Lig4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lig4
Entrez Gene ID	290907
Full Name	DNA ligase 4
General protein information	Preferred Names DNA ligase 4 Names DNA ligase 4 DNA ligase (ATP) 4 ligase IV, DNA, ATP-dependent
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006253477.3	XP_006253539.1	DNA ligase 4 isoform X1
XM_017600050.1	XP_017455539.1	DNA ligase 4 isoform X1
NM_001106095.1	NP_001099565.1	DNA ligase 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Lig4	NP_001099565.1
Drosophila melanogaster (fruit fly)	Lig4	NP_572907.1
Magnaporthe oryzae (rice blast fungus)	MGG_12899	XP_003712808.1
Bos taurus (cattle)	LIG4	NP_001178055.1
Saccharomyces cerevisiae (baker's yeast)	DNL4	NP_014647.1
Eremothecium gossypii	AGOS_ACR008W	NP_983411.1
Kluyveromyces lactis	KLLA0D01089g	XP_453119.1
Neurospora crassa	NCU06264	XP_962868.1
Gallus gallus (chicken)	LIG4	NP_001025987.1
Mus musculus (house mouse)	Lig4	NP_795927.2
Danio rerio (zebrafish)	lig4	NP_001096593.1
Arabidopsis thaliana (thale cress)	ATLIG4	NP_568851.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000623	XP_310455.4
Homo sapiens (human)	LIG4	NP_001091738.1
Pan troglodytes (chimpanzee)	LIG4	XP_003314254.1
Canis lupus familiaris (dog)	LIG4	XP_005634152.1
Xenopus tropicalis (tropical clawed frog)	lig4	NP_001016981.1
Schizosaccharomyces pombe (fission yeast)	lig4	NP_587888.2

Gene Ontology
Bibliography

Related articles in PubMed

LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during late mitosis.
Hong Y, Sonneville R, Wang B, Scheidt V, Meier B, Woglar A, Demetriou S, Labib K, Jantsch V, Gartner A
Nature communications9(1)728(2018 02)

Ligase-4 Deficiency Causes Distinctive Immune Abnormalities in Asymptomatic Individuals.
Felgentreff K, Baxi SN, Lee YN, Dobbs K, Henderson LA, Csomos K, Tsitsikov EN, Armanios M, Walter JE, Notarangelo LD
Journal of clinical immunology36(4)341-53(2016 05)

XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair.
Craxton A, Somers J, Munnur D, Jukes-Jones R, Cain K, Malewicz M
Cell death and differentiation22(6)890-7(2015 Jun)

DNA ligase III and DNA ligase IV carry out genetically distinct forms of end joining in human somatic cells.
Oh S, Harvey A, Zimbric J, Wang Y, Nguyen T, Jackson PJ, Hendrickson EA
DNA repair2197-110(2014 Sep)

Altered regulation of DNA ligase IV activity by aberrant promoter DNA methylation and gene amplification in colorectal cancer.
Kuhmann C, Li C, Kloor M, Salou M, Weigel C, Schmidt CR, Ng LW, Tsui WW, Leung SY, Yuen ST, Becker N, Weichenhan D, Plass C, Schmezer P, Chan TL, Popanda O
Human molecular genetics23(8)2043-54(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lig4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lig4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00169	NM_001106095.1 Latest version!	Rattus norvegicus DNA ligase 4 (Lig4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa00169	XM_006253477.3 Latest version!	Rattus norvegicus DNA ligase 4 (Lig4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa00169	XM_017600050.1 Latest version!	Rattus norvegicus DNA ligase 4 (Lig4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lig4 ( NM_001106095.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099565.1 Rattus norvegicus DNA ligase 4 (Lig4), mRNA.

Price & Availability↑

CloneID	ORa00169
Clone ID Related Accession (Same CDS sequence)	XM_006253477.3 , XM_017600050.1 , NM_001106095.1
Accession Version	NM_001106095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473970.2. On or before Oct 4, 2007 this sequence version replaced XM_225045.4, XM_001067682.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: CB734948.1, CO395037.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTCCT CACAAGCCTC ACGAACTGTC GCAGCTCATG TCCCCTTTGC AGACTTGTGC 
TCCACCTTAG AACGAATACA GAAAGGTAAA GACCGTGCAG AAAAAATCAG GCACTTCAAG 
GAGTTTCTGG ATTCTTGGAG AAAATTTCAT GATGCCCTTC ATAAGAACAA GAAGGATGTT 
CCGGACTCTT TTTACCCAGC AATGAGACTT ATTCTCCCTC AGCTAGAGAG AGAGAGGATG 
GCTTACGGAA TCAAAGAAAC CATGCTGGCT AAGCTTTACA TCGAATTGCT CAATTTACCA 
AGAGAAGGCA AGGATGCCCA GAAGCTCCTG AATTACCGAA CCCCCAGTGG AGCTCGCTCA 
GATGCTGGGG ACTTTGCCAC GATTGCATAC TTTGTTTTGA AGCCAAGGTG CTTACAGAAA 
GGAAGCTTAA CCATACAGAA GGTGAATGAA CTCTTAGACT TAGTTGCCAG CAATAACTCT 
GGCAAAAGAA AAGATCTAGT GAAAAAGAGC CTTCTTCAGT TAATAACCCA GTGTTCAGCA 
CTCGAGCAAA AGTGGCTGAT TCGCATGATT ATTAAGGACT TAAAGCTTGG TGTCAGTCAG 
CACACGATAT TGTCCATCTT CCACAATGAT GCAGTCGAGT TGCACAATGT CACCACAGAT 
CTGGAAAAGG TCTGTAGGCA GCTACATGAC CCCTCCGTAG GGCTCAGCGA CATCTCCATC 
ACTTTGTTCT CTGCCTTTAG GCCAATGCTA GCTGCTGTAG CAGATGTGGA GCGTGTGGAG 
AAGGACATGA AGCAGCAGAG TTTCTACATC GAAACTAAGC TCGATGGCGA GCGCATGCAG 
ATGCACAAGG ATGGCGCCCT GTACCGGTAC TTCTCCAGAA ATGGTTACAA CTACACCGAT 
CAGTTCGGCG AATCTCCACA GGAAGGCTCT CTCACCCCGT TTATTCACAA CGCATTCAAG 
ACAGATGTGC AAGTGTGCAT CCTTGATGGC GAGATGATGG CCTACAACCC AACGACACAG 
ACTTTCATGC AGAAGGGGGT CAAGTTTGAT ATCAAAAGGA TGGTCGAAGA TTCTGACCTA 
CAGACATGCT ACTGTGTTTT CGATGTGCTG ATGGTTAATA ATAAGAAGCT AGGGCGTGAG 
ACTCTCAGGA AGAGGTATGA TATCCTTAGT AGCACTTTCA CACCCATACA AGGTCGAATA 
GAGATAGTGC AGAAAACTCA AGCTCAGACA AAGCAGGAAG TAGTGGATGC ATTAAATGAT 
GCCATAGACA AGAGAGAAGA GGGGATCATG GTTAAACACC CTCTGTCCAT TTACAAGCCA 
GACAAAAGAG GTGAAGGGTG GCTAAAGATT AAACCAGAGT ATGTCACTGG ATTGATGGAT 
GAATTAGACC TCTTAATTGT TGGGGGCTAC TGGGGTAAAG GTTCTCGAGG TGGCATGATG 
TCTCACTTTC TGTGTGCAGT GGCAGAGACA CCACCTCCTG GTGACAAGCC ATCTGTGTTC 
CACACTCTGT GCCGTGTTGG GTCGGGTTAC ACGATGAAAG AACTCTATGA CCTGGGCTTG 
AAATTGGCAA AATACTGGAA GCCTTTCCAA AGAAAATCTC CACCAAGCTG TATTTTGTGT 
GGCACAGAGA AGCCAGAAGT GTACATTGAA CCCCAGAACT CTGTCATCGT TCAGATCAAG 
GCGGCAGAGA TCGTCCCCAG TGACATGTAC AAGACTGGCT CTACCTTGCG CTTCCCACGC 
ATCGAGAAGA TCAGAGATGA CAAAGAGTGG CATGACTGTA TGACGCTGGA TGACCTGGAG 
CAGCTGAGGG GGAAAGCATC TGGGAAGCTT GCCACAAAAC ACCTTCACAT AGGTGACGAT 
GACGAACCCA GAGAAAAAAG GCGGAAACCC ATCTCTAAGA CGAAGAAAAC CATTGGAATC 
ATTGAACACC TAAAAGCGCC TAACCTTTCT AACATAAACA AAGTTTCTAA TGTATTTGAA 
GATGTTGAGT TTTGTGTCAT GAGTGGATTA GATGGTTATC CAAAGCCTGA CCTAGAGAAC 
AAAATTGCAG AATTTGGTGG TTATATAGTC CAGAATCCAG GCCCAGATAC ATACTGTGTG 
ATTGCAGGTT CTGAGAACAT TAGGGTCAGA AACATCATCT CTTCAGATAG GCATGATGTT 
GTCAAGCCCG AGTGGCTTTT AGAGTGTTTT AAAACAAAAA CATGTGTGCC ATGGCAACCC 
CGCTTTATGA TTCACATGTG CCCATCAACA AAGCAGCATT TTGCCCGTGA ATATGACTGC 
TATGGTGATA GCTATTTCGT TGATGCAGAT TTGGATCAAT TGAAAGAAGT GTTTTTAAGA 
ATTAAACCCA GTGAGCAGCA GACTCCTGAA GAAATGGCCC CTGTGATTGC TGACCTAGAA 
TGTCGTTATT CCTGGGATCA CTCTCCTCTC AGTATGTTTC GACACTACAC CGTTTATTTG 
GACTTGTATG CTGTTATTAA TGACTTAAGT TCCAAAATCG AAGCCACAAG GTTAGGTGTT 
ACAGCACTTG AGCTGCGGTT TCGTGGAGCA AAGGTGGTTT CCCGCTTATC TGAAGGGGTG 
TCTCACGTAA TCATCGGGGA AGATCAGAGA CGAGTCAGTG ACTTTAAAAC GTTCAGAAGA 
ACGCTTAAGA AAAAGTTTAA AATCCTGCAG GAACGTTGGG TGATCGATTC AGTAGACAAG 
TGTGAACTGC AGGAAGAAAA CCTGTATTTG CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099565.1
CDS	283..3018
Translation	MASSQASRTVAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKNKKDVPDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGARSDAGDFATIAYFVLKPRCLQKGSLTIQKVNELLDLVASNNSGKRKDLVKKSLLQLITQCSALEQKWLIRMIIKDLKLGVSQHTILSIFHNDAVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFRPMLAAVADVERVEKDMKQQSFYIETKLDGERMQMHKDGALYRYFSRNGYNYTDQFGESPQEGSLTPFIHNAFKTDVQVCILDGEMMAYNPTTQTFMQKGVKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGRETLRKRYDILSSTFTPIQGRIEIVQKTQAQTKQEVVDALNDAIDKREEGIMVKHPLSIYKPDKRGEGWLKIKPEYVTGLMDELDLLIVGGYWGKGSRGGMMSHFLCAVAETPPPGDKPSVFHTLCRVGSGYTMKELYDLGLKLAKYWKPFQRKSPPSCILCGTEKPEVYIEPQNSVIVQIKAAEIVPSDMYKTGSTLRFPRIEKIRDDKEWHDCMTLDDLEQLRGKASGKLATKHLHIGDDDEPREKRRKPISKTKKTIGIIEHLKAPNLSNINKVSNVFEDVEFCVMSGLDGYPKPDLENKIAEFGGYIVQNPGPDTYCVIAGSENIRVRNIISSDRHDVVKPEWLLECFKTKTCVPWQPRFMIHMCPSTKQHFAREYDCYGDSYFVDADLDQLKEVFLRIKPSEQQTPEEMAPVIADLECRYSWDHSPLSMFRHYTVYLDLYAVINDLSSKIEATRLGVTALELRFRGAKVVSRLSEGVSHVIIGEDQRRVSDFKTFRRTLKKKFKILQERWVIDSVDKCELQEENLYLL

Target ORF information:

RefSeq Version	NM_001106095.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 4 (Lig4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106095.1

ORF Insert Sequence:

ATGGCTTCCT CACAAGCCTC ACGAACTGTC GCAGCTCATG TCCCCTTTGC AGACTTGTGC 
TCCACCTTAG AACGAATACA GAAAGGTAAA GACCGTGCAG AAAAAATCAG GCACTTCAAG 
GAGTTTCTGG ATTCTTGGAG AAAATTTCAT GATGCCCTTC ATAAGAACAA GAAGGATGTT 
CCGGACTCTT TTTACCCAGC AATGAGACTT ATTCTCCCTC AGCTAGAGAG AGAGAGGATG 
GCTTACGGAA TCAAAGAAAC CATGCTGGCT AAGCTTTACA TCGAATTGCT CAATTTACCA 
AGAGAAGGCA AGGATGCCCA GAAGCTCCTG AATTACCGAA CCCCCAGTGG AGCTCGCTCA 
GATGCTGGGG ACTTTGCCAC GATTGCATAC TTTGTTTTGA AGCCAAGGTG CTTACAGAAA 
GGAAGCTTAA CCATACAGAA GGTGAATGAA CTCTTAGACT TAGTTGCCAG CAATAACTCT 
GGCAAAAGAA AAGATCTAGT GAAAAAGAGC CTTCTTCAGT TAATAACCCA GTGTTCAGCA 
CTCGAGCAAA AGTGGCTGAT TCGCATGATT ATTAAGGACT TAAAGCTTGG TGTCAGTCAG 
CACACGATAT TGTCCATCTT CCACAATGAT GCAGTCGAGT TGCACAATGT CACCACAGAT 
CTGGAAAAGG TCTGTAGGCA GCTACATGAC CCCTCCGTAG GGCTCAGCGA CATCTCCATC 
ACTTTGTTCT CTGCCTTTAG GCCAATGCTA GCTGCTGTAG CAGATGTGGA GCGTGTGGAG 
AAGGACATGA AGCAGCAGAG TTTCTACATC GAAACTAAGC TCGATGGCGA GCGCATGCAG 
ATGCACAAGG ATGGCGCCCT GTACCGGTAC TTCTCCAGAA ATGGTTACAA CTACACCGAT 
CAGTTCGGCG AATCTCCACA GGAAGGCTCT CTCACCCCGT TTATTCACAA CGCATTCAAG 
ACAGATGTGC AAGTGTGCAT CCTTGATGGC GAGATGATGG CCTACAACCC AACGACACAG 
ACTTTCATGC AGAAGGGGGT CAAGTTTGAT ATCAAAAGGA TGGTCGAAGA TTCTGACCTA 
CAGACATGCT ACTGTGTTTT CGATGTGCTG ATGGTTAATA ATAAGAAGCT AGGGCGTGAG 
ACTCTCAGGA AGAGGTATGA TATCCTTAGT AGCACTTTCA CACCCATACA AGGTCGAATA 
GAGATAGTGC AGAAAACTCA AGCTCAGACA AAGCAGGAAG TAGTGGATGC ATTAAATGAT 
GCCATAGACA AGAGAGAAGA GGGGATCATG GTTAAACACC CTCTGTCCAT TTACAAGCCA 
GACAAAAGAG GTGAAGGGTG GCTAAAGATT AAACCAGAGT ATGTCACTGG ATTGATGGAT 
GAATTAGACC TCTTAATTGT TGGGGGCTAC TGGGGTAAAG GTTCTCGAGG TGGCATGATG 
TCTCACTTTC TGTGTGCAGT GGCAGAGACA CCACCTCCTG GTGACAAGCC ATCTGTGTTC 
CACACTCTGT GCCGTGTTGG GTCGGGTTAC ACGATGAAAG AACTCTATGA CCTGGGCTTG 
AAATTGGCAA AATACTGGAA GCCTTTCCAA AGAAAATCTC CACCAAGCTG TATTTTGTGT 
GGCACAGAGA AGCCAGAAGT GTACATTGAA CCCCAGAACT CTGTCATCGT TCAGATCAAG 
GCGGCAGAGA TCGTCCCCAG TGACATGTAC AAGACTGGCT CTACCTTGCG CTTCCCACGC 
ATCGAGAAGA TCAGAGATGA CAAAGAGTGG CATGACTGTA TGACGCTGGA TGACCTGGAG 
CAGCTGAGGG GGAAAGCATC TGGGAAGCTT GCCACAAAAC ACCTTCACAT AGGTGACGAT 
GACGAACCCA GAGAAAAAAG GCGGAAACCC ATCTCTAAGA CGAAGAAAAC CATTGGAATC 
ATTGAACACC TAAAAGCGCC TAACCTTTCT AACATAAACA AAGTTTCTAA TGTATTTGAA 
GATGTTGAGT TTTGTGTCAT GAGTGGATTA GATGGTTATC CAAAGCCTGA CCTAGAGAAC 
AAAATTGCAG AATTTGGTGG TTATATAGTC CAGAATCCAG GCCCAGATAC ATACTGTGTG 
ATTGCAGGTT CTGAGAACAT TAGGGTCAGA AACATCATCT CTTCAGATAG GCATGATGTT 
GTCAAGCCCG AGTGGCTTTT AGAGTGTTTT AAAACAAAAA CATGTGTGCC ATGGCAACCC 
CGCTTTATGA TTCACATGTG CCCATCAACA AAGCAGCATT TTGCCCGTGA ATATGACTGC 
TATGGTGATA GCTATTTCGT TGATGCAGAT TTGGATCAAT TGAAAGAAGT GTTTTTAAGA 
ATTAAACCCA GTGAGCAGCA GACTCCTGAA GAAATGGCCC CTGTGATTGC TGACCTAGAA 
TGTCGTTATT CCTGGGATCA CTCTCCTCTC AGTATGTTTC GACACTACAC CGTTTATTTG 
GACTTGTATG CTGTTATTAA TGACTTAAGT TCCAAAATCG AAGCCACAAG GTTAGGTGTT 
ACAGCACTTG AGCTGCGGTT TCGTGGAGCA AAGGTGGTTT CCCGCTTATC TGAAGGGGTG 
TCTCACGTAA TCATCGGGGA AGATCAGAGA CGAGTCAGTG ACTTTAAAAC GTTCAGAAGA 
ACGCTTAAGA AAAAGTTTAA AATCCTGCAG GAACGTTGGG TGATCGATTC AGTAGACAAG 
TGTGAACTGC AGGAAGAAAA CCTGTATTTG CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig4 ( XM_006253477.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253539.1 Rattus norvegicus DNA ligase 4 (Lig4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa00169
Clone ID Related Accession (Same CDS sequence)	XM_006253477.3 , XM_017600050.1 , NM_001106095.1
Accession Version	XM_006253477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCT CACAAGCCTC ACGAACTGTC GCAGCTCATG TCCCCTTTGC AGACTTGTGC 
TCCACCTTAG AACGAATACA GAAAGGTAAA GACCGTGCAG AAAAAATCAG GCACTTCAAG 
GAGTTTCTGG ATTCTTGGAG AAAATTTCAT GATGCCCTTC ATAAGAACAA GAAGGATGTT 
CCGGACTCTT TTTACCCAGC AATGAGACTT ATTCTCCCTC AGCTAGAGAG AGAGAGGATG 
GCTTACGGAA TCAAAGAAAC CATGCTGGCT AAGCTTTACA TCGAATTGCT CAATTTACCA 
AGAGAAGGCA AGGATGCCCA GAAGCTCCTG AATTACCGAA CCCCCAGTGG AGCTCGCTCA 
GATGCTGGGG ACTTTGCCAC GATTGCATAC TTTGTTTTGA AGCCAAGGTG CTTACAGAAA 
GGAAGCTTAA CCATACAGAA GGTGAATGAA CTCTTAGACT TAGTTGCCAG CAATAACTCT 
GGCAAAAGAA AAGATCTAGT GAAAAAGAGC CTTCTTCAGT TAATAACCCA GTGTTCAGCA 
CTCGAGCAAA AGTGGCTGAT TCGCATGATT ATTAAGGACT TAAAGCTTGG TGTCAGTCAG 
CACACGATAT TGTCCATCTT CCACAATGAT GCAGTCGAGT TGCACAATGT CACCACAGAT 
CTGGAAAAGG TCTGTAGGCA GCTACATGAC CCCTCCGTAG GGCTCAGCGA CATCTCCATC 
ACTTTGTTCT CTGCCTTTAG GCCAATGCTA GCTGCTGTAG CAGATGTGGA GCGTGTGGAG 
AAGGACATGA AGCAGCAGAG TTTCTACATC GAAACTAAGC TCGATGGCGA GCGCATGCAG 
ATGCACAAGG ATGGCGCCCT GTACCGGTAC TTCTCCAGAA ATGGTTACAA CTACACCGAT 
CAGTTCGGCG AATCTCCACA GGAAGGCTCT CTCACCCCGT TTATTCACAA CGCATTCAAG 
ACAGATGTGC AAGTGTGCAT CCTTGATGGC GAGATGATGG CCTACAACCC AACGACACAG 
ACTTTCATGC AGAAGGGGGT CAAGTTTGAT ATCAAAAGGA TGGTCGAAGA TTCTGACCTA 
CAGACATGCT ACTGTGTTTT CGATGTGCTG ATGGTTAATA ATAAGAAGCT AGGGCGTGAG 
ACTCTCAGGA AGAGGTATGA TATCCTTAGT AGCACTTTCA CACCCATACA AGGTCGAATA 
GAGATAGTGC AGAAAACTCA AGCTCAGACA AAGCAGGAAG TAGTGGATGC ATTAAATGAT 
GCCATAGACA AGAGAGAAGA GGGGATCATG GTTAAACACC CTCTGTCCAT TTACAAGCCA 
GACAAAAGAG GTGAAGGGTG GCTAAAGATT AAACCAGAGT ATGTCACTGG ATTGATGGAT 
GAATTAGACC TCTTAATTGT TGGGGGCTAC TGGGGTAAAG GTTCTCGAGG TGGCATGATG 
TCTCACTTTC TGTGTGCAGT GGCAGAGACA CCACCTCCTG GTGACAAGCC ATCTGTGTTC 
CACACTCTGT GCCGTGTTGG GTCGGGTTAC ACGATGAAAG AACTCTATGA CCTGGGCTTG 
AAATTGGCAA AATACTGGAA GCCTTTCCAA AGAAAATCTC CACCAAGCTG TATTTTGTGT 
GGCACAGAGA AGCCAGAAGT GTACATTGAA CCCCAGAACT CTGTCATCGT TCAGATCAAG 
GCGGCAGAGA TCGTCCCCAG TGACATGTAC AAGACTGGCT CTACCTTGCG CTTCCCACGC 
ATCGAGAAGA TCAGAGATGA CAAAGAGTGG CATGACTGTA TGACGCTGGA TGACCTGGAG 
CAGCTGAGGG GGAAAGCATC TGGGAAGCTT GCCACAAAAC ACCTTCACAT AGGTGACGAT 
GACGAACCCA GAGAAAAAAG GCGGAAACCC ATCTCTAAGA CGAAGAAAAC CATTGGAATC 
ATTGAACACC TAAAAGCGCC TAACCTTTCT AACATAAACA AAGTTTCTAA TGTATTTGAA 
GATGTTGAGT TTTGTGTCAT GAGTGGATTA GATGGTTATC CAAAGCCTGA CCTAGAGAAC 
AAAATTGCAG AATTTGGTGG TTATATAGTC CAGAATCCAG GCCCAGATAC ATACTGTGTG 
ATTGCAGGTT CTGAGAACAT TAGGGTCAGA AACATCATCT CTTCAGATAG GCATGATGTT 
GTCAAGCCCG AGTGGCTTTT AGAGTGTTTT AAAACAAAAA CATGTGTGCC ATGGCAACCC 
CGCTTTATGA TTCACATGTG CCCATCAACA AAGCAGCATT TTGCCCGTGA ATATGACTGC 
TATGGTGATA GCTATTTCGT TGATGCAGAT TTGGATCAAT TGAAAGAAGT GTTTTTAAGA 
ATTAAACCCA GTGAGCAGCA GACTCCTGAA GAAATGGCCC CTGTGATTGC TGACCTAGAA 
TGTCGTTATT CCTGGGATCA CTCTCCTCTC AGTATGTTTC GACACTACAC CGTTTATTTG 
GACTTGTATG CTGTTATTAA TGACTTAAGT TCCAAAATCG AAGCCACAAG GTTAGGTGTT 
ACAGCACTTG AGCTGCGGTT TCGTGGAGCA AAGGTGGTTT CCCGCTTATC TGAAGGGGTG 
TCTCACGTAA TCATCGGGGA AGATCAGAGA CGAGTCAGTG ACTTTAAAAC GTTCAGAAGA 
ACGCTTAAGA AAAAGTTTAA AATCCTGCAG GAACGTTGGG TGATCGATTC AGTAGACAAG 
TGTGAACTGC AGGAAGAAAA CCTGTATTTG CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253539.1
CDS	246..2981
Translation	MASSQASRTVAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKNKKDVPDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGARSDAGDFATIAYFVLKPRCLQKGSLTIQKVNELLDLVASNNSGKRKDLVKKSLLQLITQCSALEQKWLIRMIIKDLKLGVSQHTILSIFHNDAVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFRPMLAAVADVERVEKDMKQQSFYIETKLDGERMQMHKDGALYRYFSRNGYNYTDQFGESPQEGSLTPFIHNAFKTDVQVCILDGEMMAYNPTTQTFMQKGVKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGRETLRKRYDILSSTFTPIQGRIEIVQKTQAQTKQEVVDALNDAIDKREEGIMVKHPLSIYKPDKRGEGWLKIKPEYVTGLMDELDLLIVGGYWGKGSRGGMMSHFLCAVAETPPPGDKPSVFHTLCRVGSGYTMKELYDLGLKLAKYWKPFQRKSPPSCILCGTEKPEVYIEPQNSVIVQIKAAEIVPSDMYKTGSTLRFPRIEKIRDDKEWHDCMTLDDLEQLRGKASGKLATKHLHIGDDDEPREKRRKPISKTKKTIGIIEHLKAPNLSNINKVSNVFEDVEFCVMSGLDGYPKPDLENKIAEFGGYIVQNPGPDTYCVIAGSENIRVRNIISSDRHDVVKPEWLLECFKTKTCVPWQPRFMIHMCPSTKQHFAREYDCYGDSYFVDADLDQLKEVFLRIKPSEQQTPEEMAPVIADLECRYSWDHSPLSMFRHYTVYLDLYAVINDLSSKIEATRLGVTALELRFRGAKVVSRLSEGVSHVIIGEDQRRVSDFKTFRRTLKKKFKILQERWVIDSVDKCELQEENLYLL

Target ORF information:

RefSeq Version	XM_006253477.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 4 (Lig4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253477.3

ORF Insert Sequence:

ATGGCTTCCT CACAAGCCTC ACGAACTGTC GCAGCTCATG TCCCCTTTGC AGACTTGTGC 
TCCACCTTAG AACGAATACA GAAAGGTAAA GACCGTGCAG AAAAAATCAG GCACTTCAAG 
GAGTTTCTGG ATTCTTGGAG AAAATTTCAT GATGCCCTTC ATAAGAACAA GAAGGATGTT 
CCGGACTCTT TTTACCCAGC AATGAGACTT ATTCTCCCTC AGCTAGAGAG AGAGAGGATG 
GCTTACGGAA TCAAAGAAAC CATGCTGGCT AAGCTTTACA TCGAATTGCT CAATTTACCA 
AGAGAAGGCA AGGATGCCCA GAAGCTCCTG AATTACCGAA CCCCCAGTGG AGCTCGCTCA 
GATGCTGGGG ACTTTGCCAC GATTGCATAC TTTGTTTTGA AGCCAAGGTG CTTACAGAAA 
GGAAGCTTAA CCATACAGAA GGTGAATGAA CTCTTAGACT TAGTTGCCAG CAATAACTCT 
GGCAAAAGAA AAGATCTAGT GAAAAAGAGC CTTCTTCAGT TAATAACCCA GTGTTCAGCA 
CTCGAGCAAA AGTGGCTGAT TCGCATGATT ATTAAGGACT TAAAGCTTGG TGTCAGTCAG 
CACACGATAT TGTCCATCTT CCACAATGAT GCAGTCGAGT TGCACAATGT CACCACAGAT 
CTGGAAAAGG TCTGTAGGCA GCTACATGAC CCCTCCGTAG GGCTCAGCGA CATCTCCATC 
ACTTTGTTCT CTGCCTTTAG GCCAATGCTA GCTGCTGTAG CAGATGTGGA GCGTGTGGAG 
AAGGACATGA AGCAGCAGAG TTTCTACATC GAAACTAAGC TCGATGGCGA GCGCATGCAG 
ATGCACAAGG ATGGCGCCCT GTACCGGTAC TTCTCCAGAA ATGGTTACAA CTACACCGAT 
CAGTTCGGCG AATCTCCACA GGAAGGCTCT CTCACCCCGT TTATTCACAA CGCATTCAAG 
ACAGATGTGC AAGTGTGCAT CCTTGATGGC GAGATGATGG CCTACAACCC AACGACACAG 
ACTTTCATGC AGAAGGGGGT CAAGTTTGAT ATCAAAAGGA TGGTCGAAGA TTCTGACCTA 
CAGACATGCT ACTGTGTTTT CGATGTGCTG ATGGTTAATA ATAAGAAGCT AGGGCGTGAG 
ACTCTCAGGA AGAGGTATGA TATCCTTAGT AGCACTTTCA CACCCATACA AGGTCGAATA 
GAGATAGTGC AGAAAACTCA AGCTCAGACA AAGCAGGAAG TAGTGGATGC ATTAAATGAT 
GCCATAGACA AGAGAGAAGA GGGGATCATG GTTAAACACC CTCTGTCCAT TTACAAGCCA 
GACAAAAGAG GTGAAGGGTG GCTAAAGATT AAACCAGAGT ATGTCACTGG ATTGATGGAT 
GAATTAGACC TCTTAATTGT TGGGGGCTAC TGGGGTAAAG GTTCTCGAGG TGGCATGATG 
TCTCACTTTC TGTGTGCAGT GGCAGAGACA CCACCTCCTG GTGACAAGCC ATCTGTGTTC 
CACACTCTGT GCCGTGTTGG GTCGGGTTAC ACGATGAAAG AACTCTATGA CCTGGGCTTG 
AAATTGGCAA AATACTGGAA GCCTTTCCAA AGAAAATCTC CACCAAGCTG TATTTTGTGT 
GGCACAGAGA AGCCAGAAGT GTACATTGAA CCCCAGAACT CTGTCATCGT TCAGATCAAG 
GCGGCAGAGA TCGTCCCCAG TGACATGTAC AAGACTGGCT CTACCTTGCG CTTCCCACGC 
ATCGAGAAGA TCAGAGATGA CAAAGAGTGG CATGACTGTA TGACGCTGGA TGACCTGGAG 
CAGCTGAGGG GGAAAGCATC TGGGAAGCTT GCCACAAAAC ACCTTCACAT AGGTGACGAT 
GACGAACCCA GAGAAAAAAG GCGGAAACCC ATCTCTAAGA CGAAGAAAAC CATTGGAATC 
ATTGAACACC TAAAAGCGCC TAACCTTTCT AACATAAACA AAGTTTCTAA TGTATTTGAA 
GATGTTGAGT TTTGTGTCAT GAGTGGATTA GATGGTTATC CAAAGCCTGA CCTAGAGAAC 
AAAATTGCAG AATTTGGTGG TTATATAGTC CAGAATCCAG GCCCAGATAC ATACTGTGTG 
ATTGCAGGTT CTGAGAACAT TAGGGTCAGA AACATCATCT CTTCAGATAG GCATGATGTT 
GTCAAGCCCG AGTGGCTTTT AGAGTGTTTT AAAACAAAAA CATGTGTGCC ATGGCAACCC 
CGCTTTATGA TTCACATGTG CCCATCAACA AAGCAGCATT TTGCCCGTGA ATATGACTGC 
TATGGTGATA GCTATTTCGT TGATGCAGAT TTGGATCAAT TGAAAGAAGT GTTTTTAAGA 
ATTAAACCCA GTGAGCAGCA GACTCCTGAA GAAATGGCCC CTGTGATTGC TGACCTAGAA 
TGTCGTTATT CCTGGGATCA CTCTCCTCTC AGTATGTTTC GACACTACAC CGTTTATTTG 
GACTTGTATG CTGTTATTAA TGACTTAAGT TCCAAAATCG AAGCCACAAG GTTAGGTGTT 
ACAGCACTTG AGCTGCGGTT TCGTGGAGCA AAGGTGGTTT CCCGCTTATC TGAAGGGGTG 
TCTCACGTAA TCATCGGGGA AGATCAGAGA CGAGTCAGTG ACTTTAAAAC GTTCAGAAGA 
ACGCTTAAGA AAAAGTTTAA AATCCTGCAG GAACGTTGGG TGATCGATTC AGTAGACAAG 
TGTGAACTGC AGGAAGAAAA CCTGTATTTG CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig4 ( XM_017600050.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455539.1 Rattus norvegicus DNA ligase 4 (Lig4), transcript variant X1, mRNA.

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CloneID	ORa00169
Clone ID Related Accession (Same CDS sequence)	XM_006253477.3 , XM_017600050.1 , NM_001106095.1
Accession Version	XM_017600050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA ligase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCT CACAAGCCTC ACGAACTGTC GCAGCTCATG TCCCCTTTGC AGACTTGTGC 
TCCACCTTAG AACGAATACA GAAAGGTAAA GACCGTGCAG AAAAAATCAG GCACTTCAAG 
GAGTTTCTGG ATTCTTGGAG AAAATTTCAT GATGCCCTTC ATAAGAACAA GAAGGATGTT 
CCGGACTCTT TTTACCCAGC AATGAGACTT ATTCTCCCTC AGCTAGAGAG AGAGAGGATG 
GCTTACGGAA TCAAAGAAAC CATGCTGGCT AAGCTTTACA TCGAATTGCT CAATTTACCA 
AGAGAAGGCA AGGATGCCCA GAAGCTCCTG AATTACCGAA CCCCCAGTGG AGCTCGCTCA 
GATGCTGGGG ACTTTGCCAC GATTGCATAC TTTGTTTTGA AGCCAAGGTG CTTACAGAAA 
GGAAGCTTAA CCATACAGAA GGTGAATGAA CTCTTAGACT TAGTTGCCAG CAATAACTCT 
GGCAAAAGAA AAGATCTAGT GAAAAAGAGC CTTCTTCAGT TAATAACCCA GTGTTCAGCA 
CTCGAGCAAA AGTGGCTGAT TCGCATGATT ATTAAGGACT TAAAGCTTGG TGTCAGTCAG 
CACACGATAT TGTCCATCTT CCACAATGAT GCAGTCGAGT TGCACAATGT CACCACAGAT 
CTGGAAAAGG TCTGTAGGCA GCTACATGAC CCCTCCGTAG GGCTCAGCGA CATCTCCATC 
ACTTTGTTCT CTGCCTTTAG GCCAATGCTA GCTGCTGTAG CAGATGTGGA GCGTGTGGAG 
AAGGACATGA AGCAGCAGAG TTTCTACATC GAAACTAAGC TCGATGGCGA GCGCATGCAG 
ATGCACAAGG ATGGCGCCCT GTACCGGTAC TTCTCCAGAA ATGGTTACAA CTACACCGAT 
CAGTTCGGCG AATCTCCACA GGAAGGCTCT CTCACCCCGT TTATTCACAA CGCATTCAAG 
ACAGATGTGC AAGTGTGCAT CCTTGATGGC GAGATGATGG CCTACAACCC AACGACACAG 
ACTTTCATGC AGAAGGGGGT CAAGTTTGAT ATCAAAAGGA TGGTCGAAGA TTCTGACCTA 
CAGACATGCT ACTGTGTTTT CGATGTGCTG ATGGTTAATA ATAAGAAGCT AGGGCGTGAG 
ACTCTCAGGA AGAGGTATGA TATCCTTAGT AGCACTTTCA CACCCATACA AGGTCGAATA 
GAGATAGTGC AGAAAACTCA AGCTCAGACA AAGCAGGAAG TAGTGGATGC ATTAAATGAT 
GCCATAGACA AGAGAGAAGA GGGGATCATG GTTAAACACC CTCTGTCCAT TTACAAGCCA 
GACAAAAGAG GTGAAGGGTG GCTAAAGATT AAACCAGAGT ATGTCACTGG ATTGATGGAT 
GAATTAGACC TCTTAATTGT TGGGGGCTAC TGGGGTAAAG GTTCTCGAGG TGGCATGATG 
TCTCACTTTC TGTGTGCAGT GGCAGAGACA CCACCTCCTG GTGACAAGCC ATCTGTGTTC 
CACACTCTGT GCCGTGTTGG GTCGGGTTAC ACGATGAAAG AACTCTATGA CCTGGGCTTG 
AAATTGGCAA AATACTGGAA GCCTTTCCAA AGAAAATCTC CACCAAGCTG TATTTTGTGT 
GGCACAGAGA AGCCAGAAGT GTACATTGAA CCCCAGAACT CTGTCATCGT TCAGATCAAG 
GCGGCAGAGA TCGTCCCCAG TGACATGTAC AAGACTGGCT CTACCTTGCG CTTCCCACGC 
ATCGAGAAGA TCAGAGATGA CAAAGAGTGG CATGACTGTA TGACGCTGGA TGACCTGGAG 
CAGCTGAGGG GGAAAGCATC TGGGAAGCTT GCCACAAAAC ACCTTCACAT AGGTGACGAT 
GACGAACCCA GAGAAAAAAG GCGGAAACCC ATCTCTAAGA CGAAGAAAAC CATTGGAATC 
ATTGAACACC TAAAAGCGCC TAACCTTTCT AACATAAACA AAGTTTCTAA TGTATTTGAA 
GATGTTGAGT TTTGTGTCAT GAGTGGATTA GATGGTTATC CAAAGCCTGA CCTAGAGAAC 
AAAATTGCAG AATTTGGTGG TTATATAGTC CAGAATCCAG GCCCAGATAC ATACTGTGTG 
ATTGCAGGTT CTGAGAACAT TAGGGTCAGA AACATCATCT CTTCAGATAG GCATGATGTT 
GTCAAGCCCG AGTGGCTTTT AGAGTGTTTT AAAACAAAAA CATGTGTGCC ATGGCAACCC 
CGCTTTATGA TTCACATGTG CCCATCAACA AAGCAGCATT TTGCCCGTGA ATATGACTGC 
TATGGTGATA GCTATTTCGT TGATGCAGAT TTGGATCAAT TGAAAGAAGT GTTTTTAAGA 
ATTAAACCCA GTGAGCAGCA GACTCCTGAA GAAATGGCCC CTGTGATTGC TGACCTAGAA 
TGTCGTTATT CCTGGGATCA CTCTCCTCTC AGTATGTTTC GACACTACAC CGTTTATTTG 
GACTTGTATG CTGTTATTAA TGACTTAAGT TCCAAAATCG AAGCCACAAG GTTAGGTGTT 
ACAGCACTTG AGCTGCGGTT TCGTGGAGCA AAGGTGGTTT CCCGCTTATC TGAAGGGGTG 
TCTCACGTAA TCATCGGGGA AGATCAGAGA CGAGTCAGTG ACTTTAAAAC GTTCAGAAGA 
ACGCTTAAGA AAAAGTTTAA AATCCTGCAG GAACGTTGGG TGATCGATTC AGTAGACAAG 
TGTGAACTGC AGGAAGAAAA CCTGTATTTG CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455539.1
CDS	2895..5630
Translation	MASSQASRTVAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKNKKDVPDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGARSDAGDFATIAYFVLKPRCLQKGSLTIQKVNELLDLVASNNSGKRKDLVKKSLLQLITQCSALEQKWLIRMIIKDLKLGVSQHTILSIFHNDAVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFRPMLAAVADVERVEKDMKQQSFYIETKLDGERMQMHKDGALYRYFSRNGYNYTDQFGESPQEGSLTPFIHNAFKTDVQVCILDGEMMAYNPTTQTFMQKGVKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGRETLRKRYDILSSTFTPIQGRIEIVQKTQAQTKQEVVDALNDAIDKREEGIMVKHPLSIYKPDKRGEGWLKIKPEYVTGLMDELDLLIVGGYWGKGSRGGMMSHFLCAVAETPPPGDKPSVFHTLCRVGSGYTMKELYDLGLKLAKYWKPFQRKSPPSCILCGTEKPEVYIEPQNSVIVQIKAAEIVPSDMYKTGSTLRFPRIEKIRDDKEWHDCMTLDDLEQLRGKASGKLATKHLHIGDDDEPREKRRKPISKTKKTIGIIEHLKAPNLSNINKVSNVFEDVEFCVMSGLDGYPKPDLENKIAEFGGYIVQNPGPDTYCVIAGSENIRVRNIISSDRHDVVKPEWLLECFKTKTCVPWQPRFMIHMCPSTKQHFAREYDCYGDSYFVDADLDQLKEVFLRIKPSEQQTPEEMAPVIADLECRYSWDHSPLSMFRHYTVYLDLYAVINDLSSKIEATRLGVTALELRFRGAKVVSRLSEGVSHVIIGEDQRRVSDFKTFRRTLKKKFKILQERWVIDSVDKCELQEENLYLL

Target ORF information:

RefSeq Version	XM_017600050.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA ligase 4 (Lig4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600050.1

ORF Insert Sequence:

ATGGCTTCCT CACAAGCCTC ACGAACTGTC GCAGCTCATG TCCCCTTTGC AGACTTGTGC 
TCCACCTTAG AACGAATACA GAAAGGTAAA GACCGTGCAG AAAAAATCAG GCACTTCAAG 
GAGTTTCTGG ATTCTTGGAG AAAATTTCAT GATGCCCTTC ATAAGAACAA GAAGGATGTT 
CCGGACTCTT TTTACCCAGC AATGAGACTT ATTCTCCCTC AGCTAGAGAG AGAGAGGATG 
GCTTACGGAA TCAAAGAAAC CATGCTGGCT AAGCTTTACA TCGAATTGCT CAATTTACCA 
AGAGAAGGCA AGGATGCCCA GAAGCTCCTG AATTACCGAA CCCCCAGTGG AGCTCGCTCA 
GATGCTGGGG ACTTTGCCAC GATTGCATAC TTTGTTTTGA AGCCAAGGTG CTTACAGAAA 
GGAAGCTTAA CCATACAGAA GGTGAATGAA CTCTTAGACT TAGTTGCCAG CAATAACTCT 
GGCAAAAGAA AAGATCTAGT GAAAAAGAGC CTTCTTCAGT TAATAACCCA GTGTTCAGCA 
CTCGAGCAAA AGTGGCTGAT TCGCATGATT ATTAAGGACT TAAAGCTTGG TGTCAGTCAG 
CACACGATAT TGTCCATCTT CCACAATGAT GCAGTCGAGT TGCACAATGT CACCACAGAT 
CTGGAAAAGG TCTGTAGGCA GCTACATGAC CCCTCCGTAG GGCTCAGCGA CATCTCCATC 
ACTTTGTTCT CTGCCTTTAG GCCAATGCTA GCTGCTGTAG CAGATGTGGA GCGTGTGGAG 
AAGGACATGA AGCAGCAGAG TTTCTACATC GAAACTAAGC TCGATGGCGA GCGCATGCAG 
ATGCACAAGG ATGGCGCCCT GTACCGGTAC TTCTCCAGAA ATGGTTACAA CTACACCGAT 
CAGTTCGGCG AATCTCCACA GGAAGGCTCT CTCACCCCGT TTATTCACAA CGCATTCAAG 
ACAGATGTGC AAGTGTGCAT CCTTGATGGC GAGATGATGG CCTACAACCC AACGACACAG 
ACTTTCATGC AGAAGGGGGT CAAGTTTGAT ATCAAAAGGA TGGTCGAAGA TTCTGACCTA 
CAGACATGCT ACTGTGTTTT CGATGTGCTG ATGGTTAATA ATAAGAAGCT AGGGCGTGAG 
ACTCTCAGGA AGAGGTATGA TATCCTTAGT AGCACTTTCA CACCCATACA AGGTCGAATA 
GAGATAGTGC AGAAAACTCA AGCTCAGACA AAGCAGGAAG TAGTGGATGC ATTAAATGAT 
GCCATAGACA AGAGAGAAGA GGGGATCATG GTTAAACACC CTCTGTCCAT TTACAAGCCA 
GACAAAAGAG GTGAAGGGTG GCTAAAGATT AAACCAGAGT ATGTCACTGG ATTGATGGAT 
GAATTAGACC TCTTAATTGT TGGGGGCTAC TGGGGTAAAG GTTCTCGAGG TGGCATGATG 
TCTCACTTTC TGTGTGCAGT GGCAGAGACA CCACCTCCTG GTGACAAGCC ATCTGTGTTC 
CACACTCTGT GCCGTGTTGG GTCGGGTTAC ACGATGAAAG AACTCTATGA CCTGGGCTTG 
AAATTGGCAA AATACTGGAA GCCTTTCCAA AGAAAATCTC CACCAAGCTG TATTTTGTGT 
GGCACAGAGA AGCCAGAAGT GTACATTGAA CCCCAGAACT CTGTCATCGT TCAGATCAAG 
GCGGCAGAGA TCGTCCCCAG TGACATGTAC AAGACTGGCT CTACCTTGCG CTTCCCACGC 
ATCGAGAAGA TCAGAGATGA CAAAGAGTGG CATGACTGTA TGACGCTGGA TGACCTGGAG 
CAGCTGAGGG GGAAAGCATC TGGGAAGCTT GCCACAAAAC ACCTTCACAT AGGTGACGAT 
GACGAACCCA GAGAAAAAAG GCGGAAACCC ATCTCTAAGA CGAAGAAAAC CATTGGAATC 
ATTGAACACC TAAAAGCGCC TAACCTTTCT AACATAAACA AAGTTTCTAA TGTATTTGAA 
GATGTTGAGT TTTGTGTCAT GAGTGGATTA GATGGTTATC CAAAGCCTGA CCTAGAGAAC 
AAAATTGCAG AATTTGGTGG TTATATAGTC CAGAATCCAG GCCCAGATAC ATACTGTGTG 
ATTGCAGGTT CTGAGAACAT TAGGGTCAGA AACATCATCT CTTCAGATAG GCATGATGTT 
GTCAAGCCCG AGTGGCTTTT AGAGTGTTTT AAAACAAAAA CATGTGTGCC ATGGCAACCC 
CGCTTTATGA TTCACATGTG CCCATCAACA AAGCAGCATT TTGCCCGTGA ATATGACTGC 
TATGGTGATA GCTATTTCGT TGATGCAGAT TTGGATCAAT TGAAAGAAGT GTTTTTAAGA 
ATTAAACCCA GTGAGCAGCA GACTCCTGAA GAAATGGCCC CTGTGATTGC TGACCTAGAA 
TGTCGTTATT CCTGGGATCA CTCTCCTCTC AGTATGTTTC GACACTACAC CGTTTATTTG 
GACTTGTATG CTGTTATTAA TGACTTAAGT TCCAAAATCG AAGCCACAAG GTTAGGTGTT 
ACAGCACTTG AGCTGCGGTT TCGTGGAGCA AAGGTGGTTT CCCGCTTATC TGAAGGGGTG 
TCTCACGTAA TCATCGGGGA AGATCAGAGA CGAGTCAGTG ACTTTAAAAC GTTCAGAAGA 
ACGCTTAAGA AAAAGTTTAA AATCCTGCAG GAACGTTGGG TGATCGATTC AGTAGACAAG 
TGTGAACTGC AGGAAGAAAA CCTGTATTTG CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during late mitosis.
Nature communications9(1)728(2018 02)
Hong Y,Sonneville R,Wang B,Scheidt V,Meier B,Woglar A,Demetriou S,Labib K,Jantsch V,Gartner A

Ligase-4 Deficiency Causes Distinctive Immune Abnormalities in Asymptomatic Individuals.
Journal of clinical immunology36(4)341-53(2016 05)
Felgentreff K,Baxi SN,Lee YN,Dobbs K,Henderson LA,Csomos K,Tsitsikov EN,Armanios M,Walter JE,Notarangelo LD

XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair.
Cell death and differentiation22(6)890-7(2015 Jun)
Craxton A,Somers J,Munnur D,Jukes-Jones R,Cain K,Malewicz M

DNA ligase III and DNA ligase IV carry out genetically distinct forms of end joining in human somatic cells.
DNA repair2197-110(2014 Sep)
Oh S,Harvey A,Zimbric J,Wang Y,Nguyen T,Jackson PJ,Hendrickson EA

Altered regulation of DNA ligase IV activity by aberrant promoter DNA methylation and gene amplification in colorectal cancer.
Human molecular genetics23(8)2043-54(2014 Apr)
Kuhmann C,Li C,Kloor M,Salou M,Weigel C,Schmidt CR,Ng LW,Tsui WW,Leung SY,Yuen ST,Becker N,Weichenhan D,Plass C,Schmezer P,Chan TL,Popanda O

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Lig4 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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