LOC8062510 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8062510
Entrez Gene ID	8062510
Full Name	calcium-transporting ATPase 3, plasma membrane-type
Gene Type	protein-coding
Organism	Sorghum bicolor(sorghum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002466773.1	XP_002466818.1	hypothetical protein
XM_002466773.2	XP_002466818.2	calcium-transporting ATPase 3, plasma membrane-type

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Sorghum bicolor genome and the diversification of grasses.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KF, Messing J, Rokhsar DS
Nature457(7229)551-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC8062510 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8062510 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc12028	XM_002466773.1 Latest version!	Sorghum bicolor hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSc108940	XM_002466773.2 Latest version!	Sorghum bicolor calcium-transporting ATPase 3, plasma membrane-type (LOC8062510), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8062510 ( XM_002466773.1 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002466818.1 Sorghum bicolor hypothetical protein, mRNA.

Price & Availability↑

CloneID	OSc12028
Clone ID Related Accession (Same CDS sequence)	XM_002466773.1
Accession Version	XM_002466773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1247328000000
Organism	Sorghum bicolor(Sorghum)
Product	hypothetical protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012870). COMPLETENESS: incomplete on both ends.

ATGTCGGCCG CGGCGGGGAC GCCACCGGAA GGCGGAGGGG ATCGGCCGTG GCAGTCTTAC 
CATACCGCAT ACACCAACGC CAAAGCTGGA ATGGAGGGTG TTGACAAAGA GAAGGTGCAG 
AAGGTAATCT ATGAAATGAG CAAGGGCTCA AAATATTTCG AAAATGAGCA GAGGAAGGAG 
GCAGTGACCA AACAAAAGAT TGAACATCTC CGTGCACAAT GTGCAGCATT GACTGATAAT 
GACATATCAC ATTTTCAGAA GGTTGCTGAG AAGAAAATGT TAGAGTTGGA AGCTTCACGA 
GATCTTTCAA AGATATGGCT TCACACTGAC ATGGATGCCT TTTATGCTGC TGTTGAGACA 
TTGGAGAATC CATCCTTGAA GGGAAAACCC TTGGCGGTTG GTAGCATGTC TATGATAGCT 
ACTGCCAGTT ATGAGGCACG TAAGTTTGGA GTGCGTGCAG CGATGCCTGG TTTTATTGGA 
TGCAAACTGT GTCCTGGCTT GGTTTTTGTC CGACCAAACT TTGAGAGATA TACTCACTAT 
AGCGAGCTGA CAAGAAAAGT TTTCCAGAGG TATGATCCCA ATTTCATTGC AACAAGTCTA 
GATGAAGCAT ACCTCAACAT AACAAATGTT TGCATTGAGA GAGGCATTAC AGGCGAGGAG 
GTTGCTACTG AACTTAGGTC TGCCATTTAC CAGGAAACTG GTCTAACATG TAGTGCTGGG 
GTTGCTCCAA ACCGTATGAT TGCAAAGGTT TGCTCTGATA TTAACAAGCC CAATGGGCAG 
TTTATTTTGC CAAATGACCG GGAAGCTGTA TTGACATTTG TATCTACATT GCCTATAAGA 
AAGATAGGTG GCATAGGTAA GGTCACAGAA CAGATGTTAC GTCAGGTTCT TGGAATCAGT 
ACATGCCAGG AGATGCTGCA AAAGGCTGCT TTTTTGTGTG CTCTTTTTTC GGAAGGCTCA 
GCTGATTTCT TTCTATCGGT TGGTCTTGGG TTAGGAGGTA CAGAAACCCC GGAGCAAAGG 
CAAAGGAAAA GTATCAGTTG CGAGCGAACT TTCGCAGCAA CAAATGATTC TTCTTTGCTT 
TTCGAAAAAT TAGATAGCCT TGCAGAAAAT TTGACTGATG ATCTGCAAAA GGAGGGTTTG 
AAGGGAAAGA CACTAACACT CAAACTGAAG ACTGCAGATT TTGAGGTTCG TACAAGGGCA 
GTAACTACAC GGAACTACAT CAACTCCAAG GAAGATATCC TGGTCTATGC TACAAAGTTG 
CTTAAGGCTG AAATGCCTCT TTCTTTGAGA CTGATGGGGT TGCGGATGTC TCAGCTACAT 
GATGAGAAGG ATGATCCATC TACTTCAACA CAAAAGACAC TAGATATATT TTTTCGTTCA 
TCAAACGGTA ACTCAAATGT CAATGGAAAT GTTCAGAGAA TCACTAATAC CTCTGGGCAG 
GATAATGATT CTATTAGTCT CACAACAAAG GAATACTTGG TACCTGATGC TGGAACAGGT 
GTTTCCACAG ATCAACAGGA TTTCTTCCTA CATGATGAGA GCTGTTTTAT TCCCGAACAA 
AGGAGTTTTG GCAATCATAG TAATGAGGCT GTCATAAGCA AGCCGTTGGG TGGAACAAAG 
TTAGATGATG TGTCTTCCAG TGCAAAGGTT GCGCCTGCTG AGAAGTTGAA TGAACCTGAT 
AACTTGATCA GTCCGAAGGC TGTGGCTTCA TCAAGCAAAC CTGATCAGCA ATCATGGATT 
GATGGCTACA TTTGTTCCAT TTGTGGATTT GAACTACCTC CAGGCTTTGA GGAGGAGAGA 
CTAGAACATT CAGATTTTCA CTTGGCTGAA ACACTCCAGC AGGAAGAAGC AGTGGACGGT 
ACAAGACACA TCTCAAATGA GAGGCTGGCT GACAGAGAGA GACCATGCTC AACTACACCC 
ACACCCAAGA AGAAACTGAA GTCTTCTAAA GAAGGAAAAC ATATTCCGAT TGACGCTTTC 
TTCACAAAGT GTAATAAGAA CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002466818.1
CDS	1..2007
Translation	MSAAAGTPPEGGGDRPWQSYHTAYTNAKAGMEGVDKEKVQKVIYEMSKGSKYFENEQRKEAVTKQKIEHLRAQCAALTDNDISHFQKVAEKKMLELEASRDLSKIWLHTDMDAFYAAVETLENPSLKGKPLAVGSMSMIATASYEARKFGVRAAMPGFIGCKLCPGLVFVRPNFERYTHYSELTRKVFQRYDPNFIATSLDEAYLNITNVCIERGITGEEVATELRSAIYQETGLTCSAGVAPNRMIAKVCSDINKPNGQFILPNDREAVLTFVSTLPIRKIGGIGKVTEQMLRQVLGISTCQEMLQKAAFLCALFSEGSADFFLSVGLGLGGTETPEQRQRKSISCERTFAATNDSSLLFEKLDSLAENLTDDLQKEGLKGKTLTLKLKTADFEVRTRAVTTRNYINSKEDILVYATKLLKAEMPLSLRLMGLRMSQLHDEKDDPSTSTQKTLDIFFRSSNGNSNVNGNVQRITNTSGQDNDSISLTTKEYLVPDAGTGVSTDQQDFFLHDESCFIPEQRSFGNHSNEAVISKPLGGTKLDDVSSSAKVAPAEKLNEPDNLISPKAVASSSKPDQQSWIDGYICSICGFELPPGFEEERLEHSDFHLAETLQQEEAVDGTRHISNERLADRERPCSTTPTPKKKLKSSKEGKHIPIDAFFTKCNKNL

Target ORF information:

RefSeq Version	XM_002466773.1
Organism	Sorghum bicolor(Sorghum)
Definition	Sorghum bicolor hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002466773.1

ORF Insert Sequence:

ATGTCGGCCG CGGCGGGGAC GCCACCGGAA GGCGGAGGGG ATCGGCCGTG GCAGTCTTAC 
CATACCGCAT ACACCAACGC CAAAGCTGGA ATGGAGGGTG TTGACAAAGA GAAGGTGCAG 
AAGGTAATCT ATGAAATGAG CAAGGGCTCA AAATATTTCG AAAATGAGCA GAGGAAGGAG 
GCAGTGACCA AACAAAAGAT TGAACATCTC CGTGCACAAT GTGCAGCATT GACTGATAAT 
GACATATCAC ATTTTCAGAA GGTTGCTGAG AAGAAAATGT TAGAGTTGGA AGCTTCACGA 
GATCTTTCAA AGATATGGCT TCACACTGAC ATGGATGCCT TTTATGCTGC TGTTGAGACA 
TTGGAGAATC CATCCTTGAA GGGAAAACCC TTGGCGGTTG GTAGCATGTC TATGATAGCT 
ACTGCCAGTT ATGAGGCACG TAAGTTTGGA GTGCGTGCAG CGATGCCTGG TTTTATTGGA 
TGCAAACTGT GTCCTGGCTT GGTTTTTGTC CGACCAAACT TTGAGAGATA TACTCACTAT 
AGCGAGCTGA CAAGAAAAGT TTTCCAGAGG TATGATCCCA ATTTCATTGC AACAAGTCTA 
GATGAAGCAT ACCTCAACAT AACAAATGTT TGCATTGAGA GAGGCATTAC AGGCGAGGAG 
GTTGCTACTG AACTTAGGTC TGCCATTTAC CAGGAAACTG GTCTAACATG TAGTGCTGGG 
GTTGCTCCAA ACCGTATGAT TGCAAAGGTT TGCTCTGATA TTAACAAGCC CAATGGGCAG 
TTTATTTTGC CAAATGACCG GGAAGCTGTA TTGACATTTG TATCTACATT GCCTATAAGA 
AAGATAGGTG GCATAGGTAA GGTCACAGAA CAGATGTTAC GTCAGGTTCT TGGAATCAGT 
ACATGCCAGG AGATGCTGCA AAAGGCTGCT TTTTTGTGTG CTCTTTTTTC GGAAGGCTCA 
GCTGATTTCT TTCTATCGGT TGGTCTTGGG TTAGGAGGTA CAGAAACCCC GGAGCAAAGG 
CAAAGGAAAA GTATCAGTTG CGAGCGAACT TTCGCAGCAA CAAATGATTC TTCTTTGCTT 
TTCGAAAAAT TAGATAGCCT TGCAGAAAAT TTGACTGATG ATCTGCAAAA GGAGGGTTTG 
AAGGGAAAGA CACTAACACT CAAACTGAAG ACTGCAGATT TTGAGGTTCG TACAAGGGCA 
GTAACTACAC GGAACTACAT CAACTCCAAG GAAGATATCC TGGTCTATGC TACAAAGTTG 
CTTAAGGCTG AAATGCCTCT TTCTTTGAGA CTGATGGGGT TGCGGATGTC TCAGCTACAT 
GATGAGAAGG ATGATCCATC TACTTCAACA CAAAAGACAC TAGATATATT TTTTCGTTCA 
TCAAACGGTA ACTCAAATGT CAATGGAAAT GTTCAGAGAA TCACTAATAC CTCTGGGCAG 
GATAATGATT CTATTAGTCT CACAACAAAG GAATACTTGG TACCTGATGC TGGAACAGGT 
GTTTCCACAG ATCAACAGGA TTTCTTCCTA CATGATGAGA GCTGTTTTAT TCCCGAACAA 
AGGAGTTTTG GCAATCATAG TAATGAGGCT GTCATAAGCA AGCCGTTGGG TGGAACAAAG 
TTAGATGATG TGTCTTCCAG TGCAAAGGTT GCGCCTGCTG AGAAGTTGAA TGAACCTGAT 
AACTTGATCA GTCCGAAGGC TGTGGCTTCA TCAAGCAAAC CTGATCAGCA ATCATGGATT 
GATGGCTACA TTTGTTCCAT TTGTGGATTT GAACTACCTC CAGGCTTTGA GGAGGAGAGA 
CTAGAACATT CAGATTTTCA CTTGGCTGAA ACACTCCAGC AGGAAGAAGC AGTGGACGGT 
ACAAGACACA TCTCAAATGA GAGGCTGGCT GACAGAGAGA GACCATGCTC AACTACACCC 
ACACCCAAGA AGAAACTGAA GTCTTCTAAA GAAGGAAAAC ATATTCCGAT TGACGCTTTC 
TTCACAAAGT GTAATAAGAA CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8062510 ( XM_002466773.2 ) cDNA ORF clone, Sorghum bicolor(sorghum) -> XP_002466818.2 Sorghum bicolor calcium-transporting ATPase 3, plasma membrane-type (LOC8062510), mRNA.

Price & Availability↑

CloneID	OSc108940
Clone ID Related Accession (Same CDS sequence)	XM_002466773.2
Accession Version	XM_002466773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497196800000
Organism	Sorghum bicolor(sorghum)
Product	calcium-transporting ATPase 3, plasma membrane-type
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012870.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 13, 2017 this sequence version replaced XM_002466773.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sorghum bicolor Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCGGCCG CGGCGGGGAC GCCACCGGAA GGCGGAGGGG ATCGGCCGTG GCAGTCTTAC 
CATACCGCAT ACACCAACGC CAAAGCTGGA ATGGAGGGTG TTGACAAAGA GAAGGTGCAG 
AAGGTAATCT ATGAAATGAG CAAGGGCTCA AAATATTTCG AAAATGAGCA GAGGAAGGAG 
GCAGTGACCA AACAAAAGAT TGAACATCTC CGTGCACAAT GTGCAGCATT GACTGATAAT 
GACATATCAC ATTTTCAGAA GGTTGCTGAG AAGAAAATGT TAGAGTTGGA AGCTTCACGA 
GATCTTTCAA AGATATGGCT TCACACTGAC ATGGATGCCT TTTATGCTGC TGTTGAGACA 
TTGGAGAATC CATCCTTGAA GGGAAAACCC TTGGCGGTTG GTAGCATGTC TATGATAGCT 
ACTGCCAGTT ATGAGGCACG TAAGTTTGGA GTGCGTGCAG CGATGCCTGG TTTTATTGGA 
TGCAAACTGT GTCCTGGCTT GGTTTTTGTC CGACCAAACT TTGAGAGATA TACTCACTAT 
AGCGAGCTGA CAAGAAAAGT TTTCCAGAGG TATGATCCCA ATTTCATTGC AACAAGTCTA 
GATGAAGCAT ACCTCAACAT AACAAATGTT TGCATTGAGA GAGGCATTAC AGGCGAGGAG 
GTTGCTACTG AACTTAGGTC TGCCATTTAC CAGGAAACTG GTCTAACATG TAGTGCTGGG 
GTTGCTCCAA ACCGTATGAT TGCAAAGGTT TGCTCTGATA TTAACAAGCC CAATGGGCAG 
TTTATTTTGC CAAATGACCG GGAAGCTGTA TTGACATTTG TATCTACATT GCCTATAAGA 
AAGATAGGTG GCATAGGTAA GGTCACAGAA CAGATGTTAC GTCAGGTTCT TGGAATCAGT 
ACATGCCAGG AGATGCTGCA AAAGGCTGCT TTTTTGTGTG CTCTTTTTTC GGAAGGCTCA 
GCTGATTTCT TTCTATCGGT TGGTCTTGGG TTAGGAGGTA CAGAAACCCC GGAGCAAAGG 
CAAAGGAAAA GTATCAGTTG CGAGCGAACT TTCGCAGCAA CAAATGATTC TTCTTTGCTT 
TTCGAAAAAT TAGATAGCCT TGCAGAAAAT TTGACTGATG ATCTGCAAAA GGAGGGTTTG 
AAGGGAAAGA CACTAACACT CAAACTGAAG ACTGCAGATT TTGAGGTTCG TACAAGGGCA 
GTAACTACAC GGAACTACAT CAACTCCAAG GAAGATATCC TGGTCTATGC TACAAAGTTG 
CTTAAGGCTG AAATGCCTCT TTCTTTGAGA CTGATGGGGT TGCGGATGTC TCAGCTACAT 
GATGAGAAGG ATGATCCATC TACTTCAACA CAAAAGACAC TAGATATATT TTTTCGTTCA 
TCAAACGGTA ACTCAAATGT CAATGGAAAT GTTCAGAGAA TCACTAATAC CTCTGGGCAG 
GATAATGATT CTATTAGTCT CACAACAAAG GAATACTTGG TACCTGATGC TGGAACAGGT 
GTTTCCACAG ATCAACAGGA TTTCTTCCTA CATGATGAGA GCTGTTTTAT TCCCGAACAA 
AGGAGTTTTG GCAATCATAG TAATGAGGCT GTCATAAGCA AGCCGTTGGG TGGAACAAAG 
TTAGATGATG TGTCTTCCAG TGCAAAGGTT GCGCCTGCTG AGAAGTTGAA TGAACCTGAT 
AACTTGATCA GTCCGAAGGC TGTGGCTTCA TCAAGCAAAC CTGATCAGCA ATCATGGATT 
GATGGCTACA TTTGTTCCAT TTGTGGATTT GAACTACCTC CAGGCTTTGA GGAGGAGAGA 
CTAGAACATT CAGATTTTCA CTTGGCTGAA ACACTCCAGC AGGAAGAAGC AGTGGACGGT 
ACAAGACACA TCTCAAATGA GAGGCTGGCT GACAGAGAGA GACCATGCTC AACTACACCC 
ACACCCAAGA AGAAACTGAA GTCTTCTAAA GAAGGAAAAC ATATTCCGAT TGACGCTTTC 
TTCACAAAGT GTAATAAGAA CTTTATCCAG TGCTTGTACC TGTCAGTCCG CCCGCCAGCT 
GCACGTGGTC AGAAAAATTC AGCAAGCACT GGAGAGCGAG AGCAAGAGCG AACTAGGGGA 
GGAAGGGGGA AGACGGCAAT CATGCACAGC GGCGTCGACG CTCGGCCGGG ACCCCTCCTC 
CCCCTCCCAG GCGCATCACC AGCTCCCTGC TCCGGCCGCC GCGTCCCATG GCCGCGGTCG 
GGGTTAGGGG CTGCGCTGCG GCGGCCAAAG AACCTCCACG GCCGCCTCCG CTTCGAGCCC 
CTCCCCGCCG GCGACCTCTG CAAGTGGGCC CACCGTGAGA AGCTACGTGT TGCTGTGCTT 
GTATCGAAGT CCACGCTGCA GAGCGAACAT GGTGTTTCAC TTCAGAATGG GCGTGTTGTC 
CCAGAAGGTG TCAAAGCTGC TGGGTTTCAG ATCTCTGCCG ATGACCTAGC ATCAGTTGTT 
GAAAATCGTG ACGCTGAAAA ATTGACTGCG CATGGCCAGC TGGATGGGAT CGCAGACAAG 
TTGGCGACGT CATTAGCTGA TGGAATCACC ACAGATGAGT GCAGCCTGAA TCAACGGCAG 
GACATGTATG GAGTGAACAA GTTCACGGAG AGTGAGGTCC GCAGCTTATG GGAGTTTGTG 
TGGGAGGCAT TGCAAGATAC TACTCTTGTA ATTCTTCTAG CATGTGCCCT TGTATCTTTT 
GTCGTTGGCG TTGCCACGGA AGGATGGCCA AGTGGTGCCC ATGATGGCAT TGGAATTTTT 
ACAAGTATCC TCTTGGTTGT TTCTGTTACC GCGACGAGCA ATTATCAGCA GTCTTTGCAA 
TTCAGGGATC TGGACAAAGA GAAAAGGAAA ATTTCTATTC AGGTTACCAG AGATGGGTTT 
AGACAAAGGA TATTGATAGA TGATCTTCTT CCTGGTGATG TTGTCCACCT AGCTGTTGGA 
GATCAGGTTC CTGCAGATGG CCTCTTTATT TCAGGGTATT CTGTATTGAT CAACGAGTCC 
AGCCTAACTG GTGAGAGTGA ACCTGTTGTC ATAAATGAAG ATAACCCTTT TCTTTTGTCA 
GGGACTAAAG TCCTAGATGG GTCATGCAAA ATGCTGGTTA CAGCAGTAGG GATGCGAACA 
CAGTGGGGTA AACTAATGGC TGCCATCACT GAAAGTGGGG ATGATGAAAC TCCACTGCAG 
GGTAAACTAA ATGGAGTTGC AAATACTATT GGAAATATCG GTCTGTTTTT TGCCCTTTTA 
ACTTTTGTTA TCCTCTCGCA AGGGTTAGTG GCCCAGAAAT ACGCTGATGG GCTTCTTTTA 
AGCTGGTCTG GAGAAGATGT ACTGGAGATA CTGGAACATT TTTCCATTGC AGTCACAATT 
GTTGTTGTTG CGGTTCCTGA GGGATTGCCT TTAGCAGTGA CATTGAGCCT TGCATTTGCA 
ATGAAGAAGA TGATGAATGA GAAGGCACTG GTACGACAGT TAGCTGCCTG TGAAACTATG 
GGCTCAGCCA CTGTCATCTG CAGTGATAAG ACAGGAACTC TTACAACTAA TCGCATGTCT 
GTCATGAAGG CCTGCATATG TGGAAACATC ATGGAAGTGA CCAATCCACC AGTGCTTTCA 
AGTTTTTCTT CCAAACTCCC AGAATTTGCT TTGCAAATTC TTCTGGAATC CATTTTTAAC 
AACACTGCTG GTGAAGTGGT GATTAACCAA GATGGGAACT GTCAGATACT AGGGACCCCA 
ACTGAGGCTG CTTTGTTGGA TTTTGCATTG TCAATAGGTG GTGACTTCAA AGAAAAACGG 
CAGGAAACTA AGATTGTTAA AGTCGAGCCT TTTAATTCAA CAAAAAAGAG GATGAGCACC 
ATTCTCGAGC TTCCTGGTGG AGGTTACCGT GCACATTGTA AAGGTGCTTC AGAAGTAGTC 
TTGGCTGCCT GTGATAAATT CATAGATGCC AGAGGTACTA TTGTTGCACT TGATAAAACA 
GCTACAAAGA AACTCAGTGA CATCATCGAA ACCTTTTCTA AAGAAGCACT CAGGACACTC 
TGCCTTGCTT ATCGGGAAAT GGATGATAGC TTTTCCATTG ATGAGCAAAT ACCGCTACAA 
GGATACACAT GCATTGGTAT TGTTGGGATC AAAGATCCTG TTCGTCCAGG TGTCAGGCAG 
TCTGTGGCAA CTTGCCGGTC TGCTGGCATT GAGGTTCGAA TGGTTACAGG AGACAACATA 
AATACAGCAA AGGCAATTGC TCGTGAATGC GGTATACTTA CTGAGGATGG TATTGCCATT 
GAAGGTGCTG AGTTCAGGGA GAAAAATCCC AAAGAACTCC TTGAGTTGAT TCCAAAGATG 
CAGGTCCTGG CCCGATCATC ACCACTTGAT AAGCATACAC TTGTGAAACA CCTGCGTACC 
ACATTTAATG AGGTTGTTGC TGTGACTGGT GATGGTACAA ATGACGCACC GGCGCTACGT 
GAGGCAGATA TTGGACTTGC CATGGGAATT GCAGGGACTG AGGTAGCAAA AGAGAGCGCT 
GATGTTGTTA TTCTTGATGA TAATTTCTCC ACTATCGTAA CAGTTGCCAA ATGGGGACGC 
TCCGTTTATG TCAACATTCA AAAGTTTGTG CAGTTCCAGC TGACTGTTAA TGTTGTCGCA 
CTTCTGGTTA ACTTCTCTTC AGCGTGCTTT ACGGGAGATG CTCCACTGAC AGCTGTTCAA 
CTTCTTTGGG TTAACATGAT CATGGATACC CTAGGAGCGC TTGCACTTGC AACTGAACCA 
CCTGATGATA ACTTAATGAA GAAGTCACCA GTTGGAAGGG CAGGGAAATT CATCACAAAC 
GTGATGTGGA GGAATATTGT GGGGCAGTCA ATATTCCAGT TTGTGGTCAT TTGGTATCTA 
CAAACTCAAG GGAAGTACTT GTTTGGGCTT GAAGGCTCTG AAGCTGACAC TGTTCTAAAT 
ACAATCATAT TCAACACTTT CGTTTTCTGC CAGGTGTTCA ATGAGATAAG CTCGAGGGAC 
ATGGAGGAGA TCAATGTTAT CAAGGGATTG CCACAGAACT CCATTTTCAT GTGCATCCTT 
GCTGGCACCA TCACCGTCCA GTTCATCCTT GTCCAGTTCC TGGGAGATTT TGCCAACACC 
GCACCTCTCA CCCAGCTCCA ATGGCTTGTC AGCATTCTCT TTGGCCTCCT CGGGATGCCC 
ATTGCTGCTG CCATCAAACT GATTCCTGTG GAACCTCATG AAGACAACAG GCGAATATCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002466818.2
CDS	281..5503
Translation	MSAAAGTPPEGGGDRPWQSYHTAYTNAKAGMEGVDKEKVQKVIYEMSKGSKYFENEQRKEAVTKQKIEHLRAQCAALTDNDISHFQKVAEKKMLELEASRDLSKIWLHTDMDAFYAAVETLENPSLKGKPLAVGSMSMIATASYEARKFGVRAAMPGFIGCKLCPGLVFVRPNFERYTHYSELTRKVFQRYDPNFIATSLDEAYLNITNVCIERGITGEEVATELRSAIYQETGLTCSAGVAPNRMIAKVCSDINKPNGQFILPNDREAVLTFVSTLPIRKIGGIGKVTEQMLRQVLGISTCQEMLQKAAFLCALFSEGSADFFLSVGLGLGGTETPEQRQRKSISCERTFAATNDSSLLFEKLDSLAENLTDDLQKEGLKGKTLTLKLKTADFEVRTRAVTTRNYINSKEDILVYATKLLKAEMPLSLRLMGLRMSQLHDEKDDPSTSTQKTLDIFFRSSNGNSNVNGNVQRITNTSGQDNDSISLTTKEYLVPDAGTGVSTDQQDFFLHDESCFIPEQRSFGNHSNEAVISKPLGGTKLDDVSSSAKVAPAEKLNEPDNLISPKAVASSSKPDQQSWIDGYICSICGFELPPGFEEERLEHSDFHLAETLQQEEAVDGTRHISNERLADRERPCSTTPTPKKKLKSSKEGKHIPIDAFFTKCNKNFIQCLYLSVRPPAARGQKNSASTGEREQERTRGGRGKTAIMHSGVDARPGPLLPLPGASPAPCSGRRVPWPRSGLGAALRRPKNLHGRLRFEPLPAGDLCKWAHREKLRVAVLVSKSTLQSEHGVSLQNGRVVPEGVKAAGFQISADDLASVVENRDAEKLTAHGQLDGIADKLATSLADGITTDECSLNQRQDMYGVNKFTESEVRSLWEFVWEALQDTTLVILLACALVSFVVGVATEGWPSGAHDGIGIFTSILLVVSVTATSNYQQSLQFRDLDKEKRKISIQVTRDGFRQRILIDDLLPGDVVHLAVGDQVPADGLFISGYSVLINESSLTGESEPVVINEDNPFLLSGTKVLDGSCKMLVTAVGMRTQWGKLMAAITESGDDETPLQGKLNGVANTIGNIGLFFALLTFVILSQGLVAQKYADGLLLSWSGEDVLEILEHFSIAVTIVVVAVPEGLPLAVTLSLAFAMKKMMNEKALVRQLAACETMGSATVICSDKTGTLTTNRMSVMKACICGNIMEVTNPPVLSSFSSKLPEFALQILLESIFNNTAGEVVINQDGNCQILGTPTEAALLDFALSIGGDFKEKRQETKIVKVEPFNSTKKRMSTILELPGGGYRAHCKGASEVVLAACDKFIDARGTIVALDKTATKKLSDIIETFSKEALRTLCLAYREMDDSFSIDEQIPLQGYTCIGIVGIKDPVRPGVRQSVATCRSAGIEVRMVTGDNINTAKAIARECGILTEDGIAIEGAEFREKNPKELLELIPKMQVLARSSPLDKHTLVKHLRTTFNEVVAVTGDGTNDAPALREADIGLAMGIAGTEVAKESADVVILDDNFSTIVTVAKWGRSVYVNIQKFVQFQLTVNVVALLVNFSSACFTGDAPLTAVQLLWVNMIMDTLGALALATEPPDDNLMKKSPVGRAGKFITNVMWRNIVGQSIFQFVVIWYLQTQGKYLFGLEGSEADTVLNTIIFNTFVFCQVFNEISSRDMEEINVIKGLPQNSIFMCILAGTITVQFILVQFLGDFANTAPLTQLQWLVSILFGLLGMPIAAAIKLIPVEPHEDNRRIS

Target ORF information:

RefSeq Version	XM_002466773.2
Organism	Sorghum bicolor(sorghum)
Definition	Sorghum bicolor calcium-transporting ATPase 3, plasma membrane-type (LOC8062510), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002466773.2

ORF Insert Sequence:

ATGTCGGCCG CGGCGGGGAC GCCACCGGAA GGCGGAGGGG ATCGGCCGTG GCAGTCTTAC 
CATACCGCAT ACACCAACGC CAAAGCTGGA ATGGAGGGTG TTGACAAAGA GAAGGTGCAG 
AAGGTAATCT ATGAAATGAG CAAGGGCTCA AAATATTTCG AAAATGAGCA GAGGAAGGAG 
GCAGTGACCA AACAAAAGAT TGAACATCTC CGTGCACAAT GTGCAGCATT GACTGATAAT 
GACATATCAC ATTTTCAGAA GGTTGCTGAG AAGAAAATGT TAGAGTTGGA AGCTTCACGA 
GATCTTTCAA AGATATGGCT TCACACTGAC ATGGATGCCT TTTATGCTGC TGTTGAGACA 
TTGGAGAATC CATCCTTGAA GGGAAAACCC TTGGCGGTTG GTAGCATGTC TATGATAGCT 
ACTGCCAGTT ATGAGGCACG TAAGTTTGGA GTGCGTGCAG CGATGCCTGG TTTTATTGGA 
TGCAAACTGT GTCCTGGCTT GGTTTTTGTC CGACCAAACT TTGAGAGATA TACTCACTAT 
AGCGAGCTGA CAAGAAAAGT TTTCCAGAGG TATGATCCCA ATTTCATTGC AACAAGTCTA 
GATGAAGCAT ACCTCAACAT AACAAATGTT TGCATTGAGA GAGGCATTAC AGGCGAGGAG 
GTTGCTACTG AACTTAGGTC TGCCATTTAC CAGGAAACTG GTCTAACATG TAGTGCTGGG 
GTTGCTCCAA ACCGTATGAT TGCAAAGGTT TGCTCTGATA TTAACAAGCC CAATGGGCAG 
TTTATTTTGC CAAATGACCG GGAAGCTGTA TTGACATTTG TATCTACATT GCCTATAAGA 
AAGATAGGTG GCATAGGTAA GGTCACAGAA CAGATGTTAC GTCAGGTTCT TGGAATCAGT 
ACATGCCAGG AGATGCTGCA AAAGGCTGCT TTTTTGTGTG CTCTTTTTTC GGAAGGCTCA 
GCTGATTTCT TTCTATCGGT TGGTCTTGGG TTAGGAGGTA CAGAAACCCC GGAGCAAAGG 
CAAAGGAAAA GTATCAGTTG CGAGCGAACT TTCGCAGCAA CAAATGATTC TTCTTTGCTT 
TTCGAAAAAT TAGATAGCCT TGCAGAAAAT TTGACTGATG ATCTGCAAAA GGAGGGTTTG 
AAGGGAAAGA CACTAACACT CAAACTGAAG ACTGCAGATT TTGAGGTTCG TACAAGGGCA 
GTAACTACAC GGAACTACAT CAACTCCAAG GAAGATATCC TGGTCTATGC TACAAAGTTG 
CTTAAGGCTG AAATGCCTCT TTCTTTGAGA CTGATGGGGT TGCGGATGTC TCAGCTACAT 
GATGAGAAGG ATGATCCATC TACTTCAACA CAAAAGACAC TAGATATATT TTTTCGTTCA 
TCAAACGGTA ACTCAAATGT CAATGGAAAT GTTCAGAGAA TCACTAATAC CTCTGGGCAG 
GATAATGATT CTATTAGTCT CACAACAAAG GAATACTTGG TACCTGATGC TGGAACAGGT 
GTTTCCACAG ATCAACAGGA TTTCTTCCTA CATGATGAGA GCTGTTTTAT TCCCGAACAA 
AGGAGTTTTG GCAATCATAG TAATGAGGCT GTCATAAGCA AGCCGTTGGG TGGAACAAAG 
TTAGATGATG TGTCTTCCAG TGCAAAGGTT GCGCCTGCTG AGAAGTTGAA TGAACCTGAT 
AACTTGATCA GTCCGAAGGC TGTGGCTTCA TCAAGCAAAC CTGATCAGCA ATCATGGATT 
GATGGCTACA TTTGTTCCAT TTGTGGATTT GAACTACCTC CAGGCTTTGA GGAGGAGAGA 
CTAGAACATT CAGATTTTCA CTTGGCTGAA ACACTCCAGC AGGAAGAAGC AGTGGACGGT 
ACAAGACACA TCTCAAATGA GAGGCTGGCT GACAGAGAGA GACCATGCTC AACTACACCC 
ACACCCAAGA AGAAACTGAA GTCTTCTAAA GAAGGAAAAC ATATTCCGAT TGACGCTTTC 
TTCACAAAGT GTAATAAGAA CTTTATCCAG TGCTTGTACC TGTCAGTCCG CCCGCCAGCT 
GCACGTGGTC AGAAAAATTC AGCAAGCACT GGAGAGCGAG AGCAAGAGCG AACTAGGGGA 
GGAAGGGGGA AGACGGCAAT CATGCACAGC GGCGTCGACG CTCGGCCGGG ACCCCTCCTC 
CCCCTCCCAG GCGCATCACC AGCTCCCTGC TCCGGCCGCC GCGTCCCATG GCCGCGGTCG 
GGGTTAGGGG CTGCGCTGCG GCGGCCAAAG AACCTCCACG GCCGCCTCCG CTTCGAGCCC 
CTCCCCGCCG GCGACCTCTG CAAGTGGGCC CACCGTGAGA AGCTACGTGT TGCTGTGCTT 
GTATCGAAGT CCACGCTGCA GAGCGAACAT GGTGTTTCAC TTCAGAATGG GCGTGTTGTC 
CCAGAAGGTG TCAAAGCTGC TGGGTTTCAG ATCTCTGCCG ATGACCTAGC ATCAGTTGTT 
GAAAATCGTG ACGCTGAAAA ATTGACTGCG CATGGCCAGC TGGATGGGAT CGCAGACAAG 
TTGGCGACGT CATTAGCTGA TGGAATCACC ACAGATGAGT GCAGCCTGAA TCAACGGCAG 
GACATGTATG GAGTGAACAA GTTCACGGAG AGTGAGGTCC GCAGCTTATG GGAGTTTGTG 
TGGGAGGCAT TGCAAGATAC TACTCTTGTA ATTCTTCTAG CATGTGCCCT TGTATCTTTT 
GTCGTTGGCG TTGCCACGGA AGGATGGCCA AGTGGTGCCC ATGATGGCAT TGGAATTTTT 
ACAAGTATCC TCTTGGTTGT TTCTGTTACC GCGACGAGCA ATTATCAGCA GTCTTTGCAA 
TTCAGGGATC TGGACAAAGA GAAAAGGAAA ATTTCTATTC AGGTTACCAG AGATGGGTTT 
AGACAAAGGA TATTGATAGA TGATCTTCTT CCTGGTGATG TTGTCCACCT AGCTGTTGGA 
GATCAGGTTC CTGCAGATGG CCTCTTTATT TCAGGGTATT CTGTATTGAT CAACGAGTCC 
AGCCTAACTG GTGAGAGTGA ACCTGTTGTC ATAAATGAAG ATAACCCTTT TCTTTTGTCA 
GGGACTAAAG TCCTAGATGG GTCATGCAAA ATGCTGGTTA CAGCAGTAGG GATGCGAACA 
CAGTGGGGTA AACTAATGGC TGCCATCACT GAAAGTGGGG ATGATGAAAC TCCACTGCAG 
GGTAAACTAA ATGGAGTTGC AAATACTATT GGAAATATCG GTCTGTTTTT TGCCCTTTTA 
ACTTTTGTTA TCCTCTCGCA AGGGTTAGTG GCCCAGAAAT ACGCTGATGG GCTTCTTTTA 
AGCTGGTCTG GAGAAGATGT ACTGGAGATA CTGGAACATT TTTCCATTGC AGTCACAATT 
GTTGTTGTTG CGGTTCCTGA GGGATTGCCT TTAGCAGTGA CATTGAGCCT TGCATTTGCA 
ATGAAGAAGA TGATGAATGA GAAGGCACTG GTACGACAGT TAGCTGCCTG TGAAACTATG 
GGCTCAGCCA CTGTCATCTG CAGTGATAAG ACAGGAACTC TTACAACTAA TCGCATGTCT 
GTCATGAAGG CCTGCATATG TGGAAACATC ATGGAAGTGA CCAATCCACC AGTGCTTTCA 
AGTTTTTCTT CCAAACTCCC AGAATTTGCT TTGCAAATTC TTCTGGAATC CATTTTTAAC 
AACACTGCTG GTGAAGTGGT GATTAACCAA GATGGGAACT GTCAGATACT AGGGACCCCA 
ACTGAGGCTG CTTTGTTGGA TTTTGCATTG TCAATAGGTG GTGACTTCAA AGAAAAACGG 
CAGGAAACTA AGATTGTTAA AGTCGAGCCT TTTAATTCAA CAAAAAAGAG GATGAGCACC 
ATTCTCGAGC TTCCTGGTGG AGGTTACCGT GCACATTGTA AAGGTGCTTC AGAAGTAGTC 
TTGGCTGCCT GTGATAAATT CATAGATGCC AGAGGTACTA TTGTTGCACT TGATAAAACA 
GCTACAAAGA AACTCAGTGA CATCATCGAA ACCTTTTCTA AAGAAGCACT CAGGACACTC 
TGCCTTGCTT ATCGGGAAAT GGATGATAGC TTTTCCATTG ATGAGCAAAT ACCGCTACAA 
GGATACACAT GCATTGGTAT TGTTGGGATC AAAGATCCTG TTCGTCCAGG TGTCAGGCAG 
TCTGTGGCAA CTTGCCGGTC TGCTGGCATT GAGGTTCGAA TGGTTACAGG AGACAACATA 
AATACAGCAA AGGCAATTGC TCGTGAATGC GGTATACTTA CTGAGGATGG TATTGCCATT 
GAAGGTGCTG AGTTCAGGGA GAAAAATCCC AAAGAACTCC TTGAGTTGAT TCCAAAGATG 
CAGGTCCTGG CCCGATCATC ACCACTTGAT AAGCATACAC TTGTGAAACA CCTGCGTACC 
ACATTTAATG AGGTTGTTGC TGTGACTGGT GATGGTACAA ATGACGCACC GGCGCTACGT 
GAGGCAGATA TTGGACTTGC CATGGGAATT GCAGGGACTG AGGTAGCAAA AGAGAGCGCT 
GATGTTGTTA TTCTTGATGA TAATTTCTCC ACTATCGTAA CAGTTGCCAA ATGGGGACGC 
TCCGTTTATG TCAACATTCA AAAGTTTGTG CAGTTCCAGC TGACTGTTAA TGTTGTCGCA 
CTTCTGGTTA ACTTCTCTTC AGCGTGCTTT ACGGGAGATG CTCCACTGAC AGCTGTTCAA 
CTTCTTTGGG TTAACATGAT CATGGATACC CTAGGAGCGC TTGCACTTGC AACTGAACCA 
CCTGATGATA ACTTAATGAA GAAGTCACCA GTTGGAAGGG CAGGGAAATT CATCACAAAC 
GTGATGTGGA GGAATATTGT GGGGCAGTCA ATATTCCAGT TTGTGGTCAT TTGGTATCTA 
CAAACTCAAG GGAAGTACTT GTTTGGGCTT GAAGGCTCTG AAGCTGACAC TGTTCTAAAT 
ACAATCATAT TCAACACTTT CGTTTTCTGC CAGGTGTTCA ATGAGATAAG CTCGAGGGAC 
ATGGAGGAGA TCAATGTTAT CAAGGGATTG CCACAGAACT CCATTTTCAT GTGCATCCTT 
GCTGGCACCA TCACCGTCCA GTTCATCCTT GTCCAGTTCC TGGGAGATTT TGCCAACACC 
GCACCTCTCA CCCAGCTCCA ATGGCTTGTC AGCATTCTCT TTGGCCTCCT CGGGATGCCC 
ATTGCTGCTG CCATCAAACT GATTCCTGTG GAACCTCATG AAGACAACAG GCGAATATCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Sorghum bicolor genome and the diversification of grasses.
Nature457(7229)551-6(2009 Jan)
Paterson AH,Bowers JE,Bruggmann R,Dubchak I,Grimwood J,Gundlach H,Haberer G,Hellsten U,Mitros T,Poliakov A,Schmutz J,Spannagl M,Tang H,Wang X,Wicker T,Bharti AK,Chapman J,Feltus FA,Gowik U,Grigoriev IV,Lyons E,Maher CA,Martis M,Narechania A,Otillar RP,Penning BW,Salamov AA,Wang Y,Zhang L,Carpita NC,Freeling M,Gingle AR,Hash CT,Keller B,Klein P,Kresovich S,McCann MC,Ming R,Peterson DG,Mehboob-ur-Rahman,Ware D,Westhoff P,Mayer KF,Messing J,Rokhsar DS

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LOC8062510 cDNA ORF clone, Sorghum bicolor(sorghum)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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