGGCCT GGTGAGAGGT 
ACCAGAGATG GGCCGGTTCC ACTACAGGAT CTTTTACCTC AGGCAATAGA CTTCCTCAAG 
CAATACTACA GTTCATTTAA AGAGCCAAAA ATCAAAGAAG AGCTGGGCCG GCTGGAAGCA 
GTGACCAAGG AGATAGAAAC AACAGGAACT TACCACCTGA CAAAGGATGA GTTGGCCTTT 
GCTGCCAAGC AGGCCTGGAG GAATGCCCCC AGGTGCATTG GGAGGATCCA GTGGTCCAAC 
CTGCAGGTGT TTGATGCACG AGACTGTAAA ACAGCCAAGG AAATGTTTGA GCATATCTGT 
CATCATATTC AGTATGCCAC AAACAATGGT AATATAAGGT CAGCCATCAC GGTGTTCCCG 
CACAGGACGG ACGGGCAGCA CGATTTCCGT GTGTGGAACA GCCAGCTGAT CCGCTACGCT 
GGCTACCAAA TGTCAGATGG CTCTGTCCTT GGAGACCCTG CTGCTGTGGA GTTCACTCAG 
TTGTGCATTG AACTTGGGTG GAAGCCGAAA TATGGCCGCT TTGATGTACT TCCACTTGTT 
CTCCAAGCAA ATGGCCAAGA CCCAGAAATA TTTGAATTAC CTCCAGAAAT TGTCCTCCAA 
GTGCCAATGG AGCATCCAAA GTACAAGTGG TTTAAGGAGC TGGAGCTGAA GTGGTACGCG 
CTGCCTGCTG TGGCCAACAT GCTTCTGGAG GCAGGAGGGC TGGAGTTCCC CGCATGCCCT 
TTCAATGGCT GGTACATGGG GACAGAGATC GGCGTGCGGG ACTTCTGCGA CGCGCAGCGC 
TACAACATCC TGAAGGAGGT AGGGAGGAGA ATGGGACTGG AAACAAATAA ACTGTCATCA 
TTATGGAAGG ACCGAGCTGT TGTAGAGATC AATGTGGCTG TGCTTCACAG CTTCCAGAGA 
CAGAATGTAA CCATCATGGA TCACCACTCA GCCTCCGAGT CCTTCCTGAA GTACATGCAG 
AGCGAGTACC GCGCGCGGGG CGGCTGCCCG GCAGATTGGG TGTGGATCGT GCCTCCCATG 
TCAGGCAGCA TAACCCCCGT GTTCCACCAA GAGATGTTGA ACTATGTCCT CACTCCCTTC 
TATTACTACC AGGTAGATGC ATGGAGAACA CATGTATGGC ATGATGAGAC CCGAAGGCGA 
AAGAAAAAAG AAATAAAGTT TAGCATCTTG GCAAAGGCTG TGCTCTTTGC ATCTTCACTG 
ATGCGAGGGG CAGTGGCCAC CAGGGCCAAG GTCACCGTGA TCTACGCGAC AGAAACGGGC 
AAGTCGGAGA CGCTCGCCAA CAGCCTGTGC AGCCTGTTCA GCTGTGCCTT CAGCACCAAG 
ATCCTCTGCA TGGATGAGTA CAATATTTGT GACCTGGAAA AAGAAACACT TCTTTTAGTG 
GTTACTAGCA CTTTTGGAAA TGGAGATTCT CCAGGTAACG GAAAGACCTT GAAGGACTCT 
TTGCTCAGAC TAAAACTGCT GAGAAATAAA ATTAGGTATG CTGTGTTTGG TCTGGGGTCC 
AGCATGTACC CAGAGTTCTG TGCCTTTGCT CATGCCATTG ACCAAAAACT GGCCCAACTC 
GGGGCTTCCC AGCTCACTCC AGTGGGTGAA GGGGATGAAC TCAATGGGCA AGAAGAAGCT 
TTCCGCAGCT GGGCAGTCAG TGCTTTCAAG GCTGCCTGTG ACATCTTTAA CGTCCGTGGG 
AGACACAGTA TTCAGTTGCC TAAGATCTAC ACCTCTGAGG AAAGGTGGGA CCCTACCAAT 
TACAGGCTAG TGCATGACTC CCAGCCCATG GACCTGGCTA AAGCTCTTGC AAACATTCAT 
GCCAAGGATG TAATTCCCAT GAAGCTGAAA TTTAGGCAGA ATCTTCAAAG TTTGAAGTCC 
AGTCGTGTCA CCATTCTGGT TAAACTTCAC TGTGAGACAG ATCAGGAAGT GCACTACCTT 
CCCGGGGAGC ACATCGGGAT TTTCCCAGGC AACCAGGCAG AATTAGTCCA TGGCATTATT 
GCACATGTCA AGGATGCCCC TCCAGCTGAT CAGACTATCA GGCTTGAAAC CTGCATTGAT 
GGTGGGTACT GGGCAAGTCA CAAAAAGATT CCAGCCTGCA CACTACCTGA GGCTTTGACA 
TACTTGCTTG ATATCACTAC TCCACCCTCC CAACAACTGC TAAAAAAACT CTCCCAGCTG 
GTGACAGCAG AAGGAGATAA GCAAAGGCTG GAGGTGCTGT GTCAGGTGAA AGAATACAAT 
AAATGGAAGT TTTACAACAG CCCAAACATC CTGGAGGTGC TGGAGGAGTT CCCCTCTGCT 
GAGGTCTCTG CAGCTTTCCT GCTGACTCAG CTGCCACTTC TGAAGCCCAG GTACTATTCT 
GTCAGCTCCT CCTGTGACCT GACAGCCAGA GAGATTCATC TGACAGTTGC AGTTGTAAAC 
TACAGGACAA GAGATGGTGA AGGGCCTTTG CACCATGGAG TCTGCAGCAC CTGGCTGAAC 
ACAATAGAGC TCAACGAAAC CGTTCCCTGC TTCATCCGCA GTGCTAATGA GTTCCACCTC 
CCGGAGGAGC CATCCAAGCC CTGCATTCTG ATCGGCTCGG GCACGGGGAT TGCTCCCTTC 
AGGAGCTTCT GGCTGCAGCG CCTCTATGAC CTGGAGCACA GAGGGCTCAG AGGAGGGGAC 
ATGACCCTGC TGTTCGGCTG CCGGCATCCA GACCTGGATC ACATCTACAG GGAAGAGACT 
GAGGAGATGA AGAGGAAGGG AGTCCTGAGT GATGTCTACA CAGCTTACTC CAGACTGCCT 
GGCCAAGAGA AAGTGTACGT CCAGGACGTG CTGCAGGGGC AGCTGGCGGC CCGCGTGTGC 
GAGGTGCTGC AGGGGCAGCA GGGCCATGTG TACGTGTGCG GGGACGTGCG CATGGCGCGG 
GACGTGGCCG GGGCGCTGCG GGCGCTGCTC GCCCGCGCCC TGCGCCTCAG CCAGCAGCAG 
GCCAGCGAGT TCCTGCAGCA GCTCAAGAGC CAAAAGCGAT ACCATGAAGA TATATTTGGG 
GCTGTGTTCG CACATGAAGT CAAAAGAGCT TTGCAACCCA CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_030287970.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030143830.1 Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTd31999
Clone ID Related Accession (Same CDS sequence)	XM_030287970.1 , XM_030287971.1 , XM_030287973.1
Accession Version	XM_030287970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	nitric oxide synthase, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCC CATGGCAGTT TGTGTTCAAA TCTCGTGCTG CCAAGACCCA GTACCCCCAG 
GAGAAGGATA TCAACAACAA CGTGGAGAAA AATAGGAAGA TCCATGGCTC AGAAAAGGAT 
GATGCCAAGT TGCACAATCC GAGCAAGAAG CAGATGGAGA GGCTGCCTAT CATAACTTCA 
GCAAAGCCCA GTGAGGGAGG AGAGGTCTGT TCCCAGCATC ATGGTTTGAT GAATCTCCCT 
TCAAATGACA TCAAACTTTC AAACCAAGTA TCAGGATGTC CAAGACATGT AAGAGTAAGA 
AACTTGGAAA ATGGATCCAG CTTTCTTGAC ACACTACACC TGACTGCAAA AGAGGTTATC 
AATTGCCAGA CCAAAGCCTG CCAAGGGTCA CTCATGACCC CAAAGGGCCT GGTGAGAGGT 
ACCAGAGATG GGCCGGTTCC ACTACAGGAT CTTTTACCTC AGGCAATAGA CTTCCTCAAG 
CAATACTACA GTTCATTTAA AGAGCCAAAA ATCAAAGAAG AGCTGGGCCG GCTGGAAGCA 
GTGACCAAGG AGATAGAAAC AACAGGAACT TACCACCTGA CAAAGGATGA GTTGGCCTTT 
GCTGCCAAGC AGGCCTGGAG GAATGCCCCC AGGTGCATTG GGAGGATCCA GTGGTCCAAC 
CTGCAGGTGT TTGATGCACG AGACTGTAAA ACAGCCAAGG AAATGTTTGA GCATATCTGT 
CATCATATTC AGTATGCCAC AAACAATGGT AATATAAGGT CAGCCATCAC GGTGTTCCCG 
CACAGGACGG ACGGGCAGCA CGATTTCCGT GTGTGGAACA GCCAGCTGAT CCGCTACGCT 
GGCTACCAAA TGTCAGATGG CTCTGTCCTT GGAGACCCTG CTGCTGTGGA GTTCACTCAG 
TTGTGCATTG AACTTGGGTG GAAGCCGAAA TATGGCCGCT TTGATGTACT TCCACTTGTT 
CTCCAAGCAA ATGGCCAAGA CCCAGAAATA TTTGAATTAC CTCCAGAAAT TGTCCTCCAA 
GTGCCAATGG AGCATCCAAA GTACAAGTGG TTTAAGGAGC TGGAGCTGAA GTGGTACGCG 
CTGCCTGCTG TGGCCAACAT GCTTCTGGAG GCAGGAGGGC TGGAGTTCCC CGCATGCCCT 
TTCAATGGCT GGTACATGGG GACAGAGATC GGCGTGCGGG ACTTCTGCGA CGCGCAGCGC 
TACAACATCC TGAAGGAGGT AGGGAGGAGA ATGGGACTGG AAACAAATAA ACTGTCATCA 
TTATGGAAGG ACCGAGCTGT TGTAGAGATC AATGTGGCTG TGCTTCACAG CTTCCAGAGA 
CAGAATGTAA CCATCATGGA TCACCACTCA GCCTCCGAGT CCTTCCTGAA GTACATGCAG 
AGCGAGTACC GCGCGCGGGG CGGCTGCCCG GCAGATTGGG TGTGGATCGT GCCTCCCATG 
TCAGGCAGCA TAACCCCCGT GTTCCACCAA GAGATGTTGA ACTATGTCCT CACTCCCTTC 
TATTACTACC AGGTAGATGC ATGGAAAACA CATGTATGGC ATGATGAGAC CCGAAGGCGA 
AAGAAAAAAG AAATAAAGTT TAGCATCTTG GCAAAGGCTG TGCTCTTTGC ATCCTCACTG 
ATGCGAGGGG CAGTGGCCAC CAGGGCCAAG GTCACCGTGA TCTACGCGAC AGAAACGGGC 
AAGTCGGAGA CGCTCGCCAA CAGCCTGTGC AGCCTGTTCA GCTGTGCCTT CAGCACCAAG 
ATCCTCTGCA TGGATGAGTA CAATATTTGT GACCTGGAAA AAGAAACACT TCTTTTAGTG 
GTTACTAGCA CTTTTGGAAA TGGAGATTCT CCAGGTAACG GAAAGACCTT GAAGGACTCT 
TTGCTCAGAC TAAAACTGCT GAGAAATAAA ATTAGGTATG CTGTGTTTGG TCTGGGGTCC 
AGCATGTACC CAGAGTTCTG TGCCTTTGCT CATGCCATTG ACCAAAAACT GGCCCAACTC 
GGGGCTTCCC AGCTCACTCC AGTGGGTGAA GGGGATGAAC TCAATGGGCA AGAAGAAGCT 
TTCCGCAGCT GGGCAGTCAG TGCTTTCAAG GCTGCCTGTG ACATCTTTAA CGTCCGTGGG 
AGACACAGTA TTCAGTTGCC TAAGATCTAC ACCTCTGAGG AAAGGTGGGA CCCTACCAAT 
TACAGGCTAG TGCATGACTC CCAGCCCATG GACCTGGCTA AAGCTCTTGC AAACATTCAT 
GCCAAGGATG TAATTCCCAT GAAGCTGAAA TTTAGGCAGA ATCTTCAAAG TTTGAAGTCC 
AGTCGTGTCA CCATTCTGGT TAAACTTCAG TGTGAGACAG ATCAGGAAGT GCACTACCTT 
CCCGGGGAGC ACATCGGGAT TTTCCCAGGC AACCAAGCAG AATTAGTCCA TGGCATTATT 
GCACATGTCA AGGATGCCCC TCCAGCTGAT CAGACTATCA GGCTTGAAAC CTGCATTGAT 
GGTGGGTACT GGGCAAGTCA CAAAAAGATT CCAGCCTGCA CACTACCTGA GGCTTTGACA 
TACTTGCTTG ATATCACTAC TCCACCCTCC CAACAACTGC TAAAAAAACT CTCCCAGCTG 
GTGACAGCAG AAGGAGATAA GCAAAGGCTG GAGGTGCTGT GTCAGAGCAC AGAAGAATAC 
AATAAATGGA AGTTTTACAA CAGCCCAAAC ATCCTGGAGG TGCTGGAGGA GTTCCCCTCT 
GCTGAGGTCT CTGCAGCTTT CCTGCTGACT CAGCTGCCAC TTCTGAAGCC CAGGTACTAT 
TCTGTCAGCT CCTCCTGTGA CCTGACAGCC AGAGAGATTC ATCTGACAGT TGCAGTTGTA 
AACTACAGGA CAAGAGATGG TGAAGGGCCT TTGCACCATG GAGTCTGCAG CACCTGGCTG 
AACACAATAG AGCTCAATGA AACCGTTCCC TGCTTCATCC GCAGTGCTAA TGAGTTCCAC 
CTCCCGGAGG AGCCATCCAA GCCCTGCATT CTGATCGGCT CAGGCACGGG GATTGCTCCC 
TTCAGGAGCT TCTGGCTGCA GCGCCTCTAT GACCTGGAGC ACAGAGGGCT CAGAGGAGGG 
AACATGACCC TGCTGTTCGG CTGCCGGCAT CCAGACCTGG ATCACATCTA CAGGGAAGAG 
ACTGAGGAGA TGAAGAGGAA GGGAGTCCTG AGTGATGTCT ACACAGCTTA CTCCAGACTG 
CCTGGCCAAG AGAAAGTGTA CGTCCAGGAC GTGCTGCAGG GGCAGCTGGC GGCCCGCGTG 
TGCGAGGTGC TGCAGGGGCA GCAGGGCCAT GTGTACGTGT GCGGGGACGT GCGCATGGCG 
CGGGACGTGG CCGGGGCGCT GCGGGCGCTG CTCGCCCGCG CCCTGCGCCT CAGCCAGCAG 
CAGGCCAGCG AGTTCCTGCA GCAGCTCAAG AGCCAAAAGC GATACCATGA AGATATATTT 
GGGGCTGTGT TCGCACATGA AGTCAAAAGA GCTTTGCAAC CCACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030143830.1
CDS	6623..10093
Translation	MLCPWQFVFKSRAAKTQYPQEKDINNNVEKNRKIHGSEKDDAKLHNPSKKQMERLPIITSAKPSEGGEVCSQHHGLMNLPSNDIKLSNQVSGCPRHVRVRNLENGSSFLDTLHLTAKEVINCQTKACQGSLMTPKGLVRGTRDGPVPLQDLLPQAIDFLKQYYSSFKEPKIKEELGRLEAVTKEIETTGTYHLTKDELAFAAKQAWRNAPRCIGRIQWSNLQVFDARDCKTAKEMFEHICHHIQYATNNGNIRSAITVFPHRTDGQHDFRVWNSQLIRYAGYQMSDGSVLGDPAAVEFTQLCIELGWKPKYGRFDVLPLVLQANGQDPEIFELPPEIVLQVPMEHPKYKWFKELELKWYALPAVANMLLEAGGLEFPACPFNGWYMGTEIGVRDFCDAQRYNILKEVGRRMGLETNKLSSLWKDRAVVEINVAVLHSFQRQNVTIMDHHSASESFLKYMQSEYRARGGCPADWVWIVPPMSGSITPVFHQEMLNYVLTPFYYYQVDAWKTHVWHDETRRRKKKEIKFSILAKAVLFASSLMRGAVATRAKVTVIYATETGKSETLANSLCSLFSCAFSTKILCMDEYNICDLEKETLLLVVTSTFGNGDSPGNGKTLKDSLLRLKLLRNKIRYAVFGLGSSMYPEFCAFAHAIDQKLAQLGASQLTPVGEGDELNGQEEAFRSWAVSAFKAACDIFNVRGRHSIQLPKIYTSEERWDPTNYRLVHDSQPMDLAKALANIHAKDVIPMKLKFRQNLQSLKSSRVTILVKLQCETDQEVHYLPGEHIGIFPGNQAELVHGIIAHVKDAPPADQTIRLETCIDGGYWASHKKIPACTLPEALTYLLDITTPPSQQLLKKLSQLVTAEGDKQRLEVLCQSTEEYNKWKFYNSPNILEVLEEFPSAEVSAAFLLTQLPLLKPRYYSVSSSCDLTAREIHLTVAVVNYRTRDGEGPLHHGVCSTWLNTIELNETVPCFIRSANEFHLPEEPSKPCILIGSGTGIAPFRSFWLQRLYDLEHRGLRGGNMTLLFGCRHPDLDHIYREETEEMKRKGVLSDVYTAYSRLPGQEKVYVQDVLQGQLAARVCEVLQGQQGHVYVCGDVRMARDVAGALRALLARALRLSQQQASEFLQQLKSQKRYHEDIFGAVFAHEVKRALQPTS

Target ORF information:

RefSeq Version	XM_030287970.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030287970.1

ORF Insert Sequence:

ATGCTGTGCC CATGGCAGTT TGTGTTCAAA TCTCGTGCTG CCAAGACCCA GTACCCCCAG 
GAGAAGGATA TCAACAACAA CGTGGAGAAA AATAGGAAGA TCCATGGCTC AGAAAAGGAT 
GATGCCAAGT TGCACAATCC GAGCAAGAAG CAGATGGAGA GGCTGCCTAT CATAACTTCA 
GCAAAGCCCA GTGAGGGAGG AGAGGTCTGT TCCCAGCATC ATGGTTTGAT GAATCTCCCT 
TCAAATGACA TCAAACTTTC AAACCAAGTA TCAGGATGTC CAAGACATGT AAGAGTAAGA 
AACTTGGAAA ATGGATCCAG CTTTCTTGAC ACACTACACC TGACTGCAAA AGAGGTTATC 
AATTGCCAGA CCAAAGCCTG CCAAGGGTCA CTCATGACCC CAAAGGGCCT GGTGAGAGGT 
ACCAGAGATG GGCCGGTTCC ACTACAGGAT CTTTTACCTC AGGCAATAGA CTTCCTCAAG 
CAATACTACA GTTCATTTAA AGAGCCAAAA ATCAAAGAAG AGCTGGGCCG GCTGGAAGCA 
GTGACCAAGG AGATAGAAAC AACAGGAACT TACCACCTGA CAAAGGATGA GTTGGCCTTT 
GCTGCCAAGC AGGCCTGGAG GAATGCCCCC AGGTGCATTG GGAGGATCCA GTGGTCCAAC 
CTGCAGGTGT TTGATGCACG AGACTGTAAA ACAGCCAAGG AAATGTTTGA GCATATCTGT 
CATCATATTC AGTATGCCAC AAACAATGGT AATATAAGGT CAGCCATCAC GGTGTTCCCG 
CACAGGACGG ACGGGCAGCA CGATTTCCGT GTGTGGAACA GCCAGCTGAT CCGCTACGCT 
GGCTACCAAA TGTCAGATGG CTCTGTCCTT GGAGACCCTG CTGCTGTGGA GTTCACTCAG 
TTGTGCATTG AACTTGGGTG GAAGCCGAAA TATGGCCGCT TTGATGTACT TCCACTTGTT 
CTCCAAGCAA ATGGCCAAGA CCCAGAAATA TTTGAATTAC CTCCAGAAAT TGTCCTCCAA 
GTGCCAATGG AGCATCCAAA GTACAAGTGG TTTAAGGAGC TGGAGCTGAA GTGGTACGCG 
CTGCCTGCTG TGGCCAACAT GCTTCTGGAG GCAGGAGGGC TGGAGTTCCC CGCATGCCCT 
TTCAATGGCT GGTACATGGG GACAGAGATC GGCGTGCGGG ACTTCTGCGA CGCGCAGCGC 
TACAACATCC TGAAGGAGGT AGGGAGGAGA ATGGGACTGG AAACAAATAA ACTGTCATCA 
TTATGGAAGG ACCGAGCTGT TGTAGAGATC AATGTGGCTG TGCTTCACAG CTTCCAGAGA 
CAGAATGTAA CCATCATGGA TCACCACTCA GCCTCCGAGT CCTTCCTGAA GTACATGCAG 
AGCGAGTACC GCGCGCGGGG CGGCTGCCCG GCAGATTGGG TGTGGATCGT GCCTCCCATG 
TCAGGCAGCA TAACCCCCGT GTTCCACCAA GAGATGTTGA ACTATGTCCT CACTCCCTTC 
TATTACTACC AGGTAGATGC ATGGAAAACA CATGTATGGC ATGATGAGAC CCGAAGGCGA 
AAGAAAAAAG AAATAAAGTT TAGCATCTTG GCAAAGGCTG TGCTCTTTGC ATCCTCACTG 
ATGCGAGGGG CAGTGGCCAC CAGGGCCAAG GTCACCGTGA TCTACGCGAC AGAAACGGGC 
AAGTCGGAGA CGCTCGCCAA CAGCCTGTGC AGCCTGTTCA GCTGTGCCTT CAGCACCAAG 
ATCCTCTGCA TGGATGAGTA CAATATTTGT GACCTGGAAA AAGAAACACT TCTTTTAGTG 
GTTACTAGCA CTTTTGGAAA TGGAGATTCT CCAGGTAACG GAAAGACCTT GAAGGACTCT 
TTGCTCAGAC TAAAACTGCT GAGAAATAAA ATTAGGTATG CTGTGTTTGG TCTGGGGTCC 
AGCATGTACC CAGAGTTCTG TGCCTTTGCT CATGCCATTG ACCAAAAACT GGCCCAACTC 
GGGGCTTCCC AGCTCACTCC AGTGGGTGAA GGGGATGAAC TCAATGGGCA AGAAGAAGCT 
TTCCGCAGCT GGGCAGTCAG TGCTTTCAAG GCTGCCTGTG ACATCTTTAA CGTCCGTGGG 
AGACACAGTA TTCAGTTGCC TAAGATCTAC ACCTCTGAGG AAAGGTGGGA CCCTACCAAT 
TACAGGCTAG TGCATGACTC CCAGCCCATG GACCTGGCTA AAGCTCTTGC AAACATTCAT 
GCCAAGGATG TAATTCCCAT GAAGCTGAAA TTTAGGCAGA ATCTTCAAAG TTTGAAGTCC 
AGTCGTGTCA CCATTCTGGT TAAACTTCAG TGTGAGACAG ATCAGGAAGT GCACTACCTT 
CCCGGGGAGC ACATCGGGAT TTTCCCAGGC AACCAAGCAG AATTAGTCCA TGGCATTATT 
GCACATGTCA AGGATGCCCC TCCAGCTGAT CAGACTATCA GGCTTGAAAC CTGCATTGAT 
GGTGGGTACT GGGCAAGTCA CAAAAAGATT CCAGCCTGCA CACTACCTGA GGCTTTGACA 
TACTTGCTTG ATATCACTAC TCCACCCTCC CAACAACTGC TAAAAAAACT CTCCCAGCTG 
GTGACAGCAG AAGGAGATAA GCAAAGGCTG GAGGTGCTGT GTCAGAGCAC AGAAGAATAC 
AATAAATGGA AGTTTTACAA CAGCCCAAAC ATCCTGGAGG TGCTGGAGGA GTTCCCCTCT 
GCTGAGGTCT CTGCAGCTTT CCTGCTGACT CAGCTGCCAC TTCTGAAGCC CAGGTACTAT 
TCTGTCAGCT CCTCCTGTGA CCTGACAGCC AGAGAGATTC ATCTGACAGT TGCAGTTGTA 
AACTACAGGA CAAGAGATGG TGAAGGGCCT TTGCACCATG GAGTCTGCAG CACCTGGCTG 
AACACAATAG AGCTCAATGA AACCGTTCCC TGCTTCATCC GCAGTGCTAA TGAGTTCCAC 
CTCCCGGAGG AGCCATCCAA GCCCTGCATT CTGATCGGCT CAGGCACGGG GATTGCTCCC 
TTCAGGAGCT TCTGGCTGCA GCGCCTCTAT GACCTGGAGC ACAGAGGGCT CAGAGGAGGG 
AACATGACCC TGCTGTTCGG CTGCCGGCAT CCAGACCTGG ATCACATCTA CAGGGAAGAG 
ACTGAGGAGA TGAAGAGGAA GGGAGTCCTG AGTGATGTCT ACACAGCTTA CTCCAGACTG 
CCTGGCCAAG AGAAAGTGTA CGTCCAGGAC GTGCTGCAGG GGCAGCTGGC GGCCCGCGTG 
TGCGAGGTGC TGCAGGGGCA GCAGGGCCAT GTGTACGTGT GCGGGGACGT GCGCATGGCG 
CGGGACGTGG CCGGGGCGCT GCGGGCGCTG CTCGCCCGCG CCCTGCGCCT CAGCCAGCAG 
CAGGCCAGCG AGTTCCTGCA GCAGCTCAAG AGCCAAAAGC GATACCATGA AGATATATTT 
GGGGCTGTGT TCGCACATGA AGTCAAAAGA GCTTTGCAAC CCACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_030287971.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030143831.1 Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

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CloneID	OTd31999
Clone ID Related Accession (Same CDS sequence)	XM_030287970.1 , XM_030287971.1 , XM_030287973.1
Accession Version	XM_030287971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	nitric oxide synthase, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCC CATGGCAGTT TGTGTTCAAA TCTCGTGCTG CCAAGACCCA GTACCCCCAG 
GAGAAGGATA TCAACAACAA CGTGGAGAAA AATAGGAAGA TCCATGGCTC AGAAAAGGAT 
GATGCCAAGT TGCACAATCC GAGCAAGAAG CAGATGGAGA GGCTGCCTAT CATAACTTCA 
GCAAAGCCCA GTGAGGGAGG AGAGGTCTGT TCCCAGCATC ATGGTTTGAT GAATCTCCCT 
TCAAATGACA TCAAACTTTC AAACCAAGTA TCAGGATGTC CAAGACATGT AAGAGTAAGA 
AACTTGGAAA ATGGATCCAG CTTTCTTGAC ACACTACACC TGACTGCAAA AGAGGTTATC 
AATTGCCAGA CCAAAGCCTG CCAAGGGTCA CTCATGACCC CAAAGGGCCT GGTGAGAGGT 
ACCAGAGATG GGCCGGTTCC ACTACAGGAT CTTTTACCTC AGGCAATAGA CTTCCTCAAG 
CAATACTACA GTTCATTTAA AGAGCCAAAA ATCAAAGAAG AGCTGGGCCG GCTGGAAGCA 
GTGACCAAGG AGATAGAAAC AACAGGAACT TACCACCTGA CAAAGGATGA GTTGGCCTTT 
GCTGCCAAGC AGGCCTGGAG GAATGCCCCC AGGTGCATTG GGAGGATCCA GTGGTCCAAC 
CTGCAGGTGT TTGATGCACG AGACTGTAAA ACAGCCAAGG AAATGTTTGA GCATATCTGT 
CATCATATTC AGTATGCCAC AAACAATGGT AATATAAGGT CAGCCATCAC GGTGTTCCCG 
CACAGGACGG ACGGGCAGCA CGATTTCCGT GTGTGGAACA GCCAGCTGAT CCGCTACGCT 
GGCTACCAAA TGTCAGATGG CTCTGTCCTT GGAGACCCTG CTGCTGTGGA GTTCACTCAG 
TTGTGCATTG AACTTGGGTG GAAGCCGAAA TATGGCCGCT TTGATGTACT TCCACTTGTT 
CTCCAAGCAA ATGGCCAAGA CCCAGAAATA TTTGAATTAC CTCCAGAAAT TGTCCTCCAA 
GTGCCAATGG AGCATCCAAA GTACAAGTGG TTTAAGGAGC TGGAGCTGAA GTGGTACGCG 
CTGCCTGCTG TGGCCAACAT GCTTCTGGAG GCAGGAGGGC TGGAGTTCCC CGCATGCCCT 
TTCAATGGCT GGTACATGGG GACAGAGATC GGCGTGCGGG ACTTCTGCGA CGCGCAGCGC 
TACAACATCC TGAAGGAGGT AGGGAGGAGA ATGGGACTGG AAACAAATAA ACTGTCATCA 
TTATGGAAGG ACCGAGCTGT TGTAGAGATC AATGTGGCTG TGCTTCACAG CTTCCAGAGA 
CAGAATGTAA CCATCATGGA TCACCACTCA GCCTCCGAGT CCTTCCTGAA GTACATGCAG 
AGCGAGTACC GCGCGCGGGG CGGCTGCCCG GCAGATTGGG TGTGGATCGT GCCTCCCATG 
TCAGGCAGCA TAACCCCCGT GTTCCACCAA GAGATGTTGA ACTATGTCCT CACTCCCTTC 
TATTACTACC AGGTAGATGC ATGGAAAACA CATGTATGGC ATGATGAGAC CCGAAGGCGA 
AAGAAAAAAG AAATAAAGTT TAGCATCTTG GCAAAGGCTG TGCTCTTTGC ATCCTCACTG 
ATGCGAGGGG CAGTGGCCAC CAGGGCCAAG GTCACCGTGA TCTACGCGAC AGAAACGGGC 
AAGTCGGAGA CGCTCGCCAA CAGCCTGTGC AGCCTGTTCA GCTGTGCCTT CAGCACCAAG 
ATCCTCTGCA TGGATGAGTA CAATATTTGT GACCTGGAAA AAGAAACACT TCTTTTAGTG 
GTTACTAGCA CTTTTGGAAA TGGAGATTCT CCAGGTAACG GAAAGACCTT GAAGGACTCT 
TTGCTCAGAC TAAAACTGCT GAGAAATAAA ATTAGGTATG CTGTGTTTGG TCTGGGGTCC 
AGCATGTACC CAGAGTTCTG TGCCTTTGCT CATGCCATTG ACCAAAAACT GGCCCAACTC 
GGGGCTTCCC AGCTCACTCC AGTGGGTGAA GGGGATGAAC TCAATGGGCA AGAAGAAGCT 
TTCCGCAGCT GGGCAGTCAG TGCTTTCAAG GCTGCCTGTG ACATCTTTAA CGTCCGTGGG 
AGACACAGTA TTCAGTTGCC TAAGATCTAC ACCTCTGAGG AAAGGTGGGA CCCTACCAAT 
TACAGGCTAG TGCATGACTC CCAGCCCATG GACCTGGCTA AAGCTCTTGC AAACATTCAT 
GCCAAGGATG TAATTCCCAT GAAGCTGAAA TTTAGGCAGA ATCTTCAAAG TTTGAAGTCC 
AGTCGTGTCA CCATTCTGGT TAAACTTCAG TGTGAGACAG ATCAGGAAGT GCACTACCTT 
CCCGGGGAGC ACATCGGGAT TTTCCCAGGC AACCAAGCAG AATTAGTCCA TGGCATTATT 
GCACATGTCA AGGATGCCCC TCCAGCTGAT CAGACTATCA GGCTTGAAAC CTGCATTGAT 
GGTGGGTACT GGGCAAGTCA CAAAAAGATT CCAGCCTGCA CACTACCTGA GGCTTTGACA 
TACTTGCTTG ATATCACTAC TCCACCCTCC CAACAACTGC TAAAAAAACT CTCCCAGCTG 
GTGACAGCAG AAGGAGATAA GCAAAGGCTG GAGGTGCTGT GTCAGAGCAC AGAAGAATAC 
AATAAATGGA AGTTTTACAA CAGCCCAAAC ATCCTGGAGG TGCTGGAGGA GTTCCCCTCT 
GCTGAGGTCT CTGCAGCTTT CCTGCTGACT CAGCTGCCAC TTCTGAAGCC CAGGTACTAT 
TCTGTCAGCT CCTCCTGTGA CCTGACAGCC AGAGAGATTC ATCTGACAGT TGCAGTTGTA 
AACTACAGGA CAAGAGATGG TGAAGGGCCT TTGCACCATG GAGTCTGCAG CACCTGGCTG 
AACACAATAG AGCTCAATGA AACCGTTCCC TGCTTCATCC GCAGTGCTAA TGAGTTCCAC 
CTCCCGGAGG AGCCATCCAA GCCCTGCATT CTGATCGGCT CAGGCACGGG GATTGCTCCC 
TTCAGGAGCT TCTGGCTGCA GCGCCTCTAT GACCTGGAGC ACAGAGGGCT CAGAGGAGGG 
AACATGACCC TGCTGTTCGG CTGCCGGCAT CCAGACCTGG ATCACATCTA CAGGGAAGAG 
ACTGAGGAGA TGAAGAGGAA GGGAGTCCTG AGTGATGTCT ACACAGCTTA CTCCAGACTG 
CCTGGCCAAG AGAAAGTGTA CGTCCAGGAC GTGCTGCAGG GGCAGCTGGC GGCCCGCGTG 
TGCGAGGTGC TGCAGGGGCA GCAGGGCCAT GTGTACGTGT GCGGGGACGT GCGCATGGCG 
CGGGACGTGG CCGGGGCGCT GCGGGCGCTG CTCGCCCGCG CCCTGCGCCT CAGCCAGCAG 
CAGGCCAGCG AGTTCCTGCA GCAGCTCAAG AGCCAAAAGC GATACCATGA AGATATATTT 
GGGGCTGTGT TCGCACATGA AGTCAAAAGA GCTTTGCAAC CCACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030143831.1
CDS	121..3591
Translation	MLCPWQFVFKSRAAKTQYPQEKDINNNVEKNRKIHGSEKDDAKLHNPSKKQMERLPIITSAKPSEGGEVCSQHHGLMNLPSNDIKLSNQVSGCPRHVRVRNLENGSSFLDTLHLTAKEVINCQTKACQGSLMTPKGLVRGTRDGPVPLQDLLPQAIDFLKQYYSSFKEPKIKEELGRLEAVTKEIETTGTYHLTKDELAFAAKQAWRNAPRCIGRIQWSNLQVFDARDCKTAKEMFEHICHHIQYATNNGNIRSAITVFPHRTDGQHDFRVWNSQLIRYAGYQMSDGSVLGDPAAVEFTQLCIELGWKPKYGRFDVLPLVLQANGQDPEIFELPPEIVLQVPMEHPKYKWFKELELKWYALPAVANMLLEAGGLEFPACPFNGWYMGTEIGVRDFCDAQRYNILKEVGRRMGLETNKLSSLWKDRAVVEINVAVLHSFQRQNVTIMDHHSASESFLKYMQSEYRARGGCPADWVWIVPPMSGSITPVFHQEMLNYVLTPFYYYQVDAWKTHVWHDETRRRKKKEIKFSILAKAVLFASSLMRGAVATRAKVTVIYATETGKSETLANSLCSLFSCAFSTKILCMDEYNICDLEKETLLLVVTSTFGNGDSPGNGKTLKDSLLRLKLLRNKIRYAVFGLGSSMYPEFCAFAHAIDQKLAQLGASQLTPVGEGDELNGQEEAFRSWAVSAFKAACDIFNVRGRHSIQLPKIYTSEERWDPTNYRLVHDSQPMDLAKALANIHAKDVIPMKLKFRQNLQSLKSSRVTILVKLQCETDQEVHYLPGEHIGIFPGNQAELVHGIIAHVKDAPPADQTIRLETCIDGGYWASHKKIPACTLPEALTYLLDITTPPSQQLLKKLSQLVTAEGDKQRLEVLCQSTEEYNKWKFYNSPNILEVLEEFPSAEVSAAFLLTQLPLLKPRYYSVSSSCDLTAREIHLTVAVVNYRTRDGEGPLHHGVCSTWLNTIELNETVPCFIRSANEFHLPEEPSKPCILIGSGTGIAPFRSFWLQRLYDLEHRGLRGGNMTLLFGCRHPDLDHIYREETEEMKRKGVLSDVYTAYSRLPGQEKVYVQDVLQGQLAARVCEVLQGQQGHVYVCGDVRMARDVAGALRALLARALRLSQQQASEFLQQLKSQKRYHEDIFGAVFAHEVKRALQPTS

Target ORF information:

RefSeq Version	XM_030287971.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030287971.1

ORF Insert Sequence:

ATGCTGTGCC CATGGCAGTT TGTGTTCAAA TCTCGTGCTG CCAAGACCCA GTACCCCCAG 
GAGAAGGATA TCAACAACAA CGTGGAGAAA AATAGGAAGA TCCATGGCTC AGAAAAGGAT 
GATGCCAAGT TGCACAATCC GAGCAAGAAG CAGATGGAGA GGCTGCCTAT CATAACTTCA 
GCAAAGCCCA GTGAGGGAGG AGAGGTCTGT TCCCAGCATC ATGGTTTGAT GAATCTCCCT 
TCAAATGACA TCAAACTTTC AAACCAAGTA TCAGGATGTC CAAGACATGT AAGAGTAAGA 
AACTTGGAAA ATGGATCCAG CTTTCTTGAC ACACTACACC TGACTGCAAA AGAGGTTATC 
AATTGCCAGA CCAAAGCCTG CCAAGGGTCA CTCATGACCC CAAAGGGCCT GGTGAGAGGT 
ACCAGAGATG GGCCGGTTCC ACTACAGGAT CTTTTACCTC AGGCAATAGA CTTCCTCAAG 
CAATACTACA GTTCATTTAA AGAGCCAAAA ATCAAAGAAG AGCTGGGCCG GCTGGAAGCA 
GTGACCAAGG AGATAGAAAC AACAGGAACT TACCACCTGA CAAAGGATGA GTTGGCCTTT 
GCTGCCAAGC AGGCCTGGAG GAATGCCCCC AGGTGCATTG GGAGGATCCA GTGGTCCAAC 
CTGCAGGTGT TTGATGCACG AGACTGTAAA ACAGCCAAGG AAATGTTTGA GCATATCTGT 
CATCATATTC AGTATGCCAC AAACAATGGT AATATAAGGT CAGCCATCAC GGTGTTCCCG 
CACAGGACGG ACGGGCAGCA CGATTTCCGT GTGTGGAACA GCCAGCTGAT CCGCTACGCT 
GGCTACCAAA TGTCAGATGG CTCTGTCCTT GGAGACCCTG CTGCTGTGGA GTTCACTCAG 
TTGTGCATTG AACTTGGGTG GAAGCCGAAA TATGGCCGCT TTGATGTACT TCCACTTGTT 
CTCCAAGCAA ATGGCCAAGA CCCAGAAATA TTTGAATTAC CTCCAGAAAT TGTCCTCCAA 
GTGCCAATGG AGCATCCAAA GTACAAGTGG TTTAAGGAGC TGGAGCTGAA GTGGTACGCG 
CTGCCTGCTG TGGCCAACAT GCTTCTGGAG GCAGGAGGGC TGGAGTTCCC CGCATGCCCT 
TTCAATGGCT GGTACATGGG GACAGAGATC GGCGTGCGGG ACTTCTGCGA CGCGCAGCGC 
TACAACATCC TGAAGGAGGT AGGGAGGAGA ATGGGACTGG AAACAAATAA ACTGTCATCA 
TTATGGAAGG ACCGAGCTGT TGTAGAGATC AATGTGGCTG TGCTTCACAG CTTCCAGAGA 
CAGAATGTAA CCATCATGGA TCACCACTCA GCCTCCGAGT CCTTCCTGAA GTACATGCAG 
AGCGAGTACC GCGCGCGGGG CGGCTGCCCG GCAGATTGGG TGTGGATCGT GCCTCCCATG 
TCAGGCAGCA TAACCCCCGT GTTCCACCAA GAGATGTTGA ACTATGTCCT CACTCCCTTC 
TATTACTACC AGGTAGATGC ATGGAAAACA CATGTATGGC ATGATGAGAC CCGAAGGCGA 
AAGAAAAAAG AAATAAAGTT TAGCATCTTG GCAAAGGCTG TGCTCTTTGC ATCCTCACTG 
ATGCGAGGGG CAGTGGCCAC CAGGGCCAAG GTCACCGTGA TCTACGCGAC AGAAACGGGC 
AAGTCGGAGA CGCTCGCCAA CAGCCTGTGC AGCCTGTTCA GCTGTGCCTT CAGCACCAAG 
ATCCTCTGCA TGGATGAGTA CAATATTTGT GACCTGGAAA AAGAAACACT TCTTTTAGTG 
GTTACTAGCA CTTTTGGAAA TGGAGATTCT CCAGGTAACG GAAAGACCTT GAAGGACTCT 
TTGCTCAGAC TAAAACTGCT GAGAAATAAA ATTAGGTATG CTGTGTTTGG TCTGGGGTCC 
AGCATGTACC CAGAGTTCTG TGCCTTTGCT CATGCCATTG ACCAAAAACT GGCCCAACTC 
GGGGCTTCCC AGCTCACTCC AGTGGGTGAA GGGGATGAAC TCAATGGGCA AGAAGAAGCT 
TTCCGCAGCT GGGCAGTCAG TGCTTTCAAG GCTGCCTGTG ACATCTTTAA CGTCCGTGGG 
AGACACAGTA TTCAGTTGCC TAAGATCTAC ACCTCTGAGG AAAGGTGGGA CCCTACCAAT 
TACAGGCTAG TGCATGACTC CCAGCCCATG GACCTGGCTA AAGCTCTTGC AAACATTCAT 
GCCAAGGATG TAATTCCCAT GAAGCTGAAA TTTAGGCAGA ATCTTCAAAG TTTGAAGTCC 
AGTCGTGTCA CCATTCTGGT TAAACTTCAG TGTGAGACAG ATCAGGAAGT GCACTACCTT 
CCCGGGGAGC ACATCGGGAT TTTCCCAGGC AACCAAGCAG AATTAGTCCA TGGCATTATT 
GCACATGTCA AGGATGCCCC TCCAGCTGAT CAGACTATCA GGCTTGAAAC CTGCATTGAT 
GGTGGGTACT GGGCAAGTCA CAAAAAGATT CCAGCCTGCA CACTACCTGA GGCTTTGACA 
TACTTGCTTG ATATCACTAC TCCACCCTCC CAACAACTGC TAAAAAAACT CTCCCAGCTG 
GTGACAGCAG AAGGAGATAA GCAAAGGCTG GAGGTGCTGT GTCAGAGCAC AGAAGAATAC 
AATAAATGGA AGTTTTACAA CAGCCCAAAC ATCCTGGAGG TGCTGGAGGA GTTCCCCTCT 
GCTGAGGTCT CTGCAGCTTT CCTGCTGACT CAGCTGCCAC TTCTGAAGCC CAGGTACTAT 
TCTGTCAGCT CCTCCTGTGA CCTGACAGCC AGAGAGATTC ATCTGACAGT TGCAGTTGTA 
AACTACAGGA CAAGAGATGG TGAAGGGCCT TTGCACCATG GAGTCTGCAG CACCTGGCTG 
AACACAATAG AGCTCAATGA AACCGTTCCC TGCTTCATCC GCAGTGCTAA TGAGTTCCAC 
CTCCCGGAGG AGCCATCCAA GCCCTGCATT CTGATCGGCT CAGGCACGGG GATTGCTCCC 
TTCAGGAGCT TCTGGCTGCA GCGCCTCTAT GACCTGGAGC ACAGAGGGCT CAGAGGAGGG 
AACATGACCC TGCTGTTCGG CTGCCGGCAT CCAGACCTGG ATCACATCTA CAGGGAAGAG 
ACTGAGGAGA TGAAGAGGAA GGGAGTCCTG AGTGATGTCT ACACAGCTTA CTCCAGACTG 
CCTGGCCAAG AGAAAGTGTA CGTCCAGGAC GTGCTGCAGG GGCAGCTGGC GGCCCGCGTG 
TGCGAGGTGC TGCAGGGGCA GCAGGGCCAT GTGTACGTGT GCGGGGACGT GCGCATGGCG 
CGGGACGTGG CCGGGGCGCT GCGGGCGCTG CTCGCCCGCG CCCTGCGCCT CAGCCAGCAG 
CAGGCCAGCG AGTTCCTGCA GCAGCTCAAG AGCCAAAAGC GATACCATGA AGATATATTT 
GGGGCTGTGT TCGCACATGA AGTCAAAAGA GCTTTGCAAC CCACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_030287973.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030143833.1 Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

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CloneID	OTd31999
Clone ID Related Accession (Same CDS sequence)	XM_030287970.1 , XM_030287971.1 , XM_030287973.1
Accession Version	XM_030287973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	nitric oxide synthase, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCC CATGGCAGTT TGTGTTCAAA TCTCGTGCTG CCAAGACCCA GTACCCCCAG 
GAGAAGGATA TCAACAACAA CGTGGAGAAA AATAGGAAGA TCCATGGCTC AGAAAAGGAT 
GATGCCAAGT TGCACAATCC GAGCAAGAAG CAGATGGAGA GGCTGCCTAT CATAACTTCA 
GCAAAGCCCA GTGAGGGAGG AGAGGTCTGT TCCCAGCATC ATGGTTTGAT GAATCTCCCT 
TCAAATGACA TCAAACTTTC AAACCAAGTA TCAGGATGTC CAAGACATGT AAGAGTAAGA 
AACTTGGAAA ATGGATCCAG CTTTCTTGAC ACACTACACC TGACTGCAAA AGAGGTTATC 
AATTGCCAGA CCAAAGCCTG CCAAGGGTCA CTCATGACCC CAAAGGGCCT GGTGAGAGGT 
ACCAGAGATG GGCCGGTTCC ACTACAGGAT CTTTTACCTC AGGCAATAGA CTTCCTCAAG 
CAATACTACA GTTCATTTAA AGAGCCAAAA ATCAAAGAAG AGCTGGGCCG GCTGGAAGCA 
GTGACCAAGG AGATAGAAAC AACAGGAACT TACCACCTGA CAAAGGATGA GTTGGCCTTT 
GCTGCCAAGC AGGCCTGGAG GAATGCCCCC AGGTGCATTG GGAGGATCCA GTGGTCCAAC 
CTGCAGGTGT TTGATGCACG AGACTGTAAA ACAGCCAAGG AAATGTTTGA GCATATCTGT 
CATCATATTC AGTATGCCAC AAACAATGGT AATATAAGGT CAGCCATCAC GGTGTTCCCG 
CACAGGACGG ACGGGCAGCA CGATTTCCGT GTGTGGAACA GCCAGCTGAT CCGCTACGCT 
GGCTACCAAA TGTCAGATGG CTCTGTCCTT GGAGACCCTG CTGCTGTGGA GTTCACTCAG 
TTGTGCATTG AACTTGGGTG GAAGCCGAAA TATGGCCGCT TTGATGTACT TCCACTTGTT 
CTCCAAGCAA ATGGCCAAGA CCCAGAAATA TTTGAATTAC CTCCAGAAAT TGTCCTCCAA 
GTGCCAATGG AGCATCCAAA GTACAAGTGG TTTAAGGAGC TGGAGCTGAA GTGGTACGCG 
CTGCCTGCTG TGGCCAACAT GCTTCTGGAG GCAGGAGGGC TGGAGTTCCC CGCATGCCCT 
TTCAATGGCT GGTACATGGG GACAGAGATC GGCGTGCGGG ACTTCTGCGA CGCGCAGCGC 
TACAACATCC TGAAGGAGGT AGGGAGGAGA ATGGGACTGG AAACAAATAA ACTGTCATCA 
TTATGGAAGG ACCGAGCTGT TGTAGAGATC AATGTGGCTG TGCTTCACAG CTTCCAGAGA 
CAGAATGTAA CCATCATGGA TCACCACTCA GCCTCCGAGT CCTTCCTGAA GTACATGCAG 
AGCGAGTACC GCGCGCGGGG CGGCTGCCCG GCAGATTGGG TGTGGATCGT GCCTCCCATG 
TCAGGCAGCA TAACCCCCGT GTTCCACCAA GAGATGTTGA ACTATGTCCT CACTCCCTTC 
TATTACTACC AGGTAGATGC ATGGAAAACA CATGTATGGC ATGATGAGAC CCGAAGGCGA 
AAGAAAAAAG AAATAAAGTT TAGCATCTTG GCAAAGGCTG TGCTCTTTGC ATCCTCACTG 
ATGCGAGGGG CAGTGGCCAC CAGGGCCAAG GTCACCGTGA TCTACGCGAC AGAAACGGGC 
AAGTCGGAGA CGCTCGCCAA CAGCCTGTGC AGCCTGTTCA GCTGTGCCTT CAGCACCAAG 
ATCCTCTGCA TGGATGAGTA CAATATTTGT GACCTGGAAA AAGAAACACT TCTTTTAGTG 
GTTACTAGCA CTTTTGGAAA TGGAGATTCT CCAGGTAACG GAAAGACCTT GAAGGACTCT 
TTGCTCAGAC TAAAACTGCT GAGAAATAAA ATTAGGTATG CTGTGTTTGG TCTGGGGTCC 
AGCATGTACC CAGAGTTCTG TGCCTTTGCT CATGCCATTG ACCAAAAACT GGCCCAACTC 
GGGGCTTCCC AGCTCACTCC AGTGGGTGAA GGGGATGAAC TCAATGGGCA AGAAGAAGCT 
TTCCGCAGCT GGGCAGTCAG TGCTTTCAAG GCTGCCTGTG ACATCTTTAA CGTCCGTGGG 
AGACACAGTA TTCAGTTGCC TAAGATCTAC ACCTCTGAGG AAAGGTGGGA CCCTACCAAT 
TACAGGCTAG TGCATGACTC CCAGCCCATG GACCTGGCTA AAGCTCTTGC AAACATTCAT 
GCCAAGGATG TAATTCCCAT GAAGCTGAAA TTTAGGCAGA ATCTTCAAAG TTTGAAGTCC 
AGTCGTGTCA CCATTCTGGT TAAACTTCAG TGTGAGACAG ATCAGGAAGT GCACTACCTT 
CCCGGGGAGC ACATCGGGAT TTTCCCAGGC AACCAAGCAG AATTAGTCCA TGGCATTATT 
GCACATGTCA AGGATGCCCC TCCAGCTGAT CAGACTATCA GGCTTGAAAC CTGCATTGAT 
GGTGGGTACT GGGCAAGTCA CAAAAAGATT CCAGCCTGCA CACTACCTGA GGCTTTGACA 
TACTTGCTTG ATATCACTAC TCCACCCTCC CAACAACTGC TAAAAAAACT CTCCCAGCTG 
GTGACAGCAG AAGGAGATAA GCAAAGGCTG GAGGTGCTGT GTCAGAGCAC AGAAGAATAC 
AATAAATGGA AGTTTTACAA CAGCCCAAAC ATCCTGGAGG TGCTGGAGGA GTTCCCCTCT 
GCTGAGGTCT CTGCAGCTTT CCTGCTGACT CAGCTGCCAC TTCTGAAGCC CAGGTACTAT 
TCTGTCAGCT CCTCCTGTGA CCTGACAGCC AGAGAGATTC ATCTGACAGT TGCAGTTGTA 
AACTACAGGA CAAGAGATGG TGAAGGGCCT TTGCACCATG GAGTCTGCAG CACCTGGCTG 
AACACAATAG AGCTCAATGA AACCGTTCCC TGCTTCATCC GCAGTGCTAA TGAGTTCCAC 
CTCCCGGAGG AGCCATCCAA GCCCTGCATT CTGATCGGCT CAGGCACGGG GATTGCTCCC 
TTCAGGAGCT TCTGGCTGCA GCGCCTCTAT GACCTGGAGC ACAGAGGGCT CAGAGGAGGG 
AACATGACCC TGCTGTTCGG CTGCCGGCAT CCAGACCTGG ATCACATCTA CAGGGAAGAG 
ACTGAGGAGA TGAAGAGGAA GGGAGTCCTG AGTGATGTCT ACACAGCTTA CTCCAGACTG 
CCTGGCCAAG AGAAAGTGTA CGTCCAGGAC GTGCTGCAGG GGCAGCTGGC GGCCCGCGTG 
TGCGAGGTGC TGCAGGGGCA GCAGGGCCAT GTGTACGTGT GCGGGGACGT GCGCATGGCG 
CGGGACGTGG CCGGGGCGCT GCGGGCGCTG CTCGCCCGCG CCCTGCGCCT CAGCCAGCAG 
CAGGCCAGCG AGTTCCTGCA GCAGCTCAAG AGCCAAAAGC GATACCATGA AGATATATTT 
GGGGCTGTGT TCGCACATGA AGTCAAAAGA GCTTTGCAAC CCACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030143833.1
CDS	1090..4560
Translation	MLCPWQFVFKSRAAKTQYPQEKDINNNVEKNRKIHGSEKDDAKLHNPSKKQMERLPIITSAKPSEGGEVCSQHHGLMNLPSNDIKLSNQVSGCPRHVRVRNLENGSSFLDTLHLTAKEVINCQTKACQGSLMTPKGLVRGTRDGPVPLQDLLPQAIDFLKQYYSSFKEPKIKEELGRLEAVTKEIETTGTYHLTKDELAFAAKQAWRNAPRCIGRIQWSNLQVFDARDCKTAKEMFEHICHHIQYATNNGNIRSAITVFPHRTDGQHDFRVWNSQLIRYAGYQMSDGSVLGDPAAVEFTQLCIELGWKPKYGRFDVLPLVLQANGQDPEIFELPPEIVLQVPMEHPKYKWFKELELKWYALPAVANMLLEAGGLEFPACPFNGWYMGTEIGVRDFCDAQRYNILKEVGRRMGLETNKLSSLWKDRAVVEINVAVLHSFQRQNVTIMDHHSASESFLKYMQSEYRARGGCPADWVWIVPPMSGSITPVFHQEMLNYVLTPFYYYQVDAWKTHVWHDETRRRKKKEIKFSILAKAVLFASSLMRGAVATRAKVTVIYATETGKSETLANSLCSLFSCAFSTKILCMDEYNICDLEKETLLLVVTSTFGNGDSPGNGKTLKDSLLRLKLLRNKIRYAVFGLGSSMYPEFCAFAHAIDQKLAQLGASQLTPVGEGDELNGQEEAFRSWAVSAFKAACDIFNVRGRHSIQLPKIYTSEERWDPTNYRLVHDSQPMDLAKALANIHAKDVIPMKLKFRQNLQSLKSSRVTILVKLQCETDQEVHYLPGEHIGIFPGNQAELVHGIIAHVKDAPPADQTIRLETCIDGGYWASHKKIPACTLPEALTYLLDITTPPSQQLLKKLSQLVTAEGDKQRLEVLCQSTEEYNKWKFYNSPNILEVLEEFPSAEVSAAFLLTQLPLLKPRYYSVSSSCDLTAREIHLTVAVVNYRTRDGEGPLHHGVCSTWLNTIELNETVPCFIRSANEFHLPEEPSKPCILIGSGTGIAPFRSFWLQRLYDLEHRGLRGGNMTLLFGCRHPDLDHIYREETEEMKRKGVLSDVYTAYSRLPGQEKVYVQDVLQGQLAARVCEVLQGQQGHVYVCGDVRMARDVAGALRALLARALRLSQQQASEFLQQLKSQKRYHEDIFGAVFAHEVKRALQPTS

Target ORF information:

RefSeq Version	XM_030287973.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030287973.1

ORF Insert Sequence:

ATGCTGTGCC CATGGCAGTT TGTGTTCAAA TCTCGTGCTG CCAAGACCCA GTACCCCCAG 
GAGAAGGATA TCAACAACAA CGTGGAGAAA AATAGGAAGA TCCATGGCTC AGAAAAGGAT 
GATGCCAAGT TGCACAATCC GAGCAAGAAG CAGATGGAGA GGCTGCCTAT CATAACTTCA 
GCAAAGCCCA GTGAGGGAGG AGAGGTCTGT TCCCAGCATC ATGGTTTGAT GAATCTCCCT 
TCAAATGACA TCAAACTTTC AAACCAAGTA TCAGGATGTC CAAGACATGT AAGAGTAAGA 
AACTTGGAAA ATGGATCCAG CTTTCTTGAC ACACTACACC TGACTGCAAA AGAGGTTATC 
AATTGCCAGA CCAAAGCCTG CCAAGGGTCA CTCATGACCC CAAAGGGCCT GGTGAGAGGT 
ACCAGAGATG GGCCGGTTCC ACTACAGGAT CTTTTACCTC AGGCAATAGA CTTCCTCAAG 
CAATACTACA GTTCATTTAA AGAGCCAAAA ATCAAAGAAG AGCTGGGCCG GCTGGAAGCA 
GTGACCAAGG AGATAGAAAC AACAGGAACT TACCACCTGA CAAAGGATGA GTTGGCCTTT 
GCTGCCAAGC AGGCCTGGAG GAATGCCCCC AGGTGCATTG GGAGGATCCA GTGGTCCAAC 
CTGCAGGTGT TTGATGCACG AGACTGTAAA ACAGCCAAGG AAATGTTTGA GCATATCTGT 
CATCATATTC AGTATGCCAC AAACAATGGT AATATAAGGT CAGCCATCAC GGTGTTCCCG 
CACAGGACGG ACGGGCAGCA CGATTTCCGT GTGTGGAACA GCCAGCTGAT CCGCTACGCT 
GGCTACCAAA TGTCAGATGG CTCTGTCCTT GGAGACCCTG CTGCTGTGGA GTTCACTCAG 
TTGTGCATTG AACTTGGGTG GAAGCCGAAA TATGGCCGCT TTGATGTACT TCCACTTGTT 
CTCCAAGCAA ATGGCCAAGA CCCAGAAATA TTTGAATTAC CTCCAGAAAT TGTCCTCCAA 
GTGCCAATGG AGCATCCAAA GTACAAGTGG TTTAAGGAGC TGGAGCTGAA GTGGTACGCG 
CTGCCTGCTG TGGCCAACAT GCTTCTGGAG GCAGGAGGGC TGGAGTTCCC CGCATGCCCT 
TTCAATGGCT GGTACATGGG GACAGAGATC GGCGTGCGGG ACTTCTGCGA CGCGCAGCGC 
TACAACATCC TGAAGGAGGT AGGGAGGAGA ATGGGACTGG AAACAAATAA ACTGTCATCA 
TTATGGAAGG ACCGAGCTGT TGTAGAGATC AATGTGGCTG TGCTTCACAG CTTCCAGAGA 
CAGAATGTAA CCATCATGGA TCACCACTCA GCCTCCGAGT CCTTCCTGAA GTACATGCAG 
AGCGAGTACC GCGCGCGGGG CGGCTGCCCG GCAGATTGGG TGTGGATCGT GCCTCCCATG 
TCAGGCAGCA TAACCCCCGT GTTCCACCAA GAGATGTTGA ACTATGTCCT CACTCCCTTC 
TATTACTACC AGGTAGATGC ATGGAAAACA CATGTATGGC ATGATGAGAC CCGAAGGCGA 
AAGAAAAAAG AAATAAAGTT TAGCATCTTG GCAAAGGCTG TGCTCTTTGC ATCCTCACTG 
ATGCGAGGGG CAGTGGCCAC CAGGGCCAAG GTCACCGTGA TCTACGCGAC AGAAACGGGC 
AAGTCGGAGA CGCTCGCCAA CAGCCTGTGC AGCCTGTTCA GCTGTGCCTT CAGCACCAAG 
ATCCTCTGCA TGGATGAGTA CAATATTTGT GACCTGGAAA AAGAAACACT TCTTTTAGTG 
GTTACTAGCA CTTTTGGAAA TGGAGATTCT CCAGGTAACG GAAAGACCTT GAAGGACTCT 
TTGCTCAGAC TAAAACTGCT GAGAAATAAA ATTAGGTATG CTGTGTTTGG TCTGGGGTCC 
AGCATGTACC CAGAGTTCTG TGCCTTTGCT CATGCCATTG ACCAAAAACT GGCCCAACTC 
GGGGCTTCCC AGCTCACTCC AGTGGGTGAA GGGGATGAAC TCAATGGGCA AGAAGAAGCT 
TTCCGCAGCT GGGCAGTCAG TGCTTTCAAG GCTGCCTGTG ACATCTTTAA CGTCCGTGGG 
AGACACAGTA TTCAGTTGCC TAAGATCTAC ACCTCTGAGG AAAGGTGGGA CCCTACCAAT 
TACAGGCTAG TGCATGACTC CCAGCCCATG GACCTGGCTA AAGCTCTTGC AAACATTCAT 
GCCAAGGATG TAATTCCCAT GAAGCTGAAA TTTAGGCAGA ATCTTCAAAG TTTGAAGTCC 
AGTCGTGTCA CCATTCTGGT TAAACTTCAG TGTGAGACAG ATCAGGAAGT GCACTACCTT 
CCCGGGGAGC ACATCGGGAT TTTCCCAGGC AACCAAGCAG AATTAGTCCA TGGCATTATT 
GCACATGTCA AGGATGCCCC TCCAGCTGAT CAGACTATCA GGCTTGAAAC CTGCATTGAT 
GGTGGGTACT GGGCAAGTCA CAAAAAGATT CCAGCCTGCA CACTACCTGA GGCTTTGACA 
TACTTGCTTG ATATCACTAC TCCACCCTCC CAACAACTGC TAAAAAAACT CTCCCAGCTG 
GTGACAGCAG AAGGAGATAA GCAAAGGCTG GAGGTGCTGT GTCAGAGCAC AGAAGAATAC 
AATAAATGGA AGTTTTACAA CAGCCCAAAC ATCCTGGAGG TGCTGGAGGA GTTCCCCTCT 
GCTGAGGTCT CTGCAGCTTT CCTGCTGACT CAGCTGCCAC TTCTGAAGCC CAGGTACTAT 
TCTGTCAGCT CCTCCTGTGA CCTGACAGCC AGAGAGATTC ATCTGACAGT TGCAGTTGTA 
AACTACAGGA CAAGAGATGG TGAAGGGCCT TTGCACCATG GAGTCTGCAG CACCTGGCTG 
AACACAATAG AGCTCAATGA AACCGTTCCC TGCTTCATCC GCAGTGCTAA TGAGTTCCAC 
CTCCCGGAGG AGCCATCCAA GCCCTGCATT CTGATCGGCT CAGGCACGGG GATTGCTCCC 
TTCAGGAGCT TCTGGCTGCA GCGCCTCTAT GACCTGGAGC ACAGAGGGCT CAGAGGAGGG 
AACATGACCC TGCTGTTCGG CTGCCGGCAT CCAGACCTGG ATCACATCTA CAGGGAAGAG 
ACTGAGGAGA TGAAGAGGAA GGGAGTCCTG AGTGATGTCT ACACAGCTTA CTCCAGACTG 
CCTGGCCAAG AGAAAGTGTA CGTCCAGGAC GTGCTGCAGG GGCAGCTGGC GGCCCGCGTG 
TGCGAGGTGC TGCAGGGGCA GCAGGGCCAT GTGTACGTGT GCGGGGACGT GCGCATGGCG 
CGGGACGTGG CCGGGGCGCT GCGGGCGCTG CTCGCCCGCG CCCTGCGCCT CAGCCAGCAG 
CAGGCCAGCG AGTTCCTGCA GCAGCTCAAG AGCCAAAAGC GATACCATGA AGATATATTT 
GGGGCTGTGT TCGCACATGA AGTCAAAAGA GCTTTGCAAC CCACCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_030287974.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030143834.1 Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X4, mRNA.

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CloneID	OTd32000
Clone ID Related Accession (Same CDS sequence)	XM_030287974.1
Accession Version	XM_030287974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	nitric oxide synthase, inducible isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCC CATGGCAGTT TGTGTTCAAA TCTCGTGCTG CCAAGACCCA GTACCCCCAG 
GAGAAGGATA TCAACAACAA CGTGGAGAAA AATAGGAAGA TCCATGGCTC AGAAAAGGAT 
GATGCCAAGT TGCACAATCC GAGCAAGAAG CAGATGGAGA GGCTGCCTAT CATAACTTCA 
GCAAAGCCCA GTGAGGGAGG AGAGAATCTC CCTTCAAATG ACATCAAACT TTCAAACCAA 
GTATCAGGAT GTCCAAGACA TGTAAGAGTA AGAAACTTGG AAAATGGATC CAGCTTTCTT 
GACACACTAC ACCTGACTGC AAAAGAGGTT ATCAATTGCC AGACCAAAGC CTGCCAAGGG 
TCACTCATGA CCCCAAAGGG CCTGGTGAGA GGTACCAGAG ATGGGCCGGT TCCACTACAG 
GATCTTTTAC CTCAGGCAAT AGACTTCCTC AAGCAATACT ACAGTTCATT TAAAGAGCCA 
AAAATCAAAG AAGAGCTGGG CCGGCTGGAA GCAGTGACCA AGGAGATAGA AACAACAGGA 
ACTTACCACC TGACAAAGGA TGAGTTGGCC TTTGCTGCCA AGCAGGCCTG GAGGAATGCC 
CCCAGGTGCA TTGGGAGGAT CCAGTGGTCC AACCTGCAGG TGTTTGATGC ACGAGACTGT 
AAAACAGCCA AGGAAATGTT TGAGCATATC TGTCATCATA TTCAGTATGC CACAAACAAT 
GGTAATATAA GGTCAGCCAT CACGGTGTTC CCGCACAGGA CGGACGGGCA GCACGATTTC 
CGTGTGTGGA ACAGCCAGCT GATCCGCTAC GCTGGCTACC AAATGTCAGA TGGCTCTGTC 
CTTGGAGACC CTGCTGCTGT GGAGTTCACT CAGTTGTGCA TTGAACTTGG GTGGAAGCCG 
AAATATGGCC GCTTTGATGT ACTTCCACTT GTTCTCCAAG CAAATGGCCA AGACCCAGAA 
ATATTTGAAT TACCTCCAGA AATTGTCCTC CAAGTGCCAA TGGAGCATCC AAAGTACAAG 
TGGTTTAAGG AGCTGGAGCT GAAGTGGTAC GCGCTGCCTG CTGTGGCCAA CATGCTTCTG 
GAGGCAGGAG GGCTGGAGTT CCCCGCATGC CCTTTCAATG GCTGGTACAT GGGGACAGAG 
ATCGGCGTGC GGGACTTCTG CGACGCGCAG CGCTACAACA TCCTGAAGGA GGTAGGGAGG 
AGAATGGGAC TGGAAACAAA TAAACTGTCA TCATTATGGA AGGACCGAGC TGTTGTAGAG 
ATCAATGTGG CTGTGCTTCA CAGCTTCCAG AGACAGAATG TAACCATCAT GGATCACCAC 
TCAGCCTCCG AGTCCTTCCT GAAGTACATG CAGAGCGAGT ACCGCGCGCG GGGCGGCTGC 
CCGGCAGATT GGGTGTGGAT CGTGCCTCCC ATGTCAGGCA GCATAACCCC CGTGTTCCAC 
CAAGAGATGT TGAACTATGT CCTCACTCCC TTCTATTACT ACCAGGTAGA TGCATGGAAA 
ACACATGTAT GGCATGATGA GACCCGAAGG CGAAAGAAAA AAGAAATAAA GTTTAGCATC 
TTGGCAAAGG CTGTGCTCTT TGCATCCTCA CTGATGCGAG GGGCAGTGGC CACCAGGGCC 
AAGGTCACCG TGATCTACGC GACAGAAACG GGCAAGTCGG AGACGCTCGC CAACAGCCTG 
TGCAGCCTGT TCAGCTGTGC CTTCAGCACC AAGATCCTCT GCATGGATGA GTACAATATT 
TGTGACCTGG AAAAAGAAAC ACTTCTTTTA GTGGTTACTA GCACTTTTGG AAATGGAGAT 
TCTCCAGGTA ACGGAAAGAC CTTGAAGGAC TCTTTGCTCA GACTAAAACT GCTGAGAAAT 
AAAATTAGGT ATGCTGTGTT TGGTCTGGGG TCCAGCATGT ACCCAGAGTT CTGTGCCTTT 
GCTCATGCCA TTGACCAAAA ACTGGCCCAA CTCGGGGCTT CCCAGCTCAC TCCAGTGGGT 
GAAGGGGATG AACTCAATGG GCAAGAAGAA GCTTTCCGCA GCTGGGCAGT CAGTGCTTTC 
AAGGCTGCCT GTGACATCTT TAACGTCCGT GGGAGACACA GTATTCAGTT GCCTAAGATC 
TACACCTCTG AGGAAAGGTG GGACCCTACC AATTACAGGC TAGTGCATGA CTCCCAGCCC 
ATGGACCTGG CTAAAGCTCT TGCAAACATT CATGCCAAGG ATGTAATTCC CATGAAGCTG 
AAATTTAGGC AGAATCTTCA AAGTTTGAAG TCCAGTCGTG TCACCATTCT GGTTAAACTT 
CAGTGTGAGA CAGATCAGGA AGTGCACTAC CTTCCCGGGG AGCACATCGG GATTTTCCCA 
GGCAACCAAG CAGAATTAGT CCATGGCATT ATTGCACATG TCAAGGATGC CCCTCCAGCT 
GATCAGACTA TCAGGCTTGA AACCTGCATT GATGGTGGGT ACTGGGCAAG TCACAAAAAG 
ATTCCAGCCT GCACACTACC TGAGGCTTTG ACATACTTGC TTGATATCAC TACTCCACCC 
TCCCAACAAC TGCTAAAAAA ACTCTCCCAG CTGGTGACAG CAGAAGGAGA TAAGCAAAGG 
CTGGAGGTGC TGTGTCAGAG CACAGAAGAA TACAATAAAT GGAAGTTTTA CAACAGCCCA 
AACATCCTGG AGGTGCTGGA GGAGTTCCCC TCTGCTGAGG TCTCTGCAGC TTTCCTGCTG 
ACTCAGCTGC CACTTCTGAA GCCCAGGTAC TATTCTGTCA GCTCCTCCTG TGACCTGACA 
GCCAGAGAGA TTCATCTGAC AGTTGCAGTT GTAAACTACA GGACAAGAGA TGGTGAAGGG 
CCTTTGCACC ATGGAGTCTG CAGCACCTGG CTGAACACAA TAGAGCTCAA TGAAACCGTT 
CCCTGCTTCA TCCGCAGTGC TAATGAGTTC CACCTCCCGG AGGAGCCATC CAAGCCCTGC 
ATTCTGATCG GCTCAGGCAC GGGGATTGCT CCCTTCAGGA GCTTCTGGCT GCAGCGCCTC 
TATGACCTGG AGCACAGAGG GCTCAGAGGA GGGAACATGA CCCTGCTGTT CGGCTGCCGG 
CATCCAGACC TGGATCACAT CTACAGGGAA GAGACTGAGG AGATGAAGAG GAAGGGAGTC 
CTGAGTGATG TCTACACAGC TTACTCCAGA CTGCCTGGCC AAGAGAAAGT GTACGTCCAG 
GACGTGCTGC AGGGGCAGCT GGCGGCCCGC GTGTGCGAGG TGCTGCAGGG GCAGCAGGGC 
CATGTGTACG TGTGCGGGGA CGTGCGCATG GCGCGGGACG TGGCCGGGGC GCTGCGGGCG 
CTGCTCGCCC GCGCCCTGCG CCTCAGCCAG CAGCAGGCCA GCGAGTTCCT GCAGCAGCTC 
AAGAGCCAAA AGCGATACCA TGAAGATATA TTTGGGGCTG TGTTCGCACA TGAAGTCAAA 
AGAGCTTTGC AACCCACCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030143834.1
CDS	1090..4533
Translation	MLCPWQFVFKSRAAKTQYPQEKDINNNVEKNRKIHGSEKDDAKLHNPSKKQMERLPIITSAKPSEGGENLPSNDIKLSNQVSGCPRHVRVRNLENGSSFLDTLHLTAKEVINCQTKACQGSLMTPKGLVRGTRDGPVPLQDLLPQAIDFLKQYYSSFKEPKIKEELGRLEAVTKEIETTGTYHLTKDELAFAAKQAWRNAPRCIGRIQWSNLQVFDARDCKTAKEMFEHICHHIQYATNNGNIRSAITVFPHRTDGQHDFRVWNSQLIRYAGYQMSDGSVLGDPAAVEFTQLCIELGWKPKYGRFDVLPLVLQANGQDPEIFELPPEIVLQVPMEHPKYKWFKELELKWYALPAVANMLLEAGGLEFPACPFNGWYMGTEIGVRDFCDAQRYNILKEVGRRMGLETNKLSSLWKDRAVVEINVAVLHSFQRQNVTIMDHHSASESFLKYMQSEYRARGGCPADWVWIVPPMSGSITPVFHQEMLNYVLTPFYYYQVDAWKTHVWHDETRRRKKKEIKFSILAKAVLFASSLMRGAVATRAKVTVIYATETGKSETLANSLCSLFSCAFSTKILCMDEYNICDLEKETLLLVVTSTFGNGDSPGNGKTLKDSLLRLKLLRNKIRYAVFGLGSSMYPEFCAFAHAIDQKLAQLGASQLTPVGEGDELNGQEEAFRSWAVSAFKAACDIFNVRGRHSIQLPKIYTSEERWDPTNYRLVHDSQPMDLAKALANIHAKDVIPMKLKFRQNLQSLKSSRVTILVKLQCETDQEVHYLPGEHIGIFPGNQAELVHGIIAHVKDAPPADQTIRLETCIDGGYWASHKKIPACTLPEALTYLLDITTPPSQQLLKKLSQLVTAEGDKQRLEVLCQSTEEYNKWKFYNSPNILEVLEEFPSAEVSAAFLLTQLPLLKPRYYSVSSSCDLTAREIHLTVAVVNYRTRDGEGPLHHGVCSTWLNTIELNETVPCFIRSANEFHLPEEPSKPCILIGSGTGIAPFRSFWLQRLYDLEHRGLRGGNMTLLFGCRHPDLDHIYREETEEMKRKGVLSDVYTAYSRLPGQEKVYVQDVLQGQLAARVCEVLQGQQGHVYVCGDVRMARDVAGALRALLARALRLSQQQASEFLQQLKSQKRYHEDIFGAVFAHEVKRALQPTS

Target ORF information:

RefSeq Version	XM_030287974.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata nitric oxide synthase 2 (NOS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030287974.1

ORF Insert Sequence:

ATGCTGTGCC CATGGCAGTT TGTGTTCAAA TCTCGTGCTG CCAAGACCCA GTACCCCCAG 
GAGAAGGATA TCAACAACAA CGTGGAGAAA AATAGGAAGA TCCATGGCTC AGAAAAGGAT 
GATGCCAAGT TGCACAATCC GAGCAAGAAG CAGATGGAGA GGCTGCCTAT CATAACTTCA 
GCAAAGCCCA GTGAGGGAGG AGAGAATCTC CCTTCAAATG ACATCAAACT TTCAAACCAA 
GTATCAGGAT GTCCAAGACA TGTAAGAGTA AGAAACTTGG AAAATGGATC CAGCTTTCTT 
GACACACTAC ACCTGACTGC AAAAGAGGTT ATCAATTGCC AGACCAAAGC CTGCCAAGGG 
TCACTCATGA CCCCAAAGGG CCTGGTGAGA GGTACCAGAG ATGGGCCGGT TCCACTACAG 
GATCTTTTAC CTCAGGCAAT AGACTTCCTC AAGCAATACT ACAGTTCATT TAAAGAGCCA 
AAAATCAAAG AAGAGCTGGG CCGGCTGGAA GCAGTGACCA AGGAGATAGA AACAACAGGA 
ACTTACCACC TGACAAAGGA TGAGTTGGCC TTTGCTGCCA AGCAGGCCTG GAGGAATGCC 
CCCAGGTGCA TTGGGAGGAT CCAGTGGTCC AACCTGCAGG TGTTTGATGC ACGAGACTGT 
AAAACAGCCA AGGAAATGTT TGAGCATATC TGTCATCATA TTCAGTATGC CACAAACAAT 
GGTAATATAA GGTCAGCCAT CACGGTGTTC CCGCACAGGA CGGACGGGCA GCACGATTTC 
CGTGTGTGGA ACAGCCAGCT GATCCGCTAC GCTGGCTACC AAATGTCAGA TGGCTCTGTC 
CTTGGAGACC CTGCTGCTGT GGAGTTCACT CAGTTGTGCA TTGAACTTGG GTGGAAGCCG 
AAATATGGCC GCTTTGATGT ACTTCCACTT GTTCTCCAAG CAAATGGCCA AGACCCAGAA 
ATATTTGAAT TACCTCCAGA AATTGTCCTC CAAGTGCCAA TGGAGCATCC AAAGTACAAG 
TGGTTTAAGG AGCTGGAGCT GAAGTGGTAC GCGCTGCCTG CTGTGGCCAA CATGCTTCTG 
GAGGCAGGAG GGCTGGAGTT CCCCGCATGC CCTTTCAATG GCTGGTACAT GGGGACAGAG 
ATCGGCGTGC GGGACTTCTG CGACGCGCAG CGCTACAACA TCCTGAAGGA GGTAGGGAGG 
AGAATGGGAC TGGAAACAAA TAAACTGTCA TCATTATGGA AGGACCGAGC TGTTGTAGAG 
ATCAATGTGG CTGTGCTTCA CAGCTTCCAG AGACAGAATG TAACCATCAT GGATCACCAC 
TCAGCCTCCG AGTCCTTCCT GAAGTACATG CAGAGCGAGT ACCGCGCGCG GGGCGGCTGC 
CCGGCAGATT GGGTGTGGAT CGTGCCTCCC ATGTCAGGCA GCATAACCCC CGTGTTCCAC 
CAAGAGATGT TGAACTATGT CCTCACTCCC TTCTATTACT ACCAGGTAGA TGCATGGAAA 
ACACATGTAT GGCATGATGA GACCCGAAGG CGAAAGAAAA AAGAAATAAA GTTTAGCATC 
TTGGCAAAGG CTGTGCTCTT TGCATCCTCA CTGATGCGAG GGGCAGTGGC CACCAGGGCC 
AAGGTCACCG TGATCTACGC GACAGAAACG GGCAAGTCGG AGACGCTCGC CAACAGCCTG 
TGCAGCCTGT TCAGCTGTGC CTTCAGCACC AAGATCCTCT GCATGGATGA GTACAATATT 
TGTGACCTGG AAAAAGAAAC ACTTCTTTTA GTGGTTACTA GCACTTTTGG AAATGGAGAT 
TCTCCAGGTA ACGGAAAGAC CTTGAAGGAC TCTTTGCTCA GACTAAAACT GCTGAGAAAT 
AAAATTAGGT ATGCTGTGTT TGGTCTGGGG TCCAGCATGT ACCCAGAGTT CTGTGCCTTT 
GCTCATGCCA TTGACCAAAA ACTGGCCCAA CTCGGGGCTT CCCAGCTCAC TCCAGTGGGT 
GAAGGGGATG AACTCAATGG GCAAGAAGAA GCTTTCCGCA GCTGGGCAGT CAGTGCTTTC 
AAGGCTGCCT GTGACATCTT TAACGTCCGT GGGAGACACA GTATTCAGTT GCCTAAGATC 
TACACCTCTG AGGAAAGGTG GGACCCTACC AATTACAGGC TAGTGCATGA CTCCCAGCCC 
ATGGACCTGG CTAAAGCTCT TGCAAACATT CATGCCAAGG ATGTAATTCC CATGAAGCTG 
AAATTTAGGC AGAATCTTCA AAGTTTGAAG TCCAGTCGTG TCACCATTCT GGTTAAACTT 
CAGTGTGAGA CAGATCAGGA AGTGCACTAC CTTCCCGGGG AGCACATCGG GATTTTCCCA 
GGCAACCAAG CAGAATTAGT CCATGGCATT ATTGCACATG TCAAGGATGC CCCTCCAGCT 
GATCAGACTA TCAGGCTTGA AACCTGCATT GATGGTGGGT ACTGGGCAAG TCACAAAAAG 
ATTCCAGCCT GCACACTACC TGAGGCTTTG ACATACTTGC TTGATATCAC TACTCCACCC 
TCCCAACAAC TGCTAAAAAA ACTCTCCCAG CTGGTGACAG CAGAAGGAGA TAAGCAAAGG 
CTGGAGGTGC TGTGTCAGAG CACAGAAGAA TACAATAAAT GGAAGTTTTA CAACAGCCCA 
AACATCCTGG AGGTGCTGGA GGAGTTCCCC TCTGCTGAGG TCTCTGCAGC TTTCCTGCTG 
ACTCAGCTGC CACTTCTGAA GCCCAGGTAC TATTCTGTCA GCTCCTCCTG TGACCTGACA 
GCCAGAGAGA TTCATCTGAC AGTTGCAGTT GTAAACTACA GGACAAGAGA TGGTGAAGGG 
CCTTTGCACC ATGGAGTCTG CAGCACCTGG CTGAACACAA TAGAGCTCAA TGAAACCGTT 
CCCTGCTTCA TCCGCAGTGC TAATGAGTTC CACCTCCCGG AGGAGCCATC CAAGCCCTGC 
ATTCTGATCG GCTCAGGCAC GGGGATTGCT CCCTTCAGGA GCTTCTGGCT GCAGCGCCTC 
TATGACCTGG AGCACAGAGG GCTCAGAGGA GGGAACATGA CCCTGCTGTT CGGCTGCCGG 
CATCCAGACC TGGATCACAT CTACAGGGAA GAGACTGAGG AGATGAAGAG GAAGGGAGTC 
CTGAGTGATG TCTACACAGC TTACTCCAGA CTGCCTGGCC AAGAGAAAGT GTACGTCCAG 
GACGTGCTGC AGGGGCAGCT GGCGGCCCGC GTGTGCGAGG TGCTGCAGGG GCAGCAGGGC 
CATGTGTACG TGTGCGGGGA CGTGCGCATG GCGCGGGACG TGGCCGGGGC GCTGCGGGCG 
CTGCTCGCCC GCGCCCTGCG CCTCAGCCAG CAGCAGGCCA GCGAGTTCCT GCAGCAGCTC 
AAGAGCCAAA AGCGATACCA TGAAGATATA TTTGGGGCTG TGTTCGCACA TGAAGTCAAA 
AGAGCTTTGC AACCCACCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NOS2 cDNA ORF clone, Taeniopygia guttata(zebra finch)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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