Nsun4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nsun4
Entrez Gene ID	72181
Full Name	NOL1/NOP2/Sun domain family, member 4
Synonyms	2310010O12Rik,2810405F18Rik,AI507900,Shtap
General protein information	Preferred Names NOL1/NOP2/Sun domain family, member 4 Names putative methyltransferase NSUN4 sperm head and tail associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001369076.1	NP_001356005.1	putative methyltransferase NSUN4 isoform b
XM_006503407.3	XP_006503470.1	putative methyltransferase NSUN4 isoform X1
NM_028142.4	NP_082418.1	putative methyltransferase NSUN4
NM_028142.5	NP_082418.1	putative methyltransferase NSUN4 isoform a
NM_001369075.1	NP_001356004.1	putative methyltransferase NSUN4 isoform b

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nsun4	NP_001100148.1
Caenorhabditis elegans (roundworm)	CELE_Y39G10AR.21	NP_490958.1
Xenopus tropicalis (tropical clawed frog)	LOC101734691	XP_004920926.1
Mus musculus (house mouse)	Nsun4	NP_082418.1
Danio rerio (zebrafish)	nsun4	NP_001008649.1
Bos taurus (cattle)	NSUN4	NP_001178310.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008523	XP_554918.3
Xenopus tropicalis (tropical clawed frog)	nsun4	NP_001007930.1
Gallus gallus (chicken)	NSUN4	NP_001264521.1
Drosophila melanogaster (fruit fly)	CG4749	NP_608573.1
Pan troglodytes (chimpanzee)	NSUN4	XP_003308124.1
Homo sapiens (human)	NSUN4	NP_950245.2
Macaca mulatta (Rhesus monkey)	NSUN4	XP_001108301.1
Canis lupus familiaris (dog)	NSUN4	XP_005629091.1

Gene Ontology
Bibliography

Related articles in PubMed

NSUN4 is a dual function mitochondrial protein required for both methylation of 12S rRNA and coordination of mitoribosomal assembly.
Metodiev MD, Spåhr H, Loguercio Polosa P, Meharg C, Becker C, Altmueller J, Habermann B, Larsson NG, Ruzzenente B
PLoS genetics10(2)e1004110(2014 Feb)

Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase family genes in early mouse embryogenesis.
Chi L, Delgado-Olguín P
Gene expression patterns : GEP13(8)319-27(2013 Dec)

MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome.
Cámara Y, Asin-Cayuela J, Park CB, Metodiev MD, Shi Y, Ruzzenente B, Kukat C, Habermann B, Wibom R, Hultenby K, Franz T, Erdjument-Bromage H, Tempst P, Hallberg BM, Gustafsson CM, Larsson NG
Cell metabolism13(5)527-39(2011 May)

A novel protein, sperm head and tail associated protein (SHTAP), interacts with cysteine-rich secretory protein 2 (CRISP2) during spermatogenesis in the mouse.
Jamsai D, Rijal S, Bianco DM, O'Connor AE, Merriner DJ, Smith SJ, Gibbs GM, O'Bryan MK
Biology of the cell102(2)93-106(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nsun4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nsun4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84702	NM_001369075.1 Latest version!	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu84702	NM_001369076.1 Latest version!	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu33821	XM_006503407.3 Latest version!	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu16191	NM_028142.5 Latest version!	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OMu16191	NM_028142.4	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nsun4 ( NM_001369076.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356005.1 Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84702
Clone ID Related Accession (Same CDS sequence)	NM_001369076.1 , NM_001369075.1
Accession Version	NM_001369076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1552752000000
Organism	Mus musculus(house mouse)
Product	putative methyltransferase NSUN4 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627105.13. On Mar 18, 2019 this sequence version replaced XR_001784203.1. Transcript Variant: This variant (3), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FJ882982.1, SRR5189685.152153.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGACCTACA GTGTACAGTT TGGGGATCTT TGGCCATCAA TCCGTGTTAG TCTTCTCTCA 
GAGCAGAAGT ATGGTGCATT GGTCAATAAT TTTGCTGCTT GGGATAGTGT GAGTGCTAAG 
CTGGAGCAGC TGAGTGCCAA GGACTTCGTC AGTGAAGCCA TCTCTCACCA GAAACTGGAG 
CCTGAGAGTG GCCTCTCTCC AACTCCATCC CTGGACTGCA GCCCAAATCT TCGATGCTTC 
ACTTTTTCCA GAGGGGACGT CAGCCGCTTT CCTCCTGCCA GACTTGGCAG CCTGGGTCTC 
ATGGACTACT ACCTGATGGA TGCTGCCTCC TTGCTCCCTG TCCTGGCTCT TGGTCTGCAG 
CATGGAGACA CTGTGCTTGA TCTGTGTGCT GCTCCTGGGG GCAAAACGCT GGCATTACTT 
CAGACGGGTT GTTGCCGTAA TCTAGCTGCC AACGACCTCT CCACTTCCCG AACAGGCAGA 
CTGCAGAAGG TCCTTCACAG TTATGTGCCT CAAGATATTA GGGAAGGGAA CCAAGTCCGA 
GTTACCTCAT GGGATGGCAG GAAGTGGGGA GAACTGGAGG GGGACACCTA TGATAGGGTG 
TTAGTAGATG TGCCATGTAC CACGGACCGC CATTCCCTTC ATGAGGAAGA GAACAACATC 
TTTCAGCGGT CAAGGAAGAA GGAGCGACAG ATGTTGCCTA TGCTGCAGGT GCAGCTTCTT 
GCGGCTGGAC TCCTTGCCAC CAAACCAGGA GGTCACGTTG TCTATTCAAC TTGCTCACTG 
TCTCACTTAC AGAATGAGTA TGTGGTGCAA GGTGCCATTG AGCTTCTGGC CAATCAATAC 
AACATCAAGG TTCAAGTGGA AGACCTGTCT CACTTCCGAA AGCTTTTCAT GGACACATTC 
TGTTTCTTCC CATCCTGCCA GGTTGGGGAG CTGGTAATAC CAAACCTCAT GGTCAATTTT 
GGACCTATGT ACTTCTGCAA ACTGCATAGG CTGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356005.1
CDS	2888..3886
Translation	MTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDSVSAKLEQLSAKDFVSEAISHQKLEPESGLSPTPSLDCSPNLRCFTFSRGDVSRFPPARLGSLGLMDYYLMDAASLLPVLALGLQHGDTVLDLCAAPGGKTLALLQTGCCRNLAANDLSTSRTGRLQKVLHSYVPQDIREGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFQRSRKKERQMLPMLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYNIKVQVEDLSHFRKLFMDTFCFFPSCQVGELVIPNLMVNFGPMYFCKLHRLP

Target ORF information:

RefSeq Version	NM_001369076.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369076.1

ORF Insert Sequence:

ATGACCTACA GTGTACAGTT TGGGGATCTT TGGCCATCAA TCCGTGTTAG TCTTCTCTCA 
GAGCAGAAGT ATGGTGCATT GGTCAATAAT TTTGCTGCTT GGGATAGTGT GAGTGCTAAG 
CTGGAGCAGC TGAGTGCCAA GGACTTCGTC AGTGAAGCCA TCTCTCACCA GAAACTGGAG 
CCTGAGAGTG GCCTCTCTCC AACTCCATCC CTGGACTGCA GCCCAAATCT TCGATGCTTC 
ACTTTTTCCA GAGGGGACGT CAGCCGCTTT CCTCCTGCCA GACTTGGCAG CCTGGGTCTC 
ATGGACTACT ACCTGATGGA TGCTGCCTCC TTGCTCCCTG TCCTGGCTCT TGGTCTGCAG 
CATGGAGACA CTGTGCTTGA TCTGTGTGCT GCTCCTGGGG GCAAAACGCT GGCATTACTT 
CAGACGGGTT GTTGCCGTAA TCTAGCTGCC AACGACCTCT CCACTTCCCG AACAGGCAGA 
CTGCAGAAGG TCCTTCACAG TTATGTGCCT CAAGATATTA GGGAAGGGAA CCAAGTCCGA 
GTTACCTCAT GGGATGGCAG GAAGTGGGGA GAACTGGAGG GGGACACCTA TGATAGGGTG 
TTAGTAGATG TGCCATGTAC CACGGACCGC CATTCCCTTC ATGAGGAAGA GAACAACATC 
TTTCAGCGGT CAAGGAAGAA GGAGCGACAG ATGTTGCCTA TGCTGCAGGT GCAGCTTCTT 
GCGGCTGGAC TCCTTGCCAC CAAACCAGGA GGTCACGTTG TCTATTCAAC TTGCTCACTG 
TCTCACTTAC AGAATGAGTA TGTGGTGCAA GGTGCCATTG AGCTTCTGGC CAATCAATAC 
AACATCAAGG TTCAAGTGGA AGACCTGTCT CACTTCCGAA AGCTTTTCAT GGACACATTC 
TGTTTCTTCC CATCCTGCCA GGTTGGGGAG CTGGTAATAC CAAACCTCAT GGTCAATTTT 
GGACCTATGT ACTTCTGCAA ACTGCATAGG CTGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsun4 ( NM_001369075.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356004.1 Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84702
Clone ID Related Accession (Same CDS sequence)	NM_001369076.1 , NM_001369075.1
Accession Version	NM_001369075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1552752000000
Organism	Mus musculus(house mouse)
Product	putative methyltransferase NSUN4 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627105.13. Transcript Variant: This variant (2), as well as variant 3, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5189685.155230.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGACCTACA GTGTACAGTT TGGGGATCTT TGGCCATCAA TCCGTGTTAG TCTTCTCTCA 
GAGCAGAAGT ATGGTGCATT GGTCAATAAT TTTGCTGCTT GGGATAGTGT GAGTGCTAAG 
CTGGAGCAGC TGAGTGCCAA GGACTTCGTC AGTGAAGCCA TCTCTCACCA GAAACTGGAG 
CCTGAGAGTG GCCTCTCTCC AACTCCATCC CTGGACTGCA GCCCAAATCT TCGATGCTTC 
ACTTTTTCCA GAGGGGACGT CAGCCGCTTT CCTCCTGCCA GACTTGGCAG CCTGGGTCTC 
ATGGACTACT ACCTGATGGA TGCTGCCTCC TTGCTCCCTG TCCTGGCTCT TGGTCTGCAG 
CATGGAGACA CTGTGCTTGA TCTGTGTGCT GCTCCTGGGG GCAAAACGCT GGCATTACTT 
CAGACGGGTT GTTGCCGTAA TCTAGCTGCC AACGACCTCT CCACTTCCCG AACAGGCAGA 
CTGCAGAAGG TCCTTCACAG TTATGTGCCT CAAGATATTA GGGAAGGGAA CCAAGTCCGA 
GTTACCTCAT GGGATGGCAG GAAGTGGGGA GAACTGGAGG GGGACACCTA TGATAGGGTG 
TTAGTAGATG TGCCATGTAC CACGGACCGC CATTCCCTTC ATGAGGAAGA GAACAACATC 
TTTCAGCGGT CAAGGAAGAA GGAGCGACAG ATGTTGCCTA TGCTGCAGGT GCAGCTTCTT 
GCGGCTGGAC TCCTTGCCAC CAAACCAGGA GGTCACGTTG TCTATTCAAC TTGCTCACTG 
TCTCACTTAC AGAATGAGTA TGTGGTGCAA GGTGCCATTG AGCTTCTGGC CAATCAATAC 
AACATCAAGG TTCAAGTGGA AGACCTGTCT CACTTCCGAA AGCTTTTCAT GGACACATTC 
TGTTTCTTCC CATCCTGCCA GGTTGGGGAG CTGGTAATAC CAAACCTCAT GGTCAATTTT 
GGACCTATGT ACTTCTGCAA ACTGCATAGG CTGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356004.1
CDS	2969..3967
Translation	MTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDSVSAKLEQLSAKDFVSEAISHQKLEPESGLSPTPSLDCSPNLRCFTFSRGDVSRFPPARLGSLGLMDYYLMDAASLLPVLALGLQHGDTVLDLCAAPGGKTLALLQTGCCRNLAANDLSTSRTGRLQKVLHSYVPQDIREGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFQRSRKKERQMLPMLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYNIKVQVEDLSHFRKLFMDTFCFFPSCQVGELVIPNLMVNFGPMYFCKLHRLP

Target ORF information:

RefSeq Version	NM_001369075.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369075.1

ORF Insert Sequence:

ATGACCTACA GTGTACAGTT TGGGGATCTT TGGCCATCAA TCCGTGTTAG TCTTCTCTCA 
GAGCAGAAGT ATGGTGCATT GGTCAATAAT TTTGCTGCTT GGGATAGTGT GAGTGCTAAG 
CTGGAGCAGC TGAGTGCCAA GGACTTCGTC AGTGAAGCCA TCTCTCACCA GAAACTGGAG 
CCTGAGAGTG GCCTCTCTCC AACTCCATCC CTGGACTGCA GCCCAAATCT TCGATGCTTC 
ACTTTTTCCA GAGGGGACGT CAGCCGCTTT CCTCCTGCCA GACTTGGCAG CCTGGGTCTC 
ATGGACTACT ACCTGATGGA TGCTGCCTCC TTGCTCCCTG TCCTGGCTCT TGGTCTGCAG 
CATGGAGACA CTGTGCTTGA TCTGTGTGCT GCTCCTGGGG GCAAAACGCT GGCATTACTT 
CAGACGGGTT GTTGCCGTAA TCTAGCTGCC AACGACCTCT CCACTTCCCG AACAGGCAGA 
CTGCAGAAGG TCCTTCACAG TTATGTGCCT CAAGATATTA GGGAAGGGAA CCAAGTCCGA 
GTTACCTCAT GGGATGGCAG GAAGTGGGGA GAACTGGAGG GGGACACCTA TGATAGGGTG 
TTAGTAGATG TGCCATGTAC CACGGACCGC CATTCCCTTC ATGAGGAAGA GAACAACATC 
TTTCAGCGGT CAAGGAAGAA GGAGCGACAG ATGTTGCCTA TGCTGCAGGT GCAGCTTCTT 
GCGGCTGGAC TCCTTGCCAC CAAACCAGGA GGTCACGTTG TCTATTCAAC TTGCTCACTG 
TCTCACTTAC AGAATGAGTA TGTGGTGCAA GGTGCCATTG AGCTTCTGGC CAATCAATAC 
AACATCAAGG TTCAAGTGGA AGACCTGTCT CACTTCCGAA AGCTTTTCAT GGACACATTC 
TGTTTCTTCC CATCCTGCCA GGTTGGGGAG CTGGTAATAC CAAACCTCAT GGTCAATTTT 
GGACCTATGT ACTTCTGCAA ACTGCATAGG CTGCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsun4 ( XM_006503407.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503470.1 Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu33821
Clone ID Related Accession (Same CDS sequence)	XM_006503407.3
Accession Version	XM_006503407.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	putative methyltransferase NSUN4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503407.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGC CCGTCTTAAG ATGTGTTCGG AAGCTGCTGA AGCTCGTGGA CTTCACGCCG 
GTCCCGCGGA GATATCGATA TAAGAAGAAA TGGGCTACCA CAGAACCACA ATTCACGGCC 
AGTCGGCTTG CTCTGCAGAA TTTTGACATG ACCTACAGTG TACAGTTTGG GGATCTTTGG 
CCATCAATCC GTGTTAGTCT TCTCTCAGAG CAGAAGTATG GTGCATTGGT CAATAATTTT 
GCTGCTTGGG ATAGTGTGAG TGCTAAGCTG GAGCAGCTGA GTGCCAAGGA CTTCGTCAGT 
GAAGCCATCT CTCACCAGAA ACTGGAGCCT GAGAGTGGCC TCTCTCCAAC TCCATCCCTG 
GACTGCAGCC CAAATCTTCG ATGCTTCACT TTTTCCAGAG GGGACGTCAG CCGCTTTCCT 
CCTGCCAGAC TTGGCAGCCT GGGTCTCATG GACTACTACC TGATGGATGC TGCCTCCTTG 
CTCCCTGTCC TGGCTCTTGG TCTGCAGCAT GGAGACACTG TGCTTGATCT GTGTGCTGCT 
CCTGGGGGCA AAACGCTGGC ATTACTTCAG ACGGGTTGTT GCCGTACTCA CCCAGGCCTG 
GCTTCTTGGC CGCGGAACAT AGACTGGGTC ACAGGAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503470.1
CDS	94..735
Translation	MAAPVLRCVRKLLKLVDFTPVPRRYRYKKKWATTEPQFTASRLALQNFDMTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDSVSAKLEQLSAKDFVSEAISHQKLEPESGLSPTPSLDCSPNLRCFTFSRGDVSRFPPARLGSLGLMDYYLMDAASLLPVLALGLQHGDTVLDLCAAPGGKTLALLQTGCCRTHPGLASWPRNIDWVTGT

Target ORF information:

RefSeq Version	XM_006503407.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503407.3

ORF Insert Sequence:

ATGGCTGCGC CCGTCTTAAG ATGTGTTCGG AAGCTGCTGA AGCTCGTGGA CTTCACGCCG 
GTCCCGCGGA GATATCGATA TAAGAAGAAA TGGGCTACCA CAGAACCACA ATTCACGGCC 
AGTCGGCTTG CTCTGCAGAA TTTTGACATG ACCTACAGTG TACAGTTTGG GGATCTTTGG 
CCATCAATCC GTGTTAGTCT TCTCTCAGAG CAGAAGTATG GTGCATTGGT CAATAATTTT 
GCTGCTTGGG ATAGTGTGAG TGCTAAGCTG GAGCAGCTGA GTGCCAAGGA CTTCGTCAGT 
GAAGCCATCT CTCACCAGAA ACTGGAGCCT GAGAGTGGCC TCTCTCCAAC TCCATCCCTG 
GACTGCAGCC CAAATCTTCG ATGCTTCACT TTTTCCAGAG GGGACGTCAG CCGCTTTCCT 
CCTGCCAGAC TTGGCAGCCT GGGTCTCATG GACTACTACC TGATGGATGC TGCCTCCTTG 
CTCCCTGTCC TGGCTCTTGG TCTGCAGCAT GGAGACACTG TGCTTGATCT GTGTGCTGCT 
CCTGGGGGCA AAACGCTGGC ATTACTTCAG ACGGGTTGTT GCCGTACTCA CCCAGGCCTG 
GCTTCTTGGC CGCGGAACAT AGACTGGGTC ACAGGAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsun4 ( NM_028142.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082418.1 Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), mRNA.

Price & Availability↑

CloneID	OMu16191
Clone ID Related Accession (Same CDS sequence)	NM_028142.4 , NM_028142.5
Accession Version	NM_028142.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Mus musculus(house mouse)
Product	putative methyltransferase NSUN4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK012994.1, AL627105.13 and AW489697.1. On Dec 31, 2009 this sequence version replaced NM_028142.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK012994.1, BC024628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGC CCGTCTTAAG ATGTGTTCGG AAGCTGCTGA AGCTCGTGGA CTTCACGCCG 
GTCCCGCGGA GATATCGATA TAAGAAGAAA TGGGCTACCA CAGAACCACA ATTCACGGCC 
AGTCGGCTTG CTCTGCAGAA TTTTGACATG ACCTACAGTG TACAGTTTGG GGATCTTTGG 
CCATCAATCC GTGTTAGTCT TCTCTCAGAG CAGAAGTATG GTGCATTGGT CAATAATTTT 
GCTGCTTGGG ATAGTGTGAG TGCTAAGCTG GAGCAGCTGA GTGCCAAGGA CTTCGTCAGT 
GAAGCCATCT CTCACCAGAA ACTGGAGCCT GAGAGTGGCC TCTCTCCAAC TCCATCCCTG 
GACTGCAGCC CAAATCTTCG ATGCTTCACT TTTTCCAGAG GGGACGTCAG CCGCTTTCCT 
CCTGCCAGAC TTGGCAGCCT GGGTCTCATG GACTACTACC TGATGGATGC TGCCTCCTTG 
CTCCCTGTCC TGGCTCTTGG TCTGCAGCAT GGAGACACTG TGCTTGATCT GTGTGCTGCT 
CCTGGGGGCA AAACGCTGGC ATTACTTCAG ACGGGTTGTT GCCGTAATCT AGCTGCCAAC 
GACCTCTCCA CTTCCCGAAC AGGCAGACTG CAGAAGGTCC TTCACAGTTA TGTGCCTCAA 
GATATTAGGG AAGGGAACCA AGTCCGAGTT ACCTCATGGG ATGGCAGGAA GTGGGGAGAA 
CTGGAGGGGG ACACCTATGA TAGGGTGTTA GTAGATGTGC CATGTACCAC GGACCGCCAT 
TCCCTTCATG AGGAAGAGAA CAACATCTTT CAGCGGTCAA GGAAGAAGGA GCGACAGATG 
TTGCCTATGC TGCAGGTGCA GCTTCTTGCG GCTGGACTCC TTGCCACCAA ACCAGGAGGT 
CACGTTGTCT ATTCAACTTG CTCACTGTCT CACTTACAGA ATGAGTATGT GGTGCAAGGT 
GCCATTGAGC TTCTGGCCAA TCAATACAAC ATCAAGGTTC AAGTGGAAGA CCTGTCTCAC 
TTCCGAAAGC TTTTCATGGA CACATTCTGT TTCTTCCCAT CCTGCCAGGT TGGGGAGCTG 
GTAATACCAA ACCTCATGGT CAATTTTGGA CCTATGTACT TCTGCAAACT GCATAGGCTG 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082418.1
CDS	101..1246
Translation	MAAPVLRCVRKLLKLVDFTPVPRRYRYKKKWATTEPQFTASRLALQNFDMTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDSVSAKLEQLSAKDFVSEAISHQKLEPESGLSPTPSLDCSPNLRCFTFSRGDVSRFPPARLGSLGLMDYYLMDAASLLPVLALGLQHGDTVLDLCAAPGGKTLALLQTGCCRNLAANDLSTSRTGRLQKVLHSYVPQDIREGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFQRSRKKERQMLPMLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYNIKVQVEDLSHFRKLFMDTFCFFPSCQVGELVIPNLMVNFGPMYFCKLHRLP

Target ORF information:

RefSeq Version	NM_028142.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028142.4

ORF Insert Sequence:

ATGGCTGCGC CCGTCTTAAG ATGTGTTCGG AAGCTGCTGA AGCTCGTGGA CTTCACGCCG 
GTCCCGCGGA GATATCGATA TAAGAAGAAA TGGGCTACCA CAGAACCACA ATTCACGGCC 
AGTCGGCTTG CTCTGCAGAA TTTTGACATG ACCTACAGTG TACAGTTTGG GGATCTTTGG 
CCATCAATCC GTGTTAGTCT TCTCTCAGAG CAGAAGTATG GTGCATTGGT CAATAATTTT 
GCTGCTTGGG ATAGTGTGAG TGCTAAGCTG GAGCAGCTGA GTGCCAAGGA CTTCGTCAGT 
GAAGCCATCT CTCACCAGAA ACTGGAGCCT GAGAGTGGCC TCTCTCCAAC TCCATCCCTG 
GACTGCAGCC CAAATCTTCG ATGCTTCACT TTTTCCAGAG GGGACGTCAG CCGCTTTCCT 
CCTGCCAGAC TTGGCAGCCT GGGTCTCATG GACTACTACC TGATGGATGC TGCCTCCTTG 
CTCCCTGTCC TGGCTCTTGG TCTGCAGCAT GGAGACACTG TGCTTGATCT GTGTGCTGCT 
CCTGGGGGCA AAACGCTGGC ATTACTTCAG ACGGGTTGTT GCCGTAATCT AGCTGCCAAC 
GACCTCTCCA CTTCCCGAAC AGGCAGACTG CAGAAGGTCC TTCACAGTTA TGTGCCTCAA 
GATATTAGGG AAGGGAACCA AGTCCGAGTT ACCTCATGGG ATGGCAGGAA GTGGGGAGAA 
CTGGAGGGGG ACACCTATGA TAGGGTGTTA GTAGATGTGC CATGTACCAC GGACCGCCAT 
TCCCTTCATG AGGAAGAGAA CAACATCTTT CAGCGGTCAA GGAAGAAGGA GCGACAGATG 
TTGCCTATGC TGCAGGTGCA GCTTCTTGCG GCTGGACTCC TTGCCACCAA ACCAGGAGGT 
CACGTTGTCT ATTCAACTTG CTCACTGTCT CACTTACAGA ATGAGTATGT GGTGCAAGGT 
GCCATTGAGC TTCTGGCCAA TCAATACAAC ATCAAGGTTC AAGTGGAAGA CCTGTCTCAC 
TTCCGAAAGC TTTTCATGGA CACATTCTGT TTCTTCCCAT CCTGCCAGGT TGGGGAGCTG 
GTAATACCAA ACCTCATGGT CAATTTTGGA CCTATGTACT TCTGCAAACT GCATAGGCTG 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsun4 ( NM_028142.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082418.1 Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 1, mRNA.

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CloneID	OMu16191
Clone ID Related Accession (Same CDS sequence)	NM_028142.4 , NM_028142.5
Accession Version	NM_028142.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1552752000000
Organism	Mus musculus(house mouse)
Product	putative methyltransferase NSUN4 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627105.13. On Mar 18, 2019 this sequence version replaced NM_028142.4. Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.593927.1, SRR5189682.106883.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGCGC CCGTCTTAAG ATGTGTTCGG AAGCTGCTGA AGCTCGTGGA CTTCACGCCG 
GTCCCGCGGA GATATCGATA TAAGAAGAAA TGGGCTACCA CAGAACCACA ATTCACGGCC 
AGTCGGCTTG CTCTGCAGAA TTTTGACATG ACCTACAGTG TACAGTTTGG GGATCTTTGG 
CCATCAATCC GTGTTAGTCT TCTCTCAGAG CAGAAGTATG GTGCATTGGT CAATAATTTT 
GCTGCTTGGG ATAGTGTGAG TGCTAAGCTG GAGCAGCTGA GTGCCAAGGA CTTCGTCAGT 
GAAGCCATCT CTCACCAGAA ACTGGAGCCT GAGAGTGGCC TCTCTCCAAC TCCATCCCTG 
GACTGCAGCC CAAATCTTCG ATGCTTCACT TTTTCCAGAG GGGACGTCAG CCGCTTTCCT 
CCTGCCAGAC TTGGCAGCCT GGGTCTCATG GACTACTACC TGATGGATGC TGCCTCCTTG 
CTCCCTGTCC TGGCTCTTGG TCTGCAGCAT GGAGACACTG TGCTTGATCT GTGTGCTGCT 
CCTGGGGGCA AAACGCTGGC ATTACTTCAG ACGGGTTGTT GCCGTAATCT AGCTGCCAAC 
GACCTCTCCA CTTCCCGAAC AGGCAGACTG CAGAAGGTCC TTCACAGTTA TGTGCCTCAA 
GATATTAGGG AAGGGAACCA AGTCCGAGTT ACCTCATGGG ATGGCAGGAA GTGGGGAGAA 
CTGGAGGGGG ACACCTATGA TAGGGTGTTA GTAGATGTGC CATGTACCAC GGACCGCCAT 
TCCCTTCATG AGGAAGAGAA CAACATCTTT CAGCGGTCAA GGAAGAAGGA GCGACAGATG 
TTGCCTATGC TGCAGGTGCA GCTTCTTGCG GCTGGACTCC TTGCCACCAA ACCAGGAGGT 
CACGTTGTCT ATTCAACTTG CTCACTGTCT CACTTACAGA ATGAGTATGT GGTGCAAGGT 
GCCATTGAGC TTCTGGCCAA TCAATACAAC ATCAAGGTTC AAGTGGAAGA CCTGTCTCAC 
TTCCGAAAGC TTTTCATGGA CACATTCTGT TTCTTCCCAT CCTGCCAGGT TGGGGAGCTG 
GTAATACCAA ACCTCATGGT CAATTTTGGA CCTATGTACT TCTGCAAACT GCATAGGCTG 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082418.1
CDS	36..1181
Translation	MAAPVLRCVRKLLKLVDFTPVPRRYRYKKKWATTEPQFTASRLALQNFDMTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDSVSAKLEQLSAKDFVSEAISHQKLEPESGLSPTPSLDCSPNLRCFTFSRGDVSRFPPARLGSLGLMDYYLMDAASLLPVLALGLQHGDTVLDLCAAPGGKTLALLQTGCCRNLAANDLSTSRTGRLQKVLHSYVPQDIREGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFQRSRKKERQMLPMLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYNIKVQVEDLSHFRKLFMDTFCFFPSCQVGELVIPNLMVNFGPMYFCKLHRLP

Target ORF information:

RefSeq Version	NM_028142.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOL1/NOP2/Sun domain family, member 4 (Nsun4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028142.5

ORF Insert Sequence:

ATGGCTGCGC CCGTCTTAAG ATGTGTTCGG AAGCTGCTGA AGCTCGTGGA CTTCACGCCG 
GTCCCGCGGA GATATCGATA TAAGAAGAAA TGGGCTACCA CAGAACCACA ATTCACGGCC 
AGTCGGCTTG CTCTGCAGAA TTTTGACATG ACCTACAGTG TACAGTTTGG GGATCTTTGG 
CCATCAATCC GTGTTAGTCT TCTCTCAGAG CAGAAGTATG GTGCATTGGT CAATAATTTT 
GCTGCTTGGG ATAGTGTGAG TGCTAAGCTG GAGCAGCTGA GTGCCAAGGA CTTCGTCAGT 
GAAGCCATCT CTCACCAGAA ACTGGAGCCT GAGAGTGGCC TCTCTCCAAC TCCATCCCTG 
GACTGCAGCC CAAATCTTCG ATGCTTCACT TTTTCCAGAG GGGACGTCAG CCGCTTTCCT 
CCTGCCAGAC TTGGCAGCCT GGGTCTCATG GACTACTACC TGATGGATGC TGCCTCCTTG 
CTCCCTGTCC TGGCTCTTGG TCTGCAGCAT GGAGACACTG TGCTTGATCT GTGTGCTGCT 
CCTGGGGGCA AAACGCTGGC ATTACTTCAG ACGGGTTGTT GCCGTAATCT AGCTGCCAAC 
GACCTCTCCA CTTCCCGAAC AGGCAGACTG CAGAAGGTCC TTCACAGTTA TGTGCCTCAA 
GATATTAGGG AAGGGAACCA AGTCCGAGTT ACCTCATGGG ATGGCAGGAA GTGGGGAGAA 
CTGGAGGGGG ACACCTATGA TAGGGTGTTA GTAGATGTGC CATGTACCAC GGACCGCCAT 
TCCCTTCATG AGGAAGAGAA CAACATCTTT CAGCGGTCAA GGAAGAAGGA GCGACAGATG 
TTGCCTATGC TGCAGGTGCA GCTTCTTGCG GCTGGACTCC TTGCCACCAA ACCAGGAGGT 
CACGTTGTCT ATTCAACTTG CTCACTGTCT CACTTACAGA ATGAGTATGT GGTGCAAGGT 
GCCATTGAGC TTCTGGCCAA TCAATACAAC ATCAAGGTTC AAGTGGAAGA CCTGTCTCAC 
TTCCGAAAGC TTTTCATGGA CACATTCTGT TTCTTCCCAT CCTGCCAGGT TGGGGAGCTG 
GTAATACCAA ACCTCATGGT CAATTTTGGA CCTATGTACT TCTGCAAACT GCATAGGCTG 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NSUN4 is a dual function mitochondrial protein required for both methylation of 12S rRNA and coordination of mitoribosomal assembly.
PLoS genetics10(2)e1004110(2014 Feb)
Metodiev MD,Spåhr H,Loguercio Polosa P,Meharg C,Becker C,Altmueller J,Habermann B,Larsson NG,Ruzzenente B

Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase family genes in early mouse embryogenesis.
Gene expression patterns : GEP13(8)319-27(2013 Dec)
Chi L,Delgado-Olguín P

MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome.
Cell metabolism13(5)527-39(2011 May)
Cámara Y,Asin-Cayuela J,Park CB,Metodiev MD,Shi Y,Ruzzenente B,Kukat C,Habermann B,Wibom R,Hultenby K,Franz T,Erdjument-Bromage H,Tempst P,Hallberg BM,Gustafsson CM,Larsson NG

A novel protein, sperm head and tail associated protein (SHTAP), interacts with cysteine-rich secretory protein 2 (CRISP2) during spermatogenesis in the mouse.
Biology of the cell102(2)93-106(2009 Nov)
Jamsai D,Rijal S,Bianco DM,O'Connor AE,Merriner DJ,Smith SJ,Gibbs GM,O'Bryan MK

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Nsun4 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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