TLR4 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR4
Entrez Gene ID	399541
Full Name	toll like receptor 4
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113039.2	NP_001106510.2	toll-like receptor 4 isoform 1 precursor
NM_001293316.1	NP_001280245.1	toll-like receptor 4 isoform 2
NM_001293317.1	NP_001280246.1	toll-like receptor 4 isoform 3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Flaxseed Oil Attenuates Intestinal Damage and Inflammation by Regulating Necroptosis and TLR4/NOD Signaling Pathways Following Lipopolysaccharide Challenge in a Piglet Model.
Zhu H, Wang H, Wang S, Tu Z, Zhang L, Wang X, Hou Y, Wang C, Chen J, Liu Y
Molecular nutrition & food research62(9)e1700814(2018 05)

Activation of Porcine Alveolar Macrophages by Actinobacillus pleuropneumoniae Lipopolysaccharide via the Toll-Like Receptor 4/NF-κB-Mediated Pathway.
Li B, Fang J, Zuo Z, Yin S, He T, Yang M, Deng J, Shen L, Ma X, Yu S, Wang Y, Ren Z
Infection and immunity86(3)(2018 03)

Lipopolysaccharide Upregulated Intestinal Epithelial Cell Expression of Fn14 and Activation of Fn14 Signaling Amplify Intestinal TLR4-Mediated Inflammation.
Qi X, Qin L, Du R, Chen Y, Lei M, Deng M, Wang J
Frontiers in cellular and infection microbiology7315(2017)

Lactobacillus acidophilus modulates inflammatory activity by regulating the TLR4 and NF-κB expression in porcine peripheral blood mononuclear cells after lipopolysaccharide challenge.
Lee SI, Kim HS, Koo JM, Kim IH
The British journal of nutrition115(4)567-75(2016 Feb)

Differential expression of Toll-like receptor 4 signaling pathway genes in Escherichia coli F18-resistant and - sensitive Meishan piglets.
Liu Y, Gan LN, Qin WY, Sun SY, Zhu GQ, Wu SL, Bao WB
Polish journal of veterinary sciences19(2)303-8(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TLR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe00827	NM_001293317.1 Latest version!	Sus scrofa toll like receptor 4 (TLR4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OSe00826	NM_001113039.2 Latest version!	Sus scrofa toll like receptor 4 (TLR4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSe00828	NM_001293316.1 Latest version!	Sus scrofa toll like receptor 4 (TLR4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR4 ( NM_001293317.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001280246.1 Sus scrofa toll like receptor 4 (TLR4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OSe00827
Clone ID Related Accession (Same CDS sequence)	NM_001293317.1
Accession Version	NM_001293317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Sus scrofa(pig)
Product	toll-like receptor 4 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK396573.1 and DB797683.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 3, which lacks a large internal in-frame segment and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK389696.1, BW958011.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2041381, SAMEA3179989 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTCCTC GCATCCGCCT GGCTGTGGCC ACGATCCCAG CCATGGCCTT TCTCTCCTGC 
CTGAGATCTG AGAGCTGGGA CCCTTGCGTG CAGGTGGTTC CTAACATTAG TTACCAATGC 
ATGGAGCTGA ATTTCTACAA AATCCCTGAC AACATCCCCA CATCAGTCAA GATACTGGAC 
CTGAGCTTTA ACTACCTGAG TCATTTAGAC AGCAATAGCT TCTCCAGCTT TCCAGAACTG 
CAGGTGCTGG ATTTATCCAG CCAGGACGAA GACTGGGTGA GGAATGAACT GGTAAAGAAC 
TTGGAGGAGG GGGTGCCCCC TTTTCATCTC TGCCTTCACT ACAGAGACTT CATTCCCGGT 
GTGGCCATCG CTGCTAACAT CATCCAGGAA GGTTTCCACA AAAGTCGGAA GGTTATTGTC 
GTGGTGTCCC AGCACTTCAT ACAGAGCCGA TGGTGTATCT TTGAGTATGA GATTGCCCAG 
ACCTGGCAGT TTCTGAGGAG TCATGCCGGC ATCATCTTCA TCGTCCTGCA GAAGTTGGAG 
AAGTCCCTGC TGCGGCAGCA GGTGGAGCTG TATCGCCTTC TCAGCAGGAA TACCTACCTG 
GAGTGGGAGG ACAGCGTCCT GGGGCGGCAC ATCTTCTGGA GACGACTCAA GAAAGCCTTG 
CTGGATGGTA AACCATGGAG TCCAGAAGGA ACAGAGGATT CAGAAAGCAA CCAGCATGAT 
ACAACAGCCT TCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280246.1
CDS	249..986
Translation	MIPRIRLAVATIPAMAFLSCLRSESWDPCVQVVPNISYQCMELNFYKIPDNIPTSVKILDLSFNYLSHLDSNSFSSFPELQVLDLSSQDEDWVRNELVKNLEEGVPPFHLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLRSHAGIIFIVLQKLEKSLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLKKALLDGKPWSPEGTEDSESNQHDTTAFT

Target ORF information:

RefSeq Version	NM_001293317.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa toll like receptor 4 (TLR4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293317.1

ORF Insert Sequence:

ATGATTCCTC GCATCCGCCT GGCTGTGGCC ACGATCCCAG CCATGGCCTT TCTCTCCTGC 
CTGAGATCTG AGAGCTGGGA CCCTTGCGTG CAGGTGGTTC CTAACATTAG TTACCAATGC 
ATGGAGCTGA ATTTCTACAA AATCCCTGAC AACATCCCCA CATCAGTCAA GATACTGGAC 
CTGAGCTTTA ACTACCTGAG TCATTTAGAC AGCAATAGCT TCTCCAGCTT TCCAGAACTG 
CAGGTGCTGG ATTTATCCAG CCAGGACGAA GACTGGGTGA GGAATGAACT GGTAAAGAAC 
TTGGAGGAGG GGGTGCCCCC TTTTCATCTC TGCCTTCACT ACAGAGACTT CATTCCCGGT 
GTGGCCATCG CTGCTAACAT CATCCAGGAA GGTTTCCACA AAAGTCGGAA GGTTATTGTC 
GTGGTGTCCC AGCACTTCAT ACAGAGCCGA TGGTGTATCT TTGAGTATGA GATTGCCCAG 
ACCTGGCAGT TTCTGAGGAG TCATGCCGGC ATCATCTTCA TCGTCCTGCA GAAGTTGGAG 
AAGTCCCTGC TGCGGCAGCA GGTGGAGCTG TATCGCCTTC TCAGCAGGAA TACCTACCTG 
GAGTGGGAGG ACAGCGTCCT GGGGCGGCAC ATCTTCTGGA GACGACTCAA GAAAGCCTTG 
CTGGATGGTA AACCATGGAG TCCAGAAGGA ACAGAGGATT CAGAAAGCAA CCAGCATGAT 
ACAACAGCCT TCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( NM_001113039.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001106510.2 Sus scrofa toll like receptor 4 (TLR4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OSe00826
Clone ID Related Accession (Same CDS sequence)	NM_001113039.2
Accession Version	NM_001113039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Sus scrofa(pig)
Product	toll-like receptor 4 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK396573.1. On Jun 7, 2014 this sequence version replaced NM_001113039.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK396573.1, AB188301.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876826 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTCCTC GCATCCGCCT GGCTGTGGCC ACGATCCCAG CCATGGCCTT TCTCTCCTGC 
CTGAGATCTG AGAGCTGGGA CCCTTGCGTG CAGGTGGTTC CTAACATTAG TTACCAATGC 
ATGGAGCTGA ATTTCTACAA AATCCCTGAC AACATCCCCA CATCAGTCAA GATACTGGAC 
CTGAGCTTTA ACTACCTGAG TCATTTAGAC AGCAATAGCT TCTCCAGCTT TCCAGAACTG 
CAGGTGCTGG ATTTATCCAG ATGTGAAATT CAGACAATTG ACGATGATGC ATATCAGGGC 
CTAAATTACC TTTCCACCTT GATACTGACG GGAAATCCTA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTACCAAG TTTACAGAAG CTGGTTGCCG TTGAGACAAA CCTAGCGTCT 
CTAGAGGACT TCCCCATTGG ACATCTTAAA ACCTTGAAGG AGCTTAATGT GGCTCACAAT 
CATATCCATT CTTTCAAGTT ACCTGAATAT TTTTCTAACC TGCCCAACCT GGAGCATTTG 
GATCTTTCTA AGAACAAAAT CGAAAATATT TATCATGAAC ACTTGCAGGT TCTACATCAA 
GTGCCCCTAC TCAATCTCTC TTTAGACTTG TCCCTGAACC CTTTAAACTT TATTGAACCA 
GGTGCCTTTA ACAAAATTAG GCTCAATGGA TTGACTTTGC GAAGTAATTT TAATAGTTCG 
GATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTCAA AAATCAATCA GTTGGTTTTG 
GGAGAATTTA AAAATGAAAG GAACTTGGAA AGTTTTGACA AATCTGTCCT GGAGGAACTG 
TGCAATTTGA CCCTTGAACA GTTCCGGATA GCACACTTTG GTGAATTCCC AGATGATGTT 
TCTGACTTAT TTAATTGTTT GGCAAATGCC TCTGTGATTT CTCTGTTGAG TCTGAATTTG 
CGTGGTCTAG AAGCCCTTCC TAATGATTTC AGGTGGCAAC ACTTAGAAGT GGTTAACTGT 
AAACTTCAAC AATTTCCTGC ATTGAAGTTC AACTCTCTCA AAAAGTTTGT TTTCAAAGAC 
AATAAACATA TGCACACTTT TACTGAAATT AACCTGCCAA ACCTTCAGTT TCTAGATCTC 
AGTGGAAATC ACTTGAGCTT TAAAGGTTGC TGTTCTCACA ATGAATTTGG GACAACCAAA 
CTGAAGCATT TAGATCTGAG CTTCAATGAA ATCATTACCA TGAAGTCAAA CTTCATGGGC 
TTAGAGCAAC TAGAATACCT GGATTTCCAG CATTCCAGTT TGAAACAGGC TAATGACTTT 
TCCATATTCC TATCACTCAG AAACCTCCAT TACCTTGATA TTTCTTATAC TAACATCCAC 
GTTGTCTTCC GTGGCATTTT TGCTGGCTTG GTCAGCCTCC AAACCTTGAA AATGGCTGGC 
AATTCTTTTC AGAACAACTT GCTTCCTGAT GTCTTCACAG ATCTGACTAA CTTAATCCTC 
CTGGACCTCT CTAAGTGTCA ACTGGAACAG GTATCCCAGA GGGCATTTCA TTCCCTCCCT 
AGGCTTCAGG TGCTAAATAT GAGTCACAAC AGACTCTTGT TCTTGGATAC ACTTCCTTAT 
AAACCACTCC ACTCCCTCAG GATTCTGGAT TGCAGTTACA ATCTTATCGT GGCCTCCAAG 
GAACAAGAAC TACAGCATCT GCCAAGGAGC CTAGCTTTTT TAAATCTCAC TAAGAATGAC 
TTTTCTTGTG CGTGTGAACA CCAGACTTTC TTGCAGTGGG TCAAGGACCA GAAGCAGCTC 
CTTGTGGGAG CTGAGCAAAT GGTGTGTACA CAACCTTTAG AAATGCAGGA CCTGCCTGTG 
CTGAGTTTCA GGAACGCCAC CTGTCAGATA AGCGAGGCCG TCATTAGTGC GTCAGTTCTC 
ACCTTCCTCC TGGTATCTGT GGCAGGAATC CTGGTCTATA AGTTCTATTT CCACTTGTTG 
CTTTTTGTGG GCTGCAAAAA ATATGGCAGA GGTGAAAGCA CCTATGACGC CTTTGTTATC 
TACTCCAGCC AGGACGAAGA CTGGGTGAGG AATGAACTGG TAAAGAACTT GGAGGAGGGG 
GTGCCCCCTT TTCATCTCTG CCTTCACTAC AGAGACTTCA TTCCCGGTGT GGCCATCGCT 
GCTAACATCA TCCAGGAAGG TTTCCACAAA AGTCGGAAGG TTATTGTCGT GGTGTCCCAG 
CACTTCATAC AGAGCCGATG GTGTATCTTT GAGTATGAGA TTGCCCAGAC CTGGCAGTTT 
CTGAGGAGTC ATGCCGGCAT CATCTTCATC GTCCTGCAGA AGTTGGAGAA GTCCCTGCTG 
CGGCAGCAGG TGGAGCTGTA TCGCCTTCTC AGCAGGAATA CCTACCTGGA GTGGGAGGAC 
AGCGTCCTGG GGCGGCACAT CTTCTGGAGA CGACTCAAGA AAGCCTTGCT GGATGGTAAA 
CCATGGAGTC CAGAAGGAAC AGAGGATTCA GAAAGCAACC AGCATGATAC AACAGCCTTC 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106510.2
CDS	249..2774
Translation	MIPRIRLAVATIPAMAFLSCLRSESWDPCVQVVPNISYQCMELNFYKIPDNIPTSVKILDLSFNYLSHLDSNSFSSFPELQVLDLSRCEIQTIDDDAYQGLNYLSTLILTGNPIQSLALGAFSGLPSLQKLVAVETNLASLEDFPIGHLKTLKELNVAHNHIHSFKLPEYFSNLPNLEHLDLSKNKIENIYHEHLQVLHQVPLLNLSLDLSLNPLNFIEPGAFNKIRLNGLTLRSNFNSSDVMKTCIQGLAGSKINQLVLGEFKNERNLESFDKSVLEELCNLTLEQFRIAHFGEFPDDVSDLFNCLANASVISLLSLNLRGLEALPNDFRWQHLEVVNCKLQQFPALKFNSLKKFVFKDNKHMHTFTEINLPNLQFLDLSGNHLSFKGCCSHNEFGTTKLKHLDLSFNEIITMKSNFMGLEQLEYLDFQHSSLKQANDFSIFLSLRNLHYLDISYTNIHVVFRGIFAGLVSLQTLKMAGNSFQNNLLPDVFTDLTNLILLDLSKCQLEQVSQRAFHSLPRLQVLNMSHNRLLFLDTLPYKPLHSLRILDCSYNLIVASKEQELQHLPRSLAFLNLTKNDFSCACEHQTFLQWVKDQKQLLVGAEQMVCTQPLEMQDLPVLSFRNATCQISEAVISASVLTFLLVSVAGILVYKFYFHLLLFVGCKKYGRGESTYDAFVIYSSQDEDWVRNELVKNLEEGVPPFHLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLRSHAGIIFIVLQKLEKSLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLKKALLDGKPWSPEGTEDSESNQHDTTAFT

Target ORF information:

RefSeq Version	NM_001113039.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa toll like receptor 4 (TLR4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113039.2

ORF Insert Sequence:

ATGATTCCTC GCATCCGCCT GGCTGTGGCC ACGATCCCAG CCATGGCCTT TCTCTCCTGC 
CTGAGATCTG AGAGCTGGGA CCCTTGCGTG CAGGTGGTTC CTAACATTAG TTACCAATGC 
ATGGAGCTGA ATTTCTACAA AATCCCTGAC AACATCCCCA CATCAGTCAA GATACTGGAC 
CTGAGCTTTA ACTACCTGAG TCATTTAGAC AGCAATAGCT TCTCCAGCTT TCCAGAACTG 
CAGGTGCTGG ATTTATCCAG ATGTGAAATT CAGACAATTG ACGATGATGC ATATCAGGGC 
CTAAATTACC TTTCCACCTT GATACTGACG GGAAATCCTA TCCAGAGTTT AGCCCTGGGA 
GCCTTTTCTG GACTACCAAG TTTACAGAAG CTGGTTGCCG TTGAGACAAA CCTAGCGTCT 
CTAGAGGACT TCCCCATTGG ACATCTTAAA ACCTTGAAGG AGCTTAATGT GGCTCACAAT 
CATATCCATT CTTTCAAGTT ACCTGAATAT TTTTCTAACC TGCCCAACCT GGAGCATTTG 
GATCTTTCTA AGAACAAAAT CGAAAATATT TATCATGAAC ACTTGCAGGT TCTACATCAA 
GTGCCCCTAC TCAATCTCTC TTTAGACTTG TCCCTGAACC CTTTAAACTT TATTGAACCA 
GGTGCCTTTA ACAAAATTAG GCTCAATGGA TTGACTTTGC GAAGTAATTT TAATAGTTCG 
GATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTCAA AAATCAATCA GTTGGTTTTG 
GGAGAATTTA AAAATGAAAG GAACTTGGAA AGTTTTGACA AATCTGTCCT GGAGGAACTG 
TGCAATTTGA CCCTTGAACA GTTCCGGATA GCACACTTTG GTGAATTCCC AGATGATGTT 
TCTGACTTAT TTAATTGTTT GGCAAATGCC TCTGTGATTT CTCTGTTGAG TCTGAATTTG 
CGTGGTCTAG AAGCCCTTCC TAATGATTTC AGGTGGCAAC ACTTAGAAGT GGTTAACTGT 
AAACTTCAAC AATTTCCTGC ATTGAAGTTC AACTCTCTCA AAAAGTTTGT TTTCAAAGAC 
AATAAACATA TGCACACTTT TACTGAAATT AACCTGCCAA ACCTTCAGTT TCTAGATCTC 
AGTGGAAATC ACTTGAGCTT TAAAGGTTGC TGTTCTCACA ATGAATTTGG GACAACCAAA 
CTGAAGCATT TAGATCTGAG CTTCAATGAA ATCATTACCA TGAAGTCAAA CTTCATGGGC 
TTAGAGCAAC TAGAATACCT GGATTTCCAG CATTCCAGTT TGAAACAGGC TAATGACTTT 
TCCATATTCC TATCACTCAG AAACCTCCAT TACCTTGATA TTTCTTATAC TAACATCCAC 
GTTGTCTTCC GTGGCATTTT TGCTGGCTTG GTCAGCCTCC AAACCTTGAA AATGGCTGGC 
AATTCTTTTC AGAACAACTT GCTTCCTGAT GTCTTCACAG ATCTGACTAA CTTAATCCTC 
CTGGACCTCT CTAAGTGTCA ACTGGAACAG GTATCCCAGA GGGCATTTCA TTCCCTCCCT 
AGGCTTCAGG TGCTAAATAT GAGTCACAAC AGACTCTTGT TCTTGGATAC ACTTCCTTAT 
AAACCACTCC ACTCCCTCAG GATTCTGGAT TGCAGTTACA ATCTTATCGT GGCCTCCAAG 
GAACAAGAAC TACAGCATCT GCCAAGGAGC CTAGCTTTTT TAAATCTCAC TAAGAATGAC 
TTTTCTTGTG CGTGTGAACA CCAGACTTTC TTGCAGTGGG TCAAGGACCA GAAGCAGCTC 
CTTGTGGGAG CTGAGCAAAT GGTGTGTACA CAACCTTTAG AAATGCAGGA CCTGCCTGTG 
CTGAGTTTCA GGAACGCCAC CTGTCAGATA AGCGAGGCCG TCATTAGTGC GTCAGTTCTC 
ACCTTCCTCC TGGTATCTGT GGCAGGAATC CTGGTCTATA AGTTCTATTT CCACTTGTTG 
CTTTTTGTGG GCTGCAAAAA ATATGGCAGA GGTGAAAGCA CCTATGACGC CTTTGTTATC 
TACTCCAGCC AGGACGAAGA CTGGGTGAGG AATGAACTGG TAAAGAACTT GGAGGAGGGG 
GTGCCCCCTT TTCATCTCTG CCTTCACTAC AGAGACTTCA TTCCCGGTGT GGCCATCGCT 
GCTAACATCA TCCAGGAAGG TTTCCACAAA AGTCGGAAGG TTATTGTCGT GGTGTCCCAG 
CACTTCATAC AGAGCCGATG GTGTATCTTT GAGTATGAGA TTGCCCAGAC CTGGCAGTTT 
CTGAGGAGTC ATGCCGGCAT CATCTTCATC GTCCTGCAGA AGTTGGAGAA GTCCCTGCTG 
CGGCAGCAGG TGGAGCTGTA TCGCCTTCTC AGCAGGAATA CCTACCTGGA GTGGGAGGAC 
AGCGTCCTGG GGCGGCACAT CTTCTGGAGA CGACTCAAGA AAGCCTTGCT GGATGGTAAA 
CCATGGAGTC CAGAAGGAAC AGAGGATTCA GAAAGCAACC AGCATGATAC AACAGCCTTC 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR4 ( NM_001293316.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001280245.1 Sus scrofa toll like receptor 4 (TLR4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OSe00828
Clone ID Related Accession (Same CDS sequence)	NM_001293316.1
Accession Version	NM_001293316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Sus scrofa(pig)
Product	toll-like receptor 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK396573.1 and HM026368.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK343907.1, HM026368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2040857, SAMEA2040998 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAACTT GTATTCAAGG TCTGGCTGGT TCAAAAATCA ATCAGTTGGT TTTGGGAGAA 
TTTAAAAATG AAAGGAACTT GGAAAGTTTT GACAAATCTG TCCTGGAGGA ACTGTGCAAT 
TTGACCCTTG AACAGTTCCG GATAGCACAC TTTGGTGAAT TCCCAGATGA TGTTTCTGAC 
TTATTTAATT GTTTGGCAAA TGCCTCTGTG ATTTCTCTGT TGAGTCTGAA TTTGCGTGGT 
CTAGAAGCCC TTCCTAATGA TTTCAGGTGG CAACACTTAG AAGTGGTTAA CTGTAAACTT 
CAACAATTTC CTGCATTGAA GTTCAACTCT CTCAAAAAGT TTGTTTTCAA AGACAATAAA 
CATATGCACA CTTTTACTGA AATTAACCTG CCAAACCTTC AGTTTCTAGA TCTCAGTGGA 
AATCACTTGA GCTTTAAAGG TTGCTGTTCT CACAATGAAT TTGGGACAAC CAAACTGAAG 
CATTTAGATC TGAGCTTCAA TGAAATCATT ACCATGAAGT CAAACTTCAT GGGCTTAGAG 
CAACTAGAAT ACCTGGATTT CCAGCATTCC AGTTTGAAAC AGGCTAATGA CTTTTCCATA 
TTCCTATCAC TCAGAAACCT CCATTACCTT GATATTTCTT ATACTAACAT CCACGTTGTC 
TTCCGTGGCA TTTTTGCTGG CTTGGTCAGC CTCCAAACCT TGAAAATGGC TGGCAATTCT 
TTTCAGAACA ACTTGCTTCC TGATGTCTTC ACAGATCTGA CTAACTTAAT CCTCCTGGAC 
CTCTCTAAGT GTCAACTGGA ACAGGTATCC CAGAGGGCAT TTCATTCCCT CCCTAGGCTT 
CAGGTGCTAA ATATGAGTCA CAACAGACTC TTGTTCTTGG ATACACTTCC TTATAAACCA 
CTCCACTCCC TCAGGATTCT GGATTGCAGT TACAATCTTA TCGTGGCCTC CAAGGAACAA 
GAACTACAGC ATCTGCCAAG GAGCCTAGCT TTTTTAAATC TCACTAAGAA TGACTTTTCT 
TGTGCGTGTG AACACCAGAC TTTCTTGCAG TGGGTCAAGG ACCAGAAGCA GCTCCTTGTG 
GGAGCTGAGC AAATGGTGTG TACACAACCT TTAGAAATGC AGGACCTGCC TGTGCTGAGT 
TTCAGGAACG CCACCTGTCA GATAAGCGAG GCCGTCATTA GTGCGTCAGT TCTCACCTTC 
CTCCTGGTAT CTGTGGCAGG AATCCTGGTC TATAAGTTCT ATTTCCACTT GTTGCTTTTT 
GTGGGCTGCA AAAAATATGG CAGAGGTGAA AGCACCTATG ACGCCTTTGT TATCTACTCC 
AGCCAGGACG AAGACTGGGT GAGGAATGAA CTGGTAAAGA ACTTGGAGGA GGGGGTGCCC 
CCTTTTCATC TCTGCCTTCA CTACAGAGAC TTCATTCCCG GTGTGGCCAT CGCTGCTAAC 
ATCATCCAGG AAGGTTTCCA CAAAAGTCGG AAGGTTATTG TCGTGGTGTC CCAGCACTTC 
ATACAGAGCC GATGGTGTAT CTTTGAGTAT GAGATTGCCC AGACCTGGCA GTTTCTGAGG 
AGTCATGCCG GCATCATCTT CATCGTCCTG CAGAAGTTGG AGAAGTCCCT GCTGCGGCAG 
CAGGTGGAGC TGTATCGCCT TCTCAGCAGG AATACCTACC TGGAGTGGGA GGACAGCGTC 
CTGGGGCGGC ACATCTTCTG GAGACGACTC AAGAAAGCCT TGCTGGATGG TAAACCATGG 
AGTCCAGAAG GAACAGAGGA TTCAGAAAGC AACCAGCATG ATACAACAGC CTTCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280245.1
CDS	808..2607
Translation	MKTCIQGLAGSKINQLVLGEFKNERNLESFDKSVLEELCNLTLEQFRIAHFGEFPDDVSDLFNCLANASVISLLSLNLRGLEALPNDFRWQHLEVVNCKLQQFPALKFNSLKKFVFKDNKHMHTFTEINLPNLQFLDLSGNHLSFKGCCSHNEFGTTKLKHLDLSFNEIITMKSNFMGLEQLEYLDFQHSSLKQANDFSIFLSLRNLHYLDISYTNIHVVFRGIFAGLVSLQTLKMAGNSFQNNLLPDVFTDLTNLILLDLSKCQLEQVSQRAFHSLPRLQVLNMSHNRLLFLDTLPYKPLHSLRILDCSYNLIVASKEQELQHLPRSLAFLNLTKNDFSCACEHQTFLQWVKDQKQLLVGAEQMVCTQPLEMQDLPVLSFRNATCQISEAVISASVLTFLLVSVAGILVYKFYFHLLLFVGCKKYGRGESTYDAFVIYSSQDEDWVRNELVKNLEEGVPPFHLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLRSHAGIIFIVLQKLEKSLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLKKALLDGKPWSPEGTEDSESNQHDTTAFT

Target ORF information:

RefSeq Version	NM_001293316.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa toll like receptor 4 (TLR4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293316.1

ORF Insert Sequence:

ATGAAAACTT GTATTCAAGG TCTGGCTGGT TCAAAAATCA ATCAGTTGGT TTTGGGAGAA 
TTTAAAAATG AAAGGAACTT GGAAAGTTTT GACAAATCTG TCCTGGAGGA ACTGTGCAAT 
TTGACCCTTG AACAGTTCCG GATAGCACAC TTTGGTGAAT TCCCAGATGA TGTTTCTGAC 
TTATTTAATT GTTTGGCAAA TGCCTCTGTG ATTTCTCTGT TGAGTCTGAA TTTGCGTGGT 
CTAGAAGCCC TTCCTAATGA TTTCAGGTGG CAACACTTAG AAGTGGTTAA CTGTAAACTT 
CAACAATTTC CTGCATTGAA GTTCAACTCT CTCAAAAAGT TTGTTTTCAA AGACAATAAA 
CATATGCACA CTTTTACTGA AATTAACCTG CCAAACCTTC AGTTTCTAGA TCTCAGTGGA 
AATCACTTGA GCTTTAAAGG TTGCTGTTCT CACAATGAAT TTGGGACAAC CAAACTGAAG 
CATTTAGATC TGAGCTTCAA TGAAATCATT ACCATGAAGT CAAACTTCAT GGGCTTAGAG 
CAACTAGAAT ACCTGGATTT CCAGCATTCC AGTTTGAAAC AGGCTAATGA CTTTTCCATA 
TTCCTATCAC TCAGAAACCT CCATTACCTT GATATTTCTT ATACTAACAT CCACGTTGTC 
TTCCGTGGCA TTTTTGCTGG CTTGGTCAGC CTCCAAACCT TGAAAATGGC TGGCAATTCT 
TTTCAGAACA ACTTGCTTCC TGATGTCTTC ACAGATCTGA CTAACTTAAT CCTCCTGGAC 
CTCTCTAAGT GTCAACTGGA ACAGGTATCC CAGAGGGCAT TTCATTCCCT CCCTAGGCTT 
CAGGTGCTAA ATATGAGTCA CAACAGACTC TTGTTCTTGG ATACACTTCC TTATAAACCA 
CTCCACTCCC TCAGGATTCT GGATTGCAGT TACAATCTTA TCGTGGCCTC CAAGGAACAA 
GAACTACAGC ATCTGCCAAG GAGCCTAGCT TTTTTAAATC TCACTAAGAA TGACTTTTCT 
TGTGCGTGTG AACACCAGAC TTTCTTGCAG TGGGTCAAGG ACCAGAAGCA GCTCCTTGTG 
GGAGCTGAGC AAATGGTGTG TACACAACCT TTAGAAATGC AGGACCTGCC TGTGCTGAGT 
TTCAGGAACG CCACCTGTCA GATAAGCGAG GCCGTCATTA GTGCGTCAGT TCTCACCTTC 
CTCCTGGTAT CTGTGGCAGG AATCCTGGTC TATAAGTTCT ATTTCCACTT GTTGCTTTTT 
GTGGGCTGCA AAAAATATGG CAGAGGTGAA AGCACCTATG ACGCCTTTGT TATCTACTCC 
AGCCAGGACG AAGACTGGGT GAGGAATGAA CTGGTAAAGA ACTTGGAGGA GGGGGTGCCC 
CCTTTTCATC TCTGCCTTCA CTACAGAGAC TTCATTCCCG GTGTGGCCAT CGCTGCTAAC 
ATCATCCAGG AAGGTTTCCA CAAAAGTCGG AAGGTTATTG TCGTGGTGTC CCAGCACTTC 
ATACAGAGCC GATGGTGTAT CTTTGAGTAT GAGATTGCCC AGACCTGGCA GTTTCTGAGG 
AGTCATGCCG GCATCATCTT CATCGTCCTG CAGAAGTTGG AGAAGTCCCT GCTGCGGCAG 
CAGGTGGAGC TGTATCGCCT TCTCAGCAGG AATACCTACC TGGAGTGGGA GGACAGCGTC 
CTGGGGCGGC ACATCTTCTG GAGACGACTC AAGAAAGCCT TGCTGGATGG TAAACCATGG 
AGTCCAGAAG GAACAGAGGA TTCAGAAAGC AACCAGCATG ATACAACAGC CTTCACTTAA