NEFL cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFL
Entrez Gene ID	100521224
Full Name	neurofilament light
Synonyms	NF-L
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 14 Map Location: 14\|14

mRNA and Protein(s)

mRNA	Protein	Name
NM_001244331.1	NP_001231260.1	neurofilament light polypeptide

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags.
Gorodkin J, Cirera S, Hedegaard J, Gilchrist MJ, Panitz F, Jørgensen C, Scheibye-Knudsen K, Arvin T, Lumholdt S, Sawera M, Green T, Nielsen BJ, Havgaard JH, Rosenkilde C, Wang J, Li H, Li R, Liu B, Hu S, Dong W, Li W, Yu J, Wang J, Staefeldt HH, Wernersson R, Madsen LB, Thomsen B, Hornshøj H, Bujie Z, Wang X, Wang X, Bolund L, Brunak S, Yang H, Bendixen C, Fredholm M
Genome biology8(4)R45(2007)

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

Specific recognition of coiled coils by infrared spectroscopy: analysis of the three structural domains of type III intermediate filament proteins.
Heimburg T, Schuenemann J, Weber K, Geisler N
Biochemistry35(5)1375-82(1996 Feb)

The complete amino acid sequence of the major mammalian neurofilament protein (NF-L).
Geisler N, Plessmann U, Weber K
FEBS letters182(2)475-8(1985 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NEFL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe29053	NM_001244331.1 Latest version!	Sus scrofa neurofilament light (NEFL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFL ( NM_001244331.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001231260.1 Sus scrofa neurofilament light (NEFL), mRNA.

Price & Availability↑

CloneID	OSe29053
Clone ID Related Accession (Same CDS sequence)	NM_001244331.1
Accession Version	NM_001244331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Sus scrofa(pig)
Product	neurofilament light polypeptide
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FS640330.1, CK467011.1, CK464912.1, AK352068.1, DY405936.1, DY404591.1, EW194847.2, CK462720.1 and BQ597929.1. On Sep 16, 2011 this sequence version replaced XM_003132813.2. ##Evidence-Data-START## Transcript exon combination :: AK352068.1, EW574888.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAACGCCG CTATGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCTGCTTAC 
TCCAGCTACT CCGCGCCCGT CTCCTCCTCG CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC 
TCCGGCTCCT TGATGCCCAG TCTCGAGAAC CTCGACCTGA GCCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACACAGGAG AAGGCGCAGC TGCAGGACCT CAACGACCGC 
TTTGCCAGCT TCATCGAGCG CGTGCACGAG TTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCGTCCC GCTTCCGGGC GCTGTACGAG 
CAGGAGATCC GTGACCTGCG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTC 
CAGGGCGAGC GCGAGGGGCT GGAGGAGACT CTGCGCAACC TGCAAGCGCG CTACGAAGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGGGCCGAC 
GAGGCGGCTC TCGCCCGCGC CGAGCTCGAA AAGCGCATTG ACAGTCTGAT GGACGAAATC 
GCCTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCTCA GATCCAGTAC 
GCGCAGATCT CCGTGGAGAT GGATGTGTCC TCCAAGCCCG ACCTCTCCGC CGCGCTCAAG 
GACATCCGCG CCCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAACGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACGGT GCTGACCGAG AGCGCCGCCA AGAACACCGA TGCGGTGCGC 
GCAGCCAAGG ACGAGGTGTC CGAGAGCCGC CGACTGCTCA AGGCCAAGAC CCTGGAGATC 
GAAGCCTGCC GGGGCATGAA CGAAGCGCTG GAGAAGCAGC TGCAGGAGCT GGAGGACAAG 
CAGAACGCCG ACATTAGTGC TATGCAGGAC ACAATTAACA AATTAGAAAA CGAACTGAGA 
ACCACAAAGA GTGAAATGGC AAGATACCTG AAGGAATACC AAGACCTTCT CAATGTGAAG 
ATGGCTTTGG ACATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGTGA GGAGACCCGG 
CTGAGTTTCA CCAGCGTGGG AAGCTTAACC ACTGGCTACT CTCAGAGCTC CCAGGTCTTT 
GGCCGATCTG CCTATGGCGG TTTACAGACC AGCTCCTACC TGATGTCCAC CCGCTCCTTC 
CCATCATACT ACACCAGCCA CGTCCAGGAG GAGCAGATCG AGGTGGAGGA GACCATTGAG 
GCTGCGAAAG CTGAGGAAGC CAAGGACGAA CCTCCCTCTG AAGGAGAAGC CGAGGAGGAG 
GGCAAGGAGA AGGAAGAAGC TGAGGCAGAG GCTGAGGCTG AGGAAGAGGA AGGTGCCCAA 
GAAGAAGAAG AAGAAGCTGC CAAGGAAGAA TCTGAGGAAG CAAAGGAGGA AGAAGAAGGA 
GGTGAAGGCG AAGAAGGAGA AGAAACCAAA GAAGCCGAGG AGGAGGAGAA AAAAGATGAA 
GGTGCTGGGG AGGAGCAAGC AACTAAGAAG AAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231260.1
CDS	95..1753
Translation	MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSLTTGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEGKEKEEAEAEAEAEEEEGAQEEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKDEGAGEEQATKKKD

Target ORF information:

RefSeq Version	NM_001244331.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa neurofilament light (NEFL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244331.1

ORF Insert Sequence:

ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAACGCCG CTATGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCTGCTTAC 
TCCAGCTACT CCGCGCCCGT CTCCTCCTCG CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC 
TCCGGCTCCT TGATGCCCAG TCTCGAGAAC CTCGACCTGA GCCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACACAGGAG AAGGCGCAGC TGCAGGACCT CAACGACCGC 
TTTGCCAGCT TCATCGAGCG CGTGCACGAG TTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCGTCCC GCTTCCGGGC GCTGTACGAG 
CAGGAGATCC GTGACCTGCG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTC 
CAGGGCGAGC GCGAGGGGCT GGAGGAGACT CTGCGCAACC TGCAAGCGCG CTACGAAGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGGGCCGAC 
GAGGCGGCTC TCGCCCGCGC CGAGCTCGAA AAGCGCATTG ACAGTCTGAT GGACGAAATC 
GCCTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCTCA GATCCAGTAC 
GCGCAGATCT CCGTGGAGAT GGATGTGTCC TCCAAGCCCG ACCTCTCCGC CGCGCTCAAG 
GACATCCGCG CCCAGTATGA GAAGCTGGCC GCCAAGAACA TGCAGAACGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACGGT GCTGACCGAG AGCGCCGCCA AGAACACCGA TGCGGTGCGC 
GCAGCCAAGG ACGAGGTGTC CGAGAGCCGC CGACTGCTCA AGGCCAAGAC CCTGGAGATC 
GAAGCCTGCC GGGGCATGAA CGAAGCGCTG GAGAAGCAGC TGCAGGAGCT GGAGGACAAG 
CAGAACGCCG ACATTAGTGC TATGCAGGAC ACAATTAACA AATTAGAAAA CGAACTGAGA 
ACCACAAAGA GTGAAATGGC AAGATACCTG AAGGAATACC AAGACCTTCT CAATGTGAAG 
ATGGCTTTGG ACATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGTGA GGAGACCCGG 
CTGAGTTTCA CCAGCGTGGG AAGCTTAACC ACTGGCTACT CTCAGAGCTC CCAGGTCTTT 
GGCCGATCTG CCTATGGCGG TTTACAGACC AGCTCCTACC TGATGTCCAC CCGCTCCTTC 
CCATCATACT ACACCAGCCA CGTCCAGGAG GAGCAGATCG AGGTGGAGGA GACCATTGAG 
GCTGCGAAAG CTGAGGAAGC CAAGGACGAA CCTCCCTCTG AAGGAGAAGC CGAGGAGGAG 
GGCAAGGAGA AGGAAGAAGC TGAGGCAGAG GCTGAGGCTG AGGAAGAGGA AGGTGCCCAA 
GAAGAAGAAG AAGAAGCTGC CAAGGAAGAA TCTGAGGAAG CAAAGGAGGA AGAAGAAGGA 
GGTGAAGGCG AAGAAGGAGA AGAAACCAAA GAAGCCGAGG AGGAGGAGAA AAAAGATGAA 
GGTGCTGGGG AGGAGCAAGC AACTAAGAAG AAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags.
Genome biology8(4)R45(2007)
Gorodkin J,Cirera S,Hedegaard J,Gilchrist MJ,Panitz F,Jørgensen C,Scheibye-Knudsen K,Arvin T,Lumholdt S,Sawera M,Green T,Nielsen BJ,Havgaard JH,Rosenkilde C,Wang J,Li H,Li R,Liu B,Hu S,Dong W,Li W,Yu J,Wang J,Staefeldt HH,Wernersson R,Madsen LB,Thomsen B,Hornshøj H,Bujie Z,Wang X,Wang X,Bolund L,Brunak S,Yang H,Bendixen C,Fredholm M

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

Specific recognition of coiled coils by infrared spectroscopy: analysis of the three structural domains of type III intermediate filament proteins.
Biochemistry35(5)1375-82(1996 Feb)
Heimburg T,Schuenemann J,Weber K,Geisler N

The complete amino acid sequence of the major mammalian neurofilament protein (NF-L).
FEBS letters182(2)475-8(1985 Mar)
Geisler N,Plessmann U,Weber K

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NEFL cDNA ORF clone, Sus scrofa(pig)

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Target ORF information:

ORF Insert Sequence:

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