ORF » Species Summary » Strongylocentrotus purpuratus » LOC580005 cDNA ORF clone

LOC580005 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

The following LOC580005 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC580005 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSd14256 XM_003729029.2
Latest version!		 Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OSd22981 XM_011684152.1
Latest version!		 Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OSd50706 XM_003729029.3
Latest version!		 Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC580005 ( XM_003729029.2 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_003729077.1 Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OSd14256
    Clone ID Related Accession (Same CDS sequence) XM_003729029.2
    Accession Version XM_003729029.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1427040000000
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Product sphingosine kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011969427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_003729029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCGAGTA CAGATGCACT TCCCCAATTT TCAGCTGTTG AGGATATTCT TACGCAGGCT 
    ACATTCCATC TGAAAATTGG GAAACATTCT TTTGATGTTG ATGTCTCAAT ATGTCGCGAA 
    AAGCTTACAT ATCGGAGCGT AAATGGAGCC AAATTGAAAG GAGAAAGCAT AGCATTCACT 
    GACATTGTGG GATGCCGAAG CATCGGGGGA GGTGTCTGCC AGCAGTCGTC GACGGATAGC 
    GGGGACGGTG TGCCCAGAAA AAAGGAAACC AATCATAAAT TGAAATTAAG CGAGAAGAGT 
    GTATGCTTGC AGATTTTTGC ATACAAGTAT AACAAAAATC CCAAAGCTGG TGGCGTGAGA 
    CGCAGAGTAG AGCTAAATTT TGTGACAGAT GAGTTTGAAA CACAAAAGGA GAATGCTTCA 
    TTGGTGGAGA AATGGCGTTT GATTATTCTG GCTCTTCTTC AGGGAGTTGA GTTTTCCAGT 
    GTAGATGACT TGACGGAGTC CCTTATACCA CCACCACCGC ACTACTTGGT ATGCATCAAT 
    CCATTCAGTG GAAAAGAGAA AGCAGTTCAA CTCTTTAAGG AACAAGCCAA GCCTATATTT 
    GAGGAGGCCG GAATCACATT TAAAGAAATC ATAACAGAGA GAAGAGGACA TGGTACTGAA 
    ATAGCTATGA ACCTTGACCT GAAGGAGTAC AATGGGGTCA TCATTGTCTC AGGTGATGGC 
    CTTTTTTATG AGTTCATCAA TGGGTTTGGA CAAAGAAGGG ACCGAGAAGA AGCGTTTAAG 
    ATGCCTCTGG GAATCCTCCC AGGGGGTTCT GGAAACGCAC TTTGTTCAGC AGTGTTGGTA 
    AACAGAGGGG AGCAAGTATT AAAGAACATG GCCTGCCATG CTGCCGTAGC CATTGTCAAG 
    GGCAAAGTCA ACCCCAAGGA CATGGTACAG ATCCAGACCC AAAATGAGAC GGTACTTTCC 
    TTTCTTTCTG TGGCCTGGGG CATCCTAGCT GACATCGACA TAGAGAGTGA GAGGTTCCGT 
    TTCCTAGGGG CAACAAGGTT TCAGGCCCAG GCAGTCCAGA GGATCATGTT CCTCCGCAAG 
    ACTCCAGGAC GCCTGTCATT TCTACCCATC AAGGACGAGT CCAAGTATCG TCACCTCTGG 
    GGAGCAGAGG ACAAGAAAAG GGATAGCTCA AATGGCATCA GCATACAGGA AAGCCAGCAG 
    ATAGAAACCA ACATCGGGGC AAAGATAGGG GACTCCAGGA GCAGCAGTGC CAACCAGAAT 
    GCAAACGCAA GTTCATCCGA GGAAGGTACC TCAAATGCAA TGGGTGAGGT GCATGCAGAT 
    GATCTGGCGA TGCCTTCGCT GAGCGATCCG GTTCCTCCAA ACTGGACAGT CATTCAAGGA 
    GATTTTGTTT GTGTCCTCAT AACCATTGTG TCCCACATAT CCAATGACTT CATGAGCCAT 
    CCTGGTCGGT CAATGGATGA GGGCATCATC ATGGTCCAGT ATATGGACGA GACAACAACA 
    CGCTGGAAAC TGATAAACAC CTTCGATAAA TACATGACTG GGGAGTACCT TAAACAGGAC 
    TTTGTCAAGG CAAGAGTAGT GAAGGCTTTC AGGCTTGAGC CACTTAATAC CAAGGAGGGT 
    ATCTTGACTG TAGATGGAGA GCAGGTCAAG TTTGGACCCA TACAAGGCAT GTTGACCCCA 
    TCAAAATGTC ACTGTATAAT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003729077.1
    CDS40..1743
    Translation

    Target ORF information:

    RefSeq Version XM_003729029.2
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Definition Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003729029.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCGAGTA CAGATGCACT TCCCCAATTT TCAGCTGTTG AGGATATTCT TACGCAGGCT 
    ACATTCCATC TGAAAATTGG GAAACATTCT TTTGATGTTG ATGTCTCAAT ATGTCGCGAA 
    AAGCTTACAT ATCGGAGCGT AAATGGAGCC AAATTGAAAG GAGAAAGCAT AGCATTCACT 
    GACATTGTGG GATGCCGAAG CATCGGGGGA GGTGTCTGCC AGCAGTCGTC GACGGATAGC 
    GGGGACGGTG TGCCCAGAAA AAAGGAAACC AATCATAAAT TGAAATTAAG CGAGAAGAGT 
    GTATGCTTGC AGATTTTTGC ATACAAGTAT AACAAAAATC CCAAAGCTGG TGGCGTGAGA 
    CGCAGAGTAG AGCTAAATTT TGTGACAGAT GAGTTTGAAA CACAAAAGGA GAATGCTTCA 
    TTGGTGGAGA AATGGCGTTT GATTATTCTG GCTCTTCTTC AGGGAGTTGA GTTTTCCAGT 
    GTAGATGACT TGACGGAGTC CCTTATACCA CCACCACCGC ACTACTTGGT ATGCATCAAT 
    CCATTCAGTG GAAAAGAGAA AGCAGTTCAA CTCTTTAAGG AACAAGCCAA GCCTATATTT 
    GAGGAGGCCG GAATCACATT TAAAGAAATC ATAACAGAGA GAAGAGGACA TGGTACTGAA 
    ATAGCTATGA ACCTTGACCT GAAGGAGTAC AATGGGGTCA TCATTGTCTC AGGTGATGGC 
    CTTTTTTATG AGTTCATCAA TGGGTTTGGA CAAAGAAGGG ACCGAGAAGA AGCGTTTAAG 
    ATGCCTCTGG GAATCCTCCC AGGGGGTTCT GGAAACGCAC TTTGTTCAGC AGTGTTGGTA 
    AACAGAGGGG AGCAAGTATT AAAGAACATG GCCTGCCATG CTGCCGTAGC CATTGTCAAG 
    GGCAAAGTCA ACCCCAAGGA CATGGTACAG ATCCAGACCC AAAATGAGAC GGTACTTTCC 
    TTTCTTTCTG TGGCCTGGGG CATCCTAGCT GACATCGACA TAGAGAGTGA GAGGTTCCGT 
    TTCCTAGGGG CAACAAGGTT TCAGGCCCAG GCAGTCCAGA GGATCATGTT CCTCCGCAAG 
    ACTCCAGGAC GCCTGTCATT TCTACCCATC AAGGACGAGT CCAAGTATCG TCACCTCTGG 
    GGAGCAGAGG ACAAGAAAAG GGATAGCTCA AATGGCATCA GCATACAGGA AAGCCAGCAG 
    ATAGAAACCA ACATCGGGGC AAAGATAGGG GACTCCAGGA GCAGCAGTGC CAACCAGAAT 
    GCAAACGCAA GTTCATCCGA GGAAGGTACC TCAAATGCAA TGGGTGAGGT GCATGCAGAT 
    GATCTGGCGA TGCCTTCGCT GAGCGATCCG GTTCCTCCAA ACTGGACAGT CATTCAAGGA 
    GATTTTGTTT GTGTCCTCAT AACCATTGTG TCCCACATAT CCAATGACTT CATGAGCCAT 
    CCTGGTCGGT CAATGGATGA GGGCATCATC ATGGTCCAGT ATATGGACGA GACAACAACA 
    CGCTGGAAAC TGATAAACAC CTTCGATAAA TACATGACTG GGGAGTACCT TAAACAGGAC 
    TTTGTCAAGG CAAGAGTAGT GAAGGCTTTC AGGCTTGAGC CACTTAATAC CAAGGAGGGT 
    ATCTTGACTG TAGATGGAGA GCAGGTCAAG TTTGGACCCA TACAAGGCAT GTTGACCCCA 
    TCAAAATGTC ACTGTATAAT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC580005 ( XM_011684152.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011682454.1 Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OSd22981
    Clone ID Related Accession (Same CDS sequence) XM_011684152.1
    Accession Version XM_011684152.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1427040000000
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Product sphingosine kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011969427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCGAGTA CAGATGCACT TCCCCAATTT TCAGCTGTTG AGGATATTCT TACGCAGGCT 
    ACATTCCATC TGAAAATTGG GAAACATTCT TTTGATGTTG ATGTCTCAAT ATGTCGCGAA 
    AAGCTTACAT ATCGGAGCGT AAATGGAGCC AAATTGAAAG GAGAAAGCAT AGCATTCACT 
    GACATTGTGG GATGCCGAAG CATCGGGGGA GGTGTCTGCC AGCAGTCGTC GACGGATAGC 
    GGGGACGGTG TGCCCAGAAA AAAGGAAACC AATCATAAAT TGAAATTAAG CGAGAAGAGT 
    GTATGCTTGC AGATTTTTGC ATACAAGTAT AACAAAAATC CCAAAGCTGG TGGCGTGAGA 
    CGCAGAGTAG AGCTAAATTT TGTGACAGAT GAGTTTGAAA CACAAAAGGA GAATGCTTCA 
    TTGGTGGAGA AATGGCGTTT GATTATTCTG GCTCTTCTTC AGGGAGTTGA GTTTTCCAGT 
    GTAGATGACT TGACGGAGTC CCTTATACCA CCACCACCGC ACTACTTGGT ATGCATCAAT 
    CCATTCAGTG GAAAAGAGAA AGCAGTTCAA CTCTTTAAGG AACAAGCCAA GCCTATATTT 
    GAGGAGGCCG GAATCACATT TAAAGAAATC ATAACAGAGA GAAGAGGACA TGGTACTGAA 
    ATAGCTATGA ACCTTGACCT GAAGGAGTAC AATGGGGTCA TCATTGTCTC AGGTGATGGC 
    CTTTTTTATG AGTTCATCAA TGGGTTTGGA CAAAGAAGGG ACAGAGAAGA AGCGTTTAAG 
    ATGCCTCTGG GAATCCTCCC AGGGGGTTCT GGAAACGCAC TTTGTTCAGC AGTGTTGGTA 
    AACAGAGGGG AGCAAGTATT AAAGAACATG GCCTGCCATG CTGCCGTAGC CATTGTCAAG 
    GGCAAAGTCA ACCCCAAGGA CATGGTACAG ATCCAGACCC AAAATGAGAC GGTACTTTCC 
    TTTCTTTCTG TGGCCTGGGG CATCCTAGCT GACATCGACA TAGAGAGTGA GAGGTTCCGT 
    TTCCTAGGGG CAACAAGGTT TCAGGCCCAG GCAGTCCAGA GGATCATGTT CCTCCGCAAG 
    ACTCCAGGAC GCCTGTCATT TCTACCCATC AAGGACGAGT CCAAGTATCG TCACCTCTGG 
    GGAGCAGAGG ACAAGAAAAG GGATAGCTCA AATGGCATCA GCATACAGGA AAGCCAGCAG 
    ATAGAAACCA ACATCGGGGC AAAGATAGGG GACTCCAGGA GCAGCAGTGC CAACCAGAAT 
    GCAAACGCAA GTTCATCCGA GGAAGGTACC TCAAATGCAA TGGGTGAGGT GCATGCAGAT 
    GATCTGGCGA TGCCTTCGCT GAGCGATCCG GTTCCTCCAA ACTGGACAGT CATTCAAGGA 
    GATTTTGTTT GTGTCCTCAT AACCATTGTG TCCCACATAT CCAATGACTT CATGAGCCAT 
    CCTGGTCGGT CAATGGATGA GGGCATCATC ATGGTCCAGT ATATGGACGA GACAACAACA 
    CGCTGGAAAC TGATAAACAC CTTCGATAAA TACATGACTG GGGAGTACCT TAAACAGGAC 
    TTTGTCAAGG CAAGAGTAGT GAAGGCTTTC AGGCTTGAGC CACTTAATAC CAAGGAGGGT 
    ATCTTGACTG TAGATGGAGA GCAGGTCAAG TTTGGACCCA TACAAGGCAT GTTGACCCCA 
    TCAAAATGTC ACTGTATAAT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011682454.1
    CDS40..1743
    Translation

    Target ORF information:

    RefSeq Version XM_011684152.1
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Definition Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011684152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCGAGTA CAGATGCACT TCCCCAATTT TCAGCTGTTG AGGATATTCT TACGCAGGCT 
    ACATTCCATC TGAAAATTGG GAAACATTCT TTTGATGTTG ATGTCTCAAT ATGTCGCGAA 
    AAGCTTACAT ATCGGAGCGT AAATGGAGCC AAATTGAAAG GAGAAAGCAT AGCATTCACT 
    GACATTGTGG GATGCCGAAG CATCGGGGGA GGTGTCTGCC AGCAGTCGTC GACGGATAGC 
    GGGGACGGTG TGCCCAGAAA AAAGGAAACC AATCATAAAT TGAAATTAAG CGAGAAGAGT 
    GTATGCTTGC AGATTTTTGC ATACAAGTAT AACAAAAATC CCAAAGCTGG TGGCGTGAGA 
    CGCAGAGTAG AGCTAAATTT TGTGACAGAT GAGTTTGAAA CACAAAAGGA GAATGCTTCA 
    TTGGTGGAGA AATGGCGTTT GATTATTCTG GCTCTTCTTC AGGGAGTTGA GTTTTCCAGT 
    GTAGATGACT TGACGGAGTC CCTTATACCA CCACCACCGC ACTACTTGGT ATGCATCAAT 
    CCATTCAGTG GAAAAGAGAA AGCAGTTCAA CTCTTTAAGG AACAAGCCAA GCCTATATTT 
    GAGGAGGCCG GAATCACATT TAAAGAAATC ATAACAGAGA GAAGAGGACA TGGTACTGAA 
    ATAGCTATGA ACCTTGACCT GAAGGAGTAC AATGGGGTCA TCATTGTCTC AGGTGATGGC 
    CTTTTTTATG AGTTCATCAA TGGGTTTGGA CAAAGAAGGG ACAGAGAAGA AGCGTTTAAG 
    ATGCCTCTGG GAATCCTCCC AGGGGGTTCT GGAAACGCAC TTTGTTCAGC AGTGTTGGTA 
    AACAGAGGGG AGCAAGTATT AAAGAACATG GCCTGCCATG CTGCCGTAGC CATTGTCAAG 
    GGCAAAGTCA ACCCCAAGGA CATGGTACAG ATCCAGACCC AAAATGAGAC GGTACTTTCC 
    TTTCTTTCTG TGGCCTGGGG CATCCTAGCT GACATCGACA TAGAGAGTGA GAGGTTCCGT 
    TTCCTAGGGG CAACAAGGTT TCAGGCCCAG GCAGTCCAGA GGATCATGTT CCTCCGCAAG 
    ACTCCAGGAC GCCTGTCATT TCTACCCATC AAGGACGAGT CCAAGTATCG TCACCTCTGG 
    GGAGCAGAGG ACAAGAAAAG GGATAGCTCA AATGGCATCA GCATACAGGA AAGCCAGCAG 
    ATAGAAACCA ACATCGGGGC AAAGATAGGG GACTCCAGGA GCAGCAGTGC CAACCAGAAT 
    GCAAACGCAA GTTCATCCGA GGAAGGTACC TCAAATGCAA TGGGTGAGGT GCATGCAGAT 
    GATCTGGCGA TGCCTTCGCT GAGCGATCCG GTTCCTCCAA ACTGGACAGT CATTCAAGGA 
    GATTTTGTTT GTGTCCTCAT AACCATTGTG TCCCACATAT CCAATGACTT CATGAGCCAT 
    CCTGGTCGGT CAATGGATGA GGGCATCATC ATGGTCCAGT ATATGGACGA GACAACAACA 
    CGCTGGAAAC TGATAAACAC CTTCGATAAA TACATGACTG GGGAGTACCT TAAACAGGAC 
    TTTGTCAAGG CAAGAGTAGT GAAGGCTTTC AGGCTTGAGC CACTTAATAC CAAGGAGGGT 
    ATCTTGACTG TAGATGGAGA GCAGGTCAAG TTTGGACCCA TACAAGGCAT GTTGACCCCA 
    TCAAAATGTC ACTGTATAAT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC580005 ( XM_003729029.3 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_003729077.2 Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), mRNA.
    Price & Availability↑
    CloneID OSd50706
    Clone ID Related Accession (Same CDS sequence) XM_003729029.3
    Accession Version XM_003729029.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1569427200000
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Product sphingosine kinase 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145599.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_003729029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCGAGTA CAGATGCACT TCCCCAATTT TCAGCTGTTG AGGATATTCT TACGCAGGCT 
    ACATTCCATC TGAAAATTGG GAAACATTCT TTTGATGTTG ATGTCTCAAT ATGTCGCGAA 
    AAGCTTACAT ATCGGAGCGT AAATGGAGCC AAATTGAAAG GAGAAAGCAT AGCATTCACT 
    GACATTGTGG GATGCCGAAG CATCGGGGGA GGTGTCGGCC AGCAGTCGTC GACGGATAGC 
    GGGGACGGTG TGCCCAGAAA AAAGGAACTC AATCATAAAT TGAAATTAAG CGAGAAGAGT 
    GCATGCTTGC AGATTTTTGC ATACAAGTAT AACAAAAATC CCAATGCTGG TGGCGTGAGA 
    CGCAGAGTAG AGCTAAATTT TGTGACAGAT GAGTTTGAAA CACAAAAGGA GAATGCTTCA 
    TTGGTGGAGA AATGGCGTTT GATTATTCTG GCTCTTCTTC AGGGAGTTGA GTTTTCCAGT 
    GTAGATGACT TGACGGAGTC CCTTATACCA CCACCACCGC ACTACTTGGT ATGCATCAAT 
    CCATTCAGTG GAAAAGAGAA AGCAGTTCAA CTCTTTAAGG AACAAGCCAA GCCTATATTT 
    GAGGAGGCCG GAATCACATT TAAAGAAATC ATAACAGAGA GAAGAGGACA TGGTACTGAA 
    ATAGCTATGA ACCTTGACCT GAAGGAGTAC AATGGGGTCA TCATTGTCTC AGGTGATGGC 
    CTTTTTTATG AGTTCATCAA TGGGTTTGGA CAAAGAAGGG ACCGAGAAGA AGCGTTTAAG 
    ATGCCTCTGG GAATCCTCCC AGGGGGTTCT GGAAACGCAC TTTGTTCAGC AGTGTTGGTA 
    AACAGAGGGG AGCAAGTATT AAAGAACATG GCCTGCCATG CTGCCGTAGC CATTGTCAAG 
    GGCAAAGTCA ACCCCAAGGA CATGGTACAG ATCCAGACCC AAAATGAGAC GGTACTTTCC 
    TTTCTTTCTG TGGCCTGGGG CATCCTAGCT GACATCGACA TAGAGAGTGA GAGGTTCCGT 
    TTCCTAGGGG CAACAAGGTT TCAGGCCCAG GCAGTCCAGA GGATCATGTT CCTCCGCAAG 
    AATCCAGGAC GCCTGTCATT TCTACCCATC AAGGACGAGT CCAAGTATCA TCACCTCTGG 
    GGAGCAGAGG ACAAGAAAAG GGATAGCTCA AGTGGCATCA GCATACAGGA AAGCCAGCAG 
    ATAGAAACCA ACATCGGGGC AAAGATGGGG GACTCCAGGA GCAGCAGTGC CAACCAGAAT 
    GCAAACGCAA GTTCATCCGA GGAAGGTACC TCAAATGCAA TGGGTGAGGT GCATGCAGAT 
    GATCTGGCGA TGCCTTCGCT GAGCGATCCG GTTCCTCCAA ACTGGACAGT CATTCAAGGA 
    GATTTTGTTT GTGTCCTCAT AACCATTGTG TCCCACATAT CCAATGACTT CATGAGCCAT 
    CCTGGTCGGT CAATGGATGA GGGCATCATC ATGGTCCAGT ATATGGACGA GACAACAACA 
    CGCTGGAAAC TGATAAACAC CTTCGATAAA TACATGACTG GGGAGTACCT TAAACAGGAC 
    TTTGTCAAGG CAAGAGTAGT GAAGGCTTTC AGGCTTGAGC CACTTAATAC CAAGGAGGGT 
    ATCTTGACTG TAGATGGAGA GCAGGTCAAG TTTGGACCCA TACAAGGCAT GTTGACCCCA 
    TCAAAATGTC ACTGTATAAT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003729077.2
    CDS45..1748
    Translation

    Target ORF information:

    RefSeq Version XM_003729029.3
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Definition Strongylocentrotus purpuratus sphingosine kinase 2 (LOC580005), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003729029.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCGAGTA CAGATGCACT TCCCCAATTT TCAGCTGTTG AGGATATTCT TACGCAGGCT 
    ACATTCCATC TGAAAATTGG GAAACATTCT TTTGATGTTG ATGTCTCAAT ATGTCGCGAA 
    AAGCTTACAT ATCGGAGCGT AAATGGAGCC AAATTGAAAG GAGAAAGCAT AGCATTCACT 
    GACATTGTGG GATGCCGAAG CATCGGGGGA GGTGTCGGCC AGCAGTCGTC GACGGATAGC 
    GGGGACGGTG TGCCCAGAAA AAAGGAACTC AATCATAAAT TGAAATTAAG CGAGAAGAGT 
    GCATGCTTGC AGATTTTTGC ATACAAGTAT AACAAAAATC CCAATGCTGG TGGCGTGAGA 
    CGCAGAGTAG AGCTAAATTT TGTGACAGAT GAGTTTGAAA CACAAAAGGA GAATGCTTCA 
    TTGGTGGAGA AATGGCGTTT GATTATTCTG GCTCTTCTTC AGGGAGTTGA GTTTTCCAGT 
    GTAGATGACT TGACGGAGTC CCTTATACCA CCACCACCGC ACTACTTGGT ATGCATCAAT 
    CCATTCAGTG GAAAAGAGAA AGCAGTTCAA CTCTTTAAGG AACAAGCCAA GCCTATATTT 
    GAGGAGGCCG GAATCACATT TAAAGAAATC ATAACAGAGA GAAGAGGACA TGGTACTGAA 
    ATAGCTATGA ACCTTGACCT GAAGGAGTAC AATGGGGTCA TCATTGTCTC AGGTGATGGC 
    CTTTTTTATG AGTTCATCAA TGGGTTTGGA CAAAGAAGGG ACCGAGAAGA AGCGTTTAAG 
    ATGCCTCTGG GAATCCTCCC AGGGGGTTCT GGAAACGCAC TTTGTTCAGC AGTGTTGGTA 
    AACAGAGGGG AGCAAGTATT AAAGAACATG GCCTGCCATG CTGCCGTAGC CATTGTCAAG 
    GGCAAAGTCA ACCCCAAGGA CATGGTACAG ATCCAGACCC AAAATGAGAC GGTACTTTCC 
    TTTCTTTCTG TGGCCTGGGG CATCCTAGCT GACATCGACA TAGAGAGTGA GAGGTTCCGT 
    TTCCTAGGGG CAACAAGGTT TCAGGCCCAG GCAGTCCAGA GGATCATGTT CCTCCGCAAG 
    AATCCAGGAC GCCTGTCATT TCTACCCATC AAGGACGAGT CCAAGTATCA TCACCTCTGG 
    GGAGCAGAGG ACAAGAAAAG GGATAGCTCA AGTGGCATCA GCATACAGGA AAGCCAGCAG 
    ATAGAAACCA ACATCGGGGC AAAGATGGGG GACTCCAGGA GCAGCAGTGC CAACCAGAAT 
    GCAAACGCAA GTTCATCCGA GGAAGGTACC TCAAATGCAA TGGGTGAGGT GCATGCAGAT 
    GATCTGGCGA TGCCTTCGCT GAGCGATCCG GTTCCTCCAA ACTGGACAGT CATTCAAGGA 
    GATTTTGTTT GTGTCCTCAT AACCATTGTG TCCCACATAT CCAATGACTT CATGAGCCAT 
    CCTGGTCGGT CAATGGATGA GGGCATCATC ATGGTCCAGT ATATGGACGA GACAACAACA 
    CGCTGGAAAC TGATAAACAC CTTCGATAAA TACATGACTG GGGAGTACCT TAAACAGGAC 
    TTTGTCAAGG CAAGAGTAGT GAAGGCTTTC AGGCTTGAGC CACTTAATAC CAAGGAGGGT 
    ATCTTGACTG TAGATGGAGA GCAGGTCAAG TTTGGACCCA TACAAGGCAT GTTGACCCCA 
    TCAAAATGTC ACTGTATAAT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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