Ppp2r3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp2r3a
Entrez Gene ID	363122
Full Name	protein phosphatase 2, regulatory subunit B'', alpha
General protein information	Preferred Names protein phosphatase 2, regulatory subunit B'', alpha Names serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha DNA for thyroid hormone receptor binding site (258bp) protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243634.3	XP_006243696.1	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha isoform X2
NM_001012202.2	NP_001012202.2	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha
XM_006243633.3	XP_006243695.1	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ppp2r3a	NP_001154834.1
Arabidopsis thaliana (thale cress)	AT1G54450	NP_175847.1
Oryza sativa (rice)	Os10g0476600	NP_001064850.1
Homo sapiens (human)	PPP2R3A	NP_002709.2
Macaca mulatta (Rhesus monkey)	PPP2R3A	XP_001115154.1
Caenorhabditis elegans (roundworm)	C06G1.5	NP_001257276.1
Canis lupus familiaris (dog)	PPP2R3A	XP_003639877.1
Bos taurus (cattle)	LOC787607	XP_005201978.1
Arabidopsis thaliana (thale cress)	AT5G28850	NP_568509.1
Xenopus tropicalis (tropical clawed frog)	ppp2r3a	XP_002939965.2
Pan troglodytes (chimpanzee)	PPP2R3A	XP_001153482.1
Danio rerio (zebrafish)	LOC565849	XP_005163462.1
Rattus norvegicus (Norway rat)	Ppp2r3a	NP_001012202.2
Gallus gallus (chicken)	PPP2R3A	XP_422556.4
Arabidopsis thaliana (thale cress)	AT5G28900	NP_198242.1

Gene Ontology
Bibliography

Related articles in PubMed

DARPP-32, Jack of All Trades… Master of Which?
Yger M, Girault JA
Frontiers in behavioral neuroscience556(2011)

Structure of protein phosphatase 2A core enzyme bound to tumor-inducing toxins.
Xing Y, Xu Y, Chen Y, Jeffrey PD, Chao Y, Lin Z, Li Z, Strack S, Stock JB, Shi Y
Cell127(2)341-53(2006 Oct)

PR130 is a modulator of the Wnt-signaling cascade that counters repression of the antagonist Naked cuticle.
Creyghton MP, Roël G, Eichhorn PJ, Vredeveld LC, Destrée O, Bernards R
Proceedings of the National Academy of Sciences of the United States of America103(14)5397-402(2006 Apr)

PR72, a novel regulator of Wnt signaling required for Naked cuticle function.
Creyghton MP, Roël G, Eichhorn PJ, Hijmans EM, Maurer I, Destrée O, Bernards R
Genes & development19(3)376-86(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppp2r3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp2r3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa31499	XM_006243634.3 Latest version!	Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa02681	NM_001012202.2 Latest version!	Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa02681	XM_006243633.3 Latest version!	Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp2r3a ( XM_006243634.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243696.1 Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa31499
Clone ID Related Accession (Same CDS sequence)	XM_006243634.3
Accession Version	XM_006243634.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATCA AGGAGACATC TTTGCGAAGG GACCCGGACT TAAGGGGAGA GTTAGCGTTC 
CTAGCAAGGG GCTGTGATTT TGTCCTCCCA TCACGGTTTA AGAAGAGGCT GAAGTCATTT 
CAGCAGACAC AGATTCAAAA TAAACCAGAA AAAAAACCTG CAACACCATG TCCACCTGCA 
GCCACCCTTC CAAGTAGTCC CCAGCCTGCC TCCCCTGTTC CTTGTGTGAG CGATGTCGTG 
AATACACCAC TGTCCATAAA CATTCCACAG TTCTACTTTC CCGAAGGACT CCCAGACACC 
TGCAGCAACC ACGAACAGAT CCTAGGCAGA ATTGAAGCTG CTTTCAAGGA TATTGAAGAT 
CAAAAAGCAG ATATTCATGA AATGGGGAGA ATTGCAAAGG CCTGCGGCTG TCCACTCTAT 
TGGAAAGCCC CCATGTTCAG GGCTGCAGGA GGAGAGAAGA CTGGATTTGT GACAGCACAG 
TCATTCATGG CCATGTGGAA AAAGTTGCTA AGTGACCATC ATGATGATGC CTCCAGGTTC 
ATCTGGCTTC TGGCAAAGCC CAGCTGCAGC CATCTCCAAC AAGAGGATTT TATCCCTCTA 
CTTCAGGATG TGGTAGATAC ACACCCTGGC CTCACCTTCC TGAAAGATGC TCCTGAATTC 
CATTCCCGCT ACATCACCAC GGTTGTTCAG AGAATATTCT ACACAGTCAA CAGATCTTGG 
AGTGGAAAAA TTACTTCAAC AGAGATAAGG AAAAGCAACT TTTTGCAAAC TCTGGCCCTT 
TTGGAGGAAG AGGAAGATAT AAACCAAATC ACAGATTACT TCTCTTATGA ACATTTCTAT 
GTCATTTATT GTAAATTCTG GGAACTAGAT GGTGATCATG ACCTATACAT CAGCCAAGCT 
GATCTCTCTC GATACAATGA CCAGGCTTCA TCAAATAGAA TTATTGAAAG AATATTTTCT 
GGAGCAGTAA CAAGAGGGAA AACTGTACAA AGAGAGGGAA GAATGAGCTA TGCAGATTTT 
GTTTGGTTTT TGATTTCTGA GGAAGACAAA CGGAACCCTA CCAGCATTGA GTATTGGTTC 
CGCTGCATGG ATGTGGATGG AGATGGTGTA CTTTCCATGT ATGAACTAGA GTATTTCTAT 
GAAGAGCAGT GTGAACGGAT GGAAGCCATG GGCATCGAGC CCTTGCCGTT TCATGATCTG 
TTGTGCCAGA TGCTAGACTT GGTGAAGCCA GCTAATGATG GCAGAATAAC TCTGCGAGAT 
TTGAAGAGGT GCAGAATGGC TCACATCTTC TACGACACTT TCTTTAACCT GGAGAAGTAC 
TTGGACCATG AACAAAGAGA TCCTTTTGCA GTCCAGAAGG ATGTTGAAAA TGAGGGCCCC 
GAGCCCTCTG ACTGGGACAG GTTTGCTGCT GAGGAGTACG AGGCTCTGGT GACTGAGGAA 
TCTGCCCAGG TTCAGCTCCA GGAAGGCTCA TTTGAAGACT ATGAATCAGA TGAGCCTGTG 
TCTCCCTCTG AACTTGGAAA CAAGGGCAAT AAAATCCTAA CTTCAAGCCT TTCAGAGAAA 
TGTGGAAAGC TCCAGTCAGT GGATGAAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243696.1
CDS	887..2479
Translation	MMIKETSLRRDPDLRGELAFLARGCDFVLPSRFKKRLKSFQQTQIQNKPEKKPATPCPPAATLPSSPQPASPVPCVSDVVNTPLSINIPQFYFPEGLPDTCSNHEQILGRIEAAFKDIEDQKADIHEMGRIAKACGCPLYWKAPMFRAAGGEKTGFVTAQSFMAMWKKLLSDHHDDASRFIWLLAKPSCSHLQQEDFIPLLQDVVDTHPGLTFLKDAPEFHSRYITTVVQRIFYTVNRSWSGKITSTEIRKSNFLQTLALLEEEEDINQITDYFSYEHFYVIYCKFWELDGDHDLYISQADLSRYNDQASSNRIIERIFSGAVTRGKTVQREGRMSYADFVWFLISEEDKRNPTSIEYWFRCMDVDGDGVLSMYELEYFYEEQCERMEAMGIEPLPFHDLLCQMLDLVKPANDGRITLRDLKRCRMAHIFYDTFFNLEKYLDHEQRDPFAVQKDVENEGPEPSDWDRFAAEEYEALVTEESAQVQLQEGSFEDYESDEPVSPSELGNKGNKILTSSLSEKCGKLQSVDEE

Target ORF information:

RefSeq Version	XM_006243634.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243634.3

ORF Insert Sequence:

ATGATGATCA AGGAGACATC TTTGCGAAGG GACCCGGACT TAAGGGGAGA GTTAGCGTTC 
CTAGCAAGGG GCTGTGATTT TGTCCTCCCA TCACGGTTTA AGAAGAGGCT GAAGTCATTT 
CAGCAGACAC AGATTCAAAA TAAACCAGAA AAAAAACCTG CAACACCATG TCCACCTGCA 
GCCACCCTTC CAAGTAGTCC CCAGCCTGCC TCCCCTGTTC CTTGTGTGAG CGATGTCGTG 
AATACACCAC TGTCCATAAA CATTCCACAG TTCTACTTTC CCGAAGGACT CCCAGACACC 
TGCAGCAACC ACGAACAGAT CCTAGGCAGA ATTGAAGCTG CTTTCAAGGA TATTGAAGAT 
CAAAAAGCAG ATATTCATGA AATGGGGAGA ATTGCAAAGG CCTGCGGCTG TCCACTCTAT 
TGGAAAGCCC CCATGTTCAG GGCTGCAGGA GGAGAGAAGA CTGGATTTGT GACAGCACAG 
TCATTCATGG CCATGTGGAA AAAGTTGCTA AGTGACCATC ATGATGATGC CTCCAGGTTC 
ATCTGGCTTC TGGCAAAGCC CAGCTGCAGC CATCTCCAAC AAGAGGATTT TATCCCTCTA 
CTTCAGGATG TGGTAGATAC ACACCCTGGC CTCACCTTCC TGAAAGATGC TCCTGAATTC 
CATTCCCGCT ACATCACCAC GGTTGTTCAG AGAATATTCT ACACAGTCAA CAGATCTTGG 
AGTGGAAAAA TTACTTCAAC AGAGATAAGG AAAAGCAACT TTTTGCAAAC TCTGGCCCTT 
TTGGAGGAAG AGGAAGATAT AAACCAAATC ACAGATTACT TCTCTTATGA ACATTTCTAT 
GTCATTTATT GTAAATTCTG GGAACTAGAT GGTGATCATG ACCTATACAT CAGCCAAGCT 
GATCTCTCTC GATACAATGA CCAGGCTTCA TCAAATAGAA TTATTGAAAG AATATTTTCT 
GGAGCAGTAA CAAGAGGGAA AACTGTACAA AGAGAGGGAA GAATGAGCTA TGCAGATTTT 
GTTTGGTTTT TGATTTCTGA GGAAGACAAA CGGAACCCTA CCAGCATTGA GTATTGGTTC 
CGCTGCATGG ATGTGGATGG AGATGGTGTA CTTTCCATGT ATGAACTAGA GTATTTCTAT 
GAAGAGCAGT GTGAACGGAT GGAAGCCATG GGCATCGAGC CCTTGCCGTT TCATGATCTG 
TTGTGCCAGA TGCTAGACTT GGTGAAGCCA GCTAATGATG GCAGAATAAC TCTGCGAGAT 
TTGAAGAGGT GCAGAATGGC TCACATCTTC TACGACACTT TCTTTAACCT GGAGAAGTAC 
TTGGACCATG AACAAAGAGA TCCTTTTGCA GTCCAGAAGG ATGTTGAAAA TGAGGGCCCC 
GAGCCCTCTG ACTGGGACAG GTTTGCTGCT GAGGAGTACG AGGCTCTGGT GACTGAGGAA 
TCTGCCCAGG TTCAGCTCCA GGAAGGCTCA TTTGAAGACT ATGAATCAGA TGAGCCTGTG 
TCTCCCTCTG AACTTGGAAA CAAGGGCAAT AAAATCCTAA CTTCAAGCCT TTCAGAGAAA 
TGTGGAAAGC TCCAGTCAGT GGATGAAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp2r3a ( NM_001012202.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012202.2 Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), mRNA.

Price & Availability↑

CloneID	ORa02681
Clone ID Related Accession (Same CDS sequence)	NM_001012202.2 , XM_006243633.3
Accession Version	NM_001012202.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07071327.1 and AABR07071326.1. On Sep 13, 2008 this sequence version replaced NM_001012202.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCAA CTTACAGACT CGTGGTTAGC ACTGTGAACC ATTATAGCAG TGTAGTGATA 
GACCGGCGTT TTGAACAAGC CATACATTAT TGCACTGGAA CCTGCCATAC CTTCACACAT 
GGAATAGACT GCATTGTAGT GCATCATAGT GTTTGTGCAG ACCTTTTGCA CATCCCTGTG 
TCTCAGTTCA AAGATGTGGA TCTGAACTCT GTGTTTTTGT CTCATGAAAA TGGCCTTTCC 
TCAGCTGAAG ACTATCCCCA TCAGGCCCTC ACAGGAATAC CCAGGTCCAA GAGAGCATCC 
ACATTTCAGA ATACCTGTAA CTTAAAGGAC ATTGCAGGAG AAGCAATCAG CTTTGCCAGT 
GGGAAAATAA AAGAATTTTC CCTTGAAAAA CTCAGAAATT CAAATCATGC AGCTTACAAA 
AAAGGAAGAA AAGTTAAATC TGACTCATTT AATAGGCGAT CAGTTGATTT GGATTTACTC 
TGTGGCCATT ATAGTAACGA TGGGACCTCC CCATCCTTTG GCTTACTCCG GAGTTCCTCA 
GTTGAGGAAA AATCTTTATC TCATAGAAAC TCACTAGATA CAAGCCTAAC TTCCATGCTT 
TTTCAGAACT TGTCTGAAGA AGATCTAGTT ACGCAGATTT TGGAAAAACA TAAAATAGAT 
CATTTTTCTT CTGGGACAGA CATAAAAATG TGCTTGGACA TCTTACTGAA ATGCTCTGAA 
GATTTGAAAA AATGTACAGA CATCATAAAA CAATGCATAA AGAAAAAGTC AGGAGGAAGC 
ATCAACGAAG GAAGTGGCAG TGACGCAATG TCTAACTCTG AAACCGTCTA TATGAATGTA 
ATGGCCAGGT TAGCTTACTA CCTGAGAAAG TTACCATTTG AATTTGTGCA GTCTGGGAGT 
AATGAGACTG TAGATTTGAC AGAGCTTGTC AATAATGTAC CTAACTTACA ACTGACTCCA 
TTCTCCCCAT TATATGGGAC TGAACAGCCC CCTAAATATG AAGATATCGT CCACCTCTCA 
GCCCTTGACT CCAGACAGTT TCAGACTGTT GAATTGCAAG ATGACATGCA TAATTTTAAA 
CAAACAGATA CTGTAAAAAC CCCTCCAAAC AACACAAATT CCTTGTGTAG CTTGGAGGTA 
AACGATCCTG GAACTCTAAA ACCTGTGCAG CTTCAATCAC CGTCATTAAC CATAAACCAT 
TTAGAGAATG TTTCTTCTGG TAACTTAATG GAAACTCTTT ATATTGAAGA AGAGTCAGAT 
GGAAAGAAGT CATTATTAGA TAAAGGACAA AAAGCAGATA ATGGACAAGG TCAGGAACCA 
TTAAAGGTAA ATGAACATAA AGAATTTTCA GACCATGATA CTTATCTTAA AAAATGTCCT 
GCCCCAGTGC AAAATGAAAT CAGTAAGATA TTTGAAAAGC CAATGGTAGT TCGTCCACCT 
AAGGAAGAAT CTGAATTAGC AGTTCCTCCT ACAGAACTCA GAGCCCAGAG GCACTTTATG 
AATCCTAACA GTCAAGAAGA GATCGACAAA TTGTTAATGG ATTTGGAATC ATTCTCACAG 
AAGATGGAGA CCTCTCTAGG AGAACCACTT GCCAAGGGTA AAAGCATCAA TTCTCTAAAT 
AGTCATAGTC ATTTGACTGG TCAGATCCAC AAAGATCTTG AGCCTAAACC TATAGTCTCT 
TCAGCTACGG AGAAAGTCTC ACCTTCTTGT CTAACAAGGA TTATTGAAAA CAACGGACAC 
AAAATAGAGG AAGAGGACCG AGCCCTCTTA CTTCGAATTC TGGAAAGCAT TGAAGACTTT 
GCTCAAGAAC TAGTTGAATG CAAGTCAAGC AGAGGGAGCC TATCACAAGA AAAGGAAATG 
ATGCAAATTC TGCAGGAAAC TTTGACAGCT TCCTCCCAGC CCACTCCATC AGTCAGTAGG 
AGCCCTGTTG GTGAGAAACC CAAAGATAGT ACTTCAGTTG TTTTGATTCA ACAGACACCA 
GAAGTGATCA AGATTCAAAA TAAACCAGAA AAAAAACCTG CAACACCATG TCCACCTGCA 
GCCACCCTTC CAAGTAGTCC CCAGCCTGCC TCCCCTGTTC CTTGTGTGAG CGATGTCGTG 
AATACACCAC TGTCCATAAA CATTCCACAG TTCTACTTTC CCGAAGGACT CCCAGACACC 
TGCAGCAACC ACGAACAGAT CCTAGGCAGA ATTGAAGCTG CTTTCAAGGA TATTGAAGAT 
CAAAAAGCAG ATATTCATGA AATGGGGAGA ATTGCAAAGG CCTGCGGCTG TCCACTCTAT 
TGGAAAGCCC CCATGTTCAG GGCTGCAGGA GGAGAGAAGA CTGGATTTGT GACAGCACAG 
TCATTCATGG CCATGTGGAA AAAGTTGCTA AGTGACCATC ATGATGATGC CTCCAGGTTC 
ATCTGGCTTC TGGCAAAGCC CAGCTGCAGC CATCTCCAAC AAGAGGATTT TATCCCTCTA 
CTTCAGGATG TGGTAGATAC ACACCCTGGC CTCACCTTCC TGAAAGATGC TCCTGAATTC 
CATTCCCGCT ACATCACCAC GGTTGTTCAG AGAATATTCT ACACAGTCAA CAGATCTTGG 
AGTGGAAAAA TTACTTCAAC AGAGATAAGG AAAAGCAACT TTTTGCAAAC TCTGGCCCTT 
TTGGAGGAAG AGGAAGATAT AAACCAAATC ACAGATTACT TCTCTTATGA ACATTTCTAT 
GTCATTTATT GTAAATTCTG GGAACTAGAT GGTGATCATG ACCTATACAT CAGCCAAGCT 
GATCTCTCTC GATACAATGA CCAGGCTTCA TCAAATAGAA TTATTGAAAG AATATTTTCT 
GGAGCAGTAA CAAGAGGGAA AACTGTACAA AGAGAGGGAA GAATGAGCTA TGCAGATTTT 
GTTTGGTTTT TGATTTCTGA GGAAGACAAA CGGAACCCTA CCAGCATTGA GTATTGGTTC 
CGCTGCATGG ATGTGGATGG AGATGGTGTA CTTTCCATGT ATGAACTAGA GTATTTCTAT 
GAAGAGCAGT GTGAACGGAT GGAAGCCATG GGCATCGAGC CCTTGCCGTT TCATGATCTG 
TTGTGCCAGA TGCTAGACTT GGTGAAGCCA GCTAATGATG GCAGAATAAC TCTGCGAGAT 
TTGAAGAGGT GCAGAATGGC TCACATCTTC TACGACACTT TCTTTAACCT GGAGAAGTAC 
TTGGACCATG AACAAAGAGA TCCTTTTGCA GTCCAGAAGG ATGTTGAAAA TGAGGGCCCC 
GAGCCCTCTG ACTGGGACAG GTTTGCTGCT GAGGAGTACG AGGCTCTGGT GACTGAGGAA 
TCTGCCCAGG TTCAGCTCCA GGAAGGCTCA TTTGAAGACT ATGAATCAGA TGAGCCTGTG 
TCTCCCTCTG AACTTGGAAA CAAGGGCAAT AAAATCCTAA CTTCAAGCCT TTCAGAGAAA 
TGTGGAAAGC TCCAGTCAGT GGATGAAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012202.2
CDS	303..3755
Translation	MAATYRLVVSTVNHYSSVVIDRRFEQAIHYCTGTCHTFTHGIDCIVVHHSVCADLLHIPVSQFKDVDLNSVFLSHENGLSSAEDYPHQALTGIPRSKRASTFQNTCNLKDIAGEAISFASGKIKEFSLEKLRNSNHAAYKKGRKVKSDSFNRRSVDLDLLCGHYSNDGTSPSFGLLRSSSVEEKSLSHRNSLDTSLTSMLFQNLSEEDLVTQILEKHKIDHFSSGTDIKMCLDILLKCSEDLKKCTDIIKQCIKKKSGGSINEGSGSDAMSNSETVYMNVMARLAYYLRKLPFEFVQSGSNETVDLTELVNNVPNLQLTPFSPLYGTEQPPKYEDIVHLSALDSRQFQTVELQDDMHNFKQTDTVKTPPNNTNSLCSLEVNDPGTLKPVQLQSPSLTINHLENVSSGNLMETLYIEEESDGKKSLLDKGQKADNGQGQEPLKVNEHKEFSDHDTYLKKCPAPVQNEISKIFEKPMVVRPPKEESELAVPPTELRAQRHFMNPNSQEEIDKLLMDLESFSQKMETSLGEPLAKGKSINSLNSHSHLTGQIHKDLEPKPIVSSATEKVSPSCLTRIIENNGHKIEEEDRALLLRILESIEDFAQELVECKSSRGSLSQEKEMMQILQETLTASSQPTPSVSRSPVGEKPKDSTSVVLIQQTPEVIKIQNKPEKKPATPCPPAATLPSSPQPASPVPCVSDVVNTPLSINIPQFYFPEGLPDTCSNHEQILGRIEAAFKDIEDQKADIHEMGRIAKACGCPLYWKAPMFRAAGGEKTGFVTAQSFMAMWKKLLSDHHDDASRFIWLLAKPSCSHLQQEDFIPLLQDVVDTHPGLTFLKDAPEFHSRYITTVVQRIFYTVNRSWSGKITSTEIRKSNFLQTLALLEEEEDINQITDYFSYEHFYVIYCKFWELDGDHDLYISQADLSRYNDQASSNRIIERIFSGAVTRGKTVQREGRMSYADFVWFLISEEDKRNPTSIEYWFRCMDVDGDGVLSMYELEYFYEEQCERMEAMGIEPLPFHDLLCQMLDLVKPANDGRITLRDLKRCRMAHIFYDTFFNLEKYLDHEQRDPFAVQKDVENEGPEPSDWDRFAAEEYEALVTEESAQVQLQEGSFEDYESDEPVSPSELGNKGNKILTSSLSEKCGKLQSVDEE

Target ORF information:

RefSeq Version	NM_001012202.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012202.2

ORF Insert Sequence:

ATGGCAGCAA CTTACAGACT CGTGGTTAGC ACTGTGAACC ATTATAGCAG TGTAGTGATA 
GACCGGCGTT TTGAACAAGC CATACATTAT TGCACTGGAA CCTGCCATAC CTTCACACAT 
GGAATAGACT GCATTGTAGT GCATCATAGT GTTTGTGCAG ACCTTTTGCA CATCCCTGTG 
TCTCAGTTCA AAGATGTGGA TCTGAACTCT GTGTTTTTGT CTCATGAAAA TGGCCTTTCC 
TCAGCTGAAG ACTATCCCCA TCAGGCCCTC ACAGGAATAC CCAGGTCCAA GAGAGCATCC 
ACATTTCAGA ATACCTGTAA CTTAAAGGAC ATTGCAGGAG AAGCAATCAG CTTTGCCAGT 
GGGAAAATAA AAGAATTTTC CCTTGAAAAA CTCAGAAATT CAAATCATGC AGCTTACAAA 
AAAGGAAGAA AAGTTAAATC TGACTCATTT AATAGGCGAT CAGTTGATTT GGATTTACTC 
TGTGGCCATT ATAGTAACGA TGGGACCTCC CCATCCTTTG GCTTACTCCG GAGTTCCTCA 
GTTGAGGAAA AATCTTTATC TCATAGAAAC TCACTAGATA CAAGCCTAAC TTCCATGCTT 
TTTCAGAACT TGTCTGAAGA AGATCTAGTT ACGCAGATTT TGGAAAAACA TAAAATAGAT 
CATTTTTCTT CTGGGACAGA CATAAAAATG TGCTTGGACA TCTTACTGAA ATGCTCTGAA 
GATTTGAAAA AATGTACAGA CATCATAAAA CAATGCATAA AGAAAAAGTC AGGAGGAAGC 
ATCAACGAAG GAAGTGGCAG TGACGCAATG TCTAACTCTG AAACCGTCTA TATGAATGTA 
ATGGCCAGGT TAGCTTACTA CCTGAGAAAG TTACCATTTG AATTTGTGCA GTCTGGGAGT 
AATGAGACTG TAGATTTGAC AGAGCTTGTC AATAATGTAC CTAACTTACA ACTGACTCCA 
TTCTCCCCAT TATATGGGAC TGAACAGCCC CCTAAATATG AAGATATCGT CCACCTCTCA 
GCCCTTGACT CCAGACAGTT TCAGACTGTT GAATTGCAAG ATGACATGCA TAATTTTAAA 
CAAACAGATA CTGTAAAAAC CCCTCCAAAC AACACAAATT CCTTGTGTAG CTTGGAGGTA 
AACGATCCTG GAACTCTAAA ACCTGTGCAG CTTCAATCAC CGTCATTAAC CATAAACCAT 
TTAGAGAATG TTTCTTCTGG TAACTTAATG GAAACTCTTT ATATTGAAGA AGAGTCAGAT 
GGAAAGAAGT CATTATTAGA TAAAGGACAA AAAGCAGATA ATGGACAAGG TCAGGAACCA 
TTAAAGGTAA ATGAACATAA AGAATTTTCA GACCATGATA CTTATCTTAA AAAATGTCCT 
GCCCCAGTGC AAAATGAAAT CAGTAAGATA TTTGAAAAGC CAATGGTAGT TCGTCCACCT 
AAGGAAGAAT CTGAATTAGC AGTTCCTCCT ACAGAACTCA GAGCCCAGAG GCACTTTATG 
AATCCTAACA GTCAAGAAGA GATCGACAAA TTGTTAATGG ATTTGGAATC ATTCTCACAG 
AAGATGGAGA CCTCTCTAGG AGAACCACTT GCCAAGGGTA AAAGCATCAA TTCTCTAAAT 
AGTCATAGTC ATTTGACTGG TCAGATCCAC AAAGATCTTG AGCCTAAACC TATAGTCTCT 
TCAGCTACGG AGAAAGTCTC ACCTTCTTGT CTAACAAGGA TTATTGAAAA CAACGGACAC 
AAAATAGAGG AAGAGGACCG AGCCCTCTTA CTTCGAATTC TGGAAAGCAT TGAAGACTTT 
GCTCAAGAAC TAGTTGAATG CAAGTCAAGC AGAGGGAGCC TATCACAAGA AAAGGAAATG 
ATGCAAATTC TGCAGGAAAC TTTGACAGCT TCCTCCCAGC CCACTCCATC AGTCAGTAGG 
AGCCCTGTTG GTGAGAAACC CAAAGATAGT ACTTCAGTTG TTTTGATTCA ACAGACACCA 
GAAGTGATCA AGATTCAAAA TAAACCAGAA AAAAAACCTG CAACACCATG TCCACCTGCA 
GCCACCCTTC CAAGTAGTCC CCAGCCTGCC TCCCCTGTTC CTTGTGTGAG CGATGTCGTG 
AATACACCAC TGTCCATAAA CATTCCACAG TTCTACTTTC CCGAAGGACT CCCAGACACC 
TGCAGCAACC ACGAACAGAT CCTAGGCAGA ATTGAAGCTG CTTTCAAGGA TATTGAAGAT 
CAAAAAGCAG ATATTCATGA AATGGGGAGA ATTGCAAAGG CCTGCGGCTG TCCACTCTAT 
TGGAAAGCCC CCATGTTCAG GGCTGCAGGA GGAGAGAAGA CTGGATTTGT GACAGCACAG 
TCATTCATGG CCATGTGGAA AAAGTTGCTA AGTGACCATC ATGATGATGC CTCCAGGTTC 
ATCTGGCTTC TGGCAAAGCC CAGCTGCAGC CATCTCCAAC AAGAGGATTT TATCCCTCTA 
CTTCAGGATG TGGTAGATAC ACACCCTGGC CTCACCTTCC TGAAAGATGC TCCTGAATTC 
CATTCCCGCT ACATCACCAC GGTTGTTCAG AGAATATTCT ACACAGTCAA CAGATCTTGG 
AGTGGAAAAA TTACTTCAAC AGAGATAAGG AAAAGCAACT TTTTGCAAAC TCTGGCCCTT 
TTGGAGGAAG AGGAAGATAT AAACCAAATC ACAGATTACT TCTCTTATGA ACATTTCTAT 
GTCATTTATT GTAAATTCTG GGAACTAGAT GGTGATCATG ACCTATACAT CAGCCAAGCT 
GATCTCTCTC GATACAATGA CCAGGCTTCA TCAAATAGAA TTATTGAAAG AATATTTTCT 
GGAGCAGTAA CAAGAGGGAA AACTGTACAA AGAGAGGGAA GAATGAGCTA TGCAGATTTT 
GTTTGGTTTT TGATTTCTGA GGAAGACAAA CGGAACCCTA CCAGCATTGA GTATTGGTTC 
CGCTGCATGG ATGTGGATGG AGATGGTGTA CTTTCCATGT ATGAACTAGA GTATTTCTAT 
GAAGAGCAGT GTGAACGGAT GGAAGCCATG GGCATCGAGC CCTTGCCGTT TCATGATCTG 
TTGTGCCAGA TGCTAGACTT GGTGAAGCCA GCTAATGATG GCAGAATAAC TCTGCGAGAT 
TTGAAGAGGT GCAGAATGGC TCACATCTTC TACGACACTT TCTTTAACCT GGAGAAGTAC 
TTGGACCATG AACAAAGAGA TCCTTTTGCA GTCCAGAAGG ATGTTGAAAA TGAGGGCCCC 
GAGCCCTCTG ACTGGGACAG GTTTGCTGCT GAGGAGTACG AGGCTCTGGT GACTGAGGAA 
TCTGCCCAGG TTCAGCTCCA GGAAGGCTCA TTTGAAGACT ATGAATCAGA TGAGCCTGTG 
TCTCCCTCTG AACTTGGAAA CAAGGGCAAT AAAATCCTAA CTTCAAGCCT TTCAGAGAAA 
TGTGGAAAGC TCCAGTCAGT GGATGAAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp2r3a ( XM_006243633.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243695.1 Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), transcript variant X1, mRNA.

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CloneID	ORa02681
Clone ID Related Accession (Same CDS sequence)	NM_001012202.2 , XM_006243633.3
Accession Version	XM_006243633.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243633.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA CTTACAGACT CGTGGTTAGC ACTGTGAACC ATTATAGCAG TGTAGTGATA 
GACCGGCGTT TTGAACAAGC CATACATTAT TGCACTGGAA CCTGCCATAC CTTCACACAT 
GGAATAGACT GCATTGTAGT GCATCATAGT GTTTGTGCAG ACCTTTTGCA CATCCCTGTG 
TCTCAGTTCA AAGATGTGGA TCTGAACTCT GTGTTTTTGT CTCATGAAAA TGGCCTTTCC 
TCAGCTGAAG ACTATCCCCA TCAGGCCCTC ACAGGAATAC CCAGGTCCAA GAGAGCATCC 
ACATTTCAGA ATACCTGTAA CTTAAAGGAC ATTGCAGGAG AAGCAATCAG CTTTGCCAGT 
GGGAAAATAA AAGAATTTTC CCTTGAAAAA CTCAGAAATT CAAATCATGC AGCTTACAAA 
AAAGGAAGAA AAGTTAAATC TGACTCATTT AATAGGCGAT CAGTTGATTT GGATTTACTC 
TGTGGCCATT ATAGTAACGA TGGGACCTCC CCATCCTTTG GCTTACTCCG GAGTTCCTCA 
GTTGAGGAAA AATCTTTATC TCATAGAAAC TCACTAGATA CAAGCCTAAC TTCCATGCTT 
TTTCAGAACT TGTCTGAAGA AGATCTAGTT ACGCAGATTT TGGAAAAACA TAAAATAGAT 
CATTTTTCTT CTGGGACAGA CATAAAAATG TGCTTGGACA TCTTACTGAA ATGCTCTGAA 
GATTTGAAAA AATGTACAGA CATCATAAAA CAATGCATAA AGAAAAAGTC AGGAGGAAGC 
ATCAACGAAG GAAGTGGCAG TGACGCAATG TCTAACTCTG AAACCGTCTA TATGAATGTA 
ATGGCCAGGT TAGCTTACTA CCTGAGAAAG TTACCATTTG AATTTGTGCA GTCTGGGAGT 
AATGAGACTG TAGATTTGAC AGAGCTTGTC AATAATGTAC CTAACTTACA ACTGACTCCA 
TTCTCCCCAT TATATGGGAC TGAACAGCCC CCTAAATATG AAGATATCGT CCACCTCTCA 
GCCCTTGACT CCAGACAGTT TCAGACTGTT GAATTGCAAG ATGACATGCA TAATTTTAAA 
CAAACAGATA CTGTAAAAAC CCCTCCAAAC AACACAAATT CCTTGTGTAG CTTGGAGGTA 
AACGATCCTG GAACTCTAAA ACCTGTGCAG CTTCAATCAC CGTCATTAAC CATAAACCAT 
TTAGAGAATG TTTCTTCTGG TAACTTAATG GAAACTCTTT ATATTGAAGA AGAGTCAGAT 
GGAAAGAAGT CATTATTAGA TAAAGGACAA AAAGCAGATA ATGGACAAGG TCAGGAACCA 
TTAAAGGTAA ATGAACATAA AGAATTTTCA GACCATGATA CTTATCTTAA AAAATGTCCT 
GCCCCAGTGC AAAATGAAAT CAGTAAGATA TTTGAAAAGC CAATGGTAGT TCGTCCACCT 
AAGGAAGAAT CTGAATTAGC AGTTCCTCCT ACAGAACTCA GAGCCCAGAG GCACTTTATG 
AATCCTAACA GTCAAGAAGA GATCGACAAA TTGTTAATGG ATTTGGAATC ATTCTCACAG 
AAGATGGAGA CCTCTCTAGG AGAACCACTT GCCAAGGGTA AAAGCATCAA TTCTCTAAAT 
AGTCATAGTC ATTTGACTGG TCAGATCCAC AAAGATCTTG AGCCTAAACC TATAGTCTCT 
TCAGCTACGG AGAAAGTCTC ACCTTCTTGT CTAACAAGGA TTATTGAAAA CAACGGACAC 
AAAATAGAGG AAGAGGACCG AGCCCTCTTA CTTCGAATTC TGGAAAGCAT TGAAGACTTT 
GCTCAAGAAC TAGTTGAATG CAAGTCAAGC AGAGGGAGCC TATCACAAGA AAAGGAAATG 
ATGCAAATTC TGCAGGAAAC TTTGACAGCT TCCTCCCAGC CCACTCCATC AGTCAGTAGG 
AGCCCTGTTG GTGAGAAACC CAAAGATAGT ACTTCAGTTG TTTTGATTCA ACAGACACCA 
GAAGTGATCA AGATTCAAAA TAAACCAGAA AAAAAACCTG CAACACCATG TCCACCTGCA 
GCCACCCTTC CAAGTAGTCC CCAGCCTGCC TCCCCTGTTC CTTGTGTGAG CGATGTCGTG 
AATACACCAC TGTCCATAAA CATTCCACAG TTCTACTTTC CCGAAGGACT CCCAGACACC 
TGCAGCAACC ACGAACAGAT CCTAGGCAGA ATTGAAGCTG CTTTCAAGGA TATTGAAGAT 
CAAAAAGCAG ATATTCATGA AATGGGGAGA ATTGCAAAGG CCTGCGGCTG TCCACTCTAT 
TGGAAAGCCC CCATGTTCAG GGCTGCAGGA GGAGAGAAGA CTGGATTTGT GACAGCACAG 
TCATTCATGG CCATGTGGAA AAAGTTGCTA AGTGACCATC ATGATGATGC CTCCAGGTTC 
ATCTGGCTTC TGGCAAAGCC CAGCTGCAGC CATCTCCAAC AAGAGGATTT TATCCCTCTA 
CTTCAGGATG TGGTAGATAC ACACCCTGGC CTCACCTTCC TGAAAGATGC TCCTGAATTC 
CATTCCCGCT ACATCACCAC GGTTGTTCAG AGAATATTCT ACACAGTCAA CAGATCTTGG 
AGTGGAAAAA TTACTTCAAC AGAGATAAGG AAAAGCAACT TTTTGCAAAC TCTGGCCCTT 
TTGGAGGAAG AGGAAGATAT AAACCAAATC ACAGATTACT TCTCTTATGA ACATTTCTAT 
GTCATTTATT GTAAATTCTG GGAACTAGAT GGTGATCATG ACCTATACAT CAGCCAAGCT 
GATCTCTCTC GATACAATGA CCAGGCTTCA TCAAATAGAA TTATTGAAAG AATATTTTCT 
GGAGCAGTAA CAAGAGGGAA AACTGTACAA AGAGAGGGAA GAATGAGCTA TGCAGATTTT 
GTTTGGTTTT TGATTTCTGA GGAAGACAAA CGGAACCCTA CCAGCATTGA GTATTGGTTC 
CGCTGCATGG ATGTGGATGG AGATGGTGTA CTTTCCATGT ATGAACTAGA GTATTTCTAT 
GAAGAGCAGT GTGAACGGAT GGAAGCCATG GGCATCGAGC CCTTGCCGTT TCATGATCTG 
TTGTGCCAGA TGCTAGACTT GGTGAAGCCA GCTAATGATG GCAGAATAAC TCTGCGAGAT 
TTGAAGAGGT GCAGAATGGC TCACATCTTC TACGACACTT TCTTTAACCT GGAGAAGTAC 
TTGGACCATG AACAAAGAGA TCCTTTTGCA GTCCAGAAGG ATGTTGAAAA TGAGGGCCCC 
GAGCCCTCTG ACTGGGACAG GTTTGCTGCT GAGGAGTACG AGGCTCTGGT GACTGAGGAA 
TCTGCCCAGG TTCAGCTCCA GGAAGGCTCA TTTGAAGACT ATGAATCAGA TGAGCCTGTG 
TCTCCCTCTG AACTTGGAAA CAAGGGCAAT AAAATCCTAA CTTCAAGCCT TTCAGAGAAA 
TGTGGAAAGC TCCAGTCAGT GGATGAAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243695.1
CDS	607..4059
Translation	MAATYRLVVSTVNHYSSVVIDRRFEQAIHYCTGTCHTFTHGIDCIVVHHSVCADLLHIPVSQFKDVDLNSVFLSHENGLSSAEDYPHQALTGIPRSKRASTFQNTCNLKDIAGEAISFASGKIKEFSLEKLRNSNHAAYKKGRKVKSDSFNRRSVDLDLLCGHYSNDGTSPSFGLLRSSSVEEKSLSHRNSLDTSLTSMLFQNLSEEDLVTQILEKHKIDHFSSGTDIKMCLDILLKCSEDLKKCTDIIKQCIKKKSGGSINEGSGSDAMSNSETVYMNVMARLAYYLRKLPFEFVQSGSNETVDLTELVNNVPNLQLTPFSPLYGTEQPPKYEDIVHLSALDSRQFQTVELQDDMHNFKQTDTVKTPPNNTNSLCSLEVNDPGTLKPVQLQSPSLTINHLENVSSGNLMETLYIEEESDGKKSLLDKGQKADNGQGQEPLKVNEHKEFSDHDTYLKKCPAPVQNEISKIFEKPMVVRPPKEESELAVPPTELRAQRHFMNPNSQEEIDKLLMDLESFSQKMETSLGEPLAKGKSINSLNSHSHLTGQIHKDLEPKPIVSSATEKVSPSCLTRIIENNGHKIEEEDRALLLRILESIEDFAQELVECKSSRGSLSQEKEMMQILQETLTASSQPTPSVSRSPVGEKPKDSTSVVLIQQTPEVIKIQNKPEKKPATPCPPAATLPSSPQPASPVPCVSDVVNTPLSINIPQFYFPEGLPDTCSNHEQILGRIEAAFKDIEDQKADIHEMGRIAKACGCPLYWKAPMFRAAGGEKTGFVTAQSFMAMWKKLLSDHHDDASRFIWLLAKPSCSHLQQEDFIPLLQDVVDTHPGLTFLKDAPEFHSRYITTVVQRIFYTVNRSWSGKITSTEIRKSNFLQTLALLEEEEDINQITDYFSYEHFYVIYCKFWELDGDHDLYISQADLSRYNDQASSNRIIERIFSGAVTRGKTVQREGRMSYADFVWFLISEEDKRNPTSIEYWFRCMDVDGDGVLSMYELEYFYEEQCERMEAMGIEPLPFHDLLCQMLDLVKPANDGRITLRDLKRCRMAHIFYDTFFNLEKYLDHEQRDPFAVQKDVENEGPEPSDWDRFAAEEYEALVTEESAQVQLQEGSFEDYESDEPVSPSELGNKGNKILTSSLSEKCGKLQSVDEE

Target ORF information:

RefSeq Version	XM_006243633.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 2, regulatory subunit B'', alpha (Ppp2r3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243633.3

ORF Insert Sequence:

ATGGCAGCAA CTTACAGACT CGTGGTTAGC ACTGTGAACC ATTATAGCAG TGTAGTGATA 
GACCGGCGTT TTGAACAAGC CATACATTAT TGCACTGGAA CCTGCCATAC CTTCACACAT 
GGAATAGACT GCATTGTAGT GCATCATAGT GTTTGTGCAG ACCTTTTGCA CATCCCTGTG 
TCTCAGTTCA AAGATGTGGA TCTGAACTCT GTGTTTTTGT CTCATGAAAA TGGCCTTTCC 
TCAGCTGAAG ACTATCCCCA TCAGGCCCTC ACAGGAATAC CCAGGTCCAA GAGAGCATCC 
ACATTTCAGA ATACCTGTAA CTTAAAGGAC ATTGCAGGAG AAGCAATCAG CTTTGCCAGT 
GGGAAAATAA AAGAATTTTC CCTTGAAAAA CTCAGAAATT CAAATCATGC AGCTTACAAA 
AAAGGAAGAA AAGTTAAATC TGACTCATTT AATAGGCGAT CAGTTGATTT GGATTTACTC 
TGTGGCCATT ATAGTAACGA TGGGACCTCC CCATCCTTTG GCTTACTCCG GAGTTCCTCA 
GTTGAGGAAA AATCTTTATC TCATAGAAAC TCACTAGATA CAAGCCTAAC TTCCATGCTT 
TTTCAGAACT TGTCTGAAGA AGATCTAGTT ACGCAGATTT TGGAAAAACA TAAAATAGAT 
CATTTTTCTT CTGGGACAGA CATAAAAATG TGCTTGGACA TCTTACTGAA ATGCTCTGAA 
GATTTGAAAA AATGTACAGA CATCATAAAA CAATGCATAA AGAAAAAGTC AGGAGGAAGC 
ATCAACGAAG GAAGTGGCAG TGACGCAATG TCTAACTCTG AAACCGTCTA TATGAATGTA 
ATGGCCAGGT TAGCTTACTA CCTGAGAAAG TTACCATTTG AATTTGTGCA GTCTGGGAGT 
AATGAGACTG TAGATTTGAC AGAGCTTGTC AATAATGTAC CTAACTTACA ACTGACTCCA 
TTCTCCCCAT TATATGGGAC TGAACAGCCC CCTAAATATG AAGATATCGT CCACCTCTCA 
GCCCTTGACT CCAGACAGTT TCAGACTGTT GAATTGCAAG ATGACATGCA TAATTTTAAA 
CAAACAGATA CTGTAAAAAC CCCTCCAAAC AACACAAATT CCTTGTGTAG CTTGGAGGTA 
AACGATCCTG GAACTCTAAA ACCTGTGCAG CTTCAATCAC CGTCATTAAC CATAAACCAT 
TTAGAGAATG TTTCTTCTGG TAACTTAATG GAAACTCTTT ATATTGAAGA AGAGTCAGAT 
GGAAAGAAGT CATTATTAGA TAAAGGACAA AAAGCAGATA ATGGACAAGG TCAGGAACCA 
TTAAAGGTAA ATGAACATAA AGAATTTTCA GACCATGATA CTTATCTTAA AAAATGTCCT 
GCCCCAGTGC AAAATGAAAT CAGTAAGATA TTTGAAAAGC CAATGGTAGT TCGTCCACCT 
AAGGAAGAAT CTGAATTAGC AGTTCCTCCT ACAGAACTCA GAGCCCAGAG GCACTTTATG 
AATCCTAACA GTCAAGAAGA GATCGACAAA TTGTTAATGG ATTTGGAATC ATTCTCACAG 
AAGATGGAGA CCTCTCTAGG AGAACCACTT GCCAAGGGTA AAAGCATCAA TTCTCTAAAT 
AGTCATAGTC ATTTGACTGG TCAGATCCAC AAAGATCTTG AGCCTAAACC TATAGTCTCT 
TCAGCTACGG AGAAAGTCTC ACCTTCTTGT CTAACAAGGA TTATTGAAAA CAACGGACAC 
AAAATAGAGG AAGAGGACCG AGCCCTCTTA CTTCGAATTC TGGAAAGCAT TGAAGACTTT 
GCTCAAGAAC TAGTTGAATG CAAGTCAAGC AGAGGGAGCC TATCACAAGA AAAGGAAATG 
ATGCAAATTC TGCAGGAAAC TTTGACAGCT TCCTCCCAGC CCACTCCATC AGTCAGTAGG 
AGCCCTGTTG GTGAGAAACC CAAAGATAGT ACTTCAGTTG TTTTGATTCA ACAGACACCA 
GAAGTGATCA AGATTCAAAA TAAACCAGAA AAAAAACCTG CAACACCATG TCCACCTGCA 
GCCACCCTTC CAAGTAGTCC CCAGCCTGCC TCCCCTGTTC CTTGTGTGAG CGATGTCGTG 
AATACACCAC TGTCCATAAA CATTCCACAG TTCTACTTTC CCGAAGGACT CCCAGACACC 
TGCAGCAACC ACGAACAGAT CCTAGGCAGA ATTGAAGCTG CTTTCAAGGA TATTGAAGAT 
CAAAAAGCAG ATATTCATGA AATGGGGAGA ATTGCAAAGG CCTGCGGCTG TCCACTCTAT 
TGGAAAGCCC CCATGTTCAG GGCTGCAGGA GGAGAGAAGA CTGGATTTGT GACAGCACAG 
TCATTCATGG CCATGTGGAA AAAGTTGCTA AGTGACCATC ATGATGATGC CTCCAGGTTC 
ATCTGGCTTC TGGCAAAGCC CAGCTGCAGC CATCTCCAAC AAGAGGATTT TATCCCTCTA 
CTTCAGGATG TGGTAGATAC ACACCCTGGC CTCACCTTCC TGAAAGATGC TCCTGAATTC 
CATTCCCGCT ACATCACCAC GGTTGTTCAG AGAATATTCT ACACAGTCAA CAGATCTTGG 
AGTGGAAAAA TTACTTCAAC AGAGATAAGG AAAAGCAACT TTTTGCAAAC TCTGGCCCTT 
TTGGAGGAAG AGGAAGATAT AAACCAAATC ACAGATTACT TCTCTTATGA ACATTTCTAT 
GTCATTTATT GTAAATTCTG GGAACTAGAT GGTGATCATG ACCTATACAT CAGCCAAGCT 
GATCTCTCTC GATACAATGA CCAGGCTTCA TCAAATAGAA TTATTGAAAG AATATTTTCT 
GGAGCAGTAA CAAGAGGGAA AACTGTACAA AGAGAGGGAA GAATGAGCTA TGCAGATTTT 
GTTTGGTTTT TGATTTCTGA GGAAGACAAA CGGAACCCTA CCAGCATTGA GTATTGGTTC 
CGCTGCATGG ATGTGGATGG AGATGGTGTA CTTTCCATGT ATGAACTAGA GTATTTCTAT 
GAAGAGCAGT GTGAACGGAT GGAAGCCATG GGCATCGAGC CCTTGCCGTT TCATGATCTG 
TTGTGCCAGA TGCTAGACTT GGTGAAGCCA GCTAATGATG GCAGAATAAC TCTGCGAGAT 
TTGAAGAGGT GCAGAATGGC TCACATCTTC TACGACACTT TCTTTAACCT GGAGAAGTAC 
TTGGACCATG AACAAAGAGA TCCTTTTGCA GTCCAGAAGG ATGTTGAAAA TGAGGGCCCC 
GAGCCCTCTG ACTGGGACAG GTTTGCTGCT GAGGAGTACG AGGCTCTGGT GACTGAGGAA 
TCTGCCCAGG TTCAGCTCCA GGAAGGCTCA TTTGAAGACT ATGAATCAGA TGAGCCTGTG 
TCTCCCTCTG AACTTGGAAA CAAGGGCAAT AAAATCCTAA CTTCAAGCCT TTCAGAGAAA 
TGTGGAAAGC TCCAGTCAGT GGATGAAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DARPP-32, Jack of All Trades… Master of Which?
Frontiers in behavioral neuroscience556(2011)
Yger M,Girault JA

Structure of protein phosphatase 2A core enzyme bound to tumor-inducing toxins.
Cell127(2)341-53(2006 Oct)
Xing Y,Xu Y,Chen Y,Jeffrey PD,Chao Y,Lin Z,Li Z,Strack S,Stock JB,Shi Y

PR130 is a modulator of the Wnt-signaling cascade that counters repression of the antagonist Naked cuticle.
Proceedings of the National Academy of Sciences of the United States of America103(14)5397-402(2006 Apr)
Creyghton MP,Roël G,Eichhorn PJ,Vredeveld LC,Destrée O,Bernards R

PR72, a novel regulator of Wnt signaling required for Naked cuticle function.
Genes & development19(3)376-86(2005 Feb)
Creyghton MP,Roël G,Eichhorn PJ,Hijmans EM,Maurer I,Destrée O,Bernards R

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Ppp2r3a cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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