Akr1c14 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akr1c14
Entrez Gene ID	191574
Full Name	aldo-keto reductase family 1, member C14
Synonyms	Akr1c9
General protein information	Preferred Names aldo-keto reductase family 1, member C14 Names 3-alpha-hydroxysteroid dehydrogenase 3-alpha-HSD hydroxyprostaglandin dehydrogenase steroid 3-alpha-dehydrogenase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.2
Summary	catalyzes the conversion of 5 alpha-dihydrotestosterone to 3 alpha-androstanediol in steroid metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138547.3	NP_612556.1	3-alpha-hydroxysteroid dehydrogenase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Akr1c14	NP_612556.1
Mus musculus (house mouse)	Akr1c14	NP_598833.1

Gene Ontology
Bibliography

Related articles in PubMed

Ziram inhibits rat neurosteroidogenic 5α-reductase 1 and 3α-hydroxysteroid dehydrogenase.
Su Y, Li H, Chen X, Wang Y, Li X, Sun J, Ge RS
Toxicology mechanisms and methods28(1)38-44(2018 Jan)

Butylated hydroxyanisole alters rat 5α-reductase and 3α-hydroxysteroid dehydrogenase: Implications for influences of neurosteroidogenesis.
Guo J, Li L, Zhou S, Su Y, Li X, Sun J, Ge RS
Neuroscience letters653132-138(2017 Jul)

Semicomprehensive analysis of the postnatal age-related changes in the mRNA expression of sex steroidogenic enzymes and sex steroid receptors in the male rat hippocampus.
Kimoto T, Ishii H, Higo S, Hojo Y, Kawato S
Endocrinology151(12)5795-806(2010 Dec)

Elucidation of a complete kinetic mechanism for a mammalian hydroxysteroid dehydrogenase (HSD) and identification of all enzyme forms on the reaction coordinate: the example of rat liver 3alpha-HSD (AKR1C9).
Cooper WC, Jin Y, Penning TM
The Journal of biological chemistry282(46)33484-93(2007 Nov)

Molecular cloning of rat liver 3α-hydroxysteroid dehydrogenase and identification of structurally related proteins from rat lung and kidney.
Cheng KC
The Journal of steroid biochemistry and molecular biology43(8)1083-8(1992 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Akr1c14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akr1c14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01630	NM_138547.3 Latest version!	Rattus norvegicus aldo-keto reductase family 1, member C14 (Akr1c14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akr1c14 ( NM_138547.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612556.1 Rattus norvegicus aldo-keto reductase family 1, member C14 (Akr1c14), mRNA.

Price & Availability↑

CloneID	ORa01630
Clone ID Related Accession (Same CDS sequence)	NM_138547.3
Accession Version	NM_138547.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	3-alpha-hydroxysteroid dehydrogenase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07028443.1 and BC091123.1. On Mar 12, 2015 this sequence version replaced NM_138547.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091123.1, M64393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906353, SAMN01906355 [ECO:0000348] ##Evidence-Data-END##

ATGGATTCCA TATCTCTGCG TGTAGCACTA AATGATGGTA ACTTCATTCC TGTACTGGGG 
TTTGGAACCA CTGTGCCTGA GAAGGTTGCT AAGGATGAAG TTATCAAGGC TACTAAAATA 
GCTATAGATA ATGGATTCCG CCATTTTGAC TCTGCTTATT TGTACGAAGT AGAAGAGGAA 
GTGGGCCAAG CCATTAGAAG CAAGATTGAA GACGGCACTG TGAAGAGAGA AGATATATTC 
TATACTTCAA AGCTTTGGAG CACTTTCCAT AGACCAGAGC TGGTCCGAAC TTGCTTGGAA 
AAGACACTGA AAAGCACTCA ACTGGACTAT GTGGATCTTT ATATTATTCA TTTCCCAATG 
GCTTTGCAGC CTGGAGATAT ATTTTTCCCA CGAGATGAGC ATGGAAAACT ATTGTTTGAA 
ACAGTGGATA TCTGTGACAC ATGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGATTGGCC 
AAGTCTATTG GGGTGTCCAA CTTTAACTGC AGGCAGCTGG AGAGGATTCT GAATAAGCCA 
GGGCTCAAAT ACAAGCCTGT GTGCAACCAG GTGGAATGTC ACCTTTATCT CAACCAGAGC 
AAAATGCTGG ACTATTGTAA GTCAAAAGAC ATCATTCTGG TTTCCTACTG CACGCTGGGA 
AGTTCACGAG ACAAAACATG GGTGGATCAG AAAAGTCCAG TTCTCCTAGA TGATCCAGTT 
CTTTGTGCCA TAGCAAAGAA GTACAAGCAA ACCCCAGCCC TAGTTGCCCT TCGCTACCAG 
CTGCAGCGTG GGGTTGTGCC CCTGATCAGG AGTTTCAACG CGAAGCGGAT CAAAGAGCTA 
ACACAGGTTT TTGAATTCCA GTTGGCTTCA GAGGACATGA AAGCCCTGGA TGGCTTGAAC 
AGAAATTTCA GATACAACAA TGCAAAATAT TTTGATGACC ATCCCAATCA TCCATTTACT 
GATGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612556.1
CDS	58..1026
Translation	MDSISLRVALNDGNFIPVLGFGTTVPEKVAKDEVIKATKIAIDNGFRHFDSAYLYEVEEEVGQAIRSKIEDGTVKREDIFYTSKLWSTFHRPELVRTCLEKTLKSTQLDYVDLYIIHFPMALQPGDIFFPRDEHGKLLFETVDICDTWEAMEKCKDAGLAKSIGVSNFNCRQLERILNKPGLKYKPVCNQVECHLYLNQSKMLDYCKSKDIILVSYCTLGSSRDKTWVDQKSPVLLDDPVLCAIAKKYKQTPALVALRYQLQRGVVPLIRSFNAKRIKELTQVFEFQLASEDMKALDGLNRNFRYNNAKYFDDHPNHPFTDE

Target ORF information:

RefSeq Version	NM_138547.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aldo-keto reductase family 1, member C14 (Akr1c14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138547.3

ORF Insert Sequence:

ATGGATTCCA TATCTCTGCG TGTAGCACTA AATGATGGTA ACTTCATTCC TGTACTGGGG 
TTTGGAACCA CTGTGCCTGA GAAGGTTGCT AAGGATGAAG TTATCAAGGC TACTAAAATA 
GCTATAGATA ATGGATTCCG CCATTTTGAC TCTGCTTATT TGTACGAAGT AGAAGAGGAA 
GTGGGCCAAG CCATTAGAAG CAAGATTGAA GACGGCACTG TGAAGAGAGA AGATATATTC 
TATACTTCAA AGCTTTGGAG CACTTTCCAT AGACCAGAGC TGGTCCGAAC TTGCTTGGAA 
AAGACACTGA AAAGCACTCA ACTGGACTAT GTGGATCTTT ATATTATTCA TTTCCCAATG 
GCTTTGCAGC CTGGAGATAT ATTTTTCCCA CGAGATGAGC ATGGAAAACT ATTGTTTGAA 
ACAGTGGATA TCTGTGACAC ATGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGATTGGCC 
AAGTCTATTG GGGTGTCCAA CTTTAACTGC AGGCAGCTGG AGAGGATTCT GAATAAGCCA 
GGGCTCAAAT ACAAGCCTGT GTGCAACCAG GTGGAATGTC ACCTTTATCT CAACCAGAGC 
AAAATGCTGG ACTATTGTAA GTCAAAAGAC ATCATTCTGG TTTCCTACTG CACGCTGGGA 
AGTTCACGAG ACAAAACATG GGTGGATCAG AAAAGTCCAG TTCTCCTAGA TGATCCAGTT 
CTTTGTGCCA TAGCAAAGAA GTACAAGCAA ACCCCAGCCC TAGTTGCCCT TCGCTACCAG 
CTGCAGCGTG GGGTTGTGCC CCTGATCAGG AGTTTCAACG CGAAGCGGAT CAAAGAGCTA 
ACACAGGTTT TTGAATTCCA GTTGGCTTCA GAGGACATGA AAGCCCTGGA TGGCTTGAAC 
AGAAATTTCA GATACAACAA TGCAAAATAT TTTGATGACC ATCCCAATCA TCCATTTACT 
GATGAATAG