MEGF8 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	MEGF8
Entrez Gene ID	101123065
Full Name	multiple EGF like domains 8
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015089932.1	XP_014945418.1	multiple epidermal growth factor-like domains protein 8 isoform X2
XM_015100220.1	XP_014955706.1	LOW QUALITY PROTEIN: multiple epidermal growth factor-like domains protein 8 isoform X1
XM_027978350.1	XP_027834151.1	multiple epidermal growth factor-like domains protein 8

Pathways from BioSystems
Gene Ontology

The following MEGF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEGF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv33956	XM_015089932.1 Latest version!	Ovis aries musimon multiple EGF-like-domains 8 (MEGF8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSm52611	XM_015100220.1 Latest version!	Ovis aries multiple EGF-like-domains 8 (MEGF8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSm64515	XM_027978350.1 Latest version!	Ovis aries multiple EGF like domains 8 (MEGF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEGF8 ( XM_015089932.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014945418.1 Ovis aries musimon multiple EGF-like-domains 8 (MEGF8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv33956
Clone ID Related Accession (Same CDS sequence)	XM_015089932.1
Accession Version	XM_015089932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	multiple epidermal growth factor-like domains protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943008.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTATAT CATTGGCCGT GCTGGGGCCA 
CTATCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTACG ACTACCTGTT TGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACGCG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTA 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCCG GCGGCTGCCG CAGCCACGGA CAGTGCCGGC CCCCGGGGGT GTGTGTCTGC 
GAGCCGGGCT GGGGGGGCCC CGACTGCGGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CACGGCACCT GCGCCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGT 
GCCTGTGACC TGCACTTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACCGGGG 
CTGCTGGCTG TTTTCGGAGG ACAGGACCTC AACCGGGCGC TGGGGGACCT CGTTCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGTACCCTCA CCAGCGACAT CTGGGCTTTT AGCCCAATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA CGCAGCAGCC 
CTTGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACAGC ACCAGTGGGG GCTACTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCTGCCACC GGACATTCCA TGGTGTTCCA CGCCCCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA CGTGGATCGG CGTGTATGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCAGG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCCGAGCT TGCCCCTCCA 
GGAACTCCGG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GCGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCCCG TGGGGACTTG 
ATGGCCTACA AAGTACCCCC CTTTGTGTTC CAGGCTCCTG CTCCAGACTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGCTGGTGT 
CAGGGGGCCT GCCAAGCTGC CCCGCCTCCT GGGACTCCCT CGGGGGCTTG TCTGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCGGGG ACCCGGCGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGAGCAGGC CCGCTGCCGT GGGGAGCAGA TCTCTGGCAC CGTGGGCTGG 
TGGGGGCCCG CTCCTGTCTT TGTGACCTCC CTGGAGGCCT GTGTCACCCA GAGCTTCCTG 
CCTGGCCTGC ACCTGCTCAC CTTCCAGCAG CCACCCAACG CCTCCCAGCC TGACAAGGTC 
TTGATCGTCC GCAGCACGAC CATCACCCTG ACGCCGAGCT CAGAGACCGA TGTGTCCCTG 
GTCTACCGTG GCTTCATCCA CCCACTGCTG CCGGGAGGCC CTGGGGGGTC TGGGGCTGAG 
GATGTGGCCG TGTGGGCCCG GGCGCAGCGC CTGCATGTGC TGGCCCGGAT GGCCCGGGGC 
CCAGATGCAG AAAACATGGA GGAGGTGGGG CGCTGGGTGG CACAGCAGGA GAAGGAGACG 
AGGCGGCTGC AACGCCCAGG GTCAGCTCGC CTCTTCCCAC TGCCCGGCCG GGGCCACAAG 
TATGCAGTAG AGATCCGGGG CCAGCTCAAT GGCTCAGCAG GCCCCGGGCA CAGCGAGCTC 
ACCCTGCTGT GGGACCGGAC TGGCGTGCCA GGTGGCAGCG AGATCTCCTT CTTCTTCCTG 
GAGCCCTACC GCTCGTCAGC CTGTGCCTCC TACTCCTCCT GCCTGGGTTG CCTGGCAGAC 
CAGGGCTGCG GCTGGTGCCT GGCCAGTGCC ACCTGCCACC TGCGCCAGAG CGGCGCCCAC 
TGCGGGGACG CTGGGGCCGG CGGGTCCCTG CTCGTGCTGG TGCCTGCCCT CTGCCCGCTC 
TGCGAGGAGC ATCGCGACTG CCACGCCTGC ACCCAGGACC CCTTCTGTGA GTGGCATCAG 
AGCACCAGCC GCAAGGGGGA CGCGGCGTGC AGCCGGCGCG GCCGGGGTCG GGGCGCCCTG 
AAGAGTCCGG AAGAGTGCCC CCCACTCTGC AGCCAGCGGC TGACCTGTGA CGACTGCCTG 
GCCAACTCGA GCCAGTGCGC CTGGTGCCAG TCCACCCGCA CCTGCTTCCT GTTCGCCGCC 
TACCTGGCCC GGTACCCGCA CGGGGGCTGC CGAGGCTGGG ACGACAGCGT GCACTCGGAG 
CCTCGGTGCC GGAGCTGTGA CCGATTCCAG AGCTGTCACG AGTGTCTGCA GAGCCACGAG 
TGTGGCTGGT GTGGAAACGA GGACAACCCC ACGCTGGGCC GTTGCCTCCA GGGGGACTTC 
TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCATATGCC CGCTGTCCAG ATGTGGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTATGAATGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGTGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCGG ACCTGCCCCC TCCGACTCCT GCCCCCGGCC CCCCTGCGCC CCGCTGTTCA 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAC 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCCGG CAGCTTCGGG 
AACGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ATGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GCCTCCTGCC AGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTACAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAC 
GTCCTGGCTT TTGATGGATT CCCACGCTTC CTGGACACTG GTGTTGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTCTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTTCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAACGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCTGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGGT GGACTCTGCC GGTGTCCCCA AGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTT TGTGCTGAGG GCTTCGGGGG TCCTGACTGT 
GCCACAAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCTGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACGCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGCCT CACAGCTGGG GGCGTCTCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCTGTC 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAACG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGTGGGAC GCCCCCCACA GGTCTCTATG GTCACTCTGC TGTGTATCAC 
GAGGCCACCG ACTCCCTCTA CGTGTTTGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCATCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCATGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGATTTCA GCAGCGACGT GCTGCTTTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCT GACCTCATCG GCCCAGCCTC CGTGGGCCCG 
CCAATGGAAG AGTCTGTGGC TCACGCTGTG GCCGCGGTAG GGGGTCGCCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCT TGTCCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC TGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT 
GGGGCCTGCC TGTCCGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGTGC CCCCTGCTCC 
CCTATGCCCC ACTCCCCTGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCG CTGCCCCCCG CTGTAAGTGG 
TGCACCAACT GCCCCGAGGG CGCCTGCATC GGGCGCAATG GGTCCTGCAC CTCAGAGAAC 
GACTGCCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCTGA GGCCGTGTGC 
GGGGCGGCCG ACTGCGCGCA GTGCACCAGG GAGGGCAAGT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CGCTGCTGAA TGTGTCCCCG ATGCCGGTGG AGTCATCACC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TACCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGCGCCCTCT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGC CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CTTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ATGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTGTGCC GCCCGGTGTG CGCCCAGGGT TGCGTGAATG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTTGGC CGCAACTGCT CCACGGAGTG TCGCTGCAAC 
CGTCACAGTG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAACCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCACTGTTCG TGGGTTCAGC CGTGGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGCAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCA GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACAGTGGG TGACAGAGGG TCCCAGTGAA GATGAGGCTG TGTGTGTGCA CTGCCAGAAT 
AACAGCTACG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTTCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACGTGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCT GTGGAGCAGG AGTACTGCCT GGACCCCACG 
TCCCAGACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGCCCCGG CCGCACGGTT 
CTTTTTGGAG TGCAGCCCAA GTTCACCAAT GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA TGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCTGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CGGCTCCCCC ACCCCCCCCA 
CCCCCTGCTG ACGGTGGTCC CCGGGGACCT GGGGATCTGG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTA CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
TTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTCTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC CGTCTTTTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACCGTCTG CTTCCCGCCG GACCCTGCTG CCCCAGCCCC TGCCTGGAAG 
CCTGCCGGGC TCCCGCCACC CACCTTCCGC CGCTCTGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACGGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGCG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGTG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAGTA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCACGGG GCTGGTGCGG GCCGGAAGGG GCTGCTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014945418.1
CDS	775..9144
Translation	MALGRALAVALAISLAVLGPLSPGAQAGDCKGQRQVLREAPGFVTDGAGNYSVNGNCEWLIEAPSSQHRILLDFLFLDTECTYDYLFVYDGDSPRGPLLASLSGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCRSHGQCRPPGVCVCEPGWGGPDCGLQECSAYCGSHGTCASPLGPCRCEPGFLGRACDLHLWENQGAGWWHNVSAGDPAFSARIGAAGAFLSPPGLLAVFGGQDLNRALGDLVLYNFSANTWERWDLSPAPAARHSHVAVAWAGSLVLMGGELADGTLTSDIWAFSPMGGGRWELLAPPASSPSGPPGLAGHAAALVDNTWLYVSGGRTQHDLFSSGLFRFRLDSTSGGYWEQVMPAGGRPPAATGHSMVFHAPSRALLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGREGLQGPRERAFHTASVLGNYMLVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAAPPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACLAASCLGLSRLLGDCQACLAFSSSAAPPRGPGALGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPGLHLLTFQQPPNASQPDKVLIVRSTTITLTPSSETDVSLVYRGFIHPLLPGGPGGSGAEDVAVWARAQRLHVLARMARGPDAENMEEVGRWVAQQEKETRRLQRPGSARLFPLPGRGHKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSACASYSSCLGCLADQGCGWCLASATCHLRQSGAHCGDAGAGGSLLVLVPALCPLCEEHRDCHACTQDPFCEWHQSTSRKGDAACSRRGRGRGALKSPEECPPLCSQRLTCDDCLANSSQCAWCQSTRTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCDRFQSCHECLQSHECGWCGNEDNPTLGRCLQGDFSGPLSGGNCSLWVGEGLGLSVALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTYCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLPPPTPAPGPPAPRCSRDCGCNFHSHCHKRGPGFCDECQDWTWGEHCERCRPGSFGNATGSGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRFGGLLPAGGGAARAGPGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGVPGGGLCRCPQGWAGPHCRMAVCPENCNAHNGAGTCNQSLGVCVCAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGVSRDFWVLNLTTLQWRQEKAPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDDFSSDVLLYQVNCNTWLLPDLIGPASVGPPMEESVAHAVAAVGGRLFISGGFGGVALGRLLALSLPPDPCRLLPSPEACNQTGACTWCHGACLSGDQAHRLGCGGAPCSPMPHSPEECRRLRTCSECLARHPRTLQPGDGEAAAPRCKWCTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAVCGAADCAQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALCLRRPHCGWCTWGAQDGGGRCLEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKAGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSDCAGVGARDHCLLCHNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEIARREPEKYSLDPDEIEQWVTEGPSEDEAVCVHCQNNSYGDKCESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQEYCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPAPPPPPPPADGGPRGPGDLGGPGAGGGPATPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRLVITYPHEHHTLKSSRFYLLLLGVGDPSGPSANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDHRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPAWKPAGLPPPTFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL

Target ORF information:

RefSeq Version	XM_015089932.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon multiple EGF-like-domains 8 (MEGF8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015089932.1

ORF Insert Sequence:

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTATAT CATTGGCCGT GCTGGGGCCA 
CTATCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTACG ACTACCTGTT TGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACGCG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTA 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCCG GCGGCTGCCG CAGCCACGGA CAGTGCCGGC CCCCGGGGGT GTGTGTCTGC 
GAGCCGGGCT GGGGGGGCCC CGACTGCGGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CACGGCACCT GCGCCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGT 
GCCTGTGACC TGCACTTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACCGGGG 
CTGCTGGCTG TTTTCGGAGG ACAGGACCTC AACCGGGCGC TGGGGGACCT CGTTCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGTACCCTCA CCAGCGACAT CTGGGCTTTT AGCCCAATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA CGCAGCAGCC 
CTTGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACAGC ACCAGTGGGG GCTACTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCTGCCACC GGACATTCCA TGGTGTTCCA CGCCCCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA CGTGGATCGG CGTGTATGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCAGG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCCGAGCT TGCCCCTCCA 
GGAACTCCGG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GCGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCCCG TGGGGACTTG 
ATGGCCTACA AAGTACCCCC CTTTGTGTTC CAGGCTCCTG CTCCAGACTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGCTGGTGT 
CAGGGGGCCT GCCAAGCTGC CCCGCCTCCT GGGACTCCCT CGGGGGCTTG TCTGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCGGGG ACCCGGCGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGAGCAGGC CCGCTGCCGT GGGGAGCAGA TCTCTGGCAC CGTGGGCTGG 
TGGGGGCCCG CTCCTGTCTT TGTGACCTCC CTGGAGGCCT GTGTCACCCA GAGCTTCCTG 
CCTGGCCTGC ACCTGCTCAC CTTCCAGCAG CCACCCAACG CCTCCCAGCC TGACAAGGTC 
TTGATCGTCC GCAGCACGAC CATCACCCTG ACGCCGAGCT CAGAGACCGA TGTGTCCCTG 
GTCTACCGTG GCTTCATCCA CCCACTGCTG CCGGGAGGCC CTGGGGGGTC TGGGGCTGAG 
GATGTGGCCG TGTGGGCCCG GGCGCAGCGC CTGCATGTGC TGGCCCGGAT GGCCCGGGGC 
CCAGATGCAG AAAACATGGA GGAGGTGGGG CGCTGGGTGG CACAGCAGGA GAAGGAGACG 
AGGCGGCTGC AACGCCCAGG GTCAGCTCGC CTCTTCCCAC TGCCCGGCCG GGGCCACAAG 
TATGCAGTAG AGATCCGGGG CCAGCTCAAT GGCTCAGCAG GCCCCGGGCA CAGCGAGCTC 
ACCCTGCTGT GGGACCGGAC TGGCGTGCCA GGTGGCAGCG AGATCTCCTT CTTCTTCCTG 
GAGCCCTACC GCTCGTCAGC CTGTGCCTCC TACTCCTCCT GCCTGGGTTG CCTGGCAGAC 
CAGGGCTGCG GCTGGTGCCT GGCCAGTGCC ACCTGCCACC TGCGCCAGAG CGGCGCCCAC 
TGCGGGGACG CTGGGGCCGG CGGGTCCCTG CTCGTGCTGG TGCCTGCCCT CTGCCCGCTC 
TGCGAGGAGC ATCGCGACTG CCACGCCTGC ACCCAGGACC CCTTCTGTGA GTGGCATCAG 
AGCACCAGCC GCAAGGGGGA CGCGGCGTGC AGCCGGCGCG GCCGGGGTCG GGGCGCCCTG 
AAGAGTCCGG AAGAGTGCCC CCCACTCTGC AGCCAGCGGC TGACCTGTGA CGACTGCCTG 
GCCAACTCGA GCCAGTGCGC CTGGTGCCAG TCCACCCGCA CCTGCTTCCT GTTCGCCGCC 
TACCTGGCCC GGTACCCGCA CGGGGGCTGC CGAGGCTGGG ACGACAGCGT GCACTCGGAG 
CCTCGGTGCC GGAGCTGTGA CCGATTCCAG AGCTGTCACG AGTGTCTGCA GAGCCACGAG 
TGTGGCTGGT GTGGAAACGA GGACAACCCC ACGCTGGGCC GTTGCCTCCA GGGGGACTTC 
TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCATATGCC CGCTGTCCAG ATGTGGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTATGAATGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGTGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCGG ACCTGCCCCC TCCGACTCCT GCCCCCGGCC CCCCTGCGCC CCGCTGTTCA 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAC 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCCGG CAGCTTCGGG 
AACGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ATGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GCCTCCTGCC AGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTACAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAC 
GTCCTGGCTT TTGATGGATT CCCACGCTTC CTGGACACTG GTGTTGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTCTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTTCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAACGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCTGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGGT GGACTCTGCC GGTGTCCCCA AGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTT TGTGCTGAGG GCTTCGGGGG TCCTGACTGT 
GCCACAAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCTGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACGCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGCCT CACAGCTGGG GGCGTCTCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCTGTC 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAACG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGTGGGAC GCCCCCCACA GGTCTCTATG GTCACTCTGC TGTGTATCAC 
GAGGCCACCG ACTCCCTCTA CGTGTTTGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCATCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCATGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGATTTCA GCAGCGACGT GCTGCTTTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCT GACCTCATCG GCCCAGCCTC CGTGGGCCCG 
CCAATGGAAG AGTCTGTGGC TCACGCTGTG GCCGCGGTAG GGGGTCGCCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCT TGTCCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC TGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT 
GGGGCCTGCC TGTCCGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGTGC CCCCTGCTCC 
CCTATGCCCC ACTCCCCTGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCG CTGCCCCCCG CTGTAAGTGG 
TGCACCAACT GCCCCGAGGG CGCCTGCATC GGGCGCAATG GGTCCTGCAC CTCAGAGAAC 
GACTGCCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCTGA GGCCGTGTGC 
GGGGCGGCCG ACTGCGCGCA GTGCACCAGG GAGGGCAAGT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CGCTGCTGAA TGTGTCCCCG ATGCCGGTGG AGTCATCACC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TACCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGCGCCCTCT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGC CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CTTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ATGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTGTGCC GCCCGGTGTG CGCCCAGGGT TGCGTGAATG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTTGGC CGCAACTGCT CCACGGAGTG TCGCTGCAAC 
CGTCACAGTG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAACCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCACTGTTCG TGGGTTCAGC CGTGGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGCAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCA GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACAGTGGG TGACAGAGGG TCCCAGTGAA GATGAGGCTG TGTGTGTGCA CTGCCAGAAT 
AACAGCTACG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTTCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACGTGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCT GTGGAGCAGG AGTACTGCCT GGACCCCACG 
TCCCAGACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGCCCCGG CCGCACGGTT 
CTTTTTGGAG TGCAGCCCAA GTTCACCAAT GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA TGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCTGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CGGCTCCCCC ACCCCCCCCA 
CCCCCTGCTG ACGGTGGTCC CCGGGGACCT GGGGATCTGG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTA CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
TTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTCTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC CGTCTTTTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACCGTCTG CTTCCCGCCG GACCCTGCTG CCCCAGCCCC TGCCTGGAAG 
CCTGCCGGGC TCCCGCCACC CACCTTCCGC CGCTCTGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACGGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGCG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGTG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAGTA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCACGGG GCTGGTGCGG GCCGGAAGGG GCTGCTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF8 ( XM_015100220.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014955706.1 Ovis aries multiple EGF-like-domains 8 (MEGF8), transcript variant X1, mRNA.

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CloneID	OSm52611
Clone ID Related Accession (Same CDS sequence)	XM_015100220.1
Accession Version	XM_015100220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: multiple epidermal growth factor-like domains protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639023.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 4 indels ##RefSeq-Attributes-END##

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTATAT CATTGGCCGT GCTGGGGCCA 
CTATCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTACG ACTACCTGTT TGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACGCG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTA 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCCG GCGGCTGCCG CAGCCACGGA CAGTGCCGGC CCCCGGGGGT GTGTGTCTGC 
GAGCCGGGCT GGGGGGGCCC CGACTGCGGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CACGGCACCT GCGCCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGT 
GCCTGTGACC TGCACTTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACCGGGG 
CTGCTGGCTG TTTTCGGAGG ACAGGACCTC AACCGGGCGC TGGGGGACCT CGTTCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGTACCCTCA CCAGCGACAT CTGGGCTTTT AGCCCAATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA CGCAGCAGCC 
CTTGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACAGC ACCAGTGGGG GCTACTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCTGCCACC GGACATTCCA TGGTGTTCCA CGCCCCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA CGTGGATCGG CGTGTATGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCAGG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCCGAGCT TGCCCCTCCA 
GGAACTCCGG AGGGTGAGTC GCCCCTCTGT TTCTGTCAGG GGCTCCTGAG GGTCACTCAC 
ACTCCCACCA TGATGTGGGA CTTTGGGCAG AAGCCACTCG GTCCTGTTTG GAGCTGGCCT 
CAGGAGGCGT GGCTGCACTG GAGGCAGGTG GGTAACCGTG AAGGTGAGAG GGGGCGTTGC 
GGGGGTGGTC TCACGTCCTT GTGGGGTCTG GGGGACTTGA TGGCCTACAA AGTACCCCCC 
TTTGTGTTCC AGGCTCCTGC TCCAGACTAC CACTTGGACT ATTGCTCCAT GTACACGGAC 
CACAGCGTCT GCTCCCGAGA CCCCGAATGC AGCTGGTGTC AGGGGGCCTG CCAAGCCGCC 
CCGCCTCCTG GGACTCCCTC GGGGGCTTGT CTGGCTGCCA GCTGCCTGGG CCTGAGCCGT 
CTCCTGGGTG ACTGCCAGGG GGCCACCACA CACCGACTTC CCTCTCCTGA CCCTCCTGTC 
CCCACAGAGC AGGCCCGCTG CCGTGGGGAG CAGATCTCTG GCACCGTGGG CTGGTGGGGG 
CCCGCTCCTG TCTTTGTGAC CTCCCTGGAG GCCTGTGTCA CCCAGAGCTT CCTGCCTGGC 
CTGCACCTGC TCACCTTCCA GCAGCCACCC AACGCCTCCC AGCCTGACAA GGTCTTGATC 
GTCCGCAGCA CGACCATCAC CCTGACGCCG AGCTCAGAGA CCGATGTGTC CCTGGTCTAC 
CGTGGCTTCA TCCACCCGCT GCTGCCGGGA GGCCCTGGGG GGTCTGGGGC TGAGGATGTG 
GCCGTGTGGG CCCGGGCGCA GCGCCTGCAT GTGCTGGCCC GGATGGCCCG GGGCCCAGAT 
GCAGAAAACA TGGAGGAGGT GGGGCGCTGG GTGGCACAGC AGGAGAAGGA GACGAGGCGG 
CTGCAACGCC CAGGGTCAGC TCGCCTCTTC CCACTGCCCG GCCGGGGCCA CAAGTATGCA 
GTAGAGATCC GGGGCCAGCT CAATGGCTCA GCAGGCCCCG GGCACAGCGA GCTCACCCTG 
CTGTGGGACC GGACTGGCGT GCCAGGTGGC AGCGAGATCT CCTTCTTCTT CCTGGAGCCC 
TACCGCTCGT CAGCCTGTGC CTCCTACTCC TCCTGCCTGG GTTGCCTGGC AGACCAGGGC 
TGCGGCTGGT GCCTGGCCAG TGCCACCTGC CACCTGCGCC AGAGCGGCGC CCACTGCGGG 
GACGCTGGGG CCGGCGGGTC CCTGCTCGTG CTGGTGCCTG CCCTCTGCCC GCTCTGCGAG 
GAGCATCGCG ACTGCCACGC CTGCACCCAG GACCCCTTCT GTGAGTGGCA TCAGAGCACC 
AGCCGCAAGG GGGACGCGGC GTGCAGCCGG CGCGGCCGGG GTCGGGGCGC CCTGAAGAGT 
CCGGAAGAGT GCCCCCCACT CTGCAGCCAG CGGCTGACCT GTGACGACTG CCTGGCCAAC 
TCGAGCCAGT GCGCCTGGTG CCAGTCCACC CGCACCTGCT TCCTGGTCGC CGCCTACCTG 
GCCCGGTACC CGCACNGGGG CTGCCGAGGC TGGGACGACA GCGTGCACTC GGAGCCTCGG 
TGCCGGAGCT GTGACCGATT CCAGAGCTGT CACGAGTGTC TGCAGAGCCA CGAGTGTGGC 
TGGTGTGGAA ACGAGACAAC CCCACGCTGT TGCCTCCAGG GGGACTTCTC AGGGCCTCTG 
AGTGAGGGGA ACTGCTTGGG GGGGGGCCGG GGGCTGTCTG GGGCCCTCCC CGCCCGCTGG 
GCATATGCCC GCTGTCCAGA TGTGGACGAG TGCCGCCTGG GCCTGGCGCG GTGCCACCTG 
CGGGCTACCT GCCTGAACAC ACCCCTCAGC TATGAATGTC ACTGCCAGAG GGGCTACCAG 
GGCGATGGCG TCACTTACTG CAACCGCACG TGCCTGGAGG ACTGTGGCCA CGGTGTGTGC 
AGTGGCCCCC CAGACTTCAC CTGCGTGTGT GACCTGGGCT GGACATCGGA CCAGCGGGGG 
CCCGGCTTCT GCGACGAGTG CCAGGACTGG ACGTGGGGGG AGCATTGTGA GCGGTGCCGG 
CCCGGCAGCT CCAGGTCCCT CGGTCCCACT GGCTGCCGGC CCTGCCAGTG CAATGGGCAT 
GGGGACCCTC GCCGAGGCCA CTGTGACAAC CTCAGCGGGC TCTGCTTCTG CCAGGACCAC 
ACCGAGGGTG CCCACTGCCA GCTCTGCTCC CCTGGCTACT ACGGGGACCC TCGGGCAGGT 
GGCTCCTGCT TCCGGGAGTG TGGGGGTCGT GCCCTCCTCA CCAACGTGTC CTCAGTGGCA 
CTGGGCTCAC GCCGGTTTGG GGGCCTCCTG CCAGCAGGTG GCGGGGCAGC AAGAGCGGGG 
CCTGGCCTGT CCTACTGTGT GTGGGTTGTC TCGGCCACTG AGGTGCTACA GCCCTGTGCC 
CCCGGGACCC TCTGTCCCCC GCTTACCCTC ACCTTCTCCC CTGACAGCAG CACCCCGTGC 
ACGCTGAGCT ACGTCCTGGC TTTTGATGGA TTCCCACGCT TCCTGGACAC TGGTGTTGTC 
CAGTCGGACC GTAGCCTCAT CGCTGCGTTC TGCGGCCAGC GGCGGGACCG GCCCCTCACT 
GTGCAGGCCC TGTCTGGGCT GCTGGTACTT CACTGGGAGG CCAATGGCTC CTCATCCTGG 
GGCTTCAACG CCTCCGTGGG CTCTGCCCGC TGTGGGTCAG GGGGCCCTGG GAGCTGCCCT 
GTCCCCCAGG AGTGTGTGCC CCAGGATGGG GTCCCAGGTG GTGGACTCTG CCGGTGTCCC 
CAAGGCTGGG CTGGCCCGCA CTGCCGCATG GCTGTGTGTC CTGAGAACTG TAATGCCCAC 
AACGGGGCGG GGACGTGTAA TCAGAGCCTG GGCGTGTGCG TTTGTGCTGA GGGCTTCGGG 
GGTCCTGACT GTGCCACAAA GCTGGATGGT GGGCAGCTGG TCTGGGAGAC CCTCATGGAC 
AGCCGCCTCT CAGCTGACAC TGCCAGCCGC TTCCTGCACC GCCTGGGGCA CACGATGGTG 
GAGGGACCTG ATGCCACCTT GTGGATGTTT GGGGGCCTGG GCCTGCCCCA GGGGCTGCTG 
GGAAACCTGT ATAGGTACTC AGTGAGTGAA CGGCGGTGGA CACAGATGTT GGCAGGAGCT 
GAGGATGGGG GCCCAGGCCC CTCCCCTCGC TCTTTCCACG CTGCTGCCTA CGTGCCTGCT 
GGCCGTGGGG CCATGTACCT GCTGGGGGGC TGGCGGCAGG AGAAGGCCCC TCAGACTGTG 
GAGCTGCCAG CTGTCGCTGG TCACACGCTT ACTGCCCGCC GAGGCCTATC TCTGCTCCTG 
GTGGGTGGTT ACTCCCCGGA AAATGGCTTC AACCAGCAGC TGCTTGAGTA CCAGCTGGCG 
ACTGGCACCT GGGTGTCCGG GGCTCAGAGT GGGACGCCCC CCACAGGTCT CTATGGTCAC 
TCTGCTGTGT ATCACGAGGC CACCGACTCC CTCTACGTGT TTGGGGGTTT CCGCTTCCAT 
GTGGAGCTGG CAGCCCCGTC CCCTGAGCTC TACTCCCTGC ACTGCCCTGA CCGCACCTGG 
AGCCTGCTGG CCCCTTCTCA GGGAGCAAAG CCCCGGCCCC GACTCTTCCA TGCCTCGGCC 
TTGCTAGGGG ACACCATGGT GGTCCTCGGG GGGCGCTCAG ACCCCGACGA GTTCAGCAGC 
GACGTGCTGC TTTACCAGGT CAACTGCAAC ACCTGGCTCC TGCCTGACCT CATCGGCCCA 
GCCCCCGTGG GCCCGCCAAT GGAAGAGTCT GTGGCTCACG CTGTGGCCGC GGTAGGGGGT 
CGCCTGTTTA TCTCAGGAGG CTTTGGGGGA GTGGCCCTGG GCCGCCTGCT GGCCTTGTCC 
CTGCCCCCTG ACCCCTGCCG CCTGCTGCCC TCGCCTGAAG CTTGTAACCA GACTGGGGCC 
TGTACCTGGT GCCATGGGGC CTGCCTGTCC GGGGACCAGG CCCACAGACT GGGCTGTGGC 
GGCGCCCCCT GCTCCCCTAT GCCCCACTCC CCTGAGGAAT GTCGACGTCT GCGGACCTGC 
AGCGAGTGCC TGGCCCGCCA TCCCCGGACC CTGCAGCCTG GAGATGGAGA GGTGAGTGAT 
GGGGTGGGGG CTTTGGGGAA CGTGTTTCAG GCACCCGGGG CTCTTCACCT GGATCAGGTG 
GCAGCAGCTG AGACCGGGGG GGTGCTGGGG GCCTTGACGC CTCTCCGAGC CGCAGACAAC 
GACTGCCGCA TAAACAAGCG CGTGGTCTTC TGGGCCGGGA ACTGCTCTGA GGCCGTGTGC 
GGGGCGGCCG ACTGCGCGCA GTGCACCAGG GAGGGCAAGT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CGCTGCTGAA TGTGTCCCCG ATGCCGGTGG AGTCATCACC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TACCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGGCCC AGGATGGGGG CGGCCGCTGC 
CTGGAGGGTG GACTCAGTGG CCCCCGTGAC GGGCTGACGT GTGGGCGACC TGGGGCCTCT 
TGGGCCTTTC TTTCCTGCCC CCCGGAGGAC GAATGTGCCA ACGGGCACCA TGACTGCAAT 
GAGACCCAGA ACTGCCACGA CCGGCCCCAC GGTTACGAGT GCAGCTGCAA GGCAGGCTAC 
ACCATGGACA ACGTGACGGG GCTGTGCCGC CCGGTGTGCG CCCAGGGTTG CGTGAATGGC 
TCGTGTGTGG AGCCCGACCA CTGCCGCTGC CACTTCGGCT TCGTTGGCCG CAACTGCTCC 
ACGGAGTGTC GCTGCAACCG TCACAGTGAC TGCGCCGGCG TGGGGGCCCG CGACCACTGC 
CTGCTCTGCC ACAACCACAC CAAGGGCAGC CACTGTGAGC AGTGCCTCCC ACTGTTCGTG 
GGTTCAGCCG TGGGGGGAGG GACCTGCCGG CCCTGCCACG CCTTTTGTCG CGGCAACAGC 
CACGTCTGCG TCTCCAGGAA GGAGCTTGAG ATAGCCAGGA GGGAGCCAGA GAAGTACTCG 
CTGGATCCAG ATGAGATTGA ACAGTGGGTG ACAGAGGGTC CCAGTGAAGA TGAGGCTGTG 
TGTGTGCACT GCCAGAATAA CAGCTACGGG GATAAGTGTG AGAGCTGCCT CCACGGCTAC 
TTTCTCCTCG ACAGGAAATG CATCAGATGC CAGTGTAATG GCCACGCGGA CACGTGTAAT 
GAGCAGGATG GGACAGGCTG CCCATGTCAG AACAACACAG AGACAGGCAC GTGCCAGGGC 
AGCTCCCCCA GTGACCGCCG AGACTGCTAC AAATACCAGT GTGCCAAGTG CCGAGAGTCA 
TTCCACGGGA GCCCGCTAGG GGGCCAGCAG TGCTACCGCC TCATCTCTGT GGAGCAGGAG 
TACTGCCTGG ACCCCACGTC CCAGACCAAC TGTTTCCACG AGCCCAAGCG CCGGGCTCTG 
GGCCCCGGCC GCACGGTTCT TTTTGGAGTG CAGCCCAAGT TCACCAATGT GGACATCCGC 
CTGACGCTGG ACGTGACCTT TGGCGCCGTG GACCTCTATG TCTCCACCTC CTATGACACC 
TTCGTGGTCC GCGTGGCTCC TGACACTGGT GTCCACACCG TGCATATCCA GCCGCCACCG 
CCCCCCCCAC CCCCCCCACC CCCCCCCTGG GGACCTGGGG ATCGGGGGGG ACCTGGGGCT 
GGAGGTGGGC CGGCTACCCC AGCAGAGCCG CGAGTACGGG AGGTCTGGCC ACGGGGCCTG 
ATCACCTACG TGACAGTGAC AGAGCCGTCG GCGGTGCTGG TGGTCCGTGG CGTGAGGGAC 
CGGCTGGTCA TCACCTACCC GCACGAGCAC CACACCCTCA AGTCCAGCCG CTTCTACCTG 
CTCCTGTTGG GTGTTGGAGA CCCAAGTGGG CCCAGTGCCA ACGGCTCAGC TGACTCGCAG 
GGCTTGCTCT TCTTCCGGCA GGACCAGGCC CACATTGACC TGTTTGTCTT CTTCTCCGTC 
TTTTTCTCCT GCTTCTTCCT CTTCCTCTCG CTCTGTGTGC TGCTCTGGAA GGCCAAGCAG 
GCCCTGGACC ACCGGCAGGA ACAGCGCCGG CACCTGCAGG AGATGACCAA GATGGCCAGC 
CGCCCCTTCG CCAAGGTCAC CGTCTGCTTC CCGCCGGACC CTGCTGCCCC AGCCCCTGCC 
TGGAAGCCTG CCGGGCTCCC GCCACCCACC TTCCGCCGCT CTGAGCCCTT CTTGGCACCC 
TTGCTGCTGA CGGGGGCCGG CGGGCCCTGG GGACCTGTAG GAGGCGGCTG CTGCCCACCG 
GCCATCCCCA CCACCACTGC CGGCCTGCGA GCCGGGCCTA TCACCCTTGA GCCCACCGAG 
GATGGCATGG CCGGCGTGGC CACACTGCTG CTCCAACTGC CTGGTGGGCC CCACGCACCC 
AACGGCGCCT GCCTGGGGTC AGCGCTCGTC ACACTGCGGC ACCGGCTGCA CGAGTACTGC 
GGCGGCGGCG GGGGCGCTGG GGGCAGTGGG CACGGGGCTG GTGCGGGCCG GAAGGGGCTG 
CTGAGCCAGG ACAACCTCAC CAGCATGTCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014955706.1
CDS	451..8646
Translation	MALGRALAVALAISLAVLGPLSPGAQAGDCKGQRQVLREAPGFVTDGAGNYSVNGNCEWLIEAPSSQHRILLDFLFLDTECTYDYLFVYDGDSPRGPLLASLSGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCRSHGQCRPPGVCVCEPGWGGPDCGLQECSAYCGSHGTCASPLGPCRCEPGFLGRACDLHLWENQGAGWWHNVSAGDPAFSARIGAAGAFLSPPGLLAVFGGQDLNRALGDLVLYNFSANTWERWDLSPAPAARHSHVAVAWAGSLVLMGGELADGTLTSDIWAFSPMGGGRWELLAPPASSPSGPPGLAGHAAALVDNTWLYVSGGRTQHDLFSSGLFRFRLDSTSGGYWEQVMPAGGRPPAATGHSMVFHAPSRALLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGREGLQGPRERAFHTASVLGNYMLVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGESPLCFCQGLLRVTHTPTMMWDFGQKPLGPVWSWPQEAWLHWRQVGNREGERGRCGGGLTSLWGLGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACLAASCLGLSRLLGDCQGATTHRLPSPDPPVPTEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPGLHLLTFQQPPNASQPDKVLIVRSTTITLTPSSETDVSLVYRGFIHPLLPGGPGGSGAEDVAVWARAQRLHVLARMARGPDAENMEEVGRWVAQQEKETRRLQRPGSARLFPLPGRGHKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSACASYSSCLGCLADQGCGWCLASATCHLRQSGAHCGDAGAGGSLLVLVPALCPLCEEHRDCHACTQDPFCEWHQSTSRKGDAACSRRGRGRGALKSPEECPPLCSQRLTCDDCLANSSQCAWCQSTRTCFLVAAYLARYPHnullGCRGWDDSVHSEPRCRSCDRFQSCHECLQSHECGWCGNETTPRCCLQGDFSGPLSEGNCLGGGRGLSGALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTYCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDQRGPGFCDECQDWTWGEHCERCRPGSSRSLGPTGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRFGGLLPAGGGAARAGPGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGVPGGGLCRCPQGWAGPHCRMAVCPENCNAHNGAGTCNQSLGVCVCAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGWRQEKAPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPDLIGPAPVGPPMEESVAHAVAAVGGRLFISGGFGGVALGRLLALSLPPDPCRLLPSPEACNQTGACTWCHGACLSGDQAHRLGCGGAPCSPMPHSPEECRRLRTCSECLARHPRTLQPGDGEVSDGVGALGNVFQAPGALHLDQVAAAETGGVLGALTPLRAADNDCRINKRVVFWAGNCSEAVCGAADCAQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGAQDGGGRCLEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKAGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSDCAGVGARDHCLLCHNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEIARREPEKYSLDPDEIEQWVTEGPSEDEAVCVHCQNNSYGDKCESCLHGYFLLDRKCIRCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQEYCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPPPPPWGPGDRGGPGAGGGPATPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRLVITYPHEHHTLKSSRFYLLLLGVGDPSGPSANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDHRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPAWKPAGLPPPTFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL

Target ORF information:

RefSeq Version	XM_015100220.1
Organism	Ovis aries(sheep)
Definition	Ovis aries multiple EGF-like-domains 8 (MEGF8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015100220.1

ORF Insert Sequence:

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTATAT CATTGGCCGT GCTGGGGCCA 
CTATCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTACG ACTACCTGTT TGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACGCG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTA 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCCG GCGGCTGCCG CAGCCACGGA CAGTGCCGGC CCCCGGGGGT GTGTGTCTGC 
GAGCCGGGCT GGGGGGGCCC CGACTGCGGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CACGGCACCT GCGCCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGT 
GCCTGTGACC TGCACTTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACCGGGG 
CTGCTGGCTG TTTTCGGAGG ACAGGACCTC AACCGGGCGC TGGGGGACCT CGTTCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGTACCCTCA CCAGCGACAT CTGGGCTTTT AGCCCAATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA CGCAGCAGCC 
CTTGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCGACAGC ACCAGTGGGG GCTACTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCTGCCACC GGACATTCCA TGGTGTTCCA CGCCCCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA CGTGGATCGG CGTGTATGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCAGG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCCGAGCT TGCCCCTCCA 
GGAACTCCGG AGGGTGAGTC GCCCCTCTGT TTCTGTCAGG GGCTCCTGAG GGTCACTCAC 
ACTCCCACCA TGATGTGGGA CTTTGGGCAG AAGCCACTCG GTCCTGTTTG GAGCTGGCCT 
CAGGAGGCGT GGCTGCACTG GAGGCAGGTG GGTAACCGTG AAGGTGAGAG GGGGCGTTGC 
GGGGGTGGTC TCACGTCCTT GTGGGGTCTG GGGGACTTGA TGGCCTACAA AGTACCCCCC 
TTTGTGTTCC AGGCTCCTGC TCCAGACTAC CACTTGGACT ATTGCTCCAT GTACACGGAC 
CACAGCGTCT GCTCCCGAGA CCCCGAATGC AGCTGGTGTC AGGGGGCCTG CCAAGCCGCC 
CCGCCTCCTG GGACTCCCTC GGGGGCTTGT CTGGCTGCCA GCTGCCTGGG CCTGAGCCGT 
CTCCTGGGTG ACTGCCAGGG GGCCACCACA CACCGACTTC CCTCTCCTGA CCCTCCTGTC 
CCCACAGAGC AGGCCCGCTG CCGTGGGGAG CAGATCTCTG GCACCGTGGG CTGGTGGGGG 
CCCGCTCCTG TCTTTGTGAC CTCCCTGGAG GCCTGTGTCA CCCAGAGCTT CCTGCCTGGC 
CTGCACCTGC TCACCTTCCA GCAGCCACCC AACGCCTCCC AGCCTGACAA GGTCTTGATC 
GTCCGCAGCA CGACCATCAC CCTGACGCCG AGCTCAGAGA CCGATGTGTC CCTGGTCTAC 
CGTGGCTTCA TCCACCCGCT GCTGCCGGGA GGCCCTGGGG GGTCTGGGGC TGAGGATGTG 
GCCGTGTGGG CCCGGGCGCA GCGCCTGCAT GTGCTGGCCC GGATGGCCCG GGGCCCAGAT 
GCAGAAAACA TGGAGGAGGT GGGGCGCTGG GTGGCACAGC AGGAGAAGGA GACGAGGCGG 
CTGCAACGCC CAGGGTCAGC TCGCCTCTTC CCACTGCCCG GCCGGGGCCA CAAGTATGCA 
GTAGAGATCC GGGGCCAGCT CAATGGCTCA GCAGGCCCCG GGCACAGCGA GCTCACCCTG 
CTGTGGGACC GGACTGGCGT GCCAGGTGGC AGCGAGATCT CCTTCTTCTT CCTGGAGCCC 
TACCGCTCGT CAGCCTGTGC CTCCTACTCC TCCTGCCTGG GTTGCCTGGC AGACCAGGGC 
TGCGGCTGGT GCCTGGCCAG TGCCACCTGC CACCTGCGCC AGAGCGGCGC CCACTGCGGG 
GACGCTGGGG CCGGCGGGTC CCTGCTCGTG CTGGTGCCTG CCCTCTGCCC GCTCTGCGAG 
GAGCATCGCG ACTGCCACGC CTGCACCCAG GACCCCTTCT GTGAGTGGCA TCAGAGCACC 
AGCCGCAAGG GGGACGCGGC GTGCAGCCGG CGCGGCCGGG GTCGGGGCGC CCTGAAGAGT 
CCGGAAGAGT GCCCCCCACT CTGCAGCCAG CGGCTGACCT GTGACGACTG CCTGGCCAAC 
TCGAGCCAGT GCGCCTGGTG CCAGTCCACC CGCACCTGCT TCCTGGTCGC CGCCTACCTG 
GCCCGGTACC CGCACNGGGG CTGCCGAGGC TGGGACGACA GCGTGCACTC GGAGCCTCGG 
TGCCGGAGCT GTGACCGATT CCAGAGCTGT CACGAGTGTC TGCAGAGCCA CGAGTGTGGC 
TGGTGTGGAA ACGAGACAAC CCCACGCTGT TGCCTCCAGG GGGACTTCTC AGGGCCTCTG 
AGTGAGGGGA ACTGCTTGGG GGGGGGCCGG GGGCTGTCTG GGGCCCTCCC CGCCCGCTGG 
GCATATGCCC GCTGTCCAGA TGTGGACGAG TGCCGCCTGG GCCTGGCGCG GTGCCACCTG 
CGGGCTACCT GCCTGAACAC ACCCCTCAGC TATGAATGTC ACTGCCAGAG GGGCTACCAG 
GGCGATGGCG TCACTTACTG CAACCGCACG TGCCTGGAGG ACTGTGGCCA CGGTGTGTGC 
AGTGGCCCCC CAGACTTCAC CTGCGTGTGT GACCTGGGCT GGACATCGGA CCAGCGGGGG 
CCCGGCTTCT GCGACGAGTG CCAGGACTGG ACGTGGGGGG AGCATTGTGA GCGGTGCCGG 
CCCGGCAGCT CCAGGTCCCT CGGTCCCACT GGCTGCCGGC CCTGCCAGTG CAATGGGCAT 
GGGGACCCTC GCCGAGGCCA CTGTGACAAC CTCAGCGGGC TCTGCTTCTG CCAGGACCAC 
ACCGAGGGTG CCCACTGCCA GCTCTGCTCC CCTGGCTACT ACGGGGACCC TCGGGCAGGT 
GGCTCCTGCT TCCGGGAGTG TGGGGGTCGT GCCCTCCTCA CCAACGTGTC CTCAGTGGCA 
CTGGGCTCAC GCCGGTTTGG GGGCCTCCTG CCAGCAGGTG GCGGGGCAGC AAGAGCGGGG 
CCTGGCCTGT CCTACTGTGT GTGGGTTGTC TCGGCCACTG AGGTGCTACA GCCCTGTGCC 
CCCGGGACCC TCTGTCCCCC GCTTACCCTC ACCTTCTCCC CTGACAGCAG CACCCCGTGC 
ACGCTGAGCT ACGTCCTGGC TTTTGATGGA TTCCCACGCT TCCTGGACAC TGGTGTTGTC 
CAGTCGGACC GTAGCCTCAT CGCTGCGTTC TGCGGCCAGC GGCGGGACCG GCCCCTCACT 
GTGCAGGCCC TGTCTGGGCT GCTGGTACTT CACTGGGAGG CCAATGGCTC CTCATCCTGG 
GGCTTCAACG CCTCCGTGGG CTCTGCCCGC TGTGGGTCAG GGGGCCCTGG GAGCTGCCCT 
GTCCCCCAGG AGTGTGTGCC CCAGGATGGG GTCCCAGGTG GTGGACTCTG CCGGTGTCCC 
CAAGGCTGGG CTGGCCCGCA CTGCCGCATG GCTGTGTGTC CTGAGAACTG TAATGCCCAC 
AACGGGGCGG GGACGTGTAA TCAGAGCCTG GGCGTGTGCG TTTGTGCTGA GGGCTTCGGG 
GGTCCTGACT GTGCCACAAA GCTGGATGGT GGGCAGCTGG TCTGGGAGAC CCTCATGGAC 
AGCCGCCTCT CAGCTGACAC TGCCAGCCGC TTCCTGCACC GCCTGGGGCA CACGATGGTG 
GAGGGACCTG ATGCCACCTT GTGGATGTTT GGGGGCCTGG GCCTGCCCCA GGGGCTGCTG 
GGAAACCTGT ATAGGTACTC AGTGAGTGAA CGGCGGTGGA CACAGATGTT GGCAGGAGCT 
GAGGATGGGG GCCCAGGCCC CTCCCCTCGC TCTTTCCACG CTGCTGCCTA CGTGCCTGCT 
GGCCGTGGGG CCATGTACCT GCTGGGGGGC TGGCGGCAGG AGAAGGCCCC TCAGACTGTG 
GAGCTGCCAG CTGTCGCTGG TCACACGCTT ACTGCCCGCC GAGGCCTATC TCTGCTCCTG 
GTGGGTGGTT ACTCCCCGGA AAATGGCTTC AACCAGCAGC TGCTTGAGTA CCAGCTGGCG 
ACTGGCACCT GGGTGTCCGG GGCTCAGAGT GGGACGCCCC CCACAGGTCT CTATGGTCAC 
TCTGCTGTGT ATCACGAGGC CACCGACTCC CTCTACGTGT TTGGGGGTTT CCGCTTCCAT 
GTGGAGCTGG CAGCCCCGTC CCCTGAGCTC TACTCCCTGC ACTGCCCTGA CCGCACCTGG 
AGCCTGCTGG CCCCTTCTCA GGGAGCAAAG CCCCGGCCCC GACTCTTCCA TGCCTCGGCC 
TTGCTAGGGG ACACCATGGT GGTCCTCGGG GGGCGCTCAG ACCCCGACGA GTTCAGCAGC 
GACGTGCTGC TTTACCAGGT CAACTGCAAC ACCTGGCTCC TGCCTGACCT CATCGGCCCA 
GCCCCCGTGG GCCCGCCAAT GGAAGAGTCT GTGGCTCACG CTGTGGCCGC GGTAGGGGGT 
CGCCTGTTTA TCTCAGGAGG CTTTGGGGGA GTGGCCCTGG GCCGCCTGCT GGCCTTGTCC 
CTGCCCCCTG ACCCCTGCCG CCTGCTGCCC TCGCCTGAAG CTTGTAACCA GACTGGGGCC 
TGTACCTGGT GCCATGGGGC CTGCCTGTCC GGGGACCAGG CCCACAGACT GGGCTGTGGC 
GGCGCCCCCT GCTCCCCTAT GCCCCACTCC CCTGAGGAAT GTCGACGTCT GCGGACCTGC 
AGCGAGTGCC TGGCCCGCCA TCCCCGGACC CTGCAGCCTG GAGATGGAGA GGTGAGTGAT 
GGGGTGGGGG CTTTGGGGAA CGTGTTTCAG GCACCCGGGG CTCTTCACCT GGATCAGGTG 
GCAGCAGCTG AGACCGGGGG GGTGCTGGGG GCCTTGACGC CTCTCCGAGC CGCAGACAAC 
GACTGCCGCA TAAACAAGCG CGTGGTCTTC TGGGCCGGGA ACTGCTCTGA GGCCGTGTGC 
GGGGCGGCCG ACTGCGCGCA GTGCACCAGG GAGGGCAAGT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CGCTGCTGAA TGTGTCCCCG ATGCCGGTGG AGTCATCACC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TACCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGGCCC AGGATGGGGG CGGCCGCTGC 
CTGGAGGGTG GACTCAGTGG CCCCCGTGAC GGGCTGACGT GTGGGCGACC TGGGGCCTCT 
TGGGCCTTTC TTTCCTGCCC CCCGGAGGAC GAATGTGCCA ACGGGCACCA TGACTGCAAT 
GAGACCCAGA ACTGCCACGA CCGGCCCCAC GGTTACGAGT GCAGCTGCAA GGCAGGCTAC 
ACCATGGACA ACGTGACGGG GCTGTGCCGC CCGGTGTGCG CCCAGGGTTG CGTGAATGGC 
TCGTGTGTGG AGCCCGACCA CTGCCGCTGC CACTTCGGCT TCGTTGGCCG CAACTGCTCC 
ACGGAGTGTC GCTGCAACCG TCACAGTGAC TGCGCCGGCG TGGGGGCCCG CGACCACTGC 
CTGCTCTGCC ACAACCACAC CAAGGGCAGC CACTGTGAGC AGTGCCTCCC ACTGTTCGTG 
GGTTCAGCCG TGGGGGGAGG GACCTGCCGG CCCTGCCACG CCTTTTGTCG CGGCAACAGC 
CACGTCTGCG TCTCCAGGAA GGAGCTTGAG ATAGCCAGGA GGGAGCCAGA GAAGTACTCG 
CTGGATCCAG ATGAGATTGA ACAGTGGGTG ACAGAGGGTC CCAGTGAAGA TGAGGCTGTG 
TGTGTGCACT GCCAGAATAA CAGCTACGGG GATAAGTGTG AGAGCTGCCT CCACGGCTAC 
TTTCTCCTCG ACAGGAAATG CATCAGATGC CAGTGTAATG GCCACGCGGA CACGTGTAAT 
GAGCAGGATG GGACAGGCTG CCCATGTCAG AACAACACAG AGACAGGCAC GTGCCAGGGC 
AGCTCCCCCA GTGACCGCCG AGACTGCTAC AAATACCAGT GTGCCAAGTG CCGAGAGTCA 
TTCCACGGGA GCCCGCTAGG GGGCCAGCAG TGCTACCGCC TCATCTCTGT GGAGCAGGAG 
TACTGCCTGG ACCCCACGTC CCAGACCAAC TGTTTCCACG AGCCCAAGCG CCGGGCTCTG 
GGCCCCGGCC GCACGGTTCT TTTTGGAGTG CAGCCCAAGT TCACCAATGT GGACATCCGC 
CTGACGCTGG ACGTGACCTT TGGCGCCGTG GACCTCTATG TCTCCACCTC CTATGACACC 
TTCGTGGTCC GCGTGGCTCC TGACACTGGT GTCCACACCG TGCATATCCA GCCGCCACCG 
CCCCCCCCAC CCCCCCCACC CCCCCCCTGG GGACCTGGGG ATCGGGGGGG ACCTGGGGCT 
GGAGGTGGGC CGGCTACCCC AGCAGAGCCG CGAGTACGGG AGGTCTGGCC ACGGGGCCTG 
ATCACCTACG TGACAGTGAC AGAGCCGTCG GCGGTGCTGG TGGTCCGTGG CGTGAGGGAC 
CGGCTGGTCA TCACCTACCC GCACGAGCAC CACACCCTCA AGTCCAGCCG CTTCTACCTG 
CTCCTGTTGG GTGTTGGAGA CCCAAGTGGG CCCAGTGCCA ACGGCTCAGC TGACTCGCAG 
GGCTTGCTCT TCTTCCGGCA GGACCAGGCC CACATTGACC TGTTTGTCTT CTTCTCCGTC 
TTTTTCTCCT GCTTCTTCCT CTTCCTCTCG CTCTGTGTGC TGCTCTGGAA GGCCAAGCAG 
GCCCTGGACC ACCGGCAGGA ACAGCGCCGG CACCTGCAGG AGATGACCAA GATGGCCAGC 
CGCCCCTTCG CCAAGGTCAC CGTCTGCTTC CCGCCGGACC CTGCTGCCCC AGCCCCTGCC 
TGGAAGCCTG CCGGGCTCCC GCCACCCACC TTCCGCCGCT CTGAGCCCTT CTTGGCACCC 
TTGCTGCTGA CGGGGGCCGG CGGGCCCTGG GGACCTGTAG GAGGCGGCTG CTGCCCACCG 
GCCATCCCCA CCACCACTGC CGGCCTGCGA GCCGGGCCTA TCACCCTTGA GCCCACCGAG 
GATGGCATGG CCGGCGTGGC CACACTGCTG CTCCAACTGC CTGGTGGGCC CCACGCACCC 
AACGGCGCCT GCCTGGGGTC AGCGCTCGTC ACACTGCGGC ACCGGCTGCA CGAGTACTGC 
GGCGGCGGCG GGGGCGCTGG GGGCAGTGGG CACGGGGCTG GTGCGGGCCG GAAGGGGCTG 
CTGAGCCAGG ACAACCTCAC CAGCATGTCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF8 ( XM_027978350.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027834151.1 Ovis aries multiple EGF like domains 8 (MEGF8), mRNA.

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CloneID	OSm64515
Clone ID Related Accession (Same CDS sequence)	XM_027978350.1
Accession Version	XM_027978350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	multiple epidermal growth factor-like domains protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040265.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTATAT CATTGGCCGT GCTGGGGCCA 
CTATCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTACG ACTACCTGTT TGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACGCG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTA 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCCG GCGGCTGCCG CAGCCACGGA CAGTGCCGGC CCCCGGGGGT GTGTGTCTGC 
GAGCCGGGCT GGGGGGGCCC CGACTGCGGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CACGGCACCT GCGCCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGT 
GCCTGTGACC TGCACTTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACCGGGG 
CTGCTGGCTG TTTTCGGAGG ACAGGACCTC AACCGGGCGC TGGGGGACCT CGTTCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGTACCCTCA CCAGCGACAT CTGGGCTTTT AGCCCAATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA CGCAGCAGCC 
CTTGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCAACAGC ACCAGTGGGG GCTACTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCTGCCACC GGACATTCCA TGGTGTTCCA CGCCCCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA CGTGGATCGG CGTGTATGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCAGG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCCGAGCT TGCCCCTCCA 
GGAACTCCGG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GCGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCCCG TGGGGACTTG 
ATGGCCTACA AAGTACCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGACTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGCTGGTGT 
CAGGGGGCCT GCCAAGCCGC CCCGCCTCCT GGGACTCCCT CGGGGGCTTG TCTGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCGGGG ACCCGGCGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGAGCAGGC CCGCTGCCGT GGGGAGCAGA TCTCTGGCAC CGTGGGCTGG 
TGGGGGCCCG CTCCTGTCTT TGTGACCTCC CTGGAGGCCT GTGTCACCCA GAGCTTCCTG 
CCTGGCCTGC ACCTGCTCAC CTTCCAGCAG CCACCCAACG CCTCCCAGCC TGACAAGGTC 
TTGATCGTCC GCAGCACGAC CATCACCCTG ACGCCGAGCT CAGAGACCGA TGTGTCCCTG 
GTCTACCGTG GCTTCATCCA CCCGCTGCTG CCGGGAGGCC CTGGGGGGTC TGGGGCTGAG 
GATGTGGCCG TGTGGGCCCG GGCGCAGCGC CTGCATGTGC TGGCCCGGAT GGCCCGGGGC 
CCAGATGCAG AAAACATGGA GGAGGTGGGG CGCTGGGTGG CACAGCAGGA GAAGGAGACG 
AGGCGGCTGC AACGCCCAGG GTCAGCTCGC CTCTTCCCAC TGCCCGGCCG GGGCCACAAG 
TATGCAGTAG AGATCCGGGG CCAGCTCAAT GGCTCAGCAG GCCCCGGGCA CAGCGAGCTC 
ACCCTGCTGT GGGACCGGAC TGGCGTGCCA GGTGGCAGCG AGATCTCCTT CTTCTTCCTG 
GAGCCCTACC GCTCGTCAGC CTGTGCCTCC TACTCCTCCT GCCTGGGTTG CCTGGCAGAC 
CAGGGCTGCG GCTGGTGCCT GGCCAGTGCC ACCTGCCACC TGCGCCAGAG CGGCGCCCAC 
TGCGGGGACG CTGGGGCCGG CGGGTCCCTG CTCGTGCTGG TGCCTGCCCT CTGCCCGCTC 
TGCGAGGAGC ATCGCGACTG CCACGCCTGC ACCCAGGACC CCTTCTGTGA GTGGCATCAG 
AGCACCAGCC GCAAGGGGGA CGCGGCGTGC AGCCGGCGCG GCCGGGGTCG GGGCGCCCTG 
AAGAGTCCGG AAGAGTGCCC CCCACTCTGC AGCCAGCGGC TGACCTGTGA CGACTGCCTG 
GCCAACTCGA GCCAGTGCGC CTGGTGCCAG TCCACCCGCA CCTGCTTCCT GTTCGCCGCC 
TACCTGGCCC GGTACCCGCA CGGGGGCTGC CGAGGCTGGG ACGACAGCGT GCACTCGGAG 
CCTCGGTGCC GGAGCTGTGA CCGATTCCAG AGCTGTCACG AGTGTCTGCA GAGCCACGAG 
TGTGGCTGGT GTGGAAACGA GGACAACCCC ACGCTGGGCC GTTGCCTCCA GGGGGACTTC 
TCAGGGCCTC TGAGTGAGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCATATGCC CGCTGTCCAG ATGTGGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTATGAATGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGTGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCGG ACCTGCCCCC TCCGACTCCT GCCCCCGGCC CCCCTGCGCC CCGCTGTTCA 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAC 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCCGG CAGCTTCGGG 
AACGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ATGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GCCTCCTGCC AGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTACAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAC 
GTCCTGGCTT TTGATGGATT CCCACGCTTC CTGGACACTG GTGTTGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTCTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTTCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAACGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCTGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGGT GGACTCTGCC GGTGTCCCCA AGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTT TGTGCTGAGG GCTTCGGGGG TCCTGACTGT 
GCCACAAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCTGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACGCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGCCT CACAGCTGGG GGCGTCTCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCTGTC 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGTGGGAC GCCCCCCACA GGTCTCTATG GTCACTCTGC TGTGTATCAC 
GAGGCCACCG ACTCCCTCTA CGTGTTTGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCATCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCATGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGTGACGT GCTGCTTTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCT GACCTCATCG GCCCAGCCTC CGTGGGCCCG 
CCAATGGAAG AGTCTGTGGC TCACGCTGTG GCCGCGGTAG GGGGTCGCCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCT TGTCCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC TGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAG 
GGGGCCTGCC TGTCCGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCGC CCCCTGCTCC 
CCTATGCCCC ACTCCCCTGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCG CTGCCCCCCG CTGTAAGTGG 
TGCACCAACT GCCCTGAGGG CGCCTGCATC GGGCGCAATG GGTCCTGCAC CTCAGAGAAC 
GACTGCCGCA TCAACCAGCG TGAGGTCTTC TGGGCCGGGA ACTGCTCTGA GGCCGTGTGC 
GGGGCGGCCG ACTGCGCGCA GTGCACCAGG GAGGGCAAGT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CGCTGCTGAA TGTGTCCCCG ATGCCGGTGG AGTCATCACC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TACCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGCGCCCTCT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGC CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CTTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ATGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTATGCC GCCCGGTGTG CGCCCAGGGT TGCGTGAATG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTTGGC CGCAACTGCT CCACGGAGTG TCGCTGCAAC 
CGTCACAGTG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAACCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCACTGTTCG TGGGTTCAGC CGTGGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGCAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCA GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACAGTGGG TGACAGAGGG TCCCAGTGAA GATGAGGCTG TGTGTGTGCA CTGCCAGAAT 
AACAGCTACG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTTCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACGTGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCT GTGGAGCAGG AGTACTGCCT GGACCCCACG 
TCCCAGACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGCCCCGG CCGCACGGTT 
CTTTTTGGAG TGCAGCCCAA GTTCACCAAT GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA TGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCTGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CGCCTCCCCC ACCCCCCCCA 
CCCCCTGCTG ACGGTGGTCC CCGGGGACCT GGGGATCTGG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTA CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
TTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTCTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC CGTCTTTTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACCGTCTG CTTCCCGCCG GACCCTGCTG CCCCAGCCCC TGCCTGGAAG 
CCTGCCGGGC TCCCGCCACC CACCTTCCGC CGCTCTGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACGGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGCG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGTG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAGTA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCACGGG GCTGGTGCGG GCCGGAAGGG GCTGCTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027834151.1
CDS	735..9104
Translation	MALGRALAVALAISLAVLGPLSPGAQAGDCKGQRQVLREAPGFVTDGAGNYSVNGNCEWLIEAPSSQHRILLDFLFLDTECTYDYLFVYDGDSPRGPLLASLSGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCRSHGQCRPPGVCVCEPGWGGPDCGLQECSAYCGSHGTCASPLGPCRCEPGFLGRACDLHLWENQGAGWWHNVSAGDPAFSARIGAAGAFLSPPGLLAVFGGQDLNRALGDLVLYNFSANTWERWDLSPAPAARHSHVAVAWAGSLVLMGGELADGTLTSDIWAFSPMGGGRWELLAPPASSPSGPPGLAGHAAALVDNTWLYVSGGRTQHDLFSSGLFRFRLNSTSGGYWEQVMPAGGRPPAATGHSMVFHAPSRALLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGREGLQGPRERAFHTASVLGNYMLVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAAPPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACLAASCLGLSRLLGDCQACLAFSSSAAPPRGPGALGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPGLHLLTFQQPPNASQPDKVLIVRSTTITLTPSSETDVSLVYRGFIHPLLPGGPGGSGAEDVAVWARAQRLHVLARMARGPDAENMEEVGRWVAQQEKETRRLQRPGSARLFPLPGRGHKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSACASYSSCLGCLADQGCGWCLASATCHLRQSGAHCGDAGAGGSLLVLVPALCPLCEEHRDCHACTQDPFCEWHQSTSRKGDAACSRRGRGRGALKSPEECPPLCSQRLTCDDCLANSSQCAWCQSTRTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCDRFQSCHECLQSHECGWCGNEDNPTLGRCLQGDFSGPLSEGNCSLWVGEGLGLSVALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTYCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLPPPTPAPGPPAPRCSRDCGCNFHSHCHKRGPGFCDECQDWTWGEHCERCRPGSFGNATGSGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRFGGLLPAGGGAARAGPGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGVPGGGLCRCPQGWAGPHCRMAVCPENCNAHNGAGTCNQSLGVCVCAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGVSRDFWVLNLTTLQWRQEKAPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPDLIGPASVGPPMEESVAHAVAAVGGRLFISGGFGGVALGRLLALSLPPDPCRLLPSPEACNQTGACTWCQGACLSGDQAHRLGCGGAPCSPMPHSPEECRRLRTCSECLARHPRTLQPGDGEAAAPRCKWCTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAVCGAADCAQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALCLRRPHCGWCTWGAQDGGGRCLEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKAGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSDCAGVGARDHCLLCHNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEIARREPEKYSLDPDEIEQWVTEGPSEDEAVCVHCQNNSYGDKCESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQEYCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPPPPADGGPRGPGDLGGPGAGGGPATPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRLVITYPHEHHTLKSSRFYLLLLGVGDPSGPSANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDHRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPAWKPAGLPPPTFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL

Target ORF information:

RefSeq Version	XM_027978350.1
Organism	Ovis aries(sheep)
Definition	Ovis aries multiple EGF like domains 8 (MEGF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027978350.1

ORF Insert Sequence:

ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTATAT CATTGGCCGT GCTGGGGCCA 
CTATCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT 
CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC 
ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA 
TGCACTTACG ACTACCTGTT TGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC 
AGTCTGAGTG GGAGCACGCG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTA 
CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC 
CTGTGCCCCG GCGGCTGCCG CAGCCACGGA CAGTGCCGGC CCCCGGGGGT GTGTGTCTGC 
GAGCCGGGCT GGGGGGGCCC CGACTGCGGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC 
CACGGCACCT GCGCCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGT 
GCCTGTGACC TGCACTTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC 
GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACCGGGG 
CTGCTGGCTG TTTTCGGAGG ACAGGACCTC AACCGGGCGC TGGGGGACCT CGTTCTGTAC 
AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC 
TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT 
GGTACCCTCA CCAGCGACAT CTGGGCTTTT AGCCCAATGG GTGGCGGCCG CTGGGAGCTT 
CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA CGCAGCAGCC 
CTTGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC 
TCCGGCCTCT TCCGTTTCCG CCTCAACAGC ACCAGTGGGG GCTACTGGGA GCAGGTGATG 
CCAGCAGGTG GGCGGCCCCC TGCTGCCACC GGACATTCCA TGGTGTTCCA CGCCCCCTCC 
CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG 
AACTCCACCG AGCTCTTCCA CGTGGATCGG CGTGTATGGA CCACCCTGAA GGGCCGGGAG 
GGACTTCAGG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG 
CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC 
ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCCGAGCT TGCCCCTCCA 
GGAACTCCGG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG 
CTTGGTGGCA GCGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCCCG TGGGGACTTG 
ATGGCCTACA AAGTACCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGACTA CCACTTGGAC 
TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGCTGGTGT 
CAGGGGGCCT GCCAAGCCGC CCCGCCTCCT GGGACTCCCT CGGGGGCTTG TCTGGCTGCC 
AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC 
AGTGCGGCTC CCCCCCGGGG ACCCGGCGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC 
CTCCCTCGGC CTGAGCAGGC CCGCTGCCGT GGGGAGCAGA TCTCTGGCAC CGTGGGCTGG 
TGGGGGCCCG CTCCTGTCTT TGTGACCTCC CTGGAGGCCT GTGTCACCCA GAGCTTCCTG 
CCTGGCCTGC ACCTGCTCAC CTTCCAGCAG CCACCCAACG CCTCCCAGCC TGACAAGGTC 
TTGATCGTCC GCAGCACGAC CATCACCCTG ACGCCGAGCT CAGAGACCGA TGTGTCCCTG 
GTCTACCGTG GCTTCATCCA CCCGCTGCTG CCGGGAGGCC CTGGGGGGTC TGGGGCTGAG 
GATGTGGCCG TGTGGGCCCG GGCGCAGCGC CTGCATGTGC TGGCCCGGAT GGCCCGGGGC 
CCAGATGCAG AAAACATGGA GGAGGTGGGG CGCTGGGTGG CACAGCAGGA GAAGGAGACG 
AGGCGGCTGC AACGCCCAGG GTCAGCTCGC CTCTTCCCAC TGCCCGGCCG GGGCCACAAG 
TATGCAGTAG AGATCCGGGG CCAGCTCAAT GGCTCAGCAG GCCCCGGGCA CAGCGAGCTC 
ACCCTGCTGT GGGACCGGAC TGGCGTGCCA GGTGGCAGCG AGATCTCCTT CTTCTTCCTG 
GAGCCCTACC GCTCGTCAGC CTGTGCCTCC TACTCCTCCT GCCTGGGTTG CCTGGCAGAC 
CAGGGCTGCG GCTGGTGCCT GGCCAGTGCC ACCTGCCACC TGCGCCAGAG CGGCGCCCAC 
TGCGGGGACG CTGGGGCCGG CGGGTCCCTG CTCGTGCTGG TGCCTGCCCT CTGCCCGCTC 
TGCGAGGAGC ATCGCGACTG CCACGCCTGC ACCCAGGACC CCTTCTGTGA GTGGCATCAG 
AGCACCAGCC GCAAGGGGGA CGCGGCGTGC AGCCGGCGCG GCCGGGGTCG GGGCGCCCTG 
AAGAGTCCGG AAGAGTGCCC CCCACTCTGC AGCCAGCGGC TGACCTGTGA CGACTGCCTG 
GCCAACTCGA GCCAGTGCGC CTGGTGCCAG TCCACCCGCA CCTGCTTCCT GTTCGCCGCC 
TACCTGGCCC GGTACCCGCA CGGGGGCTGC CGAGGCTGGG ACGACAGCGT GCACTCGGAG 
CCTCGGTGCC GGAGCTGTGA CCGATTCCAG AGCTGTCACG AGTGTCTGCA GAGCCACGAG 
TGTGGCTGGT GTGGAAACGA GGACAACCCC ACGCTGGGCC GTTGCCTCCA GGGGGACTTC 
TCAGGGCCTC TGAGTGAGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT 
GTGGCCCTCC CCGCCCGCTG GGCATATGCC CGCTGTCCAG ATGTGGACGA GTGCCGCCTG 
GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTATGAATGT 
CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG 
GACTGTGGCC ACGGTGTGTG CAGTGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC 
TGGACATCGG ACCTGCCCCC TCCGACTCCT GCCCCCGGCC CCCCTGCGCC CCGCTGTTCA 
CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAC 
GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCCGG CAGCTTCGGG 
AACGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ATGGGCATGG GGACCCTCGC 
CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC 
CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC 
CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC 
CGGTTTGGGG GCCTCCTGCC AGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC 
TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTACAGC CCTGTGCCCC CGGGACCCTC 
TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAC 
GTCCTGGCTT TTGATGGATT CCCACGCTTC CTGGACACTG GTGTTGTCCA GTCGGACCGT 
AGCCTCATCG CTGCGTTCTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG 
TCTGGGCTGC TGGTACTTCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAACGCC 
TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCTGGGA GCTGCCCTGT CCCCCAGGAG 
TGTGTGCCCC AGGATGGGGT CCCAGGTGGT GGACTCTGCC GGTGTCCCCA AGGCTGGGCT 
GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG 
ACGTGTAATC AGAGCCTGGG CGTGTGCGTT TGTGCTGAGG GCTTCGGGGG TCCTGACTGT 
GCCACAAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA 
GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT 
GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT 
AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCTGA GGATGGGGGC 
CCAGGCCCCT CCCCTCGCTC TTTCCACGCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC 
ATGTACCTGC TGGGGGGCCT CACAGCTGGG GGCGTCTCCC GGGACTTCTG GGTCCTCAAC 
CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCTGTC 
GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC 
CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG 
TCCGGGGCTC AGAGTGGGAC GCCCCCCACA GGTCTCTATG GTCACTCTGC TGTGTATCAC 
GAGGCCACCG ACTCCCTCTA CGTGTTTGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC 
CCATCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT 
TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCATGCCT CGGCCTTGCT AGGGGACACC 
ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGTGACGT GCTGCTTTAC 
CAGGTCAACT GCAACACCTG GCTCCTGCCT GACCTCATCG GCCCAGCCTC CGTGGGCCCG 
CCAATGGAAG AGTCTGTGGC TCACGCTGTG GCCGCGGTAG GGGGTCGCCT GTTTATCTCA 
GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCT TGTCCCTGCC CCCTGACCCC 
TGCCGCCTGC TGCCCTCGCC TGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAG 
GGGGCCTGCC TGTCCGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCGC CCCCTGCTCC 
CCTATGCCCC ACTCCCCTGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC 
CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCG CTGCCCCCCG CTGTAAGTGG 
TGCACCAACT GCCCTGAGGG CGCCTGCATC GGGCGCAATG GGTCCTGCAC CTCAGAGAAC 
GACTGCCGCA TCAACCAGCG TGAGGTCTTC TGGGCCGGGA ACTGCTCTGA GGCCGTGTGC 
GGGGCGGCCG ACTGCGCGCA GTGCACCAGG GAGGGCAAGT GCATGTGGAC GCGGCAGTTC 
AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC 
TTCAGCCACT CGCTGCTGAA TGTGTCCCCG ATGCCGGTGG AGTCATCACC CCCGCTGCCC 
TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT 
GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC 
TACCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC 
TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGCGCCCTCT GCCTGCGGCG CCCCCACTGT 
GGCTGGTGTA CCTGGGGGGC CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT 
GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CTTGGGCCTT TCTTTCCTGC 
CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ATGAGACCCA GAACTGCCAC 
GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG 
GGGCTATGCC GCCCGGTGTG CGCCCAGGGT TGCGTGAATG GCTCGTGTGT GGAGCCCGAC 
CACTGCCGCT GCCACTTCGG CTTCGTTGGC CGCAACTGCT CCACGGAGTG TCGCTGCAAC 
CGTCACAGTG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAACCAC 
ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCACTGTTCG TGGGTTCAGC CGTGGGGGGA 
GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGCAACA GCCACGTCTG CGTCTCCAGG 
AAGGAGCTTG AGATAGCCAG GAGGGAGCCA GAGAAGTACT CGCTGGATCC AGATGAGATT 
GAACAGTGGG TGACAGAGGG TCCCAGTGAA GATGAGGCTG TGTGTGTGCA CTGCCAGAAT 
AACAGCTACG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTTCTCCT CGACGGGAAA 
TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACGTGTA ATGAGCAGGA TGGGACAGGC 
TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC 
CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA 
GGGGGCCAGC AGTGCTACCG CCTCATCTCT GTGGAGCAGG AGTACTGCCT GGACCCCACG 
TCCCAGACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGCCCCGG CCGCACGGTT 
CTTTTTGGAG TGCAGCCCAA GTTCACCAAT GTGGACATCC GCCTGACGCT GGACGTGACC 
TTTGGCGCCG TGGACCTCTA TGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT 
CCTGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CGCCTCCCCC ACCCCCCCCA 
CCCCCTGCTG ACGGTGGTCC CCGGGGACCT GGGGATCTGG GGGGACCTGG GGCTGGAGGT 
GGGCCGGCTA CCCCAGCAGA GCCGCGAGTA CGGGAGGTCT GGCCACGGGG CCTGATCACC 
TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG 
GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG 
TTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG 
CTCTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC CGTCTTTTTC 
TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG 
GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC 
TTCGCCAAGG TCACCGTCTG CTTCCCGCCG GACCCTGCTG CCCCAGCCCC TGCCTGGAAG 
CCTGCCGGGC TCCCGCCACC CACCTTCCGC CGCTCTGAGC CCTTCTTGGC ACCCTTGCTG 
CTGACGGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGCG GCTGCTGCCC ACCGGCCATC 
CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC 
ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGTG GGCCCCACGC ACCCAACGGC 
GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAGTA CTGCGGCGGC 
GGCGGGGGCG CTGGGGGCAG TGGGCACGGG GCTGGTGCGG GCCGGAAGGG GCTGCTGAGC 
CAGGACAACC TCACCAGCAT GTCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MEGF8 cDNA ORF clone, Ovis aries(sheep)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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