Rhox13 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhox13
Entrez Gene ID	73614
Full Name	reproductive homeobox 13
Synonyms	1700123J19Rik
General protein information	Preferred Names reproductive homeobox 13 Names homeobox protein Rhox13 novel homeobox domain-containing protein reproductive homeobox protein 13
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X A3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001185002.1	NP_001171931.1	homeobox protein Rhox13

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rhox13	NP_001171931.1
Rattus norvegicus (Norway rat)	Rhox13	XP_001077350.3

Gene Ontology
Bibliography

Related articles in PubMed

Rhox13 is required for a quantitatively normal first wave of spermatogenesis in mice.
Busada JT, Velte EK, Serra N, Cook K, Niedenberger BA, Willis WD, Goulding EH, Eddy EM, Geyer CB
Reproduction (Cambridge, England)152(5)379-88(2016 11)

The Homeobox Transcription Factor RHOX10 Drives Mouse Spermatogonial Stem Cell Establishment.
Song HW, Bettegowda A, Lake BB, Zhao AH, Skarbrevik D, Babajanian E, Sukhwani M, Shum EY, Phan MH, Plank TM, Richardson ME, Ramaiah M, Sridhar V, de Rooij DG, Orwig KE, Zhang K, Wilkinson MF
Cell reports17(1)149-164(2016 09)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

shRNA off-target effects in vivo: impaired endogenous siRNA expression and spermatogenic defects.
Song HW, Bettegowda A, Oliver D, Yan W, Phan MH, de Rooij DG, Corbett MA, Wilkinson MF
PloS one10(3)e0118549(2015)

Rhox13 is translated in premeiotic germ cells in male and female mice and is regulated by NANOS2 in the male.
Geyer CB, Saba R, Kato Y, Anderson AJ, Chappell VK, Saga Y, Eddy EM
Biology of reproduction86(4)127(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rhox13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhox13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12842	NM_001185002.1 Latest version!	Mus musculus reproductive homeobox 13 (Rhox13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhox13 ( NM_001185002.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171931.1 Mus musculus reproductive homeobox 13 (Rhox13), mRNA.

Price & Availability↑

CloneID	OMu12842
Clone ID Related Accession (Same CDS sequence)	NM_001185002.1
Accession Version	NM_001185002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550678400000
Organism	Mus musculus(house mouse)
Product	homeobox protein Rhox13
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL512346.41, AV490373.1 and AK007253.1. On or before May 29, 2010 this sequence version replaced XM_356401.5, XM_976021.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK007253.1, CF356891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCACG GGGTCCCTTT TGACCACAAT TACTACATTG TGGAGTGCAA GGAGGAAACA 
AATGCTGAAG CCCAGGCAGG GGCCATGGCC GCCACCTCAA CCGAGGAGGC CCCAGGGGCT 
GTGGAGGTGG CGCAGGCAGC AGTGGCGAGC AGCCACGACA GTGGAGGAGC CATAGGCTGT 
GCCACTGTCA AGGAGAGTGA GAGCGACTCG GAGAGTGAGA GCGACTCGGA GAGTGAGAGC 
GACTCCTCCG ACTCCTCCGA CGAGAGTGAT GACGACTCGA GCACCAGCGA CGAGGATACT 
TCAGATCCTG AGGAGGCGGC AGCTCCCTCG GTGGCTGCGG TGGCTGCTGC TGCCGCTCCT 
CCAACTGTTC CTGCTGCCGC TGCTATTCAG ATTCCGGGGC CGTACCGCTA CCGCCCCCCT 
CGCCGCCATG TTCGCCGTCG TCGCCGTGGA CCGCCATTCC ACTTCGCACA GTGGCAGGTC 
GAGGAAATGG AAAGCCTGTT TGAAGAGACC CAGTACCCGG ATTTGCTTAC AAGGGGAGAG 
CTTGCAAGAA CTTTGAATGT GCCTGAGGTC AAAGTGAAGG TGTGGTTTAC CAATCGGAGA 
GCGAAGCAGA GGAAGATTGA GAGACGTGAA ATGCTGAGAA ATATTCCGCC TGGTGCTGAA 
GACTTCATTT TCATAACCGA TTTTGAAGAG CCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171931.1
CDS	451..1149
Translation	MAHGVPFDHNYYIVECKEETNAEAQAGAMAATSTEEAPGAVEVAQAAVASSHDSGGAIGCATVKESESDSESESDSESESDSSDSSDESDDDSSTSDEDTSDPEEAAAPSVAAVAAAAAPPTVPAAAAIQIPGPYRYRPPRRHVRRRRRGPPFHFAQWQVEEMESLFEETQYPDLLTRGELARTLNVPEVKVKVWFTNRRAKQRKIERREMLRNIPPGAEDFIFITDFEEPS

Target ORF information:

RefSeq Version	NM_001185002.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus reproductive homeobox 13 (Rhox13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185002.1

ORF Insert Sequence:

ATGGCCCACG GGGTCCCTTT TGACCACAAT TACTACATTG TGGAGTGCAA GGAGGAAACA 
AATGCTGAAG CCCAGGCAGG GGCCATGGCC GCCACCTCAA CCGAGGAGGC CCCAGGGGCT 
GTGGAGGTGG CGCAGGCAGC AGTGGCGAGC AGCCACGACA GTGGAGGAGC CATAGGCTGT 
GCCACTGTCA AGGAGAGTGA GAGCGACTCG GAGAGTGAGA GCGACTCGGA GAGTGAGAGC 
GACTCCTCCG ACTCCTCCGA CGAGAGTGAT GACGACTCGA GCACCAGCGA CGAGGATACT 
TCAGATCCTG AGGAGGCGGC AGCTCCCTCG GTGGCTGCGG TGGCTGCTGC TGCCGCTCCT 
CCAACTGTTC CTGCTGCCGC TGCTATTCAG ATTCCGGGGC CGTACCGCTA CCGCCCCCCT 
CGCCGCCATG TTCGCCGTCG TCGCCGTGGA CCGCCATTCC ACTTCGCACA GTGGCAGGTC 
GAGGAAATGG AAAGCCTGTT TGAAGAGACC CAGTACCCGG ATTTGCTTAC AAGGGGAGAG 
CTTGCAAGAA CTTTGAATGT GCCTGAGGTC AAAGTGAAGG TGTGGTTTAC CAATCGGAGA 
GCGAAGCAGA GGAAGATTGA GAGACGTGAA ATGCTGAGAA ATATTCCGCC TGGTGCTGAA 
GACTTCATTT TCATAACCGA TTTTGAAGAG CCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rhox13 is required for a quantitatively normal first wave of spermatogenesis in mice.
Reproduction (Cambridge, England)152(5)379-88(2016 11)
Busada JT,Velte EK,Serra N,Cook K,Niedenberger BA,Willis WD,Goulding EH,Eddy EM,Geyer CB

The Homeobox Transcription Factor RHOX10 Drives Mouse Spermatogonial Stem Cell Establishment.
Cell reports17(1)149-164(2016 09)
Song HW,Bettegowda A,Lake BB,Zhao AH,Skarbrevik D,Babajanian E,Sukhwani M,Shum EY,Phan MH,Plank TM,Richardson ME,Ramaiah M,Sridhar V,de Rooij DG,Orwig KE,Zhang K,Wilkinson MF

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

shRNA off-target effects in vivo: impaired endogenous siRNA expression and spermatogenic defects.
PloS one10(3)e0118549(2015)
Song HW,Bettegowda A,Oliver D,Yan W,Phan MH,de Rooij DG,Corbett MA,Wilkinson MF

Rhox13 is translated in premeiotic germ cells in male and female mice and is regulated by NANOS2 in the male.
Biology of reproduction86(4)127(2012 Apr)
Geyer CB,Saba R,Kato Y,Anderson AJ,Chappell VK,Saga Y,Eddy EM

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Rhox13 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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