Unc13b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Unc13b
Entrez Gene ID	64830
Full Name	unc-13 homolog B
Synonyms	Munc13-2,Unc13h2
General protein information	Preferred Names unc-13 homolog B Names protein unc-13 homolog B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22
Summary	brain specific protein that may have a role in neurotransmitter release [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042579.1	NP_001036044.1	protein unc-13 homolog B isoform u
NM_022862.1	NP_074053.1	protein unc-13 homolog B isoform b

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Unc13b	NP_067443.2
Canis lupus familiaris (dog)	UNC13B	XP_005626825.1
Gallus gallus (chicken)	LOC100858799	XP_004937184.1
Pan troglodytes (chimpanzee)	UNC13B	XP_519737.2
Macaca mulatta (Rhesus monkey)	UNC13B	XP_001085980.2
Danio rerio (zebrafish)	LOC100329499	XP_002663560.3
Homo sapiens (human)	UNC13B	NP_006368.3
Bos taurus (cattle)	UNC13B	NP_001180168.1
Rattus norvegicus (Norway rat)	Unc13b	NP_001036044.1

Gene Ontology
Bibliography

Related articles in PubMed

ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.
Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Südhof TC, Rhee JS, Brose N
The Journal of cell biology216(4)1143-1161(2017 04)

Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis.
Man KN, Imig C, Walter AM, Pinheiro PS, Stevens DR, Rettig J, Sørensen JB, Cooper BH, Brose N, Wojcik SM
eLife4(2015 Nov)

The morphological and molecular nature of synaptic vesicle priming at presynaptic active zones.
Imig C, Min SW, Krinner S, Arancillo M, Rosenmund C, Südhof TC, Rhee J, Brose N, Cooper BH
Neuron84(2)416-31(2014 Oct)

CAPS and Munc13 utilize distinct PIP2-linked mechanisms to promote vesicle exocytosis.
Kabachinski G, Yamaga M, Kielar-Grevstad DM, Bruinsma S, Martin TF
Molecular biology of the cell25(4)508-21(2014 Feb)

The Munc13 proteins differentially regulate readily releasable pool dynamics and calcium-dependent recovery at a central synapse.
Chen Z, Cooper B, Kalla S, Varoqueaux F, Young SM Jr
The Journal of neuroscience : the official journal of the Society for Neuroscience33(19)8336-51(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Unc13b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Unc13b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06391	NM_022862.1 Latest version!	Rattus norvegicus unc-13 homolog B (Unc13b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa09925	NM_001042579.1 Latest version!	Rattus norvegicus unc-13 homolog B (Unc13b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Unc13b ( NM_022862.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_074053.1 Rattus norvegicus unc-13 homolog B (Unc13b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa06391
Clone ID Related Accession (Same CDS sequence)	NM_022862.1
Accession Version	NM_022862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568217600000
Organism	Rattus norvegicus(Norway rat)
Product	protein unc-13 homolog B isoform b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U24071.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U24071.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAACGAC TGTTGCGGGA GTCTGAGGAA GAAATCATGC TGACACTGGG ACCTTCATCC 
AGCCTCTCAC CAGATCAGGT CAGGACAGAG ACTGTGTGTA TTGTCAAGGG CAAGTCCACA 
GGTCCTACTG GGTCCTTGCC AGAGGACAAC TTCCCACCCC CTTGCTGTGA GTCAGCAGAC 
TCCACCACTA GTGGAGAGAG AGATAGGAAC CTGGCCCAAC TTGGTAGCTT TGAGCAGCAA 
GCCAGTAGCC AACCTTCTCT GGCCTGCACT GCTTGTGCTT CAGGGTCTGA TTCTCGGGAA 
CTCTCGCCTG CCAGCATAAC AAGCTGTTCA GAGCCTTCAG AGAGAAACAA AGCCAGGCCC 
ATATTCCCCA GAGGTCCTGG CCAAAGATGT AGGCATGAGC ATCAGGAGCC TCTGGGAGAT 
GTAGTAGAAT ATATCATCAG AGAGCTGCAA GGAATCAGTC GTCTCCAGTC TGAGATTGCT 
GAGCTGCAAC AGCACTTGAA CCAAGTCCGG GGCTCTGTGG ATGAAGTATC TAGCTGTGTA 
GATTCAGTGC TGAGTGAAAT AGAAGGCTTG CACGTGGGTA GCAGCTCCCT GGGCAAAGTA 
CGCCATGGGG AAAAGGCCCA GGAACTCCAT GTAGAAAGAT CCAGAGAGGA AGCTATTCTT 
TATTTGTATG GACTTCCTGA ACATGATGGG GAAAGCACTG TGGAACTGGT AGATAACTTT 
CTAGCTAAGC ACCTCTGTGT TAATGGCATG CAGTGCAACA GGTATGTCAG AGAGGCATAC 
AGGGCAGGCA CAGCTCCTGC CCCCCGGCCC ACTGTGGTGA AACTTGTCCA CCCAGAGCAC 
AGAGACCTCA TCTTGCAAAA ATCCATCCTC TTGCAGAGTG TGGGGGTCAG GGTTGCTACC 
AGAGAGGAAC CAGTCTGGCC AGAAGGGTGT AAGAATCCCC CAAAGGAGTC TCTCTCATGC 
TTGCAACAAT TTCAAGACCA CAGTCGGAAC CATCAGGGTA AACCAGCTCT TCAGCTAGAA 
ACAGGGAACA GAAGACAAAT GTCGGGACCA CACCAGATGA GGACTCAGAA TCAACATAGA 
GAACTCCAGG CTTCTGAGCA TCAAGGGCTT AGTTTCCTAC CAAAGGATGG CTCAGCAAAA 
CAGAGTGATG TGTCTAAGCT GCAGGATGAA GTAAAAGGAA CATCAGGAGC TCCCCAGGTA 
ATCAGTGATC CTTGTGGTGA ACTTTCTCTC CTCCATCAAC TTGAGGGGTC TTCCCCAGTG 
TTAATCCCAA AAGAGGAAGA TTGTGGAAAA TTACAGATTT TCAAACAGGA CAGCCAAGAG 
CATAAGGCAT GTAACGTGAC AAAGCTACAA AGTGATTGCA ACAATGCAAT TAAAGCCAGC 
AGTTGCCTTT CATTGTCTGG ACCACTGAAA GCAGAGAAGG TAAACGCAGA GGACAGGATG 
CTAGGCGGTG AAGATGGACT TGATATTCTG AGCCCAAAGC AGCTGGAGGA CCTTTTGGCA 
GACAAGTCTA GGAGGTTTGC GACTCTGAAT CCTGACAGCG CAGTGGAGGA AGTGATCATA 
GGACCCGAGA CTTTCAGTAA CATGGTGCAT ATTGACCTGA ATGAAGAAGA GACCTGTACT 
GCCCAGGTCC TTAAGAATGT CTTTGATAAG TCCTCATGTG TTCTGGGTGG CTCCCAGGAG 
GATGAAGATG TAGAAATTAA ATTTCACACA ACTAAGCTGA GTCGAGCGAT CCACCACTTC 
CGTTTGGCTC TTCAGGGGGT ATTTCAGAAG CTGGAGAACA ATGGGTCCAT CAGTCCTGAG 
GACCTGGAAA GCAATGAGAG TGGTTCCCAG TCAGAAAACA GTGATAGACT TCTTTGGACA 
GTAAGTTCAG GAGGTGCCCA CGATTGTTCA GTGGAGAGTC CAGCAAGTCA GGGGAGTGAG 
AGCTTGCTCA GTGTGGTCTC TGGTGGAGTA GGCATCTCTG TACAGGGTGA TCAGACACCT 
CAAGCACCAA GTAACTTCTC TCTTGCTTCT AACAACTCAC CTCTTACAAA CTCATTGCTC 
AGTTTCCCTC TAGCCCCAGG TCTGGGAAAC GAGACTTGTT CCAGGCCTGA CTCTCCTAAT 
CAAGGCAAAC TGAGTTTAGA ACAAGTATGT GCAGAGACCA TTTACCTTAA CAAATGCATC 
AACAATTTTA AAAACGTCCT AAGAGAGAAA AGACTGAGGC AGAAAAAACT TTTGCAAGAG 
CTAGTACAGA CGGCAAGTCA TCTTTCAGTA GAAGACATCC CCTCAGAAGG AAAGCGAGAA 
GCTCTTCAGA TTTCAGATGA TGGTGACCCC TCCTTGCCTC AGTGGCTCCC AGAAGGGCCA 
GCGGGAGGGC TCTATGGCAT CGACAGCATG CCAGATCTAC GAAGAAAGAA GCCACTGCCA 
CTTGTCAGTG ATCTGGCTAT GTCACTGGTC CAGTCACGGA AAGCAGGGAT TACTTCCGCT 
ATGGCTACAC GTACCTCTCT CAAGGATGAA GATCTGAAAT CCCATGTGTA TAAGAAAACT 
CTGCAGGCCT TAATCTACCC CATCTCGTGC ACCACGCCCC ACAACTTTGA GGTCTGGTCT 
GCCACGACAC CCACCTACTG CTATGAGTGT GAAGGCTTGC TCTGGGGCCT TGCCCGGCAA 
GGCATGCGCT GCAGCGAGTG TGGCGTCAAG TGCCATGAGA AGTGCCAAGA CCTGCTCAAT 
GCCGATTGCC TACAGCGGGC CGCTGAAAAG AGCTCTAAGC ACGGAGCTGA GGACCGGACT 
CAGAACATTA TCATGGCCAT GAAGGACCGT ATGAAGATCC GAGAACGGAA TAAACCAGAG 
ATCTTTGAGG TTATCCGGGA TGTGTTCACA GTGAGCAAAG TTGCCCACGT GCAGCAGATG 
AAAACAGTAA AGCAGAGTGT CCTGGATGGC ACCTCCAAGT GGTCGGCCAA AATTACCATC 
ACGGTGGTTT GTGCCCAGGG CCTACAAGCC AAAGACAAGA CAGGATCCAG TGACCCTTAT 
GTGACTGTGC AAGTTGGGAA AACCAAGAAG CGCACTAAGA CCATTTTTGG AAATTTGAAT 
CCTGTTTGGG AGGAGAAGTT CCATTTTGAG TGCCACAACT CTTCCGATCG AATTAAGGTA 
CGTGTGTGGG ATGAGGATGA TGACATCAAA TCCAGAGTAA AGCAGCGGCT GAAGCGAGAG 
TCGGATGATT TCCTTGGCCA AACTATCATT GAAGTTCGAA CGCTGAGTGG AGAGATGGAT 
GTCTGGTATA ATCTGGAGAA GAGAACAGAC AAGTCAGCTG TCTCAGGGGC TATCCGACTG 
CAAATCAATG TGGAGATCAA GGGGGAGGAG AAGGTAGCCC CATACCATGT GCAATACACA 
TGTCTCCATG AGAATCTTTT CCATTACCTC ACAGACATTC AGGGCAGTGG AGGAGTCTGG 
ATCCCAGACG CTCGGGGGGA CGATGCATGG AAGGTGTACT TTGACGAGAC AGCCCAAGAA 
ATTGTGGATG AATTTGCCAT GCGCTATGGC ATCGAGTCCA TATATCAGGC CATGACGCAC 
TTTGCGTGTT TGTCATCCAA GTACATGTGT CCTGGCGTAC CAGCCGTGAT GAGTACCTTA 
CTGGCCAACA TCAATGCGTA TTATGCTCAC ACAACCGCCT CCACTAACGT CTCTGCATCT 
GACCGCTTTG CCGCCTCCAA CTTTGGTAAA GAAAGATTTG TAAAACTGCT GGACCAGCTA 
CACAACTCAC TGAGGATAGA CCTGTCTACA TACAGGAATA ACTTTCCTGC TGGGAGCCCT 
GAGCGGCTCC AGGATTTAAA ATCTACAGTG GACTTGCTGA CCAGCATTAC TTTCTTCAGG 
ATGAAGGTGC AAGAACTTCA GAGCCCCCCA AGAGCCAGCC AGGTGGTAAA GGACTGTGTG 
AAGGCCTGCT TGAACTCTAC ATATGAATAC ATCTTCAACA ACTGCCATGA CCTCTACAGT 
CACCAATACC AGCTGCAGGA ACAACCACTA GAGGAACCAG GGCCCAGCAT TCGGAACTTG 
GATTTCTGGC CCAAACTTAT CACACTGATT GTATCAATCA TAGAGGAGGA TAAGAATTCC 
TACACACCTG TTCTGAACCA GTTTCCTCAG GAGTTGAATG TAGGAAAAGT CAGTGCAGAA 
GTGATGTGGC ACCTGTTTGC TCAAGACATG AAATATGCAC TGGAGGAACA TGAGAAAGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATATGTGC GGGAGCTGCC TGCCCTCCAG GGGCAGGTGC CTGAGTACCC AGCGTGGTTT 
GAGCAGTTCG TGCTACAGTG GCTAGATGAA AATGAAGACG TGTCCCTGGA ATTCCTTCGT 
GGGGGCTTGG AACGAGATAA GAGAGATGGG TTCCAGCAAA CATCGGAGCA TGCCTTGTTT 
TCCTGTTCTG TGGTGGACGT CTTTACACAA CTCAACCAGA GCTTTGAGAT CATCCGGAAA 
TTGGAATGTC CAGACCCCAG CATCCTTGCC CATTATATGA GAAGGTTTGC TAAGACCATC 
GGCAAAGTGC TGATACAGTA TGCAGACATC TTATCAAAGA ACTTCCCAGC TTACTGCACA 
AAGGAGAGAC TGCCCTGTAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAAGAG CTGGACTCTG AAGCTGCAGA CAGTCTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTGTT TGGAAACAGC 
TTCCAGGTGC GGATCGACGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAAGTACGG 
GGCACAGGGA ATGCATCCCC TAACGCCAGA GCCTCGGTGG CTCAGGATGC AGATAGTGTG 
CTGCGACCTC TCATGGATTT CCTGGATGGC AACCTCACAC TGTTTGCCAC TGTGTGTGAG 
AAGACAGTGC TGAAGCGAGT CCTGAAGGAG CTCTGGCGAG TGGTGATGAA CACCATGGAG 
CGGGTCATCG TCCTGCCCCC ACTCATTGAC CAGACAGGCA CCCAGCTGAT CTTAACTGCT 
GCCAAGGAGC TGAGCCAGCT TTCCAAACTC AAGGACCACA TGGTACGAGA GGAAACACGG 
AATCTCACTC CAAAGCAGTG TGCTGTCCTC GACTTGGCCT TGGACACCGT CAAGCAATAC 
TTCCATGCAG GAGGCAATGG TCTGAAGAAA ACCTTCCTGG AAAAGAGCCC AGACCTGCAG 
TCTCTACGTT ATGCCCTCTC TCTGTATACA CAGACCACAG ACACTCTCAT CAAGACATTT 
GTGCGCTCAC AGACTGCCCA AGTACATGAT GGGAAAGGTA TTAGGTTTAC TGCTAACGAG 
GACATTCGGC CAGAAAAGGG GGCTGGTGTG GACGACCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGCACTGGG GAGCATAAGG TCACAGTGAA AGTGGTAGCA 
GCCAATGATC TCAAGTGGCA GACAGCAGGT ATGTTCCGGC CTTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGCGA TAAGAAGAGG AAGTTCACAA CCAAGTCTAA AAGTAACAGC 
TGGACTCCCA AGTACAACGA GACTTTTCAT TTCCTCCTGG GAAATGAAGA GGGTCCCGAG 
GCCTATGAAT TGCAGATATG CGTGAAGGAT TACTGCTTTG CCCGGGAGGA TCGTGTGATA 
GGACTGGCAG TGATGCCCCT GAGGGATGTG GCAGCTAAGG GCAGCTGTGC CTGCTGGTGC 
CCGTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CCATTCTCCG GATTCTGTCT 
CAGAGGAGCA ATGATGAGGT AGCCCGAGAG TTCGTGAAGC TCAAGTCAGA GTCTCGATCC 
ATAGAGGAAG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_074053.1
CDS	486..6443
Translation	MKRLLRESEEEIMLTLGPSSSLSPDQVRTETVCIVKGKSTGPTGSLPEDNFPPPCCESADSTTSGERDRNLAQLGSFEQQASSQPSLACTACASGSDSRELSPASITSCSEPSERNKARPIFPRGPGQRCRHEHQEPLGDVVEYIIRELQGISRLQSEIAELQQHLNQVRGSVDEVSSCVDSVLSEIEGLHVGSSSLGKVRHGEKAQELHVERSREEAILYLYGLPEHDGESTVELVDNFLAKHLCVNGMQCNRYVREAYRAGTAPAPRPTVVKLVHPEHRDLILQKSILLQSVGVRVATREEPVWPEGCKNPPKESLSCLQQFQDHSRNHQGKPALQLETGNRRQMSGPHQMRTQNQHRELQASEHQGLSFLPKDGSAKQSDVSKLQDEVKGTSGAPQVISDPCGELSLLHQLEGSSPVLIPKEEDCGKLQIFKQDSQEHKACNVTKLQSDCNNAIKASSCLSLSGPLKAEKVNAEDRMLGGEDGLDILSPKQLEDLLADKSRRFATLNPDSAVEEVIIGPETFSNMVHIDLNEEETCTAQVLKNVFDKSSCVLGGSQEDEDVEIKFHTTKLSRAIHHFRLALQGVFQKLENNGSISPEDLESNESGSQSENSDRLLWTVSSGGAHDCSVESPASQGSESLLSVVSGGVGISVQGDQTPQAPSNFSLASNNSPLTNSLLSFPLAPGLGNETCSRPDSPNQGKLSLEQVCAETIYLNKCINNFKNVLREKRLRQKKLLQELVQTASHLSVEDIPSEGKREALQISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEDLKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQINVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPDARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRELPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGGLERDKRDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLIQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLIDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTVKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNSWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSIEEGS

Target ORF information:

RefSeq Version	NM_022862.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus unc-13 homolog B (Unc13b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022862.1

ORF Insert Sequence:

ATGAAACGAC TGTTGCGGGA GTCTGAGGAA GAAATCATGC TGACACTGGG ACCTTCATCC 
AGCCTCTCAC CAGATCAGGT CAGGACAGAG ACTGTGTGTA TTGTCAAGGG CAAGTCCACA 
GGTCCTACTG GGTCCTTGCC AGAGGACAAC TTCCCACCCC CTTGCTGTGA GTCAGCAGAC 
TCCACCACTA GTGGAGAGAG AGATAGGAAC CTGGCCCAAC TTGGTAGCTT TGAGCAGCAA 
GCCAGTAGCC AACCTTCTCT GGCCTGCACT GCTTGTGCTT CAGGGTCTGA TTCTCGGGAA 
CTCTCGCCTG CCAGCATAAC AAGCTGTTCA GAGCCTTCAG AGAGAAACAA AGCCAGGCCC 
ATATTCCCCA GAGGTCCTGG CCAAAGATGT AGGCATGAGC ATCAGGAGCC TCTGGGAGAT 
GTAGTAGAAT ATATCATCAG AGAGCTGCAA GGAATCAGTC GTCTCCAGTC TGAGATTGCT 
GAGCTGCAAC AGCACTTGAA CCAAGTCCGG GGCTCTGTGG ATGAAGTATC TAGCTGTGTA 
GATTCAGTGC TGAGTGAAAT AGAAGGCTTG CACGTGGGTA GCAGCTCCCT GGGCAAAGTA 
CGCCATGGGG AAAAGGCCCA GGAACTCCAT GTAGAAAGAT CCAGAGAGGA AGCTATTCTT 
TATTTGTATG GACTTCCTGA ACATGATGGG GAAAGCACTG TGGAACTGGT AGATAACTTT 
CTAGCTAAGC ACCTCTGTGT TAATGGCATG CAGTGCAACA GGTATGTCAG AGAGGCATAC 
AGGGCAGGCA CAGCTCCTGC CCCCCGGCCC ACTGTGGTGA AACTTGTCCA CCCAGAGCAC 
AGAGACCTCA TCTTGCAAAA ATCCATCCTC TTGCAGAGTG TGGGGGTCAG GGTTGCTACC 
AGAGAGGAAC CAGTCTGGCC AGAAGGGTGT AAGAATCCCC CAAAGGAGTC TCTCTCATGC 
TTGCAACAAT TTCAAGACCA CAGTCGGAAC CATCAGGGTA AACCAGCTCT TCAGCTAGAA 
ACAGGGAACA GAAGACAAAT GTCGGGACCA CACCAGATGA GGACTCAGAA TCAACATAGA 
GAACTCCAGG CTTCTGAGCA TCAAGGGCTT AGTTTCCTAC CAAAGGATGG CTCAGCAAAA 
CAGAGTGATG TGTCTAAGCT GCAGGATGAA GTAAAAGGAA CATCAGGAGC TCCCCAGGTA 
ATCAGTGATC CTTGTGGTGA ACTTTCTCTC CTCCATCAAC TTGAGGGGTC TTCCCCAGTG 
TTAATCCCAA AAGAGGAAGA TTGTGGAAAA TTACAGATTT TCAAACAGGA CAGCCAAGAG 
CATAAGGCAT GTAACGTGAC AAAGCTACAA AGTGATTGCA ACAATGCAAT TAAAGCCAGC 
AGTTGCCTTT CATTGTCTGG ACCACTGAAA GCAGAGAAGG TAAACGCAGA GGACAGGATG 
CTAGGCGGTG AAGATGGACT TGATATTCTG AGCCCAAAGC AGCTGGAGGA CCTTTTGGCA 
GACAAGTCTA GGAGGTTTGC GACTCTGAAT CCTGACAGCG CAGTGGAGGA AGTGATCATA 
GGACCCGAGA CTTTCAGTAA CATGGTGCAT ATTGACCTGA ATGAAGAAGA GACCTGTACT 
GCCCAGGTCC TTAAGAATGT CTTTGATAAG TCCTCATGTG TTCTGGGTGG CTCCCAGGAG 
GATGAAGATG TAGAAATTAA ATTTCACACA ACTAAGCTGA GTCGAGCGAT CCACCACTTC 
CGTTTGGCTC TTCAGGGGGT ATTTCAGAAG CTGGAGAACA ATGGGTCCAT CAGTCCTGAG 
GACCTGGAAA GCAATGAGAG TGGTTCCCAG TCAGAAAACA GTGATAGACT TCTTTGGACA 
GTAAGTTCAG GAGGTGCCCA CGATTGTTCA GTGGAGAGTC CAGCAAGTCA GGGGAGTGAG 
AGCTTGCTCA GTGTGGTCTC TGGTGGAGTA GGCATCTCTG TACAGGGTGA TCAGACACCT 
CAAGCACCAA GTAACTTCTC TCTTGCTTCT AACAACTCAC CTCTTACAAA CTCATTGCTC 
AGTTTCCCTC TAGCCCCAGG TCTGGGAAAC GAGACTTGTT CCAGGCCTGA CTCTCCTAAT 
CAAGGCAAAC TGAGTTTAGA ACAAGTATGT GCAGAGACCA TTTACCTTAA CAAATGCATC 
AACAATTTTA AAAACGTCCT AAGAGAGAAA AGACTGAGGC AGAAAAAACT TTTGCAAGAG 
CTAGTACAGA CGGCAAGTCA TCTTTCAGTA GAAGACATCC CCTCAGAAGG AAAGCGAGAA 
GCTCTTCAGA TTTCAGATGA TGGTGACCCC TCCTTGCCTC AGTGGCTCCC AGAAGGGCCA 
GCGGGAGGGC TCTATGGCAT CGACAGCATG CCAGATCTAC GAAGAAAGAA GCCACTGCCA 
CTTGTCAGTG ATCTGGCTAT GTCACTGGTC CAGTCACGGA AAGCAGGGAT TACTTCCGCT 
ATGGCTACAC GTACCTCTCT CAAGGATGAA GATCTGAAAT CCCATGTGTA TAAGAAAACT 
CTGCAGGCCT TAATCTACCC CATCTCGTGC ACCACGCCCC ACAACTTTGA GGTCTGGTCT 
GCCACGACAC CCACCTACTG CTATGAGTGT GAAGGCTTGC TCTGGGGCCT TGCCCGGCAA 
GGCATGCGCT GCAGCGAGTG TGGCGTCAAG TGCCATGAGA AGTGCCAAGA CCTGCTCAAT 
GCCGATTGCC TACAGCGGGC CGCTGAAAAG AGCTCTAAGC ACGGAGCTGA GGACCGGACT 
CAGAACATTA TCATGGCCAT GAAGGACCGT ATGAAGATCC GAGAACGGAA TAAACCAGAG 
ATCTTTGAGG TTATCCGGGA TGTGTTCACA GTGAGCAAAG TTGCCCACGT GCAGCAGATG 
AAAACAGTAA AGCAGAGTGT CCTGGATGGC ACCTCCAAGT GGTCGGCCAA AATTACCATC 
ACGGTGGTTT GTGCCCAGGG CCTACAAGCC AAAGACAAGA CAGGATCCAG TGACCCTTAT 
GTGACTGTGC AAGTTGGGAA AACCAAGAAG CGCACTAAGA CCATTTTTGG AAATTTGAAT 
CCTGTTTGGG AGGAGAAGTT CCATTTTGAG TGCCACAACT CTTCCGATCG AATTAAGGTA 
CGTGTGTGGG ATGAGGATGA TGACATCAAA TCCAGAGTAA AGCAGCGGCT GAAGCGAGAG 
TCGGATGATT TCCTTGGCCA AACTATCATT GAAGTTCGAA CGCTGAGTGG AGAGATGGAT 
GTCTGGTATA ATCTGGAGAA GAGAACAGAC AAGTCAGCTG TCTCAGGGGC TATCCGACTG 
CAAATCAATG TGGAGATCAA GGGGGAGGAG AAGGTAGCCC CATACCATGT GCAATACACA 
TGTCTCCATG AGAATCTTTT CCATTACCTC ACAGACATTC AGGGCAGTGG AGGAGTCTGG 
ATCCCAGACG CTCGGGGGGA CGATGCATGG AAGGTGTACT TTGACGAGAC AGCCCAAGAA 
ATTGTGGATG AATTTGCCAT GCGCTATGGC ATCGAGTCCA TATATCAGGC CATGACGCAC 
TTTGCGTGTT TGTCATCCAA GTACATGTGT CCTGGCGTAC CAGCCGTGAT GAGTACCTTA 
CTGGCCAACA TCAATGCGTA TTATGCTCAC ACAACCGCCT CCACTAACGT CTCTGCATCT 
GACCGCTTTG CCGCCTCCAA CTTTGGTAAA GAAAGATTTG TAAAACTGCT GGACCAGCTA 
CACAACTCAC TGAGGATAGA CCTGTCTACA TACAGGAATA ACTTTCCTGC TGGGAGCCCT 
GAGCGGCTCC AGGATTTAAA ATCTACAGTG GACTTGCTGA CCAGCATTAC TTTCTTCAGG 
ATGAAGGTGC AAGAACTTCA GAGCCCCCCA AGAGCCAGCC AGGTGGTAAA GGACTGTGTG 
AAGGCCTGCT TGAACTCTAC ATATGAATAC ATCTTCAACA ACTGCCATGA CCTCTACAGT 
CACCAATACC AGCTGCAGGA ACAACCACTA GAGGAACCAG GGCCCAGCAT TCGGAACTTG 
GATTTCTGGC CCAAACTTAT CACACTGATT GTATCAATCA TAGAGGAGGA TAAGAATTCC 
TACACACCTG TTCTGAACCA GTTTCCTCAG GAGTTGAATG TAGGAAAAGT CAGTGCAGAA 
GTGATGTGGC ACCTGTTTGC TCAAGACATG AAATATGCAC TGGAGGAACA TGAGAAAGAC 
CGGCTGTGTA AGAGTGCTGA CTACATGAAC CTGCACTTCA AGGTGAAGTG GCTCCACAAT 
GAATATGTGC GGGAGCTGCC TGCCCTCCAG GGGCAGGTGC CTGAGTACCC AGCGTGGTTT 
GAGCAGTTCG TGCTACAGTG GCTAGATGAA AATGAAGACG TGTCCCTGGA ATTCCTTCGT 
GGGGGCTTGG AACGAGATAA GAGAGATGGG TTCCAGCAAA CATCGGAGCA TGCCTTGTTT 
TCCTGTTCTG TGGTGGACGT CTTTACACAA CTCAACCAGA GCTTTGAGAT CATCCGGAAA 
TTGGAATGTC CAGACCCCAG CATCCTTGCC CATTATATGA GAAGGTTTGC TAAGACCATC 
GGCAAAGTGC TGATACAGTA TGCAGACATC TTATCAAAGA ACTTCCCAGC TTACTGCACA 
AAGGAGAGAC TGCCCTGTAT CCTGATGAAC AACATGCAGC AACTGAGGGT CCAGCTGGAG 
AAAATGTTTG AGGCCATGGG AGGCAAAGAG CTGGACTCTG AAGCTGCAGA CAGTCTGAAG 
GAGCTGCAGG TGAAACTGAA TACAGTTCTG GATGAGCTCA GCATGGTGTT TGGAAACAGC 
TTCCAGGTGC GGATCGACGA GTGTGTTCGA CAAATGGCTG ACATCCTGGG CCAAGTACGG 
GGCACAGGGA ATGCATCCCC TAACGCCAGA GCCTCGGTGG CTCAGGATGC AGATAGTGTG 
CTGCGACCTC TCATGGATTT CCTGGATGGC AACCTCACAC TGTTTGCCAC TGTGTGTGAG 
AAGACAGTGC TGAAGCGAGT CCTGAAGGAG CTCTGGCGAG TGGTGATGAA CACCATGGAG 
CGGGTCATCG TCCTGCCCCC ACTCATTGAC CAGACAGGCA CCCAGCTGAT CTTAACTGCT 
GCCAAGGAGC TGAGCCAGCT TTCCAAACTC AAGGACCACA TGGTACGAGA GGAAACACGG 
AATCTCACTC CAAAGCAGTG TGCTGTCCTC GACTTGGCCT TGGACACCGT CAAGCAATAC 
TTCCATGCAG GAGGCAATGG TCTGAAGAAA ACCTTCCTGG AAAAGAGCCC AGACCTGCAG 
TCTCTACGTT ATGCCCTCTC TCTGTATACA CAGACCACAG ACACTCTCAT CAAGACATTT 
GTGCGCTCAC AGACTGCCCA AGTACATGAT GGGAAAGGTA TTAGGTTTAC TGCTAACGAG 
GACATTCGGC CAGAAAAGGG GGCTGGTGTG GACGACCCTG TGGGAGAAGT CTCTATTCAG 
GTGGACTTGT TTACACATCC TGGCACTGGG GAGCATAAGG TCACAGTGAA AGTGGTAGCA 
GCCAATGATC TCAAGTGGCA GACAGCAGGT ATGTTCCGGC CTTTTGTGGA AGTGACCATG 
GTTGGCCCAC ACCAAAGCGA TAAGAAGAGG AAGTTCACAA CCAAGTCTAA AAGTAACAGC 
TGGACTCCCA AGTACAACGA GACTTTTCAT TTCCTCCTGG GAAATGAAGA GGGTCCCGAG 
GCCTATGAAT TGCAGATATG CGTGAAGGAT TACTGCTTTG CCCGGGAGGA TCGTGTGATA 
GGACTGGCAG TGATGCCCCT GAGGGATGTG GCAGCTAAGG GCAGCTGTGC CTGCTGGTGC 
CCGTTGGGCC GGAAGATCCA CATGGATGAG ACAGGCATGA CCATTCTCCG GATTCTGTCT 
CAGAGGAGCA ATGATGAGGT AGCCCGAGAG TTCGTGAAGC TCAAGTCAGA GTCTCGATCC 
ATAGAGGAAG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13b ( NM_001042579.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001036044.1 Rattus norvegicus unc-13 homolog B (Unc13b), transcript variant 2, mRNA.

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CloneID	ORa09925
Clone ID Related Accession (Same CDS sequence)	NM_001042579.1
Accession Version	NM_001042579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568217600000
Organism	Rattus norvegicus(Norway rat)
Product	protein unc-13 homolog B isoform u
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF159706.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF159706.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCGCTGC TCTGCGTGCG TGTTAAGAGA GCCAAATTCC AGGGTTCACC AGATAAGTTT 
AACACATACG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA AGATTTCATG TTTGAGATCA GCCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA AGGGCTGATA TGGGACACAA TGGTGGGGAC CGTGTGGATC 
GCGCTGAAGA CTATCCGCCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC TACACTGGAG 
GCTGAGACAT TAATGAAAAA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAA 
ATCTTGCTTG GTACACGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATAAATGCC TTGGCAGATG ATAATGAGTA TTCCAGTCAA 
GAAGAAAGCC AGAGGAAGCT GTTGCCCACT GCTGCCGCCC AGTGTCGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGGGAC 
AGTGACTATC GAAGTGAGAC CAGCAATAGT GCCCCACCTC CTTATCACAC GACTACCCAG 
CCCAACGCGT CTGCACACCA GTTCCCTATG CCAGTGCCAC TGCCACAGCA GCTATTTCTT 
CAGGGAAGTT CCCGAGACTC CTGCAATGAC TCTATGCAGA GTTATGACCT TGATTACCCA 
GAGCGTCGGG CCCTCAGCCC CACTAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CGAGGAAGCT CTCTGCTGAG TGAACTGGAC CAGTATCATG AGCAAGACGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGCCATAGC TATGGCAGCC TCTCCAGAGA TGGCCAGGCT 
GGCTTGGGAG AACAAGAGAA AGCCTTGGAA GTGGCCTGTG AATCACAGAA GGAGAAAACA 
GGTGAATCCA AGGAGAGAGA TGATGCCACA GTCTGCCCTC CGTCAGACCT GATGCTGCAC 
AAGGACCTCA CCCTAGGACC CCAGGAGAGT TTTCCCGAGG AGAAAGCATC GTCGCCCTTT 
ACCCAGGCCA GAGCACACTG GTTCCGAGCG GTTACCAAGG TCCGACTCCA GCTGCAGGAG 
ATTTCAGATG ATGGTGACCC CTCCTTGCCT CAGTGGCTCC CAGAAGGGCC AGCGGGAGGG 
CTCTATGGCA TCGACAGCAT GCCAGATCTA CGAAGAAAGA AGCCACTGCC ACTTGTCAGT 
GATCTGGCTA TGTCACTGGT CCAGTCACGG AAAGCAGGGA TTACTTCCGC TATGGCTACA 
CGTACCTCTC TCAAGGATGA AGATCTGAAA TCCCATGTGT ATAAGAAAAC TCTGCAGGCC 
TTAATCTACC CCATCTCGTG CACCACGCCC CACAACTTTG AGGTCTGGTC TGCCACGACA 
CCCACCTACT GCTATGAGTG TGAAGGCTTG CTCTGGGGCC TTGCCCGGCA AGGCATGCGC 
TGCAGCGAGT GTGGCGTCAA GTGCCATGAG AAGTGCCAAG ACCTGCTCAA TGCCGATTGC 
CTACAGCGGG CCGCTGAAAA GAGCTCTAAG CACGGAGCTG AGGACCGGAC TCAGAACATT 
ATCATGGCCA TGAAGGACCG TATGAAGATC CGAGAACGGA ATAAACCAGA GATCTTTGAG 
GTTATCCGGG ATGTGTTCAC AGTGAGCAAA GTTGCCCACG TGCAGCAGAT GAAAACAGTA 
AAGCAGAGTG TCCTGGATGG CACCTCCAAG TGGTCGGCCA AAATTACCAT CACGGTGGTT 
TGTGCCCAGG GCCTACAAGC CAAAGACAAG ACAGGATCCA GTGACCCTTA TGTGACTGTG 
CAAGTTGGGA AAACCAAGAA GCGCACTAAG ACCATTTTTG GAAATTTGAA TCCTGTTTGG 
GAGGAGAAGT TCCATTTTGA GTGCCACAAC TCTTCCGATC GAATTAAGGT ACGTGTGTGG 
GATGAGGATG ATGACATCAA ATCCAGAGTA AAGCAGCGGC TGAAGCGAGA GTCGGATGAT 
TTCCTTGGCC AAACTATCAT TGAAGTTCGA ACGCTGAGTG GAGAGATGGA TGTCTGGTAT 
AATCTGGAGA AGAGAACAGA CAAGTCAGCT GTCTCAGGGG CTATCCGACT GCAAATCAAT 
GTGGAGATCA AGGGGGAGGA GAAGGTAGCC CCATACCATG TGCAATACAC ATGTCTCCAT 
GAGAATCTTT TCCATTACCT CACAGACATT CAGGGCAGTG GAGGAGTCTG GATCCCAGAC 
GCTCGGGGGG ACGATGCATG GAAGGTGTAC TTTGACGAGA CAGCCCAAGA AATTGTGGAT 
GAATTTGCCA TGCGCTATGG CATCGAGTCC ATATATCAGG CCATGACGCA CTTTGCGTGT 
TTGTCATCCA AGTACATGTG TCCTGGCGTA CCAGCCGTGA TGAGTACCTT ACTGGCCAAC 
ATCAATGCGT ATTATGCTCA CACAACCGCC TCCACTAACG TCTCTGCATC TGACCGCTTT 
GCCGCCTCCA ACTTTGGTAA AGAAAGATTT GTAAAACTGC TGGACCAGCT ACACAACTCA 
CTGAGGATAG ACCTGTCTAC ATACAGGAAT AACTTTCCTG CTGGGAGCCC TGAGCGGCTC 
CAGGATTTAA AATCTACAGT GGACTTGCTG ACCAGCATTA CTTTCTTCAG GATGAAGGTG 
CAAGAACTTC AGAGCCCCCC AAGAGCCAGC CAGGTGGTAA AGGACTGTGT GAAGGCCTGC 
TTGAACTCTA CATATGAATA CATCTTCAAC AACTGCCATG ACCTCTACAG TCACCAATAC 
CAGCTGCAGG AACAACCACT AGAGGAACCA GGGCCCAGCA TTCGGAACTT GGATTTCTGG 
CCCAAACTTA TCACACTGAT TGTATCAATC ATAGAGGAGG ATAAGAATTC CTACACACCT 
GTTCTGAACC AGTTTCCTCA GGAGTTGAAT GTAGGAAAAG TCAGTGCAGA AGTGATGTGG 
CACCTGTTTG CTCAAGACAT GAAATATGCA CTGGAGGAAC ATGAGAAAGA CCGGCTGTGT 
AAGAGTGCTG ACTACATGAA CCTGCACTTC AAGGTGAAGT GGCTCCACAA TGAATATGTG 
CGGGAGCTGC CTGCCCTCCA GGGGCAGGTG CCTGAGTACC CAGCGTGGTT TGAGCAGTTC 
GTGCTACAGT GGCTAGATGA AAATGAAGAC GTGTCCCTGG AATTCCTTCG TGGGGGCTTG 
GAACGAGATA AGAGAGATGG GTTCCAGCAA ACATCGGAGC ATGCCTTGTT TTCCTGTTCT 
GTGGTGGACG TCTTTACACA ACTCAACCAG AGCTTTGAGA TCATCCGGAA ATTGGAATGT 
CCAGACCCCA GCATCCTTGC CCATTATATG AGAAGGTTTG CTAAGACCAT CGGCAAAGTG 
CTGATACAGT ATGCAGACAT CTTATCAAAG AACTTCCCAG CTTACTGCAC AAAGGAGAGA 
CTGCCCTGTA TCCTGATGAA CAACATGCAG CAACTGAGGG TCCAGCTGGA GAAAATGTTT 
GAGGCCATGG GAGGCAAAGA GCTGGACTCT GAAGCTGCAG ACAGTCTGAA GGAGCTGCAG 
GTGAAACTGA ATACAGTTCT GGATGAGCTC AGCATGGTGT TTGGAAACAG CTTCCAGGTG 
CGGATCGACG AGTGTGTTCG ACAAATGGCT GACATCCTGG GCCAAGTACG GGGCACAGGG 
AATGCATCCC CTAACGCCAG AGCCTCGGTG GCTCAGGATG CAGATAGTGT GCTGCGACCT 
CTCATGGATT TCCTGGATGG CAACCTCACA CTGTTTGCCA CTGTGTGTGA GAAGACAGTG 
CTGAAGCGAG TCCTGAAGGA GCTCTGGCGA GTGGTGATGA ACACCATGGA GCGGGTCATC 
GTCCTGCCCC CACTCATTGA CCAGACAGGC ACCCAGCTGA TCTTAACTGC TGCCAAGGAG 
CTGAGCCAGC TTTCCAAACT CAAGGACCAC ATGGTACGAG AGGAAACACG GAATCTCACT 
CCAAAGCAGT GTGCTGTCCT CGACTTGGCC TTGGACACCG TCAAGCAATA CTTCCATGCA 
GGAGGCAATG GTCTGAAGAA AACCTTCCTG GAAAAGAGCC CAGACCTGCA GTCTCTACGT 
TATGCCCTCT CTCTGTATAC ACAGACCACA GACACTCTCA TCAAGACATT TGTGCGCTCA 
CAGACTGCCC AAGTACATGA TGGGAAAGGT ATTAGGTTTA CTGCTAACGA GGACATTCGG 
CCAGAAAAGG GGGCTGGTGT GGACGACCCT GTGGGAGAAG TCTCTATTCA GGTGGACTTG 
TTTACACATC CTGGCACTGG GGAGCATAAG GTCACAGTGA AAGTGGTAGC AGCCAATGAT 
CTCAAGTGGC AGACAGCAGG TATGTTCCGG CCTTTTGTGG AAGTGACCAT GGTTGGCCCA 
CACCAAAGCG ATAAGAAGAG GAAGTTCACA ACCAAGTCTA AAAGTAACAG CTGGACTCCC 
AAGTACAACG AGACTTTTCA TTTCCTCCTG GGAAATGAAG AGGGTCCCGA GGCCTATGAA 
TTGCAGATAT GCGTGAAGGA TTACTGCTTT GCCCGGGAGG ATCGTGTGAT AGGACTGGCA 
GTGATGCCCC TGAGGGATGT GGCAGCTAAG GGCAGCTGTG CCTGCTGGTG CCCGTTGGGC 
CGGAAGATCC ACATGGATGA GACAGGCATG ACCATTCTCC GGATTCTGTC TCAGAGGAGC 
AATGATGAGG TAGCCCGAGA GTTCGTGAAG CTCAAGTCAG AGTCTCGATC CATAGAGGAA 
GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036044.1
CDS	101..4969
Translation	MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKNDEICGTKNPTPHKILLGTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKLLPTAAAQCRHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASAHQFPMPVPLPQQLFLQGSSRDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSRGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSRDGQAGLGEQEKALEVACESQKEKTGESKERDDATVCPPSDLMLHKDLTLGPQESFPEEKASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLAMSLVQSRKAGITSAMATRTSLKDEDLKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQINVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPDARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRELPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGGLERDKRDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLIQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLIDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTVKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNSWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSIEEGS

Target ORF information:

RefSeq Version	NM_001042579.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus unc-13 homolog B (Unc13b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042579.1

ORF Insert Sequence:

ATGTCGCTGC TCTGCGTGCG TGTTAAGAGA GCCAAATTCC AGGGTTCACC AGATAAGTTT 
AACACATACG TGACCCTGAA AGTCCAGAAC GTGAAGAGCA CAACTGTAGC AGTTCGTGGT 
GATCAGCCTT CCTGGGAGCA AGATTTCATG TTTGAGATCA GCCGTCTGGA CCTGGGCCTA 
AGTGTGGAGG TGTGGAACAA AGGGCTGATA TGGGACACAA TGGTGGGGAC CGTGTGGATC 
GCGCTGAAGA CTATCCGCCA GTCGGATGAG GAAGGGCCTG GGGAGTGGTC TACACTGGAG 
GCTGAGACAT TAATGAAAAA TGATGAGATC TGTGGAACTA AAAACCCAAC TCCTCATAAA 
ATCTTGCTTG GTACACGATT TGAGCTGCCT TTTGACATCC CAGAGGAGGA AGCCAGATAT 
TGGACCTACA AATTGGAACA AATAAATGCC TTGGCAGATG ATAATGAGTA TTCCAGTCAA 
GAAGAAAGCC AGAGGAAGCT GTTGCCCACT GCTGCCGCCC AGTGTCGTCA CTGGACCTAT 
TTGGGCTGGG GAGAGCATCA GACCTTTGAA GACCCTGATA GTGCCGTTGA TGACCGGGAC 
AGTGACTATC GAAGTGAGAC CAGCAATAGT GCCCCACCTC CTTATCACAC GACTACCCAG 
CCCAACGCGT CTGCACACCA GTTCCCTATG CCAGTGCCAC TGCCACAGCA GCTATTTCTT 
CAGGGAAGTT CCCGAGACTC CTGCAATGAC TCTATGCAGA GTTATGACCT TGATTACCCA 
GAGCGTCGGG CCCTCAGCCC CACTAGCAGT AGTAGGTATG GCTCCTCCTG TAATGTGAGT 
CGAGGAAGCT CTCTGCTGAG TGAACTGGAC CAGTATCATG AGCAAGACGA TGATGGTCGG 
GAGAGGGACT CCATTCATTC TAGCCATAGC TATGGCAGCC TCTCCAGAGA TGGCCAGGCT 
GGCTTGGGAG AACAAGAGAA AGCCTTGGAA GTGGCCTGTG AATCACAGAA GGAGAAAACA 
GGTGAATCCA AGGAGAGAGA TGATGCCACA GTCTGCCCTC CGTCAGACCT GATGCTGCAC 
AAGGACCTCA CCCTAGGACC CCAGGAGAGT TTTCCCGAGG AGAAAGCATC GTCGCCCTTT 
ACCCAGGCCA GAGCACACTG GTTCCGAGCG GTTACCAAGG TCCGACTCCA GCTGCAGGAG 
ATTTCAGATG ATGGTGACCC CTCCTTGCCT CAGTGGCTCC CAGAAGGGCC AGCGGGAGGG 
CTCTATGGCA TCGACAGCAT GCCAGATCTA CGAAGAAAGA AGCCACTGCC ACTTGTCAGT 
GATCTGGCTA TGTCACTGGT CCAGTCACGG AAAGCAGGGA TTACTTCCGC TATGGCTACA 
CGTACCTCTC TCAAGGATGA AGATCTGAAA TCCCATGTGT ATAAGAAAAC TCTGCAGGCC 
TTAATCTACC CCATCTCGTG CACCACGCCC CACAACTTTG AGGTCTGGTC TGCCACGACA 
CCCACCTACT GCTATGAGTG TGAAGGCTTG CTCTGGGGCC TTGCCCGGCA AGGCATGCGC 
TGCAGCGAGT GTGGCGTCAA GTGCCATGAG AAGTGCCAAG ACCTGCTCAA TGCCGATTGC 
CTACAGCGGG CCGCTGAAAA GAGCTCTAAG CACGGAGCTG AGGACCGGAC TCAGAACATT 
ATCATGGCCA TGAAGGACCG TATGAAGATC CGAGAACGGA ATAAACCAGA GATCTTTGAG 
GTTATCCGGG ATGTGTTCAC AGTGAGCAAA GTTGCCCACG TGCAGCAGAT GAAAACAGTA 
AAGCAGAGTG TCCTGGATGG CACCTCCAAG TGGTCGGCCA AAATTACCAT CACGGTGGTT 
TGTGCCCAGG GCCTACAAGC CAAAGACAAG ACAGGATCCA GTGACCCTTA TGTGACTGTG 
CAAGTTGGGA AAACCAAGAA GCGCACTAAG ACCATTTTTG GAAATTTGAA TCCTGTTTGG 
GAGGAGAAGT TCCATTTTGA GTGCCACAAC TCTTCCGATC GAATTAAGGT ACGTGTGTGG 
GATGAGGATG ATGACATCAA ATCCAGAGTA AAGCAGCGGC TGAAGCGAGA GTCGGATGAT 
TTCCTTGGCC AAACTATCAT TGAAGTTCGA ACGCTGAGTG GAGAGATGGA TGTCTGGTAT 
AATCTGGAGA AGAGAACAGA CAAGTCAGCT GTCTCAGGGG CTATCCGACT GCAAATCAAT 
GTGGAGATCA AGGGGGAGGA GAAGGTAGCC CCATACCATG TGCAATACAC ATGTCTCCAT 
GAGAATCTTT TCCATTACCT CACAGACATT CAGGGCAGTG GAGGAGTCTG GATCCCAGAC 
GCTCGGGGGG ACGATGCATG GAAGGTGTAC TTTGACGAGA CAGCCCAAGA AATTGTGGAT 
GAATTTGCCA TGCGCTATGG CATCGAGTCC ATATATCAGG CCATGACGCA CTTTGCGTGT 
TTGTCATCCA AGTACATGTG TCCTGGCGTA CCAGCCGTGA TGAGTACCTT ACTGGCCAAC 
ATCAATGCGT ATTATGCTCA CACAACCGCC TCCACTAACG TCTCTGCATC TGACCGCTTT 
GCCGCCTCCA ACTTTGGTAA AGAAAGATTT GTAAAACTGC TGGACCAGCT ACACAACTCA 
CTGAGGATAG ACCTGTCTAC ATACAGGAAT AACTTTCCTG CTGGGAGCCC TGAGCGGCTC 
CAGGATTTAA AATCTACAGT GGACTTGCTG ACCAGCATTA CTTTCTTCAG GATGAAGGTG 
CAAGAACTTC AGAGCCCCCC AAGAGCCAGC CAGGTGGTAA AGGACTGTGT GAAGGCCTGC 
TTGAACTCTA CATATGAATA CATCTTCAAC AACTGCCATG ACCTCTACAG TCACCAATAC 
CAGCTGCAGG AACAACCACT AGAGGAACCA GGGCCCAGCA TTCGGAACTT GGATTTCTGG 
CCCAAACTTA TCACACTGAT TGTATCAATC ATAGAGGAGG ATAAGAATTC CTACACACCT 
GTTCTGAACC AGTTTCCTCA GGAGTTGAAT GTAGGAAAAG TCAGTGCAGA AGTGATGTGG 
CACCTGTTTG CTCAAGACAT GAAATATGCA CTGGAGGAAC ATGAGAAAGA CCGGCTGTGT 
AAGAGTGCTG ACTACATGAA CCTGCACTTC AAGGTGAAGT GGCTCCACAA TGAATATGTG 
CGGGAGCTGC CTGCCCTCCA GGGGCAGGTG CCTGAGTACC CAGCGTGGTT TGAGCAGTTC 
GTGCTACAGT GGCTAGATGA AAATGAAGAC GTGTCCCTGG AATTCCTTCG TGGGGGCTTG 
GAACGAGATA AGAGAGATGG GTTCCAGCAA ACATCGGAGC ATGCCTTGTT TTCCTGTTCT 
GTGGTGGACG TCTTTACACA ACTCAACCAG AGCTTTGAGA TCATCCGGAA ATTGGAATGT 
CCAGACCCCA GCATCCTTGC CCATTATATG AGAAGGTTTG CTAAGACCAT CGGCAAAGTG 
CTGATACAGT ATGCAGACAT CTTATCAAAG AACTTCCCAG CTTACTGCAC AAAGGAGAGA 
CTGCCCTGTA TCCTGATGAA CAACATGCAG CAACTGAGGG TCCAGCTGGA GAAAATGTTT 
GAGGCCATGG GAGGCAAAGA GCTGGACTCT GAAGCTGCAG ACAGTCTGAA GGAGCTGCAG 
GTGAAACTGA ATACAGTTCT GGATGAGCTC AGCATGGTGT TTGGAAACAG CTTCCAGGTG 
CGGATCGACG AGTGTGTTCG ACAAATGGCT GACATCCTGG GCCAAGTACG GGGCACAGGG 
AATGCATCCC CTAACGCCAG AGCCTCGGTG GCTCAGGATG CAGATAGTGT GCTGCGACCT 
CTCATGGATT TCCTGGATGG CAACCTCACA CTGTTTGCCA CTGTGTGTGA GAAGACAGTG 
CTGAAGCGAG TCCTGAAGGA GCTCTGGCGA GTGGTGATGA ACACCATGGA GCGGGTCATC 
GTCCTGCCCC CACTCATTGA CCAGACAGGC ACCCAGCTGA TCTTAACTGC TGCCAAGGAG 
CTGAGCCAGC TTTCCAAACT CAAGGACCAC ATGGTACGAG AGGAAACACG GAATCTCACT 
CCAAAGCAGT GTGCTGTCCT CGACTTGGCC TTGGACACCG TCAAGCAATA CTTCCATGCA 
GGAGGCAATG GTCTGAAGAA AACCTTCCTG GAAAAGAGCC CAGACCTGCA GTCTCTACGT 
TATGCCCTCT CTCTGTATAC ACAGACCACA GACACTCTCA TCAAGACATT TGTGCGCTCA 
CAGACTGCCC AAGTACATGA TGGGAAAGGT ATTAGGTTTA CTGCTAACGA GGACATTCGG 
CCAGAAAAGG GGGCTGGTGT GGACGACCCT GTGGGAGAAG TCTCTATTCA GGTGGACTTG 
TTTACACATC CTGGCACTGG GGAGCATAAG GTCACAGTGA AAGTGGTAGC AGCCAATGAT 
CTCAAGTGGC AGACAGCAGG TATGTTCCGG CCTTTTGTGG AAGTGACCAT GGTTGGCCCA 
CACCAAAGCG ATAAGAAGAG GAAGTTCACA ACCAAGTCTA AAAGTAACAG CTGGACTCCC 
AAGTACAACG AGACTTTTCA TTTCCTCCTG GGAAATGAAG AGGGTCCCGA GGCCTATGAA 
TTGCAGATAT GCGTGAAGGA TTACTGCTTT GCCCGGGAGG ATCGTGTGAT AGGACTGGCA 
GTGATGCCCC TGAGGGATGT GGCAGCTAAG GGCAGCTGTG CCTGCTGGTG CCCGTTGGGC 
CGGAAGATCC ACATGGATGA GACAGGCATG ACCATTCTCC GGATTCTGTC TCAGAGGAGC 
AATGATGAGG TAGCCCGAGA GTTCGTGAAG CTCAAGTCAG AGTCTCGATC CATAGAGGAA 
GGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.
The Journal of cell biology216(4)1143-1161(2017 04)
Kawabe H,Mitkovski M,Kaeser PS,Hirrlinger J,Opazo F,Nestvogel D,Kalla S,Fejtova A,Verrier SE,Bungers SR,Cooper BH,Varoqueaux F,Wang Y,Nehring RB,Gundelfinger ED,Rosenmund C,Rizzoli SO,Südhof TC,Rhee JS,Brose N

Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis.
eLife4(2015 Nov)
Man KN,Imig C,Walter AM,Pinheiro PS,Stevens DR,Rettig J,Sørensen JB,Cooper BH,Brose N,Wojcik SM

The morphological and molecular nature of synaptic vesicle priming at presynaptic active zones.
Neuron84(2)416-31(2014 Oct)
Imig C,Min SW,Krinner S,Arancillo M,Rosenmund C,Südhof TC,Rhee J,Brose N,Cooper BH

CAPS and Munc13 utilize distinct PIP2-linked mechanisms to promote vesicle exocytosis.
Molecular biology of the cell25(4)508-21(2014 Feb)
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The Munc13 proteins differentially regulate readily releasable pool dynamics and calcium-dependent recovery at a central synapse.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(19)8336-51(2013 May)
Chen Z,Cooper B,Kalla S,Varoqueaux F,Young SM Jr

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